US20210299211A1

US20210299211A1 - Peptidomimetic macrocycles and formulations thereof

Info

Publication number: US20210299211A1
Application number: US17/091,267
Authority: US
Inventors: David Allen Annis; Krzysztof Darlak; Chris Rhodes; Sonoko Kanai; Joerg Hoernschemeyer; Michaela Grass
Original assignee: E Hoffman La Roche Inc; Aileron Therapeutics Inc
Current assignee: E Hoffman La Roche Inc; Aileron Therapeutics Inc
Priority date: 2014-09-24
Filing date: 2020-11-06
Publication date: 2021-09-30
Also published as: US10905739B2; WO2016049355A1; JP7219258B2; AU2015320545A1; AU2015320545B2; US20160101145A1; CN107106642A; KR102570210B1; IL251122A0; KR20170063798A; SG11201702175YA; CA2961029A1; JP2018503595A; BR112017005736A2; AU2015320545C1; US20230127248A1; SG10201902598VA; JP2022046771A; EP3197477A4; CN112972378A

Abstract

Aqueous pharmaceutical formulations, for parenteral administration, comprising peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof wherein the peptidomimetic macrocycle binds to MDM2 and/or MDMX proteins are disclosed. Also disclosed are methods of treating diseases and disorders using the aqueous pharmaceutical formulations disclosed herein.

Description

CROSS REFERENCE

This application is a continuation of U.S. application Ser. No. 14/864,687, filed Sep. 24, 2015, now patented as U.S. Pat. No. 10,905,739, issued Feb. 2, 2021, which claims priority to U.S. Provisional Application No. 62/054,842, filed Sep. 24, 2014, each of which is incorporated herein by reference in its entirety.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jun. 4, 2021, is named 35224-803.301_SL.txt and is 1,382,300 bytes in size.

BACKGROUND OF THE DISCLOSURE

The human transcription factor protein p53 induces cell cycle arrest and apoptosis in response to DNA damage and cellular stress, and thereby plays a critical role in protecting cells from malignant transformation. The E3 ubiquitin ligase MDM2 (also known as HDM2 or human double minute 2) negatively regulates p53 function through a direct binding interaction that neutralizes the p53 transactivation activity, leads to export from the nucleus of p53 protein, and targets p53 for degradation via the ubiquitylation-proteasomal pathway. Loss of p53 activity, either by deletion, mutation, or MDM2 overexpression, is the most common defect in human cancers. Tumors that express wild type p53 are vulnerable to pharmacologic agents that stabilize or increase the concentration of active p53. In this context, inhibition of the activities of MDM2 has emerged as a validated approach to restore p53 activity and resensitize cancer cells to apoptosis in vitro and in vivo. MDMX (also known as MDM4, HDM4 or human double minute 4) has more recently been identified as a similar negative regulator of p53, and studies have revealed significant structural homology between the p53 binding interfaces of MDM2 and MDMX. MDMX has also been observed to be overexpressed in human tumors. The p53-MDM2 and p53-MDMX protein-protein interactions are mediated by the same 15-residue alpha-helical transactivation domain of p53, which inserts into hydrophobic clefts on the surface of MDM2 and MDMX. Three residues within this domain of wild type p53 (F19, W23, and L26) are essential for binding to MDM2 and MDMX.
There remains a considerable need for compounds capable of binding to and modulating the activity of p53, MDM2 and/or MDMX. Provided herein are aqueous pharmaceutical formulations comprising p53-based peptidomimetic macrocycles that modulate an activity of p53. Also provided herein are aqueous pharmaceutical formulations comprising p53-based peptidomimetic macrocycles that inhibit the interactions between p53, MDM2 and/or MDMX proteins. Further, provided herein are aqueous pharmaceutical formulations comprising p53-based peptidomimetic macrocycles that can be used for treating diseases including but not limited to cancer and other hyperproliferative diseases.

SUMMARY OF THE DISCLOSURE

In one aspect, the disclosure provides an aqueous pharmaceutical formulation comprising a peptidomimetic macrocycle that binds to MDM2 and/or MDMX proteins or a pharmaceutically acceptable salt thereof, a buffering agent, a tonicity agent, and a stabilizing agent wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is equal to or greater than 15 mg/mL and wherein the aqueous pharmaceutical formulation comprises less than 2% w/v of any micelle forming agent. The micelle forming agent can be solutol-HS-15. In some examples, the peptidomimetic macrocycle forms a micelle in absence of a surfactant.
In another aspect, the disclosure provides an aqueous pharmaceutical formulation comprising (i) a peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof, wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is equal to or greater than 15 mg/mL; (ii) a buffering agent; (iii) a stabilizing agent; and (iv) a tonicity agent, wherein the molar ratio of the peptidomimetic macrocycle to the buffering agent is in the range of 0.01-2.5.
In another aspect, the disclosure provides an aqueous pharmaceutical formulation comprising a peptidomimetic macrocycle that binds to a target protein with a K_Dvalue of 1×10⁻⁷M or less, or a pharmaceutically acceptable salt thereof, a buffering agent, a tonicity agent, and a stabilizing agent wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is equal to or greater than 15 mg/mL and wherein the aqueous pharmaceutical formulation comprises less than 2% w/v of any micelle forming agent, wherein the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has: (a) a length value of from 10 to 24 amino acids, (b) a von Heijne value of from 2 to 10, (c) a net charge of from −4 to +2, (d) a percent alanine content of from 15% to 50%, (e) or any combination of (a)-(d).
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof is not precipitated in the formulation. In some embodiments, an aqueous solubility of the peptidomimetic macrocycle is determined by evaluating the turbidity of a solution comprising the peptidomimetic macrocycle. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an amphipathicity that falls in a range that is optimal for cell permeability.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a length value of from 14 to 20 amino acids. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a von Heijne value of from 2 to 9. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a von Heijne value of from 3 to 8. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a von Heijne value of from 4 to 7. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a net charge of from −2 to 0. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a percent alanine content of from 15% to 40%. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a percent alanine content of from 20% to 40%. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a percent alanine content of from 25% to 40%. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a length value of from 14 to 20 amino acids, a von Heijne value of from 4 to 7, a net charge of from −2 to 0, and a percent alanine content of from 25% to 40%. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof is soluble, does not have off-target effects, or a combination thereof.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises a first C-terminal amino acid that is hydrophobic. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises a second C-terminal amino acid that is hydrophobic. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises a third C-terminal amino acid that is hydrophobic. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises a fourth C-terminal amino acid that is hydrophobic. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises a fifth C-terminal amino acid that is hydrophobic. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises a sixth C-terminal amino acid that is hydrophobic.
In some embodiments, the first amino acid connected to the crosslinker is N-terminal to the second amino acid connected to the crosslinker, and wherein the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises 1, 2, 3, 4, 5, 6, 7, or 8 amino acids that are C-terminal to the second amino acid connected to the crosslinker.
In some embodiments, the first amino acid connected to the crosslinker is N-terminal to the second amino acid connected to the crosslinker, and wherein the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises 1, 2, 3, 4, 5, or 6 hydrophobic amino acids that are C-terminal to the second amino acid connected to the crosslinker.
In some embodiments, the first amino acid connected to the crosslinker is N-terminal to the second amino acid connected to the crosslinker, and wherein the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises 1, 2, or 3 glutamines that are C-terminal to the second amino acid connected to the crosslinker.
In some embodiments, the amino acid that is hydrophobic is a small hydrophobic amino acid. In some embodiments, the amino acid that is hydrophobic is an alanine, a D-alanine, or an Aib.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof is a helical polypeptide. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises an α-helix. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof comprises an amphipathic α-helix.
In some embodiments, the first amino acid connected to the crosslinker or the second amino acid connected to the crosslinker is an α,α-disubstituted amino acid. In some embodiments, the first amino acid connected to the crosslinker and the second amino acid connected to the crosslinker are α,α-disubstituted amino acids. In some embodiments, the first amino acid connected to the crosslinker and the second amino acid connected to the crosslinker are separated by two amino acids. In some embodiments, the first amino acid connected to the crosslinker and the second amino acid connected to the crosslinker are separated by three amino acids. In some embodiments, the first amino acid connected to the crosslinker and the second amino acid connected to the crosslinker are separated by six amino acids. In some embodiments, the crosslinker spans 1 turn of an α-helix of the peptidomimetic macrocycle. In some embodiments, the crosslinker spans 2 turns of an α-helix of the peptidomimetic macrocycle. In some embodiments, the length of the crosslinker is from about 5 Å to about 9 Å per turn of an α-helix of the peptidomimetic macrocycle. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof provides a therapeutic effect. In some embodiments, an ability of the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof to penetrate cell membranes by an energy-dependent process is improved relative to a corresponding uncrosslinked peptidomimetic macrocycle. In some embodiments, the ability of the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof to penetrate cell membranes by an energy-independent process is improved relative to a corresponding uncrosslinked peptidomimetic macrocycle. In some embodiments, the energy-dependent process is primary active transport, secondary transport, endocytosis, or a combination thereof. In some embodiments, the energy-dependent process is active transport. In some embodiments, the energy-independent process is passive diffusion, facilitated diffusion, filtration, or a combination thereof. In some embodiments, the energy-independent process is passive transport.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to HDM2 with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to HDM2 or HDM4 with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to HDM4 with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to a PB1 peptide binding site of a PA protein with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to a PB2 peptide binding site of a PB1 protein with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to viral polymerase, for example, a RNA-dependent RNA polymerase with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof inhibits an influenza RNA-dependent RNA polymerase. In some embodiments, the virus is influenza virus. In some embodiments, the peptidomimetic macrocycle is capable of competing with the binding of a peptide of the sequence MDVNPTLLFLKVPAQ (SEQ ID NO: 1) or MERIKELRNLM (SEQ ID NO: 2) to the viral RNA-dependent RNA polymerase. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to MCL-1, BCL-X_L, BCL-2, or a combination thereof with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to MCL-1 with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to BCL-X_Lwith a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof binds to BCL-2 with a K_Dvalue of 1×10⁻⁷M or less.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an IC₅₀value of 100 nM or less to a target protein. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an EC₅₀value of 100 μM or less.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an IC₅₀value of 10 nM or less to a target protein. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an EC₅₀value of 10 μM or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an IC₅₀value of 1 nM or less to a target protein. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has an EC₅₀value of 1 μM or less.
In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a penetration efficiency value of 100 or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a penetration efficiency value of 10 or less. In some embodiments, the peptidomimetic macrocycle or pharmaceutically acceptable salt thereof has a penetration efficiency value of 1 or less.
In some embodiments, the peptidomimetic macrocycle penetrates cell membranes by an energy-dependent process and binds to an intracellular target with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the energy-dependent process comprises primary active transport, secondary transport, or endocytosis. In some embodiments, the energy-dependent process comprises active transport. In some embodiments, the peptidomimetic macrocycle penetrates cell membranes by an energy-independent process and binds to an intracellular target with a K_Dvalue of 1×10⁻⁷M or less. In some embodiments, the energy-independent process comprises passive diffusion, facilitated diffusion, or filtration. In some embodiments, the energy-independent process comprises passive transport.
In some embodiments, the amount of the buffering agent in the aqueous pharmaceutical formulations of the disclosure is 0.001-10% w/v, the stabilizing agent in the aqueous pharmaceutical formulations of the disclosure is 0.001-10% w/v and, the amount of the tonicity agent in the aqueous pharmaceutical formulations of the disclosure 1.0-10% ow/v.
The pharmaceutically acceptable salt of the peptidomimetic macrocycle can be a sodium salt. In some examples, the pharmaceutically acceptable salt of the peptidomimetic macrocycle can be a potassium, lithium, calcium, zinc or magnesium salt.
Any suitable amount of the peptidomimetic macrocycle can be used in the aqueous pharmaceutical formulations of the disclosure. In some examples, the amount of the peptidomimetic macrocycle present in the aqueous pharmaceutical formulation can be from about 0.1-10% w/v. For example, the amount of the peptidomimetic macrocycle present in the aqueous pharmaceutical formulation can be about 1% w/v, 1.5% w/v, or 2% w/v. In some examples, the concentration of the peptidomimetic macrocycle present in the aqueous pharmaceutical formulation is about 15-100 mg/mL. In some examples, the concentration of the peptidomimetic macrocycle present in the aqueous pharmaceutical formulation is about 15-50 mg/mL. In some examples, the concentration of the peptidomimetic macrocycle present in the aqueous pharmaceutical formulation is about 15, 20, 25, or 50 mg/mL.
Any suitable buffering agent can be used in the aqueous pharmaceutical formulations described herein. In some examples, the buffering agent is selected from a group consisting of ammonia solution, calcium carbonate, tribasic calcium phosphate, citric acid dihydrate, citric acid monohydrate, dibasic sodium phosphate, diethanolamine, malic acid, monobasic sodium phosphate, monoethanolamine, monosodium glutamate, phosphoric acid, phosphate-citrate buffer (dibasic sodium phosphate and citric acid), potassium citrate, sodium acetate, sodium bicarbonate, sodium borate, sodium citrate dehydrate, sodium hydroxide, sodium lactate, sodium carbonate, and triethanolamine (tris(hydroxymethyl)aminomethane). In some examples, the buffering agent is a phosphate buffer. In some examples, the buffering agent is selected from a group consisting of phosphoric acid, dibasic sodium phosphate, monobasic sodium phosphate or a mixture thereof. In some examples, the buffering agent is 20 mM phosphate buffer
The amount of the buffering agent in the aqueous pharmaceutical formulations of the disclosure can be from about 0.001-10% w/v. In some examples, the amount of the buffering agent in the aqueous pharmaceutical formulations of the disclosure is from about 0.01-10% w/v. In some examples, the amount of the buffering agent in the aqueous pharmaceutical formulations of the disclosure is from about 0.01-5% w/v. In some examples, the amount of the buffering agent in the aqueous pharmaceutical formulations of the disclosure is from about 0.01-1% w/v. In some examples, the amount of the buffering agent present in the aqueous pharmaceutical formulations of the disclosure is about 0.2% w/v.
The stabilizing agent in the aqueous pharmaceutical formulations of the disclosure can be a non-ionic stabilizing agent. In some examples, the stabilizing agent is a fatty acid ester. In some examples, the stabilizing agent can be a surfactant. In some for examples, the stabilizing agent is a non-ionic surfactant. In some for examples, the stabilizing agent is an anti-oxidant. In some examples the stabilizing agent can be selected from a group consisting of polyoxyethylene glycol alkyl ethers, polyoxypropylene glycol alkyl ethers, glucoside alkyl ethers, polyoxyethylene glycol octylphenol ethers, polyoxyethylene glycol alkylphenol ethers, glycerol alkyl esters, polyoxyethylene glycol sorbitan alkyl esters, sorbitan alkyl esters, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide, block copolymers of polyethylene glycol and polypropylene glycol, and polyethoxylated tallow amine. In some examples, the stabilizing agent can be a polyoxyethylene sorbitan fatty acid ester. In some examples, stabilizing agent can be polysorbate 20, polysorbate 21, polysorbate 40, polysorbate 60, polysorbate 61, polysorbate 65, polysorbate 80, polysorbate 81, polysorbate 85 or polysorbate 120. In some examples, the stabilizing agent can be polysorbate 20.
The amount of the stabilizing agent present in the aqueous pharmaceutical formulation is from about 0.001-10% w/v, for example from about 0.01-0.05% w/v. In some examples, the amount of the stabilizing agent present in the aqueous pharmaceutical formulations is about 0.03% w/v. In some examples, the aqueous pharmaceutical formulations comprise 250-350 ppm polysorbate 20. The aqueous pharmaceutical formulation of the disclosure can be a solution. In some examples, the aqueous pharmaceutical formulations can be sterile. In some examples, the aqueous pharmaceutical formulations can be colorless. In some examples, the aqueous pharmaceutical formulations can be a frozen solution. In some examples, the aqueous pharmaceutical formulation can be refrigerated solution.
In some examples, the aqueous pharmaceutical formulations can be particulate-free. In some examples, the aqueous pharmaceutical formulations comprise less than about 6,000 particles of size ≥10 μm in about 5 mL of the aqueous formulation. In some examples, the aqueous pharmaceutical formulations comprise less than about 600 particles of size ≥25 μm in about 5 mL of the aqueous formulation.
In some examples, the aqueous pharmaceutical formulations are dissolved into a diluent prior to administration into a subject. The diluent can be water for injection. In some examples, thee diluent can be solution of dextrose in water. The amount of the diluent can be from about 50-99% w/v. In some examples, the amount of the diluent can be about 90% w/v.
In some examples, the tonicity agent in the aqueous pharmaceutical formulations of the disclosure can be a non-ionic tonicity agent. In some examples, the tonicity agent can be a sugar or a sugar alcohol. In some examples, the tonicity agent can be a mono- or a disaccharide. In some cases, the tonicity agent can be selected from a groups consisting of glucose, fructose, galactose, sucrose, lactose, maltose, trehalose, and mixtures thereof. In some examples, the tonicity agent can be mannitol, glycerin, or a combination thereof. In some examples the tonicity agent can be D-trehalose.
The amount of the tonicity agent present in the aqueous pharmaceutical formulations can be from about 1-15% w/v. In some examples, the amount of the tonicity agent present in the aqueous pharmaceutical formulations can be about 8% w/v. The concentration of the tonicity agent can be from about 200-300 mM. In some examples, the concentration of the tonicity agent is 240 mM.
The pH of the aqueous pharmaceutical formulations of the disclosure can be from about 4.0-9.0. In some examples the pH of the aqueous pharmaceutical formulations of the disclosure is from about 4.5-8.5. In some examples the pH of the aqueous pharmaceutical formulations of the disclosure is from about 5.0-8.0. In some examples the pH of the aqueous pharmaceutical formulations of the disclosure is from about 5.5-7.5. In some examples the pH of the aqueous pharmaceutical formulations of the disclosure is from about 7.0-7.5.
The aqueous pharmaceutical formulations of the disclosure can be stable for at least two years at a temperature of about −20° C.-25° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least one year at a temperature of about −20° C.-25° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least 6 months at a temperature of about −20° C.-25° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least 3 months at a temperature of about −20° C.-25° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least 3 months at a temperature of about 45° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least 6 months at a temperature of about 45° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least 3 weeks at a temperature of about 75° C. In some examples, the aqueous pharmaceutical formulations can be stable for at least 1.5 weeks at a temperature of about 75° C.
In some examples, the aqueous pharmaceutical formulations upon storage for 24 months at from about 2° C.-8° C. can comprise at least 95% of the initial amount of the peptidomimetic macrocycle. In some examples, the aqueous pharmaceutical formulations upon storage for 12 months at from about 2° C.-8° C. can comprise at least 95% of the initial amount of the peptidomimetic macrocycle. In some examples, the aqueous pharmaceutical formulations upon storage for 6 months at from about 2° C.-8° C. can comprise at least 95% of the initial amount of the peptidomimetic macrocycle. In some cases, the aqueous pharmaceutical formulations upon storage for 3 months at from about 2° C.-8° C. can comprise at least 95% of the initial amount of the peptidomimetic macrocycle.
The osmolality of the aqueous pharmaceutical formulations of the disclosure can be from about 100-600 milliosmoles per kilogram, for example from about 220-400 milliosmoles per kilogram.
The endotoxin level of the aqueous pharmaceutical formulations of the disclosure can be at most 2.0, 4.0, 6.0, 8.0 or 10 EU/mL². In some examples, the endotoxin level of the aqueous pharmaceutical formulations can be at most 4.5 EU/mL².
The aqueous pharmaceutical formulations of the disclosure can be contained in a container. The container can be a single use container or a multi-use container. In some examples, the container can be a glass vial. In some examples, the container is a pre-filled syringe to be used alone or in an injection device. In some examples, the container is a cartridge for a pen injection system, or a glass ampoule. In some examples, the container is a 20 mL, 10 mL, or a 5 mL glass serum vial. The glass vial can comprise borosilicate glass or polycarbonate. The container can comprise stopper and/or cap. The stopper can be a rubber stopper. The container can comprise a seal for example an aluminum seal.
The aqueous pharmaceutical formulations of the disclosure can be prepared by adding the peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof to water or an aqueous solution, wherein the peptidomimetic macrocycle is capable of binding to the MDM2 and/or MDMX proteins. The pharmaceutically acceptable salt can be a sodium salt, potassium salt or calcium salt. In some examples, the aqueous pharmaceutical formulations can be prepared by dissolving a sodium salt of the peptidomimetic macrocycle in water. The method can further comprise adding a buffering agent and a stabilizing agent.
The aqueous pharmaceutical formulations of the disclosure can be suitable for administration to a subject without reconstitution or dilution. In some examples, the aqueous pharmaceutical formulations can require reconstitution prior to administration to a subject. Reconstitution can involve dilution with an aqueous solution, for example with a solution of dextrose in water.
In some embodiments, the micelle forming agent in solutol-HS-15. In some embodiments, the peptidomimetic macrocycle forms a micelle in absence of a surfactant. In some embodiments, the aqueous pharmaceutical formulation does not form micelle.
The aqueous pharmaceutical formulations of the disclosure can further comprise a preservative. The preservative can be selected from a group consisting of benzalkonium chloride, EDTA and combination thereof.
In some examples, the preservative can be selected from a group consisting of phenol, meta-cresol and combination thereof.
The aqueous pharmaceutical formulations of the disclosure can further comprise a co-solvent. The co-solvent can be selected from a group consisting of dimethyl sulfoxide (DMSO), N-methylpyrrolidone (NMP), dimethylacetamide (DMA) and combinations thereof.
The molecular weight of the peptidomimetic macrocycle can be in the range of 1800-2000 D. In some examples, the peptidomimetic macrocycle has an observed mass (m/e) in the range of 900-1000 D.
In another aspect the disclosure provides an aqueous pharmaceutical formulation comprising a peptidomimetic macrocycle that binds to MDM2 and/or MDMX proteins or a pharmaceutically acceptable salt thereof, phosphate buffering agent, D-trehalose, and polysorbate 20, wherein the peptidomimetic macrocycle comprises an amino acid sequence which is at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% identical to an amino acid sequence in any of Table 1, Table 1a, Table 1b, and Table 1c, In some examples, the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation can be equal to or greater than 15 mg/mL. In some examples, the amount of D-trehalose in the aqueous pharmaceutical formulations can be about 8% w/v. The amount of polysorbate 20 in the aqueous pharmaceutical formulations can be about 0.03% w/v. In some examples, the aqueous pharmaceutical formulations comprise less than 2% w/v of any micelle forming agent.
The peptidomimetic macrocycle in the aqueous pharmaceutical formulations can comprise an amino acid sequence which is at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% identical to an amino acid sequence in any of Table 1, Table 1a, Table 1b, and Table 1c, and wherein the peptidomimetic macrocycle has the formula:

- wherein:
- each A, C, and D is independently an amino acid;
- each B is independently an amino acid,

[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];

- each E is independently an amino acid selected from the group consisting of Ala (alanine), D-Ala (D-alanine), Aib (α-aminoisobutyric acid), Sar (N-methyl glycine), and Ser (serine);
- each R₁and R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo; or forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids;
- each R₃independently is hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
- each L and L′ is independently a macrocycle-forming linker;

each L₃is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
each v is independently an integer from 1-1000;
each w is independently an integer from 3-1000;
u is an integer from 1-10;
each x, y and z is independently an integer from 0-10; and
each n is independently an integer from 1-5.
In some embodiments, the peptidomimetic macrocycle has formula:
wherein:
each of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀is individually an amino acid, wherein at least three of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3) or Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4), where each X is an amino acid; each D and E is independently an amino acid;
each R₁and R₂are independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-; or at least one of R₁and R₂forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids; each L or L′ is independently a macrocycle-forming linker;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
R₇is —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
R₈is —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
v is an integer from 1-1000; and
w is an integer from 0-1000.
In some examples, at least one of the macrocycle-forming linker in the Formulas provided herein has a formula -L₁-L₂-, wherein
L₁and L₂are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅; each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃; and
each R₃independently is hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
each n is independently an integer from 1-5.
In some embodiments w is an integer from 3-1000, for example 3-500, 3-200, 3-100, 3-50, 3-30, 3-20, or 3-10. In some examples, Xaa₅is Glu or an amino acid analog thereof. In some examples each E is independently an amino acid selected from Ala (alanine), D-Ala (D-alanine), Aib (α-aminoisobutyric acid), Sar (N-methyl glycine), and Ser (serine). In some examples, [D]_vis -Leu₁-Thr₂. In some examples, w is 3-6. In some examples, w is 6-10. In some examples, w is 6. In some examples, v is 1-10. In some examples, v is 2-10. In some examples, v is 2-5. In some examples, v is 2.
In some examples, L₁and L₂in the Formulas above are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, or heterocycloarylene, each being optionally substituted with R₅. In some examples, L₁and L₂are independently alkylene or alkenylene. In some examples, L is alkylene, alkenylene, or alkynylene. In some examples, L is alkylene. In some examples, L is C₃-C₁₆alkylene. In some examples, L is C₁₀-C₁₄alkylene.
In some examples, R₁and R₂in the Formulas above are independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-. In some examples, R₁and R₂are H. In some examples, R₁and R₂are independently alkyl. In some examples, R₁and R₂are methyl.
In some examples, x+y+z in the Formulas here is 6.
In some examples, in the Formulas here, u is 1.
In some examples, each E is Ser or Ala or an analog thereof.
In some examples, the aqueous pharmaceutical formulations comprise at least one amino acid which is an amino acid analog.
In some examples, the peptidomimetic macrocycle in the aqueous pharmaceutical formulations is a peptidomimetic macrocycle shown in Table 1c.
In another aspect, the disclosure provides a method of treating cancer in a subject, the method comprising administering to the subject a therapeutically effective amount of an aqueous pharmaceutical formulation of the disclosure.
In another aspect, the disclosure provides a method of treating cancer in a subject comprising administering to the subject an aqueous pharmaceutical formulation a peptidomimetic macrocycle peptidomimetic macrocycle that is capable of binding to the MDM2 and/or MDMX proteins and wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is greater than 15 mg/mL and wherein the aqueous pharmaceutical formulation contains less than 2% w/v of any micelle forming agent. The aqueous pharmaceutical formulation can further comprise a buffering agent, a stabilizing agent, and/or tonicity agent.
The cancer can be selected from the group consisting of head and neck cancer, melanoma, lung cancer, breast cancer, and glioma. In some examples, the cancer is selected from a group consisting of bladder cancer, bone cancer, breast cancer, cervical cancer, CNS cancer, colon cancer, ocular tumor, renal cancer, liver cancer, lung cancer, pancreatic cancer, choriocarcinoma (tumor of the placenta), prostate cancer, sarcoma, skin cancer, soft tissue cancer, gastric cancer, gall bladder cancer, biliary cancer, renal cancer, neoblastoma, or neuroendocrine cancer.
In another aspect, the disclosure provides a method of modulating the activity of p53 and/or MDM2 and/or MDMX in a subject comprising administering to the subject an aqueous pharmaceutical formulation comprising a peptidomimetic macrocycle capable of binding to the MDM2 and/or MDMX proteins, wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is greater than 15 mg/mL and wherein the aqueous pharmaceutical formulation contains less than 2% w/v of any micelle forming agent. The aqueous pharmaceutical formulation can further comprise a buffering agent, a tonicity agent, and/or a stabilizing agent.
In another aspect, the disclosure provides a method of antagonizing the interaction between p53 and MDM2 and/or between p53 and MDMX proteins in a subject, the method comprising administering to the subject a aqueous pharmaceutical formulation comprising a peptidomimetic macrocycle capable of binding to the MDM2 and/or MDMX proteins, wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is greater than 15 mg/mL and wherein the aqueous pharmaceutical formulation contains less than 2% w/v of any micelle forming agent. The aqueous pharmaceutical formulation can further comprise a buffering agent, a stabilizing agent and/or a tonicity agent.
In another aspect, the disclosure provides a method of making an aqueous pharmaceutical formulation comprising adding greater than 15 mg/mL of a peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof to water or an aqueous solution, wherein the peptidomimetic macrocycle is capable of binding to the MDM2 and/or MDMX proteins and wherein the aqueous pharmaceutical formulation comprises less than 2% w/v of any micelle forming agent. In some examples, the method comprises adding a sodium salt of the peptidomimetic macrocycle to water or an aqueous solution. The aqueous solution can comprise a buffering agent. The aqueous solution can also comprise a tonicity agent. The aqueous solution can further comprise a stabilizing agent.
The method can further comprise adjusting the pH of the solution comprising the buffering agent and the stabilizing agent during the addition of the peptidomimetic macrocycle. The pH can be adjusted by addition of a pH adjusting agent. In some examples, the pH is adjusted to be in the range of from about 6.0-8.0.
The amount of the pH adjusting agent added can be from about 0.01-10% w/v, for example about 0.09% w/v. The pH adjusting agent can comprise an acid or a base. In some examples, the pH adjusting agent comprises phosphoric acid. In some examples, the pH adjusting agent comprises sodium hydroxide, for example 0.1 N NaOH.
The method can further comprise filtration of the aqueous pharmaceutical formulation obtained after the addition of the peptidomimetic macrocycle to the aqueous solution. The filtration is performed under vacuum or under pressure. The filtration can comprise sterilizing filtration. In some examples, the filtration comprises use of membrane filter. In some examples, the membrane filter comprises cellulose or cellulose derivative, cellulosic ester (MCE), comprise polytetrafluoroethylene (PTFE), polyvinylidene, polyvinylidene chloride, or polyvinylidene fluoride. The membrane filter can have a pore size in the range from about 10 nm-10 μm, for example 0.2 μm. The filtration can result in clarification of the aqueous formulation. The filtering can involve passing the aqueous pharmaceutical formulation through one or more membrane filters.
In another aspect, the disclosure provides a kit comprising, in suitable container means, an aqueous pharmaceutical formulation comprising a peptidomimetic macrocycle and instructions for administration of the aqueous pharmaceutical formulation to a human subject, wherein the peptidomimetic macrocycle is capable of binding to MDM2 and/or MDMX proteins and wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is greater than 15 mg/mL and the aqueous pharmaceutical formulation comprises less than 2% w/v of any micelle forming agent. The instructions can be for intravenous administration of the aqueous formulation.
In some embodiments, the amount of aqueous pharmaceutical formulation made is about 1 liter to about 100 liters. In some embodiments, the amount of aqueous pharmaceutical formulation made is about 10 litres to about 100 litres. In some embodiments the amount of aqueous pharmaceutical formulation made is about 10 liters to about 50 liters.
A kit for formulating an aqueous pharmaceutical formulation comprising, in suitable container means, a peptidomimetic macrocycle capable of binding to the MDM2 and/or MDMX proteins or a pharmaceutically acceptable salt thereof, wherein the amount of the peptidomimetic macrocycle in the aqueous pharmaceutical formulation is greater than 15 mg/mL and the aqueous pharmaceutical formulation comprises less than 2% w/v of any micelle forming agent.

INCORPORATION BY REFERENCE

All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.

BRIEF DESCRIPTION OF THE DRAWINGS

The novel features of the disclosure are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present disclosure will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the disclosure are utilized, and the accompanying drawings of which:

FIG. 1. Shows a flow diagram of the manufacturing process of an exemplary aqueous pharmaceutical formulation of the disclosure.

FIG. 2. Shows the observation pictures for Example 5.

FIG. 3 Shows a plot of viable SJSA-1 cells (%) vs. log concentration (μM) of indicated peptide after incubation of the cells with the peptide for 72 hr in 10% serum.

FIG. 4 Shows the 12-month stability results for Aileron peptide-1. The data support greater than 2 year shelf life at −20-5° C.

DETAILED DESCRIPTION OF THE DISCLOSURE

While preferred embodiments of the present disclosure have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the disclosure. It should be understood that various alternatives to the embodiments of the disclosure described herein can be employed in practicing the disclosure. It is intended that the following claims define the scope of the disclosure and that methods and structures within the scope of these claims and their equivalents be covered thereby.

Definitions

As used herein, the term “macrocycle” refers to a molecule having a chemical structure including a ring or cycle formed by at least 9 covalently bonded atoms.
As used herein, the term “peptidomimetic macrocycle” or “crosslinked polypeptide” refers to a compound comprising a plurality of amino acid residues joined by a plurality of peptide bonds and at least one macrocycle-forming linker which forms a macrocycle between a first naturally-occurring or non-naturally-occurring amino acid residue (or analog) and a second naturally-occurring or non-naturally-occurring amino acid residue (or analog) within the same molecule. Peptidomimetic macrocycle include embodiments where the macrocycle-forming linker connects the α carbon of the first amino acid residue (or analog) to the α carbon of the second amino acid residue (or analog). The peptidomimetic macrocycles optionally include one or more non-peptide bonds between one or more amino acid residues and/or amino acid analog residues, and optionally include one or more non-naturally-occurring amino acid residues or amino acid analog residues in addition to any which form the macrocycle. A “corresponding uncrosslinked polypeptide” when referred to in the context of a peptidomimetic macrocycle is understood to relate to a polypeptide of the same length as the macrocycle and comprising the equivalent natural amino acids of the wild-type sequence corresponding to the macrocycle.
As used herein, the term “pharmaceutically acceptable salt” refers to those salts which are suitable for pharmaceutical use, preferably for use in humans and lower animals without undue irritation, allergic response and the like. Pharmaceutically acceptable salts of amines, carboxylic acids, and other types of compounds, are well known in the art. For example, S. M. Berge, et al., describe pharmaceutically acceptable salts in detail in J Pharmaceutical Sciences, 66: 1-19 (1977), incorporated herein by reference. The salts can be prepared in situ during the final isolation and purification of the peptidomimetic macrocycles of the invention, or separately by reacting a free base or free acid function with a suitable reagent, as described generally below. For example, a free base function can be reacted with a suitable acid. Suitable pharmaceutically acceptable salts can, include metal salts such as alkali metal salts, e. g. sodium, potassium, and lithium salts; and alkaline earth metal salts, e. g. calcium or magnesium salts. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like.
As used herein, the term “stability” can refer to chemical stability and/or physical stability. As used herein, the phrase chemical stability means the ability of a compound to maintain its chemical identity over time.
Accordingly, stability implies the ability of a chemical species to resist oxidation or other degradation, for example. As used herein, the phrase physical stability means the ability of a composition to maintain consistent physical properties over time. The ability of a composition to maintain a consistent disintegration time over time is exemplary of physical stability. In some embodiments, stability can also refer to the maintenance of a defined secondary structure in solution by a peptidomimetic macrocycle as measured by circular dichroism, NMR or another biophysical measure, or resistance to proteolytic degradation in vitro or in vivo. Non-limiting examples of secondary structures contemplated herein are α-helices, 3₁₀helices, β-turns, and β-pleated sheets.
The term “amino acid” refers to a molecule containing both an amino group and a carboxyl group. Suitable amino acids include, without limitation, both the D- and L-isomers of the naturally-occurring amino acids, as well as non-naturally occurring amino acids prepared by organic synthesis or other metabolic routes. The term amino acid, as used herein, includes, without limitation, α-amino acids, natural amino acids, non-natural amino acids, and amino acid analogs.
The term “α-amino acid” refers to a molecule containing both an amino group and a carboxyl group bound to a carbon which is designated the α-carbon.
The term “β-amino acid” refers to a molecule containing both an amino group and a carboxyl group in a β configuration.
The term “naturally occurring amino acid” refers to any one of the twenty amino acids commonly found in peptides synthesized in nature, and known by the one letter abbreviations A, R, N, C, D, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y and V.
The following table shows a summary of the properties of natural amino acids:


				Side-
			Side-	chain	Hydro-
	3-Letter	1-Letter	chain	charge	pathy
Amino Acid	Code	Code	Polarity	(pH 7.4)	Index

Alanine	Ala	A	nonpolar	neutral	1.8
Arginine	Arg	R	polar	positive	−4.5
Asparagine	Asn	N	polar	neutral	−3.5
Aspartic acid	Asp	D	polar	negative	−3.5
Cysteine	Cys	C	polar	neutral	2.5
Glutamic acid	Glu	E	polar	negative	−3.5
Glutamine	Gln	Q	polar	neutral	−3.5
Glycine	Gly	G	nonpolar	neutral	−0.4
Histidine	His	H	polar	positive (10%)	−3.2
				neutral (90%)
Isoleucine	Ile	I	nonpolar	neutral	4.5
Leucine	Leu	L	nonpolar	neutral	3.8
Lysine	Lys	K	polar	positive	−3.9
Methionine	Met	M	nonpolar	neutral	1.9
Phenylalanine	Phe	F	nonpolar	neutral	2.8
Proline	Pro	P	nonpolar	neutral	−1.6
Serine	Ser	S	polar	neutral	−0.8
Threonine	Thr	T	polar	neutral	−0.7
Tryptophan	Trp	W	nonpolar	neutral	−0.9
Tyrosine	Tyr	Y	polar	neutral	−1.3
Valine	Val	V	nonpolar	neutral	4.2

“Hydrophobic amino acids” include small hydrophobic amino acids and large hydrophobic amino acids. “Small hydrophobic amino acid” are glycine, alanine, proline, and analogs thereof. “Large hydrophobic amino acids” are valine, leucine, isoleucine, phenylalanine, methionine, tryptophan, and analogs thereof “Polar amino acids” are serine, threonine, asparagine, glutamine, cysteine, tyrosine, and analogs thereof. “Charged amino acids” are lysine, arginine, histidine, aspartate, glutamate, and analogs thereof.
The term “amino acid analog” refers to a molecule which is structurally similar to an amino acid and which can be substituted for an amino acid in the formation of a peptidomimetic macrocycle. Amino acid analogs include, without limitation, R-amino acids and amino acids where the amino or carboxy group is substituted by a similarly reactive group (e.g., substitution of the primary amine with a secondary or tertiary amine, or substitution of the carboxy group with an ester).
The term “non-natural amino acid” refers to an amino acid which is not one of the twenty amino acids commonly found in peptides synthesized in nature, and known by the one letter abbreviations A, R, N, C, D, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y and V. Non-natural amino acids or amino acid analogs include, without limitation, structures according to the following:
Amino acid analogs include β-amino acid analogs. Examples of β-amino acid analogs include, but are not limited to, the following: cyclic β-amino acid analogs; β-alanine; (R)-β-phenylalanine; (R)-1,2,3,4-tetrahydro-isoquinoline-3-acetic acid; (R)-3-amino-4-(1-naphthyl)-butyric acid; (R)-3-amino-4-(2,4-dichlorophenyl)butyric acid; (R)-3-amino-4-(2-chlorophenyl)-butyric acid; (R)-3-amino-4-(2-cyanophenyl)-butyric acid; (R)-3-amino-4-(2-fluorophenyl)-butyric acid; (R)-3-amino-4-(2-furyl)-butyric acid; (R)-3-amino-4-(2-methylphenyl)-butyric acid; (R)-3-amino-4-(2-naphthyl)-butyric acid; (R)-3-amino-4-(2-thienyl)-butyric acid; (R)-3-amino-4-(2-trifluoromethylphenyl)-butyric acid; (R)-3-amino-4-(3,4-dichlorophenyl)butyric acid; (R)-3-amino-4-(3,4-difluorophenyl)butyric acid; (R)-3-amino-4-(3-benzothienyl)-butyric acid; (R)-3-amino-4-(3-chlorophenyl)-butyric acid; (R)-3-amino-4-(3-cyanophenyl)-butyric acid; (R)-3-amino-4-(3-fluorophenyl)-butyric acid; (R)-3-amino-4-(3-methylphenyl)-butyric acid; (R)-3-amino-4-(3-pyridyl)-butyric acid; (R)-3-amino-4-(3-thienyl)-butyric acid; (R)-3-amino-4-(3-trifluoromethylphenyl)-butyric acid; (R)-3-amino-4-(4-bromophenyl)-butyric acid; (R)-3-amino-4-(4-chlorophenyl)-butyric acid; (R)-3-amino-4-(4-cyanophenyl)-butyric acid; (R)-3-amino-4-(4-fluorophenyl)-butyric acid; (R)-3-amino-4-(4-iodophenyl)-butyric acid; (R)-3-amino-4-(4-methylphenyl)-butyric acid; (R)-3-amino-4-(4-nitrophenyl)-butyric acid; (R)-3-amino-4-(4-pyridyl)-butyric acid; (R)-3-amino-4-(4-trifluoromethylphenyl)-butyric acid; (R)-3-amino-4-pentafluoro-phenylbutyric acid; (R)-3-amino-5-hexenoic acid; (R)-3-amino-5-hexynoic acid; (R)-3-amino-5-phenylpentanoic acid; (R)-3-amino-6-phenyl-5-hexenoic acid; (S)-1,2,3,4-tetrahydro-isoquinoline-3-acetic acid; (S)-3-amino-4-(1-naphthyl)-butyric acid; (S)-3-amino-4-(2,4-dichlorophenyl)butyric acid; (S)-3-amino-4-(2-chlorophenyl)-butyric acid; (S)-3-amino-4-(2-cyanophenyl)-butyric acid; (S)-3-amino-4-(2-fluorophenyl)-butyric acid; (S)-3-amino-4-(2-furyl)-butyric acid; (S)-3-amino-4-(2-methylphenyl)-butyric acid; (S)-3-amino-4-(2-naphthyl)-butyric acid; (S)-3-amino-4-(2-thienyl)-butyric acid; (S)-3-amino-4-(2-trifluoromethylphenyl)-butyric acid;
(S)-3-amino-4-(3,4-dichlorophenyl)butyric acid; (S)-3-amino-4-(3,4-difluorophenyl)butyric acid; (S)-3-amino-4-(3-benzothienyl)-butyric acid; (S)-3-amino-4-(3-chlorophenyl)-butyric acid; (S)-3-amino-4-(3-cyanophenyl)-butyric acid; (S)-3-amino-4-(3-fluorophenyl)-butyric acid; (S)-3-amino-4-(3-methylphenyl)-butyric acid; (S)-3-amino-4-(3-pyridyl)-butyric acid; (S)-3-amino-4-(3-thienyl)-butyric acid; (S)-3-amino-4-(3-trifluoromethylphenyl)-butyric acid; (S)-3-amino-4-(4-bromophenyl)-butyric acid; (S)-3-amino-4-(4-chlorophenyl)-butyric acid; (S)-3-amino-4-(4-cyanophenyl)-butyric acid; (S)-3-amino-4-(4-fluorophenyl)-butyric acid; (S)-3-amino-4-(4-iodophenyl)-butyric acid; (S)-3-amino-4-(4-methylphenyl)-butyric acid; (S)-3-amino-4-(4-nitrophenyl)-butyric acid; (S)-3-amino-4-(4-pyridyl)-butyric acid; (S)-3-amino-4-(4-trifluoromethylphenyl)-butyric acid; (S)-3-amino-4-pentafluoro-phenylbutyric acid; (S)-3-amino-5-hexenoic acid; (S)-3-amino-5-hexynoic acid; (S)-3-amino-5-phenylpentanoic acid; (S)-3-amino-6-phenyl-5-hexenoic acid; 1,2,5,6-tetrahydropyridine-3-carboxylic acid; 1,2,5,6-tetrahydropyridine-4-carboxylic acid; 3-amino-3-(2-chlorophenyl)-propionic acid; 3-amino-3-(2-thienyl)-propionic acid; 3-amino-3-(3-bromophenyl)-propionic acid; 3-amino-3-(4-chlorophenyl)-propionic acid; 3-amino-3-(4-methoxyphenyl)-propionic acid; 3-amino-4,4,4-trifluoro-butyric acid; 3-aminoadipic acid; D-β-phenylalanine; β-leucine; L-β-homoalanine; L-β-homoaspartic acid γ-benzyl ester; L-β-homoglutamic acid δ-benzyl ester; L-β-homoisoleucine; L-β-homoleucine; L-β-homomethionine; L-β-homophenylalanine; L-β-homoproline; L-β-homotryptophan; L-β-homovaline; L-Nω-benzyloxycarbonyl-β-homolysine; Nω-L-β-homoarginine; O-benzyl-L-β-homohydroxyproline; O-benzyl-L-β-homoserine; O-benzyl-L-β-homothreonine; O-benzyl-L-β-homotyrosine; γ-trityl-L-β-homoasparagine; (R)-β-phenylalanine; L-β-homoaspartic acid γ-t-butyl ester; L-β-homoglutamic acid δ-t-butyl ester; L-Nω-β-homolysine; Nδ-trityl-L-β-homoglutamine; Nω-2,2,4,6,7-pentamethyl-dihydrobenzofuran-5-sulfonyl-L-β-homoarginine; O-t-butyl-L-β-homohydroxy-proline; O-t-butyl-L-β-homoserine; O-t-butyl-L-β-homothreonine; O-t-butyl-L-β-homotyrosine; 2-aminocyclopentane carboxylic acid; and 2-aminocyclohexane carboxylic acid.
Amino acid analogs include analogs of alanine, valine, glycine or leucine. Examples of amino acid analogs of alanine, valine, glycine, and leucine include, but are not limited to, the following: α-methoxyglycine; α-allyl-L-alanine; α-aminoisobutyric acid; α-methyl-leucine; β-(1-naphthyl)-D-alanine; β-(1-naphthyl)-L-alanine; β-(2-naphthyl)-D-alanine; β-(2-naphthyl)-L-alanine; β-(2-pyridyl)-D-alanine; β-(2-pyridyl)-L-alanine; β-(2-thienyl)-D-alanine; β-(2-thienyl)-L-alanine; β-(3-benzothienyl)-D-alanine; β-(3-benzothienyl)-L-alanine; β-(3-pyridyl)-D-alanine; β-(3-pyridyl)-L-alanine; β-(4-pyridyl)-D-alanine; β-(4-pyridyl)-L-alanine; β-chloro-L-alanine; β-cyano-L-alanin; β-cyclohexyl-D-alanine; β-cyclohexyl-L-alanine; β-cyclopenten-1-yl-alanine; β-cyclopentyl-alanine; β-cyclopropyl-L-Ala-OH.dicyclohexylammonium salt; β-t-butyl-D-alanine; β-t-butyl-L-alanine; γ-aminobutyric acid; L-α,β-diaminopropionic acid; 2,4-dinitro-phenylglycine; 2,5-dihydro-D-phenylglycine; 2-amino-4,4,4-trifluorobutyric acid; 2-fluoro-phenylglycine; 3-amino-4,4,4-trifluoro-butyric acid; 3-fluoro-valine; 4,4,4-trifluoro-valine; 4,5-dehydro-L-leu-OH.dicyclohexylammonium salt; 4-fluoro-D-phenylglycine; 4-fluoro-L-phenylglycine; 4-hydroxy-D-phenylglycine; 5,5,5-trifluoro-leucine; 6-aminohexanoic acid; cyclopentyl-D-Gly-OH.dicyclohexylammonium salt; cyclopentyl-Gly-OH.dicyclohexylammonium salt; D-α,β-diaminopropionic acid; D-α-aminobutyric acid; D-α-t-butylglycine; D-(2-thienyl)glycine; D-(3-thienyl)glycine; D-2-aminocaproic acid; D-2-indanylglycine; D-allylglycine.dicyclohexylammonium salt; D-cyclohexylglycine; D-norvaline; D-phenylglycine; β-aminobutyric acid; β-aminoisobutyric acid; (2-bromophenyl)glycine; (2-methoxyphenyl)glycine; (2-methylphenyl)glycine; (2-thiazoyl)glycine; (2-thienyl)glycine; 2-amino-3-(dimethylamino)-propionic acid; L-α,β-diaminopropionic acid; L-α-aminobutyric acid; L-α-t-butylglycine; L-(3-thienyl)glycine; L-2-amino-3-(dimethylamino)-propionic acid; L-2-aminocaproic acid dicyclohexyl-ammonium salt; L-2-indanylglycine; L-allylglycine.dicyclohexyl ammonium salt; L-cyclohexylglycine; L-phenylglycine; L-propargylglycine; L-norvaline; N-α-aminomethyl-L-alanine; D-α,γ-diaminobutyric acid; L-α,γ-diaminobutyric acid; β-cyclopropyl-L-alanine; (N-β-(2,4-dinitrophenyl))-L-α,β-diaminopropionic acid; (N-β-1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl)-D-α,β-diaminopropionic acid; (N-β-1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl)-L-α,β-diaminopropionic acid; (N-β-4-methyltrityl)-L-α,β-diaminopropionic acid; (N-β-allyloxycarbonyl)-L-α,β-diaminopropionic acid; (N-γ-1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl)-D-α,γ-diaminobutyric acid; (N-γ-1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl)-L-α,γ-diaminobutyric acid; (N-γ-4-methyltrityl)-D-α,γ-diaminobutyric acid; (N-γ-4-methyltrityl)-L-α,γ-diaminobutyric acid; (N-γ-allyloxycarbonyl)-L-α,γ-diaminobutyric acid; D-α,γ-diaminobutyric acid; 4,5-dehydro-L-leucine; cyclopentyl-D-Gly-OH; cyclopentyl-Gly-OH; D-allylglycine; D-homocyclohexylalanine; L-1-pyrenylalanine; L-2-aminocaproic acid; L-allylglycine; L-homocyclohexylalanine; and N-(2-hydroxy-4-methoxy-Bzl)-Gly-OH.
Amino acid analogs include analogs of arginine or lysine. Examples of amino acid analogs of arginine and lysine include, but are not limited to, the following: citrulline; L-2-amino-3-guanidinopropionic acid; L-2-amino-3-ureidopropionic acid; L-citrulline; Lys(Me)₂-OH; Lys(N₃)—OH; Nδ-benzyloxycarbonyl-L-ornithine; Nω-nitro-D-arginine; Nω-nitro-L-arginine; α-methyl-omithine; 2,6-diaminoheptanedioic acid; L-ornithine; (Nδ-1-(4,4-dimethyl-2,6-dioxo-cyclohex-1-ylidene)ethyl)-D-ornithine; (Nδ-1-(4,4-dimethyl-2,6-dioxo-cyclohex-1-ylidene)ethyl)-L-ornithine; (Nδ-4-methyltrityl)-D-ornithine; (Nδ-4-methyltrityl)-L-ornithine; D-ornithine; L-ornithine; Arg(Me)(Pbf)-OH; Arg(Me)₂-OH (asymmetrical); Arg(Me)2-OH (symmetrical); Lys(ivDde)-OH; Lys(Me)2-OH.HCl; Lys(Me3)-OH chloride; Nω-nitro-D-arginine; and Nω-nitro-L-arginine.
Amino acid analogs include analogs of aspartic or glutamic acids. Examples of amino acid analogs of aspartic and glutamic acids include, but are not limited to, the following: α-methyl-D-aspartic acid; α-methyl-glutamic acid; α-methyl-L-aspartic acid; γ-methylene-glutamic acid; (N-γ-ethyl)-L-glutamine; [N-α-(4-aminobenzoyl)]-L-glutamic acid; 2,6-diaminopimelic acid; L-α-aminosuberic acid; D-2-aminoadipic acid; D-α-aminosuberic acid; α-aminopimelic acid; iminodiacetic acid; L-2-aminoadipic acid; threo-β-methyl-aspartic acid; γ-carboxy-D-glutamic acid γ,γ-di-t-butyl ester; γ-carboxy-L-glutamic acid γ,γ-di-t-butyl ester; Glu(OAll)-OH; L-Asu(OtBu)-OH; and pyroglutamic acid.
Amino acid analogs include analogs of cysteine and methionine. Examples of amino acid analogs of cysteine and methionine include, but are not limited to, Cys(farnesyl)-OH, Cys(farnesyl)-OMe, α-methyl-methionine, Cys(2-hydroxyethyl)-OH, Cys(3-aminopropyl)-OH, 2-amino-4-(ethylthio)butyric acid, buthionine, buthioninesulfoximine, ethionine, methionine methylsulfonium chloride, selenomethionine, cysteic acid, [2-(4-pyridyl)ethyl]-DL-penicillamine, [2-(4-pyridyl)ethyl]-L-cysteine, 4-methoxybenzyl-D-penicillamine, 4-methoxybenzyl-L-penicillamine, 4-methylbenzyl-D-penicillamine, 4-methylbenzyl-L-penicillamine, benzyl-D-cysteine, benzyl-L-cysteine, benzyl-DL-homocysteine, carbamoyl-L-cysteine, carboxyethyl-L-cysteine, carboxymethyl-L-cysteine, diphenylmethyl-L-cysteine, ethyl-L-cysteine, methyl-L-cysteine, t-butyl-D-cysteine, trityl-L-homocysteine, trityl-D-penicillamine, cystathionine, homocystine, L-homocystine, (2-aminoethyl)-L-cysteine, seleno-L-cystine, cystathionine, Cys(StBu)-OH, and acetamidomethyl-D-penicillamine.
Amino acid analogs include analogs of phenylalanine and tyrosine. Examples of amino acid analogs of phenylalanine and tyrosine include β-methyl-phenylalanine, R-hydroxyphenylalanine, α-methyl-3-methoxy-DL-phenylalanine, α-methyl-D-phenylalanine, α-methyl-L-phenylalanine, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid, 2,4-dichloro-phenylalanine, 2-(trifluoromethyl)-D-phenylalanine, 2-(trifluoromethyl)-L-phenylalanine, 2-bromo-D-phenylalanine, 2-bromo-L-phenylalanine, 2-chloro-D-phenylalanine, 2-chloro-L-phenylalanine, 2-cyano-D-phenylalanine, 2-cyano-L-phenylalanine, 2-fluoro-D-phenylalanine, 2-fluoro-L-phenylalanine, 2-methyl-D-phenylalanine, 2-methyl-L-phenylalanine, 2-nitro-D-phenylalanine, 2-nitro-L-phenylalanine, 2;4;5-trihydroxy-phenylalanine, 3,4,5-trifluoro-D-phenylalanine, 3,4,5-trifluoro-L-phenylalanine, 3,4-dichloro-D-phenylalanine, 3,4-dichloro-L-phenylalanine, 3,4-difluoro-D-phenylalanine, 3,4-difluoro-L-phenylalanine, 3,4-dihydroxy-L-phenylalanine, 3,4-dimethoxy-L-phenylalanine, 3,5,3′-triiodo-L-thyronine, 3,5-diiodo-D-tyrosine, 3,5-diiodo-L-tyrosine, 3,5-diiodo-L-thyronine, 3-(trifluoromethyl)-D-phenylalanine, 3-(trifluoromethyl)-L-phenylalanine, 3-amino-L-tyrosine, 3-bromo-D-phenylalanine, 3-bromo-L-phenylalanine, 3-chloro-D-phenylalanine, 3-chloro-L-phenylalanine, 3-chloro-L-tyrosine, 3-cyano-D-phenylalanine, 3-cyano-L-phenylalanine, 3-fluoro-D-phenylalanine, 3-fluoro-L-phenylalanine, 3-fluoro-tyrosine, 3-iodo-D-phenylalanine, 3-iodo-L-phenylalanine, 3-iodo-L-tyrosine, 3-methoxy-L-tyrosine, 3-methyl-D-phenylalanine, 3-methyl-L-phenylalanine, 3-nitro-D-phenylalanine, 3-nitro-L-phenylalanine, 3-nitro-L-tyrosine, 4-(trifluoromethyl)-D-phenylalanine, 4-(trifluoromethyl)-L-phenylalanine, 4-amino-D-phenylalanine, 4-amino-L-phenylalanine, 4-benzoyl-D-phenylalanine, 4-benzoyl-L-phenylalanine, 4-bis(2-chloroethyl)amino-L-phenylalanine, 4-bromo-D-phenylalanine, 4-bromo-L-phenylalanine, 4-chloro-D-phenylalanine, 4-chloro-L-phenylalanine, 4-cyano-D-phenylalanine, 4-cyano-L-phenylalanine, 4-fluoro-D-phenylalanine, 4-fluoro-L-phenylalanine, 4-iodo-D-phenylalanine, 4-iodo-L-phenylalanine, homophenylalanine, thyroxine, 3,3-diphenylalanine, thyronine, ethyl-tyrosine, and methyltyrosine.
Amino acid analogs include analogs of proline. Examples of amino acid analogs of proline include, but are not limited to, 3,4-dehydro-proline, 4-fluoro-proline, cis-4-hydroxy-proline, thiazolidine-2-carboxylic acid, and trans-4-fluoro-proline.
Amino acid analogs include analogs of serine and threonine. Examples of amino acid analogs of serine and threonine include, but are not limited to, 3-amino-2-hydroxy-5-methylhexanoic acid, 2-amino-3-hydroxy-4-methylpentanoic acid, 2-amino-3-ethoxybutanoic acid, 2-amino-3-methoxybutanoic acid, 4-amino-3-hydroxy-6-methylheptanoic acid, 2-amino-3-benzyloxypropionic acid, 2-amino-3-benzyloxypropionic acid, 2-amino-3-ethoxypropionic acid, 4-amino-3-hydroxybutanoic acid, and α-methylserine.
Amino acid analogs include analogs of tryptophan. Examples of amino acid analogs of tryptophan include, but are not limited to, the following: α-methyl-tryptophan; β-(3-benzothienyl)-D-alanine; β-(3-benzothienyl)-L-alanine; 1-methyl-tryptophan; 4-methyl-tryptophan; 5-benzyloxy-tryptophan; 5-bromo-tryptophan; 5-chloro-tryptophan; 5-fluoro-tryptophan; 5-hydroxy-tryptophan; 5-hydroxy-L-tryptophan; 5-methoxy-tryptophan; 5-methoxy-L-tryptophan; 5-methyl-tryptophan; 6-bromo-tryptophan; 6-chloro-D-tryptophan; 6-chloro-tryptophan; 6-fluoro-tryptophan; 6-methyl-tryptophan; 7-benzyloxy-tryptophan; 7-bromo-tryptophan; 7-methyl-tryptophan; D-1,2,3,4-tetrahydro-norharman-3-carboxylic acid; 6-methoxy-1,2,3,4-tetrahydronorharman-1-carboxylic acid; 7-azatryptophan; L-1,2,3,4-tetrahydro-norharman-3-carboxylic acid; 5-methoxy-2-methyl-tryptophan; and 6-chloro-L-tryptophan.
In some embodiments, amino acid analogs are racemic. In some embodiments, the D isomer of the amino acid analog is used. In some embodiments, the L isomer of the amino acid analog is used. In other embodiments, the amino acid analog comprises chiral centers that are in the R or S configuration. In still other embodiments, the amino group(s) of a β-amino acid analog is substituted with a protecting group, e.g., tert-butyloxycarbonyl (BOC group), 9-fluorenylmethyloxycarbonyl (FMOC), tosyl, and the like. In yet other embodiments, the carboxylic acid functional group of a β-amino acid analog is protected, e.g., as its ester derivative. In some embodiments the salt of the amino acid analog is used.
A “non-essential” amino acid residue is a residue that can be altered from the wild-type sequence of a polypeptide without abolishing or substantially altering its essential biological or biochemical activity (e.g., receptor binding or activation). An “essential” amino acid residue is a residue that, when altered from the wild-type sequence of the polypeptide, results in abolishing or substantially abolishing the polypeptide's essential biological or biochemical activity.
A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., K, R, H), acidic side chains (e.g., D, E), uncharged polar side chains (e.g., G, N, Q, S, T, Y, C), nonpolar side chains (e.g., A, V, L, I, P, F, M, W), beta-branched side chains (e.g., T, V, I) and aromatic side chains (e.g., Y, F, W, H). Thus, a predicted nonessential amino acid residue in a polypeptide, for example, is replaced with another amino acid residue from the same side chain family. Other examples of acceptable substitutions are substitutions based on isosteric considerations (e.g. norleucine for methionine) or other properties (e.g. 2-thienylalanine for phenylalanine, or 6-Cl-tryptophan for tryptophan).
The term “capping group” refers to the chemical moiety occurring at either the carboxy or amino terminus of the polypeptide chain of the subject peptidomimetic macrocycle. The capping group of a carboxy terminus includes an unmodified carboxylic acid (ie —COOH) or a carboxylic acid with a substituent. For example, the carboxy terminus can be substituted with an amino group to yield a carboxamide at the C-terminus. Various substituents include but are not limited to primary and secondary amines, including pegylated secondary amines. Representative secondary amine capping groups for the C-terminus include:
The capping group of an amino terminus includes an unmodified amine (ie —NH₂) or an amine with a substituent. For example, the amino terminus can be substituted with an acyl group to yield a carboxamide at the N-terminus. Various substituents include but are not limited to substituted acyl groups, including C₁-C₆carbonyls, C₇-C₃₀carbonyls, and pegylated carbamates. Representative capping groups for the N-terminus include, but are not limited to, 4-FBzl (4-fluoro-benzyl) and the following:
The term “member” as used herein in conjunction with macrocycles or macrocycle-forming linkers refers to the atoms that form or can form the macrocycle, and excludes substituent or side chain atoms. By analogy, cyclodecane, 1,2-difluoro-decane and 1,3-dimethyl cyclodecane are all considered ten-membered macrocycles as the hydrogen or fluoro substituents or methyl side chains do not participate in forming the macrocycle.
The symbol “
” when used as part of a molecular structure refers to a single bond or a trans or cis double bond.
The term “amino acid side chain” refers to a moiety attached to the α-carbon (or another backbone atom) in an amino acid. For example, the amino acid side chain for alanine is methyl, the amino acid side chain for phenylalanine is phenylmethyl, the amino acid side chain for cysteine is thiomethyl, the amino acid side chain for aspartate is carboxymethyl, the amino acid side chain for tyrosine is 4-hydroxyphenylmethyl, etc. Other non-naturally occurring amino acid side chains are also included, for example, those that occur in nature (e.g., an amino acid metabolite) or those that are made synthetically (e.g., an α,α di-substituted amino acid).
The term “α,α di-substituted amino” acid refers to a molecule or moiety containing both an amino group and a carboxyl group bound to a carbon (the α-carbon) that is attached to two natural or non-natural amino acid side chains.
The term “polypeptide” encompasses two or more naturally or non-naturally-occurring amino acids joined by a covalent bond (e.g., an amide bond). Polypeptides as described herein include full length proteins (e.g., fully processed proteins) as well as shorter amino acid sequences (e.g., fragments of naturally-occurring proteins or synthetic polypeptide fragments).
The term “first C-terminal amino acid” refers to the amino acid which is closest to the C-terminus. The term “second C-terminal amino acid” refers to the amino acid attached at the N-terminus of the first C-terminal amino acid.
The term “macrocyclization reagent” or “macrocycle-forming reagent” as used herein refers to any reagent which can be used to prepare a peptidomimetic macrocycle by mediating the reaction between two reactive groups. Reactive groups can be, for example, an azide and alkyne, in which case macrocyclization reagents include, without limitation, Cu reagents such as reagents which provide a reactive Cu(I) species, such as CuBr, CuI or CuOTf, as well as Cu(II) salts such as Cu(CO₂CH₃)₂, CuSO₄, and CuCl₂that can be converted in situ to an active Cu(I) reagent by the addition of a reducing agent such as ascorbic acid or sodium ascorbate.
Macrocyclization reagents can additionally include, for example, Ru reagents known in the art such as Cp*RuCl(PPh₃)₂, [Cp*RuCl]₄or other Ru reagents which can provide a reactive Ru(II) species. In other cases, the reactive groups are terminal olefins. In such embodiments, the macrocyclization reagents or macrocycle-forming reagents are metathesis catalysts including, but not limited to, stabilized, late transition metal carbene complex catalysts such as Group VIII transition metal carbene catalysts. For example, such catalysts are Ru and Os metal centers having a +2 oxidation state, an electron count of 16 and pentacoordinated. In other examples, catalysts have W or Mo centers. Various catalysts are disclosed in Grubbs et al., “Ring Closing Metathesis and Related Processes in Organic Synthesis” Acc. Chem. Res. 1995, 28, 446-452, U.S. Pat. Nos. 5,811,515; 7,932,397; U.S. Application No. 2011/0065915; U.S. Application No. 2011/0245477; Yu et al., “Synthesis of Macrocyclic Natural Products by Catalyst-Controlled Stereoselective Ring-Closing Metathesis,” Nature 2011, 479, 88; and Peryshkov et al., “Z-Selective Olefin Metathesis Reactions Promoted by Tungsten Oxo Alkylidene Complexes,” J. Am. Chem. Soc. 2011, 133, 20754. In yet other cases, the reactive groups are thiol groups. In such embodiments, the macrocyclization reagent is, for example, a linker functionalized with two thiol-reactive groups such as halogen groups. In some examples, the macrocyclization reagent include palladium reagents, for example Pd(PPh₃)₄, Pd(PPh₃)₂Cl₂, Pd(dppe)Cl, Pd(dppp)Cl₂, and Pd(dppf)Cl₂. The term “halo” or “halogen” refers to fluorine, chlorine, bromine or iodine or a radical thereof.
The term “alkyl” refers to a hydrocarbon chain that is a straight chain or branched chain, containing the indicated number of carbon atoms. For example, C₁-C₁₀indicates that the group has from 1 to 10 (inclusive) carbon atoms in it. In the absence of any numerical designation, “alkyl” is a chain (straight or branched) having 1 to 20 (inclusive) carbon atoms in it.
The term “alkylene” refers to a divalent alkyl (i.e., —R—).
The term “alkenyl” refers to a hydrocarbon chain that is a straight chain or branched chain having one or more carbon-carbon double bonds. The alkenyl moiety contains the indicated number of carbon atoms. For example, C₂-C₁₀indicates that the group has from 2 to 10 (inclusive) carbon atoms in it. The term “lower alkenyl” refers to a C₂-C₆alkenyl chain. In the absence of any numerical designation, “alkenyl” is a chain (straight or branched) having 2 to 20 (inclusive) carbon atoms in it.
The term “alkynyl” refers to a hydrocarbon chain that is a straight chain or branched chain having one or more carbon-carbon triple bonds. The alkynyl moiety contains the indicated number of carbon atoms. For example, C₂-C₁₀indicates that the group has from 2 to 10 (inclusive) carbon atoms in it. The term “lower alkynyl” refers to a C₂-C₆alkynyl chain. In the absence of any numerical designation, “alkynyl” is a chain (straight or branched) having 2 to 20 (inclusive) carbon atoms in it.
The term “aryl” refers to a 6-carbon monocyclic or 10-carbon bicyclic aromatic ring system wherein 0, 1, 2, 3, or 4 atoms of each ring are substituted by a substituent. Examples of aryl groups include phenyl, naphthyl and the like. The term “arylalkoxy” refers to an alkoxy substituted with aryl.
“Arylalkyl” refers to an aryl group, as defined above, wherein one of the aryl group's hydrogen atoms has been replaced with a C₁-C₅alkyl group, as defined above. Representative examples of an arylalkyl group include, but are not limited to, 2-methylphenyl, 3-methylphenyl, 4-methylphenyl, 2-ethylphenyl, 3-ethylphenyl, 4-ethylphenyl, 2-propylphenyl, 3-propylphenyl, 4-propylphenyl, 2-butylphenyl, 3-butylphenyl, 4-butylphenyl, 2-pentylphenyl, 3-pentylphenyl, 4-pentylphenyl, 2-isopropylphenyl, 3-isopropylphenyl, 4-isopropylphenyl, 2-isobutylphenyl, 3-isobutylphenyl, 4-isobutylphenyl, 2-sec-butylphenyl, 3-sec-butylphenyl, 4-sec-butylphenyl, 2-t-butylphenyl, 3-t-butylphenyl and 4-t-butylphenyl.
“Arylamido” refers to an aryl group, as defined above, wherein one of the aryl group's hydrogen atoms has been replaced with one or more —C(O)NH₂groups. Representative examples of an arylamido group include 2-C(O)NH2-phenyl, 3-C(O)NH₂-phenyl, 4-C(O)NH₂-phenyl, 2-C(O)NH₂-pyridyl, 3-C(O)NH₂-pyridyl, and 4-C(O)NH₂-pyridyl,
“Alkylheterocycle” refers to a C₁-C₅alkyl group, as defined above, wherein one of the C₁-C₅alkyl group's hydrogen atoms has been replaced with a heterocycle. Representative examples of an alkylheterocycle group include, but are not limited to, —CH₂CH₂-morpholine, —CH₂CH₂-piperidine, —CH₂CH₂CH₂-morpholine, and —CH₂CH₂CH₂-imidazole.
“Alkylamido” refers to a C₁-C₅alkyl group, as defined above, wherein one of the C₁-C₅alkyl group's hydrogen atoms has been replaced with a —C(O)NH₂group. Representative examples of an alkylamido group include, but are not limited to, —CH₂—C(O)NH₂, —CH₂CH₂—C(O)NH₂, —CH₂CH₂CH₂C(O)NH₂, —CH₂CH₂CH₂CH₂C(O)NH₂, —CH₂CH₂CH₂CH₂CH₂C(O)NH₂, —CH₂CH(C(O)NH₂)CH₃, —CH₂CH(C(O)NH₂)CH₂CH₃, —CH(C(O)NH₂)CH₂CH₃, —C(CH₃)₂CH₂C(O)NH₂, —CH₂—CH₂—NH—C(O)—CH₃, —CH₂—CH₂—NH—C(O)—CH₃—CH3, and —CH₂—CH₂—NH—C(O)—CH═CH₂.
“Alkanol” refers to a C₁-C₅alkyl group, as defined above, wherein one of the C₁-C₅alkyl group's hydrogen atoms has been replaced with a hydroxyl group. Representative examples of an alkanol group include, but are not limited to, —CH₂OH, —CH₂CH₂OH, —CH₂CH₂CH₂OH, —CH₂CH₂CH₂CH₂OH, —CH₂CH₂CH₂CH₂CH₂OH, —CH₂CH(OH)CH₃, —CH₂CH(OH)CH₂CH₃, —CH(OH)CH₃and —C(CH₃)₂CH₂OH.
“Alkylcarboxy” refers to a C₁-C₅alkyl group, as defined above, wherein one of the C₁-C₅alkyl group's hydrogen atoms has been replaced with a —COOH group. Representative examples of an alkylcarboxy group include, but are not limited to, —CH₂COOH, —CH₂CH₂COOH, —CH₂CH₂CH₂COOH, —CH₂CH₂CH₂CH₂COOH, —CH₂CH(COOH)CH₃, —CH₂CH₂CH₂CH₂CH₂COOH, —CH₂CH(COOH)CH₂CH₃, —CH(COOH)CH₂CH₃and —C(CH₃)₂CH₂COOH.
The term “cycloalkyl” as employed herein includes saturated and partially unsaturated cyclic hydrocarbon groups having 3 to 12 carbons, preferably 3 to 8 carbons, and more preferably 3 to 6 carbons, wherein the cycloalkyl group additionally is optionally substituted. Some cycloalkyl groups include, without limitation, cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, and cyclooctyl.
The term “heteroaryl” refers to an aromatic 5-8 membered monocyclic, 8-12 membered bicyclic, or 11-14 membered tricyclic ring system having 1-3 heteroatoms if monocyclic, 1-6 heteroatoms if bicyclic, or 1-9 heteroatoms if tricyclic, said heteroatoms selected from O, N, or S (e.g., carbon atoms and 1-3, 1-6, or 1-9 heteroatoms of O, N, or S if monocyclic, bicyclic, or tricyclic, respectively), wherein 0, 1, 2, 3, or 4 atoms of each ring are substituted by a substituent. Examples of heteroaryl groups include pyridyl, furyl or furanyl, imidazolyl, benzimidazolyl, pyrimidinyl, thiophenyl or thienyl, quinolinyl, indolyl, thiazolyl, and the like.
The term “heteroarylalkyl” or the term “heteroaralkyl” refers to an alkyl substituted with a heteroaryl. The term “heteroarylalkoxy” refers to an alkoxy substituted with heteroaryl.
The term “heteroarylalkyl” or the term “heteroaralkyl” refers to an alkyl substituted with a heteroaryl. The term “heteroarylalkoxy” refers to an alkoxy substituted with heteroaryl.
The term “heterocyclyl” refers to a nonaromatic 5-8 membered monocyclic, 8-12 membered bicyclic, or 11-14 membered tricyclic ring system having 1-3 heteroatoms if monocyclic, 1-6 heteroatoms if bicyclic, or 1-9 heteroatoms if tricyclic, said heteroatoms selected from O, N, or S (e.g., carbon atoms and 1-3, 1-6, or 1-9 heteroatoms of O, N, or S if monocyclic, bicyclic, or tricyclic, respectively), wherein 0, 1, 2 or 3 atoms of each ring are substituted by a substituent. Examples of heterocyclyl groups include piperazinyl, pyrrolidinyl, dioxanyl, morpholinyl, tetrahydrofuranyl, and the like.
The term “substituent” refers to a group replacing a second atom or group such as a hydrogen atom on any molecule, compound or moiety. Suitable substituents include, without limitation, halo, hydroxy, mercapto, oxo, nitro, haloalkyl, alkyl, alkaryl, aryl, aralkyl, alkoxy, thioalkoxy, aryloxy, amino, alkoxycarbonyl, amido, carboxy, alkanesulfonyl, alkylcarbonyl, and cyano groups.
In some embodiments, the compounds disclosed herein contain one or more asymmetric centers and thus occur as racemates and racemic mixtures, single enantiomers, individual diastereomers and diastereomeric mixtures. All such isomeric forms of these compounds are included unless expressly provided otherwise. In some embodiments, the compounds disclosed herein are also represented in multiple tautomeric forms, in such instances, the compounds include all tautomeric forms of the compounds described herein (e.g., if alkylation of a ring system results in alkylation at multiple sites, the disclosure includes all such reaction products). All such isomeric forms of such compounds are included unless expressly provided otherwise. All crystal forms of the compounds described herein are included unless expressly provided otherwise.
As used herein, the terms “increase” and “decrease” mean, respectively, to cause a statistically significantly (i.e., p<0.1) increase or decrease of at least 5%.
As used herein, the recitation of a numerical range for a variable is intended to convey that the variable is equal to any of the values within that range. Thus, for a variable which is inherently discrete, the variable is equal to any integer value within the numerical range, including the end-points of the range. Similarly, for a variable which is inherently continuous, the variable is equal to any real value within the numerical range, including the end-points of the range. As an example, and without limitation, a variable which is described as having values between 0 and 2 takes the values 0, 1 or 2 if the variable is inherently discrete, and takes the values 0.0, 0.1, 0.01, 0.001, or any other real values ≥0 and ≤2 if the variable is inherently continuous.
As used herein, unless specifically indicated otherwise, the word “or” is used in the inclusive sense of “and/or” and not the exclusive sense of “either/or.”
The term “on average” represents the mean value derived from performing at least three independent replicates for each data point.
The term “biological activity” encompasses structural and functional properties of a macrocycle.
Biological activity is, for example, structural stability, alpha-helicity, affinity for a target, resistance to proteolytic degradation, cell penetrability, intracellular stability, in vivo stability, or any combination thereof.
The term “binding affinity” refers to the strength of a binding interaction, for example between a peptidomimetic macrocycle and a target. Binding affinity can be expressed, for example, as an equilibrium dissociation constant (“K_D”), which is expressed in units which are a measure of concentration (e.g. M, mM, M, nM etc). Numerically, binding affinity and K_Dvalues vary inversely, such that a lower binding affinity corresponds to a higher K_Dvalue, while a higher binding affinity corresponds to a lower K_Dvalue. Where high binding affinity is desirable, “improved” binding affinity refers to higher binding affinity and therefore lower K_Dvalues.
The term “in vitro efficacy” refers to the extent to which a test compound, such as a peptidomimetic macrocycle, produces a beneficial result in an in vitro test system or assay. In vitro efficacy can be measured, for example, as an “IC₅₀” or “EC₅₀” value, which represents the concentration of the test compound which produces 50% of the maximal effect in the test system.
The term “ratio of in vitro efficacies” or “in vitro efficacy ratio” refers to the ratio of IC₅₀or EC₅₀values from a first assay (the numerator) versus a second assay (the denominator). Consequently, an improved in vitro efficacy ratio for Assay 1 versus Assay 2 refers to a lower value for the ratio expressed as IC₅₀(Assay 1)/IC₅₀(Assay 2) or alternatively as EC₅₀(Assay 1)/EC₅₀(Assay 2). This concept can also be characterized as “improved selectivity” in Assay 1 versus Assay 2, which can be due either to a decrease in the IC₅₀or EC₅₀value for Target 1 or an increase in the value for the IC₅₀or EC₅₀value for Target 2.
“Micelle forming agent” as used herein can be an amphiphilic compound meaning a compound that contains both hydrophobic groups (tails) and hydrophilic groups (heads). Micelle forming agents include surfactant, for examples ionic, non-ionic, and zwitterionic surfactants.
The details of one or more particular embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims.
The term “Xaa” is used in the Formulas described herein to refer to any amino acids. This term can sometimes be followed by a number subscript, for e.g. “Xaa₆.” The number subscript in these cases may or may not refer to the position of the amino acids “Xaa” in a sequence. For example in some but not all cases Xaa₆can mean that the amino acid “Xaa” is present at the sixth position in a sequence.

Overview

In one aspect the disclosure provides aqueous pharmaceutical formulations, for parenteral administration, comprising peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof, wherein the peptidomimetic macrocycle binds to MDM2 and/or MDMX proteins. The aqueous pharmaceutical formulations provided herein are aqueous solution ready for injection (for example intravenously) or aqueous concentrations ready for dilution and injection. In some embodiments, the aqueous pharmaceutical formulations disclosed herein do not contain micelles or are essentially free of micelles. In various embodiments, the aqueous pharmaceutical formulations disclosed herein comprise less than 2% w/v of a micelle forming agent. In some examples the aqueous pharmaceutical formulations disclosed herein comprise less than 2%, 1.9%, 1.8%, 1.7%, 1.6%, 1.5%, 1.4%, 1.3%, 1.2%, 1.1%, 1.0%, 0.9%, 08%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3%, 0.2%, 0.1%, 0.09%, 0.08%, 0.07%, 0.06%, or 0.05% w/v of a micelle forming agent. In some embodiments, the micelle forming agent is sorbitol. In some embodiments, the micelle forming agent is polyethylene glycol-poly(lactic acid). In some embodiments, the micelle forming agent is 1,2-distearoyl-phosphatidylethanolamine-methyl-polyethyleneglycol conjugate. In some embodiments, no micelle forming agent is used, yet the molecule has micelle forming properties.
The aqueous pharmaceutical formulations comprise an aqueous diluent. In some examples, the diluent is water, purified water, water for injection, bacteriostatic water for injection, sterile water for injection, water for parenterals, PBS, and/or, sterile water for irrigation. In some embodiments, the diluent is water for injection. In some embodiments, the diluent is PBS. In some embodiments, the diluent is a solution of dextrose in water, for example 5% dextrose in water.
In various embodiments, the peptidomimetic macrocycle is a cross-linked peptide comprising at least one macrocycle-forming linker which forms a macrocycle between a first amino acid residue (or analog) and a second amino acid residue. In some embodiments, a peptidomimetic macrocycle has the Formula (I):
wherein:
each A, C, and D is independently an amino acid;
each B is independently an amino acid,
[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];
each E is independently an amino acid selected from the group consisting of Ala (alanine), D-Ala (D-alanine), Aib (α-aminoisobutyric acid), Sar (N-methyl glycine), and Ser (serine);
each R₁and R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo; or forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids;
each R₃is independently hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
each L and L′ is independently a macrocycle-forming linker;
each L₃is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
each v is independently an integer from 1-1000;
each w is independently an integer from 3-1000;
u is an integer from 1-10;
each x, y and z is independently an integer from 0-10; and
each n is independently an integer from 1-5.
In some embodiments, the macrocycle-forming linker (L or L′) has a formula -L₁-L₂-, wherein L₁and L₂are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃; and
each R₃independently is hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
each n is an integer from 1-5.
In some embodiments the peptidomimetic macrocycle is a p53-based peptidomimetic macrocycle capable of binding to and modulating the activity of p53, MDM2 and/or MDMX. In some embodiments the peptidomimetic macrocycle is a p53-based peptidomimetic macrocycle that inhibits the interactions between p53, MDM2 and/or MDMX proteins. In some embodiments the peptidomimetic macrocycle is a p53-based peptidomimetic macrocycle that can be used for treating diseases including but not limited to cancer and other hyperproliferative diseases. In some examples, the peptidomimetic macrocycle has a Formula I and comprises an amino acid sequence which is at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or more identical to an amino acid sequence in any of Table 1, Table 1a, Table 1b, and Table 1c. In some examples, the peptidomimetic macrocycle in a peptidomimetic macrocycle from the any of Table 1, Table 1a, Table 1b, and Table 1c.
Any suitable dosage of peptidomimetic macrocycles can be formulated in the aqueous pharmaceutical formulations of the present disclosure. Generally, the peptidomimetic macrocycle (or, in embodiments comprising two or more peptidomimetic macrocycles, each of the peptidomimetic macrocycle) is present in the aqueous pharmaceutical formulation in an amount greater than or equal to 1 mg/mL. For example greater than or equal to 5 mg/mL, 10 mg/mL, 15 mg/mL, 20 mg/mL, 30 mg/mL, 40 mg/mL, and 50 mg/mL. In some examples, the peptidomimetic macrocycle is present in the aqueous pharmaceutical formulation in an amount ranging from about 15 mg/mL to about 100 mg/mL. In some embodiments, the peptidomimetic macrocycle is present in the aqueous pharmaceutical formulation in an amount ranging from about 15 mg/mL to about 60 mg/mL. In some embodiments, the peptidomimetic macrocycle is present in the aqueous pharmaceutical formulation in an amount ranging from about 20 mg/mL to about 50 mg/mL. In some embodiments, the peptidomimetic macrocycle is present in the aqueous pharmaceutical formulation in an amount ranging from about 50 mg/mL to about 100 mg/mL. In some embodiments, the peptidomimetic macrocycles is present in the aqueous pharmaceutical formulation in an amount ranging from about 15 mg/mL to about 20 mg/mL. In some embodiments, the peptidomimetic macrocycles is present in the aqueous pharmaceutical formulation in an amount ranging from about 15 mg/mL to about 30 mg/mL. It will be readily apparent to those of skill that the peptidomimetic macrocycle dosage can be varied depending on several conditions including the peptidomimetic macrocycle used, the subject to be treated, and the disease, disorder or condition to be treated.
The aqueous pharmaceutical formulations disclosed herein can additionally comprise a buffering agent. The buffering agent can be any agent capable of maintaining the pH of the aqueous formulation in the range of 4.0-9.0. For example, the buffering agent is selected from a group consisting of ammonia solution, calcium carbonate, tribasic calcium phosphate, citric acid monohydrate, dibasic sodium phosphate, diethanolamine, malic acid, monobasic sodium phosphate, monoethanolamine, monosodium glutamate, phosphoric acid, potassium citrate, sodium acetate, sodium bicarbonate, sodium borate, sodium citrate dehydrate, sodium hydroxide, sodium lactate and triethanolamine. In some embodiments, the buffering agent can be monobasic sodium phosphate, dibasic sodium phosphate, or a mixture thereof. The pH of the formulation can be in the range of 4.0-9.0. For example, the pH can be in the range of about 4.5-8.5, about 5.0-8.0, about 5.5-7.5, about 7.0-7.5, about 7.0-8.0, about 7.0-9.0, or about 8.0-9.0. In some embodiments, the pH of the formulations is about 7.0. In some embodiments, the pH of the formulations is about 7.5. In some embodiments, the pH of the formulations is about 8.0.
The aqueous pharmaceutical formulations disclosed herein can comprise a stabilizing agent. The stabilizing agent can be any pharmaceutically acceptable stabilizing agent. Such stabilizing agent can include, for example antioxidants and/or surfactants. In some embodiments, the stabilizing agent is a non-ionic stabilizing agent, for example as non-ionic surfactant. In some embodiments, the stabilizing agent is a fatty acid ester. The stabilizing agent can be selected from a group consisting of polyoxyethylene glycol alkyl ethers, polyoxypropylene glycol alkyl ethers, glucoside alkyl ethers, polyoxyethylene glycol octylphenol ethers, polyoxyethylene glycol alkylphenol ethers, glycerol alkyl esters, polyoxyethylene glycol sorbitan alkyl esters, sorbitan alkyl esters, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide, block copolymers of polyethylene glycol and polypropylene glycol, and polyethoxylated tallow amine. In some examples, the stabilizing agent is a polyoxyethylene sorbitan fatty acid ester, for example polysorbate 20, polysorbate 21, polysorbate 40, polysorbate 60, polysorbate 61, polysorbate 65, polysorbate 80, polysorbate 81, polysorbate 85 or polysorbate 120.
In some embodiments, the tonicity of the instant aqueous pharmaceutical formulations can be adjusted, for example the tonicity of the formulations can be such that the formulations are isotonic with the physiologic fluid. Such formulations can further comprise one or more tonicity adjusting agent (tonicity agent) to adjust the tonicity of the formulations. Any pharmaceutically acceptable tonicity agent can be used. In some examples the tonicity agents are selected from a group consisting of electrolytes, monosaccharides, disaccharides, polysaccharides, and water-soluble glucans. In some examples the tonicity agent in NaCl or KCl. In some examples the tonicity agent is selected from a group consisting of fructose, glucose, mannose, mannitol, sorbose, xylose, maltose, lactose, sucrose, trehalose, dextran, pullulan, dextrin, cyclodextrin, soluble starch, hydroxyethyl starch and carboxymethylcellulose. In some embodiments, the tonicity agent is trehalose.
In some examples, the formulations of the present disclosure further comprise one or more additional excipients. For example a preservative or a co-solvent.
Also provided herein are methods of making the aqueous pharmaceutical formulations disclosed herein. The method comprises adding a peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof to an aqueous solution. The aqueous solution can comprise one or more of a buffering agent, a stabilizing agent, and a tonicity agent. The method can further comprise adding a pH adjusting agent to maintain the pH of the mixture at a specified level. In some embodiments, the method comprises adding a desired amount of the peptidomimetic macrocycle or a pharmaceutically acceptable salt (for example sodium, potassium or lithium salt) thereof to water. In some embodiments, the method comprises adding a desired amount of the peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof to an aqueous solution comprising a buffering agent, a stabilizing agent, and a tonicity agent.
Also provided herein is a method for treating a disease, condition or disorder that can be treated, alleviated, or prevented by administering to a subject an aqueous pharmaceutical formulation as described herein. The method comprises, administering to the subject the aqueous pharmaceutical formulation in an amount effective to treat, alleviate or prevent the disease, condition, or disorder. In some embodiments, the disease, condition, or disorder is a p53 mediated disease, condition, or disorder. In some embodiments, the disease, condition, or disorder is a MDM2 and/or MDMX mediated disease, condition, or disorder. In some embodiments, the disease, condition, or disorder is a hyperproliferative disease and/or an inflammatory disorder. In some embodiments, the disease, condition, or disorder is cancers and neoplastic conditions. In some examples, the cancer is selected from a group consisting of pancreatic cancer, bladder cancer, colon cancer, liver cancer, colorectal cancer, breast cancer, prostate cancer, renal cancer, hepatocellular cancer, lung cancer, ovarian cancer, cervical cancer, gastric cancer, esophageal cancer, head and neck cancer, melanoma, neuroendocrine cancers, CNS cancers, brain tumors, bone cancer, skin cancer, ocular tumor, rectal cancer, choriocarcinoma (tumor of the placenta), sarcoma and soft tissue cancer, testicular cancer, gall bladder cancer, and biliary cancer. In some examples, the cancer is selected from a group consisting of bladder cancer, bone cancer, breast cancer, cervical cancer, CNS cancer, colon cancer, ocular tumor, renal cancer, liver cancer, lung cancer, pancreatic cancer, choriocarcinoma (tumor of the placenta), prostate cancer, sarcoma, skin cancer, soft tissue cancer, gastric cancer, gall bladder cancer, biliary cancer, renal cancer, neoblastoma, or neuroendocrine cancer. Non-limiting examples of ocular tumor include choroidal nevus, choroidal melanoma, choroidal metastasis, choroidal hemangioma, choroidal osteoma, iris melanoma, uveal melanoma, melanocytoma, metastasis retinal capillary hemangiomas, congenital hypertrophy of the RPE, RPE adenoma or retinoblastoma. In some cases, the cancer is selected from non-small cell lung cancer, small-cell lung cancer, colon cancer, CNS cancer, melanoma, ovarian cancer, renal cancer, prostate cancer and breast cancer. In some examples, the cancer is breast cancer. In some examples, the cancer is gall bladder cancer. In some examples, the cancer is biliary cancer. In some examples, the cancer is neuroendocrine cancer. In some examples, the cancer is bone cancer. In some examples, the cancer is the bone cancer is osteosarcoma. In some examples, the cancer is skin cancer. In some examples, the cancer is melanoma.
In another aspect, the present disclosure provides kits for treating a disease, condition or disorder, wherein the kit comprises the aqueous pharmaceutical disclosed herein. The formulations can be packaged in any suitable container, for example a bottle or a vial. In some examples, the formulations can be packed in glass serum vial. In some examples, the formulations can be packed in serum vials composed of borosilicate glass. In some examples, the formulations are packed in a 1 mL, a 2 mL, a 3 mL, a 4 mL, a 5 mL, a 10 mL, a 20 mL, a 30 mL, or a 50 mL glass vial. The bottles and/or vials can be equipped with stoppers and/or seals. For example, the formulations can be packaged into glass vials equipped with Teflon stoppers and/or a flip-off cap. The flip-off cap can be a plastic cap. The glass container can be an ampoule. The formulations can be packaged in multidose form or in single dose form. In some cases, the formulations are packaged in multidose forms. In some embodiments the formulations are packaged as single dose units. In some embodiments, the kit further comprises instructions, wherein the instructions direct the administration of the formulation to treat the subject in need thereof. The kit can also include a device for administration of the formulation.

Aqueous Pharmaceutical Formulations of Peptidomimetic Macrocycles for Parenteral Administration

In one aspect, the disclosure provides aqueous pharmaceutical formulations, suitable for parenteral administration, comprising peptidomimetic macrocycles, as described herein and an aqueous diluent. The aqueous pharmaceutical formulations provided herein can be suitable for intravenous, intra-arterial, intrathecal, or subcutaneous administration. In some embodiments, the aqueous pharmaceutical formulations are suitable for intravenous administration. The aqueous pharmaceutical formulations described herein can provide improved solubility and/or stability of the peptidomimetic macrocycle. In particular embodiments, the aqueous pharmaceutical formulations provide increased solubility of the peptidomimetic macrocycles compared to the solubility of the peptidomimetic macrocycles peptide in water alone.
In some examples, the aqueous diluent is water, purified water, water for injection, bacteriostatic water for injection, sterile water for injection, water for parenterals, sterile water for irrigation, various sterile solution of electrolytes and or dextrose. In some embodiments, the diluent is a pH buffered solution (e.g. phosphate-buffered saline), sterile saline solution, Ringer's solution or dextrose solution. In some embodiments, the diluent is water for injection. In some embodiments, the diluent is a solution of dextrose in water, for example 5% dextrose in water.
The aqueous pharmaceutical formulations my further comprise a co-solvent. A co-solvent is any solvent that facilitates/enhances the solubility of the peptidomimetic macrocycles (or of the one or more excipients) in the aqueous diluent. The co-solvent is preferably water miscible. In some embodiments, the co-solvent is ethyl alcohol, glycerin, polyethylene glycol, or propylene glycol. In some embodiments, the co-solvent is dimethyl sulfoxide (DMSO), N-methylpyrrolidone (NMP), dimethylacetamide (DMA) or a combination thereof.
The aqueous pharmaceutical formulations provided herein are aqueous solution ready for injection (for example intravenously) or aqueous concentrations ready for dilution and injection. In some embodiments, the aqueous pharmaceutical formulations disclosed herein do not contain micelles or are essentially free of micelles. In various embodiments, the aqueous pharmaceutical formulations disclosed herein comprise less than 2% w/v of a micelle forming agent. In some examples the aqueous pharmaceutical formulations disclosed herein comprise less than 2%, 1.9%, 1.8%, 1.7%, 1.6%, 1.5%, 1.4%, 1.3%, 1.2%, 1.1%, 1.0%, 0.9%, 08%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3%, 0.2%, 0.1%, 0.09%, 0.08%, 0.07%, 0.06%, or 0.05% w/v of a micelle forming agent. In some examples the aqueous pharmaceutical formulations disclosed herein comprise 0.0001%-2%, 0.0005%-2%, 0.001%-2%, 0.005%-2%, 0.01%-2%, 0.05%-2%, 0.1%-2%, 0.2%-2%, 0.3%-2%, 0.4%-2%, 0.5%-2%, 0.6%-2%, 0.7%-2%, 0.8%-2%, 0.9%-2%, 1.0%-2%, 1.1%-2%, 1.2%-2%, 1.3%-2%, 1.4%-2%, 1.5%-2%, 1.6%-2%, 1.7%-2%, 1.8%-2%, 1.9%-2%, 0.0001%-1.8%, 0.0005%-1.8%, 0.001%-1.8%, 0.005%-1.8%, 0.01%-1.8%, 0.05%-1.8%, 0.1%-1.8%, 0.2%-1.8%, 0.3%-1.8%, 0.4%-1.8%, 0.5%-1.8%, 0.6%-1.8%, 0.7%-1.8%, 0.8%-1.8%, 0.9%-1.8%, 1.0%-1.8%, 1.1%-1.8%, 1.2%-1.8%, 1.3%-1.8%, 1.4%-1.8%, 1.5%-1.8%, 1.6%-1.8%, 0.0001%-1.6%, 0.0005%-1.6%, 0.001%-1.6%, 0.005%-1.6%, 0.01%-1.6%, 0.05%-1.6%, 0.1%-1.6%, 0.2%-1.6%, 0.3%-1.6%, 0.4%-1.6%, 0.5%-1.6%, 0.6%-1.6%, 0.7%-1.6%, 0.8%-1.6%, 0.9%-1.6%, 1.0%-1.6%, 1.1%-1.6%, 1.2%-1.6%, 1.3%-1.6%, 1.4%-1.6%, 1.5%-1.6%, 0.0001%-1.4%, 0.0005%-1.4%, 0.001%-1.4%, 0.005%-1.4%, 0.01%-1.4%, 0.05%-1.4%, 0.1%-1.4%, 0.2%-1.4%, 0.3%-1.4%, 0.4%-1.4%, 0.5%-1.4%, 0.6%-1.4%, 0.7%-1.4%, 0.8%-1.4%, 0.9%-1.4%, 1.0%-1.4%, 1.1%-1.4%, 1.2%-1.4%, 1.3%-1.4%, 0.0001%-1.2%, 0.0005%-1.2%, 0.001%-1.2%, 0.005%-1.2%, 0.01%-1.2%, 0.05%-1.2%, 0.1%-1.2%, 0.2%-1.2%, 0.3%-1.2%, 0.4%-1.2%, 0.5%-1.2%, 0.6%-1.2%, 0.7%-1.2%, 0.8%-1.2%, 0.9%-1.2%, 1.0%-1.2%, 1.1%-1.2%, 0.0001%-1%, 0.0005%-1%, 0.001%-1%, 0.005%-1%, 0.01%-1%, 0.05%-1%, 0.1%-1%, 0.2%-1%, 0.3%-1%, 0.4%-1%, 0.5%-1%, 0.6%-1%, 0.7%-1%, 0.0001%-0.8%, 0.0005%-0.8%, 0.001%-0.8%, 0.005%-0.8%, 0.01%-0.8%, 0.05%-0.8%, 0.1%-0.8%, 0.2%-0.8%, 0.3%-0.8%, 0.4%-0.8%, 0.5%-0.8%, 0.6%-0.8%, 0.7%-0.8%, 0.0001%-0.6%, 0.0005%-0.6%, 0.001%-0.6%, 0.005%-0.6%, 0.01%-0.6%, 0.05%-0.6%, 0.1%-0.6%, 0.2%-0.6%, 0.3%-0.6%, 0.4%-0.6%, 0.5%-0.6%, 0.0001%-0.4%, 0.0005%-0.4%, 0.001%-0.4%, 0.005%-0.4%, 0.01%-0.4%, 0.05%-0.4%, 0.1%-0.4%, 0.2%-0.4%, 0.3%-0.4%, 0.0001%-0.2%, 0.0005%-0.2%, 0.001%-0.2%, 0.005%-0.2%, 0.01%-0.2%, 0.05%-0.2%, 0.1%-0.2%, 0.0001%-0.1%, 0.0005%-0.1%, 0.001%-0.1%, 0.005%-0.1%, 0.01%-0.1%, 0.05%-0.1, 0.0001%-0.05%, 0.0005%-0.05%, 0.001%-0.05%, 0.005%-0.05%, 0.01%-0.05%, 0.0001%-0.01%, 0.0005%-0.01%, 0.001%-0.01%, 0.005%-0.01%, 0.0001%-0.005%, 0.0005%-0.005%, 0.001%-0.005%, 0.0001%-0.001%, 0.0005%-0.001%, or 0.0001%-0.0005% w/v of a micelle forming agent. In some embodiments, the micelle forming agent is sorbitol. In some embodiments, the micelle forming agent is Polyethylene glycol-Poly(lactic acid). In some embodiments, the micelle forming agent is 1,2-Distearoyl-phosphatidylethanolamine-methyl-polyethyleneglycol conjugate. In some embodiments, no micelle-forming agent is added in the formulation, but the molecule has micelle-forming properties.
The aqueous pharmaceutical formulations disclosed herein can additionally comprise one or more excipients suitable for aqueous pharmaceutical formulations. Exemplary excipients that can be present in the aqueous pharmaceutical formulations described herein are described below.

Buffering Agents

The aqueous pharmaceutical formulation of the disclosure can comprise one or more buffering agent, for example a pharmaceutically acceptable buffering agent. Buffering agent can be used to control pH of the formulation and/or to maintain stability of the peptidomimetic macrocycle. The pH range of the aqueous pharmaceutical formulation can be pH 2 to pH 12, pH 4 to pH 9, pH 5 to pH9, or pH 6 to pH 8. In some embodiments the aqueous solution is buffered to a pH of about 5.0-9.0. In some embodiments the aqueous pharmaceutical formulation is buffered to a pH of about 6.0-8.0. In some embodiments the pH of the aqueous pharmaceutical formulation is in the range of about 6.5-8.0, about 7.0-8.0, about 7.5-8.0, about 6.0-7.5, about 6.5-7.5, about 7.0-7.5, 6.0-7.0, about 6.5-7.0, about 7.0-7.5, or about 7.5-8.0. In some embodiments the aqueous solution is buffered to a pH of about 6.0, about 6.5, about 7.0, about 7.5, about 8.0 or about 8.5. In some embodiments the aqueous pharmaceutical formulation is buffered to a pH of about 7.0, about 7.1, about 7.2, about 7.3, about 7.4, about 7.5, about 7.6, about 7.7, about 7.8, about 7.9, or about 8.0. In some embodiments the aqueous pharmaceutical formulation is buffered to a pH of about 7.3-7.5.
Any buffering that can be safe for injection into mammalian tissue, particularly into humans, can be used in the pharmaceutical formulation of the disclosure. Buffering agent can be any agent capable of driving an acidic or basic solution to a certain pH state, and then preventing a change from that state. Buffering agents that can be used in the instant aqueous pharmaceutical formulations include citrate, acetate, phosphate, maleate, tartrate, borate, carbonate, bicarbonate, succinate, or glutamate buffers.
In some examples, the buffering agent is lithium lactate, magnesium lactate, sodium lactate, potassium lactate, calcium lactate, lithium phosphate, sodium phosphate, potassium phosphate, calcium phosphate, sodium polyphosphate, potassium polyphosphate, sodium pyrophosphate, potassium pyrophosphate, disodium hydrogenphosphate, dipotassium hydrogenphosphate, trisodium phosphate, tripotassium phosphate, potassium metaphosphate, lithium maleate, sodium maleate, potassium maleate, calcium maleate, lithium tartarate, sodium tartarate, potassium tartarate, calcium tartarate, lithium succinate, sodium succinate, potassium succinate, calcium succinate, lithium acetate, sodium acetate, potassium acetate, calcium acetate, sodium bicarbonate, potassium bicarbonate, magnesium hydroxide, magnesium glucomate, aluminum hydroxide, aluminum hydroxide/sodium bicarbonate coprecipitate, sodium citrate, sodium tartarate, sodium carbonate, magnesium oxide, magnesium hydroxide, magnesium carbonate, magnesium silicate, calcium glycerophosphate, calcium chloride, calcium hydroxide, calcium lactate, calcium carbonate, calcium bicarbonate, or mixture thereof.
In some examples, the buffering agent is a citrate buffer. Non-limiting examples of suitable citrate buffers include lithium citrate monohydrate, sodium citrate monohydrate, potassium citrate monohydrate, calcium citrate monohydrate, lithium citrate dihydrate, sodium citrate dihydrate, potassium citrate dihydrate, calcium citrate dihydrate, lithium citrate trihydrate, sodium citrate trihydrate, potassium citrate trihydrate, calcium citrate trihydrate, lithium citrate tetrahydrate, sodium citrate tetrahydrate, potassium citrate tetrahydrate, calcium citrate tetrahydrate, lithium citrate pentahydrate, sodium citrate pentahydrate, potassium citrate pentahydrate, calcium citrate pentahydrate, lithium citrate hexahydrate, sodium citrate hexahydrate, potassium citrate hexahydrate, calcium citrate hexahydrate, lithium citrate heptahydrate, sodium citrate heptahydrate, potassium citrate heptahydrate, or calcium citrate heptahydrate.
In some examples, the buffering agent is a phosphate buffer. Non-limiting examples of suitable phosphate buffering agents that can be used in the formulations of the instant disclosure include, without limitation, monobasic sodium phosphate, dibasic sodium phosphate, monobasic potassium phosphate, dibasic potassium phosphate, sodium pyrophosphate, potassium pyrophosphate, disodium hydrogenphosphate, dipotassium hydrogenphosphate, potassium metaphosphate, calcium phosphate, tribasic, calcium phosphate, dibasic anhydrous, calcium phosphate dibasic, hydrate, In one embodiment, the buffering agent is a phosphate buffer. In one embodiment buffering agent is NaH₂PO₄. In one embodiment, the buffering agent is Na₂HPO₄. In one embodiment the buffering agent is a mixture of NaH₂PO₄and Na₂HPO₄. In one embodiment buffering agent is KH₂PO₄. In one embodiment, the buffering agent is K₂HPO₄. In one embodiment the buffering agent is a mixture of KH₂PO₄and K₂HPO₄.

Tonicity Adjusting Agents

The aqueous pharmaceutical formulations disclosed herein can comprise one or more tonicity adjusting agents in order to adjust the tonicity/osmolarity of the formulations. For example, the tonicity/osmolarity of the aqueous pharmaceutical formulations can be adjusted to be isotonic with human plasma. This can help to avoid damage to the tissues. In various embodiments, the osmolarity of the aqueous pharmaceutical formulations disclosed herein can be in the range of 250 to 1000 mOsM. For example, the osmolarity of the formulations can be about 250-300 mOsM, 250-350 mOsM, 250-400 mOsM, 250-450 mOsM, 250-500 mOsM, 250-550 mOsM, 250-600 mOsM, 250-650 mOsM, 250-700 mOsM, 250-750 mOsM, 250-800 mOsM, 250-850 mOsM, 250-900 mOsM, 250-950 mOsM, 300-350 mOsM, 300-400 mOsM, 300-450 mOsM, 300-500 mOsM, 300-550 mOsM, 300-600 mOsM, 300-650 mOsM, 300-700 mOsM, 300-750 mOsM, 300-800 mOsM, 300-850 mOsM, 300-900 mOsM, 300-950 mOsM, 300-1000 mOsM, 350-400 mOsM, 350-450 mOsM, 350-500 mOsM, 350-550 mOsM, 350-600 mOsM, 350-650 mOsM, 350-700 mOsM, 350-750 mOsM, 350-800 mOsM, 350-850 mOsM, 350-900 mOsM, 350-950 mOsM, 350-1000 mOsM, 400-450 mOsM, 400-500 mOsM, 400-550 mOsM, 400-600 mOsM, 400-650 mOsM, 400-700 mOsM, 400-750 mOsM, 400-800 mOsM, 400-850 mOsM, 400-900 mOsM, 400-950 mOsM, 400-1000 mOsM, 450-500 mOsM, 450-550 mOsM, 450-600 mOsM, 450-650 mOsM, 450-700 mOsM, 450-750 mOsM, 450-800 mOsM, 450-850 mOsM, 450-900 mOsM, 450-950 mOsM, 450-1000 mOsM, 500-550 mOsM, 500-600 mOsM, 500-650 mOsM, 500-700 mOsM, 500-750 mOsM, 500-800 mOsM, 500-850 mOsM, 500-900 mOsM, 500-950 mOsM, 500-1000 mOsM, 550-600 mOsM, 550-650 mOsM, 550-700 mOsM, 550-750 mOsM, 550-800 mOsM, 550-850 mOsM, 550-900 mOsM, 550-950 mOsM, 550-1000 mOsM, 600-650 mOsM, 600-700 mOsM, 600-750 mOsM, 600-800 mOsM, 600-850 mOsM, 600-900 mOsM, 600-950 mOsM, 600-1000 mOsM, 650-700 mOsM, 650-750 mOsM, 650-800 mOsM, 650-850 mOsM, 650-900 mOsM, 650-950 mOsM, 650-1000 mOsM, 700-750 mOsM, 700-800 mOsM, 700-850 mOsM, 700-900 mOsM, 700-950 mOsM, 700-1000 mOsM, 750-800 mOsM, 750-850 mOsM, 750-900 mOsM, 750-950 mOsM, 750-1000 mOsM, 800-850 mOsM, 800-900 mOsM, 800-950 mOsM, 800-1000 mOsM, 850-900 mOsM, 850-950 mOsM, 850-1000 mOsM, 900-950 mOsM, 900-1000 mOsM, or 950-1000 mOsM. In some embodiments, the osmolarity of the formulations is in the range of 250 to 450 mOsM. For example the osmolarity of the formulations can be about 250 mOsM, about 300 mOsM, about 350 mOsM, about 400 mOsM, or about 450 mOsM. In some embodiments, the formulation is isotonic with biologic fluids, i.e., the osmolarity is about 300 mOsM.
The tonicity adjusting agents can be ionic tonicity adjusting agents or non-ionic tonicity adjusting agents. In some embodiments, the isotonic agent is an ionic-isotonic agent. In some embodiments, the isotonic agent is a non-ionic isotonic agent. In some embodiments, the isotonic agent is a mixture of one or more ionic and/or non-ionic isotonic agent. In a some embodiment of the disclosure the isotonic agent is selected from the group consisting of a salt (e.g. sodium chloride, boric acid, sodium nitrate, potassium nitrate), a sugar or sugar alcohol, an amino acid (e.g. L-glycine, L-histidine, arginine, lysine, isoleucine, aspartic acid, tryptophan, threonine), an alditol (e.g. glycerol (glycerine), 1,2-propanediol (propyleneglycol), 1,3-propanediol, 1,3-butanediol, polyethyleneglycol (e.g. PEG400), or mixtures thereof. Any sugar such as mono-, di-, or polysaccharides, or water-soluble glucans, including for example fructose, glucose, mannose, sorbose, xylose, maltose, lactose, sucrose, trehalose, dextran, pullulan, dextrin, cyclodextrin, soluble starch, hydroxyethyl starch and carboxymethylcellulose-Na can be used. In some examples, the tonicity adjusting agent is selected from a group consisting of dextrose, glycerin, mannitol, trehalose, potassium chloride and sodium chloride. In some example, the tonicity adjusting agent is trehalose, for example D-trehalose. In some example, the tonicity adjusting agent is sodium chloride. In some example, the tonicity adjusting agent is potassium chloride. The use of an tonicity adjusting agent in aqueous pharmaceutical formulations is well-known to the skilled person. For convenience reference is made to Remington: The Science and Practice of Pharmacy, 19^thedition, 1995.

Stabilizing Agent

The aqueous pharmaceutical formulations described herein comprise a stabilizing agent. Non-limiting examples of stabilizing agents that can be used include acacia, agar, albumin, alginic acid, aluminum stearate, ammonium alginate, arabinose, arginine HCL, ascorbic acid, ascorbyl palmitate, bentonite, butylated hydroxytoluene, calcium alginate, calcium stearate, carboxymethylcellulose calcium, carboxymethylcellulose sodium, carrageenan, cellobiose, cellulose ceratonia, colloidal silicon dioxide, cyclodextrins, diethanolamine, dextran, edentates, ethylcellulose, ethylene glycol palmitostearate, fructose, gentiobiose, glucose, glucosamine, glycine, glycerin monostearate, hydroxypropyl cellulose, hydroxyethyl starch, hypromellose, hyaluronic acid, invert sugar, isomaltose, lactose, lecithin, magnesium aluminum silicate, mannose, mannitol, maltose, mineral oil and lanolin alcohols, monoethanolamine, N-methyl pyrollidone, pectin, polacrilin potassium, poloxamer (for example poloxamer 124, poloxamer 188, poloxamer 237, poloxamer 338, or poloxamer 407), polyoxyethylene sobitan fatty acid esters, polyvinyl alcohol, potassium alginate, potassium chloride, povidone (for example povidone K-12, povidone K-15, povidone K-17, povidone K-25, povidone K-20, povidone K-60, povidone K-90, or povidone K-120), propyl gallate, propylene glycol, propylene glycol alginate, raffinose, sodium acetate, sodium alginate, sodium borate, sodium chloride, sodium stearyl fumarate, sorbitol, stearyl alcohol, sucrose, sulfobutylether β-cyclodextrin, starch, trehalose, white wax, xanthan gum, xylitol, yellow wax and zinc acetate.
In some embodiments, the stabilizing agent is a polyoxyethylene sobitan fatty acid ester, for example polysorbate 20, polysorbate 21, polysorbate 40, polysorbate 60, polysorbate 61, polysorbate 65, polysorbate 80, polysorbate 81, polysorbate 85 or polysorbate 120. In some embodiments, the stabilizing agent is polysorbate 20. In some embodiments, the stabilizing agent is polysorbate 21. In some embodiments, the stabilizing agent is polysorbate 40. In some embodiments, the stabilizing agent is polysorbate 60. In some embodiments, the stabilizing agent is polysorbate 61. In some embodiments, the stabilizing agent is polysorbate 65. In some embodiments, the stabilizing agent is polysorbate 80. In some embodiments, the stabilizing agent is polysorbate 81. In some embodiments, the stabilizing agent is polysorbate 85. In some embodiments, the stabilizing agent is polysorbate 120.

Preservatives-Antioxidants, Antimicrobial and Chelating Agents

The aqueous pharmaceutical formulations disclosed herein can comprise one or more antioxidants in order to prevent/minimize the oxidation of the peptidomimetic macrocycle and/or the excipients present in the formulation The antioxidants can also be used as a stabilizing agent. The anti-oxidants which can be used to form aqueous pharmaceutical formulations the disclosure include, but are not limited to, propyl, octyl and dodecyl esters of gallic acid, butylated hydroxyanisole (BHA, usually purchased as a mixture of ortho and meta isomers), green tea extract, uric acid, cysteine, pyruvate, nordihydroguaiaretic acid, ascorbic acid, salts of ascorbic acid such as ascorbyl palmitate and sodium ascorbate, ascorbyl glucosamine, vitamin E (i.e., tocopherols such as α-tocopherol), derivatives of vitamin E (e.g., tocopheryl acetate), retinoids such as retinoic acid, retinol, trans-retinol, cis-retinol, mixtures of trans-retinol and cis-retinol, 3-dehydroretinol and derivatives of vitamin A (e.g., retinyl acetate, retinal and retinyl palmitate, also known as tetinyl palmitate), sodium citrate, sodium sulfite, sodium thiosulfate, sodium bisulfate, lycopene, anthocyanids, bioflavinoids (e.g., hesperitin, naringen, rutin and quercetin), superoxide dismutase, glutathione peroxidase, butylated hydroxytoluene (BHT), indole-3-carbinol, pycnogenol, melatonin, sulforaphane, pregnenolone, lipoic acid and 4-hydroxy-5-methyl-3[2H]-furanone. In various embodiments, one or more of the above antioxidants are excluded, or are present in less than effective amounts.
In some embodiments the antioxidant is ascorbic acid, citric acid, acetylcysteine, sulfurous acid salts (such as bisulfite, metasulfite), and monothioglyercol.
The aqueous pharmaceutical formulations can comprise one or more antimicrobial agent. Suitable antimicrobial agents that can be used include alcohol, benzalkonium chloride, benzyl alcohol, boric acid, bronopol, butylated hydroxyanisole, butylparaben, carbon dioxide, bentonite, cetrimide, cetylpyridinium chloride, chlorbutanol, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol (meta cresol), dimethyl ether, ethylparaben, glycerin, hexetidine, imidurea, inactivation by magnesium trisilicate, isopropyl alcohol, lactic acid, methylparaben, monothioglycerol, parabens (methyl, propyl, butyl), phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric salts (acetate, borate, nitrate) phenylmercuric borate, phenylmercuric nitrate, potassium benzoate, potassium metabisulfite, potassium sorbate, propionic acid, propyl gallate, propylene glycol, propylparaben, sodium acetate, sodium benzoate, sodium borate, sodium lactate, sodium metabisulfite, sodium propionate, sodium sulfite, sorbic acid, synergists, edetic acid, thimerosal, xylitol, or other agents known to those skilled in the art. In some embodiments, the antimicrobial agent used is methyl paraben, ethyl paraben, propyl paraben, or a combination thereof. In some embodiments, the antimicrobial agent used is benzalkonium chloride.
The aqueous pharmaceutical formulations disclosed herein can comprise one or more chelating agents. Non-limiting examples of chelating agents which can be used to form aqueous pharmaceutical formulations of the disclosure include, but are not limited to, ethylene diaminetetraacetic acid (EDTA), EDTA disodium, calcium disodium edetate, EDTA trisodium, albumin, transferrin, desferoxamine, desferal, desferoxamine mesylate, EDTA tetrasodium and EDTA dipotassium, sodium metasilicate, citric acid monohydrate, fumaric acid, malic acid, maltol, or combinations of any of these. In some embodiments, the formulations of the current disclosure contain no or essentially no chelating agents. In some further embodiments, the formulations are solutions containing no chelating agents.
In some embodiments, the aqueous pharmaceutical formulations of the disclosure comprise no or essentially no preservatives. In some further embodiments, the aqueous pharmaceutical formulations are solutions containing no preservatives.

Surfactants

The solubility of the components of the present formulations can be enhanced by a surfactant or other appropriate co-solvent in the composition. Such co-solvents include polysorbate 20, 60, and 80, Pluronic® F68, F-84 and P-103, cyclodextrin, or other agents known to those skilled in the art. Such co-solvents can be employed at a level of from about 0.010% to 2% by weight. In addition, the surfactant can be used to prevent aggregation of the compound.
Surfactants which can be used to form aqueous pharmaceutical formulations include, but are not limited to, hydrophilic surfactants, lipophilic surfactants, and mixtures thereof. That is, a mixture of hydrophilic surfactants can be employed, a mixture of lipophilic surfactants can be employed, or a mixture of at least one hydrophilic surfactant and at least one lipophilic surfactant can be employed.
In some embodiments of the disclosure, the surfactant can be the sodium salt form of the compound, which can include the monosodium salt form. Suitable sodium salt surfactants can be selected based on desirable properties, including high speed of polymerization, small resultant particle sizes suitable for delivery, good polymerization yields, stability including freeze-thaw and shelf-life stability, improved surface tension properties, and lubrication properties.
The surfactant can be any suitable, non-toxic compound that is non-reactive with the medicament and that substantially reduces the surface tension between the medicament, the excipient and the site of administration. Some useful surfactants are: oleic acid available under the trade names Mednique 6322 and Emersol 6321 (from Cognis Corp., Cincinnati, Ohio); cetylpyridinium chloride (from Arrow Chemical, Inc. Westwood, N.J.); soya lecithin available under the trade name Epikuron 200 (from Lucas Meyer Decatur, Ill.); polyoxyethylene(20) sorbitan monolaurate available under the tradename Tween 20 (from ICI Specialty Chemicals, Wilmington, Del.); polyoxyethylene(20) sorbitan monostearate available under the tradename Tween 60 (from ICI); polyoxyethylene(20) sorbitan monooleate available under the tradename Tween 80 (from ICI); polyoxyethylene (10) stearyl ether available under the tradename Brij 76 (from ICI); polyoxyethylene (2) oleyl ether available under the tradename Brij 92 (frown ICI); Polyoxyethylene-polyoxypropylene-ethylenediamine block copolymer available under the tradename Tetronic 150 R1 (from BASF); polyoxypropylene-polyoxyethylene block copolymers available under the trade names Pluronic L-92, Pluronic L-121 end Pluronic F 68 (from BASF); castor oil ethoxylate available under the tradename Alkasurf CO-40 (from Rhone-Poulenc Mississauga Ontario, Canada); and mixtures thereof.
A suitable hydrophilic surfactant can generally have an HLB value of at least 10, while suitable lipophilic surfactants can generally have an HLB value of or less than about 10. An empirical parameter used to characterize the relative hydrophilicity and hydrophobicity of non-ionic amphiphilic compounds is the hydrophilic-lipophilic balance (“HLB” value). Surfactants with lower HLB values are more lipophilic or hydrophobic, and have greater solubility in oils, while surfactants with higher HLB values are more hydrophilic, and have greater solubility in aqueous solutions. Hydrophilic surfactants are generally considered to be those compounds having an HLB value greater than about 10, as well as anionic, cationic, or zwitterionic compounds for which the HLB scale is not generally applicable. Similarly, lipophilic (i.e., hydrophobic) surfactants are compounds having an HLB value equal to or less than about 10. However, HLB value of a surfactant is merely a rough guide generally used to enable formulation of industrial, pharmaceutical and cosmetic emulsions.
Hydrophilic surfactants can be either ionic or non-ionic. Suitable ionic surfactants include, but are not limited to, alkylammonium salts; fusidic acid salts; fatty acid derivatives of amino acids, oligopeptides, and polypeptides; glyceride derivatives of amino acids, oligopeptides, and polypeptides; lecithins and hydrogenated lecithins; lysolecithins and hydrogenated lysolecithins; phospholipids and derivatives thereof, lysophospholipids and derivatives thereof, camitine fatty acid ester salts; salts of alkylsulfates; fatty acid salts; sodium docusate; acyl lactylates; mono- and di-acetylated tartaric acid esters of mono- and di-glycerides; succinylated mono- and di-glycerides; citric acid esters of mono- and di-glycerides; and mixtures thereof.
Within the aforementioned group, some ionic surfactants include, by way of example: lecithins, lysolecithin, phospholipids, lysophospholipids and derivatives thereof, carnitine fatty acid ester salts; salts of alkylsulfates; fatty acid salts; sodium docusate; acyl lactylates; mono- and di-acetylated tartaric acid esters of mono- and di-glycerides; succinylated mono- and di-glycerides; citric acid esters of mono- and di-glycerides; and mixtures thereof.
Ionic surfactants can be the ionized forms of lecithin, lysolecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, phosphatidylserine, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidic acid, lysophosphatidylserine, PEG-phosphatidylethanolamine, PVP-phosphatidylethanolamine, lactylic esters of fatty acids, stearoyl-2-lactylate, stearoyl lactylate, succinylated monoglycerides, mono/diacetylated tartaric acid esters of mono/diglycerides, citric acid esters of mono/diglycerides, cholylsarcosine, caproate, caprylate, caprate, laurate, myristate, palmitate, oleate, ricinoleate, linoleate, linolenate, stearate, lauryl sulfate, teracecyl sulfate, docusate, lauroyl carnitines, palmitoyl carnitines, myristoyl carnitines, and salts and mixtures thereof.
Hydrophilic non-ionic surfactants can include, but not limited to, alkylglucosides; alkylmaltosides; alkylthioglucosides; lauryl macrogolglycerides; polyoxyalkylene alkyl ethers such as polyethylene glycol alkyl ethers; polyoxyalkylene alkylphenols such as polyethylene glycol alkyl phenols; polyoxyalkylene alkyl phenol fatty acid esters such as polyethylene glycol fatty acids monoesters and polyethylene glycol fatty acids diesters; polyethylene glycol glycerol fatty acid esters; polyglycerol fatty acid esters; polyoxyalkylene sorbitan fatty acid esters such as polyethylene glycol sorbitan fatty acid esters; hydrophilic transesterification products of a polyol with at least one member of the group consisting of glycerides, vegetable oils, hydrogenated vegetable oils, fatty acids, and sterols; polyoxyethylene sterols, derivatives, and analogues thereof, polyoxyethylated vitamins and derivatives thereof, polyoxyethylene-polyoxypropylene block copolymers; and mixtures thereof, polyethylene glycol sorbitan fatty acid esters and hydrophilic transesterification products of a polyol with at least one member of the group consisting of triglycerides, vegetable oils, and hydrogenated vegetable oils. The polyol can be glycerol, ethylene glycol, polyethylene glycol, sorbitol, propylene glycol, pentaerythritol, or a saccharide.
Other hydrophilic-non-ionic surfactants include, without limitation, PEG-10 laurate, PEG-12 laurate, PEG-20 laurate, PEG-32 laurate, PEG-32 dilaurate, PEG-12 oleate, PEG-15 oleate, PEG-20 oleate, PEG-20 dioleate, PEG-32 oleate, PEG-200 oleate, PEG-400 oleate, PEG-15 stearate, PEG-32 distearate, PEG-40 stearate, PEG-100 stearate, PEG-20 dilaurate, PEG-25 glyceryl trioleate, PEG-32 dioleate, PEG-20 glyceryl laurate, PEG-30 glyceryl laurate, PEG-20 glyceryl stearate, PEG-20 glyceryl oleate, PEG-30 glyceryl oleate, PEG-30 glyceryl laurate, PEG-40 glyceryl laurate, PEG-40 palm kernel oil, PEG-50 hydrogenated castor oil, PEG-40 castor oil, PEG-35 castor oil, PEG-60 castor oil, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-60 corn oil, PEG-6 caprate/caprylate glycerides, PEG-8 caprate/caprylate glycerides, polyglyceryl-10 laurate, PEG-30 cholesterol, PEG-25 phyto sterol, PEG-30 soya sterol, PEG-20 trioleate, PEG-40 sorbitan oleate, PEG-80 sorbitan laurate, polysorbate 20, polysorbate 80, POE-9 lauryl ether, POE-23 lauryl ether, POE-10 oleyl ether, POE-20 oleyl ether, POE-20 stearyl ether, tocopheryl PEG-100 succinate, PEG-24 cholesterol, polyglyceryl-10oleate, Tween 40, Tween 60, sucrose monostearate, sucrose monolaurate, sucrose monopalmitate, PEG 10-100 nonyl phenol series, PEG 15-100 octyl phenol series, and poloxamers.
Suitable lipophilic surfactants include, by way of example only: fatty alcohols; glycerol fatty acid esters; acetylated glycerol fatty acid esters; lower alcohol fatty acids esters; propylene glycol fatty acid esters; sorbitan fatty acid esters; polyethylene glycol sorbitan fatty acid esters; sterols and sterol derivatives; polyoxyethylated sterols and sterol derivatives; polyethylene glycol alkyl ethers; sugar esters; sugar ethers; lactic acid derivatives of mono- and di-glycerides; hydrophobic transesterification products of a polyol with at least one member of the group consisting of glycerides, vegetable oils, hydrogenated vegetable oils, fatty acids and sterols; oil-soluble vitamins/vitamin derivatives; and mixtures thereof. Within this group, some lipophilic surfactants include glycerol fatty acid esters, propylene glycol fatty acid esters, and mixtures thereof, or are hydrophobic transesterification products of a polyol with at least one member of the group consisting of vegetable oils, hydrogenated vegetable oils, and triglycerides.
In some embodiments the formulations of the disclosure contain no surfactants. In some embodiments, the formulations of the disclosure are intravenous formulations containing no surfactants. In some further embodiments the formulations contain substantially no surfactant, i.e. contain less than approximately 0.0001% by weight of surfactants. In some embodiments, the formulations contain essentially no surfactants.
If desired, however, the formulations can contain surface-active agents conventionally employed, such as oleic acid, lecithin, sorbitan trioleate, cetylpyridinium chloride, benzalkonium chloride, polyoxyethylene (20) sorbitan monolaurate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene (20) sorbitan mono-oleate, polyoxypropylene/polyoxyethylene block copolymers, polyoxypropylene/polyoxyethylene/ethylene diamine block copolymers, ethoxylated castor oil and the like, where the proportion of surface-active agents, if present, can be about 0.0001 to 10% by weight, or about 0.001 to 0.10% by weight, based on the total formulation. Other suitable surfactant/emulsifying agents would be known to one of skill in the art and are listed in the CTFA International Cosmetic Ingredient Dictionary and Handbook, Vol. 2, 7th Edition (1997).
The aqueous pharmaceutical formulations of the disclosure can further include other pharmacological active ingredients as far as they do not contradict the purpose of the present disclosure. The aqueous pharmaceutical formulations for example can comprise solubilizing agents, bulking agents, dissolution enhancers, wetting agents, emulsifiers, suspending agents, antibacterial agents, sweeteners, perfuming agents, flavoring agents, and combinations thereof.
Some of the excipients or additives can have more than one possible function or use, depending on their properties and the nature of the formulation. In a combination of plural active ingredients, their respective contents can be suitably increased or decreased in consideration of their effects and safety.

Peptidomimetic Macrocycles

In some embodiments, a peptidomimetic macrocycle has the Formula (I):
wherein:
each A, C, and D is independently an amino acid;
each B is independently an amino acid,
[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];
each E is independently an amino acid selected from the group consisting of Ala (alanine), D-Ala (D-alanine), Aib (α-aminoisobutyric acid), Sar (N-methyl glycine), and Ser (serine);
each R₃independently is hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
each R₁and R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-; or forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids;
each L and L′ is independently a macrocycle-forming linker;
each L₃is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
each v is independently an integer;
each w is independently an integer from 3-1000;
u is an integer from 1-10;
each x, y and z is independently an integer from 0-10; and
each n is independently an integer from 1-5.
In some embodiments, each v and w is independently integers between 1-30. In some embodiments, w is an integer from 3-1000, for example 3-500, 3-200, 3-100, 3-50, 3-30, 3-20, or 3-10. In some embodiments, the sum of x+y+z is 3 or 6. In some embodiments, the sum of x+y+z is 3. In other embodiments, the sum of x+y+z is 6.
In some embodiments, peptidomimetic macrocycles are also provided of the formula:
wherein:
each of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀is individually an amino acid, wherein at least three of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3), where each X is an amino acid;
each D and E is independently an amino acid;
each R₁and R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-; or at least one of R₁and R₂forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids; each L or L′ is independently a macrocycle-forming linker;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
v is an integer from 1-1000, for example 1-500, 1-200, 1-100, 1-50, 1-30, 1-20 or 1-10; and
w is an integer from 3-1000, for example 3-500, 3-200, 3-100, 3-50, 3-30, 3-20, or 3-10.
In some embodiments, each v and w is independently an integer between 1-30. In some embodiments, w is an integer from 3-1000, for example 3-500, 3-200, 3-100, 3-50, 3-30, 3-20, or 3-10. In some embodiments, the sum of x+y+z is 3 or 6. In some embodiments, the sum of x+y+z is 3. In other embodiments, the sum of x+y+z is 6.
In some embodiments of any of the Formulas described herein, at least three of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3). In other embodiments, at least four of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3). In other embodiments, at least five of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3). In other embodiments, at least six of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3). In other embodiments, at least seven of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-His₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀-X₁₁-Ser₁₂(SEQ ID NO: 3).
In some embodiments, a peptidomimetic macrocycle has the Formula:
wherein:

- each of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀is individually an amino acid, wherein at least three of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4), where each X is an amino acid;
- each D is independently an amino acid;
- each E is independently an amino acid, for example an amino acid selected from Ala (alanine), D-Ala (D-alanine), Aib (α-aminoisobutyric acid), Sar (N-methyl glycine), and Ser (serine);

each R₁and R₂are independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-; or at least one of R₁and R₂forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids;
each L or L′ is independently a macrocycle-forming linker;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
v is an integer from 1-1000, for example 1-500, 1-200, 1-100, 1-50, 1-30, 1-20, or 1-10; w is an integer from 3-1000, for example 3-500, 3-200, 3-100, 3-50, 3-30, 3-20, or 3-10; and.
In some embodiments of the above Formula, at least three of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4). In other embodiments of the above Formula, at least four of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4). In other embodiments of the above Formula, at least five of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4) In other embodiments of the above Formula, at least six of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4). In other embodiments of the above Formula, at least seven of Xaa₃, Xaa₅, Xaa₆, Xaa₇, Xaa₈, Xaa₉, and Xaa₁₀are the same amino acid as the amino acid at the corresponding position of the sequence Phe₃-X₄-Glu₅-Tyr₆-Trp₇-Ala₈-Gln₉-Leu₁₀/Cba₁₀-X₁₁-Ala₁₂(SEQ ID NO: 4).
In some embodiments, w is an integer from 3-10, for example 3-6, 3-8, 6-8, or 6-10. In some embodiments, w is 3. In other embodiments, w is 6. In some embodiments, v is an integer from 1-10, for example 2-5. In some embodiments, v is 2.
In one embodiment, the peptidomimetic macrocycle of Formula (I) is Formula (Ia):
or a pharmaceutically-acceptable salt thereof wherein:
each of Xaa₆, Xaa₇, Xaa₈, Xaa₁₀, Xaa₁₁, Xaa₁₂, and Xaa₁₃is independently an amino acid, wherein at least three, four, five, or each of Xaa₆, Xaa₇, Xaa₈, Xaa₁₀, Xaa₁₁, Xaa₁₂, are the same amino acid as the amino acid at the corresponding position of the sequence X₅-Thr₆-Leu₇-Leu₈-X₉-Leu₁₀-Lys₁₁/Ala₁₁-Val₁₂/Ala₁₂(SEQ ID NO: 6), where each of X₅and X₉is independently an amino acid.
In some embodiments, the peptidomimetic macrocycle of Formula (Ia) is Formula (Ia-1):
or a pharmaceutically-acceptable salt thereof, wherein each Xaa₁₄is independently an amino acid.
In some embodiments, the peptidomimetic macrocycle of Formula (Ia) is Formula (Ia-2):
or a pharmaceutically-acceptable salt thereof, wherein each Xaa₁₄and Xaa₁₅is independently an amino acid.
In one embodiment, the peptidomimetic macrocycle of Formula (I) is Formula (Ib):
or a pharmaceutically-acceptable salt thereof, wherein: each of Xaa₆, Xaa₇, Xaa₈, Xaa₉, Xaa₁₀, Xaa₁₁and Xaa₁₃is independently an amino acid, wherein at least three, four, five, or each of Xaa₆, Xaa₇, Xaa₈, Xaa₉, Xaa₁₀, and Xaa₁₁are the same amino acid as the amino acid at the corresponding position of the sequence X₅-Thr₆-Leu₇-Leu₈-Phe₉-Leu₁₀-Lys₁₁/Ala₁₁-X₁₂(SEQ ID NO: 7), where each of X₅and X₁₂is independently an amino acid.
In some embodiments, the peptidomimetic macrocycle of Formula (Ib) is Formula (Ib-1):
or a pharmaceutically-acceptable salt thereof, wherein each Xaa₁₄is independently an amino acid.
In some embodiments, the peptidomimetic macrocycle of Formula (Ib) is
or a pharmaceutically-acceptable salt thereof, wherein each Xaa₁₄and Xaa₁₅is independently an amino acid.
In some embodiments, the invention provides a peptidomimetic macrocycle of Formula (IX):
wherein the peptidomimetic macrocycle binds MCL-1 selectively over another protein that has a BH3 domain, wherein:
each A, C, D, and E is independently a natural or non-natural amino acid;
each B is independently a natural or non-natural amino acid, amino acid analog,
[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];
each L is independently a macrocycle-forming linker;
each L′ is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene, each being optionally substituted with R₅, or a bond, or together with R₁and the atom to which both R₁and L′ are bound forms a ring;
each L″ is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene, each being optionally substituted with R₅, or a bond, or together with R₂and the atom to which both R₂and L″ are bound forms a ring;
each R₁is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-, or together with L′ and the atom to which both R₁and L′ are bound forms a ring;
each R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-, or together with L″ and the atom to which both R₂and L″ are bound forms a ring;
each R₃is independently hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, aryl, or heteroaryl, optionally substituted with R₅;
each L₃is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, heteroarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each n is independently an integer from 1-5;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope, or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope, or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue; each v and w is independently an integer from 1-1000;
u is an integer from 1-10; and
each x, y and z is independently an integer from 0-10, or
a pharmaceutically-acceptable salt thereof.
In some embodiments, the invention provides a peptidomimetic macrocycle having the formula (SEQ ID NO: 8):
wherein:
each D and E is independently an amino acid residue;
each R¹and R²are independently alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, each being optionally substituted with halo-; —H, or at least one of R¹and R²forms a macrocycle-forming linker L′ connected to the alpha position of one of the D or E amino acid residues;
each L is a macrocycle-forming linker of the formula -L′-L²- or -L^t-L²-L³-;
each L¹, L², and L³are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, heteroarylene, or [—R⁴—K—R⁴—]_n, each being optionally substituted with R⁵;
each R³is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, each being optionally substituted with R⁵;
each R⁴is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene, each being optionally substituted with R⁵;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR³;
each R⁵is independently halogen, alkyl, —OR, —N(R⁶)₂, —SR⁶, —SOR⁶, —SO₂R⁶, —CO₂R⁶, a fluorescent moiety, a radioisotope, or a therapeutic agent;
each R⁶is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope, or a therapeutic agent;
R⁷is —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, each being optionally substituted with R⁵, or part of a cyclic structure with a D residue;
R⁸is —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, each being optionally substituted with R⁵, or part of a cyclic structure with an E residue;
each of Xaa¹and Xaa²is independently an amino acid residue or absent;
Xaa³is Ala, Aib, Asp, Asn, Cys, Glu, Gln, His, Ile, Lys, Leu, Met, Arg, Ser, Thr, Val, Trp, Tyr, or an analog of any of the foregoing;
v is an integer from 1-1000;
w is an integer from 0-1000; and
n is an integer from 1-5, or
a pharmaceutically-acceptable salt thereof.
In some embodiments, the invention provides a peptidomimetic macrocycle of the formula:
wherein:
each D and E is independently an amino acid residue;
R¹and R²are independently alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, each being optionally substituted with halo-; —H, or at least one of R¹and R²forms a macrocycle-forming linker L′ connected to the alpha position of one of the D or E amino acid residues;
each L or L′ is independently a macrocycle-forming linker of the formula -L¹-L²- or -L¹-L²-L³-L^t, L², and L³are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, heteroarylene, or [—R⁴—K—R⁴—]_n, each being optionally substituted with R⁵; each R³is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, each being optionally substituted with R⁵;
each R⁴is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene, each being optionally substituted with R⁵;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR³;
each R⁵is independently halogen, alkyl, —OR⁶, —N(R⁶)₂, —SR⁶, —SOR⁶, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope, or a therapeutic agent;
each R⁶is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope, or a therapeutic agent;
R⁷is —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, each being optionally substituted with R⁵, or part of a cyclic structure with a D residue;
R⁸is —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, each being optionally substituted with R⁵, or part of a cyclic structure with an E residue;
each of Xaa¹and Xaa²is independently an amino acid residue or absent;
v is an integer from 1-1000;
w is an integer from 0-1000; and
n is an integer from 1-5, or
a pharmaceutically-acceptable salt thereof.
In some embodiments, the invention provides a peptidomimetic macrocycle comprising an amino acid sequence of formula:
X₁-X₂-X₃-X₄-X₅-X₆-X₇-X₈-X₉-X₁₀-X₁₁-X₁₂-X₁₃-X₁₄-X₁₅-X₁₆-X₁₇-X₁₈-X₁₉-X₂₀-X₂₁
wherein:
X₁is Ile, Arg, Ala, Lys, Pro, Leu, Asp, Glu, His, Ser, Gln, Phe, an analog thereof, or absent;
X₂is Trp, Arg, Ala, Asn, Phe, Pro, Leu, Ser, Lys, Tyr, His, Cou, Cou2, Cou4, Cou7, an analog thereof, a crosslinked amino acid, or absent;
X₃is Ile, Ala, Leu, Phe, Tyr, Val, Asp, Trp, Pro, Gln, Chg, Ac5c, Ac6c, Tba, Bip, Cha, Adm, hCha, an analog thereof, or absent;
X₄is Ala, Gln, Asp, Val, Gly, Ser, Leu, Phe, Cha, A4, an analog, thereof, a crosslinked amino acid, or absent;
X₅is Gln, Ala, Leu, Phe, Tyr, Gly, Ile, Val, Arg, Glu, Pro, Asp, MO, MO2, an analog thereof, a crosslinked amino acid, or absent;
X₆is Glu, Gln, His, Ala, Ser, Arg, Ile, Leu, Thr, Phe, Val, Tyr, Gly, Nle, St, an analog thereof, or absent;
X₇is Ala, Leu, Phe, Ile, 2Nal, 1Nal, 3cf, Chg, Cha, Adm, hCha, Igl, Bip, an analog thereof, or absent;
X₈is Arg, Ala, Asp, Glu, Thr, His, Gln, Gly, Asn, Phe, Cit, St, an analog thereof, a crosslinked amino acid, or absent;
X₉is Arg, Ala, Asp, Lys, Asn, Gly, Ser, Gln, Cys, Nle, St, an analog thereof, or a crosslinked amino acid;
X₁₀is Ile, Val, Ala, Asp, Asn, Phe, Tba, hL, hhL, Nle, Chg, Cha, an analog thereof, or a crosslinked amino acid;
X₁₁is Gly, Val, Ala, Leu, Ile, Asp, Glu, Cha, Aib, Abu, an analog thereof, or a crosslinked amino acid;
X₁₂is Asp, Ala, Asn, Gly, Arg, Glu, Lys, Leu, Nle, an analog thereof, or a crosslinked amino acid;
X₁₃is Ala, Glu, Gln, Leu, Lys, Asp, Tyr, Ile, Ser, Cys, St, Sta5, Aib, Nle, an analog thereof, or a crosslinked amino acid;
X₁₄is Phe, Ala, Leu, Val, Tyr, Glu, His, Ile, Nle, 1Nal, 2Nal, Chg, Cha, BiP, an analog thereof, or a crosslinked amino acid;
X₁₅is Asn, Gln, Ser, His, Glu, Asp, Ala, Leu, Ile, St, Nle, Aib, an analog thereof, a crosslinked amino acid, or absent;
X₁₆is Ala, Glu, Asp, Arg, Lys, Phe, Gly, Gln, Aib, Cha, St, an analog thereof, a crosslinked amino acid, or absent;
X₁₇is Phe, Tyr, Ala, Leu, Asn, Ser, Gln, Arg, His, Thr, Cou2, Cou3, Cou7, Dpr, Amf, Damf, Amye, an analog thereof, a crosslinked amino acid, or absent;
X₁₈is Tyr, Ala, Ile, Phe, His, Arg, Lys, Trp, Orn, Amf, Amye, Cha, 2Nal, an analog thereof, or absent;
X₁₉is Ala, Lys, Arg, His, Ser, Gln, Glu, Asp, Thr, Aib, Cha, an analog thereof, a crosslinked amino acid, or absent; and
X₂₀is Arg, His, Ala, Thr, Lys, Amr, an analog thereof, a crosslinked amino acid, or absent; and
X₂₁is Arg, His, Ala, Amr, an analog thereof, or absent, or
a pharmaceutically-acceptable salt thereof,
wherein at least two of the amino acids of the amino acid sequence are a crosslinked amino acid.
In some embodiments, the invention provides a peptidomimetic macrocycle comprising an amino acid sequence with C-terminal amino acid residues that are -His-His, wherein the peptidomimetic macrocycle comprises a crosslink connecting at least two amino acid residues, or a pharmaceutically-acceptable salt thereof. In an embodiment of any of the Formulas described herein, of the macrocycle-forming linker (L or L′) has a formula -L₁-L₂-, wherein
L₁and L₂are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each R₃independently is hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅; and
n is an integer from 1-5.
In some embodiments, L (or L′) is a macrocycle-forming linker of the formula
Exemplary embodiments of such macrocycle-forming linkers L are shown below.
In an embodiment of any of the Formulas described herein, L₁and L₂, either alone or in combination, form a triazole or a thioether.
In an embodiment of any of the Formulas described herein, L₁and L₂, either alone or in combination, do not form a triazole or a thioether.
In one example, at least one of R₁and R₂is alkyl, unsubstituted or substituted with halo-. In another example, both R₁and R₂are independently alkyl, unsubstituted or substituted with halo-. In some embodiments, at least one of R₁and R₂is methyl. In other embodiments, R₁and R₂are methyl.
In some embodiments, x+y+z is at least 3. In other embodiments, x+y+z is 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10. In some embodiments, the sum of x+y+z is 3 or 6. In some embodiments, the sum of x+y+z is 3. In other embodiments, the sum of x+y+z is 6. Each occurrence of A, B, C, D or E in a macrocycle or macrocycle precursor is independently selected. For example, a sequence represented by the formula [A]_x, when x is 3, encompasses embodiments where the amino acids are not identical, e.g. Gln-Asp-Ala as well as embodiments where the amino acids are identical, e.g. Gln-Gln-Gln. This applies for any value of x, y, or z in the indicated ranges. Similarly, when u is greater than 1, each compound can encompass peptidomimetic macrocycles which are the same or different. For example, a compound can comprise peptidomimetic macrocycles comprising different linker lengths or chemical compositions.
In some embodiments, the peptidomimetic macrocycle comprises a secondary structure which is an α-helix and R₈is —H, allowing intrahelical hydrogen bonding. In some embodiments, at least one of A, B, C, D or E is an α,α-disubstituted amino acid. In one example, B is an α,α-disubstituted amino acid. For instance, at least one of A, B, C, D or E is 2-aminoisobutyric acid. In other embodiments, at least one of A, B, C, D or E is
In other embodiments, the length of the macrocycle-forming linker L as measured from a first Cα to a second Cα is selected to stabilize a desired secondary peptide structure, such as an α-helix formed by residues of the peptidomimetic macrocycle including, but not necessarily limited to, those between the first Cα to a second Cα.
In some embodiments, a peptidomimetic macrocycle of Formula (I) has Formula:
wherein:
each A, C, D, and E is independently a natural or non-natural amino acid;
each B is independently a natural or non-natural amino acid, amino acid analog,
[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];
each L is independently a macrocycle-forming linker;
each L′ is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene, each being optionally substituted with R₅, or a bond, or together with R₁and the atom to which both R₁and L′ are bound forms a ring;
each L″ is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene, each being optionally substituted with R₅, or a bond, or together with R₂and the atom to which both R₂and L″ are bound forms a ring;
each R₁is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-, or together with L′ and the atom to which both R₁and L′ are bound forms a ring;
each R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-, or together with L″ and the atom to which both R₂and L″ are bound forms a ring;
each R₃is independently hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, aryl, or heteroaryl, optionally substituted with R₅;
each L₃is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, heteroarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each n is independently an integer from 1-5;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
each v and w is independently an integer from 1-1000, for example 1-500, 1-200, 1-100, 1-50, 1-40, 1-25, 1-20, 1-15, or 1-10; and
each u, x, y and z is independently an integer from 0-10.
In some embodiments, the peptidomimetic macrocycles have the Formula I:
wherein:
each A, C, D, and E is independently a natural or non-natural amino acid;
each B is independently a natural or non-natural amino acid, amino acid analog,
[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];
each R₁and R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-;
each R₃is independently hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, aryl, or heteroaryl, optionally substituted with R₅;
each L is independently a macrocycle-forming linker of the formula
each L₁, L₂and L₃is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, heteroarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, optionally substituted with R₅, or part of a cyclic structure with a D residue;
each R₈is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, aryl, or heteroaryl, optionally substituted with R₅, or part of a cyclic structure with an E residue;
each v and w is independently an integer from 1-1000;
each u, x, y and z is independently integers from 0-10; and
n is an integer from 1-5.
In one embodiment, the peptidomimetic macrocycle of Formula (I) is:
wherein each R₁and R₂is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-.
In related embodiments, the peptidomimetic macrocycle of Formula (I) is:
wherein each R₁′ and R₂′ is independently an amino acid.
In other embodiments, the peptidomimetic macrocycle of Formula (I) is a compound of any of the formulas shown below:
wherein “AA” represents any natural or non-natural amino acid side chain and “
” is [D]_v, [E]_was defined above, and n is an integer between 0 and 20, 50, 100, 200, 300, 400 or 500. In some embodiments, n is 0. In other embodiments, n is less than 50.
Exemplary embodiments of the macrocycle-forming linker L are shown below.
In other embodiments, D and/or E in the compound of Formula I are further modified in order to facilitate cellular uptake. In some embodiments, lipidating or PEGylating a peptidomimetic macrocycle facilitates cellular uptake, increases bioavailability, increases blood circulation, alters pharmacokinetics, decreases immunogenicity and/or decreases the needed frequency of administration.
In other embodiments, at least one of [D] and [E] in the compound of Formula I represents a moiety comprising an additional macrocycle-forming linker such that the peptidomimetic macrocycle comprises at least two macrocycle-forming linkers. In a specific embodiment, a peptidomimetic macrocycle comprises two macrocycle-forming linkers. In an embodiment, u is 2.
In some embodiments, any of the macrocycle-forming linkers described herein can be used in any combination with any of the sequences shown in Table 1, Table 1a, Table 1b, and Table 1c and also with any of the R-substituents indicated herein.
In some embodiments, the peptidomimetic macrocycle comprises at least one α-helix motif. For example, A, B and/or C in the compound of Formula I include one or more α-helices. As a general matter, α-helices include between 3 and 4 amino acid residues per turn. In some embodiments, the α-helix of the peptidomimetic macrocycle includes 1 to 5 turns and, therefore, 3 to 20 amino acid residues. In specific embodiments, the α-helix includes 1 turn, 2 turns, 3 turns, 4 turns, or 5 turns. In some embodiments, the macrocycle-forming linker stabilizes an α-helix motif included within the peptidomimetic macrocycle. Thus, in some embodiments, the length of the macrocycle-forming linker L from a first Cα to a second Cα is selected to increase the stability of an α-helix. In some embodiments, the macrocycle-forming linker spans from 1 turn to 5 turns of the α-helix. In some embodiments, the macrocycle-forming linker spans approximately 1 turn, 2 turns, 3 turns, 4 turns, or 5 turns of the α-helix. In some embodiments, the length of the macrocycle-forming linker is approximately 5 Å to 9 Å per turn of the α-helix, or approximately 6 Å to 8 Å per turn of the α-helix. Where the macrocycle-forming linker spans approximately 1 turn of an α-helix, the length is equal to approximately 5 carbon-carbon bonds to 13 carbon-carbon bonds, approximately 7 carbon-carbon bonds to 11 carbon-carbon bonds, or approximately 9 carbon-carbon bonds. Where the macrocycle-forming linker spans approximately 2 turns of an α-helix, the length is equal to approximately 8 carbon-carbon bonds to 16 carbon-carbon bonds, approximately 10 carbon-carbon bonds to 14 carbon-carbon bonds, or approximately 12 carbon-carbon bonds. Where the macrocycle-forming linker spans approximately 3 turns of an α-helix, the length is equal to approximately 14 carbon-carbon bonds to 22 carbon-carbon bonds, approximately 16 carbon-carbon bonds to 20 carbon-carbon bonds, or approximately 18 carbon-carbon bonds. Where the macrocycle-forming linker spans approximately 4 turns of an α-helix, the length is equal to approximately 20 carbon-carbon bonds to 28 carbon-carbon bonds, approximately 22 carbon-carbon bonds to 26 carbon-carbon bonds, or approximately 24 carbon-carbon bonds. Where the macrocycle-forming linker spans approximately 5 turns of an α-helix, the length is equal to approximately 26 carbon-carbon bonds to 34 carbon-carbon bonds, approximately 28 carbon-carbon bonds to 32 carbon-carbon bonds, or approximately 30 carbon-carbon bonds. Where the macrocycle-forming linker spans approximately 1 turn of an α-helix, the linkage contains approximately 4 atoms to 12 atoms, approximately 6 atoms to 10 atoms, or approximately 8 atoms. Where the macrocycle-forming linker spans approximately 2 turns of the α-helix, the linkage contains approximately 7 atoms to 15 atoms, approximately 9 atoms to 13 atoms, or approximately 11 atoms. Where the macrocycle-forming linker spans approximately 3 turns of the α-helix, the linkage contains approximately 13 atoms to 21 atoms, approximately 15 atoms to 19 atoms, or approximately 17 atoms. Where the macrocycle-forming linker spans approximately 4 turns of the α-helix, the linkage contains approximately 19 atoms to 27 atoms, approximately 21 atoms to 25 atoms, or approximately 23 atoms. Where the macrocycle-forming linker spans approximately 5 turns of the α-helix, the linkage contains approximately 25 atoms to 33 atoms, approximately 27 atoms to 31 atoms, or approximately 29 atoms. Where the macrocycle-forming linker spans approximately 1 turn of the α-helix, the resulting macrocycle forms a ring containing approximately 17 members to 25 members, approximately 19 members to 23 members, or approximately 21 members. Where the macrocycle-forming linker spans approximately 2 turns of the α-helix, the resulting macrocycle forms a ring containing approximately 29 members to 37 members, approximately 31 members to 35 members, or approximately 33 members. Where the macrocycle-forming linker spans approximately 3 turns of the α-helix, the resulting macrocycle forms a ring containing approximately 44 members to 52 members, approximately 46 members to 50 members, or approximately 48 members. Where the macrocycle-forming linker spans approximately 4 turns of the α-helix, the resulting macrocycle forms a ring containing approximately 59 members to 67 members, approximately 61 members to 65 members, or approximately 63 members. Where the macrocycle-forming linker spans approximately 5 turns of the α-helix, the resulting macrocycle forms a ring containing approximately 74 members to 82 members, approximately 76 members to 80 members, or approximately 78 members.
In other embodiments, provided are peptidomimetic macrocycles of Formula (IV) or (IVa):
wherein:
each A, C, D, and E is independently a natural or non-natural amino acid, and the terminal D and E independently optionally include a capping group;
each B is independently a natural or non-natural amino acid, amino acid analog,
[—NH-L₃-CO—], [—NH-L₃-SO₂—], or [—NH-L₃-];
each R₁and R₂are independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, cycloalkylalkyl, heteroalkyl, or heterocycloalkyl, unsubstituted or substituted with halo-; or at least one of R₁and R₂forms a macrocycle-forming linker L′ connected to the alpha position of one of said D or E amino acids;
each R₃independently is hydrogen, alkyl, alkenyl, alkynyl, arylalkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, cycloalkylalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
each L independently is a macrocycle-forming linker of the formula -L₁-L₂-;
each L₁, L₂and L₃are independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, cycloarylene, heterocycloarylene, or [—R₄—K—R₄—]_n, each being optionally substituted with R₅;
each R₄is independently alkylene, alkenylene, alkynylene, heteroalkylene, cycloalkylene, heterocycloalkylene, arylene, or heteroarylene;
each K is independently O, S, SO, SO₂, CO, CO₂, or CONR₃;
each R₅is independently halogen, alkyl, —OR₆, —N(R₆)₂, —SR₆, —SOR₆, —SO₂R₆, —CO₂R₆, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₆is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heterocycloalkyl, a fluorescent moiety, a radioisotope or a therapeutic agent;
each R₇is independently —H, alkyl, alkenyl, alkynyl, arylalkyl, cycloalkyl, heteroalkyl, cycloalkylalkyl, heterocycloalkyl, cycloaryl, or heterocycloaryl, optionally substituted with R₅;
each v and w are independently integers from 1-1000;
u is an integer from 1-10;
each x, y and z are independently integers from 0-10; and
each n independently is an integer from 1-5.
In one example, L₁and L₂, either alone or in combination, do not form a triazole or a thioether.
In one example, at least one of R₁and R₂is alkyl, unsubstituted or substituted with halo-. In another example, both R₁and R₂are independently alkyl, unsubstituted or substituted with halo-. In some embodiments, at least one of R₁and R₂is methyl. In other embodiments, R₁and R₂are methyl.
In some embodiments, x+y+z is at least 1. In other embodiments, x+y+z is at least 2. In other embodiments, x+y+z is 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10. Each occurrence of A, B, C, D or E in a macrocycle or macrocycle precursor is independently selected. For example, a sequence represented by the formula [A]_x, when x is 3, encompasses embodiments where the amino acids are not identical, e.g. Gln-Asp-Ala as well as embodiments where the amino acids are identical, e.g. Gln-Gln-Gln. This applies for any value of x, y, or z in the indicated ranges.
In some embodiments, the peptidomimetic macrocycle comprises a secondary structure which is an α-helix and R₈is —H, allowing intrahelical hydrogen bonding. In some embodiments, at least one of A, B, C, D or E is an α,α-disubstituted amino acid. In one example, B is an α,α-disubstituted amino acid. For instance, at least one of A, B, C, D or E is 2-aminoisobutyric acid. In other embodiments, at least one of A, B, C, D or E is
In other embodiments, the length of the macrocycle-forming linker L as measured from a first Cα to a second Cα is selected to stabilize a desired secondary peptide structure, such as an α-helix formed by residues of the peptidomimetic macrocycle including, but not necessarily limited to, those between the first Cα to a second Cα.
Exemplary embodiments of the macrocycle-forming linker -L₁-L₂- are shown below.
In some embodiments, L is a macrocycle-forming linker of the formula
Exemplary embodiments of such macrocycle-forming linkers L are shown below.
Unless otherwise stated, any compounds (including peptidomimetic macrocycles, macrocycle precursors, and other compositions) are also meant to encompass compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the described structures except for the replacement of a hydrogen by a deuterium or tritium, or the replacement of a carbon by ¹³C- or ¹⁴C-enriched carbon are within the scope of this disclosure.
In some embodiments, the compounds disclosed herein can contain unnatural proportions of atomic isotopes at one or more of atoms that constitute such compounds. For example, the compounds can be radiolabeled with radioactive isotopes, such as for example tritium (³H), iodine-125 (¹²⁵I) or carbon-14 (¹⁴C). In other embodiments, one or more carbon atoms is replaced with a silicon atom. The compounds (including peptidomimetic macrocycles, macrocycle precursors, and other compositions) also include salts thereof. For example, salts of acidic and basic amino acids. All isotopic variations of the compounds disclosed herein, whether radioactive or not, are contemplated herein.
The compound or peptidomimetic macrocycles described herein can be at least 1% pure, at least 2% pure, at least 3% pure, at least 4% pure, at least 5% pure, at least 6% pure, at least 7% pure, at least 8% pure, at least 9% pure, at least 10% pure, at least 11% pure, at least 12% pure, at least 13% pure, at least 14% pure, at least 15% pure, at least 16% pure, at least 17% pure, at least 18% pure, at least 19% pure, at least 20% pure, at least 21% pure, at least 22% pure, at least 23% pure, at least 24% pure, at least 25% pure, at least 26% pure, at least 27% pure, at least 28% pure, at least 29% pure, at least 30% pure, at least 31% pure, at least 32% pure, at least 33% pure, at least 34% pure, at least 35% pure, at least 36% pure, at least 37% pure, at least 38% pure, at least 39% pure, at least 40% pure, at least 41% pure, at least 42% pure, at least 43% pure, at least 44% pure, at least 45% pure, at least 46% pure, at least 47% pure, at least 48% pure, at least 49% pure, at least 50% pure, at least 51% pure, at least 52% pure, at least 53% pure, at least 54% pure, at least 55% pure, at least 56% pure, at least 57% pure, at least 58% pure, at least 59% pure, at least 60% pure, at least 61% pure, at least 62% pure, at least 63% pure, at least 64% pure, at least 65% pure, at least 66% pure, at least 67% pure, at least 68% pure, at least 69% pure, at least 70% pure, at least 71% pure, at least 72% pure, at least 73% pure, at least 74% pure, at least 75% pure, at least 76% pure, at least 77% pure, at least 78% pure, at least 79% pure, at least 80% pure, at least 81% pure, at least 82% pure, at least 83% pure, at least 84% pure, at least 85% pure, at least 86% pure, at least 87% pure, at least 88% pure, at least 89% pure, at least 90% pure, at least 91% pure, at least 92% pure, at least 93% pure, at least 94% pure, at least 95% pure, at least 96% pure, at least 97% pure, at least 98% pure, at least 99% pure, at least 99.1% pure, at least 99.2% pure, at least 99.3% pure, at least 99.4% pure, at least 99.5% pure, at least 99.6% pure, at least 99.7% pure, at least 99.8% pure, or at least 99.9% pure on a chemical, optical, isomeric, enantiomeric, or diastereomeric basis. Purity can be assessed, for example, by HPLC, MS, LC/MS, melting point, or NMR.
Two or more peptides can share a degree of homology. A pair of peptides can have, for example, up to about 20% pairwise homology, up to about 25% pairwise homology, up to about 30% pairwise homology, up to about 35% pairwise homology, up to about 40% pairwise homology, up to about 45% pairwise homology, up to about 50% pairwise homology, up to about 55% pairwise homology, up to about 60% pairwise homology, up to about 65% pairwise homology, up to about 70% pairwise homology, up to about 75% pairwise homology, up to about 80% pairwise homology, up to about 85% pairwise homology, up to about 90% pairwise homology, up to about 95% pairwise homology, up to about 96% pairwise homology, up to about 97% pairwise homology, up to about 98% pairwise homology, up to about 99% pairwise homology, up to about 99.5% pairwise homology, or up to about 99.9% pairwise homology. A pair of peptides can have, for example, at least about 20% pairwise homology, at least about 25% pairwise homology, at least about 30% pairwise homology, at least about 35% pairwise homology, at least about 40% pairwise homology, at least about 45% pairwise homology, at least about 50% pairwise homology, at least about 55% pairwise homology, at least about 60% pairwise homology, at least about 65% pairwise homology, at least about 70% pairwise homology, at least about 75% pairwise homology, at least about 80% pairwise homology, at least about 85% pairwise homology, at least about 90% pairwise homology, at least about 95% pairwise homology, at least about 96% pairwise homology, at least about 97% pairwise homology, at least about 98% pairwise homology, at least about 99% pairwise homology, at least about 99.5% pairwise homology, at least about 99.9% pairwise homology.
Various methods and software programs can be used to determine the homology between two or more peptides, such as NCBI BLAST, Clustal W, MAFFT, Clustal Omega, AlignMe, Praline, or another suitable method or algorithm.
The circulating half-life of the peptidomimetic macrocycles in human blood can be about 1-24 h. For example the circulating half-life of the peptidomimetic macrocycles in human blood can me about 2-24 h, 4-24 h, 6-24 h, 8-24 h, 10-24 h, 12-24 h, 14-24 h, 16-24 h, 18-24 h, 20-24 h, 22-24 h, 1-20 h, 4-20 h, 6-20 h, 8-20 h, 10-20 h, 12-20 h, 14-20 h, 16-20 h, 18-20 h, 1-16 h, 4-16 h, 6-16 h, 8-16 h, 10-16 h, 12-16 h, 14-16 h, 1-12 h, 4-12 h, 6-12 h, 8-12 h, 10-12 h, 1-8 h, 4-8 h, 6-8 h, or 1-4 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood can be bout 1-12 h, for example about 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, or 12 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 2 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 4 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 6 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 8 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 10 h.
The half-life of the peptidomimetic macrocycles in biological tissue can be about 1-24 h. For example the circulating half-life of the peptidomimetic macrocycles in human blood can me about 1-24 h, 5-24 h, 10-24 h, 15-24 h, 20-24 h, 1-22 h, 5-22 h, 10-22 h, 15-22 h, 20-22 h, 1-20 h, 5-20 h, 15-20 h, 1-18 h, 5-18 h, 10-18 h, 15-18 h, 1-16 h, 5-16 h, 10-16 h, 15-16 h, 1-14 h, 5-14 h, 10-14 h, 1-12 h, 5-12 h, 10-12 h, 1-10 h, 5-10 h, 1-8 h, 5-8 h, 1-6 h, 5-6 h, or 1-4 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood can be bout 5-20 h, for example about 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, 12 h, 13 h, 14 h, 15 h, 16 h, 17 h, 18 h, 19 h or 20 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 2 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 4 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 6 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 8 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 10 h.
The circulating half-life of the peptidomimetic macrocycles in human blood can be greater than, equal to, or less than the half-life of the peptidomimetic macrocycles in biological tissue. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood can be greater than the half-life of the peptidomimetic macrocycles in biological tissue. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood can be equal to the half-life of the peptidomimetic macrocycles in biological tissue. In some examples, the half-life of the peptidomimetic macrocycles in biological tissue is greater than the circulating half-life of the peptidomimetic macrocycles in human blood. This can facilitate administration of the peptidomimetic macrocycles at a lower dose and/or at lower frequency. In some embodiments, the half-life of the peptidomimetic macrocycles in biological tissue is at least 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, or 12 h greater than the than the circulating half-life of the peptidomimetic macrocycles in human blood. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 4 h and the half-life of the in biological tissue is about 10 h. In some examples, the circulating half-life of the peptidomimetic macrocycles in human blood is about 6 h and the half-life of the in biological tissue is about 10 h.
The cross-linked peptides of the disclosure can be modeled after the N-terminal transactivation domain of p53 (“p⁵³peptidomimetic macrocycles”). These cross-linked peptides contain at least two modified amino acids that together form an intramolecular cross-link that can help to stabilize the α-helical secondary structure of a portion of p53 that is thought to be important for binding of p53 to MDM2 and for binding of p53 to MDMX. Accordingly, a cross-linked polypeptide described herein can have improved biological activity relative to a corresponding polypeptide that is not cross-linked. The p53 peptidomimetic macrocycles are thought to interfere with binding of p53 to MDM2 and/or of p53 to MDMX, thereby liberating functional p53 and inhibiting its destruction. The p53 peptidomimetic macrocycles described herein can be used therapeutically, for example to treat cancers and other disorders characterized by an undesirably low level or a low activity of p53, and/or to treat cancers and other disorders characterized by an undesirably high level of activity of MDM2 or MDMX. The p53 peptidomimetic macrocycles can also be useful for treatment of any disorder associated with disrupted regulation of the p53 transcriptional pathway, leading to conditions of excess cell survival and proliferation such as cancer and autoimmunity, in addition to conditions of inappropriate cell cycle arrest and apoptosis such as neurodegeneration and immune deficiencies. In some embodiments, the p53 peptidomimetic macrocycles bind to MDM2 (e.g., GenBank® Accession No.: 228952; GI:228952) and/or MDMX (also referred to as MDM4; GenBank® Accession No.: 88702791; GI:88702791).
Table 1 shows a list of peptidomimetic macrocycles derived from the MDM2/MDMX-binding helix of p53 that were prepared. Tables 1a, 1b, 1c, 1d and 1e show a list of selected peptidomimetic macrocycles from Table 1. Table if shows a list of selected peptidomimetic macrocycles from Table 1e. A partial staple scan was performed on the linear peptide p-CF₃-Phe7-D-PMI-β. SP-757, a potent and selective MDM2 antagonist, was prepared by including an i, i+7 crosslink to the sequence of p-CF3-Phe-7-D-PMI-β. SP-757 exhibited SJSA-1 sarcoma cell killing activity at a single digit micromolar concentration (EC₅₀=1.5 mM). (FIG. 3). SP-763 was prepared by increasing the alanine content to 35% while maintaining the net charge and Von Heijne score by adding four alanine residues to the C-terminus of SP-757. SP-763 exhibited improved SJSA-1 sarcoma cell killing activity (EC₅₀=0.15 mM) compared to SP-757 (FIG. 3). SP-763 exhibited similar cell killing activity as SP-449.

TABLE 1

List of peptidomimetic macrocycles derived from the MDM2/MDMX-binding helix of p53 that were prepared.

	SEQ ID			Exact	Found	Calc	Calc	Calc
SP	NO:	Sequence	Isomer	Mass	Mass	(M + 1)/1	(M + 2)/2	(M + 3)/3

SP1	9	Ac-F$r8AYWEAc3cL$AAA-NH2		1456.78	729.44	1457.79	729.4	486.6
SP2	10	Ac-F$r8AYWEAc3cL$AAibA-NH2		1470.79	736.4	1471.8	736.4	491.27
SP3	11	Ac-LTF$r8AYWAQL$SANle-NH2		1715.97	859.02	1716.98	858.99	573
SP4	12	Ac-LTF$r8AYWAQL$SAL-NH2		1715.97	859.02	1716.98	858.99	573
SP5	13	Ac-LTF$r8AYWAQL$SAM-NH2		1733.92	868.48	1734.93	867.97	578.98
SP6	14	Ac-LTF$r8AYWAQL$SAhL-NH2		1729.98	865.98	1730.99	866	577.67
SP7	15	Ac-LTF$r8AYWAQL$SAF-NH2		1749.95	876.36	1750.96	875.98	584.32
SP8	16	Ac-LTF$r8AYWAQL$SAI-NH2		1715.97	859.02	1716.98	858.99	573
SP9	17	Ac-LTF$r8AYWAQL$SAChg-NH2		1741.98	871.98	1742.99	872	581.67
SP10	18	Ac-LTF$r8AYWAQL$SAAib-NH2		1687.93	845.36	1688.94	844.97	563.65
SP11	19	Ac-LTF$r8AYWAQL$SAA-NH2		1673.92	838.01	1674.93	837.97	558.98
SP12	20	Ac-LTF$r8AYWA$L$S$Nle-NH2		1767.04	884.77	1768.05	884.53	590.02
SP13	21	Ac-LTF$r8AYWA$L$S$A-NH2		1724.99	864.23	1726	863.5	576
SP14	22	Ac-F$r8AYWEAc3cL$AANle-NH2		1498.82	750.46	1499.83	750.42	500.61
SP15	23	Ac-F$r8AYWEAc3cL$AAL-NH2		1498.82	750.46	1499.83	750.42	500.61
SP16	24	Ac-F$r8AYWEAc3cL$AAM-NH2		1516.78	759.41	1517.79	759.4	506.6
SP17	25	Ac-F$r8AYWEAc3cL$AAhL-NH2		1512.84	757.49	1513.85	757.43	505.29
SP18	26	Ac-F$r8AYWEAc3cL$AAF-NH2		1532.81	767.48	1533.82	767.41	511.94
SP19	27	Ac-F$r8AYWEAc3cL$AAI-NH2		1498.82	750.39	1499.83	750.42	500.61
SP20	28	Ac-F$r8AYWEAc3cL$AAChg-NH2		1524.84	763.48	1525.85	763.43	509.29
SP21	29	Ac-F$r8AYWEAc3cL$AACha-NH2		1538.85	770.44	1539.86	770.43	513.96
SP22	30	Ac-F$r8AYWEAc3cL$AAAib-NH2		1470.79	736.84	1471.8	736.4	491.27
SP23	31	Ac-LTF$r8AYWAQL$AAAibV-NH2		1771.01	885.81	1772.02	886.51	591.34
SP24	32	Ac-LTF$r8AYWAQL$AAAibV-NH2	iso2	1771.01	886.26	1772.02	886.51	591.34
SP25	33	Ac-LTF$r8AYWAQL$SAibAA-NH2		1758.97	879.89	1759.98	880.49	587.33
SP26	34	Ac-LTF$r8AYWAQL$SAibAA-NH2	iso2	1758.97	880.34	1759.98	880.49	587.33
SP27	35	Ac-HLTF$r8MWHQL$AANleNle-		2056.15	1028.86	2057.16	1029.08	686.39
		NH2
SP28	36	Ac-DLTF$r8MWHQL$RRLV-NH2		2190.23	731.15	2191.24	1096.12	731.08
SP29	37	Ac-HHTF$r8MWHQL$AAML-NH2		2098.08	700.43	2099.09	1050.05	700.37
SP30	38	Ac-F$r8MWHQL$RRDCha-NH2		1917.06	959.96	1918.07	959.54	640.03
SP31	39	Ac-F$r8MWHQL$HRFV-NH2		1876.02	938.65	1877.03	939.02	626.35
SP32	40	Ac-HLTF$r8MWHQL$AAhLA-NH2		2028.12	677.2	2029.13	1015.07	677.05
SP33	41	Ac-DLTF$r8MWHQL$RRChg1-NH2		2230.26	1115.89	2231.27	1116.14	744.43
SP34	42	Ac-DLTF$r8MWHQL$RRChg1-NH2	iso2	2230.26	1115.96	2231.27	1116.14	744.43
SP35	43	Ac-HHTF$r8MWHQL$AAChav-NH2		2106.14	1053.95	2107.15	1054.08	703.05
SP36	44	Ac-F$r8MWHQL$RRDa-NH2		1834.99	918.3	1836	918.5	612.67
SP37	45	Ac-F$r8MWHQL$HRAibG-NH2		1771.95	886.77	1772.96	886.98	591.66
SP38	46	Ac-F$r8AYWAQL$HHNleL-NH2		1730.97	866.57	1731.98	866.49	578
SP39	47	Ac-F$r8AYWSAL$HQANle-NH2		1638.89	820.54	1639.9	820.45	547.3
SP40	48	Ac-F$r8AYWVQL$QHChg1-NH2		1776.01	889.44	1777.02	889.01	593.01
SP41	49	Ac-F$r8AYWTAL$QQNlev-NH2		1671.94	836.97	1672.95	836.98	558.32
SP42	50	Ac-F$r8AYWYQL$HAibAa-NH2		1686.89	844.52	1687.9	844.45	563.3
SP43	51	Ac-LTF$r8AYWAQL$HHLa-NH2		1903.05	952.27	1904.06	952.53	635.36
SP44	52	Ac-LTF$r8AYWAQL$HHLa-NH2	iso2	1903.05	952.27	1904.06	952.53	635.36
SP45	53	Ac-LTF$r8AYWAQL$HQNlev-NH2		1922.08	962.48	1923.09	962.05	641.7
SP46	54	Ac-LTF$r8AYWAQL$HQNlev-NH2	iso2	1922.08	962.4	1923.09	962.05	641.7
SP47	55	Ac-LTF$r8AYWAQL$QQM1-NH2		1945.05	973.95	1946.06	973.53	649.36
SP48	56	Ac-LTF$r8AYWAQL$QQM1-NH2	iso2	1945.05	973.88	1946.06	973.53	649.36
SP49	57	Ac-LTF$r8AYWAQL$HAibhLV-NH2		1893.09	948.31	1894.1	947.55	632.04
SP50	58	Ac-LTF$r8AYWAQL$AHFA-NH2		1871.01	937.4	1872.02	936.51	624.68
SP51	59	Ac-HLTF$r8MWHQL$AANle1-NH2		2056.15	1028.79	2057.16	1029.08	686.39
SP52	60	Ac-DLTF$r8MWHQL$RRLa-NH2		2162.2	721.82	2163.21	1082.11	721.74
SP53	61	Ac-HHTF$r8MWHQL$AAMv-NH2		2084.07	1042.92	2085.08	1043.04	695.7
SP54	62	Ac-F$r8MWHQL$RRDA-NH2		1834.99	612.74	1836	918.5	612.67
SP55	63	Ac-F$r8MWHQL$HRFCha-NH2		1930.06	966.47	1931.07	966.04	644.36
SP56	64	Ac-F$r8AYWEAL$AA-NHAm		1443.82	1445.71	1444.83	722.92	482.28
SP57	65	Ac-F$r8AYWEAL$AA-NHiAm		1443.82	723.13	1444.83	722.92	482.28
SP58	66	Ac-F$r8AYWEAL$AA-NHnPr3Ph		1491.82	747.3	1492.83	746.92	498.28
SP59	67	Ac-F$r8AYWEAL$AA-NHnBu33Me		1457.83	1458.94	1458.84	729.92	486.95
SP60	68	Ac-F$r8AYWEAL$AA-NHnPr		1415.79	709.28	1416.8	708.9	472.94
SP61	69	Ac-F$r8AYWEAL$AA-NHnEt2Ch		1483.85	1485.77	1484.86	742.93	495.62
SP62	70	Ac-F$r8AYWEAL$AA-NHnEt2Cp		1469.83	1470.78	1470.84	735.92	490.95
SP63	71	Ac-F$r8AYWEAL$AA-NHHex		1457.83	730.19	1458.84	729.92	486.95
SP64	72	Ac-LTF$r8AYWAQL$AAIA-NH2		1771.01	885.81	1772.02	886.51	591.34
SP65	73	Ac-LTF$r8AYWAQL$AAIA-NH2	iso2	1771.01	866.8	1772.02	886.51	591.34
SP66	74	Ac-LTF$r8AYWAAL$AAMA-NH2		1731.94	867.08	1732.95	866.98	578.32
SP67	75	Ac-LTF$r8AYWAAL$AAMA-NH2	iso2	1731.94	867.28	1732.95	866.98	578.32
SP68	76	Ac-LTF$r8AYWAQL$AANleA-NH2		1771.01	867.1	1772.02	886.51	591.34
SP69	77	Ac-LTF$r8AYWAQL$AANleA-NH2	iso2	1771.01	886.89	1772.02	886.51	591.34
SP70	78	Ac-LTF$r8AYWAQL$AAIa-NH2		1771.01	886.8	1772.02	886.51	591.34
SP71	79	Ac-LTF$r8AYWAQL$AAIa-NH2	iso2	1771.01	887.09	1772.02	886.51	591.34
SP72	80	Ac-LTF$r8AYWAAL$AAMa-NH2		1731.94	867.17	1732.95	866.98	578.32
SP73	81	Ac-LTF$r8AYWAAL$AAMa-NH2	iso2	1731.94	867.37	1732.95	866.98	578.32
SP74	82	Ac-LTF$r8AYWAQL$AANlea-NH2		1771.01	887.08	1772.02	886.51	591.34
SP75	83	Ac-LTF$r8AYWAQL$AANlea-NH2	iso2	1771.01	887.08	1772.02	886.51	591.34
SP76	84	Ac-LTF$r8AYWAAL$AAIv-NH2		1742.02	872.37	1743.03	872.02	581.68
SP77	85	Ac-LTF$r8AYWAAL$AAIv-NH2	iso2	1742.02	872.74	1743.03	872.02	581.68
SP78	86	Ac-LTF$r8AYWAQL$AAMv-NH2		1817	910.02	1818.01	909.51	606.67
SP79	87	Ac-LTF$r8AYWAAL$AANlev-NH2		1742.02	872.37	1743.03	872.02	581.68
SP80	88	Ac-LTF$r8AYWAAL$AANlev-NH2	iso2	1742.02	872.28	1743.03	872.02	581.68
SP81	89	Ac-LTF$r8AYWAQL$AAI1-NH2		1813.05	907.81	1814.06	907.53	605.36
SP82	90	Ac-LTF$r8AYWAQL$AAI1-NH2	iso2	1813.05	907.81	1814.06	907.53	605.36
SP83	91	Ac-LTF$r8AYWAAL$AAM1-NH2		1773.99	887.37	1775	888	592.34
SP84	92	Ac-LTF$r8AYWAQL$AANle1-NH2		1813.05	907.61	1814.06	907.53	605.36
SP85	93	Ac-LTF$r8AYWAQL$AANle1-NH2	iso2	1813.05	907.71	1814.06	907.53	605.36
SP86	94	Ac-F$r8AYWEAL$AAMA-NH2		1575.82	789.02	1576.83	788.92	526.28
SP87	95	Ac-F$r8AYWEAL$AANleA-NH2		1557.86	780.14	1558.87	779.94	520.29
SP88	96	Ac-F$r8AYWEAL$AAIa-NH2		1557.86	780.33	1558.87	779.94	520.29
SP89	97	Ac-F$r8AYWEAL$AAMa-NH2		1575.82	789.3	1576.83	788.92	526.28
SP90	98	Ac-F$r8AYWEAL$AANlea-NH2		1557.86	779.4	1558.87	779.94	520.29
SP91	99	Ac-F$r8AYWEAL$AAIv-NH2		1585.89	794.29	1586.9	793.95	529.64
SP92	100	Ac-F$r8AYWEAL$AAMv-NH2		1603.85	803.08	1604.86	802.93	535.62
SP93	101	Ac-F$r8AYWEAL$AANlev-NH2		1585.89	793.46	1586.9	793.95	529.64
SP94	102	Ac-F$r8AYWEAL$AAI1-NH2		1599.91	800.49	1600.92	800.96	534.31
SP95	103	Ac-F$r8AYWEAL$AAM1-NH2		1617.86	809.44	1618.87	809.94	540.29
SP96	104	Ac-F$r8AYWEAL$AANle1-NH2		1599.91	801.7	1600.92	800.96	534.31
SP97	105	Ac-F$r8AYWEAL$AANle1-NH2	iso2	1599.91	801.42	1600.92	800.96	534.31
SP98	106	Ac-LTF$r8AY6clWAQL$SAA-NH2		1707.88	855.72	1708.89	854.95	570.3
SP99	107	Ac-LTF$r8AY6clWAQL$SAA-NH2	iso2	1707.88	855.35	1708.89	854.95	570.3
SP100	108	Ac-WTF$r8FYWSQL$AVAa-NH2		1922.01	962.21	1923.02	962.01	641.68
SP101	109	Ac-WTF$r8FYWSQL$AVAa-NH2	iso2	1922.01	962.49	1923.02	962.01	641.68
SP102	110	Ac-WTF$r8VYWSQL$AVA-NH2		1802.98	902.72	1803.99	902.5	602
SP103	111	Ac-WTF$r8VYWSQL$AVA-NH2	iso2	1802.98	903	1803.99	902.5	602
SP104	112	Ac-WTF$r8FYWSQL$SAAa-NH2		1909.98	956.47	1910.99	956	637.67
SP105	113	Ac-WTF$r8FYWSQL$SAAa-NH2	iso2	1909.98	956.47	1910.99	956	637.67
SP106	114	Ac-WTF$r8VYWSQL$AVAaa-NH2		1945.05	974.15	1946.06	973.53	649.36
SP107	115	Ac-WTF$r8VYWSQL$AVAaa-NH2	iso2	1945.05	973.78	1946.06	973.53	649.36
SP108	116	Ac-LTF$r8AYWAQL$AVG-NH2		1671.94	837.52	1672.95	836.98	558.32
SP109	117	Ac-LTF$r8AYWAQL$AVG-NH2	iso2	1671.94	837.21	1672.95	836.98	558.32
SP110	118	Ac-LTF$r8AYWAQL$AVQ-NH2		1742.98	872.74	1743.99	872.5	582
SP111	119	Ac-LTF$r8AYWAQL$AVQ-NH2	iso2	1742.98	872.74	1743.99	872.5	582
SP112	120	Ac-LTF$r8AYWAQL$SAa-NH2		1673.92	838.23	1674.93	837.97	558.98
SP113	121	Ac-LTF$r8AYWAQL$SAa-NH2	iso2	1673.92	838.32	1674.93	837.97	558.98
SP114	122	Ac-LTF$r8AYWAQhL$SAA-NH2		1687.93	844.37	1688.94	844.97	563.65
SP115	123	Ac-LTF$r8AYWAQhL$SAA-NH2	iso2	1687.93	844.81	1688.94	844.97	563.65
SP116	124	Ac-LTF$r8AYWEQLStSA$-NH2		1826	905.27	1827.01	914.01	609.67
SP117	125	Ac-LTF$r8AYWAQL$SLA-NH2		1715.97	858.48	1716.98	858.99	573
SP118	126	Ac-LTF$r8AYWAQL$SLA-NH2	iso2	1715.97	858.87	1716.98	858.99	573
SP119	127	Ac-LTF$r8AYWAQL$SWA-NH2		1788.96	895.21	1789.97	895.49	597.33
SP120	128	Ac-LTF$r8AYWAQL$SWA-NH2	iso2	1788.96	895.28	1789.97	895.49	597.33
SP121	129	Ac-LTF$r8AYWAQL$SVS-NH2		1717.94	859.84	1718.95	859.98	573.65
SP122	130	Ac-LTF$r8AYWAQL$SAS-NH2		1689.91	845.85	1690.92	845.96	564.31
SP123	131	Ac-LTF$r8AYWAQL$SVG-NH2		1687.93	844.81	1688.94	844.97	563.65
SP124	132	Ac-ETF$r8VYWAQL$SAa-NH2		1717.91	859.76	1718.92	859.96	573.64
SP125	133	Ac-ETF$r8VYWAQL$SAA-NH2		1717.91	859.84	1718.92	859.96	573.64
SP126	134	Ac-ETF$r8VYWAQL$SVA-NH2		1745.94	873.82	1746.95	873.98	582.99
SP127	135	Ac-ETF$r8VYWAQL$SLA-NH2		1759.96	880.85	1760.97	880.99	587.66
SP128	136	Ac-ETF$r8VYWAQL$SWA-NH2		1832.95	917.34	1833.96	917.48	611.99
SP129	137	Ac-ETF$r8KYWAQL$SWA-NH2		1861.98	931.92	1862.99	932	621.67
SP130	138	Ac-ETF$r8VYWAQL$SVS-NH2		1761.93	881.89	1762.94	881.97	588.32
SP131	139	Ac-ETF$r8VYWAQL$SAS-NH2		1733.9	867.83	1734.91	867.96	578.97
SP132	140	Ac-ETF$r8VYWAQL$SVG-NH2		1731.92	866.87	1732.93	866.97	578.31
SP133	141	Ac-LTF$r8VYWAQL$SSa-NH2		1717.94	859.47	1718.95	859.98	573.65
SP134	142	Ac-ETF$r8VYWAQL$SSa-NH2		1733.9	867.83	1734.91	867.96	578.97
SP135	143	Ac-LTF$r8VYWAQL$SNa-NH2		1744.96	873.38	1745.97	873.49	582.66
SP136	144	Ac-ETF$r8VYWAQL$SNa-NH2		1760.91	881.3	1761.92	881.46	587.98
SP137	145	Ac-LTF$r8VYWAQL$SAa-NH2		1701.95	851.84	1702.96	851.98	568.32
SP138	146	Ac-LTF$r8VYWAQL$SVA-NH2		1729.98	865.53	1730.99	866	577.67
SP139	147	Ac-LTF$r8VYWAQL$SVA-NH2	iso2	1729.98	865.9	1730.99	866	577.67
SP140	148	Ac-LTF$r8VYWAQL$SWA-NH2		1816.99	909.42	1818	909.5	606.67
SP141	149	Ac-LTF$r8VYWAQL$SVS-NH2		1745.98	873.9	1746.99	874	583
SP142	150	Ac-LTF$r8VYWAQL$SVS-NH2	iso2	1745.98	873.9	1746.99	874	583
SP143	151	Ac-LTF$r8VYWAQL$SAS-NH2		1717.94	859.84	1718.95	859.98	573.65
SP144	152	Ac-LTF$r8VYWAQL$SAS-NH2	iso2	1717.94	859.91	1718.95	859.98	573.65
SP145	153	Ac-LTF$r8VYWAQL$SVG-NH2		1715.97	858.87	1716.98	858.99	573
SP146	154	Ac-LTF$r8VYWAQL$SVG-NH2	iso2	1715.97	858.87	1716.98	858.99	573
SP147	155	Ac-LTF$r8EYWAQCha$SAA-NH2		1771.96	886.85	1772.97	886.99	591.66
SP148	156	Ac-LTF$r8EYWAQCha$SAA-NH2	iso2	1771.96	886.85	1772.97	886.99	591.66
SP149	157	Ac-LTF$r8EYWAQCpg$SAA-NH2		1743.92	872.86	1744.93	872.97	582.31
SP150	158	Ac-LTF$r8EYWAQCpg$SAA-NH2	iso2	1743.92	872.86	1744.93	872.97	582.31
SP151	159	Ac-LTF$r8EYWAQF$SAA-NH2		1765.91	883.44	1766.92	883.96	589.64
SP152	160	Ac-LTF$r8EYWAQF$SAA-NH2	iso2	1765.91	883.89	1766.92	883.96	589.64
SP153	161	Ac-LTF$r8EYWAQCba$SAA-NH2		1743.92	872.42	1744.93	872.97	582.31
SP154	162	Ac-LTF$r8EYWAQCba$SAA-NH2	iso2	1743.92	873.39	1744.93	872.97	582.31
SP155	163	Ac-LTF3C1$r8EYWAQL$SAA-NH2		1765.89	883.89	1766.9	883.95	589.64
SP156	164	Ac-LTF3C1$r8EYWAQL$SAA-NH2	iso2	1765.89	883.96	1766.9	883.95	589.64
SP157	165	Ac-LTF34F2$r8EYWAQL$SAA-NH2		1767.91	884.48	1768.92	884.96	590.31
SP158	166	Ac-LTF34F2$r8EYWAQL$SAA-NH2	iso2	1767.91	884.48	1768.92	884.96	590.31
SP159	167	Ac-LTF34F2$r8EYWAQhL$SAA-		1781.92	891.44	1782.93	891.97	594.98
		NH2
SP160	168	Ac-LTF34F2$r8EYWAQhL$SAA-	iso2	1781.92	891.88	1782.93	891.97	594.98
		NH2
SP161	169	Ac-ETF$r8EYWAQL$SAA-NH2		1747.88	874.34	1748.89	874.95	583.63
SP162	170	Ac-LTF$r8AYWVQL$SAA-NH2		1701.95	851.4	1702.96	851.98	568.32
SP163	171	Ac-LTF$r8AHWAQL$SAA-NH2		1647.91	824.83	1648.92	824.96	550.31
SP164	172	Ac-LTF$r8AEWAQL$SAA-NH2		1639.9	820.39	1640.91	820.96	547.64
SP165	173	Ac-LTF$r8ASWAQL$SAA-NH2		1597.89	799.38	1598.9	799.95	533.64
SP166	174	Ac-LTF$r8AEWAQL$SAA-NH2	iso2	1639.9	820.39	1640.91	820.96	547.64
SP167	175	Ac-LTF$r8ASWAQL$SAA-NH2	iso2	1597.89	800.31	1598.9	799.95	533.64
SP168	176	Ac-LTF$r8AF4coohWAQL$SAA-NH2		1701.91	851.4	1702.92	851.96	568.31
SP169	177	Ac-LTF$r8AF4coohWAQL$SAA-NH2	iso2	1701.91	851.4	1702.92	851.96	568.31
SP170	178	Ac-LTF$r8AHWAQL$AAIa-NH2		1745	874.13	1746.01	873.51	582.67
SP171	179	Ac-ITF$r8FYWAQL$AAIa-NH2		1847.04	923.92	1848.05	924.53	616.69
SP172	180	Ac-ITF$r8EHWAQL$AAIa-NH2		1803.01	903.17	1804.02	902.51	602.01
SP173	181	Ac-ITF$r8EHWAQL$AAIa-NH2	iso2	1803.01	903.17	1804.02	902.51	602.01
SP174	182	Ac-ETF$r8EHWAQL$AAIa-NH2		1818.97	910.76	1819.98	910.49	607.33
SP175	183	Ac-ETF$r8EHWAQL$AAIa-NH2	iso2	1818.97	910.85	1819.98	910.49	607.33
SP176	184	Ac-LTF$r8AHWVQL$AAIa-NH2		1773.03	888.09	1774.04	887.52	592.02
SP177	185	Ac-ITF$r8FYWVQL$AAIa-NH2		1875.07	939.16	1876.08	938.54	626.03
SP178	186	Ac-ITF$r8EYWVQL$AAIa-NH2		1857.04	929.83	1858.05	929.53	620.02
SP179	187	Ac-ITF$r8EHWVQL$AAIa-NH2		1831.04	916.86	1832.05	916.53	611.35
SP180	188	Ac-LTF$r8AEWAQL$AAIa-NH2		1736.99	869.87	1738	869.5	580
SP181	189	Ac-LTF$r8AF4coohWAQL$AAIa-		1799	900.17	1800.01	900.51	600.67
		NH2
SP182	190	Ac-LTF$r8AF4coohWAQL$AAIa-	iso2	1799	900.24	1800.01	900.51	600.67
		NH2
SP183	191	Ac-LTF$r8AHWAQL$AHFA-NH2		1845.01	923.89	1846.02	923.51	616.01
SP184	192	Ac-ITF$r8FYWAQL$AHFA-NH2		1947.05	975.05	1948.06	974.53	650.02
SP185	193	Ac-ITF$r8FYWAQL$AHFA-NH2	iso2	1947.05	976.07	1948.06	974.53	650.02
SP186	194	Ac-ITF$r8FHWAQL$AEFA-NH2		1913.02	958.12	1914.03	957.52	638.68
SP187	195	Ac-ITF$r8FHWAQL$AEFA-NH2	iso2	1913.02	957.86	1914.03	957.52	638.68
SP188	196	Ac-ITF$r8EHWAQL$AHFA-NH2		1903.01	952.94	1904.02	952.51	635.34
SP189	197	Ac-ITF$r8EHWAQL$AHFA-NH2	iso2	1903.01	953.87	1904.02	952.51	635.34
SP190	198	Ac-LTF$r8AHWVQL$AHFA-NH2		1873.04	937.86	1874.05	937.53	625.35
SP191	199	Ac-ITF$r8FYWVQL$AHFA-NH2		1975.08	988.83	1976.09	988.55	659.37
SP192	200	Ac-ITF$r8EYWVQL$AHFA-NH2		1957.05	979.35	1958.06	979.53	653.36
SP193	201	Ac-ITF$r8EHWVQL$AHFA-NH2		1931.05	967	1932.06	966.53	644.69
SP194	202	Ac-ITF$r8EHWVQL$AHFA-NH2	iso2	1931.05	967.93	1932.06	966.53	644.69
SP195	203	Ac-ETF$r8EYWAAL$SAA-NH2		1690.86	845.85	1691.87	846.44	564.63
SP196	204	Ac-LTF$r8AYWVAL$SAA-NH2		1644.93	824.08	1645.94	823.47	549.32
SP197	205	Ac-LTF$r8AHWAAL$SAA-NH2		1590.89	796.88	1591.9	796.45	531.3
SP198	206	Ac-LTF$r8AEWAAL$SAA-NH2		1582.88	791.9	1583.89	792.45	528.63
SP199	207	Ac-LTF$r8AEWAAL$SAA-NH2	iso2	1582.88	791.9	1583.89	792.45	528.63
SP200	208	Ac-LTF$r8ASWAAL$SAA-NH2		1540.87	770.74	1541.88	771.44	514.63
SP201	209	Ac-LTF$r8ASWAAL$SAA-NH2	iso2	1540.87	770.88	1541.88	771.44	514.63
SP202	210	Ac-LTF$r8AYWAAL$AAIa-NH2		1713.99	857.39	1715	858	572.34
SP203	211	Ac-LTF$r8AYWAAL$AAIa-NH2	iso2	1713.99	857.84	1715	858	572.34
SP204	212	Ac-LTF$r8AYWAAL$AHFA-NH2		1813.99	907.86	1815	908	605.67
SP205	213	Ac-LTF$r8EHWAQL$AHIa-NH2		1869.03	936.1	1870.04	935.52	624.02
SP206	214	Ac-LTF$r8EHWAQL$AHIa-NH2	iso2	1869.03	937.03	1870.04	935.52	624.02
SP207	215	Ac-LTF$r8AHWAQL$AHIa-NH2		1811.03	906.87	1812.04	906.52	604.68
SP208	216	Ac-LTF$r8EYWAQL$AHIa-NH2		1895.04	949.15	1896.05	948.53	632.69
SP209	217	Ac-LTF$r8AYWAQL$AAFa-NH2		1804.99	903.2	1806	903.5	602.67
SP210	218	Ac-LTF$r8AYWAQL$AAFa-NH2	iso2	1804.99	903.28	1806	903.5	602.67
SP211	219	Ac-LTF$r8AYWAQL$AAWa-NH2		1844	922.81	1845.01	923.01	615.67
SP212	220	Ac-LTF$r8AYWAQL$AAVa-NH2		1756.99	878.86	1758	879.5	586.67
SP213	221	Ac-LTF$r8AYWAQL$AAVa-NH2	iso2	1756.99	879.3	1758	879.5	586.67
SP214	222	Ac-LTF$r8AYWAQL$AALa-NH2		1771.01	886.26	1772.02	886.51	591.34
SP215	223	Ac-LTF$r8AYWAQL$AALa-NH2	iso2	1771.01	886.33	1772.02	886.51	591.34
SP216	224	Ac-LTF$r8EYWAQL$AAIa-NH2		1829.01	914.89	1830.02	915.51	610.68
SP217	225	Ac-LTF$r8EYWAQL$AAIa-NH2	iso2	1829.01	915.34	1830.02	915.51	610.68
SP218	226	Ac-LTF$r8EYWAQL$AAFa-NH2		1863	932.87	1864.01	932.51	622.01
SP219	227	Ac-LTF$r8EYWAQL$AAFa-NH2	iso2	1863	932.87	1864.01	932.51	622.01
SP220	228	Ac-LTF$r8EYWAQL$AAVa-NH2		1815	908.23	1816.01	908.51	606.01
SP221	229	Ac-LTF$r8EYWAQL$AAVa-NH2	iso2	1815	908.31	1816.01	908.51	606.01
SP222	230	Ac-LTF$r8EHWAQL$AAIa-NH2		1803.01	903.17	1804.02	902.51	602.01
SP223	231	Ac-LTF$r8EHWAQL$AAIa-NH2	iso2	1803.01	902.8	1804.02	902.51	602.01
SP224	232	Ac-LTF$r8EHWAQL$AAWa-NH2		1876	939.34	1877.01	939.01	626.34
SP225	233	Ac-LTF$r8EHWAQL$AAWa-NH2	iso2	1876	939.62	1877.01	939.01	626.34
SP226	234	Ac-LTF$r8EHWAQL$AALa-NH2		1803.01	902.8	1804.02	902.51	602.01
SP227	235	Ac-LTF$r8EHWAQL$AALa-NH2	iso2	1803.01	902.9	1804.02	902.51	602.01
SP228	236	Ac-ETF$r8EHWVQL$AALa-NH2		1847	924.82	1848.01	924.51	616.67
SP229	237	Ac-LTF$r8AYWAQL$AAAa-NH2		1728.96	865.89	1729.97	865.49	577.33
SP230	238	Ac-LTF$r8AYWAQL$AAAa-NH2	iso2	1728.96	865.89	1729.97	865.49	577.33
SP231	239	Ac-LTF$r8AYWAQL$AAAibA-NH2		1742.98	872.83	1743.99	872.5	582
SP232	240	Ac-LTF$r8AYWAQL$AAAibA-NH2	iso2	1742.98	872.92	1743.99	872.5	582
SP233	241	Ac-LTF$r8AYWAQL$AAAAa-NH2		1800	901.42	1801.01	901.01	601.01
SP234	242	Ac-LTF$r5AYWAQL$s8AAIa-NH2		1771.01	887.17	1772.02	886.51	591.34
SP235	243	Ac-LTF$r5AYWAQL$s8SAA-NH2		1673.92	838.33	1674.93	837.97	558.98
SP236	244	Ac-LTF$r8AYWAQCba$AANleA-		1783.01	892.64	1784.02	892.51	595.34
		NH2
SP237	245	Ac-ETF$r8AYWAQCba$AANleA-		1798.97	900.59	1799.98	900.49	600.66
		NH2
SP238	246	Ac-LTF$r8EYWAQCba$AANleA-		1841.01	922.05	1842.02	921.51	614.68
		NH2
SP239	247	Ac-LTF$r8AYWAQCba$AWNleA-		1898.05	950.46	1899.06	950.03	633.69
		NH2
SP240	248	Ac-ETF$r8AYWAQCba$AWNleA-		1914.01	958.11	1915.02	958.01	639.01
		NH2
SP241	249	Ac-LTF$r8EYWAQCba$AWNleA-		1956.06	950.62	1957.07	979.04	653.03
		NH2
SP242	250	Ac-LTF$r8EYWAQCba$SAFA-NH2		1890.99	946.55	1892	946.5	631.34
SP243	251	Ac-
		LTF34F2$r8EYWAQCba$SANleA-		1892.99	947.57	1894	947.5	632
		NH2
SP244	252	Ac-		1885	943.59	1886.01	943.51	629.34
		LTF$r8EF4coohWAQCba$SANleA-
		NH2
SP245	253	Ac-LTF$r8EYWSQCba$SANleA-NH2		1873	937.58	1874.01	937.51	625.34
SP246	254	Ac-LTF$r8EYWWQCba$SANleA-		1972.05	987.61	1973.06	987.03	658.36
		NH2
SP247	255	Ac-LTF$r8EYWAQCba$AAIa-NH2		1841.01	922.05	1842.02	921.51	614.68
SP248	256	Ac-LTF34F2$r8EYWAQCba$AAIa-		1876.99	939.99	1878	939.5	626.67
		NH2
SP249	257	Ac-LTF$r8EF4coohWAQCba$AAIa-		1869.01	935.64	1870.02	935.51	624.01
		NH2
SP250	258	Pam-ETF$r8EYWAQCba$SAA-NH2		1956.1	979.57	1957.11	979.06	653.04
SP251	259	Ac-LThF$r8EFWAQCba$SAA-NH2		1741.94	872.11	1742.95	871.98	581.65
SP252	260	Ac-LTA$r8EYWAQCba$SAA-NH2		1667.89	835.4	1668.9	834.95	556.97
SP253	261	Ac-LTF$r8EYAAQCba$SAA-NH2		1628.88	815.61	1629.89	815.45	543.97
SP254	262	Ac-LTF$r8EY2Na1AQCba$SAA-NH2		1754.93	879.04	1755.94	878.47	585.98
SP255	263	Ac-LTF$r8AYWAQCba$SAA-NH2		1685.92	844.71	1686.93	843.97	562.98
SP256	264	Ac-LTF$r8EYWAQCba$SAF-NH2		1819.96	911.41	1820.97	910.99	607.66
SP257	265	Ac-LTF$r8EYWAQCba$SAFa-NH2		1890.99	947.41	1892	946.5	631.34
SP258	266	Ac-LTF$r8AYWAQCba$SAF-NH2		1761.95	882.73	1762.96	881.98	588.32
SP259	267	Ac-LTF34F2$r8AYWAQCba$SAF-		1797.93	900.87	1798.94	899.97	600.32
		NH2
SP260	268	Ac-LTF$r8AF4coohWAQCba$SAF-		1789.94	896.43	1790.95	895.98	597.65
		NH2
SP261	269	Ac-LTF$r8EY6clWAQCba$SAF-NH2		1853.92	929.27	1854.93	927.97	618.98
SP262	270	Ac-LTF$r8AYWSQCba$SAF-NH2		1777.94	890.87	1778.95	889.98	593.65
SP263	271	Ac-LTF$r8AYWWQCba$SAF-NH2		1876.99	939.91	1878	939.5	626.67
SP264	272	Ac-LTF$r8AYWAQCba$AAIa-NH2		1783.01	893.19	1784.02	892.51	595.34
SP265	273	Ac-LTF34F2$r8AYWAQCba$AAIa-		1818.99	911.23	1820	910.5	607.34
		NH2
SP266	274	Ac-LTF$r8AY6clWAQCba$AAIa-		1816.97	909.84	1817.98	909.49	606.66
		NH2
SP267	275	Ac-LTF$r8AF4coohWAQCba$AAIa-		1811	906.88	1812.01	906.51	604.67
		NH2
SP268	276	Ac-LTF$r8EYWAQCba$AAFa-NH2		1875	938.6	1876.01	938.51	626.01
SP269	277	Ac-LTF$r8EYWAQCba$AAFa-NH2	iso2	1875	938.6	1876.01	938.51	626.01
SP270	278	Ac-ETF$r8AYWAQCba$AWNlea-		1914.01	958.42	1915.02	958.01	639.01
		NH2
SP271	279	Ac-LTF$r8EYWAQCba$AWNlea-		1956.06	979.42	1957.07	979.04	653.03
		NH2
SP272	280	Ac-ETF$r8EYWAQCba$AWNlea-		1972.01	987.06	1973.02	987.01	658.34
		NH2
SP273	281	Ac-ETF$r8EYWAQCba$AWNlea-	iso2	1972.01	987.06	1973.02	987.01	658.34
		NH2
SP274	282	Ac-LTF$r8AYWAQCba$SAFa-NH2		1832.99	917.89	1834	917.5	612
SP275	283	Ac-LTF$r8AYWAQCba$SAFa-NH2	iso2	1832.99	918.07	1834	917.5	612
SP276	284	Ac-ETF$r8AYWAQL$AWNlea-NH2		1902.01	952.22	1903.02	952.01	635.01
SP277	285	Ac-LTF$r8EYWAQL$AWNlea-NH2		1944.06	973.5	1945.07	973.04	649.03
SP278	286	Ac-ETF$r8EYWAQL$AWNlea-NH2		1960.01	981.46	1961.02	981.01	654.34
SP279	287	Dmaac-LTF$r8EYWAQhL$SAA-NH2		1788.98	896.06	1789.99	895.5	597.33
SP280	288	Hexac-LTF$r8EYWAQhL$SAA-NH2		1802	902.9	1803.01	902.01	601.67
SP281	289	Napac-LTF$r8EYWAQhL$SAA-NH2		1871.99	937.58	1873	937	625
SP282	290	Decac-LTF$r8EYWAQhL$SAA-NH2		1858.06	930.55	1859.07	930.04	620.36
SP283	291	Admac-LTF$r8EYWAQhL$SAA-NH2		1866.03	934.07	1867.04	934.02	623.02
SP284	292	Tmac-LTF$r8EYWAQhL$SAA-NH2		1787.99	895.41	1789	895	597
SP285	293	Pam-LTF$r8EYWAQhL$SAA-NH2		1942.16	972.08	1943.17	972.09	648.39
SP286	294	Ac-LTF$r8AYWAQCba$AANleA-	iso2	1783.01	892.64	1784.02	892.51	595.34
		NH2
SP287	295	Ac-LTF34F2$r8EYWAQCba$AAIa-	iso2	1876.99	939.62	1878	939.5	626.67
		NH2
SP288	296	Ac-LTF34F2$r8EYWAQCba$SAA-		1779.91	892.07	1780.92	890.96	594.31
		NH2
SP289	297	Ac-LTF34F2$r8EYWAQCba$SAA-	iso2	1779.91	891.61	1780.92	890.96	594.31
		NH2
SP290	298	Ac-LTF$r8EF4coohWAQCba$SAA-		1771.92	887.54	1772.93	886.97	591.65
		NH2
SP291	299	Ac-LTF$r8EF4coohWAQCba$SAA-	iso2	1771.92	887.63	1772.93	886.97	591.65
		NH2
SP292	300	Ac-LTF$r8EYWSQCba$SAA-NH2		1759.92	881.9	1760.93	880.97	587.65
SP293	301	Ac-LTF$r8EYWSQCba$SAA-NH2	iso2	1759.92	881.9	1760.93	880.97	587.65
SP294	302	Ac-LTF$r8EYWAQhL$SAA-NH2		1745.94	875.05	1746.95	873.98	582.99
SP295	303	Ac-LTF$r8AYWAQhL$SAF-NH2		1763.97	884.02	1764.98	882.99	589
SP296	304	Ac-LTF$r8AYWAQhL$SAF-NH2	iso2	1763.97	883.56	1764.98	882.99	589
SP297	305	Ac-LTF34F2$r8AYWAQhL$SAA-		1723.92	863.67	1724.93	862.97	575.65
		NH2
SP298	306	Ac-LTF34F2$r8AYWAQhL$SAA-	iso2	1723.92	864.04	1724.93	862.97	575.65
		NH2
SP299	307	Ac-LTF$r8AF4coohWAQhL$SAA-		1715.93	859.44	1716.94	858.97	572.98
		NH2
SP300	308	Ac-LTF$r8AF4coohWAQhL$SAA-	iso2	1715.93	859.6	1716.94	858.97	572.98
		NH2
SP301	309	Ac-LTF$r8AYWSQhL$SAA-NH2		1703.93	853.96	1704.94	852.97	568.98
SP302	310	Ac-LTF$r8AYWSQhL$SAA-NH2	iso2	1703.93	853.59	1704.94	852.97	568.98
SP303	311	Ac-LTF$r8EYWAQL$AANleA-NH2		1829.01	915.45	1830.02	915.51	610.68
SP304	312	Ac-LTF34F2$r8AYWAQL$AANleA-		1806.99	904.58	1808	904.5	603.34
		NH2
SP305	313	Ac-LTF$r8AF4coohWAQL$AANleA-		1799	901.6	1800.01	900.51	600.67
		NH2
SP306	314	Ac-LTF$r8AYWSQL$AANleA-NH2		1787	894.75	1788.01	894.51	596.67
SP307	315	Ac-LTF34F2$r8AYWAQhL$AANleA-		1821	911.79	1822.01	911.51	608.01
		NH2
SP308	316	Ac-LTF34F2$r8AYWAQhL$AANleA-	iso2	1821	912.61	1822.01	911.51	608.01
		NH2
SP309	317	Ac-		1813.02	907.95	1814.03	907.52	605.35
		LTF$r8AF4coohWAQhL$AANleA-
		NH2
SP310	318	Ac-	iso2	1813.02	908.54	1814.03	907.52	605.35
		LTF$r8AF4coohWAQhL$AANleA-
		NH2
SP311	319	Ac-LTF$r8AYWSQhL$AANleA-NH2		1801.02	901.84	1802.03	901.52	601.35
SP312	320	Ac-LTF$r8AYWSQhL$AANleA-NH2	iso2	1801.02	902.62	1802.03	901.52	601.35
SP313	321	Ac-LTF$r8AYWAQhL$AAAAa-NH2		1814.01	908.63	1815.02	908.01	605.68
SP314	322	Ac-LTF$r8AYWAQhL$AAAAa-NH2	iso2	1814.01	908.34	1815.02	908.01	605.68
SP315	323	Ac-LTF$r8AYWAQL$AAAAAa-NH2		1871.04	936.94	1872.05	936.53	624.69
SP316	324	Ac-LTF$r8AYWAQL$AAAAAAa-	iso2	1942.07	972.5	1943.08	972.04	648.37
		NH2
SP317	325	Ac-LTF$r8AYWAQL$AAAAAAa-	iso1	1942.07	972.5	1943.08	972.04	648.37
		NH2
SP318	326	Ac-LTF$r8EYWAQhL$AANleA-NH2		1843.03	922.54	1844.04	922.52	615.35
SP319	327	Ac-AATF$r8AYWAQL$AANleA-		1800	901.39	1801.01	901.01	601.01
		NH2
SP320	328	Ac-LTF$r8AYWAQL$AANleAA-NH2		1842.04	922.45	1843.05	922.03	615.02
SP321	329	Ac-ALTF$r8AYWAQL$AANleAA-		1913.08	957.94	1914.09	957.55	638.7
		NH2
SP322	330	Ac-LTF$r8AYWAQCba$AANleAA-		1854.04	928.43	1855.05	928.03	619.02
		NH2
SP323	331	Ac-LTF$r8AYWAQhL$AANleAA-		1856.06	929.4	1857.07	929.04	619.69
		NH2
SP324	332	Ac-LTF$r8EYWAQCba$SAAA-NH2		1814.96	909.37	1815.97	908.49	605.99
SP325	333	Ac-LTF$r8EYWAQCba$SAAA-NH2	iso2	1814.96	909.37	1815.97	908.49	605.99
SP326	334	Ac-LTF$r8EYWAQCba$SAAAA-NH2		1886	944.61	1887.01	944.01	629.67
SP327	335	Ac-LTF$r8EYWAQCba$SAAAA-NH2	iso2	1886	944.61	1887.01	944.01	629.67
SP328	336	Ac-ALTF$r8EYWAQCba$SAA-NH2		1814.96	909.09	1815.97	908.49	605.99
SP329	337	Ac-ALTF$r8EYWAQCba$SAAA-NH2		1886	944.61	1887.01	944.01	629.67
SP330	338	Ac-ALTF$r8EYWAQCba$SAA-NH2	iso2	1814.96	909.09	1815.97	908.49	605.99
SP331	339	Ac-LTF$r8EYWAQL$AAAAAa-NH2	iso2	1929.04	966.08	1930.05	965.53	644.02
SP332	340	Ac-LTF$r8EY6clWAQCba$SAA-NH2		1777.89	890.78	1778.9	889.95	593.64
SP333	341	Ac-		1918.96	961.27	1919.97	960.49	640.66
		LTF$r8EF4cooh6clWAQCba$SANleA-
		NH2
SP334	342	Ac-	iso2	1918.96	961.27	1919.97	960.49	640.66
		LTF$r8EF4cooh6clWAQCba$SANleA-
		NH2
SP335	343	Ac-		1902.97	953.03	1903.98	952.49	635.33
		LTF$r8EF4cooh6clWAQCba$AAIa-
		NH2
SP336	344	Ac-	iso2	1902.97	953.13	1903.98	952.49	635.33
		LTF$r8EF4cooh6clWAQCba$AAIa-
		NH2
SP337	345	Ac-LTF$r8AY6clWAQL$AAAAAa-		1905	954.61	1906.01	953.51	636.01
		NH2
SP338	346	Ac-LTF$r8AY6clWAQL$AAAAAa-	iso2	1905	954.9	1906.01	953.51	636.01
		NH2
SP339	347	Ac-F$r8AY6clWEAL$AAAAAAa-		1762.89	883.01	1763.9	882.45	588.64
		NH2
SP340	348	Ac-ETF$r8EYWAQL$AAAAAa-NH2		1945	974.31	1946.01	973.51	649.34
SP341	349	Ac-ETF$r8EYWAQL$AAAAAa-NH2	iso2	1945	974.49	1946.01	973.51	649.34
SP342	350	Ac-LTF$r8EYWAQL$AAAAAAa-		2000.08	1001.6	2001.09	1001.05	667.7
		NH2
SP343	351	Ac-LTF$r8EYWAQL$AAAAAAa-	iso2	2000.08	1001.6	2001.09	1001.05	667.7
		NH2
SP344	352	Ac-LTF$r8AYWAQL$AANleAAa-		1913.08	958.58	1914.09	957.55	638.7
		NH2
SP345	353	Ac-LTF$r8AYWAQL$AANleAAa-	iso2	1913.08	958.58	1914.09	957.55	638.7
		NH2
SP346	354	Ac-LTF$r8EYWAQCba$AAAAAa-		1941.04	972.55	1942.05	971.53	648.02
		NH2
SP347	355	Ac-LTF$r8EYWAQCba$AAAAAa-	iso2	1941.04	972.55	1942.05	971.53	648.02
		NH2
SP348	356	Ac-		1969.04	986.33	1970.05	985.53	657.35
		LTF$r8EF4coohWAQCba$AAAAAa-
		NH2
SP349	357	Ac-	iso2	1969.04	986.06	1970.05	985.53	657.35
		LTF$r8EF4coohWAQCba$AAAAAa-
		NH2
SP350	358	Ac-LTF$r8EYWSQCba$AAAAAa-		1957.04	980.04	1958.05	979.53	653.35
		NH2
SP351	359	Ac-LTF$r8EYWSQCba$AAAAAa-	iso2	1957.04	980.04	1958.05	979.53	653.35
		NH2
SP352	360	Ac-LTF$r8EYWAQCbaSSAAa-NH2		1814.96	909	1815.97	908.49	605.99
SP353	361	Ac-LTF$r8EYWAQCba$SAAa-NH2	iso2	1814.96	909	1815.97	908.49	605.99
SP354	362	Ac-ALTF$r8EYWAQCbaSSAAa-NH2		1886	944.52	1887.01	944.01	629.67
SP355	363	Ac-ALTF$r8EYWAQCba$SAAa-NH2	iso2	1886	944.98	1887.01	944.01	629.67
SP356	364	Ac-ALTF$r8EYWAQCbaSSAAAa-
		NH2		1957.04	980.04	1958.05	979.53	653.35
SP357	365	Ac-ALTF$r8EYWAQCbaSSAAAa-
		NH2	iso2	1957.04	980.04	1958.05	979.53	653.35
SP358	366	Ac-AALTF$r8EYWAQCbaSSAAAa-
		NH2		2028.07	1016.1	2029.08	1015.04	677.03
SP359	367	Ac-AALTF$r8EYWAQCbaSSAAAa-
		NH2	iso2	2028.07	1015.57	2029.08	1015.04	677.03
SP360	368	Ac-RTF$r8EYWAQCba$SAA-NH2		1786.94	895.03	1787.95	894.48	596.65
SP361	369	Ac-LRF$r8EYWAQCba$SAA-NH2		1798.98	901.51	1799.99	900.5	600.67
SP362	370	Ac-LTF$r8EYWRQCba$SAA-NH2		1828.99	916.4	1830	915.5	610.67
SP363	371	Ac-LTF$r8EYWARCba$SAA-NH2		1771.97	887.63	1772.98	886.99	591.66
SP364	372	Ac-LTF$r8EYWAQCba$RAA-NH2		1812.99	908.08	1814	907.5	605.34
SP365	373	Ac-LTF$r8EYWAQCba$SRA-NH2		1828.99	916.12	1830	915.5	610.67
SP366	374	Ac-LTF$r8EYWAQCba$SAR-NH2		1828.99	916.12	1830	915.5	610.67
SP367	375	5-FAM-BaLTF$r8EYWAQCbaSSAA-		2131	1067.09	2132.01	1066.51	711.34
		NH2
SP368	376	5-FAM-		2158.08	1080.6	2159.09	1080.05	720.37
		BaLTF$r8AYWAQL$AANleA-NH2
SP369	377	Ac-LAF$r8EYWAQL$AANleA-NH2		1799	901.05	1800.01	900.51	600.67
SP370	378	Ac-ATF$r8EYWAQL$AANleA-NH2		1786.97	895.03	1787.98	894.49	596.66
SP371	379	Ac-AAF$r8EYWAQL$AANleA-NH2		1756.96	880.05	1757.97	879.49	586.66
SP372	380	Ac-AAAF$r8EYWAQL$AANleA-		1827.99	915.57	1829	915	610.34
		NH2
SP373	381	Ac-AAAAF$r8EYWAQL$AANleA-		1899.03	951.09	1900.04	950.52	634.02
		NH2
SP374	382	Ac-AATF$r8EYWAQL$AANleA-NH2		1858	930.92	1859.01	930.01	620.34
SP375	383	Ac-AALTF$r8EYWAQL$AANleA-		1971.09	987.17	1972.1	986.55	658.04
		NH2
SP376	384	Ac-AAALTF$r8EYWAQL$AANleA-		2042.12	1023.15	2043.13	1022.07	681.71
		NH2
SP377	385	Ac-LTF$r8EYWAQL$AANleAA-NH2		1900.05	952.02	1901.06	951.03	634.36
SP378	386	Ac-ALTF$r8EYWAQL$AANleAA-		1971.09	987.63	1972.1	986.55	658.04
		NH2
SP379	387	Ac-AALTF$r8EYWAQL$AANleAA-		2042.12	1022.69	2043.13	1022.07	681.71
		NH2
SP380	388	Ac-LTF$r8EYWAQCba$AANleAA-		1912.05	958.03	1913.06	957.03	638.36
		NH2
SP381	389	Ac-LTF$r8EYWAQhL$AANleAA-		1914.07	958.68	1915.08	958.04	639.03
		NH2
SP382	390	Ac-ALTF$r8EYWAQhL$AANleAA-		1985.1	994.1	1986.11	993.56	662.71
		NH2
SP383	391	Ac-LTF$r8ANmYWAQL$AANleA-		1785.02	894.11	1786.03	893.52	596.01
		NH2
SP384	392	Ac-LTF$r8ANmYWAQL$AANleA-	iso2	1785.02	894.11	1786.03	893.52	596.01
		NH2
SP385	393	Ac-LTF$r8AYNmWAQL$AANleA-		1785.02	894.11	1786.03	893.52	596.01
		NH2
SP386	394	Ac-LTF$r8AYNmWAQL$AANleA-	iso2	1785.02	894.11	1786.03	893.52	596.01
		NH2
SP387	395	Ac-LTF$r8AYAmwAQL$AANleA-		1785.02	894.01	1786.03	893.52	596.01
		NH2
SP388	396	Ac-LTF$r8AYAmwAQL$AANleA-	iso2	1785.02	894.01	1786.03	893.52	596.01
		NH2
SP389	397	Ac-LTF$r8AYWAibQL$AANleA-NH2		1785.02	894.01	1786.03	893.52	596.01
SP390	398	Ac-LTF$r8AYWAibQL$AANleA-NH2	iso2	1785.02	894.01	1786.03	893.52	596.01
SP391	399	Ac-LTF$r8AYWAQL$AAibNleA-NH2		1785.02	894.38	1786.03	893.52	596.01
SP392	400	Ac-LTF$r8AYWAQL$AAibNleA-NH2	iso2	1785.02	894.38	1786.03	893.52	596.01
SP393	401	Ac-LTF$r8AYWAQL$AaNleA-NH2		1771.01	887.54	1772.02	886.51	591.34
SP394	402	Ac-LTF$r8AYWAQL$AaNleA-NH2	iso2	1771.01	887.54	1772.02	886.51	591.34
SP395	403	Ac-LTF$r8AYWAQL$ASarNleA-NH2		1771.01	887.35	1772.02	886.51	591.34
SP396	404	Ac-LTF$r8AYWAQL$ASarNleA-NH2	iso2	1771.01	887.35	1772.02	886.51	591.34
SP397	405	Ac-LTF$r8AYWAQL$AANleAib-NH2		1785.02	894.75	1786.03	893.52	596.01
SP398	406	Ac-LTF$r8AYWAQL$AANleAib-NH2	iso2	1785.02	894.75	1786.03	893.52	596.01
SP399	407	Ac-LTF$r8AYWAQL$AANleNmA-		1785.02	894.6	1786.03	893.52	596.01
		NH2
SP400	408	Ac-LTF$r8AYWAQL$AANleNmA-	iso2	1785.02	894.6	1786.03	893.52	596.01
		NH2
SP401	409	Ac-LTF$r8AYWAQL$AANleSar-NH2		1771.01	886.98	1772.02	886.51	591.34
SP402	410	Ac-LTF$r8AYWAQL$AANleSar-NH2	iso2	1771.01	886.98	1772.02	886.51	591.34
SP403	411	Ac-LTF$r8AYWAQL$AANleAAib-			1856.06	1857.07	929.04	619.69
		NH2
SP404	412	Ac-LTF$r8AYWAQL$AANleAAib-	iso2		1856.06	1857.07	929.04	619.69
		NH2
SP405	413	Ac-LTF$r8AYWAQL$AANleANmA-		1856.06	930.37	1857.07	929.04	619.69
		NH2
SP406	414	Ac-LTF$r8AYWAQL$AANleANmA-	iso2	1856.06	930.37	1857.07	929.04	619.69
		NH2
SP407	415	Ac-LTF$r8AYWAQL$AANleAa-NH2		1842.04	922.69	1843.05	922.03	615.02
SP408	416	Ac-LTF$r8AYWAQL$AANleAa-NH2	iso2	1842.04	922.69	1843.05	922.03	615.02
SP409	417	Ac-LTF$r8AYWAQL$AANleASar-		1842.04	922.6	1843.05	922.03	615.02
		NH2
SP410	418	Ac-LTF$r8AYWAQL$AANleASar-	iso2	1842.04	922.6	1843.05	922.03	615.02
		NH2
SP411	419	Ac-LTF$/r8AYWAQLVAANleA-NH2		1799.04	901.14	1800.05	900.53	600.69
SP412	420	Ac-LTFAibAYWAQLAibAANleA-		1648.9	826.02	1649.91	825.46	550.64
		NH2
SP413	421	Ac-LTF$r8Cou4YWAQL$AANleA-		1975.05	989.11	1976.06	988.53	659.36
		NH2
SP414	422	Ac-LTF$r8Cou4YWAQL$AANleA-	iso2	1975.05	989.11	1976.06	988.53	659.36
		NH2
SP415	423	Ac-LTF$r8AYWCou4QL$AANleA-		1975.05	989.11	1976.06	988.53	659.36
		NH2
SP416	424	Ac-LTF$r8AYWAQL$Cou4ANleA-		1975.05	989.57	1976.06	988.53	659.36
		NH2
SP417	425	Ac-LTF$r8AYWAQL$Cou4ANleA-	iso2	1975.05	989.57	1976.06	988.53	659.36
		NH2
SP418	426	Ac-LTF$r8AYWAQL$ACou4NleA-		1975.05	989.57	1976.06	988.53	659.36
		NH2
SP419	427	Ac-LTF$r8AYWAQL$ACou4NleA-	iso2	1975.05	989.57	1976.06	988.53	659.36
		NH2
SP420	428	Ac-LTF$r8AYWAQL$AANleA-OH		1771.99	887.63	1773	887	591.67
SP421	429	Ac-LTF$r8AYWAQL$AANleA-OH	iso2	1771.99	887.63	1773	887	591.67
SP422	430	Ac-LTF$r8AYWAQL$AANleA-		1813.05	908.08	1814.06	907.53	605.36
		NHnPr
SP423	431	Ac-LTF$r8AYWAQL$AANleA-	iso2	1813.05	908.08	1814.06	907.53	605.36
		NHnPr
SP424	432	Ac-LTF$r8AYWAQL$AANleA-		1855.1	929.17	1856.11	928.56	619.37
		NHnBu33Me
SP425	433	Ac-LTF$r8AYWAQL$AANleA-	iso2	1855.1	929.17	1856.11	928.56	619.37
		NHnBu33Me
SP426	434	Ac-LTF$r8AYWAQL$AANleA-		1855.1	929.17	1856.11	928.56	619.37
		NI-1Hex
SP427	435	Ac-LTF$r8AYWAQL$AANleA-	iso2	1855.1	929.17	1856.11	928.56	619.37
		NI-1Hex
SP428	436	Ac-LTA$r8AYWAQL$AANleA-NH2		1694.98	849.33	1695.99	848.5	566
SP429	437	Ac-LThL$r8AYWAQL$AANleA-NH2		1751.04	877.09	1752.05	876.53	584.69
SP430	438	Ac-LTF$r8AYAAQL$AANleA-NH2		1655.97	829.54	1656.98	828.99	553
SP431	439	Ac-LTF$r8AY2Na1AQL$AANleA-		1782.01	892.63	1783.02	892.01	595.01
		NH2
SP432	440	Ac-LTF$r8EYWCou4QCba$SAA-NH2		1947.97	975.8	1948.98	974.99	650.33
SP433	441	Ac-LTF$r8EYWCou7QCba$SAA-NH2		16.03	974.9	17.04	9.02	6.35
SP434	442	Ac-LTF%r8EYWAQCba%SAA-NH2		1745.94	874.8	1746.95	873.98	582.99
SP435	443	Dmaac-LTF$r8EYWAQCba$SAA-		1786.97	894.8	1787.98	894.49	596.66
		NH2
SP436	444	Dmaac-LTF$r8AYWAQL$AAAAAa-		1914.08	958.2	1915.09	958.05	639.03
		NH2	iso2
SP437	445	Dmaac-LTF$r8AYWAQL$AAAAAa-		1914.08	958.2	1915.09	958.05	639.03
		NH2
SP438	446	Dmaac-LTF$r8EYWAQL$AAAAAa-		1972.08	987.3	1973.09	987.05	658.37
		NH2
SP439	447	Dmaac-LTF$r8EYWAQL$AAAAAa-	iso2	1972.08	987.3	1973.09	987.05	658.37
		NH2
SP440	448	Dmaac-		1912.05	957.4	1913.06	957.03	638.36
		LTF$r8EF4coohWAQCba$AAIa-NH2
SP441	449	Dmaac-LTF$r8EF4coohWAQCba$AAIa-	iso2	1912.05	957.4	1913.06	957.03	638.36
		NH2
SP442	450	Dmaac-LTF$r8AYWAQL$AANleA-		1814.05	908.3	1815.06	908.03	605.69
		NH2
SP443	451	Dmaac-LTF$r8AYWAQL$AANleA-	iso2	1814.05	908.3	1815.06	908.03	605.69
		NH2
SP444	452	Ac-LTF%r8AYWAQL%AANleA-NH2		1773.02	888.37	1774.03	887.52	592.01
SP445	453	Ac-LTF%r8EYWAQL%AAAAAa-		1931.06	966.4	1932.07	966.54	644.69
		NH2
SP446	454	Cou6BaLTF$r8EYWAQhL$SAA-NH2		2018.05	1009.9	2019.06	1010.03	673.69
SP447	455	Cou8BaLTF$r8EYWAQhL$SAA-NH2		1962.96	982.34	1963.97	982.49	655.32
SP448	456	Ac-LTF4M8EYWAQL$AAAAAa-		2054.93	1028.68	2055.94	1028.47	685.98
		NH2
SP449	457	Ac-LTF$r8EYWAQL$AAAAAa-NH2		1929.04	966.17	1930.05	965.53	644.02
SP550	458	Ac-LTF$r8EYWAQL$AAAAAa-OH		1930.02	966.54	1931.03	966.02	644.35
SP551	459	Ac-LTF$r8EYWAQL$AAAAAa-OH	iso2	1930.02	965.89	1931.03	966.02	644.35
SP552	460	Ac-LTF$r8EYWAEL$AAAAAa-NH2		1930.02	966.82	1931.03	966.02	644.35
SP553	461	Ac-LTF$r8EYWAEL$AAAAAa-NH2	iso2	1930.02	966.91	1931.03	966.02	644.35
SP554	462	Ac-LTF$r8EYWAEL$AAAAAa-OH		1931.01	967.28	1932.02	966.51	644.68
SP555	463	Ac-LTF$r8EY6clWAQL$AAAAAa-		1963	983.28	1964.01	982.51	655.34
		NH2
SP556	464	Ac-		1957.05	980.04	1958.06	979.53	653.36
		LTF$r8EF4bOH2WAQL$AAAAAa-
		NH2
SP557	465	Ac-AAALTF$r8EYWAQL$AAAAAa-		2142.15	1072.83	2143.16	1072.08	715.06
		NH2
SP558	466	Ac-LTF34F2$r8EYWAQL$AAAAAa-		1965.02	984.3	1966.03	983.52	656.01
		NH2
SP559	467	Ac-RTF$r8EYWAQL$AAAAAa-NH2		1972.06	987.81	1973.07	987.04	658.36
SP560	468	Ac-LTA$r8EYWAQL$AAAAAa-NH2		1853.01	928.33	1854.02	927.51	618.68
SP561	469	Ac-LTF$r8EYWAibQL$AAAAAa-		1943.06	973.48	1944.07	972.54	648.69
		NH2
SP562	470	Ac-LTF$r8EYWAQL$AAibAAAa-		1943.06	973.11	1944.07	972.54	648.69
		NH2
SP563	471	Ac-LTF$r8EYWAQL$AAAibAAa-		1943.06	973.48	1944.07	972.54	648.69
		NH2
SP564	472	Ac-LTF$r8EYWAQL$AAAAibAa-		1943.06	973.48	1944.07	972.54	648.69
		NH2
SP565	473	Ac-LTF$r8EYWAQL$AAAAAiba-		1943.06	973.38	1944.07	972.54	648.69
		NH2
SP566	474	Ac-LTF$r8EYWAQL$AAAAAiba-	iso2	1943.0	6 973.38	1944.07	972.54	648.69
		NH2
SP567	475	Ac-LTF$r8EYWAQL$AAAAAAib-		1943.06	973.01	1944.07	972.54	648.69
		NH2
SP568	476	Ac-LTF$r8EYWAQL$AaAAAa-NH2		1929.04	966.54	1930.05	965.53	644.02
SP569	477	Ac-LTF$r8EYWAQL$AAaAAa-NH2		1929.04	966.35	1930.05	965.53	644.02
SP570	478	Ac-LTF$r8EYWAQL$AAAaAa-NH2		1929.04	966.54	1930.05	965.53	644.02
SP571	479	Ac-LTF$r8EYWAQL$AAAaAa-NH2	iso2	1929.04	966.35	1930.05	965.53	644.02
SP572	480	Ac-LTF$r8EYWAQL$AAAAaa-NH2		1929.04	966.35	1930.05	965.53	644.02
SP573	481	Ac-LTF$r8EYWAQL$AAAAAA-NH2		1929.04	966.35	1930.05	965.53	644.02
SP574	482	Ac-LTF$r8EYWAQL$ASarAAAa-		1929.04	966.54	1930.05	965.53	644.02
		NH2
SP575	483	Ac-LTF$r8EYWAQL$AASarAAa-		1929.04	966.35	1930.05	965.53	644.02
		NH2
SP576	484	Ac-LTF$r8EYWAQL$AAASarAa-		1929.04	966.35	1930.05	965.53	644.02
		NH2
SP577	485	Ac-LTF$r8EYWAQL$AAAASara-		1929.04	966.35	1930.05	965.53	644.02
		NH2
SP578	486	Ac-LTF$r8EYWAQL$AAAAASar-		1929.04	966.08	1930.05	965.53	644.02
		NH2
SP579	487	Ac-7LTF$r8EYWAQL$AAAAAa-		1918.07	951.99	1919.08	960.04	640.37
		NH2
SP581	488	Ac-TF$r8EYWAQL$AAAAAa-NH2		1815.96	929.85	1816.97	908.99	606.33
SP582	489	Ac-F$r8EYWAQL$AAAAAa-NH2		1714.91	930.92	1715.92	858.46	572.64
SP583	490	Ac-LVF$r8EYWAQL$AAAAAa-NH2		1927.06	895.12	1928.07	964.54	643.36
SP584	491	Ac-AAF$r8EYWAQL$AAAAAa-NH2		1856.98	859.51	1857.99	929.5	620
SP585	492	Ac-LTF$r8EYWAQL$AAAAa-NH2		1858	824.08	1859.01	930.01	620.34
SP586	493	Ac-LTF$r8EYWAQL$AAAa-NH2		1786.97	788.56	1787.98	894.49	596.66
SP587	494	Ac-LTF$r8EYWAQL$AAa-NH2		1715.93	1138.57	1716.94	858.97	572.98
SP588	495	Ac-LTF$r8EYWAQL$Aa-NH2		1644.89	1144.98	1645.9	823.45	549.3
SP589	496	Ac-LTF$r8EYWAQL$a-NH2		1573.85	1113.71	1574.86	787.93	525.62
SP590	497	Ac-LTF$r8EYWAQL$AAA-OH		1716.91	859.55	1717.92	859.46	573.31
SP591	498	Ac-LTF$r8EYWAQL$A-OH		1574.84	975.14	1575.85	788.43	525.95
SP592	499	Ac-LTF$r8EYWAQL$AAA-NH2		1715.93	904.75	1716.94	858.97	572.98
SP593	500	Ac-LTF$r8EYWAQCba$SAA-OH		1744.91	802.49	1745.92	873.46	582.64
SP594	501	Ac-LTF$r8EYWAQCba$S-OH		1602.83	913.53	1603.84	802.42	535.28
SP595	502	Ac-LTF$r8EYWAQCba$S-NH2		1601.85	979.58	1602.86	801.93	534.96
SP596	503	4-FBzl-LTF$r8EYWAQL$AAAAAa-		2009.05	970.52	2010.06	1005.53	670.69
		NH2
SP597	504	4-FBzl-LTF$r8EYWAQCba$SAA-		1823.93	965.8	1824.94	912.97	608.98
		NH2
SP598	505	Ac-LTF$r8RYWAQL$AAAAAa-NH2		1956.1	988.28	1957.11	979.06	653.04
SP599	506	Ac-LTF$r8HYWAQL$AAAAAa-NH2		1937.06	1003.54	1938.07	969.54	646.69
SP600	507	Ac-LTF$r8QYWAQL$AAAAAa-NH2		1928.06	993.92	1929.07	965.04	643.69
SP601	508	Ac-LTF$r8CitYWAQL$AAAAAa-		1957.08	987	1958.09	979.55	653.37
		NH2
SP602	509	Ac-LTF$r8GlaYWAQL$AAAAAa-		1973.03	983	1974.04	987.52	658.68
		NH2
SP603	510	Ac-LTF$r8F4gYWAQL$AAAAAa-		2004.1	937.86	2005.11	1003.06	669.04
		NH2
SP604	511	Ac-LTF$r82mRYWAQL$AAAAAa-		1984.13	958.58	1985.14	993.07	662.38
		NH2
SP605	512	Ac-LTF$r8ipKYWAQL$AAAAAa-		1970.14	944.52	1971.15	986.08	657.72
		NH2
SP606	513	Ac-LTF$r8F4NH2YWAQL$AAAAAa-		1962.08	946	1963.09	982.05	655.03
		NH2
SP607	514	Ac-LTF$r8EYWAAL$AAAAAa-NH2		1872.02	959.32	1873.03	937.02	625.01
SP608	515	Ac-LTF$r8EYWALL$AAAAAa-NH2		1914.07	980.88	1915.08	958.04	639.03
SP609	516	Ac-LTF$r8EYWAAibL$AAAAAa-		1886.03	970.61	1887.04	944.02	629.68
		NH2
SP610	517	Ac-LTF$r8EYWASL$AAAAAa-NH2		1888.01	980.51	1889.02	945.01	630.34
SP611	518	Ac-LTF$r8EYWANL$AAAAAa-NH2		1915.02	1006.41	1916.03	958.52	639.35
SP612	519	Ac-LTF$r8EYWACAL$AAAAAa-		1958.07		1959.08	980.04	653.7
		NH2
SP613	520	Ac-LTF$r8EYWAHL$AAAAAa-NH2		1938.04	966.24	1939.05	970.03	647.02
SP614	521	Ac-LTF$r8EYWARL$AAAAAa-NH2		1957.08		1958.09	979.55	653.37
SP615	522	Ac-LTF$r8EpYWAQL$AAAAAa-		2009.01		2010.02	1005.51	670.68
		NH2
SP616	523	Cbm-LTF$r8EYWAQCba$SAA-NH2		1590.85		1591.86	796.43	531.29
SP617	524	Cbm-LTF$r8EYWAQL$AAAAAa-		1930.04		1931.05	966.03	644.35
		NH2
SP618	525	Ac-LTF$r8EYWAQL$SAAAAa-NH2		1945.04	1005.11	1946.05	973.53	649.35
SP619	526	Ac-LTF$r8EYWAQL$AAAASa-NH2		1945.04	986.52	1946.05	973.53	649.35
SP620	527	Ac-LTF$r8EYWAQL$SAAASa-NH2		1961.03	993.27	1962.04	981.52	654.68
SP621	528	Ac-LTF$r8EYWAQTba$AAAAAa-		1943.06	983.1	1944.07	972.54	648.69
		NH2
SP622	529	Ac-LTF$r8EYWAQAdm$AAAAAa-		2007.09	990.31	2008.1	1004.55	670.04
		NH2
SP623	530	Ac-LTF$r8EYWAQCha$AAAAAa-		1969.07	987.17	1970.08	985.54	657.36
		NH2
SP624	531	Ac-LTF$r8EYWAQhCha$AAAAAa-		1983.09	1026.11	1984.1	992.55	662.04
		NH2
SP625	532	Ac-LTF$r8EYWAQF$AAAAAa-NH2		1963.02	957.01	1964.03	982.52	655.35
SP626	533	Ac-LTF$r8EYWAQhF$AAAAAa-NH2		1977.04	1087.81	1978.05	989.53	660.02
SP627	534	Ac-LTF$r8EYWAQL$AANleAAa-		1971.09	933.45	1972.1	986.55	658.04
		NH2
SP628	535	Ac-LTF$r8EYWAQAdm$AANleAAa-		2049.13	1017.97	2050.14	1025.57	684.05
		NH2
SP629	536	4-FBz-		2080.08		2081.09	1041.05	694.37
		BaLTF$r8EYWAQL$AAAAAa-NH2
SP630	537	4-FBz-BaLTF$r8EYWAQCba$SAA-		1894.97		1895.98	948.49	632.66
		NH2
SP631	538	Ac-LTF$r5EYWAQL$s8AAAAAa-		1929.04	1072.68	1930.05	965.53	644.02
		NH2
SP632	539	Ac-LTF$r5EYWAQCbaSs8SAA-NH2		1743.92	1107.79	1744.93	872.97	582.31
SP633	540	Ac-LTF$r8EYWAQL$AAhhLAAa-		1999.12		2000.13	1000.57	667.38
		NH2
SP634	541	Ac-LTF$r8EYWAQL$AAAAAAAa-		2071.11		2072.12	1036.56	691.38
		NH2
SP635	542	Ac-LTF$r8EYWAQL$AAAAAAAAa-		2142.15	778.1	2143.16	1072.08	715.06
		NH2
SP636	543	Ac-		2213.19	870.53	2214.2	1107.6	738.74
		LTF$r8EYWAQL$AAAAAAAAAa-
		NH2
SP637	544	Ac-LTA$r8EYAAQCba$SAA-NH2		1552.85		1553.86	777.43	518.62
SP638	545	Ac-LTA$r8EYAAQL$AAAAAa-NH2		1737.97	779.45	1738.98	869.99	580.33
SP639	546	Ac-LTF$r8EPmpWAQL$AAAAAa-		2007.03	779.54	2008.04	1004.52	670.02
		NH2
SP640	547	Ac-LTF$r8EPmpWAQCba$SAA-NH2		1821.91	838.04	1822.92	911.96	608.31
SP641	548	Ac-ATF$r8HYWAQL$S-NH2		1555.82	867.83	1556.83	778.92	519.61
SP642	549	Ac-LTF$r8HAWAQL$S-NH2		1505.84	877.91	1506.85	753.93	502.95
SP643	550	Ac-LTF$r8HYWAQA$S-NH2		1555.82	852.52	1556.83	778.92	519.61
SP644	551	Ac-LTF$r8EYWAQCba$SA-NH2		1672.89	887.18	1673.9	837.45	558.64
SP645	552	Ac-LTF$r8EYWAQL$SAA-NH2		1731.92	873.32	1732.93	866.97	578.31
SP646	553	Ac-LTF$r8HYWAQCba$SAA-NH2		1751.94	873.05	1752.95	876.98	584.99
SP647	554	Ac-LTF$r8SYWAQCba$SAA-NH2		1701.91	844.88	1702.92	851.96	568.31
SP648	555	Ac-LTF$r8RYWAQCba$SAA-NH2		1770.98	865.58	1771.99	886.5	591.33
SP649	556	Ac-LTF$r8KYWAQCba$SAA-NH2		1742.98	936.57	1743.99	872.5	582
SP650	557	Ac-LTF$r8QYWAQCba$SAA-NH2		1742.94	930.93	1743.95	872.48	581.99
SP651	558	Ac-LTF$r8EYWAACba$SAA-NH2		1686.9	1032.45	1687.91	844.46	563.31
SP652	559	Ac-LTF$r8EYWAQCba$AAA-NH2		1727.93	895.46	1728.94	864.97	576.98
SP653	560	Ac-LTF$r8EYWAQL$AAAAA-OH		1858.99	824.54	1860	930.5	620.67
SP654	561	Ac-LTF$r8EYWAQL$AAAA-OH		1787.95	894.48	1788.96	894.98	596.99
SP655	562	Ac-LTF$r8EYWAQL$AA-OH		1645.88	856	1646.89	823.95	549.63
SP656	563	Ac-
		LTF$r8AF4bOH2WAQL$AAAAAa-
		NH2
SP657	564	Ac-
		LTF$r8AF4bOH2WAAL$AAAAAa-
		NH2
SP658	565	Ac-LTF$r8EF4bOH2WAQCba$SAA-
		NH2
SP659	566	Ac-LTF$r8ApYWAQL$AAAAAa-
		NH2
SP660	567	Ac-LTF$r8ApYWAAL$AAAAAa-
		NH2
SP661	568	Ac-LTF$r8EpYWAQCba$SAA-NH2
SP662	569	Ac-		1974.06	934.44
		LTF$rda6AYWAQL$da5AAAAAa-
		NH2
SP663	570	Ac-LTF$rda6EYWAQCba$da5SAA-		1846.95	870.52		869.94
		NH2
SP664	571	Ac-LTF$rda6EYWAQL$da5AAAAAa-
		NH2
SP665	572	Ac-LTF$ra9EYWAQL$a6AAAAAa-			936.57		935.51
		NH2
SP666	573	Ac-LTF$ra9EYWAQL$a6AAAAAa-
		NH2
SP667	574	Ac-LTF$ra9EYWAQCba$a6SAA-NH2
SP668	575	Ac-LTA$ra9EYWAQCbaSa6SAA-
		NH2
SP669	576	5-FAM-
		BaLTF$ra9EYWAQCba$a6SAA-NH2
SP670	577	5-FAM-		2316.11
		BaLTF$r8EYWAQL$AAAAAa-NH2
SP671	578	5-FAM-		2344.15
		BaLTF$/r8EYWAQLVAAAAAa-NH2
SP672	579	5-FAM-		2240.08
		BaLTA$r8EYWAQL$AAAAAa-NH2
SP673	580	5-FAM-		2258.11
		BaLTF$r8AYWAQL$AAAAAa-NH2
SP674	581	5-FAM-		2274.07
		BaATF$r8EYWAQL$AAAAAa-NH2
SP675	582	5-FAM-		2286.1
		BaLAF$r8EYWAQL$AAAAAa-NH2
SP676	583	5-FAM-		2224.09
		BaLTF$r8EAWAQL$AAAAAa-NH2
SP677	584	5-FAM-		2201.07
		BaLTF$r8EYAAQL$AAAAAa-NH2
SP678	585	5-FAM-		2125.04
		BaLTA$r8EYAAQL$AAAAAa-NH2
SP679	586	5-FAM-		2259.09
		BaLTF$r8EYWAAL$AAAAAa-NH2
SP680	587	5-FAM-		2274.07
		BaLTF$r8EYWAQA$AAAAAa-NH2
SP681	588	5-FAM-		2159.03
		BaLTF$/r8EYWAQCbaS/SAA-NH2
SP682	589	5-FAM-BaLTA$r8EYWAQCba$SAA-		2054.97
		NH2
SP683	590	5-FAM-BaLTF$r8EYAAQCba$SAA-		2015.96
		NH2
SP684	591	5-FAM-BaLTA$r8EYAAQCba$SAA-		1939.92
		NH2
SP685	592	5-FAM-BaQSQQTF$r8NLWRLL$QN-		2495.23
		NH2
SP686	593	5-TAMRA-		2186.1
		BaLTF$r8EYWAQCba$SAA-NH2
SP687	594	5-TAMRA-		2110.07
		BaLTA$r8EYWAQCba$SAA-NH2
SP688	595	5-TAMRA-		2071.06
		BaLTF$r8EYAAQCba$SAA-NH2
SP689	596	5-TAMRA-		1995.03
		BaLTA$r8EYAAQCba$SAA-NH2
SP690	597	5-TAMRA-		2214.13
		BaLTF$/r8EYWAQCbaS/SAA-NH2
SP691	598	5-TAMRA-		2371.22
		BaLTF$r8EYWAQL$AAAAAa-NH2
SP692	599	5-TAMRA-		2295.19
		BaLTA$r8EYWAQL$AAAAAa-NH2
SP693	600	5-TAMRA-		2399.25
		BaLTF$/r8EYWAQLVAAAAAa-NH2
SP694	601	Ac-LTF$r8EYWCou7QCba$SAA-OH		1947.93
SP695	602	Ac-LTF$r8EYWCou7QCba$S-OH		1805.86
SP696	603	Ac-LTA$r8EYWCou7QCba$SAA-		1870.91
		NH2
SP697	604	Ac-LTF$r8EYACou7QCba$SAA-NH2		1831.9
SP698	605	Ac-LTA$r8EYACou7QCba$SAA-NH2		1755.87
SP699	606	Ac-LTF$/r8EYWCou7QCbaS/SAA-		1974.98
		NH2
SP700	607	Ac-LTF$r8EYWCou7QL$AAAAAa-		2132.06
		NH2
SP701	608	Ac-LTF$/r8EYWCou7QLVAAAAAa-		2160.09
		NH2
SP702	609	Ac-LTF$r8EYWCou7QL$AAAAA-		2062.01
		OH
SP703	610	Ac-LTF$r8EYWCou7QL$AAAA-OH		1990.97
SP704	611	Ac-LTF$r8EYWCou7QL$AAA-OH		1919.94
SP705	612	Ac-LTF$r8EYWCou7QL$AA-OH		1848.9
SP706	613	Ac-LTF$r8EYWCou7QL$A-OH		1777.86
SP707	614	Ac-LTF$r8EYWAQL$AAAASa-NH2	iso2		974.4		973.53
SP708	615	Ac-LTF$r8AYWAAL$AAAAAa-NH2	iso2	1814.01	908.82	1815.02	908.01	605.68
SP709	616	Biotin-		2184.14	1093.64	2185.15	1093.08	729.05
		BaLTF$r8EYWAQL$AAAAAa-NH2
SP710	617	Ac-LTF$r8HAWAQL$S-NH2	iso2	1505.84	754.43	1506.85	753.93	502.95
SP711	618	Ac-LTF$r8EYWAQCba$SA-NH2	iso2	1672.89	838.05	1673.9	837.45	558.64
SP712	619	Ac-LTF$r8HYWAQCba$SAA-NH2	iso2	1751.94	877.55	1752.95	876.98	584.99
SP713	620	Ac-LTF$r8SYWAQCba$SAA-NH2	iso2	1701.91	852.48	1702.92	851.96	568.31
SP714	621	Ac-LTF$r8RYWAQCba$SAA-NH2	iso2	1770.98	887.45	1771.99	886.5	591.33
SP715	622	Ac-LTF$r8KYWAQCba$SAA-NH2	iso2	1742.98	872.92	1743.99	872.5	582
SP716	623	Ac-LTF$r8EYWAQCba$AAA-NH2	iso2	1727.93	865.71	1728.94	864.97	576.98
SP717	624	Ac-LTF$r8EYWAQL$AAAAAaBaC-		2103.09	1053.12	2104.1	1052.55	702.04
		NH2
SP718	625	Ac-		2279.19	1141.46	2280.2	1140.6	760.74
		LTF$r8EYWAQL$AAAAAadPeg4C-
		NH2
SP719	626	Ac-LTA$r8AYWAAL$AAAAAa-NH2		1737.98	870.43	1738.99	870	580.33
SP720	627	Ac-LTF$r8AYAAAL$AAAAAa-NH2		1698.97	851	1699.98	850.49	567.33
SP721	628	5-FAM-		2201.09	1101.87	2202.1	1101.55	734.7
		BaLTF$r8AYWAAL$AAAAAa-NH2
SP722	629	Ac-LTA$r8AYWAQL$AAAAAa-NH2		1795	898.92	1796.01	898.51	599.34
SP723	630	Ac-LTF$r8AYAAQL$AAAAAa-NH2		1755.99	879.49	1757	879	586.34
SP724	631	Ac-		1807.97		1808.98	904.99	603.66
		LTF$rda6AYWAAL$da5AAAAAa-
		NH2
SP725	632	FITC-BaLTF$r8EYWAQL$AAAAAa-		2347.1	1174.49	2348.11	1174.56	783.37
		NH2
SP726	633	FITC-BaLTF$r8EYWAQCba$SAA-		2161.99	1082.35	2163	1082	721.67
		NH2
SP733	634	Ac-LTF$r8EYWAQL$EAAAAa-NH2		1987.05	995.03	1988.06	994.53	663.36
SP734	635	Ac-LTF$r8AYWAQL$EAAAAa-NH2		1929.04	966.35	1930.05	965.53	644.02
SP735	636	Ac-		2354.25	1178.47	2355.26	1178.13	785.76
		LTF$r8EYWAQL$AAAAAaBaKbio-
		NH2
SP736	637	Ac-LTF$r8AYWAAL$AAAAAa-NH2		1814.01	908.45	1815.02	908.01	605.68
SP737	638	Ac-LTF$r8AYAAAL$AAAAAa-NH2	iso2	1698.97	850.91	1699.98	850.49	567.33
SP738	639	Ac-LTF$r8AYAAQL$AAAAAa-NH2	iso2	1755.99	879.4	1757	879	586.34
SP739	640	Ac-LTF$r8EYWAQL$EAAAAa-NH2	iso2	1987.05	995.21	1988.06	994.53	663.36
SP740	641	Ac-LTF$r8AYWAQL$EAAAAa-NH2	iso2	1929.04	966.08	1930.05	965.53	644.02
SP741	642	Ac-LTF$r8EYWAQCba$SAAAAa-		1957.04	980.04	1958.05	979.53	653.35
		NH2
SP742	643	Ac-LTF$r8EYWAQLStAAA$r5AA-		2023.12	1012.83	2024.13	1012.57	675.38
		NH2
SP743	644	Ac-LTF$r8EYWAQL$A$AAA$A-		2108.17	1055.44	2109.18	1055.09	703.73
		NH2
SP744	645	Ac-LTF$r8EYWAQL$AA$AAA$A-		2179.21	1090.77	2180.22	1090.61	727.41
		NH2
SP745	646	Ac-LTF$r8EYWAQL$AAA$AAA$A-		2250.25	1126.69	2251.26	1126.13	751.09
		NH2
SP746	647	Ac-AAALTF$r8EYWAQL$AAA-OH		1930.02		1931.03	966.02	644.35
SP747	648	Ac-AAALTF$r8EYWAQL$AAA-NH2		1929.04	965.85	1930.05	965.53	644.02
SP748	649	Ac-AAAALTF$r8EYWAQL$AAA-		2000.08	1001.4	2001.09	1001.05	667.7
		NH2
SP749	650	Ac-AAAAALTF$r8EYWAQL$AAA-		2071.11	1037.13	2072.12	1036.56	691.38
		NH2
SP750	651	Ac-		2142.15		2143.16	1072.08	715.06
		AAAAAALTF$r8EYWAQL$AAA-
		NH2
SP751	652	Ac-LTF$rda6EYWAQCba$da6SAA-	iso2	1751.89	877.36	1752.9	876.95	584.97
		NH2
SP752	653	Ac-t$r5wya$r5f4CF3ekllr-NH2			844.25
SP753	654	Ac-tawy$r5nf4CF3e$r5llr-NH2			837.03
SP754	655	Ac-tawya$r5f4CF3ek$r5lr-NH2			822.97
SP755	656	Ac-tawyanf4CF3e$r5llr$r5a-NH2			908.35
SP756	657	Ac-t$s8wyanf4CF3e$r5llr-NH2			858.03
SP757	658	Ac-tawy$s8nf4CF3ekll$r5a-NH2			879.86
SP758	659	Ac-tawyaSs8f4CF3ekllr$r5a-NH2			936.38
SP759	660	Ac-tawy$s8naekll$r5a-NH2			844.25
SP760	661	5-FAM-Batawy$s8nf4CF3ekll$r5a-
		NH2
SP761	662	5-FAM-Batawy$s8naekll$r5a-NH2
SP762	663	Ac-tawy$s8nf4CF3eall$r5a-NH2
SP763	664	Ac-tawy$s8nf4CF3ekll$r5aaaaa-NH2
SP764	665	Ac-tawy$s8nf4CF3eall$r5aaaaa-NH2

Table 1a shows a selection of peptidomimetic macrocycles.

TABLE 1a

	SEQ					Calc	Calc	Calc
	ID			Exact	Found	(M + 1)/	(M + 2)/	(M + 3)/
SP	NO:	Sequence	Isomer	Mass	Mass		1	2	3

SP244	666	Ac-LTF$r8EF4coohWAQCba$SANleA-NH2		1885	943.59	1886.01	943.51	629.34
SP331	667	Ac-LTF$r8EYWAQL$AAAAAa-NH2	iso2	1929.04	966.08	1930.05	965.53	644.02
SP555	668	Ac-LTF$r8EY6clWAQL$AAAAAa-NH2		1963	983.28	1964.01	982.51	655.34
SP557	669	Ac-AAALTF$r8EYWAQL$AAAAAa-NH2		2142.15	1072.83	2143.16	1072.08	715.06
SP558	670	Ac-LTF34F2$r8EYWAQL$AAAAAa-NH2		1965.02	984.3	1966.03	983.52	656.01
SP562	671	Ac-LTF$r8EYWAQL$AAibAAAa-NH2		1943.06	973.11	1944.07	972.54	648.69
SP564	672	Ac-LTF$r8EYWAQL$AAAAibAa-NH2		1943.06	973.48	1944.07	972.54	648.69
SP566	673	Ac-LTF$r8EYWAQL$AAAAAiba-NH2	iso2	1943.06	973.38	1944.07	972.54	648.69
SP567	674	Ac-LTF$r8EYWAQL$AAAAAAib-NH2		1943.06	973.01	1944.07	972.54	648.69
SP572	675	Ac-LTF$r8EYWAQL$AAAAaa-NH2		1929.04	966.35	1930.05	965.53	644.02
SP573	676	Ac-LTF$r8EYWAQL$AAAAAA-NH2		1929.04	966.35	1930.05	965.53	644.02
SP578	677	Ac-LTF$r8EYWAQL$AAAAASar-NH2		1929.04	966.08	1930.05	965.53	644.02
SP551	678	Ac-LTF$r8EYWAQL$AAAAAa-OH	iso2	1930.02	965.89	1931.03	966.02	644.35
SP662	679	Ac-LTF$rda6AYWAQL$da5AAAAAa-NH2		1974.06	934.44		933.49
SP367	680	5-FAM-BaLTF$r8EYWAQCba$SAA-NH2		2131	1067.09	2132.01	1066.51	711.34
SP349	681	Ac-LTF$r8EF4coohWAQCba$AAAAAa-NH2	iso2	1969.04	986.06	1970.05	985.53	657.35
SP347	682	Ac-LTF$r8EYWAQCba$AAAAAa-NH2	iso2	1941.04	972.55	1942.05	971.53	648.02

Table 1b shows a further selection of peptidomimetic macrocycles.

TABLE 1b

	SEQ					Calc	Calc	Calc
	ID			Exact	Found	(M + 1)/	(M + 2)/	(M + 3)/
SP	NO:	Sequence	Isomer	Mass	Mass		1	2	3

SP581	683	Ac-TF$r8EYWAQL$AAAAAa-NH2	1815.96	929.85	1816.97	908.99	606.33
SP582	684	Ac-F$r8EYWAQL$AAAAAa-NH2	1714.91	930.92	1715.92	858.46	572.64
SP583	685	Ac-LVF$r8EYWAQL$AAAAAa-NH2	1927.06	895.12	1928.07	964.54	643.36
SP584	686	Ac-AAF$r8EYWAQL$AAAAAa-NH2	1856.98	859.51	1857.99	929.5	620
SP585	687	Ac-LTF$r8EYWAQL$AAAAa-NH2	1858	824.08	1859.01	930.01	620.34
SP586	688	Ac-LTF$r8EYWAQL$AAAa-NH2	1786.97	788.56	1787.98	894.49	596.66
SP587	689	Ac-LTF$r8EYWAQL$AAa-NH2	1715.93	1138.57	1716.94	858.97	572.98
SP588	690	Ac-LTF$r8EYWAQL$Aa-NH2	1644.89	1144.98	1645.9	823.45	549.3
SP589	691	Ac-LTF$r8EYWAQL$a-NH2	1573.85	1113.71	1574.86	787.93	525.62
SP590	692	Ac-LTF$r8AYWAQL$A-NH₂		758.97		758.93

Table 1d shows a list of selected peptidomimetic macrocycles.

TABLE 1d

Selected peptidomimetic macrocycles
derived from the MDM2/MDMX-binding helix of p53.

				IC₅₀	SJSA-1	Solubility
SP#	L	RT*	Ala (%)	MDM2 (nM)	EC₅₀(μM)**	(mg/mL)

590	12	74.2	25	140.7	6	≤1
68	15	91.5	33	29.02	1.12	3
315	17	≥100	47	30.77	0.18	4.5
317	18	≥100	50	10	0.1	5

*Normalized and calculated according to Example 11 (see table and equation).
**10% serum, 72 hr
L = length in amino acids;
RT = retention time;
Ala = alanine content

TABLE 1e

Peptidomimetic macrocycles that inhibit the MDM2/MDMX and
p53 interaction.

	SEQ		Calc.
	ID		(M +	Found
SP#	NO:	Sequence	2)/2	Mass

778	693	Ac-tawyanfekllr-NH₂	776.92	777.46
779	694	Ac-tawyanf4CF3ekllr-NH₂	810.91	811.41
752	695	Ac-t$r5wya$r5f4CF3ekllr-NH₂		844.25
753	696	Ac-tawy$r5nf4CF3e$r5llr-NH₂		837.03
754	697	Ac-tawya$r5f4CF3ek$r5lr-NH₂		822.97
755	698	Ac-tawyanf4CF3e$r5llr$r5a-NH₂		908.35
756	699	Ac-t$s8wyanf4CF3e$r5llr-NH₂		858.03
757	700	Ac-tawy$s8nf4CF3ekll$r5a-NH₂	878.97	879.86
758	701	Ac-tawya$s8f4CF3ekllr$r5a-NH₂		936.38
763	702	Ac-tawy$s8nf4CF3ekll$r5aaaaa-
		NH₂

TABLE 1f

Selected peptidomimetic macrocycles
that inhibit the MDM2/MDMX and p53 interaction.

				RT			SJSA-1
SP#	Ch	L	VH	(min)*	Ala (%)	Ki MDM2 (nM)	EC₅₀(μM)**

778	1	12	9.8	5.53	17	19251.34	>30
779	1	12	9.8	6.52	17	48.16	ND
757	0	13	6.3	7.99	15	2.92	1.5
763	0	17	6.7	8.74	35	10.9	0.34

*See Example 11 table
**10% serum, 72 hr
Ch = net charge;
L = length in amino acids;
VH = von Heijne;
RT = retention time;
Ala = alanine content

In some embodiments, the invention provides a peptidomimetic macrocycle that comprises an amino acid sequence that has at least 60%, 70%, 80%, 90%, 95%, 97%, or 100% identity to any one of the amino acid sequences in Table 1, 1a, 1b, 1c, 1e or 1f.
In the sequences shown above and elsewhere, the following abbreviations are used: “Nle” represents norleucine, “Aib” represents 2-aminoisobutyric acid, “Ac” represents acetyl, and “Pr” represents propionyl. Amino acids represented as “$” are alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon crosslinker comprising one double bond. Amino acids represented as “$r5” are alpha-Me R5-pentenyl-alanine olefin amino acids connected by an all-carbon comprising one double bond. Amino acids represented as “$s8” are alpha-Me S8-octenyl-alanine olefin amino acids connected by an all-carbon crosslinker comprising one double bond. Amino acids represented as “$r8” are alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon crosslinker comprising one double bond. “Ahx” represents an aminocyclohexyl linker. The crosslinkers are linear all-carbon crosslinker comprising eight or eleven carbon atoms between the alpha carbons of each amino acid. Amino acids represented as “$/” are alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker. Amino acids represented as “$/r5” are alpha-Me R5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker. Amino acids represented as “$/s8” are alpha-Me S8-octenyl-alanine olefin amino acids that are not connected by any crosslinker. Amino acids represented as “$/r8” are alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker. Amino acids represented as “Amw” are alpha-Me tryptophan amino acids. Amino acids represented as “Aml” are alpha-Me leucine amino acids. Amino acids represented as “Amf” are alpha-Me phenylalanine amino acids. Amino acids represented as “2ff” are 2-fluoro-phenylalanine amino acids. Amino acids represented as “3ff” are 3-fluoro-phenylalanine amino acids. Amino acids represented as “St” are amino acids comprising two pentenyl-alanine olefin side chains, each of which is crosslinked to another amino acid as indicated. Amino acids represented as “St//” are amino acids comprising two pentenyl-alanine olefin side chains that are not crosslinked. Amino acids represented as “% St” are amino acids comprising two pentenyl-alanine olefin side chains, each of which is crosslinked to another amino acid as indicated via fully saturated hydrocarbon crosslinks. Amino acids represented as “Ba” are beta-alanine. The lower-case character “e” or “z” within the designation of a crosslinked amino acid (e.g. “$er8” or “$zr8”) represents the configuration of the double bond (E or Z, respectively). In other contexts, lower-case letters such as “a” or “f” represent D amino acids (e.g. D-alanine, or D-phenylalanine, respectively). Amino acids designated as “NmW” represent N-methyltryptophan. Amino acids designated as “NmY” represent N-methyltyrosine. Amino acids designated as “NmA” represent N-methylalanine. “Kbio” represents a biotin group attached to the side chain amino group of a lysine residue. Amino acids designated as “Sar” represent sarcosine. Amino acids designated as “Cha” represent cyclohexyl alanine. Amino acids designated as “Cpg” represent cyclopentyl glycine. Amino acids designated as “Chg” represent cyclohexyl glycine. Amino acids designated as “Cba” represent cyclobutyl alanine. Amino acids designated as “F4I” represent 4-iodo phenylalanine. “7L” represents N15 isotopic leucine. Amino acids designated as “F3Cl” represent 3-chloro phenylalanine. Amino acids designated as “F4cooh” represent 4-carboxy phenylalanine. Amino acids designated as “F34F2” represent 3,4-difluoro phenylalanine. Amino acids designated as “6clW” represent 6-chloro tryptophan. Amino acids designated as “$rda6” represent alpha-Me R6-hexynyl-alanine alkynyl amino acids, crosslinked via a dialkyne bond to a second alkynyl amino acid. Amino acids designated as “$da5” represent alpha-Me S5-pentynyl-alanine alkynyl amino acids, wherein the alkyne forms one half of a dialkyne bond with a second alkynyl amino acid. Amino acids designated as “$ra9” represent alpha-Me R9-nonynyl-alanine alkynyl amino acids, crosslinked via an alkyne metathesis reaction with a second alkynyl amino acid. Amino acids designated as “$a6” represent alpha-Me S6-hexynyl-alanine alkynyl amino acids, crosslinked via an alkyne metathesis reaction with a second alkynyl amino acid. The designation “iso1” or “iso2” indicates that the peptidomimetic macrocycle is a single isomer.
Amino acids designated as “Cit” represent citrulline. Amino acids designated as “Cou4”, “Cou6”, “Cou7” and “Cou8”, respectively, represent the following structures:
In some embodiments, a peptidomimetic macrocycle is obtained in more than one isomer, for example due to the configuration of a double bond within the structure of the crosslink (E vs Z). Such isomers can or cannot be separable by conventional chromatographic methods. In some embodiments, one isomer has improved biological properties relative to the other isomer. In one embodiment, an E crosslink olefin isomer of a peptidomimetic macrocycle has better solubility, better target affinity, better in vivo or in vitro efficacy, higher helicity, or improved cell permeability relative to its Z counterpart. In another embodiment, a Z crosslink olefin isomer of a peptidomimetic macrocycle has better solubility, better target affinity, better in vivo or in vitro efficacy, higher helicity, or improved cell permeability relative to its E counterpart.
Table 1c shows exemplary peptidomimetic macrocycle:

TABLE 1c

SEQ ID NO:	Structure

162	Chemical Formula: C₈₇H₁₂₅N₁₇O₂₁ Exact Mass: 1743.92 Molecular Weight: 1745.02 Ac-L T F $er8EYWAQCba$eSAA-NH2

123	Chemical Formula: C₈₅H₁₂₅N₁₇O₁₉ Exact Mass: 1687.93 Molecular Weight: 1689.00 Ac-L T F $er8AYWAQhL$eSAA-NH2

122	Chemical Formula: C₈₅H₁₂₅N₁₇O₁₉ Exact Mass: 1687.93 Molecular Weight: 1689.00 Ac-L T F $zr8AYWAQhL$zSAA-NH2

107	Chemical Formula: C₈₄H₁₂₂ClN₁₇O₁₉ Exact Mass: 1707.88 Molecular Weight: 1709.42 Ac-L T F $er8AY6clWAQL$eSAA-NH2

396	Chemical Formula: C₉₁H₁₃₆N₁₈O₁₉ Exact Mass: 1785.02 Molecular Weight: 1786.16 Ac-L T F $er8AYAmwAQL$eAA Nle A-NH2

339	Chemical Formula: C₉₅H₁₄₀N₂₀O₂₃ Exact Mass: 1929.04 Molecular Weight: 1930.04 Ac-L T F $er8EYWAQL$eAAAAAa-NH2

453	Chemical Formula: C₉₅H₁₄₂N₂₀O₂₃ Exact Mass: 1931.06 Molecular Weight: 1932.26 Ac-L T F %r8EYWAQL %AAAAAa-NH2

359	Chemical Formula: C₉₆H₁₄₀N₂₀O₂₄ Exact Mass: 1957.03 Molecular Weight: 1958.26 Ac-L T F $er8EYWSQCba$AAAAAa-NH2

79	Chemical Formula: C₉₀H₁₃₄N₁₈O₁₉ Exact Mass: 1771.01 Molecular Weight: 1772.14 Ac-L T F $er8AYWAQL$eAA I a-NH2

77	Chemical Formula: C₉₀H₁₃₄N₁₈O₁₉ Exact Mass: 1771.01 Molecular Weight: 1772.14 Ac-L T F $er8AYWAQL$eAA NleA-NH2

15	Chemical Formula: C₉₀H₁₂₇N₁₇O₁₉ Exact Mass: 1747.95 Molecular Weight: 1751.07 Ac-L T F $r8AYWAQL$SA F-NH2

168	Chemical Formula: C₈₇H₁₂₅F₂N₁₇O₂₁ Exact Mass: 1781.92 Molecular Weight: 1781.92 Ac-L T F43F2$er8EYWAQhL$eSAA-NH2

323	Chemical Formula: C₉₃H₁₃₈N₂₀O₂₁ Exact Mass: 1871.03 Molecular Weight: 1872.21 Ac-L T F $er8AYWAQL$eAAAAAa-NH2

257	Chemical Formula: C₉₄H₁₃₆N₁₈O₂₂ Exact Mass: 1869.01 Molecular Weight: 1870.19 Ac-L T F $er8EF4coohWAQCba$e AA-I-a-NH2

445	Chemical Formula: C₉₅H₁₄₃N₂₁O₂₁ Exact Mass: 1914.08 Molecular Weight: 1915.28 Dmaac-L T F $er8AYWAQL$eAAAAAa-NH2

357	Chemical Formula: C₉₇H₁₄₀N₂₀O₂₄ Exact Mass: 1969.03 Molecular Weight: 1970.27 Ac-L T F $er8EF4coohWAQCba$eAAAAAa-NH2

463	Chemical Formula: C₉₅H₁₃₉ClN₂₀O₂₃ Exact Mass: 1963.00 Molecular Weight: 1964.69 Ac-L T F $er8EY6clWAQL$eAAAAAa-NH2

465	Chemical Formula: C₁₀₄H₁₅₅N₂₃O₂₆ Exact Mass: 2142.15 Molecular Weight: 2143.48 Ac-AAA L T F $er8EYWAQL$eAAAAAa-NH2

466	Chemical Formula: C₉₅H₁₃₈F₂N₂₀O₂₃ Exact Mass: 1965.02 Molecular Weight: 1966.23 Ac-L T F34F2 $er8EYWAQL$eAAAAAa-NH2

375	5-FAM-Ba L T F $er8EYWAQCba$eSAA-NH2

470	Chemical Formula: C₉₆H₁₄₂N₂₀O₂₃ Exact Mass: 1943.06 Molecular Weight: 1944.27 Ac-L T F$er8EYWAQL$eAAib AAA a-NH2

472	Chemical Formula: C₉₆H₁₄₂N₂₀O₂₃ Exact Mass: 1943.06 Molecular Weight: 1944.27 Ac-L T F$er8EYWAQL$eAAAAib A a-NH2

474

475	Chemical Formula: C₉₆H₁₄₂N₂₀O₂₃ Exact Mass: 1943.06 Molecular Weight: 1944.27 Ac-L T F $er8EYWAQL$eAAAAAAib-NH2

480	Chemical Formula: C₉₅H₁₄₀N₂₀O₂₃ Exact Mass: 1929.04 Molecular Weight: 1930.25 Ac-L T F $er8EYWAQL$eAAAA a a-NH2

481	Chemical Formula: C₉₅H₁₄₀N₂₀O₂₃ Exact Mass: 1929.04 Molecular Weight: 1930.25 Ac-L T F $er8EYWAQL$eAAAAAA-NH2

486	Chemical Formula: C₉₅H₁₄₀N₂₀O₂₃ Exact Mass: 1929.04 Molecular Weight: 1930.25 Ac-L T F $er8EYWAQL$eAAAAA Sar-NH2

571	Chemical Formula: C₉₅H₁₃₄N₂₀O₂₃ Exact Mass: 1922.99 Molecular Weight: 1924.20 Ac-LTF$rda6EYWAQL$da5AAAAAa-NH2

569	Chemical Formula: C₉₅H₁₃₄N₂₀O₂₃ Exact Mass: 1922.99 Molecular Weight: 1924.20 Ac-L T F $rda6EYWAQL$da5AAAAA a-NH2

703	Chemical Formula: C₉₆H₁₃₆N₂₀O₂₃ Exact Mass: 1937.01 Molecular Weight: 1938.23

In some embodiments, peptidomimetic macrocycles exclude peptidomimetic macrocycles shown in Table 2a:

TABLE 2a

Number	SEQ ID NO:	Sequence

1	704	L$r5QETFSD$s8WKLLPEN

2	705	LSQ$r5TFSDLW$s8LLPEN

3	706	LSQE$r5FSDLWK$s8LPEN

4	707	LSQET$r5SDLWKL$s8PEN

5	708	LSQETF$r5DLWKLL$s8EN

6	709	LXQETFS$r5LWKLLP$s8N

7	710	LSQETFSD$r5WKLLPE$s8

8	711	LSQQTF$r5DLWKLL$s8EN

9	712	LSQETF$r5DLWKLL$s8QN

10	713	LSQQTF$r5DLWKLL$s8QN

11	714	LSQETF$r5NLWKLL$s8QN

12	715	LSQQTF$r5NLWKLL$s8QN

13	716	LSQQTF$r5NLWRLL$s8QN

14	717	QSQQTF$r5NLWKLL$s8QN

15	718	QSQQTF$r5NLWRLL$s8QN

16	719	QSQQTA$r5NLWRLL$s8QN

17	720	L$r8QETFSD$WKLLPEN

18	721	LSQ$r8TFSDLW$LLPEN

19	722	LSQE$r8FSDLWK$LPEN

20	723	LSQET$r8SDLWKL$PEN

21	724	LSQETF$r8DLWKLL$EN

22	725	LXQETFS$r8LWKLLP$N

23	726	LSQETFSD$r8WKLLPE$

24	727	LSQQTF$r8DLWKLL$EN

25	728	LSQETF$r8DLWKLL$QN

26	729	LSQQTF$r8DLWKLL$QN

27	730	LSQETF$r8NLWKLL$QN

28	731	LSQQTF$r8NLWKLL$QN

29	732	LSQQTF$r8NLWRLL$QN

30	733	QSQQTF$r8NLWKLL$QN

31	734	QSQQTF$r8NLWRLL$QN

32	735	QSQQTA$r8NLWRLL$QN

33	736	QSQQTF$r8NLWRKK$QN

34	737	QQTF$r8DLWRLL$EN

35	738	QQTF$r8DLWRLL$

36	739	LSQQTF$DLW$LL

37	740	QQTF$DLW$LL

38	741	QQTA$r8DLWRLL$EN

39	742	QSQQTF$r5NLWRLL$s8QN
		(dihydroxylated olefin)

40	743	QSQQTA$r5NLWRLL$s8QN
		(dihydroxylated olefin)

41	744	QSQQTF$r8DLWRLL$QN

42	745	QTF$r8NLWRLL$

43	746	QSQQTF$NLW$LLPQN

44	747	QS$QTF$NLWRLLPQN

45	748	$TFS$LWKLL

46	749	ETF$DLW$LL

47	750	QTF$NLW$LL

48	751	$SQE$FSNLWKLL

In Table 2a, X represents S or any amino acid. Peptides shown can comprise an N-terminal capping group such as acetyl or an additional linker such as beta-alanine between the capping group and the start of the peptide sequence.
In some embodiments, peptidomimetic macrocycles do not comprise a peptidomimetic macrocycle structure as shown in Table 2a.
In other embodiments, peptidomimetic macrocycles exclude peptidomimetic macrocycles shown in Table 2b:

TABLE 2b

	SEQ				Observed
	ID		Exact		mass
Number	NO:	Sequence	Mass	M + 2	(m/e)

1	752	Ac-LSQETF$r8DLWKLL$EN-NH2	2068.13	1035.07	1035.36
2	753	Ac-LSQETF$r8NLWKLL$QN-NH2	2066.16	1034.08	1034.31
3	754	Ac-LSQQTF$r8NLWRLL$QN-NH2	2093.18	1047.59	1047.73
4	755	Ac-QSQQTF$r8NLWKLL$QN-NH2	2080.15	1041.08	1041.31
5	756	Ac-QSQQTF$r8NLWRLL$QN-NH2	2108.15	1055.08	1055.32
6	757	Ac-QSQQTA$r8NLWRLL$QN-NH2	2032.12	1017.06	1017.24
7	758	Ac-QAibQQTF$r8NLWRLL$QN-NH2	2106.17	1054.09	1054.34
8	759	Ac-QSQQTFSNLWRLLPQN-NH2	2000.02	1001.01	1001.26
9	760	Ac-QSQQTF$/r8NLWRLLS/QN-NH2	2136.18	1069.09	1069.37
10	761	Ac-QSQAibTF$r8NLWRLL$QN-NH2	2065.15	1033.58	1033.71
11	762	Ac-QSQQTF$r8NLWRLL$AN-NH2	2051.13	1026.57	1026.70
12	763	Ac-ASQQTF$r8NLWRLL$QN-NH2	2051.13	1026.57	1026.90
13	764	Ac-QSQQTF$r8ALWRLL$QN-NH2	2065.15	1033.58	1033.41
14	765	Ac-QSQETF$r8NLWRLL$QN-NH2	2109.14	1055.57	1055.70
15	766	Ac-RSQQTF$r8NLWRLL$QN-NH2	2136.20	1069.10	1069.17
16	767	Ac-RSQQTF$r8NLWRLL$EN-NH2	2137.18	1069.59	1069.75
17	768	Ac-LSQETFSDLWKLLPEN-NH2	1959.99	981.00	981.24
18	769	Ac-QSQ$TFS$LWRLLPQN-NH2	2008.09	1005.05	1004.97
19	770	Ac-QSQQ$FSN$WRLLPQN-NH2	2036.06	1019.03	1018.86
20	771	Ac-QSQQT$SNL$RLLPQN-NH2	1917.04	959.52	959.32
21	772	Ac-QSQQTF$NLW$LLPQN-NH2	2007.06	1004.53	1004.97
22	773	Ac-RTQATF$r8NQWAibANle$TNAibTR-	2310.26	1156.13	1156.52
		NH2
23	774	Ac-QSQQTF$r8NLWRLL$RN-NH2	2136.20	1069.10	1068.94
24	775	Ac-QSQRTF$r8NLWRLL$QN-NH2	2136.20	1069.10	1068.94
25	776	Ac-QSQQTF$r8NNleWRLL$QN-NH2	2108.15	1055.08	1055.44
26	777	Ac-QSQQTF$r8NLWRNleL$QN-NH2	2108.15	1055.08	1055.84
27	778	Ac-QSQQTF$r8NLWRLNle$QN-NH2	2108.15	1055.08	1055.12
28	779	Ac-QSQQTY$r8NLWRLL$QN-NH2	2124.15	1063.08	1062.92
29	780	Ac-RAibQQTF$r8NLWRLL$QN-NH2	2134.22	1068.11	1068.65
30	781	Ac-MPRFMDYWEGLN-NH2	1598.70	800.35	800.45
31	782	Ac-RSQQRF$r8NLWRLL$QN-NH2	2191.25	1096.63	1096.83
32	783	Ac-QSQQRF$r8NLWRLL$QN-NH2	2163.21	1082.61	1082.87
33	784	Ac-RAibQQRF$r8NLWRLL$QN-NH2	2189.27	1095.64	1096.37
34	785	Ac-RSQQRF$r8NFWRLL$QN-NH2	2225.23	1113.62	1114.37
35	786	Ac-RSQQRF$r8NYWRLL$QN-NH2	2241.23	1121.62	1122.37
36	787	Ac-RSQQTF$r8NLWQLL$QN-NH2	2108.15	1055.08	1055.29
37	788	Ac-QSQQTF$r8NLWQAmlL$QN-NH2	2094.13	1048.07	1048.32
38	789	Ac-QSQQTF$r8NAmlWRLL$QN-NH2	2122.17	1062.09	1062.35
39	790	Ac-NlePRF$r8DYWEGL$QN-NH2	1869.98	935.99	936.20
40	791	Ac-NlePRF$r8NYWRLL$QN-NH2	1952.12	977.06	977.35
41	792	Ac-RF$r8NLWRLL$Q-NH2	1577.96	789.98	790.18
42	793	Ac-QSQQTF$r8N2ffWRLL$QN-NH2	2160.13	1081.07	1081.40
43	794	Ac-QSQQTF$r8N3ffWRLL$QN-NH2	2160.13	1081.07	1081.34
44	795	Ac-QSQQTF#r8NLWRLL#QN-NH2	2080.12	1041.06	1041.34
45	796	Ac-RSQQTA$r8NLWRLL$QN-NH2	2060.16	1031.08	1031.38
46	797	Ac-QSQQTF%r8NLWRLL%QN-NH2	2110.17	1056.09	1056.55
47	798	HepQSQ$TFSNLWRLLPQN-NH2	2051.10	1026.55	1026.82
48	799	HepQSQ$TF$r8NLWRLL$QN-NH2	2159.23	1080.62	1080.89
49	800	Ac-QSQQTF$r8NL6clWRLL$QN-NH2	2142.11	1072.06	1072.35
50	801	Ac-QSQQTF$r8NLMe6clwRLL$QN-NH2	2156.13	1079.07	1079.27
51	802	Ac-LTFEHYWAQLTS-NH2	1535.74	768.87	768.91
52	803	Ac-LTF$HYW$QLTS-NH2	1585.83	793.92	794.17
53	804	Ac-LTFE$YWA$LTS-NH2	1520.79	761.40	761.67
54	805	Ac-LTF$zr8HYWAQL$zS-NH2	1597.87	799.94	800.06
55	806	Ac-LTF $r8HYWRQL$S-NH2	1682.93	842.47	842.72
56	807	Ac-QS$QTFStNLWRLL$s8QN-NH2	2145.21	1073.61	1073.90
57	808	Ac-QSQQTASNLWRLLPQN-NH2	1923.99	963.00	963.26
58	809	Ac-QSQQTA$/r8NLWRLLS/QN-NH2	2060.15	1031.08	1031.24
59	810	Ac-ASQQTF$/r8NLWRLLS/QN-NH2	2079.16	1040.58	1040.89
60	811	Ac-$SQQ$FSNLWRLLAibQN-NH2	2009.09	1005.55	1005.86
61	812	Ac-QS$QTF$NLWRLLAibQN-NH2	2023.10	1012.55	1012.79
62	813	Ac-QSQQ$FSN$WRLLAibQN-NH2	2024.06	1013.03	1013.31
63	814	Ac-QSQQTF$NLW$LLAibQN-NH2	1995.06	998.53	998.87
64	815	Ac-QSQQTFS$LWR$LAibQN-NH2	2011.06	1006.53	1006.83
65	816	Ac-QSQQTFSNLW$LLA$N-NH2	1940.02	971.01	971.29
66	817	Ac-$/SQQ$/FSNLWRLLAibQN-NH2	2037.12	1019.56	1019.78
67	818	Ac-QS$/QTFS/NLWRLLAibQN-NH2	2051.13	1026.57	1026.90
68	819	Ac-QSQQ$/FSN$/WRLLAibQN-NH2	2052.09	1027.05	1027.36
69	820	Ac-QSQQTFS/NLW$/LLAibQN-NH2	2023.09	1012.55	1013.82
70	821	Ac-QSQ$TFS$LWRLLAibQN-NH2	1996.09	999.05	999.39
71	822	Ac-QSQ/TFS$/LWRLLAibQN-NH2	2024.12	1013.06	1013.37
72	823	Ac-QS$/QTFSt//NLWRLL$/s8QN-NH2	2201.27	1101.64	1102.00
73	824	Ac-$r8SQQTFS$LWRLLAibQN-NH2	2038.14	1020.07	1020.23
74	825	Ac-QSQ$r8TFSNLW$LLAibQN-NH2	1996.08	999.04	999.32
75	826	Ac-QSQQTFS$r8LWRLLA$N-NH2	2024.12	1013.06	1013.37
76	827	Ac-QS$r5QTFStNLW$LLAibQN-NH2	2032.12	1017.06	1017.39
77	828	Ac-$/r8SQQTFSS/LWRLLAibQN-NH2	2066.17	1034.09	1034.80
78	829	Ac-QSQ$/r8TFSNLM/LLAibQN-NH2	2024.11	1013.06	1014.34
79	830	Ac-QSQQTFS$/r8LWRLLA$/N-NH2	2052.15	1027.08	1027.16
80	831	Ac-QS$/r5QTFSt//NLW$/LLAibQN-NH2	2088.18	1045.09	1047.10
81	832	Ac-QSQQTFSNLWRLLAibQN-NH2	1988.02	995.01	995.31
82	833	Hep/QSQS/TF$/r8NLWRLLS/QN-NH2	2215.29	1108.65	1108.93
83	834	Ac-ASQQTF$r8NLRWLL$QN-NH2	2051.13	1026.57	1026.90
84	835	Ac-QSQQTF$/r8NLWRLLS/Q-NH2	2022.14	1012.07	1012.66
85	836	Ac-QSQQTF$r8NLWRLL$Q-NH2	1994.11	998.06	998.42
86	837	Ac-AAARAA$r8AAARAA$AA-NH2	1515.90	758.95	759.21
87	838	Ac-LTFEHYWAQLTSA-NH2	1606.78	804.39	804.59
88	839	Ac-LTF$r8HYWAQL$SA-NH2	1668.90	835.45	835.67
89	840	Ac-ASQQTFSNLWRLLPQN-NH2	1943.00	972.50	973.27
90	841	Ac-QS$QTFStNLW$r5LLAibQN-NH2	2032.12	1017.06	1017.30
91	842	Ac-QSQQTFAibNLWRLLAibQN-NH2	1986.04	994.02	994.19
92	843	Ac-QSQQTFNleNLWRLLNleQN-NH2	2042.11	1022.06	1022.23
93	844	Ac-QSQQTF$/r8NLWRLLAibQN-NH2	2082.14	1042.07	1042.23
94	845	Ac-QSQQTF$/r8NLWRLLNleQN-NH2	2110.17	1056.09	1056.29
95	846	Ac-QSQQTFAibNLWRLLS/QN-NH2	2040.09	1021.05	1021.25
96	847	Ac-QSQQTFNleNLWRLL$/QN-NH2	2068.12	1035.06	1035.31
97	848	Ac-QSQQTF%r8NL6clWRNleL%QN-NH2	2144.13	1073.07	1073.32
98	849	Ac-QSQQTF%r8NLMe6clWRLL%QN-NH2	2158.15	1080.08	1080.31
101	850	Ac-FNle$YWE$L-NH2	1160.63	—	1161.70
102	851	Ac-F$r8AYWELL$A-NH2	1344.75	—	1345.90
103	852	Ac-F$r8AYWQLL$A-NH2	1343.76	—	1344.83
104	853	Ac-NlePRF$r8NYWELL$QN-NH2	1925.06	963.53	963.69
105	854	Ac-NlePRF$r8DYWRLL$QN-NH2	1953.10	977.55	977.68
106	855	Ac-NlePRF$r8NYWRLL$Q-NH2	1838.07	920.04	920.18
107	856	Ac-NlePRF$r8NYWRLL$-NH2	1710.01	856.01	856.13
108	857	Ac-QSQQTF$r8DLWRLL$QN-NH2	2109.14	1055.57	1055.64
109	858	Ac-QSQQTF$r8NLWRLL$EN-NH2	2109.14	1055.57	1055.70
110	859	Ac-QSQQTF$r8NLWRLL$QD-NH2	2109.14	1055.57	1055.64
111	860	Ac-QSQQTF$r8NLWRLL$S-NH2	1953.08	977.54	977.60
112	861	Ac-ESQQTF$r8NLWRLL$QN-NH2	2109.14	1055.57	1055.70
113	862	Ac-LTF$r8NLWRNleL$Q-NH2	1635.99	819.00	819.10
114	863	Ac-LRF$r8NLWRNleL$Q-NH2	1691.04	846.52	846.68
115	864	Ac-QSQQTF$r8NWWRNleL$QN-NH2	2181.15	1091.58	1091.64
116	865	Ac-QSQQTF$r8NLWRNleL$Q-NH2	1994.11	998.06	998.07
117	866	Ac-QTF$r8NLWRNleL$QN-NH2	1765.00	883.50	883.59
118	867	Ac-NlePRF$r8NWWRLL$QN-NH2	1975.13	988.57	988.75
119	868	Ac-NlePRF$r8NWWRLL$A-NH2	1804.07	903.04	903.08
120	869	Ac-TSFAEYWNLLNH2	1467.70	734.85	734.90
121	870	Ac-QTF$r8HWWSQL$S-NH2	1651.85	826.93	827.12
122	871	Ac-FM$YWE$L-NH2	1178.58	—	1179.64
123	872	Ac-QTFEHWWSQLLS-NH2	1601.76	801.88	801.94
124	873	Ac-QSQQTF$r8NLAmwRLNle$QN-NH2	2122.17	1062.09	1062.24
125	874	Ac-FMAibY6clWEAc3cL-NH2	1130.47	—	1131.53
126	875	Ac-FNle$Y6clWE$L-NH2	1194.59	—	1195.64
127	876	Ac-F$zr8AY6clWEAc3cL$z-NH2	1277.63	639.82	1278.71
128	877	Ac-F$r8AY6clWEAc3cL$A-NH2	1348.66	—	1350.72
129	878	Ac-NlePRF$r8NY6clWRLL$QN-NH2	1986.08	994.04	994.64
130	879	Ac-AF$r8AAWALA$A-NH2	1223.71	—	1224.71
131	880	Ac-TF$r8AAWRLA$Q-NH2	1395.80	698.90	399.04
132	881	Pr-TF$r8AAWRLA$Q-NH2	1409.82	705.91	706.04
133	882	Ac-QSQQTF%r8NLWRNleL%QN-NH2	2110.17	1056.09	1056.22
134	883	Ac-LTF%r8HYWAQL%SA-NH2	1670.92	836.46	836.58
135	884	Ac-NlePRF%r8NYWRLL%QN-NH2	1954.13	978.07	978.19
136	885	Ac-NlePRF%r8NY6clWRLL%QN-NH2	1988.09	995.05	995.68
137	886	Ac-LTF%r8HY6clWAQL%S-NH2	1633.84	817.92	817.93
138	887	Ac-QS%QTF%StNLWRLL%s8QN-NH2	2149.24	1075.62	1075.65
139	888	Ac-LTF%r8HY6clWRQL%S-NH2	1718.91	860.46	860.54
140	889	Ac-QSQQTF%r8NL6clWRLL%QN-NH2	2144.13	1073.07	1073.64
141	890	Ac-%r8SQQTFS%LWRLLAibQN-NH2	2040.15	1021.08	1021.13
142	891	Ac-LTF%r8HYWAQL%S-NH2	1599.88	800.94	801.09
143	892	Ac-TSF%r8QYWNLL%P-NH2	1602.88	802.44	802.58
147	893	Ac-LTFEHYWAQLTS-NH2	1535.74	768.87	769.5
152	894	Ac-F$er8AY6clWEAc3cL$e-NH2	1277.63	639.82	1278.71
153	895	Ac-AF$r8AAWALA$A-NH2	1277.63	639.82	1277.84
154	896	Ac-TF$r8AAWRLA$Q-NH2	1395.80	698.90	699.04
155	897	Pr-TF$r8AAWRLA$Q-NH2	1409.82	705.91	706.04
156	898	Ac-LTF$er8HYWAQLSeS-NH2	1597.87	799.94	800.44
159	899	Ac-CCPGCCBaQSQQTF$r8NLWRLL$QN-
		NH2	2745.30	1373.65	1372.99
160	900	Ac-
		CCPGCCBaQSQQTA$r8NLWRLL$QN-
		NH2	2669.27	1335.64	1336.09
161	901	Ac-CCPGCCBaNlePRF$r8NYWRLL$QN-
		NH2	2589.26	1295.63	1296.2
162	902	Ac-LTF$/r8HYWAQL$/S-NH2	1625.90	813.95	814.18
163	903	Ac-F%r8HY6clWRAc3cL%-NH2	1372.72	687.36	687.59
164	904	Ac-QTF%r8HWWSQL%S-NH2	1653.87	827.94	827.94
165	905	Ac-LTA$r8HYWRQL$S-NH2	1606.90	804.45	804.66
166	906	Ac-Q$r8QQTFSN$WRLLAibQN-NH2	2080.12	1041.06	1041.61
167	907	Ac-QSQQ$r8FSNLWR$LAibQN-NH2	2066.11	1034.06	1034.58
168	908	Ac-F$r8AYWEAc3cL$A-NH2	1314.70	658.35	1315.88
169	909	Ac-F$r8AYWEAc3cL$S-NH2	1330.70	666.35	1331.87
170	910	Ac-F$r8AYWEAc3cL$Q-NH2	1371.72	686.86	1372.72
171	911	Ac-F$r8AYWEAibL$S-NH2	1332.71	667.36	1334.83
172	912	Ac-F$r8AYWEAL$S-NH2	1318.70	660.35	1319.73
173	913	Ac-F$r8AYWEQL$S-NH2	1375.72	688.86	1377.53
174	914	Ac-F$r8HYWEQL$S-NH2	1441.74	721.87	1443.48
175	915	Ac-F$r8HYWAQL$S-NH2	1383.73	692.87	1385.38
176	916	Ac-F$r8HYWAAc3cL$S-NH2	1338.71	670.36	1340.82
177	917	Ac-F$r8HYWRAc3cL$S-NH2	1423.78	712.89	713.04
178	918	Ac-F$r8AYWEAc3cL#A-NH2	1300.69	651.35	1302.78
179	919	Ac-NlePTF%r8NYWRLL%QN-NH2	1899.08	950.54	950.56
180	920	Ac-TF$r8AAWRAL$Q-NH2	1395.80	698.90	699.13
181	921	Ac-TSF%r8HYWAQL%S-NH2	1573.83	787.92	787.98
184	922	Ac-F%r8AY6clWEAc3cL%A-NH2	1350.68	676.34	676.91
185	923	Ac-LTF$r8HYWAQI$S-NH2	1597.87	799.94	800.07
186	924	Ac-LTF$r8HYWAQNle$S-NH2	1597.87	799.94	800.07
187	925	Ac-LTF$r8HYWAQL$A-NH2	1581.87	791.94	792.45
188	926	Ac-LTF$r8HYWAQL$Abu-NH2	1595.89	798.95	799.03
189	927	Ac-LTF$r8HYWAbuQL$S-NH2	1611.88	806.94	807.47
190	928	Ac-LTF$er8AYWAQL$e-NH2	1531.84	766.92	766.96
191	929	Ac-LAF$r8HYWAQL$S-NH2	1567.86	784.93	785.49
192	930	Ac-LAF$r8AYWAQL$S-NH2	1501.83	751.92	752.01
193	931	Ac-LTF$er8AYWAQL$eA-NH2	1515.85	758.93	758.97
194	932	Ac-LAF$r8AYWAQL$A-NH2	1485.84	743.92	744.05
195	933	Ac-LTF$r8NLWANleL$Q-NH2	1550.92	776.46	776.61
196	934	Ac-LTF$r8NLWANleL$A-NH2	1493.90	747.95	1495.6
197	935	Ac-LTF$r8ALWANleL$Q-NH2	1507.92	754.96	755
198	936	Ac-LAF$r8NLWANleL$Q-NH2	1520.91	761.46	761.96
199	937	Ac-LAF$r8ALWANleL$A-NH2	1420.89	711.45	1421.74
200	938	Ac-A$r8AYWEAc3cL$A-NH2	1238.67	620.34	1239.65
201	939	Ac-F$r8AYWEAc3cL$AA-NH2	1385.74	693.87	1386.64
202	940	Ac-F$r8AYWEAc3cL$Abu-NH2	1328.72	665.36	1330.17
203	941	Ac-F$r8AYWEAc3cL$Nle-NH2	1356.75	679.38	1358.22
204	942	Ac-F$r5AYWEAc3cL$s8A-NH2	1314.70	658.35	1315.51
205	943	Ac-F$AYWEAc3cL$r8A-NH2	1314.70	658.35	1315.66
206	944	Ac-F$r8AYWEAc3cI$A-NH2	1314.70	658.35	1316.18
207	945	Ac-F$r8AYWEAc3cNle$A-NH2	1314.70	658.35	1315.66
208	946	Ac-F$r8AYWEAmlL$A-NH2	1358.76	680.38	1360.21
209	947	Ac-F$r8AYWENleL$A-NH2	1344.75	673.38	1345.71
210	948	Ac-F$r8AYWQAc3cL$A-NH2	1313.72	657.86	1314.7
211	949	Ac-F$r8AYWAAc3cL$A-NH2	1256.70	629.35	1257.56
212	950	Ac-F$r8AYWAbuAc3cL$A-NH2	1270.71	636.36	1272.14
213	951	Ac-F$r8AYWNleAc3cL$A-NH2	1298.74	650.37	1299.67
214	952	Ac-F$r8AbuYWEAc3cL$A-NH2	1328.72	665.36	1329.65
215	953	Ac-F$r8NleYWEAc3cL$A-NH2	1356.75	679.38	1358.66
216	954	5-FAM-BaLTFEHYWAQLTS-NH2	1922.82	962.41	962.87
217	955	5-FAM-BaLTF%r8HYWAQL%S-NH2	1986.96	994.48	994.97
218	956	Ac-LTF$r8HYWAQhL$S-NH2	1611.88	806.94	807
219	957	Ac-LTF$r8HYWAQTle$S-NH2	1597.87	799.94	799.97
220	958	Ac-LTF$r8HYWAQAdm$S-NH2	1675.91	838.96	839.09
221	959	Ac-LTF$r8HYWAQhCha$S-NH2	1651.91	826.96	826.98
222	960	Ac-LTF$r8HYWAQCha$S-NH2	1637.90	819.95	820.02
223	961	Ac-LTF$r8HYWAc6cQL$S-NH2	1651.91	826.96	826.98
224	962	Ac-LTF$r8HYWAc5cQL$S-NH2	1637.90	819.95	820.02
225	963	Ac-LThF$r8HYWAQL$S-NH2	1611.88	806.94	807
226	964	Ac-LTIgl$r8HYWAQL$S-NH2	1625.90	813.95	812.99
227	965	Ac-LTF$r8HYWAQChg$S-NH2	1623.88	812.94	812.99
228	966	Ac-LTF$r8HYWAQF$S-NH2	1631.85	816.93	816.99
229	967	Ac-LTF$r8HYWAQIgl$S-NH2	1659.88	830.94	829.94
230	968	Ac-LTF$r8HYWAQCba$S-NH2	1609.87	805.94	805.96
231	969	Ac-LTF$r8HYWAQCpg$S-NH2	1609.87	805.94	805.96
232	970	Ac-LTF$r8HhYWAQL$S-NH2	1611.88	806.94	807
233	971	Ac-F$r8AYWEAc3chL$A-NH2	1328.72	665.36	665.43
234	972	Ac-F$r8AYWEAc3cTle$A-NH2	1314.70	658.35	1315.62
235	973	Ac-F$r8AYWEAc3cAdm$A-NH2	1392.75	697.38	697.47
236	974	Ac-F$r8AYWEAc3chCha$A-NH2	1368.75	685.38	685.34
237	975	Ac-F$r8AYWEAc3cCha$A-NH2	1354.73	678.37	678.38
238	976	Ac-F$r8AYWEAc6cL$A-NH2	1356.75	679.38	679.42
239	977	Ac-F$r8AYWEAc5cL$A-NH2	1342.73	672.37	672.46
240	978	Ac-hF$r8AYWEAc3cL$A-NH2	1328.72	665.36	665.43
241	979	Ac-Igl$r8AYWEAc3cL$A-NH2	1342.73	672.37	671.5
243	980	Ac-F$r8AYWEAc3cF$A-NH2	1348.69	675.35	675.35
244	981	Ac-F$r8AYWEAc3cIgl$A-NH2	1376.72	689.36	688.37
245	982	Ac-F$r8AYWEAc3cCba$A-NH2	1326.70	664.35	664.47
246	983	Ac-F$r8AYWEAc3cCpg$A-NH2	1326.70	664.35	664.39
247	984	Ac-F$r8AhYWEAc3cL$A-NH2	1328.72	665.36	665.43
248	985	Ac-F$r8AYWEAc3cL$Q-NH2	1371.72	686.86	1372.87
249	986	Ac-F$r8AYWEAibL$A-NH2	1316.72	659.36	1318.18
250	987	Ac-F$r8AYWEAL$A-NH2	1302.70	652.35	1303.75
251	988	Ac-LAF$r8AYWAAL$A-NH2	1428.82	715.41	715.49
252	989	Ac-LTF$r8HYWAAc3cL$S-NH2	1552.84	777.42	777.5
253	990	Ac-NleTF$r8HYWAQL$S-NH2	1597.87	799.94	800.04
254	991	Ac-VTF$r8HYWAQL$S-NH2	1583.85	792.93	793.04
255	992	Ac-FTF$r8HYWAQL$S-NH2	1631.85	816.93	817.02
256	993	Ac-WTF$r8HYWAQL$S-NH2	1670.86	836.43	836.85
257	994	Ac-RTF$r8HYWAQL$S-NH2	1640.88	821.44	821.9
258	995	Ac-KTF$r8HYWAQL$S-NH2	1612.88	807.44	807.91
259	996	Ac-LNleF$r8HYWAQL$S-NH2	1609.90	805.95	806.43
260	997	Ac-LVF$r8HYWAQL$S-NH2	1595.89	798.95	798.93
261	998	Ac-LFF$r8HYWAQL$S-NH2	1643.89	822.95	823.38
262	999	Ac-LWF$r8HYWAQL$S-NH2	1682.90	842.45	842.55
263	1000	Ac-LRF$r8HYWAQL$S-NH2	1652.92	827.46	827.52
264	1001	Ac-LKF$r8HYWAQL$S-NH2	1624.91	813.46	813.51
265	1002	Ac-LTF$r8NleYWAQL$S-NH2	1573.89	787.95	788.05
266	1003	Ac-LTF$r8VYWAQL$S-NH2	1559.88	780.94	780.98
267	1004	Ac-LTF$r8FYWAQL$S-NH2	1607.88	804.94	805.32
268	1005	Ac-LTF$r8WYWAQL$S-NH2	1646.89	824.45	824.86
269	1006	Ac-LTF$r8RYWAQL$S-NH2	1616.91	809.46	809.51
270	1007	Ac-LTF$r8KYWAQL$S-NH2	1588.90	795.45	795.48
271	1008	Ac-LTF$r8HNleWAQL$S-NH2	1547.89	774.95	774.98
272	1009	Ac-LTF$r8HVWAQL$S-NH2	1533.87	767.94	767.95
273	1010	Ac-LTF$r8HFWAQL$S-NH2	1581.87	791.94	792.3
274	1011	Ac-LTF$r8HWWAQL$S-NH2	1620.88	811.44	811.54
275	1012	Ac-LTF$r8HRWAQL$S-NH2	1590.90	796.45	796.52
276	1013	Ac-LTF$r8HKWAQL$S-NH2	1562.90	782.45	782.53
277	1014	Ac-LTF$r8HYWNleQL$S-NH2	1639.91	820.96	820.98
278	1015	Ac-LTF$r8HYWVQL$S-NH2	1625.90	813.95	814.03
279	1016	Ac-LTF$r8HYWFQL$S-NH2	1673.90	837.95	838.03
280	1017	Ac-LTF$r8HYWWQL$S-NH2	1712.91	857.46	857.5
281	1018	Ac-LTF$r8HYWKQL$S-NH2	1654.92	828.46	828.49
282	1019	Ac-LTF$r8HYWANleL$S-NH2	1582.89	792.45	792.52
283	1020	Ac-LTF$r8HYWAVL$S-NH2	1568.88	785.44	785.49
284	1021	Ac-LTF$r8HYWAFL$S-NH2	1616.88	809.44	809.47
285	1022	Ac-LTF$r8HYWAWL$S-NH2	1655.89	828.95	829
286	1023	Ac-LTF$r8HYWARL$S-NH2	1625.91	813.96	813.98
287	1024	Ac-LTF$r8HYWAQL$Nle-NH2	1623.92	812.96	813.39
288	1025	Ac-LTF$r8HYWAQL$V-NH2	1609.90	805.95	805.99
289	1026	Ac-LTF$r8HYWAQL$F-NH2	1657.90	829.95	830.26
290	1027	Ac-LTF$r8HYWAQL$W-NH2	1696.91	849.46	849.5
291	1028	Ac-LTF$r8HYWAQL$R-NH2	1666.94	834.47	834.56
292	1029	Ac-LTF$r8HYWAQL$K-NH2	1638.93	820.47	820.49
293	1030	Ac-Q$r8QQTFSN$WRLLAibQN-NH2	2080.12	1041.06	1041.54
294	1031	Ac-QSQQ$r8FSNLWR$LAibQN-NH2	2066.11	1034.06	1034.58
295	1032	Ac-LT2Pal$r8HYWAQL$S-NH2	1598.86	800.43	800.49
296	1033	Ac-LT3Pal$r8HYWAQL$S-NH2	1598.86	800.43	800.49
297	1034	Ac-LT4Pal$r8HYWAQL$S-NH2	1598.86	800.43	800.49
298	1035	Ac-LTF2CF3$r8HYWAQL$S-NH2	1665.85	833.93	834.01
299	1036	Ac-LTF2CN$r8HYWAQL$S-NH2	1622.86	812.43	812.47
300	1037	Ac-LTF2Me$r8HYWAQL$S-NH2	1611.88	806.94	807
301	1038	Ac-LTF3Cl$r8HYWAQL$S-NH2	1631.83	816.92	816.99
302	1039	Ac-LTF4CF3$r8HYWAQL$S-NH2	1665.85	833.93	833.94
303	1040	Ac-LTF4tBu$r8HYWAQL$S-NH2	1653.93	827.97	828.02
304	1041	Ac-LTF5F$r8HYWAQL$S-NH2	1687.82	844.91	844.96
305	1042	Ac-LTF$r8HY3BthAAQL$S-NH2	1614.83	808.42	808.48
306	1043	Ac-LTF2Br$r8HYWAQL$S-NH2	1675.78	838.89	838.97
307	1044	Ac-LTF4Br$r8HYWAQL$S-NH2	1675.78	838.89	839.86
308	1045	Ac-LTF2Cl$r8HYWAQL$S-NH2	1631.83	816.92	816.99
309	1046	Ac-LTF4Cl$r8HYWAQL$S-NH2	1631.83	816.92	817.36
310	1047	Ac-LTF3CN$r8HYWAQL$S-NH2	1622.86	812.43	812.47
311	1048	Ac-LTF4CN$r8HYWAQL$S-NH2	1622.86	812.43	812.47
312	1049	Ac-LTF34Cl2$r8HYWAQL$S-NH2	1665.79	833.90	833.94
313	1050	Ac-LTF34F2$r8HYWAQL$S-NH2	1633.85	817.93	817.95
314	1051	Ac-LTF35F2$r8HYWAQL$S-NH2	1633.85	817.93	817.95
315	1052	Ac-LTDip$r8HYWAQL$S-NH2	1673.90	837.95	838.01
316	1053	Ac-LTF2F$r8HYWAQL$S-NH2	1615.86	808.93	809
317	1054	Ac-LTF3F$r8HYWAQL$S-NH2	1615.86	808.93	809
318	1055	Ac-LTF4F$r8HYWAQL$S-NH2	1615.86	808.93	809
319	1056	Ac-LTF4I$r8HYWAQL$S-NH2	1723.76	862.88	862.94
320	1057	Ac-LTF3Me$r8HYWAQL$S-NH2	1611.88	806.94	807.07
321	1058	Ac-LTF4Me$r8HYWAQL$S-NH2	1611.88	806.94	807
322	1059	Ac-LT1Nal$r8HYWAQL$S-NH2	1647.88	824.94	824.98
323	1060	Ac-LT2Nal$r8HYWAQL$S-NH2	1647.88	824.94	825.06
324	1061	Ac-LTF3CF3$r8HYWAQL$S-NH2	1665.85	833.93	834.01
325	1062	Ac-LTF4NO2$r8HYWAQL$S-NH2	1642.85	822.43	822.46
326	1063	Ac-LTF3NO2$r8HYWAQL$S-NH2	1642.85	822.43	822.46
327	1064	Ac-LTF$r82ThiYWAQL$S-NH2	1613.83	807.92	807.96
328	1065	Ac-LTF$r8HBipWAQL$S-NH2	1657.90	829.95	830.01
329	1066	Ac-LTF$r8HF4tBuWAQL$S-NH2	1637.93	819.97	820.02
330	1067	Ac-LTF$r8HF4CF3WAQL$S-NH2	1649.86	825.93	826.02
331	1068	Ac-LTF$r8HF4ClWAQL$S-NH2	1615.83	808.92	809.37
332	1069	Ac-LTF$r8HF4MeWAQL$S-NH2	1595.89	798.95	799.01
333	1070	Ac-LTF$r8HF4BrWAQL$S-NH2	1659.78	830.89	830.98
334	1071	Ac-LTF$r8HF4CNWAQL$S-NH2	1606.87	804.44	804.56
335	1072	Ac-LTF$r8HF4NO2WAQL$S-NH2	1626.86	814.43	814.55
336	1073	Ac-LTF$r8H1NalWAQL$S-NH2	1631.89	816.95	817.06
337	1074	Ac-LTF$r8H2NalWAQL$S-NH2	1631.89	816.95	816.99
338	1075	Ac-LTF$r8HWAQL$S-NH2	1434.80	718.40	718.49
339	1076	Ac-LTF$r8HY1NalAQL$S-NH2	1608.87	805.44	805.52
340	1077	Ac-LTF$r8HY2NalAQL$S-NH2	1608.87	805.44	805.52
341	1078	Ac-LTF$r8HYWAQI$S-NH2	1597.87	799.94	800.07
342	1079	Ac-LTF$r8HYWAQNle$S-NH2	1597.87	799.94	800.44
343	1080	Ac-LTF$er8HYWAQL$eA-NH2	1581.87	791.94	791.98
344	1081	Ac-LTF$r8HYWAQL$Abu-NH2	1595.89	798.95	799.03
345	1082	Ac-LTF$r8HYWAbuQL$S-NH2	1611.88	806.94	804.47
346	1083	Ac-LAF$r8HYWAQL$S-NH2	1567.86	784.93	785.49
347	1084	Ac-LTF$r8NLWANleL$Q-NH2	1550.92	776.46	777.5
348	1085	Ac-LTF$r8ALWANleL$Q-NH2	1507.92	754.96	755.52
349	1086	Ac-LAF$r8NLWANleL$Q-NH2	1520.91	761.46	762.48
350	1087	Ac-F$r8AYWAAc3cL$A-NH2	1256.70	629.35	1257.56
351	1088	Ac-LTF$r8AYWAAL$S-NH2	1474.82	738.41	738.55
352	1089	Ac-LVF$r8AYWAQL$S-NH2	1529.87	765.94	766
353	1090	Ac-LTF$r8AYWAbuQL$S-NH2	1545.86	773.93	773.92
354	1091	Ac-LTF$r8AYWNleQL$S-NH2	1573.89	787.95	788.17
355	1092	Ac-LTF$r8AbuYWAQL$S-NH2	1545.86	773.93	773.99
356	1093	Ac-LTF$r8AYWHQL$S-NH2	1597.87	799.94	799.97
357	1094	Ac-LTF$r8AYWKQL$S-NH2	1588.90	795.45	795.53
358	1095	Ac-LTF$r8AYWOQL$S-NH2	1574.89	788.45	788.5
359	1096	Ac-LTF$r8AYWRQL$S-NH2	1616.91	809.46	809.51
360	1097	Ac-LTF$r8AYWSQL$S-NH2	1547.84	774.92	774.96
361	1098	Ac-LTF$r8AYWRAL$S-NH2	1559.89	780.95	780.95
362	1099	Ac-LTF$r8AYWRQL$A-NH2	1600.91	801.46	801.52
363	1100	Ac-LTF$r8AYWRAL$A-NH2	1543.89	772.95	773.03
364	1101	Ac-LTF$r5HYWAQL$s8S-NH2	1597.87	799.94	799.97
365	1102	Ac-LTF$HYWAQL$r8S-NH2	1597.87	799.94	799.97
366	1103	Ac-LTF$r8HYWAAL$S-NH2	1540.84	771.42	771.48
367	1104	Ac-LTF$r8HYWAAbuL$S-NH2	1554.86	778.43	778.51
368	1105	Ac-LTF$r8HYWALL$S-NH2	1582.89	792.45	792.49
369	1106	Ac-F$r8AYWHAL$A-NH2	1310.72	656.36	656.4
370	1107	Ac-F$r8AYWAAL$A-NH2	1244.70	623.35	1245.61
371	1108	Ac-F$r8AYWSAL$A-NH2	1260.69	631.35	1261.6
372	1109	Ac-F$r8AYWRAL$A-NH2	1329.76	665.88	1330.72
373	1110	Ac-F$r8AYWKAL$A-NH2	1301.75	651.88	1302.67
374	1111	Ac-F$r8AYWOAL$A-NH2	1287.74	644.87	1289.13
375	1112	Ac-F$r8VYWEAc3cL$A-NH2	1342.73	672.37	1343.67
376	1113	Ac-F$r8FYWEAc3cL$A-NH2	1390.73	696.37	1392.14
377	1114	Ac-F$r8WYWEAc3cL$A-NH2	1429.74	715.87	1431.44
378	1115	Ac-F$r8RYWEAc3cL$A-NH2	1399.77	700.89	700.95
379	1116	Ac-F$r8KYWEAc3cL$A-NH2	1371.76	686.88	686.97
380	1117	Ac-F$r8ANleWEAc3cL$A-NH2	1264.72	633.36	1265.59
381	1118	Ac-F$r8AVWEAc3cL$A-NH2	1250.71	626.36	1252.2
382	1119	Ac-F$r8AFWEAc3cL$A-NH2	1298.71	650.36	1299.64
383	1120	Ac-F$r8AWWEAc3cL$A-NH2	1337.72	669.86	1338.64
384	1121	Ac-F$r8ARWEAc3cL$A-NH2	1307.74	654.87	655
385	1122	Ac-F$r8AKWEAc3cL$A-NH2	1279.73	640.87	641.01
386	1123	Ac-F$r8AYWVAc3cL$A-NH2	1284.73	643.37	643.38
387	1124	Ac-F$r8AYWFAc3cL$A-NH2	1332.73	667.37	667.43
388	1125	Ac-F$r8AYWWAc3cL$A-NH2	1371.74	686.87	686.97
389	1126	Ac-F$r8AYWRAc3cL$A-NH2	1341.76	671.88	671.94
390	1127	Ac-F$r8AYWKAc3cL$A-NH2	1313.75	657.88	657.88
391	1128	Ac-F$r8AYWEVL$A-NH2	1330.73	666.37	666.47
392	1129	Ac-F$r8AYWEFL$A-NH2	1378.73	690.37	690.44
393	1130	Ac-F$r8AYWEWL$A-NH2	1417.74	709.87	709.91
394	1131	Ac-F$r8AYWERL$A-NH2	1387.77	694.89	1388.66
395	1132	Ac-F$r8AYWEKL$A-NH2	1359.76	680.88	1361.21
396	1133	Ac-F$r8AYWEAc3cL$V-NH2	1342.73	672.37	1343.59
397	1134	Ac-F$r8AYWEAc3cL$F-NH2	1390.73	696.37	1392.58
398	1135	Ac-F$r8AYWEAc3cL$W-NH2	1429.74	715.87	1431.29
399	1136	Ac-F$r8AYWEAc3cL$R-NH2	1399.77	700.89	700.95
400	1137	Ac-F$r8AYWEAc3cL$K-NH2	1371.76	686.88	686.97
401	1138	Ac-F$r8AYWEAc3cL$AV-NH2	1413.77	707.89	707.91
402	1139	Ac-F$r8AYWEAc3cL$AF-NH2	1461.77	731.89	731.96
403	1140	Ac-F$r8AYWEAc3cL$AW-NH2	1500.78	751.39	751.5
404	1141	Ac-F$r8AYWEAc3cL$AR-NH2	1470.80	736.40	736.47
405	1142	Ac-F$r8AYWEAc3cL$AK-NH2	1442.80	722.40	722.41
406	1143	Ac-F$r8AYWEAc3cL$AH-NH2	1451.76	726.88	726.93
407	1144	Ac-LTF2NO2$r8HYWAQL$S-NH2	1642.85	822.43	822.54
408	1145	Ac-LTA$r8HYAAQL$S-NH2	1406.79	704.40	704.5
409	1146	Ac-LTF$r8HYAAQL$S-NH2	1482.82	742.41	742.47
410	1147	Ac-QSQQTF$r8NLWALL$AN-NH2	1966.07	984.04	984.38
411	1148	Ac-QAibQQTF$r8NLWALL$AN-NH2	1964.09	983.05	983.42
412	1149	Ac-QAibQQTF$r8ALWALL$AN-NH2	1921.08	961.54	961.59
413	1150	Ac-AAAATF$r8AAWAAL$AA-NH2	1608.90	805.45	805.52
414	1151	Ac-F$r8AAWRAL$Q-NH2	1294.76	648.38	648.48
415	1152	Ac-TF$r8AAWAAL$Q-NH2	1310.74	656.37	1311.62
416	1153	Ac-TF$r8AAWRAL$A-NH2	1338.78	670.39	670.46
417	1154	Ac-VF$r8AAWRAL$Q-NH2	1393.82	697.91	697.99
418	1155	Ac-AF$r8AAWAAL$A-NH2	1223.71	612.86	1224.67
420	1156	Ac-TF$r8AAWKAL$Q-NH2	1367.80	684.90	684.97
421	1157	Ac-TF$r8AAWOAL$Q-NH2	1353.78	677.89	678.01
422	1158	Ac-TF$r8AAWSAL$Q-NH2	1326.73	664.37	664.47
423	1159	Ac-LTF$r8AAWRAL$Q-NH2	1508.89	755.45	755.49
424	1160	Ac-F$r8AYWAQL$A-NH2	1301.72	651.86	651.96
425	1161	Ac-F$r8AWWAAL$A-NH2	1267.71	634.86	634.87
426	1162	Ac-F$r8AWWAQL$A-NH2	1324.73	663.37	663.43
427	1163	Ac-F$r8AYWEAL$-NH2	1231.66	616.83	1232.93
428	1164	Ac-F$r8AYWAAL$-NH2	1173.66	587.83	1175.09
429	1165	Ac-F$r8AYWKAL$-NH2	1230.72	616.36	616.44
430	1166	Ac-F$r8AYWOAL$-NH2	1216.70	609.35	609.48
431	1167	Ac-F$r8AYWQAL$-NH2	1230.68	616.34	616.44
432	1168	Ac-F$r8AYWAQL$-NH2	1230.68	616.34	616.37
433	1169	Ac-F$r8HYWDQL$S-NH2	1427.72	714.86	714.86
434	1170	Ac-F$r8HFWEQL$S-NH2	1425.74	713.87	713.98
435	1171	Ac-F$r8AYWHQL$S-NH2	1383.73	692.87	692.96
436	1172	Ac-F$r8AYWKQL$S-NH2	1374.77	688.39	688.45
437	1173	Ac-F$r8AYWOQL$S-NH2	1360.75	681.38	681.49
438	1174	Ac-F$r8HYWSQL$S-NH2	1399.73	700.87	700.95
439	1175	Ac-F$r8HWWEQL$S-NH2	1464.76	733.38	733.44
440	1176	Ac-F$r8HWWAQL$S-NH2	1406.75	704.38	704.43
441	1177	Ac-F$r8AWWHQL$S-NH2	1406.75	704.38	704.43
442	1178	Ac-F$r8AWWKQL$S-NH2	1397.79	699.90	699.92
443	1179	Ac-F$r8AWWOQL$S-NH2	1383.77	692.89	692.96
444	1180	Ac-F$r8HWWSQL$S-NH2	1422.75	712.38	712.42
445	1181	Ac-LTF$r8NYWANleL$Q-NH2	1600.90	801.45	801.52
446	1182	Ac-LTF$r8NLWAQL$Q-NH2	1565.90	783.95	784.06
447	1183	Ac-LTF$r8NYWANleL$A-NH2	1543.88	772.94	773.03
448	1184	Ac-LTF$r8NLWAQL$A-NH2	1508.88	755.44	755.49
449	1185	Ac-LTF$r8AYWANleL$Q-NH2	1557.90	779.95	780.06
450	1186	Ac-LTF$r8ALWAQL$Q-NH2	1522.89	762.45	762.45
451	1187	Ac-LAF$r8NYWANleL$Q-NH2	1570.89	786.45	786.5
452	1188	Ac-LAF$r8NLWAQL$Q-NH2	1535.89	768.95	769.03
453	1189	Ac-LAF$r8AYWANleL$A-NH2	1470.86	736.43	736.47
454	1190	Ac-LAF$r8ALWAQL$A-NH2	1435.86	718.93	719.01
455	1191	Ac-LAF$r8AYWAAL$A-NH2	1428.82	715.41	715.41
456	1192	Ac-F$r8AYWEAc3cL$AAib-NH2	1399.75	700.88	700.95
457	1193	Ac-F$r8AYWAQL$AA-NH2	1372.75	687.38	687.78
458	1194	Ac-F$r8AYWAAc3cL$AA-NH2	1327.73	664.87	664.84
459	1195	Ac-F$r8AYWSAc3cL$AA-NH2	1343.73	672.87	672.9
460	1196	Ac-F$r8AYWEAc3cL$AS-NH2	1401.73	701.87	701.84
461	1197	Ac-F$r8AYWEAc3cL$AT-NH2	1415.75	708.88	708.87
462	1198	Ac-F$r8AYWEAc3cL$AL-NH2	1427.79	714.90	714.94
463	1199	Ac-F$r8AYWEAc3cL$AQ-NH2	1442.76	722.38	722.41
464	1200	Ac-F$r8AFWEAc3cL$AA-NH2	1369.74	685.87	685.93
465	1201	Ac-F$r8AWWEAc3cL$AA-NH2	1408.75	705.38	705.39
466	1202	Ac-F$r8AYWEAc3cL$SA-NH2	1401.73	701.87	701.99
467	1203	Ac-F$r8AYWEAL$AA-NH2	1373.74	687.87	687.93
468	1204	Ac-F$r8AYWENleL$AA-NH2	1415.79	708.90	708.94
469	1205	Ac-F$r8AYWEAc3cL$AbuA-NH2	1399.75	700.88	700.95
470	1206	Ac-F$r8AYWEAc3cL$NleA-NH2	1427.79	714.90	714.86
471	1207	Ac-F$r8AYWEAibL$NleA-NH2	1429.80	715.90	715.97
472	1208	Ac-F$r8AYWEAL$NleA-NH2	1415.79	708.90	708.94
473	1209	Ac-F$r8AYWENleL$NleA-NH2	1457.83	729.92	729.96
474	1210	Ac-F$r8AYWEAibL$Abu-NH2	1330.73	666.37	666.39
475	1211	Ac-F$r8AYWENleL$Abu-NH2	1358.76	680.38	680.39
476	1212	Ac-F$r8AYWEAL$Abu-NH2	1316.72	659.36	659.36
477	1213	Ac-LTF$r8AFWAQL$S-NH2	1515.85	758.93	759.12
478	1214	Ac-LTF$r8AWWAQL$S-NH2	1554.86	778.43	778.51
479	1215	Ac-LTF$r8AYWAQI$S-NH2	1531.84	766.92	766.96
480	1216	Ac-LTF$r8AYWAQNle$S-NH2	1531.84	766.92	766.96
481	1217	Ac-LTF$r8AYWAQL$SA-NH2	1602.88	802.44	802.48
482	1218	Ac-LTF$r8AWWAQL$A-NH2	1538.87	770.44	770.89
483	1219	Ac-LTF$r8AYWAQI$A-NH2	1515.85	758.93	759.42
484	1220	Ac-LTF$r8AYWAQNle$A-NH2	1515.85	758.93	759.42
485	1221	Ac-LTF$r8AYWAQL$AA-NH2	1586.89	794.45	794.94
486	1222	Ac-LTF$r8HWWAQL$S-NH2	1620.88	811.44	811.47
487	1223	Ac-LTF$r8HRWAQL$S-NH2	1590.90	796.45	796.52
488	1224	Ac-LTF$r8HKWAQL$S-NH2	1562.90	782.45	782.53
489	1225	Ac-LTF$r8HYWAQL$W-NH2	1696.91	849.46	849.5
491	1226	Ac-F$r8AYWAbuAL$A-NH2	1258.71	630.36	630.5
492	1227	Ac-F$r8AbuYWEAL$A-NH2	1316.72	659.36	659.51
493	1228	Ac-NlePRF%r8NYWRLL%QN-NH2	1954.13	978.07	978.54
494	1229	Ac-TSF%r8HYWAQL%S-NH2	1573.83	787.92	787.98
495	1230	Ac-LTF%r8AYWAQL%S-NH2	1533.86	767.93	768
496	1231	Ac-HTF$r8HYWAQL$S-NH2	1621.84	811.92	811.96
497	1232	Ac-LHF$r8HYWAQL$S-NH2	1633.88	817.94	818.02
498	1233	Ac-LTF$r8HHWAQL$S-NH2	1571.86	786.93	786.94
499	1234	Ac-LTF$r8HYWHQL$S-NH2	1663.89	832.95	832.38
500	1235	Ac-LTF$r8HYWAHL$S-NH2	1606.87	804.44	804.48
501	1236	Ac-LTF$r8HYWAQL$H-NH2	1647.89	824.95	824.98
502	1237	Ac-LTF$r8HYWAQL$S-NHPr	1639.91	820.96	820.98
503	1238	Ac-LTF$r8HYWAQL$S-NHsBu	1653.93	827.97	828.02
504	1239	Ac-LTF$r8HYWAQL$S-NHiBu	1653.93	827.97	828.02
505	1240	Ac-LTF$r8HYWAQL$S-NHBn	1687.91	844.96	844.44
506	1241	Ac-LTF$r8HYWAQL$S-NHPe	1700.92	851.46	851.99
507	1242	Ac-LTF$r8HYWAQL$S-NHChx	1679.94	840.97	841.04
508	1243	Ac-ETF$r8AYWAQL$S-NH2	1547.80	774.90	774.96
509	1244	Ac-STF$r8AYWAQL$S-NH2	1505.79	753.90	753.94
510	1245	Ac-LEF$r8AYWAQL$S-NH2	1559.84	780.92	781.25
511	1246	Ac-LSF$r8AYWAQL$S-NH2	1517.83	759.92	759.93
512	1247	Ac-LTF$r8EYWAQL$S-NH2	1589.85	795.93	795.97
513	1248	Ac-LTF$r8SYWAQL$S-NH2	1547.84	774.92	774.96
514	1249	Ac-LTF$r8AYWEQL$S-NH2	1589.85	795.93	795.9
515	1250	Ac-LTF$r8AYWAEL$S-NH2	1532.83	767.42	766.96
516	1251	Ac-LTF$r8AYWASL$S-NH2	1490.82	746.41	746.46
517	1252	Ac-LTF$r8AYWAQL$E-NH2	1573.85	787.93	787.98
518	1253	Ac-LTF2CN$r8HYWAQL$S-NH2	1622.86	812.43	812.47
519	1254	Ac-LTF3Cl$r8HYWAQL$S-NH2	1631.83	816.92	816.99
520	1255	Ac-LTDip$r8HYWAQL$S-NH2	1673.90	837.95	838.01
521	1256	Ac-LTF$r8HYWAQTle$S-NH2	1597.87	799.94	800.04
522	1257	Ac-F$r8AY6clWEAL$A-NH2	1336.66	669.33	1338.56
523	1258	Ac-F$r8AYdl6brWEAL$A-NH2	1380.61	691.31	692.2
524	1259	Ac-F$r8AYdl6fWEAL$A-NH2	1320.69	661.35	1321.61
525	1260	Ac-F$r8AYdl4mWEAL$A-NH2	1316.72	659.36	659.36
526	1261	Ac-F$r8AYdl5clWEAL$A-NH2	1336.66	669.33	669.35
527	1262	Ac-F$r8AYdl7mWEAL$A-NH2	1316.72	659.36	659.36
528	1263	Ac-LTF%r8HYWAQL%A-NH2	1583.89	792.95	793.01
529	1264	Ac-LTF$r8HCouWAQL$S-NH2	1679.87	840.94	841.38
530	1265	Ac-LTFEHCouWAQLTS-NH2	1617.75	809.88	809.96
531	1266	Ac-LTA$r8HCouWAQL$S-NH2	1603.84	802.92	803.36
532	1267	Ac-F$r8AYWEAL$AbuA-NH2	1387.75	694.88	694.88
533	1268	Ac-F$r8AYWEAISAA-NH2	1373.74	687.87	687.93
534	1269	Ac-F$r8AYWEANle$AA-NH2	1373.74	687.87	687.93
535	1270	Ac-F$r8AYWEAmlL$AA-NH2	1429.80	715.90	715.97
536	1271	Ac-F$r8AYWQAL$AA-NH2	1372.75	687.38	687.48
537	1272	Ac-F$r8AYWAAL$AA-NH2	1315.73	658.87	658.92
538	1273	Ac-F$r8AYWAbuAL$AA-NH2	1329.75	665.88	665.95
539	1274	Ac-F$r8AYWNleAL$AA-NH2	1357.78	679.89	679.94
540	1275	Ac-F$r8AbuYWEAL$AA-NH2	1387.75	694.88	694.96
541	1276	Ac-F$r8NleYWEAL$AA-NH2	1415.79	708.90	708.94
542	1277	Ac-F$r8FYWEAL$AA-NH2	1449.77	725.89	725.97
543	1278	Ac-LTF$r8HYWAQhL$S-NH2	1611.88	806.94	807
544	1279	Ac-LTF$r8HYWAQAdm$S-NH2	1675.91	838.96	839.04
545	1280	Ac-LTF$r8HYWAQIgl$S-NH2	1659.88	830.94	829.94
546	1281	Ac-F$r8AYWAQL$AA-NH2	1372.75	687.38	687.48
547	1282	Ac-LTF$r8ALWAQL$Q-NH2	1522.89	762.45	762.52
548	1283	Ac-F$r8AYWEAL$AA-NH2	1373.74	687.87	687.93
549	1284	Ac-F$r8AYWENleL$AA-NH2	1415.79	708.90	708.94
550	1285	Ac-F$r8AYWEAibL$Abu-NH2	1330.73	666.37	666.39
551	1286	Ac-F$r8AYWENleL$Abu-NH2	1358.76	680.38	680.38
552	1287	Ac-F$r8AYWEAL$Abu-NH2	1316.72	659.36	659.36
553	1288	Ac-F$r8AYWEAc3cL$AbuA-NH2	1399.75	700.88	700.95
554	1289	Ac-F$r8AYWEAc3cL$NleA-NH2	1427.79	714.90	715.01
555	1290	H-LTF$r8AYWAQL$S-NH2	1489.83	745.92	745.95
556	1291	mdPEG3-LTF$r8AYWAQL$S-NH2	1679.92	840.96	840.97
557	1292	mdPEG7-LTF$r8AYWAQL$S-NH2	1856.02	929.01	929.03
558	1293	Ac-F$r8ApmpEt6clWEAL$A-NH2	1470.71	736.36	788.17
559	1294	Ac-LTF3Cl$r8AYWAQL$S-NH2	1565.81	783.91	809.18
560	1295	Ac-LTF3Cl$r8HYWAQL$A-NH2	1615.83	808.92	875.24
561	1296	Ac-LTF3Cl$r8HYWWQL$S-NH2	1746.87	874.44	841.65
562	1297	Ac-LTF3Cl$r8AYWWQL$S-NH2	1680.85	841.43	824.63
563	1298	Ac-LTF$r8AYWWQL$S-NH2	1646.89	824.45	849.98
564	1299	Ac-LTF$r8HYWWQL$A-NH2	1696.91	849.46	816.67
565	1300	Ac-LTF$r8AYWWQL$A-NH2	1630.89	816.45	776.15
566	1301	Ac-LTF4F$r8AYWAQL$S-NH2	1549.83	775.92	776.15
567	1302	Ac-LTF2F$r8AYWAQL$S-NH2	1549.83	775.92	776.15
568	1303	Ac-LTF3F$r8AYWAQL$S-NH2	1549.83	775.92	785.12
569	1304	Ac-LTF34F2$r8AYWAQL$S-NH2	1567.83	784.92	785.12
570	1305	Ac-LTF35F2$r8AYWAQL$S-NH2	1567.83	784.92	1338.74
571	1306	Ac-F3Cl$r8AYWEAL$A-NH2	1336.66	669.33	705.28
572	1307	Ac-F3Cl$r8AYWEAL$AA-NH2	1407.70	704.85	680.11
573	1308	Ac-F$r8AY6clWEAL$AA-NH2	1407.70	704.85	736.83
574	1309	Ac-F$r8AY6clWEAL$-NH2	1265.63	633.82	784.1
575	1310	Ac-LTF$r8HYWAQLSt/S-NH2	16.03	9.02	826.98
576	1311	Ac-LTF$r8HYWAQL$S-NHsBu	1653.93	827.97	828.02
577	1312	Ac-STF$r8AYWAQL$S-NH2	1505.79	753.90	753.94
578	1313	Ac-LTF$r8AYWAEL$S-NH2	1532.83	767.42	767.41
579	1314	Ac-LTF$r8AYWAQL$E-NH2	1573.85	787.93	787.98
580	1315	mdPEG3-LTF$r8AYWAQL$S-NH2	1679.92	840.96	840.97
581	1316	Ac-LTF$r8AYWAQhL$S-NH2	1545.86	773.93	774.31
583	1317	Ac-LTF$r8AYWAQCha$S-NH2	1571.88	786.94	787.3
584	1318	Ac-LTF$r8AYWAQChg$S-NH2	1557.86	779.93	780.4
585	1319	Ac-LTF$r8AYWAQCba$S-NH2	1543.84	772.92	780.13
586	1320	Ac-LTF$r8AYWAQF$S-NH2	1565.83	783.92	784.2
587	1321	Ac-LTF4F$r8HYWAQhL$S-NH2	1629.87	815.94	815.36
588	1322	Ac-LTF4F$r8HYWAQCha$S-NH2	1655.89	828.95	828.39
589	1323	Ac-LTF4F$r8HYWAQChg$S-NH2	1641.87	821.94	821.35
590	1324	Ac-LTF4F$r8HYWAQCba$S-NH2	1627.86	814.93	814.32
591	1325	Ac-LTF4F$r8AYWAQhL$S-NH2	1563.85	782.93	782.36
592	1326	Ac-LTF4F$r8AYWAQCha$S-NH2	1589.87	795.94	795.38
593	1327	Ac-LTF4F$r8AYWAQChg$S-NH2	1575.85	788.93	788.35
594	1328	Ac-LTF4F$r8AYWAQCba$S-NH2	1561.83	781.92	781.39
595	1329	Ac-LTF3Cl$r8AYWAQhL$S-NH2	1579.82	790.91	790.35
596	1330	Ac-LTF3Cl$r8AYWAQCha$S-NH2	1605.84	803.92	803.67
597	1331	Ac-LTF3Cl$r8AYWAQChg$S-NH2	1591.82	796.91	796.34
598	1332	Ac-LTF3Cl$r8AYWAQCba$S-NH2	1577.81	789.91	789.39
599	1333	Ac-LTF$r8AYWAQhF$S-NH2	1579.84	790.92	791.14
600	1334	Ac-LTF$r8AYWAQF3CF3$S-NH2	1633.82	817.91	818.15
601	1335	Ac-LTF$r8AYWAQF3Me$S-NH2	1581.86	791.93	791.32
602	1336	Ac-LTF$r8AYWAQ1Nal$S-NH2	1615.84	808.92	809.18
603	1337	Ac-LTF$r8AYWAQBip$S-NH2	1641.86	821.93	822.13
604	1338	Ac-LTF$r8FYWAQL$A-NH2	1591.88	796.94	797.33
605	1339	Ac-LTF$r8HYWAQL$S-NHAm	1667.94	834.97	835.92
606	1340	Ac-LTF$r8HYWAQL$S-NHiAm	1667.94	834.97	835.55
607	1341	Ac-LTF$r8HYWAQL$S-NHnPr3Ph	1715.94	858.97	859.79
608	1342	Ac-LTF$r8HYWAQL$S-NHnBu3,3Me	1681.96	841.98	842.49
610	1343	Ac-LTF$r8HYWAQL$S-NHnPr	1639.91	820.96	821.58
611	1344	Ac-LTF$r8HYWAQL$S-NHnEt2Ch	1707.98	854.99	855.35
612	1345	Ac-LTF$r8HYWAQL$S-NHHex	1681.96	841.98	842.4
613	1346	Ac-LTF$r8AYWAQL$S-NHmdPeg2	1633.91	817.96	818.35
614	1347	Ac-LTF$r8AYWAQL$A-NHmdPeg2	1617.92	809.96	810.3
615	1348	Ac-LTF$r8AYWAQL$A-NHmdPeg4	1705.97	853.99	854.33
616	1349	Ac-F$r8AYdl4mWEAL$A-NH2	1316.72	659.36	659.44
617	1350	Ac-F$r8AYdl5clWEAL$A-NH2	1336.66	669.33	669.43
618	1351	Ac-LThF$r8AYWAQL$S-NH2	1545.86	773.93	774.11
619	1352	Ac-LT2Nal$r8AYWAQL$S-NH2	1581.86	791.93	792.43
620	1353	Ac-LTA$r8AYWAQL$S-NH2	1455.81	728.91	729.15
621	1354	Ac-LTF$r8AYWVQL$S-NH2	1559.88	780.94	781.24
622	1355	Ac-LTF$r8HYWAAL$A-NH2	1524.85	763.43	763.86
623	1356	Ac-LTF$r8VYWAQL$A-NH2	1543.88	772.94	773.37
624	1357	Ac-LTF$r8IYWAQL$S-NH2	1573.89	787.95	788.17
625	1358	Ac-FTF$r8VYWSQL$S-NH2	1609.85	805.93	806.22
626	1359	Ac-ITF$r8FYWAQL$S-NH2	1607.88	804.94	805.2
627	1360	Ac-2NalTF$r8VYWSQL$S-NH2	1659.87	830.94	831.2
628	1361	Ac-ITF$r8LYWSQL$S-NH2	1589.89	795.95	796.13
629	1362	Ac-FTF$r8FYWAQL$S-NH2	1641.86	821.93	822.13
630	1363	Ac-WTF$r8VYWAQL$S-NH2	1632.87	817.44	817.69
631	1364	Ac-WTF$r8WYWAQL$S-NH2	1719.88	860.94	861.36
632	1365	Ac-VTF$r8AYWSQL$S-NH2	1533.82	767.91	768.19
633	1366	Ac-WTF$r8FYWSQL$S-NH2	1696.87	849.44	849.7
634	1367	Ac-FTF$r8IYWAQL$S-NH2	1607.88	804.94	805.2
635	1368	Ac-WTF$r8VYWSQL$S-NH2	1648.87	825.44	824.8
636	1369	Ac-FTF$r8LYWSQL$S-NH2	1623.87	812.94	812.8
637	1370	Ac-YTF$r8FYWSQL$S-NH2	1673.85	837.93	837.8
638	1371	Ac-LTF$r8AY6clWEAL$A-NH2	1550.79	776.40	776.14
639	1372	Ac-LTF$r8AY6clWSQL$S-NH2	1581.80	791.90	791.68
640	1373	Ac-F$r8AY6clWSAL$A-NH2	1294.65	648.33	647.67
641	1374	Ac-F$r8AY6clWQAL$AA-NH2	1406.72	704.36	703.84
642	1375	Ac-LHF$r8AYWAQL$S-NH2	1567.86	784.93	785.21
643	1376	Ac-LTF$r8AYWAQL$S-NH2	1531.84	766.92	767.17
644	1377	Ac-LTF$r8AHWAQL$S-NH2	1505.84	753.92	754.13
645	1378	Ac-LTF$r8AYWAHL$S-NH2	1540.84	771.42	771.61
646	1379	Ac-LTF$r8AYWAQL$H-NH2	1581.87	791.94	792.15
647	1380	H-LTF$r8AYWAQL$A-NH2	1473.84	737.92	737.29
648	1381	Ac-HHF$r8AYWAQL$S-NH2	1591.83	796.92	797.35
649	1382	Ac-aAibWTF$r8VYWSQL$S-NH2	1804.96	903.48	903.64
650	1383	Ac-AibWTF$r8HYWAQL$S-NH2	1755.91	878.96	879.4
651	1384	Ac-AibAWTF$r8HYWAQL$S-NH2	1826.95	914.48	914.7
652	1385	Ac-fWTF$r8HYWAQL$S-NH2	1817.93	909.97	910.1
653	1386	Ac-AibWWTF$r8HYWAQL$S-NH2	1941.99	972.00	972.2
654	1387	Ac-WTF$r8LYWSQL$S-NH2	1662.88	832.44	832.8
655	1388	Ac-WTF$r8NleYWSQL$S-NH2	1662.88	832.44	832.6
656	1389	Ac-LTF$r8AYWSQL$a-NH2	1531.84	766.92	767.2
657	1390	Ac-LTF$r8EYWARL$A-NH2	1601.90	801.95	802.1
658	1391	Ac-LTF$r8EYWAHL$A-NH2	1582.86	792.43	792.6
659	1392	Ac-aTF$r8AYWAQL$S-NH2	1489.80	745.90	746.08
660	1393	Ac-AibTF$r8AYWAQL$S-NH2	1503.81	752.91	753.11
661	1394	Ac-AmfTF$r8AYWAQL$S-NH2	1579.84	790.92	791.14
662	1395	Ac-AmwTF$r8AYWAQL$S-NH2	1618.86	810.43	810.66
663	1396	Ac-NmLTF$r8AYWAQL$S-NH2	1545.86	773.93	774.11
664	1397	Ac-LNmTF$r8AYWAQL$S-NH2	1545.86	773.93	774.11
665	1398	Ac-LSarF$r8AYWAQL$S-NH2	1501.83	751.92	752.18
667	1399	Ac-LGF$r8AYWAQL$S-NH2	1487.82	744.91	745.15
668	1400	Ac-LTNmF$r8AYWAQL$S-NH2	1545.86	773.93	774.2
669	1401	Ac-TF$r8AYWAQL$S-NH2	1418.76	710.38	710.64
670	1402	Ac-ETF$r8AYWAQL$A-NH2	1531.81	766.91	767.2
671	1403	Ac-LTF$r8EYWAQL$A-NH2	1573.85	787.93	788.1
672	1404	Ac-LT2Nal$r8AYWSQL$S-NH2	1597.85	799.93	800.4
673	1405	Ac-LTF$r8AYWAAL$S-NH2	1474.82	738.41	738.68
674	1406	Ac-LTF$r8AYWAQhCha$S-NH2	1585.89	793.95	794.19
675	1407	Ac-LTF$r8AYWAQChg$S-NH2	1557.86	779.93	780.97
676	1408	Ac-LTF$r8AYWAQCba$S-NH2	1543.84	772.92	773.19
677	1409	Ac-LTF$r8AYWAQF3CF3$S-NH2	1633.82	817.91	818.15
678	1410	Ac-LTF$r8AYWAQ1Nal$S-NH2	1615.84	808.92	809.18
679	1411	Ac-LTF$r8AYWAQBip$S-NH2	1641.86	821.93	822.32
680	1412	Ac-LT2Nal$r8AYWAQL$S-NH2	1581.86	791.93	792.15
681	1413	Ac-LTF$r8AYWVQL$S-NH2	1559.88	780.94	781.62
682	1414	Ac-LTF$r8AWWAQL$S-NH2	1554.86	778.43	778.65
683	1415	Ac-FTF$r8VYWSQL$S-NH2	1609.85	805.93	806.12
684	1416	Ac-ITF$r8FYWAQL$S-NH2	1607.88	804.94	805.2
685	1417	Ac-ITF$r8LYWSQL$S-NH2	1589.89	795.95	796.22
686	1418	Ac-FTF$r8FYWAQL$S-NH2	1641.86	821.93	822.41
687	1419	Ac-VTF$r8AYWSQL$S-NH2	1533.82	767.91	768.19
688	1420	Ac-LTF$r8AHWAQL$S-NH2	1505.84	753.92	754.31
689	1421	Ac-LTF$r8AYWAQL$H-NH2	1581.87	791.94	791.94
690	1422	Ac-LTF$r8AYWAHL$S-NH2	1540.84	771.42	771.61
691	1423	Ac-aAibWTF$r8VYWSQL$S-NH2	1804.96	903.48	903.9
692	1424	Ac-AibWTF$r8HYWAQL$S-NH2	1755.91	878.96	879.5
693	1425	Ac-AibAWTF$r8HYWAQL$S-NH2	1826.95	914.48	914.7
694	1426	Ac-fWTF$r8HYWAQL$S-NH2	1817.93	909.97	910.2
695	1427	Ac-AibWWTF$r8HYWAQL$S-NH2	1941.99	972.00	972.7
696	1428	Ac-WTF$r8LYWSQL$S-NH2	1662.88	832.44	832.7
697	1429	Ac-WTF$r8NleYWSQL$S-NH2	1662.88	832.44	832.7
698	1430	Ac-LTF$r8AYWSQL$a-NH2	1531.84	766.92	767.2
699	1431	Ac-LTF$r8EYWARL$A-NH2	1601.90	801.95	802.2
700	1432	Ac-LTF$r8EYWAHL$A-NH2	1582.86	792.43	792.6
701	1433	Ac-aTF$r8AYWAQL$S-NH2	1489.80	745.90	746.1
702	1434	Ac-AibTF$r8AYWAQL$S-NH2	1503.81	752.91	753.2
703	1435	Ac-AmfTF$r8AYWAQL$S-NH2	1579.84	790.92	791.2
704	1436	Ac-AmwTF$r8AYWAQL$S-NH2	1618.86	810.43	810.7
705	1437	Ac-NmLTF$r8AYWAQL$S-NH2	1545.86	773.93	774.1
706	1438	Ac-LNmTF$r8AYWAQL$S-NH2	1545.86	773.93	774.4
707	1439	Ac-LSarF$r8AYWAQL$S-NH2	1501.83	751.92	752.1
708	1440	Ac-TF$r8AYWAQL$S-NH2	1418.76	710.38	710.8
709	1441	Ac-ETF$r8AYWAQL$A-NH2	1531.81	766.91	767.4
710	1442	Ac-LTF$r8EYWAQL$A-NH2	1573.85	787.93	788.2
711	1443	Ac-WTF$r8VYWSQL$S-NH2	1648.87	825.44	825.2
713	1444	Ac-YTF$r8FYWSQL$S-NH2	1673.85	837.93	837.3
714	1445	Ac-F$r8AY6clWSAL$A-NH2	1294.65	648.33	647.74
715	1446	Ac-ETF$r8EYWVQL$S-NH2	1633.84	817.92	817.36
716	1447	Ac-ETF$r8EHWAQL$A-NH2	1563.81	782.91	782.36
717	1448	Ac-ITF$r8EYWAQL$S-NH2	1589.85	795.93	795.38
718	1449	Ac-ITF$r8EHWVQL$A-NH2	1575.88	788.94	788.42
719	1450	Ac-ITF$r8EHWAQL$S-NH2	1563.85	782.93	782.43
720	1451	Ac-LTF4F$r8AYWAQCba$S-NH2	1561.83	781.92	781.32
721	1452	Ac-LTF3Cl$r8AYWAQhL$S-NH2	1579.82	790.91	790.64
722	1453	Ac-LTF3Cl$r8AYWAQCha$S-NH2	1605.84	803.92	803.37
723	1454	Ac-LTF3Cl$r8AYWAQChg$S-NH2	1591.82	796.91	796.27
724	1455	Ac-LTF3Cl$r8AYWAQCba$S-NH2	1577.81	789.91	789.83
725	1456	Ac-LTF$r8AY6clWSQL$S-NH2	1581.80	791.90	791.75
726	1457	Ac-LTF4F$r8HYWAQhL$S-NH2	1629.87	815.94	815.36
727	1458	Ac-LTF4F$r8HYWAQCba$S-NH2	1627.86	814.93	814.32
728	1459	Ac-LTF4F$r8AYWAQhL$S-NH2	1563.85	782.93	782.36
729	1460	Ac-LTF4F$r8AYWAQChg$S-NH2	1575.85	788.93	788.35
730	1461	Ac-ETF$r8EYWVAL$S-NH2	1576.82	789.41	788.79
731	1462	Ac-ETF$r8EHWAAL$A-NH2	1506.79	754.40	754.8
732	1463	Ac-ITF$r8EYWAAL$S-NH2	1532.83	767.42	767.75
733	1464	Ac-ITF$r8EHWVAL$A-NH2	1518.86	760.43	760.81
734	1465	Ac-ITF$r8EHWAAL$S-NH2	1506.82	754.41	754.8
735	1466	Pam-LTF$r8EYWAQL$S-NH2	1786.07	894.04	894.48
736	1467	Pam-ETF$r8EYWAQL$S-NH2	1802.03	902.02	902.34
737	1468	Ac-LTF$r8AYWLQL$S-NH2	1573.89	787.95	787.39
738	1469	Ac-LTF$r8EYWLQL$S-NH2	1631.90	816.95	817.33
739	1470	Ac-LTF$r8EHWLQL$S-NH2	1605.89	803.95	804.29
740	1471	Ac-LTF$r8VYWAQL$S-NH2	1559.88	780.94	781.34
741	1472	Ac-LTF$r8AYWSQL$S-NH2	1547.84	774.92	775.33
742	1473	Ac-ETF$r8AYWAQL$S-NH2	1547.80	774.90	775.7
743	1474	Ac-LTF$r8EYWAQL$S-NH2	1589.85	795.93	796.33
744	1475	Ac-LTF$r8HYWAQL$S-NHAm	1667.94	834.97	835.37
745	1476	Ac-LTF$r8HYWAQL$S-NHiAm	1667.94	834.97	835.27
746	1477	Ac-LTF$r8HYWAQL$S-NHnPr3Ph	1715.94	858.97	859.42
747	1478	Ac-LTF$r8HYWAQL$S-NHnBu3,3Me	1681.96	841.98	842.67
748	1479	Ac-LTF$r8HYWAQL$S-NHnBu	1653.93	827.97	828.24
749	1480	Ac-LTF$r8HYWAQL$S-NHnPr	1639.91	820.96	821.31
750	1481	Ac-LTF$r8HYWAQL$S-NHnEt2Ch	1707.98	854.99	855.35
751	1482	Ac-LTF$r8HYWAQL$S-NHHex	1681.96	841.98	842.4
752	1483	Ac-LTF$r8AYWAQL$S-NHmdPeg2	1633.91	817.96	855.35
753	1484	Ac-LTF$r8AYWAQL$A-NHmdPeg2	1617.92	809.96	810.58
754	1485	Ac-LTF$r5AYWAAL$s8S-NH2	1474.82	738.41	738.79
755	1486	Ac-LTF$r8AYWCouQL$S-NH2	1705.88	853.94	854.61
756	1487	Ac-LTF$r8CouYWAQL$S-NH2	1705.88	853.94	854.7
757	1488	Ac-CouTF$r8AYWAQL$S-NH2	1663.83	832.92	833.33
758	1489	H-LTF$r8AYWAQL$A-NH2	1473.84	737.92	737.29
759	1490	Ac-HHF$r8AYWAQL$S-NH2	1591.83	796.92	797.72
760	1491	Ac-LT2Nal$r8AYWSQL$S-NH2	1597.85	799.93	800.68
761	1492	Ac-LTF$r8HCouWAQL$S-NH2	1679.87	840.94	841.38
762	1493	Ac-LTF$r8AYWCou2QL$S-NH2	1789.94	895.97	896.51
763	1494	Ac-LTF$r8Cou2YWAQL$S-NH2	1789.94	895.97	896.5
764	1495	Ac-Cou2TF$r8AYWAQL$S-NH2	1747.90	874.95	875.42
765	1496	Ac-LTF$r8ACou2WAQL$S-NH2	1697.92	849.96	850.82
766	1497	Dmaac-LTF$r8AYWAQL$S-NH2	1574.89	788.45	788.82
767	1498	Hexac-LTF$r8AYWAQL$S-NH2	1587.91	794.96	795.11
768	1499	Napac-LTF$r8AYWAQL$S-NH2	1657.89	829.95	830.36
769	1500	Pam-LTF$r8AYWAQL$S-NH2	1728.06	865.03	865.45
770	1501	Ac-LT2Nal$r8HYAAQL$S-NH2	1532.84	767.42	767.61
771	1502	Ac-LT2Nal$/r8HYWAQLS/S-NH2	1675.91	838.96	839.1
772	1503	Ac-LT2Nal$r8HYFAQL$S-NH2	1608.87	805.44	805.9
773	1504	Ac-LT2Nal$r8HWAAQL$S-NH2	1555.86	778.93	779.08
774	1505	Ac-LT2Nal$r8HYAWQL$S-NH2	1647.88	824.94	825.04
775	1506	Ac-LT2Nal$r8HYAAQW$S-NH2	1605.83	803.92	804.05
776	1507	Ac-LTW$r8HYWAQL$S-NH2	1636.88	819.44	819.95
777	1508	Ac-LT1Nal$r8HYWAQL$S-NH2	1647.88	824.94	825.41

In some embodiments, the peptidomimetic macrocycles disclosed herein do not comprise a peptidomimetic macrocycle structure as shown in Table 2b.
Table 2c shows examples of non-crosslinked polypeptides comprising D-amino acids.

TABLE 2c

	SEQ
	ID			Exact	Found	Calc	Calc	Calc
SP	NO:	Sequence	Isomer	Mass	Mass	(M + 1)/1	(M + 2)/2	(M + 3)/3

SP778	1509	Ac-tawyanfekllr-NH2			777.46
SP779	1510	Ac-tawyanf4CF3ekllr-NH2			811.41

Peptidomimetic macrocycles can also be prepared that target or interact with proteins that a virus needs for infection or replication within a host cell. Such viruses can be, for example, influenza viruses belonging to Orthomyxoviridae family of viruses. This family also includes Thogoto viruses and Dhoriviruses. There are several types and subtypes of influenza viruses known, which infect humans and other species. Influenza type A viruses infect people, birds, pigs, horses, seals and other animals, but wild birds are the natural hosts for these viruses. Influenza type A viruses are divided into subtypes and named on the basis of two proteins on the surface of the virus: hemagglutinin (HA) and neuraminidase (NA). For example, an “H7N2 virus” designates an influenza A subtype that has an HA7 protein and an NA2 protein. Similarly an “H5N1” virus has an HA 5 protein and an NA1 protein. There are 16 known HA subtypes and 9 known NA subtypes. Many different combinations of HA and NA proteins are possible. Only some influenza A subtypes (i.e., H1N1, H1N2, and H3N2) are currently in general circulation among people. Other subtypes are found most commonly in other animal species. For example, H7N7 and H3N8 viruses cause illness in horses, and H3N8 also has recently been shown to cause illness in dogs.
Antiviral agents according to the invention can be used to protect high-risk groups (hospital units, institutes caring for elderly, immuno-suppressed individuals), and on a case by case basis. A potential use for antiviral agents is to limit the spread and severity of the future pandemics whether caused by avian H5N1 or other strains of influenza virus. Avian influenza A viruses of the subtypes H5 and H7, including H5N1, H7N7, and H7N3 viruses, have been associated with high pathogenicity, and human infection with these viruses have ranged from mild (H7N3, H7N7) to severe and fatal disease (H7N7, H5N1). Human illness due to infection with low pathogenicity viruses has been documented, including very mild symptoms (e.g., conjunctivitis) to influenza-like illness. Examples of low pathogenicity viruses that have infected humans include H7N7, H9N2, and H7N2.
Influenza B viruses are usually found in humans but can also infect seals. Unlike influenza A viruses, these viruses are not classified according to subtype. Influenza B viruses can cause morbidity and mortality among humans, but in general are associated with less severe epidemics than influenza A viruses. Although influenza type B viruses can cause human epidemics, they have not caused pandemics.
Influenza type C viruses cause mild illness in humans and do not cause epidemics or pandemics. These viruses can also infect dogs and pigs. These viruses are not classified according to subtype.
Influenza viruses differ from each other in respect to cell surface receptor specificity and cell tropism, however they use common entry pathways. Charting these pathways and identification of host cell proteins involved in virus influenza transmission, entry, replication, biosynthesis, assembly, or exit allows the development of general agents against existing and emerging strains of influenza. The agents can also prove useful against unrelated viruses that use similar pathways. For example, the agents can protect airway epithelial cells against a number of different viruses in addition to influenza viruses.
In one embodiment the targeted virus is an adenovirus. Adenoviruses most commonly cause respiratory illness; symptoms of respiratory illness caused by adenovirus infection range from the common cold syndrome to pneumonia, croup, and bronchitis. Patients with compromised immune systems are especially susceptible to severe complications of adenovirus infection. Acute respiratory disease (ARD), first recognized among military recruits during World War II, can be caused by adenovirus infections during conditions of crowding and stress. Adenoviruses are medium-sized (90-100 nm), nonenveloped icosohedral viruses containing double-stranded DNA. There are 49 immunologically distinct types (6 subgenera: A through F) that can cause human infections. Adenoviruses are unusually stable to chemical or physical agents and adverse pH conditions, allowing for prolonged survival outside of the body. Some adenoviruses, such as AD2 and Ad5 (species C) use clathrin mediated endocytosis and macropinocytosis for infectious entry. Other adenoviruses, such as Ad3 (species B) use dynamin dependent endocytosis and macropinocytosis for infectious entry.
In one embodiment the targeted virus is a respiratory syncytial virus (RSV). RSV is the most common cause of bronchiolitis and pneumonia among infants and children under 1 year of age. Illness begins most frequently with fever, runny nose, cough, and sometimes wheezing. During their first RSV infection, between 25% and 40% of infants and young children have signs or symptoms of bronchiolitis or pneumonia, and 0.5% to 2% require hospitalization. Most children recover from illness in 8 to 15 days. The majority of children hospitalized for RSV infection are under 6 months of age. RSV also causes repeated infections throughout life, usually associated with moderate-to-severe cold-like symptoms; however, severe lower respiratory tract disease can occur at any age, especially among the elderly or among those with compromised cardiac, pulmonary, or immune systems. RSV is a negative-sense, enveloped RNA virus. The virion is variable in shape and size (average diameter of between 120 and 300 nm), is unstable in the environment (surviving only a few hours on environmental surfaces), and is readily inactivated with soap and water and disinfectants.
In one embodiment the targeted virus is a human parainfluenza virus (HPIV). HPIVs are second to respiratory syncytial virus (RSV) as a common cause of lower respiratory tract disease in young children. Similar to RSV, HPIVs can cause repeated infections throughout life, usually manifested by an upper respiratory tract illness (e.g., a cold and/or sore throat). HPIVs can also cause serious lower respiratory tract disease with repeat infection (e.g., pneumonia, bronchitis, and bronchiolitis), especially among the elderly, and among patients with compromised immune systems. Each of the four HPIVs has different clinical and epidemiologic features. The most distinctive clinical feature of HPIV-1 and HPIV-2 is croup (i.e., laryngotracheobronchitis); HPIV-1 is the leading cause of croup in children, whereas HPIV-2 is less frequently detected. Both HPIV-1 and -2 can cause other upper and lower respiratory tract illnesses. HPIV-3 is more often associated with bronchiolitis and pneumonia. HPIV-4 is infrequently detected, possibly because it is less likely to cause severe disease. The incubation period for HPIVs is generally from 1 to 7 days. HPIVs are negative-sense, single-stranded RNA viruses that possess fusion and hemagglutinin-neuraminidase glycoprotein “spikes” on their surface. There are four serotypes types of HPIV (1 through 4) and two subtypes (4a and 4b). The virion varies in size (average diameter between 150 and 300 nm) and shape, is unstable in the environment (surviving a few hours on environmental surfaces), and is readily inactivated with soap and water.
In one embodiment the targeted virus is a coronavirus. Coronavirus is a genus of animal virus belonging to the family Coronaviridae. Coronaviruses are enveloped viruses with a positive-sense single-stranded RNA genome and a helical symmetry. The genomic size of coronaviruses ranges from approximately 16 to 31 kilobases, extraordinarily large for an RNA virus. The name “coronavirus” is derived from the Latin corona, meaning crown, as the virus envelope appears under electron microscopy to be crowned by a characteristic ring of small bulbous structures. This morphology is actually formed by the viral spike peplomers, which are proteins that populate the surface of the virus and determine host tropism. Coronaviruses are grouped in the order Nidovirales, named for the Latin nidus, meaning nest, as all viruses in this order produce a 3′ co-terminal nested set of subgenomic mRNAs during infection. Proteins that contribute to the overall structure of all coronaviruses are the spike, envelope, membrane and nucleocapsid. In the specific case of SARS a defined receptor-binding domain on S mediates the attachment of the virus to its cellular receptor, angiotensin-converting enzyme 2.
In one embodiment the targeted virus is a rhinovirus. Rhinovirus is a genus of the Picomaviridae family of viruses. Rhinoviruses are the most common viral infective agents in humans, and a causative agent of the common cold. There are over 105 serologic virus types that cause cold symptoms, and rhinoviruses are responsible for approximately 50% of all cases. Rhinoviruses have single-stranded positive sense RNA genomes of between 7.2 and 8.5 kb in length. At the 5′ end of the genome is a virus-encoded protein, and like mammalian mRNA, there is a 3′ poly-A tail. Structural proteins are encoded in the 5′ region of the genome and nonstructural at the end. This is the same for all picornaviruses. The viral particles themselves are not enveloped and are icosahedral in structure.
Any secondary structure of a viral protein (or of a host cell protein involved in viral infectivity) can form the basis of the methods. For example, a viral protein comprising a secondary structure which is a helix can be used to design peptidomimetic macrocycles based on the helix.
In one embodiment, the peptidomimetic macrocycle is designed based on the PB1 or PB2 sequence of an influenza virus. The PB1 sequence is highly conserved across all known strains of influenza A virus, which can result in less drug resistance should than that observed with the current standard of care. An alignment of the first 25 N-terminal amino acids of PB1 from the NCBI data bank's 2,485 influenza A virus strains (Ghanem, 2007) demonstrates the remarkable sequence conservation in the PA interaction domain of PB1. Therefore, antiviral therapies based on the PB1 sequence can block most, if not all, influenza A virus strains. Additionally, sequence modification of a peptidomimetic macrocycle based on these few variations in PB1 can enable an antiviral cocktail of PB1 inhibitors to eliminate resistance due to escape mutants.
Table 3a shows a list of peptidomimetic macrocycles derived from the PA-binding helix of PB1 that were prepared.
Table 3b shows a list of selected peptidomimetic macrocycles from Table 3a. SP-791 and SP-794 were prepared by increasing the length and alanine content (%) of the SP-786 sequence. These modifications led to a five-fold increase in antiviral activity compared to that of SP-786. SP-798 was prepared by incorporating an i, i+7 crosslink instead of the i, i+4 crosslink of SP-786. SP-192 exhibited improved anti-viral activity (EC₅₀=4.5 mM) compared to that of SP-786.
In some embodiments, the invention provides a peptidomimetic macrocycle that comprises an amino acid sequence that has at least 60%, 70%, 80%, 90%, 95%, 97%, or 10000 identity to any one of the amino acid sequences in Table 3a or 3b.

TABLE 3a

Prepared peptidomimetic macrocycles derived from the PA-binding helix of pB1.

SEQ

ID

SP#

NO:

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

780

1511

Ac-

Nle

D

V

N

$

T

L

$

L

K

V

Aib

A

Q

—NH₂

781

1512

Ac-

Nle

D

V

N

$

T

L

$

L

K

A

Aib

A

Q

—NH₂

782

1513

Ac-

Nle

D

V

N

$

T

L

$

L

K

V

Aib

A

—NH₂

783

1514

Ac-

Nle

D

V

N

$

T

L

$

L

K

V

P

Aib

Q

—NH₂

784

1515

Ac-

F

D

V

N

$

T

L

$

L

K

V

Aib

A

Q

—NH₂

785

1516

5-FAM-

Ba

Nle

D

V

N

$

T

L

$

L

A

V

Aib

A

Q

—NH₂

786

1517

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Aib

A

Q

—NH₂

787

1518

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Aib

A

Q

A

—NH₂

788

1519

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

A

Q

A

—NH₂

789

1520

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

A

—NH₂

790

1521

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

S

A

Q

A

—NH₂

791

1522

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

A

Q

A

—NH₂

792

1523

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

E

A

Q

A

—NH₂

793

1524

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

S

A

—NH₂

794

1525

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

A

—NH₂

795

1526

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

E

A

—NH₂

796

1527

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Aib

A

Q

A

a

—NH₂

797

1528

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Aib

A

Q

A

a

—OH

798

1529

Ac-

Nle

D

V

N

$r8

T

L

F

L

A

$

A

Q

—NH₂

799

1530

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

Aib

Q

A

—NH₂

800

1531

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

A

Q

Aib

A

—NH₂

801

1532

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

Aib

A

—NH₂

802

1533

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

A

Aib

A

—NH₂

803

1534

Ac-

Nle

D

V

N

$

T

L

$

L

A

V

Q

A

Aib

A

—NH₂

804

1535

Ac-

Nle

D

V

N

$r8

T

L

F

L

A

$

A

Q

A

—NH₂

805

1536

Ac-

Nle

D

V

N

$r8

T

L

F

L

A

$

A

—NH₂

806

1537

Ac-

Nle

D

V

N

$r8

T

L

F

L

A

$

Q

A

Q

A

—NH₂

807

1538

Ac-

Nle

D

V

N

$r8

T

L

F

L

A

$

Q

A

—NH₂

TABLE 3b

Selected peptidomimetic
macrocycles derived from the PA-binding helix of pB1.

							CPE,
SP#	Crosslink	Ch	L	VH	RT (min)*	Ala (%)	EC₅₀(μM)**

786	i, i + 4	−1	15	4.7	10.68	13	23
791	i, i + 4	−1	17	7.2	8.02	24	4.1
794	i, i + 4	−1	17	5.1	9.54	29	6.1
799	i, i + 4	−1	17	7		18	<6.1***
800	i, i + 4	−1	17	7	8.47	18	<6.1***
801	i, i + 4	−1	17	4.9	10.01	24	<6.1***
802	i, i + 4	−1	17	4.9	10.18	24	<6.1***
803	i, i + 4	−1	17	4.9	10.26	24	<6.1***
798	i, i + 7	−1	15	4.9	10.18	20	4.5
804	i, i + 7	−1	17	5.1	10.92	29	<4.5***
805	i, i + 7	−1	17	3	13.03	35	<4.5***
806	i, i + 7	−1	17	7.2	8.86	24	<4.5***
807	i, i + 7	−1	17	5.1	10.83	29	<4.5***

*See Example 11 table
**By Neutral Red assay (Influenza A Virus H1N1 California/07/2009)
***Predicted
Ch = net charge;
L = length in amino acids;
VH = von Heijne;
RT = retention time;
Ala = alanine content

In some embodiments of the invention, the peptide sequence is derived from the BCL-2 family of proteins. The BCL-2 family is defined by the presence of up to four conserved BCL-2 homology (BH) domains designated BH1, BH2, BH3, and BH4, all of which include α-helical segments (Chittenden et al. (1995), EMBO 14:5589; Wang et al. (1996), Genes Dev. 10:2859). Anti-apoptotic proteins, such as BCL-2 and BCL-X_L, display sequence conservation in all BH domains. Pro-apoptotic proteins are divided into “multidomain” family members (e.g., BAK, BAX), which possess homology in the BH1, BH2, and BH3 domains, and “BH3-domain only” family members (e.g., BID, BAD, BIM, BIK, NOXA, PUMA), that contain sequence homology exclusively in the BH3 amphipathic α-helical segment. BCL-2 family members have the capacity to form homo- and heterodimers, suggesting that competitive binding and the ratio between pro- and anti-apoptotic protein levels dictates susceptibility to death stimuli. Anti-apoptotic proteins function to protect cells from pro-apoptotic excess, i.e., excessive programmed cell death. Additional “security” measures include regulating transcription of pro-apoptotic proteins and maintaining them as inactive conformers, requiring either proteolytic activation, dephosphorylation, or ligand-induced conformational change to activate pro-death functions. In certain cell types, death signals received at the plasma membrane trigger apoptosis via a mitochondrial pathway. The mitochondria can serve as a gatekeeper of cell death by sequestering cytochrome c, a critical component of a cytosolic complex which activates caspase 9, leading to fatal downstream proteolytic events. Multidomain proteins such as BCL-2/BCL-X_Land BAK/BAX play dueling roles of guardian and executioner at the mitochondrial membrane, with their activities further regulated by upstream BH3-only members of the BCL-2 family. For example, BID is a member of the BH3-domain only family of pro-apoptotic proteins, and transmits death signals received at the plasma membrane to effector pro-apoptotic proteins at the mitochondrial membrane. BID has the capability of interacting with both pro- and anti-apoptotic proteins, and upon activation by caspase 8, triggers cytochrome c release and mitochondrial apoptosis. Deletion and mutagenesis studies determined that the amphipathic α-helical BH3 segment of pro-apoptotic family members can function as a death domain and thus can represent a critical structural motif for interacting with multidomain apoptotic proteins. Structural studies have shown that the BH3 helix can interact with anti-apoptotic proteins by inserting into a hydrophobic groove formed by the interface of BH1, 2 and 3 domains. Activated BID can be bound and sequestered by anti-apoptotic proteins (e.g., BCL-2 and BCL-X_L) and can trigger activation of the pro-apoptotic proteins BAX and BAK, leading to cytochrome c release and a mitochondrial apoptosis program. BAD is also a BH3-domain only pro-apoptotic family member whose expression triggers the activation of BAX/BAK. In contrast to BID, however, BAD displays preferential binding to anti-apoptotic family members, BCL-2 and BCL-X_L. Whereas the BAD BH3 domain exhibits high affinity binding to BCL-2, BAD BH3 peptide is unable to activate cytochrome c release from mitochondria in vitro, suggesting that BAD is not a direct activator of BAX/BAK. Mitochondria that over-express BCL-2 are resistant to BID-induced cytochrome c release, but co-treatment with BAD can restore BID sensitivity. Induction of mitochondrial apoptosis by BAD appears to result from either: (1) displacement of BAX/BAK activators, such as BID and BID-like proteins, from the BCL-2/BCL-X_Lbinding pocket, or (2) selective occupation of the BCL-2/BCL-X_Lbinding pocket by BAD to prevent sequestration of BID-like proteins by anti-apoptotic proteins. Thus, two classes of BH3-domain only proteins have emerged, BID-like proteins that directly activate mitochondrial apoptosis, and BAD-like proteins, that have the capacity to sensitize mitochondria to BID-like pro-apoptotics by occupying the binding pockets of multidomain anti-apoptotic proteins. Various α-helical domains of BCL-2 family member proteins amenable to the methodology disclosed herein have been disclosed (Walensky et al. (2004), Science 305:1466; and Walensky et al., U.S. Patent Publication No. 2005/0250680, the entire disclosures of which are incorporated herein by reference).
Myeloid cell leukemia 1 (MCL-1) is a protein that inhibits cell death through the binding and inhibition of pro-death factors such as BCL-2 interacting mediator (BIM). When MCL-1 is over-expressed, the rate of cell death in a cell or tissue is reduced. In some embodiments, the peptide sequences are derived from BIM. In some embodiments, a peptidomimetic macrocycle peptide derived from a human BIM peptide can be a peptide comprising 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 amino acids from a BIM peptide sequence.
In some embodiments, a peptidomimetic macrocycle peptide derived from a human BIM peptide sequence can be a peptide comprising 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 amino acids that are different from the selected sequences from which the peptide is derived. In some embodiments, a peptidomimetic macrocycle peptide derived from a human BIM peptide sequence can be a peptide comprising a mutation at amino acid position 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22. In some embodiments, mutations are mutations of non-essential amino acids. In some embodiments, mutations are mutations of essential amino acids. In some embodiments, mutations are mutations of hydrophobic amino acids. In some embodiments, mutations are mutations of naturally occurring amino acids. In some embodiments, mutations are mutations to a conservative amino acid. In some embodiments, a peptidomimetic macrocycle peptide derived from a human BIM peptide sequence can be a peptide comprising 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 amino acid analogues. In some embodiments, a peptidomimetic macrocycle peptide derived from a human BIM peptide sequence can be a peptide comprising 1 or 2 capping groups.
In some embodiments, the peptidomimetic macrocycle comprises a C-terminal truncation of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 amino acids from an amino acid sequence of BIM In some embodiments, the peptidomimetic macrocycle comprises a N-terminal truncation of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 amino acids from the sequence of BIM.
A non-limiting list of suitable BIM macrocycles for use in the present disclosure are given in Tables 4a and 4b. In Tables 4a and 4b, at the C-terminus, some peptides possess a carboxamide terminus (shown as —NH₂); some peptides possess a hydroxyl terminus (shown as —OH); some peptides possess a 5-carboxyfluorescein terminus (shown as -5-FAM); some peptides possess a isobutylamide terminus (shown as —NHiBu); some peptides possess a cyclohexylamide terminus (shown as —NHChx); some peptides possess a cyclohexylmethylamide terminus (shown as —NHMeChx); some peptides possess a phenethylamide terminus (shown as —NHPe); some peptides possess a n-butylamide terminus (shown as —NHBu); some peptides possess a sec-butylamide terminus (shown as —NHsBu); and some peptides possess an uncapped terminus (shown as no terminal modification).
In Tables 4a and 4b, at the N-terminus, some peptides possess an acetyl terminus (shown as Ac-); some peptides possess a fluorescein isothiocyanate terminus (shown as FITC-); some peptides possess a single-unit polyethylene glycol terminus (shown as dPEG1-); some peptides possess a five-unit polyethylene glycol terminus (shown as dPEG5-); some peptides possess an eleven-unit polyethylene glycol terminus (shown as dPEG11-); some peptides possess a propyl terminus (shown as Pr-); some peptides possess a biotin terminus (shown as Biotin-); some peptides possess a KLH terminus (shown as KLH-); some peptides possess an ovalbumin terminus (shown as OVA-); some peptides possess an uncapped terminus (shown as H-); some peptides possess a isobutyl terminus (shown as iBu-); some peptides possess a decanoyl terminus (shown as Decac-); some peptides possess a benzyl terminus (shown as Bz-); some peptides possess a cyclohexyl terminus (shown as Chx-); some peptides possess a benzyl terminus (shown as Bz-); some peptides possess a Vrl terminus (shown as Vrl-); some peptides possess a HBS terminus (shown as HBS-); some peptides possess a MeIm terminus (shown as MeImC-); some peptides possess a tert-butyl terminus (shown as t-Bu-U-); some peptides possess a nonanoyl terminus (shown as non-U-); some peptides possess a ethyl terminus (shown as Et-U-); some peptides possess a cyclohexyl terminus (shown as Chx-U-); some peptides possess a isopropyl terminus (shown as iPr-U-); some peptides possess a phenyl terminus (shown as Ph-U-); some peptides possess a uric terminus (shown as NH2CO-); some peptides possess a palmitoyl terminus (shown as Pam-); some peptides possess a heptenoic terminus (shown as Hep-); and some peptides possess a 5-carboxytetramethylrhodamine terminus (shown as 5-TAMRA-).
Table 4a shows a list of peptidomimetic macrocycles derived from the MCL-1/BCL-X_L/BCL-2-binding helix of BIM that were prepared. Table 4b shows a list of selected peptidomimetic macrocycles from Table 4a. SP-809 was prepared by incorporating an i, i+7 crosslink into the sequence of the linear peptide LP-2. SP-815 was prepared by removal of the two terminal arginine residues and an alanine substitution at position 13 of SP-809. SP-962 was prepared by a homoleucine substitution at position 9 and a F4F at position 17 of SP-815.
In some embodiments, the invention provides a peptidomimetic macrocycle that comprises an amino acid sequence that has at least 60%, 70%, 80%, 90%, 95%, 97%, or 100% identity to any one of the amino acid sequences in Table 4a or 4b.

TABLE 4a

Prepared peptidomimetic macrocycles derived from the MCL-1/BCL-X_L/BCL-2-binding helix of BIM

SP#	SEQ ID NO:			1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21		Calc. (M + 2)/2	Found mass

LP2

1539

Ac-

I

W

I

A

Q

E

L

R

I

G

D

E

F

N

A

Y

A

R

—NH₂

808

1540

Ac-

I

W

I

A

Q

E

L

R

$r8

I

G

D

E

F

N

$

Y

A

R

—NH₂

809

1541

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

E

F

N

$

Y

A

R

—NH₂

1344.74

1345.7

810

1542

Ac-

I

W

I

A

Q

E

L

R

$r8

I

G

D

E

F

N

$

Y

A

R

—NH₂

1373.75

1373.56

812

1543

Ac-

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1103.1

1103.12

813

1544

Ac-

I

A

Q

A

L

R

$r8

I

G

D

A

F

A

$

Y

A

—NH₂

988.55

988.45

814

1545

Ac-

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

964.04

963.94

815

1546

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1159.64

1159.87

816

1547

Ac-

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1103.1

1102.94

817

1548

Ac-

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1010.06

1009.9

818

1549

Ac-

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

981.55

981.86

819

1550

Ac-

I

A

Q

A

L

A

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

967.53

967.45

820

1551

Ac-

I

A

Q

A

L

R

$r8

I

A

D

A

F

N

$

Y

A

—NH₂

1017.07

1016.93

821

1552

Ac-

I

A

Q

A

L

R

$r8

I

G

D

A

N

$

Y

A

—NH₂

972.04

971.89

822

1553

Ac-

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

A

Y

A

—NH₂

964.04

963.94

823

1554

Ac-

I

$

I

A

Q

$

L

R

$r8

I

G

D

E

F

N

$

Y

A

—NH₂

1185.17

1185.61

824

1555

Ac-

I

W

I

A

Q

A

L

R

% r8

I

G

D

A

F

N

%

Y

A

—NH₂

1160.14

1161.28

825

1556

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

E

F

A

$

Y

A

—NH₂

1167.14

1168.2

826

1557

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

Q

A

N

$

Y

A

—NH₂

1150.13

1151.09

827

1558

FITC-

Ba

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1368.67

1369.79

828

1559

5-FAM-

Ba

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1353.18

1354.13

829

1560

5-FAM-

Ba

I

W

I

A

Q

A

L

R

$r8

I

G

D

E

F

N

$

Y

A

—NH₂

1382.18

1382.99

830

1561

Ac-

I

A

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1102.12

1103.17

831

1562

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

E

F

N

$

Y

A

—NH₂

1188.64

1189.57

832

1563

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

Q

F

N

$

Y

A

—NH₂

1188.15

1189.1

833

1564

Ac-

I

W

I

A

L

R

$r8

I

G

D

E

F

N

$

Y

A

—NH₂

1160.13

1161.17

834

1565

Ac-

I

W

I

A

L

R

$r8

I

G

D

Q

F

N

$

Y

A

—NH₂

1159.64

1160.34

835

1566

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1131.13

1132.12

836

1567

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

A

$

Y

A

—NH₂

1138.14

1139.15

837

1568

Ac-

I

W

I

A

Q

A

L

Cit

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1160.13

1160.98

838

1569

Ac-

I

W

I

A

Q

A

L

Cit

$r8

I

G

D

Q

F

N

$

Y

A

—NH₂

1188.64

1189.66

839

1570

Ac-

I

W

I

A

Q

A

L

H

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1150.12

1151.09

840

1571

Ac-

I

W

I

A

Q

A

L

H

$r8

I

G

D

Q

F

N

$

Y

A

—NH₂

1178.63

1179.67

841

1572

Ac-

I

W

I

A

Q

A

L

Q

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1145.62

1146.55

842

1573

Ac-

I

W

I

A

Q

A

L

Q

$r8

I

G

D

Q

F

N

$

Y

A

—NH₂

1174.13

1175.14

843

1574

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

N

$

Y

A

—NH₂

1121.62

1122.5

844

1575

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

I

N

$

Y

A

—NH₂

1142.65

1143.59

845

1576

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

Q

I

N

$

Y

A

—NH₂

1171.16

1171.9

846

1577

Ac-

I

W

I

A

Q

A

R

$r8

I

G

D

A

N

$

Y

A

—NH₂

1100.6

1101.5

847

1578

Ac-

I

W

I

A

Q

A

L

R

$r8

I

A

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.83

848

1579

Ac-

I

W

I

A

Q

A

L

R

$r8

I

A

D

Q

F

N

$

Y

A

—NH₂

1195.16

1196.23

849

1580

Ac-

I

W

I

A

Q

A

L

R

$r8

A

G

D

A

F

N

$

Y

A

—NH₂

1138.62

1139.61

850

1581

Ac-

I

W

I

A

Q

A

L

R

$r8

A

G

D

Q

F

N

$

Y

A

—NH₂

1167.13

1168.11

851

1582

Ac-

I

W

I

A

Q

A

L

R

$r8

F

G

D

A

F

N

$

Y

A

—NH₂

1176.63

1177.63

852

1583

Ac-

I

W

I

A

Q

A

L

R

$r8

F

G

D

Q

F

N

$

Y

A

—NH₂

1205.14

1205.94

853

1584

Ac-

I

W

F

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1176.63

1177.63

854

1585

Ac-

I

W

F

A

Q

A

L

R

$r8

I

G

D

Q

F

N

$

Y

A

—NH₂

1205.14

1206.13

855

1586

Ac-

I

W

I

A

Q

A

L

A

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1117.11

1118.15

856

1587

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

N

A

F

N

$

Y

A

—NH₂

1159.15

1159.63

857

1588

Ac-

I

W

I

A

Q

A

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1138.62

1139.2

858

1589

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

Q

F

A

$

Y

A

—NH₂

1166.65

1167.3

859

1590

Ac-

I

W

Cha

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1179.65

1180.15

860

1591

Ac-

I

W

hhL

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1173.65

1174.39

861

1592

Ac-

I

W

Adm

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1198.66

1199.28

862

1593

Ac-

I

W

hCha

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1186.66

1186.98

863

1594

Ac-

I

W

hF

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1183.64

1184.48

864

1595

Ac-

I

W

Igl

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1190.65

1190.41

865

1596

Ac-

I

W

F4CF3

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1210.62

1211.31

866

1597

Ac-

I

W

F4tBu

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1204.66

1205.39

867

1598

Ac-

I

W

2Nal

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1201.64

1202.2

868

1599

Ac-

I

W

Bip

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1214.65

1215.43

869

1600

Ac-

I

W

I

A

Q

A

Cha

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1179.65

1180.22

870

1601

Ac-

I

W

I

A

Q

A

hhL

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1173.65

1174.4

871

1602

Ac-

I

W

I

A

Q

A

Adm

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1198.66

1199.05

872

1603

Ac-

I

W

I

A

Q

A

hCha

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1186.66

1187.25

873

1604

Ac-

I

W

I

A

Q

A

hAdm

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1205.67

1206.4

874

1605

Ac-

I

W

I

A

Q

A

hF

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1183.64

1184.29

875

1606

Ac-

I

W

I

A

Q

A

Igl

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1190.65

1190.4

876

1607

Ac-

I

W

I

A

Q

A

F4CF3

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1210.62

1210.94

877

1608

Ac-

I

W

I

A

Q

A

F4tBu

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1204.66

1205.29

878

1609

Ac-

I

W

I

A

Q

A

2Nal

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1201.64

1202.15

879

1610

Ac-

I

W

I

A

Q

A

Bip

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1214.65

1214.91

880

1611

Ac-

I

W

I

A

Q

A

L

R

$r8

Cba

G

D

A

F

N

$

Y

A

—NH₂

1165.64

1166.07

881

1612

Ac-

I

W

I

A

Q

A

L

R

$r8

hL

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.37

882

1613

Ac-

I

W

I

A

Q

A

L

R

$r8

Cha

G

D

A

F

N

$

Y

A

—NH₂

1179.65

1180.22

883

1614

Ac-

I

W

I

A

Q

A

L

R

$r8

Tba

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.18

884

1615

Ac-

I

W

I

A

Q

A

L

R

$r8

hhL

G

D

A

F

N

$

Y

A

—NH₂

1173.65

1173.93

885

1616

Ac-

I

AmW

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.18

886

1617

Ac-

I

Aib

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1109.13

1109.46

887

1618

Ac-

AmL

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.27

888

1619

Ac-

I

W

AmL

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1137.37

889

1620

Ac-

I

W

I

Aib

Q

A

L

R

$r8

I

G

AmD

A

F

N

$

Y

A

—NH₂

1173.65

1173.93

890

1621

Ac-

I

W

I

A

Aib

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1138.14

1138.32

891

1622

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

AmD

A

F

N

$

Y

A

—NH₂

1166.65

1167.37

892

1623

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

F4F

A

—NH₂

1160.64

1161.45

896

1624

Ac-

I

W

Tba

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.37

897

1625

Ac-

I

W

hL

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.37

898

1626

Ac-

I

W

Chg

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1172.65

1173.47

899

1627

Ac-

I

W

Ac6c

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1165.64

1166.44

900

1628

Ac-

I

W

Ac5c

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1158.63

1159.32

901

1629

Ac-

E

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1139.11

1139.52

902

1630

Ac-

R

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1152.64

1153.49

903

1631

Ac-

K

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1138.63

1138.97

904

1632

Ac-

H

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1143.12

1143.87

905

1633

Ac-

S

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1118.1

1118.8

906

1634

Ac-

Q

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1138.62

1139.24

907

1635

Ac-

A

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1110.1

1110.75

908

1636

Ac-

Aib

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1117.11

1117.78

909

1637

Ac-

F

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1148.12

1148.96

910

1638

Ac-

I

D

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1095.6

1096.32

911

1639

Ac-

I

R

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1116.14

1116.95

912

1640

Ac-

I

H

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1106.62

1107.24

913

1641

Ac-

I

S

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1081.6

1181.98

914

1642

Ac-

I

N

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1095.11

1095.58

915

1643

Ac-

I

L

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1094.63

1095.3

916

1644

Ac-

I

F

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1111.62

1112.33

917

1645

Ac-

I

2Nal

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1136.63

1137.3

918

1646

Ac-

I

W

I

S

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1139.13

1139.89

919

1647

Ac-

I

W

I

L

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1152.15

1152.94

920

1648

Ac-

I

W

I

F

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1169.14

1169.86

921

1649

Ac-

I

W

I

A

L

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1152.15

1152.84

922

1650

Ac-

I

W

I

A

L

K

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1117.13

1117.97

923

1651

Ac-

I

W

I

A

L

R

$r8

I

Abu

D

A

F

N

$

Y

A

—NH₂

1145.14

1145.9

924

1652

Ac-

I

W

I

A

L

R

$r8

I

V

D

A

F

N

$

Y

A

—NH₂

1152.15

1152.94

925

1653

Ac-

I

W

I

A

L

R

$r8

I

G

E

A

F

N

$

Y

A

—NH₂

1138.14

1138.87

926

1654

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

G

N

$

Y

A

—NH₂

1086.1

1086.89

927

1655

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

W

N

$

Y

A

—NH₂

1179.14

1180.04

928

1656

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

hF

N

$

Y

A

—NH₂

1166.65

1167.46

929

1657

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F4CF3

N

$

Y

A

—NH₂

1193.63

1194.38

930

1658

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F4tBu

N

$

Y

A

—NH₂

1187.67

1188.36

931

1659

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

2Nal

N

$

Y

A

—NH₂

1184.65

1185.5

932

1660

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

Bip

N

$

Y

A

—NH₂

1197.65

1198.54

933

1661

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

D

$

Y

A

—NH₂

1131.62

1132.4

934

1662

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

E

$

Y

A

—NH₂

1138.63

1139.02

935

1663

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

Q

$

Y

A

—NH₂

1138.14

1138.84

936

1664

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

S

$

Y

A

—NH₂

1117.62

1118.5

937

1665

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

H

$

Y

A

—NH₂

1142.64

1143.25

938

1666

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

L

Y

A

—NH₂

1106.14

1107.05

939

1667

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1113.63

1114.27

940

1668

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

L

A

—NH₂

1134.65

1135.33

941

1669

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

Cha

A

—NH₂

1154.66

1155.31

942

1670

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

hF

A

—NH₂

1158.65

1159.5

943

1671

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

W

A

—NH₂

1171.15

1171.78

944

1672

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

2Nal

A

—NH₂

1176.65

1177

945

1673

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

D

—NH₂

1153.12

1153.77

946

1674

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

E

—NH₂

1160.13

1160.8

947

1675

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

Q

—NH₂

1159.64

1160.26

948

1676

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

S

—NH₂

1139.13

1139.47

949

1677

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

H

—NH₂

1164.14

1165.05

950

1678

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

R

—NH₂

1173.66

1174.4

951

1679

Ac-

I

W

I

A

L

R

$r8

I

G

D

A

F

N

$

Y

K

—NH₂

1159.66

1160.26

952

1680

Ac-

I

W

I

A

Q

A

AmL

R

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1166.65

1167.18

953

1681

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

AmD

A

F

N

$

Y

A

—NH₂

1166.65

1167.46

954

1682

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

F4F

Y

A

—NH₂

1160.64

1161.26

955

1683

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

Y

Aib

—NH₂

1166.65

1167.46

956

1684

Ac-

I

W

I

A

Q

A

Cit

$r8

I

G

D

A

F

N

$

Y

A

—NH₂

1139.11

1139.71

957

1685

Ac-

I

W

I

A

Q

A

L

Cit

$r8

I

G

N

A

F

N

$

Y

A

—NH₂

1159.64

1160.4

958

1686

Ac-

I

W

I

A

Q

A

L

Cit

$r8

I

G

D

A

N

$

Y

A

—NH₂

1122.12

1122.87

959

1687

Ac-

I

W

I

A

Q

A

L

Cit

$r8

I

G

D

A

V

N

$

Y

A

—NH₂

1136.13

1136.47

960

1688

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

A

F

N

$

A

Y

A

—NH₂

1113.63

1113.9

961

1689

Ac-

I

W

I

A

Q

A

L

R

$r8

hL

G

D

A

F

N

$

F4F

Y

A

—NH₂

1167.64

1168.57

962

1690

Ac-

I

W

I

A

Q

A

L

R

$r8

hL

G

D

A

F

N

$

Y

F4F

A

—NH₂

1167.64

1168.2

963

1691

Ac-

I

W

I

A

Q

A

L

R

$r8

hL

G

D

A

F

N

$

F4F

A

—NH₂

1168.64

1169.59

964

1692

Ac-

A

W

I

A

L

R

$r8

hL

G

D

A

F

N

$

Y

F4F

A

—NH₂

1118.11

1118.89

965

1693

Ac-

A

W

I

A

L

R

$r8

hL

G

D

A

F

N

$

A

F4F

A

—NH₂

1072.1

1072.92

966

1694

Ac-

I

W

I

A

Q

A

R

$r8

hL

G

D

A

F

N

$

F4F

A

—NH₂

1147.62

1148.59

893

1695

Ac-

I

$r8

I

A

Q

A

L

R

St

I

G

D

E

F

N

$s8

Y

A

—NH₂

1199.18

1199.74

894

1696

Ac-

I

W

I

A

$

A

L

R

St

I

G

D

E

F

N

$s8

Y

A

—NH₂

1207.17

1207.7

895

1697

Ac-

I

W

I

A

Q

A

L

R

$r8

I

G

D

E

F

N

St

Y

A

$r5

A

—NH₂

1306.72

1307.42

TABLE 4b

Selected peptidomimetic macrocycles derived from the MCL-1/BCL-X_L/BCL-2-binding helix
of BIM.

						Raji Cell
				RT	IC₅₀(nM)	Viability EC₅₀

SP#	Ch	L	VH	(min)*	Ala (%)	MCL-1	BCL-X_L	(μM)**

810	0	21	18.9	9.07	9.5	ND	9.2	>30
809	1	21	16.5	10.56	14.3	10.6	3.9	>30
815	0	19	9.1	15.07	21	8.4	22.4	6.6
962	0	19	8.3	17.69	21	27.0	13.0	0.7

*See Example 11 table
**5% serum, 48 hr
Ch = net charge;
L = length in amino acids;
VH = von Heijne;
RT = retention time;
Ala = alanine content

Preparation of Peptidomimetic Macrocycles

Peptidomimetic macrocycles can be prepared by any of a variety of methods known in the art. For example, any of the residues indicated by “$” or “$r8” in Table 1, Table 1a, Table 1b, or Table 1c can be substituted with a residue capable of forming a crosslinker with a second residue in the same molecule or a precursor of such a residue.
Various methods to effect formation of peptidomimetic macrocycles are known in the art. For example, the preparation of peptidomimetic macrocycles of Formula I is described in Schafmeister et al., J. Am. Chem. Soc. 122:5891-5892 (2000); Schafmeister & Verdine, J. Am. Chem. Soc. 122:5891 (2005); Walensky et al., Science 305:1466-1470 (2004); U.S. Pat. No. 7,192,713 and PCT application WO 2008/121767. The α,α-disubstituted amino acids and amino acid precursors disclosed in the cited references can be employed in synthesis of the peptidomimetic macrocycle precursor polypeptides. For example, the “S5-olefin amino acid” is (S)-α-(2′-pentenyl) alanine and the “R8 olefin amino acid” is (R)-α-(2′-octenyl) alanine. Following incorporation of such amino acids into precursor polypeptides, the terminal olefins are reacted with a metathesis catalyst, leading to the formation of the peptidomimetic macrocycle. In various embodiments, the following amino acids can be employed in the synthesis of the peptidomimetic macrocycle:
In other embodiments, the peptidomimetic macrocycles are of Formula IV or IVa. Methods for the preparation of such macrocycles are described, for example, in U.S. Pat. No. 7,202,332.
Additional methods of forming peptidomimetic macrocycles which are envisioned as suitable include those disclosed by Mustapa, M. Firouz Mohd et al., J. Org. Chem (2003), 68, pp. 8193-8198; Yang, Bin et al. Bioorg Med. Chem. Lett. (2004), 14, pp. 1403-1406; U.S. Pat. Nos. 5,364,851; 5,446,128; 5,824,483; 6,713,280; and 7,202,332. In such embodiments, amino acid precursors are used containing an additional substituent R— at the alpha position. Such amino acids are incorporated into the macrocycle precursor at the desired positions, which can be at the positions where the crosslinker is substituted or, alternatively, elsewhere in the sequence of the macrocycle precursor. Cyclization of the precursor is then effected according to the indicated method.

Assays

The properties of peptidomimetic macrocycles are assayed, for example, by using the methods described below. In some embodiments, a peptidomimetic macrocycle has improved biological properties relative to a corresponding polypeptide lacking the substituents described herein.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on one or more properties of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on a length of the amino acid sequence of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on a von Heijne value of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on a net charge carried by the polypeptide.
In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on an alanine content in the amino acid sequence of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on an amphipathicity of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on a solubility of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on a reverse-phase HPLC retention time of the polypeptide. In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on a length of the amino acid sequence of the polypeptide, a von Heijne value of the polypeptide, a net charge carried by the polypeptide, an alanine content in the amino acid sequence of the polypeptide, an amphipathicity of the polypeptide, a solubility of the polypeptide, a reverse-phase HPLC retention time of the polypeptide, or any combination thereof.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the length of the polypeptide. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 10-24 amino acids. For example, the length of the prepared peptidomimetic macrocycle is 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids, 18 amino acids, 19 amino acids, 20 amino acids, 21 amino acids, 22 amino acids, 23 amino acids, or 24 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 10-23, 10-22, 10-21, 10-20, 10-19, 10-18, 10-17, 10-16, 10-15, 10-14, 10-13, or 10-12 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 11-24, 12-24, 13-24, 14-24, 15-24, 16-24, 17-24, 18-24, 19-24, 20-24, 21-24, or 22-24 amino acids. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 11 amino acids to 23 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 11-22, 11-21, 11-20, 11-19, 11-18, 11-17, 11-16, 11-15, 11-14, or 11-13 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 12-23, 13-23, 14-23, 15-23, 16-23, 17-23, 18-23, 19-23, 20-23, or 21-23 amino acids. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 12 amino acids to 22 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 12-21, 12-20, 12-19, 12-18, 12-17, 12-16, 12-15, or 12-14 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 13-22, 14-22, 15-22, 16-22, 17-22, 18-22, 19-22, or 20-22 amino acids. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 13 amino acids to 21 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 13-20, 13-19, 13-18, 13-17, 13-16, or 13-15 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 14-21, 15-21, 16-21, 17-21, 18-21, or 19-21 amino acids. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 14 amino acids to 20 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from −19, 14-18, 14-17, or 14-16 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 15-20, 16-20, 17-20, or 18-20 amino acids. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 15 amino acids to 19 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 15-18 or 15-17 amino acids. For example, the length of the prepared peptidomimetic macrocycle ranges from 16-19 or 17-19 amino acids. In some embodiments, the length of the prepared peptidomimetic macrocycle ranges from 16 amino acids to 18 amino acids. For example, the length of the prepared peptidomimetic macrocycle is 17. In some embodiments, the length of the prepared peptidomimetic macrocycle is 14. In some embodiments, the length of the prepared peptidomimetic macrocycle is 15. In some embodiments, the length of the prepared peptidomimetic macrocycle is 16. In some embodiments, the length of the prepared peptidomimetic macrocycle is 17. In some embodiments, the length of the prepared peptidomimetic macrocycle is 18. In some embodiments, the length of the prepared peptidomimetic macrocycle is 19. In some embodiments, the length of the prepared peptidomimetic macrocycle is 20. In some embodiments, the length of the prepared peptidomimetic macrocycle is 21.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the von Heijne value of the polypeptide. In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 2 to 9. For example, the von Heijne value of the prepared peptidomimetic macrocycle is 2, 3, 4, 5, 6, 7, 8, or 9, along with all values in between. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 2-8, 2-7, 2-6, 2-5, 2-4, or 2-3. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 3-9, 4-9, 5-9, 6-9, 7-9, or 8-9. In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 3 to 8. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 3-7, 3-6, 3-5, or 3-4. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4-8, 5-8, 6-8, or 7-9.
In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4 to 7. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4-6 or 4-5. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 5-7 or 5-6. In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4-6. In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4-5. In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4.5-5.5, including 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, and 5.5 along with all values in between. In some embodiments, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4.5-9.5. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4.5-8.5, 4.5-7.5, 4.5-6.5, 5.5-9.5, 5.5-8.5, 5.5-7.5, 5.5-6.5, 6.5-9.5, 6.5-8.5, 6.5-7.5, 7.5-9.5, or 7.5-8.5.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the net charge carried by a peptide. For example, peptidomimetic macrocycles carrying a high number of negative charges can have poor cell permeability. A peptidomimetic macrocycle carrying a high number of positive charges can have good cell permeability, but can cause undesired cell damage (e.g., cell lysis). A prepared peptidomimetic macrocycle can carry a net charge such that the polypeptide is cell permeable, but not damaging to cells (e.g., does not cause cell lysis). In some embodiments, the net charge of the prepared peptidomimetic macrocycle ranges from −4 to +2, including −4, −3, −2, −1, 0, +1, and +2. In some embodiments, the net charge of the prepared peptidomimetic macrocycle ranges from −3 to +1, including −3, −2, −1, 0 and +1.
In some embodiments, the net charge of the prepared peptidomimetic macrocycle ranges from −2 to 0, including −2, −1, and 0. In some embodiments, the net charge of the prepared peptidomimetic macrocycle is zero or negative. In some embodiments, the net charge of the prepared peptidomimetic macrocycle is not positive. In some embodiments, the net charge of the prepared peptidomimetic macrocycle is zero or is not positive. In some embodiments, the net charge of the prepared peptidomimetic macrocycle is −2. In some embodiments, the net charge of the prepared peptidomimetic macrocycle is −1. In some embodiments, the net charge of the prepared peptidomimetic macrocycle is 0.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the alanine content of the polypeptide. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 15% to 50%. For example, the alanine content of the prepared peptidomimetic macrocycle can be 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, and 50%, along with all values in between. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 15% to 45%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 15% to 40%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 15% to 35%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 20% to 50%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 20% to 45%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 20% to 40%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 20% to 35%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 20% to 30%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 20% to 25%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 50%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 45%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 40%. For example, the alanine content of the prepared peptidomimetic macrocycle can be 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, and 40% along with all values in between. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 35%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 30%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 30% to 50%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 30% to 45%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 30% to 40%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 30% to 35%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 35% to 50%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 35% to 45%. In some embodiments, the alanine content of the prepared peptidomimetic macrocycle ranges from 35% to 40%.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the length and the von Heijne value of the polypeptide. For example, the length of the prepared peptidomimetic macrocycle ranges from 10 amino acids to 24 amino acids, from 11 amino acids to 23 amino acids, from 12 amino acids to 22 amino acids, from 13 amino acids to 21 amino acids, from 14 amino acids to 20 amino acids, from 15 amino acids to 19 amino acids, or from 16 amino acids to 18 amino acids, and the von Heijne value of the prepared peptidomimetic macrocycle ranges from 2 to 9, from 3 to 8, from 4 to 7, from 4 to 6, or from 4 to 5. For example, the length of the prepared peptidomimetic macrocycle is 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids, 18 amino acids, 19 amino acids, 20 amino acids, or 21 amino acids, and the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4.5 to 5.5. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, and a von Heijne value ranging from 4 and 7.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the length and the alanine content of the polypeptide. For example, the length of the prepared peptidomimetic macrocycle ranges from 10 amino acids to 24 amino acids, from 11 amino acids to 23 amino acids, from 12 amino acids to 22 amino acids, from 13 amino acids to 21 amino acids, from 14 amino acids to 20 amino acids, from 15 amino acids to 19 amino acids, or from 16 amino acids to 18 amino acids, and the alanine content of the prepared peptidomimetic macrocycle ranges from 15% to 50%, including 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, and 50% along with all values in between. For example, the length of the prepared peptidomimetic macrocycle is 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids, 18 amino acids, 19 amino acids, 20 amino acids, or 21 amino acids, and the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 40%, including 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, and 40% along with all values in between. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, and an alanine content ranging from 25% to 40%.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the length and the net charge of the polypeptide. For example, the length of the prepared peptidomimetic macrocycle ranges from 10 amino acids to 24 amino acids, from 11 amino acids to 23 amino acids, from 12 amino acids to 22 amino acids, from 13 amino acids to 21 amino acids, from 14 amino acids to 20 amino acids, from 15 amino acids to 19 amino acids, or from 16 amino acids to 18 amino acids, and the net charge of the prepared peptidomimetic macrocycle ranges from −3 to 1, including −3, −2, −1, 0 and 1. For example, the length of the prepared peptidomimetic macrocycle is 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids, 18 amino acids, 19 amino acids, 20 amino acids, or 21 amino acids, and the net charge of the prepared peptidomimetic macrocycle ranges from −3 to 1, including −3, −2, −1, 0 and 1. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, and a net charge ranging from −2 to 0.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the von Heijne value and the net charge of the polypeptide. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 2 to 9, from 3 to 8, from 4 to 7, from 4 to 6, or from 4 to 5, and the net charge of the prepared peptidomimetic macrocycle ranges from −3 to 1, including −3, −2, −1, 0 and 1. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4.5 to 5.5, including 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, and 5.5 along with all values in between, and the net charge of the prepared peptidomimetic macrocycle ranges from −3 to 1, including −3, −2, −1, 0 and 1. For example, the prepared peptidomimetic macrocycle has a von Heijne value ranging from 4 and 7, and a net charge ranging from −2 to 0.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the von Heijne value and the alanine content of the polypeptide. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 2 to 9, from 3 to 8, from 4 to 7, from 4 to 6, or from 4 to 5, and the alanine content of the prepared peptidomimetic macrocycle ranges from 15% to 50%, including 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, and 50% along with all values in between. For example, the von Heijne value of the prepared peptidomimetic macrocycle ranges from 4.5 to 5.5, including 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, and 5.5 along with all values in between, and the alanine content of the prepared peptidomimetic macrocycle ranges from 25% to 40%, including 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, and 40% along with all values in between. For example, the prepared peptidomimetic macrocycle has a von Heijne value ranging from 4 and 7, and an alanine content ranging from 25% to 40%.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on length, von Heijne value and alanine content of the polypeptide. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, a von Heijne value ranging from 4 and 7, and an alanine content ranging from 25% to 40%.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the von Heijne value and the net charge of the polypeptide. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, a von Heijne value ranging from 4 and 7, and a net charge ranging from −2 to 0.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the von Heijne value, the net charge, and the alanine content of the polypeptide. For example, the prepared peptidomimetic macrocycle has a von Heijne value ranging from 4 and 7, a net charge ranging from −2 to 0, and an alanine content ranging from 25% to 40%.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the length, the net charge, and the alanine content of the polypeptide. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, a net charge ranging from −2 to 0, and an alanine content ranging from 25% to 40%.
A peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the length of its amino acid sequence, its von Heijne value, its net charge, and the alanine content of its amino acid sequence. For example, the prepared peptidomimetic macrocycle has a length ranging from 14 amino acids to 20 amino acids, a von Heijne value ranging from 4 and 7, a net charge ranging from −2 to 0, and an alanine content ranging from 25% to 40%.
In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on the reverse-phase HPLC retention time of the polypeptide.
In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on amphipathicity of the polypeptide.
In some embodiments, a peptidomimetic macrocycle with enhanced cell penetrability can be prepared based on solubility of the polypeptide, for example if the prepared peptidomimetic macrocycle is determined to be soluble based on visual examination of the turbidity of a solution of the polypeptide.
Assay to Determine α-Helicity
In solution, the secondary structure of polypeptides with α-helical domains will reach a dynamic equilibrium between random coil structures and α-helical structures, often expressed as a “percent helicity”. Thus, for example, alpha-helical domains are predominantly random coils in solution, with α-helical content usually under 25%. Peptidomimetic macrocycles with optimized linkers, on the other hand, possess, for example, an alpha-helicity that is at least two-fold greater than that of a corresponding uncrosslinked polypeptide. In some embodiments, macrocycles will possess an alpha-helicity of greater than 50%. To assay the helicity of peptidomimetic macrocycles, the compounds are dissolved in an aqueous solution (e.g. 50 mM potassium phosphate solution at pH 7, or distilled H₂O, to concentrations of 25-50 μM). Circular dichroism (CD) spectra are obtained on a spectropolarimeter (e.g., Jasco J-710) using standard measurement parameters (e.g. temperature, 20° C.; wavelength, 190-260 nm; step resolution, 0.5 nm; speed, 20 nm/sec; accumulations, 10; response, 1 sec; bandwidth, 1 nm; path length, 0.1 cm). The α-helical content of each peptide is calculated by dividing the mean residue ellipticity (e.g. [Φ]222obs) by the reported value for a model helical decapeptide (Yang et al. (1986), Methods Enzymol. 130:208)).
Assay to Determine Melting Temperature (Tm)
A peptidomimetic macrocycle comprising a secondary structure such as an α-helix exhibits, for example, a higher melting temperature than a corresponding uncrosslinked polypeptide. Typically peptidomimetic macrocycles exhibit Tm of >60° C. representing a highly stable structure in aqueous solutions. To assay the effect of macrocycle formation on melting temperature, peptidomimetic macrocycles or unmodified peptides are dissolved in distilled H₂O (e.g. at a final concentration of 50 μM) and the Tm is determined by measuring the change in ellipticity over a temperature range (e.g. 4 to 95° C.) on a spectropolarimeter (e.g., Jasco J-710) using standard parameters (e.g. wavelength 222 nm; step resolution, 0.5 nm; speed, 20 nm/sec; accumulations, 10; response, 1 sec; bandwidth, 1 nm; temperature increase rate: 1° C./min; path length, 0.1 cm).
Protease Resistance Assay
The amide bond of the peptide backbone is susceptible to hydrolysis by proteases, thereby rendering peptidic compounds vulnerable to rapid degradation in vivo. Peptide helix formation, however, typically buries the amide backbone and therefore can shield it from proteolytic cleavage. The peptidomimetic macrocycles can be subjected to in vitro trypsin proteolysis to assess for any change in degradation rate compared to a corresponding uncrosslinked polypeptide. For example, the peptidomimetic macrocycle and a corresponding uncrosslinked polypeptide are incubated with trypsin agarose and the reactions quenched at various time points by centrifugation and subsequent HPLC injection to quantitate the residual substrate by ultraviolet absorption at 280 nm. Briefly, the peptidomimetic macrocycle and peptidomimetic precursor (5 mcg) are incubated with trypsin agarose (Pierce) (S/E˜125) for 0, 10, 20, 90, and 180 minutes. Reactions are quenched by tabletop centrifugation at high speed; remaining substrate in the isolated supernatant is quantified by HPLC-based peak detection at 280 nm. The proteolytic reaction displays first order kinetics and the rate constant, k, is determined from a plot of ln[S] versus time (k=−1×slope).
Ex Vivo Stability Assay
Peptidomimetic macrocycles with optimized linkers possess, for example, an ex vivo half-life that is at least two-fold greater than that of a corresponding uncrosslinked polypeptide, and possess an ex vivo half-life of 12 hours or more. For ex vivo serum stability studies, a variety of assays can be used. For example, a peptidomimetic macrocycle and a corresponding uncrosslinked polypeptide (2 mcg) are incubated with fresh mouse, rat and/or human serum (2 mL) at 37° C. for 0, 1, 2, 4, 8, and 24 hours. To determine the level of intact compound, the following procedure can be used: The samples are extracted by transferring 100 μl of sera to 2 ml centrifuge tubes followed by the addition of 10 μL of 50% formic acid and 500 μL acetonitrile and centrifugation at 14,000 RPM for 10 min at 4±2° C. The supernatants are then transferred to fresh 2 ml tubes and evaporated on Turbovap under N₂<10 psi, 37° C. The samples are reconstituted in 100 μL of 50:50 acetonitrile:water and submitted to LC-MS/MS analysis.
In Vitro Binding Assays
To assess the binding and affinity of peptidomimetic macrocycles and peptidomimetic precursors to acceptor proteins, a fluorescence polarization assay (FPA) is used, for example. The FPA technique measures the molecular orientation and mobility using polarized light and fluorescent tracer. When excited with polarized light, fluorescent tracers (e.g., FITC) attached to molecules with high apparent molecular weights (e.g. FITC-labeled peptides bound to a large protein) emit higher levels of polarized fluorescence due to their slower rates of rotation as compared to fluorescent tracers attached to smaller molecules (e.g. FITC-labeled peptides that are free in solution).
For example, fluoresceinated peptidomimetic macrocycles (25 nM) are incubated with the acceptor protein (25-1000 nM) in binding buffer (140 mM NaCl, 50 mM Tris-HCL, pH 7.4) for 30 minutes at room temperature. Binding activity is measured, for example, by fluorescence polarization on a luminescence spectrophotometer (e.g. Perkin-Elmer LS50B). Kd values can be determined by nonlinear regression analysis using, for example, Graphpad Prism software (GraphPad Software, Inc., San Diego, Calif.). A peptidomimetic macrocycle shows, In some embodiments, similar or lower Kd than a corresponding uncrosslinked polypeptide.
In Vitro Displacement Assays to Characterize Antagonists of Peptide-Protein Interactions
To assess the binding and affinity of compounds that antagonize the interaction between a peptide and an acceptor protein, a fluorescence polarization assay (FPA) utilizing a fluoresceinated peptidomimetic macrocycle derived from a peptidomimetic precursor sequence is used, for example. The FPA technique measures the molecular orientation and mobility using polarized light and fluorescent tracer. When excited with polarized light, fluorescent tracers (e.g., FITC) attached to molecules with high apparent molecular weights (e.g. FITC-labeled peptides bound to a large protein) emit higher levels of polarized fluorescence due to their slower rates of rotation as compared to fluorescent tracers attached to smaller molecules (e.g. FITC-labeled peptides that are free in solution). A compound that antagonizes the interaction between the fluoresceinated peptidomimetic macrocycle and an acceptor protein will be detected in a competitive binding FPA experiment.
For example, putative antagonist compounds (1 nM to 1 mM) and a fluoresceinated peptidomimetic macrocycle (25 nM) are incubated with the acceptor protein (50 nM) in binding buffer (140 mM NaCl, 50 mM Tris-HCL, pH 7.4) for 30 minutes at room temperature. Antagonist binding activity is measured, for example, by fluorescence polarization on a luminescence spectrophotometer (e.g. Perkin-Elmer LS50B). Kd values can be determined by nonlinear regression analysis using, for example, Graphpad Prism software (GraphPad Software, Inc., San Diego, Calif.).
Any class of molecule, such as small organic molecules, peptides, oligonucleotides or proteins can be examined as putative antagonists in this assay.

Assay for Protein-Ligand Binding by Affinity Selection-Mass Spectrometry

To assess the binding and affinity of test compounds for proteins, an affinity-selection mass spectrometry assay is used, for example. Protein-ligand binding experiments are conducted according to the following representative procedure outlined for a system-wide control experiment using 1 μM peptidomimetic macrocycle plus 5 μM hMDM2. A 1 μL DMSO aliquot of a 40 μM stock solution of peptidomimetic macrocycle is dissolved in 19 μL of PBS (Phosphate-buffered saline: 50 mM, pH 7.5 Phosphate buffer containing 150 mM NaCl). The resulting solution is mixed by repeated pipetting and clarified by centrifugation at 10 000 g for 10 min. To a 4 μL aliquot of the resulting supernatant is added 4 μL of 10 μM hMDM2 in PBS. Each 8.0 μL experimental sample thus contains 40 pmol (1.5 μg) of protein at 5.0 μM concentration in PBS plus 1 μM peptidomimetic macrocycle and 2.5% DMSO. Duplicate samples thus prepared for each concentration point are incubated for 60 min at room temperature, and then chilled to 4° C. prior to size-exclusion chromatography-LC-MS analysis of 5.0 μL injections. Samples containing a target protein, protein-ligand complexes, and unbound compounds are injected onto an SEC column, where the complexes are separated from non-binding component by a rapid SEC step. The SEC column eluate is monitored using UV detectors to confirm that the early-eluting protein fraction, which elutes in the void volume of the SEC column, is well resolved from unbound components that are retained on the column. After the peak containing the protein and protein-ligand complexes elutes from the primary UV detector, it enters a sample loop where it is excised from the flow stream of the SEC stage and transferred directly to the LC-MS via a valving mechanism. The (M+3H)³⁺ ion of the peptidomimetic macrocycle is observed by ESI-MS at the expected m/z, confirming the detection of the protein-ligand complex.
Assay for Protein-Ligand Kd Titration Experiments
To assess the binding and affinity of test compounds for proteins, a protein-ligand Kd titration experiment is performed, for example. Protein-ligand K_dtitrations experiments are conducted as follows: 2 μL DMSO aliquots of a serially diluted stock solution of titrant peptidomimetic macrocycle (5, 2.5, . . . , 0.098 mM) are prepared then dissolved in 38 μL of PBS. The resulting solutions are mixed by repeated pipetting and clarified by centrifugation at 10.000 g for 10 min. To 4.0 μL aliquots of the resulting supernatants is added 4.0 μL of 10 μM hMDM2 in PBS. Each 8.0 μL experimental sample thus contains 40 pmol (1.5 μg) of protein at 5.0 μM concentration in PBS, varying concentrations (125, 62.5, . . . , 0.24 μM) of the titrant peptide, and 2.5% DMSO. Duplicate samples thus prepared for each concentration point are incubated at room temperature for 30 min, then chilled to 4° C. prior to SEC-LC-MS analysis of 2.0 μL injections. The (M+H)¹⁺, (M+2H)²⁺, (M+3H)³⁺ and/or (M+Na)¹⁺ ion is observed by ESI-MS; extracted ion chromatograms are quantified, then fit to equations to derive the binding affinity K_das described in “A General Technique to Rank Protein-Ligand Binding Affinities and Determine Allosteric vs. Direct Binding Site Competition in Compound Mixtures.” Annis, D. A.; Nazef, N.; Chuang, C. C.; Scott, M. P.; Nash, H. M. J. Am. Chem. Soc. 2004, 126, 15495-15503; also in “ALIS: An Affinity Selection-Mass Spectrometry System for the Discovery and Characterization of Protein-Ligand Interactions” D. A. Annis, C.-C. Chuang, and N. Nazef. In Mass Spectrometry in Medicinal Chemistry. Edited by Wanner K, Hofner G: Wiley-VCH; 2007:121-184. Mannhold R, Kubinyi H, Folkers G (Series Editors): Methods and Principles in Medicinal Chemistry.
Assay for Competitive Binding Experiments by Affinity Selection-Mass Spectrometry
To determine the ability of test compounds to bind competitively to proteins, an affinity selection mass spectrometry assay is performed, for example. A mixture of ligands at 40 μM per component is prepared by combining 2 μL aliquots of 400 μM stocks of each of the three compounds with 14 μL of DMSO. Then, 1 μL aliquots of this 40 μM per component mixture are combined with 1 μL DMSO aliquots of a serially diluted stock solution of titrant peptidomimetic macrocycle (10, 5, 2.5, . . . , 0.078 mM). These 2 μL samples are dissolved in 38 μL of PBS. The resulting solutions were mixed by repeated pipetting and clarified by centrifugation at 10 000 g for 10 min. To 4.0 μL aliquots of the resulting supernatants is added 4.0 μL of 10 μM hMDM2 protein in PBS. Each 8.0 μL experimental sample thus contains 40 pmol (1.5 μg) of protein at 5.0 μM concentration in PBS plus 0.5 μM ligand, 2.5% DMSO, and varying concentrations (125, 62.5, . . . , 0.98 μM) of the titrant peptidomimetic macrocycle. Duplicate samples thus prepared for each concentration point are incubated at room temperature for 60 min, then chilled to 4° C. prior to SEC-LC-MS analysis of 2.0 μL injections. Additional details on these and other methods are provided in “A General Technique to Rank Protein-Ligand Binding Affinities and Determine Allosteric vs. Direct Binding Site Competition in Compound Mixtures.” Annis, D. A.; Nazef, N.; Chuang, C. C.; Scott, M. P.; Nash, H. M. J. Am. Chem. Soc. 2004, 126, 15495-15503; also in “ALIS: An Affinity Selection-Mass Spectrometry System for the Discovery and Characterization of Protein-Ligand Interactions” D. A. Annis, C.-C. Chuang, and N. Nazef. In Mass Spectrometry in Medicinal Chemistry. Edited by Wanner K, Höfner G: Wiley-VCH; 2007:121-184. Mannhold R, Kubinyi H, Folkers G (Series Editors): Methods and Principles in Medicinal Chemistry.
Binding Assays in Intact Cells
It is possible to measure binding of peptides or peptidomimetic macrocycles to their natural acceptors in intact cells by immunoprecipitation experiments. For example, intact cells are incubated with fluoresceinated (FITC-labeled) compounds for 4 hrs in the absence of serum, followed by serum replacement and further incubation that ranges from 4-18 hrs. Cells are then pelleted and incubated in lysis buffer (50 mM Tris [pH 7.6], 150 mM NaCl, 1% CHAPS and protease inhibitor cocktail) for 10 minutes at 4° C. Extracts are centrifuged at 14,000 rpm for 15 minutes and supernatants collected and incubated with 10 μl goat anti-FITC antibody for 2 hrs, rotating at 4° C. followed by further 2 hrs incubation at 4° C. with protein A/G Sepharose (50 μl of 50% bead slurry). After quick centrifugation, the pellets are washed in lysis buffer containing increasing salt concentration (e.g., 150, 300, 500 mM). The beads are then re-equilibrated at 150 mM NaCl before addition of SDS-containing sample buffer and boiling. After centrifugation, the supernatants are optionally electrophoresed using 4%-12% gradient Bis-Tris gels followed by transfer into Immobilon-P membranes. After blocking, blots are optionally incubated with an antibody that detects FITC and also with one or more antibodies that detect proteins that bind to the peptidomimetic macrocycle.
Cellular Penetrability Assays
A peptidomimetic macrocycle is, for example, more cell penetrable compared to a corresponding uncrosslinked macrocycle. Peptidomimetic macrocycles with optimized linkers possess, for example, cell penetrability that is at least two-fold greater than a corresponding uncrosslinked macrocycle, and often 20% or more of the applied peptidomimetic macrocycle will be observed to have penetrated the cell after 4 hours. To measure the cell penetrability of peptidomimetic macrocycles and corresponding uncrosslinked macrocycle, intact cells are incubated with fluorescently-labeled (e.g. fluoresceinated) peptidomimetic macrocycles or corresponding uncrosslinked macrocycle (10 μM) for 4 hrs in serum free media at 37° C., washed twice with media and incubated with trypsin (0.25%) for 10 min at 37° C. The cells are washed again and resuspended in PBS. Cellular fluorescence is analyzed, for example, by using either a FACSCalibur flow cytometer or Cellomics' KineticScan® HCS Reader.
Cellular Efficacy Assays
The efficacy of certain peptidomimetic macrocycles is determined, for example, in cell-based killing assays using a variety of tumorigenic and non-tumorigenic cell lines and primary cells derived from human or mouse cell populations. Cell viability is monitored, for example, over 24-96 hrs of incubation with peptidomimetic macrocycles (0.5 to 50 μM) to identify those that kill at EC₅₀<10 μM. Several standard assays that measure cell viability are commercially available and are optionally used to assess the efficacy of the peptidomimetic macrocycles. In addition, assays that measure Annexin V and caspase activation are optionally used to assess whether the peptidomimetic macrocycles kill cells by activating the apoptotic machinery. For example, the Cell Titer-glo assay is used which determines cell viability as a function of intracellular ATP concentration.
In Vivo Stability Assay
To investigate the in vivo stability of the peptidomimetic macrocycles, the compounds are, for example, administered to mice and/or rats by IV, IP, PO or inhalation routes at concentrations ranging from 0.1 to 50 mg/kg and blood specimens withdrawn at 0′, 5′, 15′, 30′, 1 hr, 4 hrs, 8 hrs and 24 hours post-injection. Levels of intact compound in 25 μL of fresh serum are then measured by LC-MS/MS as above.
In Vivo Efficacy in Animal Models
To determine the anti-oncogenic activity of peptidomimetic macrocycles in vivo, the compounds are, for example, given alone (IP, IV, PO, by inhalation or nasal routes) or in combination with sub-optimal doses of relevant chemotherapy (e.g., cyclophosphamide, doxorubicin, etoposide). In one example, 5×10⁶RS4;11 cells (established from the bone marrow of a patient with acute lymphoblastic leukemia) that stably express luciferase are injected by tail vein in NOD-SCID mice 3 hrs after they have been subjected to total body irradiation. If left untreated, this form of leukemia is fatal in 3 weeks in this model. The leukemia is readily monitored, for example, by injecting the mice with D-luciferin (60 mg/kg) and imaging the anesthetized animals (e.g., Xenogen In Vivo Imaging System, Caliper Life Sciences, Hopkinton, Mass.). Total body bioluminescence is quantified by integration of photonic flux (photons/sec) by Living Image Software (Caliper Life Sciences, Hopkinton, Mass.). Peptidomimetic macrocycles alone or in combination with sub-optimal doses of relevant chemotherapeutics agents are, for example, administered to leukemic mice (10 days after injection/day 1 of experiment, in bioluminescence range of 14-16) by tail vein or IP routes at doses ranging from 0.1 mg/kg to 50 mg/kg for 7 to 21 days. Optionally, the mice are imaged throughout the experiment every other day and survival monitored daily for the duration of the experiment. Expired mice are optionally subjected to necropsy at the end of the experiment. Another animal model is implantation into NOD-SCID mice of DoHH2, a cell line derived from human follicular lymphoma, that stably expresses luciferase. These in vivo tests optionally generate preliminary pharmacokinetic, pharmacodynamic and toxicology data.
Clinical Trials
To determine the suitability of the peptidomimetic macrocycles for treatment of humans, clinical trials are performed. For example, patients diagnosed with solid tumor and in need of treatment can be selected and separated in treatment and one or more control groups, wherein the treatment group is administered a peptidomimetic macrocycle, while the control groups receive a placebo or a known anti-cancer drug. The treatment safety and efficacy of the peptidomimetic macrocycles can thus be evaluated by performing comparisons of the patient groups with respect to factors such as survival and quality-of-life. In this example, the patient group treated with a peptidomimetic macrocycle can show improved long-term survival compared to a patient control group treated with a placebo.
Chemical Stability
To assay the chemical stability of the aqueous pharmaceutical formulations disclosed herein, 1 mL of the aqueous pharmaceutical formulation is filled in 2-mL vials with 13-mm Ø stoppers. The smaller vial size can help provide a greater surface-to-volume ratio which would amplify any container/closure effects on product stability. To assure that all surfaces of the vials were challenged, the vials can be stored in an inverted position. The vials are stored at the desired assay temperature, for example −20° C., 5° C., 20° C., and 40° C. for the desired assay time. For example for 1, 2, 3 or 6 months. The samples are analyzed by reverse phase HPLC. Tables 8-12 and 14-17 depict the results of this study. The samples can also be analyzed for particulate matter.
In Vitro Testing for Inhibition of Influenza Replication
This influenza antiviral evaluation assay examines the effects of compounds at designated dose-response concentrations. See also Noah, J. W., W. Severson, D. L. Noah, L. Rasmussen, E. L. White, and C. B. Jonsson, Antiviral Res, 2007. 73(1): p. 50-9. Madin Darby canine kidney (MDCK) cells are used in the assay to test the efficacy of the compounds in preventing the cytopathic effect (CPE) induced by influenza infection. Either Ribavirin or Tamiflu is included in each run as a positive control compound. Subconfluent cultures of MDCK cells are plated into 96-well plates for the analysis of cell viability (cytotoxicity) and antiviral activity (CPE). Drugs are added to the cells 24 hr later. At a designated time, the CPE wells also receive 100 tissue culture infectious doses (100 TCID50s) of titered influenza virus. 72 hr later the cell viability is determined. The effective compound concentrations which reduce viral-induced CPE by 25% (IC25), 50% (IC50), and 90% (IC90) are calculated by regression analysis with semi-log curve fitting. Cell viability is assessed using CellTiter-Glo (Promega). The toxic concentration of drug that reduces cell numbers by 50% and 90% (TC50 and TC90, respectively) are calculated as well. Selectivity (therapeutic) indices (SI=TC/IC) are also calculated.
In Vivo Testing for Inhibition of Influenza Replication
In vivo testing of compounds can be performed, including testing on mammals such as rats or ferrets. Because ferrets (Mustela putorius furo) are naturally susceptible to infection with human influenza A and B viruses and their disease resembles that of human influenza, these animals have been widely used as a model for influenza virus pathogenesis and immunity studies. See Sidwell, R. W. and D. F. Smee, Antiviral Res, 2000. 48(1): p. 1-16; and Colacino, J. M., D. C. DeLong, J. R. Nelson, W. A. Spitzer, J. Tang, F. Victor, and C. Y. Wu, Antimicrob Agents Chemother, 1990. 34(11): p. 2156-63. Ferrets are also the model of choice for the study of avian influenza virus H5N1 pathogenesis in mammals. See also Zitzow, L. A., T. Rowe, T. Morken, W.-J. Shieh, S. Zaki, and J. M. Katz, Pathogenesis of Avian Influenza A (H5N1) Viruses in Ferrets. 2002. p. 4420-4429. The activities of the PB1 Stapled Peptides can be compared to Ribavirin or Oseltamivir as a positive control.
Briefly, young adult male or female ferrets (five ferrets for each treatment group) that are serologically negative by hemagglutination inhibition assay for currently circulating human influenza A or B viruses are quarantined at least 4 days prior to infection in a BSL-3+ animal holding area, where they are housed in cages contained in bioclean portable laminar flow clean room enclosures (Lab Products, Seaford, Del.). Prior to infection, baseline temperatures are measured twice daily for at least 3 days. Ferrets are anesthetized with ketamine (25 mg/kg), xylazine (2 mg/kg), and atropine (0.05 mg/kg) by the intramuscular route and infected intranasally (i.n.) with virus/mL in phosphate-buffered saline (PBS) delivered to the nostrils. Control animals are mock-infected with an equivalent dilution (1:30) of noninfectious allantoic fluid. Stapled Peptides are administered i.v. or i.p. one hour after virus infection. Temperatures are measured twice daily using either a rectal thermometer or a subcutaneous implantable temperature transponder (BioMedic Data Systems, Inc., Seaford, Del.) with pre-infection values averaged to obtain a baseline temperature for each ferret. The change in temperature (in degrees Celsius) is calculated at each time point for each animal. Clinical signs of sneezing (before anesthesia), inappetence, dyspnea, and level of activity are assessed. A scoring system is also used to assess the activity level, and based on the daily scores for each animal in a group a relative inactivity index will be calculated. Rectal temperature and activity scores are used to assess the severity of influenza infection and the ability of Stapled Peptides to prevent flu symptoms
Assaying Inhibition of Viral Polymerase Complex Assembly and Activity
The technique of Bimolecular Fluorescence Complementation (“BiFC”) can be used to assay the compounds. In this technique, N- and C-terminal fragments of fluorescent proteins (e.g. GFP or its derivatives) are fused to interacting proteins. The two non-functional halves of the fluorophore, following the expression in cells, are brought into close proximity as a result of the specific protein interactions, which initiates folding of the fragments into an active protein and results in a detectable fluorescent signal at the site of the protein-protein complex. Thus, through BiFC, the specific interaction between PB1 and PA subunits can be visualized, quantified and localized within live cells. By disrupting PB1-PA interaction with a compound, the BiFC signal will be reduced, indicative of the presence of potential inhibitors targeting the assembly of PB1-PA complex. See Hemerka et. al., J. Virol. 2009, 3944-3955.

Manufacturing of the Aqueous Pharmaceutical Formulation

In another aspect of the disclosure relates to a method of making the aqueous pharmaceutical formulations disclosed herein. The method comprising the steps of dissolving at least one peptidomimetic macrocycle, or a pharmaceutically acceptable salt thereof in an aqueous solution. The method can further comprise of stirring the peptide mixture for some additional time. For example, the peptide mixture can be allowed to be stirred for an additional period of 1 min, 2 min, 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 15 min, 30 min, 45 min, 1 h, 1.5 h, 2 h or more.
In some embodiments, the peptidomimetic macrocycle is added to the aqueous solution at once. In some embodiments, the peptidomimetic macrocycle is added slowly to the aqueous solution, for example over a period of at least about 1 min, 2 min, 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 15 min, 30 min, 45 min, 1 h, 1.5 h, 2 h or more. In some embodiments, the peptidomimetic macrocycle is added slowly over a period of at most about 2 h, 1.5 h, 1 h, 45 min, 30 min, 15 min, 10 min, 9 min, 8 min, 7 min, 6 min, 5 min, 4 min, 3 min, 2 min, 1 min or less.
The aqueous solution comprises an aqueous diluent. The amount of the aqueous diluent can be in the range of about 10-99% w/v of formulation. In some embodiments, the amount of aqueous diluent in the formulation is about 50-99% w/v. For example, the amount of aqueous diluent is in the range of about 50-95% w/v, about 50-90% w/v, about 50-85% w/v, about 50-80% w/v, about 50-75% w/v, about 50-70% w/v, about 50-65% w/v, about 50-60% w/v, about 50-55% w/v, about 55-95% w/v, about 55-90% w/v, about 55-85% w/v, about 55-80% w/v, about 55-75% w/v, about 55-70% w/v, about 55-65% w/v, about 55-60% w/v, about 60-95% w/v, about 60-90% w/v, about 60-85% w/v, about 60-80% w/v, about 60-75% w/v, about 60-70% w/v, about 60-65% w/v, about 65-95% w/v, about 65-90% w/v, about 65-85% w/v, about 65-80% w/v, about 65-75% w/v, about 65-70% w/v, about 70-95% w/v, about 70-90% w/v, about 70-85% w/v, 70-80% w/v, about 70-75% w/v, about 75-95% w/v, about 75-90% w/v, about 75-85% w/v, about 75-80% w/v, about 70-95% w/v, about 70-90% w/v, about 70-85% w/v, about 70-80% w/v, about 70-75% w/v, about 75-95% w/v, about 75-90% w/v, about 75-85% w/v, about 75-80% w/v, about 80-95% w/v, about 80-90% w/v, about 80-85% w/v, about 85-95% w/v, about 85-90% w/v, or about 90-95% w/v. In some embodiments, the amount of aqueous diluent in a formulation is about 85-99% w/v. In some embodiments, the amount of the aqueous diluent in a formulation is about 85% w/v, about 86% w/v, about 87% w/v, about 88% w/v, about 89% w/v, about 90% w/v, about 91% w/v, about 92% w/v, about 93% w/v, about 94% w/v, about 95% w/v, about 96% w/v, about 97% w/v, about 98% w/v, or about 99% w/v. In some embodiments, the amount of the aqueous diluent in the formulation is about 90% w/v. In some embodiments, the amount of the diluent in the formulation is about 10% w/v, about 20% w/v, about 30% w/v, about 40% w/v, about 50% w/v, about 60% w/v, about 70% w/v, about 80% w/v, or about 90% w/v. In some embodiments the diluent is water (for example, water for injection) and it comprises about 90% w/v of the formulation.
The amount of a peptidomimetic macrocycle in the aqueous pharmaceutical formulations disclosed herein can range from about 0.0001-50.0% w/v. For example, the amount of the a peptidomimetic macrocycle can be about 0.0001-10.0 w/v %, about 0.005-10.0% w/v, about 0.01-10.0% w/v, about 0.05-10.0% w/v, about 0.1-10.0% w/v, about 0.5-10.0% w/v, about 1.0-10.0% w/v, about 2.0-10.0% w/v, about 3.0-10.0% w/v, about 4.0-10.0% w/v, about 5.0-10.0% w/v, 6.0-10.0% w/v, about 7.0-10.0% w/v, about 8.0-10.0% w/v, about 9.0-10.0% w/v, about 0.0001-5.0 w/v %, about 0.005-5.0% w/v, about 0.01-5.0% w/v, about 0.05-5.0% w/v, about 0.1-5.0% w/v, about 0.5-5.0% w/v, about 1.0-5.0% w/v, about 2.0-5.0% w/v, about 3.0-5.0% w/v, about 4.0-5.0% w/v, about 0.0001-2.0 w/v %, about 0.005-2.0% w/v, about 0.01-2.0% w/v, about 0.05-2.0% w/v, about 0.1-2.0% w/v, about 0.5-2.0% w/v, or about 1.0-2.0% w/v. In some embodiments, the peptidomimetic macrocycle is a p53-based peptidomimetic macrocycle and the amount is about 0.1-5.0% w/v, for example about 1.0% w/v, about 1.5% w/v, or about 2.0% w/v.
In some embodiments, the amount of the peptidomimetic macrocycle is in the range of about 1-20.0% w/v, 5-20.0% w/v, about 7-20.0% w/v, about 10-20.0% w/v, about 12-20.0% w/v, 15-20.0% w/v, 17-20.0% w/v, about 5-25.0% w/v, 7-25.0% w/v, 10-25.0% w/v, 12-25.0% w/v, 15-25.0% w/v, 17-25.0% w/v, 20-25.0% w/v, or 22-25.0% w/v; 5-35.0% w/v, 7-35.0% w/v, 10-35.0% w/v, 12-35.0% w/v, 15-35.0% w/v, 17-35.0% w/v, 20-35.0% w/v, 22-35.0% w/v, 25-35.0% w/v, 27-35.0% w/v, 30-35.0% w/v, or 32-35.0% w/v; 5-40.0% w/v, 7-40.0% w/v, about 10-40.0% w/v, about 12-40.0% w/v, about 15-40.0% w/v, about 17-40.0% w/v, about 20-40.0% w/v, 22-40.0% w/v, 25-40.0% w/v, 27-40.0% w/v, 30-40.0% w/v, 33-40.0% w/v, 35-40.0% w/v, or 37-40.0% w/v; 5-50.0% w/v, 10-50.0% w/v, 12-50.0% w/v, 15-50.0% w/v, 20-50.0% w/v, 22-50.0% w/v, 25-50.0% w/v, 27-50.0% w/v, 30-50.0% w/v, 32-50.0% w/v, 35-50.0% w/v, 37-50.0% w/v, 40-50.0% w/v, 42-50.0% w/v, 45-50.0% w/v, or 47-50.0% w/v.
In some embodiments, the amount of peptidomimetic macrocycle is about 0.5%, about 1.0%, about 1.5%, about 2.0%, about 2.5%, about 3.0%, about 3.5%, about 4.0%, about 4.5%, about 5.0%, about 5.5%, about 6.0%, about 6.5%, about 7.0%, about 7.5%, about 8.0%, about 8.5%, about 9.0%, about 9.5, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, or about 50% w/v.
The concentration of a peptidomimetic macrocycle in the aqueous pharmaceutical formulations disclosed herein can be in the range of about 1-100 mg/mL. In some embodiments, the amount of a peptidomimetic macrocycle in the formulation is about 1-5 mg/mL, about 1-10 mg/mL, about 1-15 mg/mL, about 1-20 mg/mL, about 1-25 mg/mL, about 1-30 mg/mL, about 1-35 mg/mL, about 1-40 mg/mL, about 1-45 mg/mL, about 1-50 mg/mL, about 1-60 mg/mL, about 1-70 mg/mL, about 1-80 mg/mL, about 1-90 mg/mL, about 5-10 mg/mL, about 5-15 mg/mL, about 5-20 mg/mL, about 5-25 mg/mL, about 5-30 mg/mL, about 5-35 mg/mL, about 5-40 mg/mL, about 5-45 mg/mL, about 5-50 mg/mL, about 5-60 mg/mL, about 5-70 mg/mL, about 5-80 mg/mL, about 5-90 mg/mL, about 5-100 mg/mL, about 10-15 mg/mL, about 10-20 mg/mL, about 10-25 mg/mL, about 10-30 mg/mL, about 10-35 mg/mL, about 10-40 mg/mL, about 10-45 mg/mL, about 10-50 mg/mL, about 10-60 mg/mL, about 10-70 mg/mL, about 10-80 mg/mL, about 10-90 mg/mL, about 10-100 mg/mL, about 15-20 mg/mL, about 15-25 mg/mL, about 15-30 mg/mL, about 15-35 mg/mL, about 15-40 mg/mL, about 15-45 mg/mL, about 15-50 mg/mL, about 15-60 mg/mL, about 15-70 mg/mL, about 15-80 mg/mL, about 15-90 mg/mL, about 15-100 mg/mL, about 20-25 mg/mL, about 20-30 mg/mL, about 20-35 mg/mL, about 20-40 mg/mL, about 20-45 mg/mL, about 20-50 mg/mL, about 20-60 mg/mL, about 20-70 mg/mL, about 20-80 mg/mL, about 20-90 mg/mL, about 20-100 mg/mL, about 25-30 mg/mL, about 25-35 mg/mL, about 25-40 mg/mL, about 25-45 mg/mL, about 25-50 mg/mL, about 25-60 mg/mL, about 25-70 mg/mL, about 25-80 mg/mL, about 25-90 mg/mL, about 25-100 mg/mL, about 30-35 mg/mL, about 30-40 mg/mL, about 30-45 mg/mL, about 30-50 mg/mL, about 30-60 mg/mL, about 30-70 mg/mL, about 30-80 mg/mL, about 30-90 mg/mL, about 30-100 mg/mL, about 35-40 mg/mL, about 35-45 mg/mL, about 35-50 mg/mL, about 35-60 mg/mL, about 35-70 mg/mL, about 35-80 mg/mL, about 35-90 mg/mL, about 35-100 mg/mL, about 40-45 mg/mL, about 40-50 mg/mL, about 40-60 mg/mL, about 40-70 mg/mL, about 40-80 mg/mL, about 40-90 mg/mL, about 45-50 mg/mL, about 45-60 mg/mL, about 45-70 mg/mL, about 45-80 mg/mL, about 45-90 mg/mL, about 40-100 mg/mL, about 50-60 mg/mL, about 50-70 mg/mL, about 50-80 mg/mL, about 50-90 mg/mL, about 50-100 mg/mL, about 60-70 mg/mL, about 60-80 mg/mL, about 60-90 mg/mL, about 60-100 mg/mL, about 70-80 mg/mL, about 70-90 mg/mL, about 70-100 mg/mL, about 80-90 mg/mL, about 80-100 mg/mL or about 90-100 mg/mL. In some embodiments, the amount of the peptidomimetic macrocycles in the formulations of the disclosure can be about 1 mg/mL, about 2 mg/mL, about 3 mg/mL, about 4 mg/mL, about 5 mg/mL, about 6 mg/mL, about 7 mg/mL, about 8 mg/mL, about 9 mg/mL, about 10 mg/mL, about 11 mg/mL, about 12 mg/mL, about 13 mg/mL, about 14 mg/mL, about 15 mg/mL, about 16 mg/mL, about 17 mg/mL, about 18 mg/mL, about 19 mg/mL, or about 20 mg/mL. In some embodiments, the amount of the peptidomimetic macrocycles is about 5 mg/mL, about 10 mg/mL, about 15 mg/mL or about 20 mg/mL. In some embodiments, the peptidomimetic macrocycle is a p53-based peptidomimetic macrocycle and the amount is about 1-20 mg/mL, for example about 1.0 mg/mL, about 5 mg/mL, about 10 mg/mL, about 15 mg/mL or about 20 mg/mL.
In some embodiments, the aqueous solution further comprises a buffering agent. In such embodiments, the method of making the aqueous pharmaceutical formulations disclosed herein comprises dissolving at least one buffering agent in the aqueous diluent, and adding at least one peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof. In some embodiments, the peptidomimetic macrocycle is added at once. In some embodiments, the peptidomimetic macrocycle is added slowly over a period of time as described above. As described above, the method can further comprise of stirring the peptide mixture for some additional time.
The concentration of the buffering solution can be about 0.01-100 mM. In some embodiments the concentration of the buffering solution is at least 0.1 mM, 1 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, 60 mM, 70 mM, 80 mM, 90 mM, 100 mM. In some embodiments the concentration of the buffering solution is at most 0.1 mM, 1 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, 60 mM, 70 mM, 80 mM, 90 mM, 100 mM. In some embodiments, the concentration of the buffering agent is about 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM, 10 mM, 11 mM, 12 mM, 13 mM, 14 mM, 15 mM, 16 mM, 17 mM, 18 mM, 19 mM, 20 mM, 21 mM, 22 mM, 23 mM, 24 mM, 25 mM, 26 mM, 27 mM, 28 mM, 29 mM, 30 mM, 31 mM, 32 mM, 33 mM, 34 mM, 35 mM, 36 mM, 37 mM, 38 mM, 39 mM, 40 mM, 41 mM, 42 mM, 43 mM, 44 mM, 45 mM, 46 mM, 47 mM, 48 mM, 49 mM, 50 mM, 60 mM, 70 mM, 80 mM, 90 mM, or 100 mM.
The method can further involve maintaining the pH of the formulation. For example, maintaining the pH of the reaction medium while the peptidomimetic macrocycle is being added and/or dissolved therein. The pH can be maintained by the addition of a pH adjusting agent. Any suitable pH adjusting agents as described above and throughout the disclosure can be used.
Non-limiting examples of suitable pH adjusting agents which can be included in the methods disclosed herein are hydrochloric acid, sodium hydroxide, citric acid, phosphoric acid, lactic acid, tartaric acid, succinic acid, or mixtures thereof. In one embodiment, the pH adjusting agent is hydrochloric acid. In one embodiment, the pH adjusting agent is sodium hydroxide. In one embodiment, the pH adjusting agent is phosphoric acid. In one embodiment, the pH adjusting agent is lactic acid. In one embodiment, the pH adjusting agent is tartaric acid. In one embodiment, the pH adjusting agent is tartaric acid. In one embodiment, the pH adjusting agent is succinic acid. In one embodiment, the buffering agent is a phosphate buffer and the pH adjusting agent in sodium hydroxide. For example, the buffering agent can be NaH₂PO₄and the pH adjusting agent can be sodium hydroxide, or the buffering agent can be Na₂HPO₄and the pH adjusting agent can be sodium hydroxide, other buffering agent can be a mixture of NaH₂PO₄and Na₂HPO₄and the pH adjusting agent can be sodium hydroxide, or buffering agent can be KH₂PO₄and the pH adjusting agent can be sodium hydroxide, or the buffering agent can be K₂HPO₄and the pH adjusting agent can be sodium hydroxide, or the buffering agent can be a mixture of KH₂PO₄and K₂HPO₄and the pH adjusting agent can be sodium hydroxide.
In some embodiments the amount of the pH adjusting agent added to the aqueous pharmaceutical formulation is in the range of about 0.001-1% w/v. For example, in some embodiments, the amount of the pH adjusting agent present is in the range of 0.01-0.1% w/v, 0.1-1% w/v, 0.005-1% w/v, 0.05-1% w/v, 0.5-1% w/v, 0.001-0.5% w/v, 0.01-0.5% w/v, 0.1-0.5% w/v, 0.001-0.1% w/v, or 0.01-0.1 vv. In some embodiments, the amount of the pH adjusting agent present in the formulation is in the range of about 0.01-0.1% w/v. In some embodiments, the amount of the pH adjusting agent present in the formulation is at least 0.010% w/v, 0.02% w/v, 0.03% w/v, 0.04% w/v, 0.05% w/v, 0.06% w/v, 0.07% w/v, 0.08% w/v, 0.09% w/v, or 0.1% w/v. In some embodiments, the amount of the pH adjusting agent present in the formulation is at most 0.1% w/v, 0.09% w/v, 0.08% w/v, 0.07% w/v, 0.06% w/v, 0.05% w/v, 0.04% w/v, 0.03% w/v, 0.02% w/v, 0.01% w/v.
In some embodiments the amount of the pH adjusting agent added to the aqueous pharmaceutical formulation is in the range of about 0.01-100 mg/mL. For example, in some embodiments, the amount of the pH adjusting agent present is in the range of 0.01-50 mg/mL, 0.01-10 mg/mL, 0.1-100 mg/mL, 0.1-50 mg/mL, 0.1-10 mg/mL, 1-100 mg/mL, 1-50 mg/mL, or 1-10 mg/mL. In some embodiments, the amount of the pH adjusting agent present in the formulation is in the range of about 1-10 mg/mL. In some embodiments, the amount of the pH adjusting agent present in the formulation is at least 1 mg/mL, 2 mg/mL, 3 mg/mL, 4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL, 8 mg/mL, 9 mg/mL, or 10 mg/mL. In some embodiments, the amount of the pH adjusting agent present in the formulation is at most 10 mg/mL, 9 mg/mL, 8 mg/mL, 7 mg/mL, 6 mg/mL, 5 mg/mL, 4 mg/mL, 3 mg/mL, 2 mg/mL, 1 mg/mL. In some embodiments, the amount of the pH adjusting agent present in the formulation is about 1 mg/mL, about 1.5 mg/mL, about 2 mg/mL, about 2.5 mg/mL, about 3 mg/mL, about 3.5 mg/mL, about 4 mg/mL, about 4.5 mg/mL, about 5 mg/mL, about 6 mg/mL, about 7 mg/mL, about 8 mg/mL, about 9 mg/mL, about 10 mg/mL, about 12 mg/mL, about 14 mg/mL, about 16 mg/mL, about 18 mg/mL, or about 20 mg/mL. In some embodiments, the amount of the pH adjusting agent present in the formulation is of the pH adjusting agent is present in about 5 mg/mL of the formulation.
In some embodiments, the aqueous solution comprises a stabilizing agent. In such embodiments, the method of making the aqueous pharmaceutical formulations disclosed herein comprises dissolving at least one stabilizing agent in at least an aqueous diluent, and adding at least one peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof. In some embodiments, the peptidomimetic macrocycle is added at once. In some embodiments, the peptidomimetic macrocycle is added slowly over a period of time as described above. As described above, the method can further comprise of stirring the peptide mixture for some additional time.
In amount of the stabilizing agent in the formulations can be in the range of about 0.001-1% w/v. For example, in the range of about 0.001-0.01%, about 0.001-0.1% w/v, about 0.001-0.5% w/v, about 0.01-0.1% w/v, about 0.01-0.5% w/v, about 0.01-0.1% w/v, about 0.1-0.5% w/v or about 0.5-1% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 00.01-0.1% w/v. In some embodiments, the amount of the stabilizing agent is at least about 0.01% w/v, about 0.02% w/v, about 0.03% w/v, about 0.04% w/v, about 0.05% w/v, about 0.06% w/v, about 0.07% w/v, about 0.08% w/v, about 0.09% w/v, or about 0.1% w/v. In some embodiments, the amount of the stabilizing agent is at most about 0.10% w/v, about 0.09% w/v, about 0.08% w/v, about 0.07% w/v, about 0.06% w/v, about 0.05% w/v, about 0.04% w/v, about 0.03% w/v, about 0.02% w/v, about 0.01% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.01% w/v, about 0.02% w/v, about 0.03% w/v, about 0.04% w/v, about 0.05% w/v, about 0.06% w/v, about 0.07% w/v, about 0.08% w/v, about 0.09% w/v, or about 0.1% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.01% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.02% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.03% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.04% w/v. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.05% w/v.
In some embodiments the amount of the stabilizing agent is about 0.01-10 mg/mL. For example, in some embodiments, the amount of the stabilizing agent is about 0.01-5 mg/mL, about 0.01-1 mg/mL, about 0.01-0.5 mg/mL, about 0.01-0.1 mg/mL, about 0.1-10 mg/mL, about 0.1-5 mg/mL, about 0.1-1 mg/mL, about 0.1-0.5 mg/mL, about 1-10 mg/mL, or about 1-5 mg/mL. In some embodiments, the amount of the stabilizing agent in the formulation is in the range of about 0.01-1.0 mg/mL.
In some embodiments, the amount of the stabilizing agent is at least about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 0.6 mg/mL, about 0.7 mg/mL, about 0.8% mg/mL, about 0.9 mg/mL, or about 1 mg/mL. In some embodiments, the amount of the stabilizing agent is at most about 1 mg/mL, about 0.9 mg/mL, about 0.8 mg/mL, about 0.7 mg/mL, about 0.6 mg/mL, about 0.5 mg/mL, about 0.4 mg/mL, about 0.3 mg/mL, about 0.2 mg/mL, or about 0.1 mg/mL.
In some embodiments, the amount of the stabilizing agent is about 0.1 mg/mL, about 0.2 mg/mL, about 0.3 mg/mL, about 0.4 mg/mL, about 0.5 mg/mL, about 0.6 mg/mL, about 0.7 mg/mL, about 0.8 mg/mL, about 0.9 mg/mL, or about 1 mg/mL. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.1 mg/mL. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.2 mg/mL. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.3 mg/mL. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.4 mg/mL. In some embodiments, the amount of the stabilizing agent in the formulation is about 0.5 mg/mL.
In some embodiments, the aqueous solution comprises both a buffering agent and stabilizing agent. In such embodiments, the method of making the aqueous pharmaceutical formulations disclosed herein comprises dissolving at least one stabilizing agent and at least one buffering agent in an aqueous diluent, and adding at least one peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof. In some embodiments, the peptidomimetic macrocycle is added at once. In some embodiments, the peptidomimetic macrocycle is added slowly over a period of time as described above. As described above, the method can further comprise of stirring the peptide mixture for some additional time.
In some examples, the method of making the aqueous pharmaceutical formulations disclosed herein comprises dissolving at least one buffering agent, at least one tonicity adjusting agent and at least one stabilizing agent in at least one aqueous diluent, and adding at least one peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof. In some embodiments, the buffering agent, the tonicity adjusting agent and the stabilizing agents are dissolved in the aqueous diluent in this order. In some embodiments, the peptidomimetic macrocycle is added at once. In some embodiments, the peptidomimetic macrocycle is added slowly over a period of time as described above.
The amount of the tonicity adjusting agent in the aqueous pharmaceutical formulations disclosed herein can be in the range of about 0.001-50% w/v, for example about 0.001-0.1% w/v, about 0.001-1.0% w/v, about 0.001-10% w/v, about 1-10% w/v, about 1-20% w/v, about 1-30% w/v, about 1-40% w/v, about 1-50% w/v, about 5-10% w/v, about 5-20% w/v, about 5-30% w/v, about 5-40% w/v, about 5-50% w/v, about 10-20% w/v, about 10-30% w/v, about 10-40% w/v, about 10-50% w/v, about 15-20% w/v, about 15-30% w/v, about 15-40% w/v, about 15-50% w/v, about 20-30% w/v, about 20-40% w/v, about 20-50% w/v, about 25-30% w/v, about 25-40% w/v, about 25-50% w/v, about 30-40% w/v, about 30-50,% w/v, about 35-40% w/v, about 35-50% w/v, about 40-50% w/v, or about 45-50% w/v. In some embodiments, the amount of the tonicity adjusting agent is about 1% w/v, about 2% w/v, about 3% w/v, about 4% w/v, about 5% w/v, about 6% w/v, about 7% w/v, about 8% w/v, about 9% w/v, or about 10% w/v. In some embodiments, the amount of the tonicity adjusting agent is about 7% w/v. In some embodiments, the amount of the tonicity adjusting agent is about 8% w/v. In some embodiments, the amount of the tonicity adjusting agent is about 9% w/v. In some embodiments, the amount of the tonicity adjusting agent is about 10% w/v.
The concentration of the tonicity adjusting agent can vary in the range of about 1-500 mg/mL. For example, the concentration of the tonicity adjusting agent in the aqueous pharmaceutical formulations disclosed herein can be in the range of about 1-400 mg/mL, 1-300 mg/mL, 1-200 mg/mL, 1-100 mg/mL, 10-500 mg/mL, 10-400 mg/mL, 10-300 mg/mL, 10-200 mg/mL, 10-100 mg/mL, 20-500 mg/mL, 20-400 mg/mL, 20-300 mg/mL, 20-200 mg/mL, 20-100 mg/mL, 30-500 mg/mL, 30-400 mg/mL, 30-300 mg/mL, 30-200 mg/mL, 30-100 mg/mL, 40-500 mg/mL, 40-400 mg/mL, 40-300 mg/mL, 40-200 mg/mL, 40-100, mg, 50-500 mg/mL, 50-400 mg/mL, 50-300 mg/mL, 50-200 mg/mL, 50-100 mg/mL, 60-500 mg/mL, 60-400 mg/mL, 60-30 mg/mL, 60-200 mg/mL, 60-100 mg/mL, 70-500 mg/mL, 70-400 mg/mL, 70-300 mg/mL, 70-200 mg/mL, 70-100 mg/mL, 80-500 mg/mL, 80-400 mg/mL, 80-300 mg/mL, 80-200 mg/mL, 80-200 mg/mL, 90-500 mg/mL, 90-400 mg/mL, 90-300 mg/mL, 90-200 mg/mL, 90-100 mg/mL, 100-500 mg/mL, 100-400 mg/mL, 100-300 mg/mL, 100-200 mg/mL, 200-500 mg/mL, 200-400 mg/mL, 200-300 mg/mL, 300-500 mg/mL, 300-400 mg/mL or 400-500 mg/mL. In some embodiments, the concentration of the tonicity adjusting agent is about 10 mg/mL, about 20 mg/mL, about 30 mg/mL, about 40 mg/mL, about 50 mg/mL, about 60 mg/mL, about 70 mg/mL, about 80 mg/mL, about 90 mg/mL, or about 100 mg/mL. In some embodiments, the concentration of the tonicity adjusting agent is about 50 mg/mL. In some embodiments, the concentration of the tonicity adjusting agent is about 80 mg/mL. In some embodiments, the concentration of the tonicity adjusting agent is about 100 mg/mL. In some embodiments, the tonicity adjusting agent is trehalose (for example, D-trehalose) and the concentration is about 80 mg/mL.
In some embodiments, the concentration of the tonicity adjusting agent is between about 100-500 mM. For example the concentration of the tonicity adjusting agent in the aqueous pharmaceutical formulations disclosed herein can be 100-400 mM, 100-300 mM, 100-200 mM, 200-500 mM, 200-400 mM, 200-300 mM, 300-500 mM, 300-400 mM or 400-500 mM. In some embodiments, the concentration of the tonicity adjusting agent is between about 200-300 mM, for example 210-300 mM, 220-300 mM, 230-300 mM, 240-300 mM, 250-300 mM, 260-300 mM, 270-300 mM, 280-300 mM, 290-300 mM, 200-290 mM, 210-290 mM, 220-290 mM, 230-290 mM, 240-290 mM, 250-290 mM, 260-290 mM, 270-290 mM, 280-290 mM, 200-280 mM, 210-280 mM, 220-280 mM, 230-280 mM, 240-280 mM, 250-280 mM, 260-280 mM, 270-280 mM, 200-270 mM, 210-270 mM, 220-270 mM, 230-270 mM, 240-270 mM, 250-270 mM, 260-270 mM, 200-260 mM, 210-260 mM, 220-260 mM, 230-260 mM, 240-260 mM, 250-260 mM, 200-250 mM, 210-250 mM, 220-250 mM, 230-250 mM, 240-250 mM, 200-240 mM, 210-240 mM, 220-240 mM, 230-240 mM, 200-230 mM, 210-230 mM, 220-230 mM, 200-220 mM, 210-220 mM, or 210-220 mM. In some embodiments, the concentration of the tonicity adjusting agent is between about 220-260 mM. For example, about 220 mM, 230 mM, 240 mM, 250 mM, or 260 mM.
The methods described herein can additionally comprise addition of one or more optional excipients and/or ingredients. For example addition of one or more antioxidants, antimicrobial agent, surfactants, lubricants, thickening agents, preservatives, chelating agents.
In some embodiments the amount of antioxidants used is in the range of about 0.001-5% w/v, for example about 0.001-4.5%, 0.001-4%, 0.001-3%, 0.001-2%, 0.002-1%, 0.001-0.5%, or 0.001-0.05% w/v. In some embodiments the amount of antioxidants used is in the range of about 0.001-about 0.5%, about 0.1-about 0.5%, about 0.2-about 0.5%, about 0.3-about 0.5%, about 0.4-about 0.5%, about 0.01-about 0.4%, about 0.1-about 0.4%, about 0.2-about 0.4%, about 0.3-about 0.4%, about 0.01-about 0.3%, about 0.1-about 0.3%, about 0.2-about 0.3%, about 0.01-about 0.2%, about 0.1-about 0.2%, or about 0.01-about 0.1% w/v.
Such antimicrobial agents can be employed at a level of from about 0.005-0.5% w/v, for example about 0.001-0.01% w/v, about 0.01-0.1% w/v, about 0.1-0.5% w/v or about 0.01-0.05% w/v.
The methods described herein can additionally comprise prefiltering and/or clarifying the peptidomimetic formulation by a suitable process, for example by centrifugation or by filtration. Filtration can be by any suitable means, for example by depth filter media or by membrane filters. In some embodiments, filtration can be by means of a 0.22 micrometer filters.
The method can optionally involve sterilization of the aqueous pharmaceutical formulations. Sterilization can be performed by any suitable technique. For example, a suitable sterilization method can include one or more of sterile filtration, chemical, irradiation heat filtration, and addition of a chemical disinfectant to the aqueous pharmaceutical formulation. In some examples, the formulations are sterilized by moist heat sterilization. In some examples, the formulations are sterilized by dry heat sterilization. In some examples, the formulations are sterilized by chemical cold sterilization. In some examples, the formulations are sterilized by radiation sterilization. In some examples, the formulations are sterilized by filtration. In some examples, the formulations are sterilized by filtration using an appropriate micron sterilizing grade filters. The filtration can be carried out by any suitable means, e.g. cellulose-based filters, cellulosic esters (MCE), polyvinylidene fluoride (PVDF), polytetrafluoroethylene (PTFE), or polyethersulfone (PES) filters. In some embodiments PVDF filters are used. Filters of any appropriate micron size can be used. In some embodiments, the filter size can be 0.001-0.5 micrometer, for example 0.001-0.01 micrometer, 0.01-0.1 micrometer, 0.1-0.2 micrometer, 0.2-0.3 micrometer, 0.3-0.43 micrometer or 0.4-0.5 micrometer. In some embodiments 0.22 micrometer filters are used. In some embodiments 0.22 micrometer PVDF filters are used.
The aqueous pharmaceutical formulations can be in a form that is suitable for direct administration or can be in a concentrated form that requires dilution relative to what is administered to the patient. For example, aqueous pharmaceutical formulations, described in this disclosure, can be in a form that is suitable for direct administration without any further dilution or reconstitution. The formulations can be diluted or reconstituted prior to administration with a suitable aqueous diluent(s) to obtain a finished concentration. The diluent can be an injection or infusion fluid. Examples of injection or infusion fluid include, but are not limited to, WFI (Bacteriostatic Water For Injection), SWFI (Sterile Water For Injection), D5W (Dextrose 5% in Water), D;OW (Dextrose 10% in Water), D5LR (Dextrose in Lactate Ringer's Solution), D5%/S (Dextrose 5% in ¼ Strength Saline (5% Dextrose and 0.22% Sodium Chloride Injection)), D5½S (Dextrose 5% in ½ Strength Saline (5% Dextrose and 0.45% Sodium Chloride Injection)), D5NS (Dextrose 5% in Normal Saline (5% Dextrose and 0.9% Sodium Chloride Injection)), D5R (Dextrose 5% in Ringer's Injection), DIONS (Dextrose 10% in Normal Saline (10% Dextrose and 0.9% Sodium Chloride Injection)), ISlOW (Invert Sugar 10% in Saline (10% Invert Sugar in 0.9% Sodium Chloride Injection)), LR (Lactated Ringer's Injection), Pr (Protein Hydrolysate Injection), R (Ringer's Injection), NS Sodium Chloride 0.9% (Normal Saline), SOD CL 5 (Sodium Chloride 5% (5% Sodium Chloride Injection), and Sod Lac (Sodium Lactate, ⅙ Molar (M/6 Sodium Lactate Injection)). In some examples, the formulations can be diluted with 0.9% sodium chloride, 5% dextrose in water (D5W), 5% dextrose in normal saline (D5NS), 5 dextrose in half amount of normal saline (D5½NS), lactated ringer's injection or a mixture thereof. Dilution/reconstitution can be performed immediately prior to the administration. In some cases, dilution/reconstitution can be performed shortly before the administration. In some cases, the dilution is performed at most 1 min, 5 min, 15 min, 30 min, 45 min, 60 min, 90 min, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, 12 h, 13 h, 14 h, 15 h, 16 h, 17 h, 18 h, 19 h, 20 h, 21 h, 22 h, 23 h, or 24 h before the administration to the subject. In some examples the reconstituted and diluted solutions is used within 1-10 hours, 2-8 hours, 3-7 hours, 4-6 hours reconstitution and/or dilution. In some examples, the formulations are diluted/reconstituted more than 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 1 week before administration.
FIG. 1 depicts the manufacturing process of an exemplary aqueous formulation according to the disclosure.

Purity, Stability and Degradation

The formulations of the disclosure can be characterized by low endotoxin concentration. In some embodiments, the formulations can have a concentration of endotoxin of less than about 100 EU/mL, for example, less than about 90 EU/mL, 80 EU/mL, 70 EU/mL, 60 EU/mL, 50 EU/mL, 40 EU/mL, 30 EU/mL, 20 EU/mL, 10 EU/mL, 5 EU/mL, 1 EU/mL, 0.5 EU/mL, 0.2 EU/mL, 0.1 EU/mL, 0.05 EU/mL, 0.01 EU/mL, 0.005 EU/mL, or 0.001 EU/mL. In some embodiments the concentration of the endotoxin is 0.1-10 EU/mL, for example about 0.1-1 EU/mL, 0.1-2 EU/mL, 0.1-3 EU/mL, 0.1-4 EU/mL, 0.1-5 EU/mL, 0.1-6 EU/mL, 0.1-7 EU/mL, 0.1-8 EU/mL, 0.1-9 EU/mL, 1-2 EU/mL, 1-3 EU/mL, 1-4 EU/mL, 1-5 EU/mL, 1-6, 1-7, 1-8, 1-9, 1-10, 2-3 EU/mL, 2-4 EU/mL, 2-5 EU/mL, 2-6, 2-7, 2-8, 2-9, 2-10, 3-4 EU/mL, 3-5 EU/mL, 3-6 EU/mL, 3-7 EU/mL, 3-8 EU/mL, 3-9 EU/mL, 3-10 EU/mL, 4-5 EU/mL, 4-6 EU/mL, 4-7 EU/mL, 4-8 EU/mL, 4-9 EU/mL, 4-10 EU/mL, 5-6 EU/mL, 5-7 EU/mL, 5-8 EU/mL, 5-9 EU/mL, 5-10 EU/mL, 6-7 EU/mL, 6-8 EU/mL, 6-9 EU/mL, 6-10 EU/mL, 7-8 EU/mL, 7-9 EU/mL, 7-10 EU/mL, 8-9 EU/mL, 8-10 EU/mL, or 9-10 EU/mL.
In some embodiments the formulations of the disclosure are essentially particulate-free solutions. In some embodiments, the formulation is essentially free of particles of size greater than about 1 μm, 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 17 μm, 18 μm, 19 μm, 20 μm, 21 μm, 22 μm, 23 μm, 24 μm, 25 μm, 26 μm, 27 μm, 28 μm, 29 μm, 30 μm, 35 μm, 40 μm, 45 μm, 50 μm, or more.
In some embodiments, the formulation comprise at most about 1, about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9, about 10, about 11, about 12, about 13, about 14, about 15, about 16, about 17, about 18, about 19, about 20, about 21, about 22, about 23, about 24, about 25, about 26, about 27, about 28, about 29, about 30, about 50, about 100, about 200, about 300, about 400, about 500, about 600, about 700, about 800, about 900, about 1,000, about 1,100, about 1,200, about 1,300, about 1,400, about 1,500, about 1,600, about 1,700, about 1,800, about 1,900, about 2,000, about 2,200, about 2,400, about 2,600, about 2,800, about 3,000, about 3,500, about 4,000, about 4,500, about 5,000, about 5,500, about 6,000, about 6,500, about 7,000, about 8,000, about 8,500, about 9,000, about 9,500, or about 10,000 particles of size greater than or equal to 10 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations of the disclosure are essentially free of particles of size greater than or equal to 10 μm. In some embodiments the formulations of the disclosure less than 500 particles of size greater than or equal to 10 μm in per 1 mL or 5 mL of formulation. In some embodiments the formulations of the disclosure less than 1000 particles of size greater than or equal to 10 μm in per 1 mL or 5 mL of formulation. In some embodiments the formulations of the disclosure less than 1200 particles of size greater than or equal to 10 μm in per 1 mL or 5 mL of formulation. In some embodiments the formulations of the disclosure less than 1,000-1,200 particles of size greater than or equal to 10 μm in per 1 mL or 5 mL of formulation.
In some embodiments, the formulation comprise at most about 1, about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9, about 10, about 11, about 12, about 13, about 14, about 15, about 16, about 17, about 18, about 19, about 20, about 21, about 22, about 23, about 24, about 25, about 26, about 27, about 28, about 29, about 30, about 50, about 60, about 70, about 80, about 90, about 100, about 110, about 120, about 130, about 140, about 150, about 200, about 300, about 400, about 500, about 600, about 700, about 800, about 900, about 1,000, about 1,100, about 1,200, about 1,300, about 1,400, about 1,500, about 1,600, about 1,700, about 1,800, about 1,900, about 2,000, about 2,500, about 3,000, about 3,500, about 4,000, about 4,500, about 5,000, about 5,500, or about 6,000 particles of size greater than or equal to 25 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations of the disclosure are essentially free of particles of size greater than or equal to 25 μm. In some embodiments the formulations comprise at most 50 particles of size greater than or equal to 25 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations comprise at most 100 particles of size greater than or equal to 25 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations comprise at most 120 particles of size greater than or equal to 25 μm in per 1 mL or 5 mL of formulation. In some embodiments the formulations comprise about 100-120 particles of size greater than or equal to 25 μm per 1 mL or 5 mL of formulation.
In some embodiments the formulations of the disclosure are essentially free of particles of size greater than or equal to 50 μm. In some embodiments the formulations comprise at most 1 particles of size greater than or equal to 50 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations comprise at most 2 particles of size greater than or equal to 50 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations comprise at most 3 particles of size greater than or equal to 50 μm in per 1 mL or 5 mL of formulation. In some embodiments the formulations comprise about 1-5 particles of size greater than or equal to 50 μm per 1 mL or 5 mL of formulation. In some embodiments the formulations of the disclosure are essentially free of particles of size greater than or equal to 50 μm. In some embodiments the formulations comprise at most 1 particles of size greater than or equal to 50 μm per container. In some embodiments the formulations comprise at most 2 particles of size greater than or equal to 50 μm per container. In some embodiments the formulations comprise at most 3 particles of size greater than or equal to 50 μm in per container. In some embodiments the formulations comprise about 1-5 particles of size greater than or equal to 25 μm container.
In some embodiments, the formulations comprise 0-10000, 100-10,000, 500-10,000, 1,000-10,000, 1,500-10,000, 2,000-10,000, 2,500-10,000, 3,000-10,000, 3,500-10,000, 4,000-10,000, 4,500-10,000, 5,000-10,000, 5,500-10,000, 6,000-10,000, 6,500-10,000, 7,000-10,000, 7,500-10,000, 8,000-10,000, 8,500-10,000, 9,000-10,000, or 9,500-10,000 particles of size greater than or equal to 10 μm per mL of formulation.
In some embodiments, the formulations comprise 0-10000, 100-10,000, 500-10,000, 1,000-10,000, 1,500-10,000, 2,000-10,000, 2,500-10,000, 3,000-10,000, 3,500-10,000, 4,000-10,000, 4,500-10,000, 5,000-10,000, 5,500-10,000, 6,000-10,000, 6,500-10,000, 7,000-10,000, 7,500-10,000, 8,000-10,000, 8,500-10,000, 9,000-10,000, or 9,500-10,000 particles of size greater than or equal to 10 μm per 5 mL of formulation.
In some embodiments, the formulations comprise 0-10000, 100-10,000, 500-10,000, 1,000-10,000, 1,500-10,000, 2,000-10,000, 2,500-10,000, 3,000-10,000, 3,500-10,000, 4,000-10,000, 4,500-10,000, 5,000-10,000, 5,500-10,000, 6,000-10,000, 6,500-10,000, 7,000-10,000, 7,500-10,000, 8,000-10,000, 8,500-10,000, 9,000-10,000, or 9,500-10,000 particles of size greater than or equal to 25 μm per mL of formulation.
In some embodiments, the formulations comprise 0-10000, 100-10,000, 500-10,000, 1,000-10,000, 1,500-10,000, 2,000-10,000, 2,500-10,000, 3,000-10,000, 3,500-10,000, 4,000-10,000, 4,500-10,000, 5,000-10,000, 5,500-10,000, 6,000-10,000, 6,500-10,000, 7,000-10,000, 7,500-10,000, 8,000-10,000, 8,500-10,000, 9,000-10,000, or 9,500-10,000 particles of size greater than or equal to 25 μm per 5 mL of formulation.
In some embodiments, the formulations comprise 0-10000, 100-10,000, 500-10,000, 1,000-10,000, 1,500-10,000, 2,000-10,000, 2,500-10,000, 3,000-10,000, 3,500-10,000, 4,000-10,000, 4,500-10,000, 5,000-10,000, 5,500-10,000, 6,000-10,000, 6,500-10,000, 7,000-10,000, 7,500-10,000, 8,000-10,000, 8,500-10,000, 9,000-10,000, or 9,500-10,000 particles of size greater than or equal to 50 μm per 1 mL of formulation
In some embodiments, the formulations comprise 0-10000, 100-10,000, 500-10,000, 1,000-10,000, 1,500-10,000, 2,000-10,000, 2,500-10,000, 3,000-10,000, 3,500-10,000, 4,000-10,000, 4,500-10,000, 5,000-10,000, 5,500-10,000, 6,000-10,000, 6,500-10,000, 7,000-10,000, 7,500-10,000, 8,000-10,000, 8,500-10,000, 9,000-10,000, or 9,500-10,000 particles of size greater than or equal to 50 μm per 5 mL of formulation.
In some embodiments, the formulations of the present disclosure can remain stable after exposure to a single or multiple freeze-thaw events. Formulations of the present disclosure can also remain stable after exposure to physical agitation, such as one would expect to encounter upon shipping product from one location to another. Stability can be measured by any one of a number of different ways, including visual inspection for precipitate formation, analysis of percent peptidomimetic macrocycle remaining in solution after exposure to stress conditions (e.g., by size-exclusion HPLC), or analysis of the formation of chemical variants and/or decomposition products of the peptidomimetic macrocycle (e.g., by anion exchange or reverse phase HPLC analysis). In some embodiments of the present disclosure, no precipitate visible to the naked eye is formed in the formulation after at least one freeze thaw event. In some embodiments the formulation remains stable after at least three freeze thaw events. In some embodiments the formulation remains stable after at least six freeze thaw events. In some embodiments, at least 80, 85, 90%, 95%, 96%, 975, 98%, or 99% of the peptidomimetic macrocycle remains in the formulation after at least one freeze thaw event.
In some embodiments, the total peptidomimetic degradation products formed in the formulations of the present disclosure is less than 1.0% when stored at a temperature of 40° C. for a period of one month. In some further embodiments, the total degradation products of the compound of Formula 1 formed is less than about 0.9%, about 0.8%, about 0.7%, about 0.6%, about 0.5%, about 0.4%, about 0.3%, about 0.2%, or about 0.1% when stored at a temperature of 40° C. for a period of one month.
In some embodiments, the total peptidomimetic degradation products formed in the formulations of the present disclosure is less than 1.0% when stored at a temperature of 40° C. for a period of about two months, about three months, about four months, about five months about six months.
In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 0.001%, 0.01%, 0.1%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, 4.5%, 5.0%, 5.5%, 6.0%, 6.5%, 7.0%, 7.5%, 8.0%, 8.5%, 9.0%, 9.5%, or 10%. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1.0%. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.0%. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 3.0%.
In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 4.0%. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 5.0%.
In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 0.5% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1.5% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.0% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.5% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 3.0% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 5.0% when stored at a temperature of −20° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months.
In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 0.5% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1.5% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.0% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.5% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 3.0% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 5.0% when stored at a temperature of 5° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months.
In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 0.5% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1.5% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.0% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.5% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 3.0% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 5.0% when stored at a temperature of 25° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months.
In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 0.5% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 1.5% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.0% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 2.5% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 3.0% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months. In some further embodiments, the amount of any single impurity in the formulation at any storage temperature is less than 5.0% when stored at a temperature of 40° C. for a period of 0 months, 0.5 months, 1.0 months, 1.5 months, 2.0 months, 2.5 months, 3.0 months, 3.5 months, 4.0 months, 4.5 months, 5.0 months, 5.5 months, 6.0 months, 8 months, 10 months, 12 months, or more months.
In some cases the pharmaceutically acceptable formulation expires in about 1-5 years. In some cases the formulation expires in about 1, 2, 3 or 4 years. In some cases the formulation expires in more than 5 years. In some cases the formulation expires in less than a year. In some cases the formulation expires in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11 months.
In some cases the total amount of peptidomimetic degradation products at the time of product expiration are in the range of above 0.1-10%. In some cases the total degradation product at the time of expiration is in the range of about 0.01-1, about 0.01-2, about 0.01-3, about 0.01-4, about 0.01-5, about 0.01-6, about 0.01-7, about 0.01-8, or about 0.01-9, about 1-2, about 1-3, about 1-4, about 1-5, about 1-6, about 1-7, about 1-8, about 1-9, about 2-3, about 3-4, about 2-5, about 2-6, about 2-7, about 2-8, about 2-9, about 3-4, about 3-5, about 3-6, about 3-7, about 3-8, about 3-9, about 3-10, about 4-5, about 4-6, about 4-7, about 4-8, about 4-9, about 4-10, about 5-6, about 5-7, about 5-8, about 5-9, about 5-10, about 6-7, about 6-8, about 6-9, about 6-10, about 7-8, about 7-9, about 7-10, about 8-9, about 8-10 or about 9-10%. In some embodiments the amount of total degradation product at the time of expiration is about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%. In some embodiments the amount of total degradation product at the time of expiration is about 0.01%, about 0.05%, about 0.1%, about 0.15%, about 0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.40%, about 0.45%, about 0.50%, about 0.55%, about 0.60%, about 0.65%, about 0.70%, about 0.75%, about 0.80%, about 0.85%, about 0.90%, about 0.95%, or about 1.0%.
In some cases aqueous pharmaceutical formulations of the instant disclosure are stored at −40 to 65° C., for example from −5 to 40° C. In some cases the formulations can be stored at about −40° C., about −30° C., −20° C., −10° C., −5° C., 0° C., about 5° C., about 10° C., about 15° C., about 20° C., about 25° C., about 30° C., about 35° C., about 40° C., about 45° C., about 50° C., about 55° C., about 60° C., or about 65° C. In some embodiments, the formulations are stored at or below ambient temperature. In some embodiments, the formulations are stored above ambient temperature.

Sparging

In some embodiments the stability of the peptidomimetic macrocycles in the formulations of the disclosure can be improved by sparging the formulation with an inert gas. A variety of inert gases can be used as a sparging material including but not limited to nitrogen, argon, helium, or a combination thereof. In some embodiments the inert gas is nitrogen. The sparging is generally carried out till the oxygen is reduced or completely removed from the formulations peptidomimetic macrocycles. The time period for sparging depends in several factors including the amount of formulation, the effectiveness of agitation and the flow rate of the inert gas. In some embodiments, sparging is done by bubbling the inert gas through the formulations for a period of about 1 min-12 h. In some embodiments the formulations are sparged for a period of about 1 min-about 11 h, about 1 min-about 10 h, about 1 min-9 h, about 1 min-8 h, about 1 min-7 h, about 1 min-6 h, about 1 min-5 h, about 1 min-4 h, about 1 min-3 h, about 1 min-2 h, about 1 min-1 h, about 1 min-45 min, about 1 min-about 30 min, about 1 min-15 min, about 1 min-10 min, about 1 min-about 9 min, about 1 min-8 min, about 1 min-about 7 min, about 1 min-6 min, about 1 min-about 5 min, about 1 min-about 4 min, about 1 min-about 3 min, about 1 min-about 2 min. In some embodiments, sparging is performed for less than about 1 minute.

Methods of Use

Methods

In one aspect, provided herein are aqueous pharmaceutical formulations that are useful in competitive binding assays to identify agents which bind to the natural ligand(s) of the proteins or peptides upon which the peptidomimetic macrocycles are modeled. For example, in the p53/MDMX system, labeled peptidomimetic macrocycles based on p53 can be used in a MDMX binding assay along with small molecules that competitively bind to MDMX. Competitive binding studies allow for rapid in vitro evaluation and determination of drug candidates specific for the p53/MDMX system. Such binding studies can be performed with any of the peptidomimetic macrocycles disclosed herein and their binding partners.
Further provided are methods for the generation of antibodies against the peptidomimetic macrocycles. In some embodiments, these antibodies specifically bind both the peptidomimetic macrocycle and the precursor peptides, such as p53, to which the peptidomimetic macrocycles are related. Such antibodies, for example, disrupt the native protein-protein interaction, for example, binding between p53 and MDMX.
In other aspects, provided herein are both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with aberrant (e.g., insufficient or excessive) expression or activity of the molecules including p53, MDM2 or MDMX.
In another embodiment, a disorder is caused, at least in part, by an abnormal level of p53 or MDM2 or MDMX, (e.g., over or under expression), or by the presence of p53 or MDM2 or MDMX exhibiting abnormal activity. As such, the reduction in the level and/or activity of p53 or MDM2 or MDMX, or the enhancement of the level and/or activity of p53 or MDM2 or MDMX, by peptidomimetic macrocycles derived from p53, is used, for example, to ameliorate or reduce the adverse symptoms of the disorder.
In another aspect, provided herein are methods for treating or preventing a disease including hyperproliferative disease and inflammatory disorder by interfering with the interaction or binding between binding partners, for example, between p53 and MDM2 or p53 and MDMX. These methods comprise administering an effective amount of a compound to a warm blooded animal, including a human. In some embodiments, the administration of one or more compounds disclosed herein induces cell growth arrest or apoptosis.
As used herein, the term “treatment” is defined as the application or administration of a therapeutic agent to a patient, or application or administration of a therapeutic agent to an isolated tissue or cell line from a patient, who has a disease, a symptom of disease or a predisposition toward a disease, with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, improve or affect the disease, the symptoms of disease or the predisposition toward disease.

Disease and Disorders

In some embodiments, the pharmaceutical formulations can be used to treat, prevent, and/or diagnose cancers and neoplastic conditions. As used herein, the terms “cancer”, “hyperproliferative” and “neoplastic” refer to cells having the capacity for autonomous growth, i.e., an abnormal state or condition characterized by rapidly proliferating cell growth. Hyperproliferative and neoplastic disease states can be categorized as pathologic, i.e., characterizing or constituting a disease state, or can be categorized as non-pathologic, i.e., a deviation from normal but not associated with a disease state. The term is meant to include all types of cancerous growths or oncogenic processes, metastatic tissues or malignantly transformed cells, tissues, or organs, irrespective of histopathology type or stage of invasiveness. A metastatic tumor can arise from a multitude of primary tumor types, including but not limited to those of breast, lung, liver, colon and ovarian origin. “Pathologic hyperproliferative” cells occur in disease states characterized by malignant tumor growth. Examples of non-pathologic hyperproliferative cells include proliferation of cells associated with wound repair. Examples of cellular proliferative and/or differentiative disorders include cancer, e.g., carcinoma, sarcoma, or metastatic disorders. In some embodiments, the pharmaceutical formulations can be used for controlling/treating breast cancer, ovarian cancer, colon cancer, lung cancer, metastasis of such cancers and the like.
Examples of cancers or neoplastic conditions include, but are not limited to, a fibrosarcoma, myosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangiosarcoma, lymphangioendotheliosarcoma, synovioma, mesothelioma, Ewing's tumor, leiomyosarcoma, rhabdomyosarcoma, gastric cancer, esophageal cancer, rectal cancer, pancreatic cancer, ovarian cancer, prostate cancer, uterine cancer, cancer of the head and neck, skin cancer, brain cancer, squamous cell carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinoma, cystadenocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilm's tumor, cervical cancer, testicular cancer, small cell lung carcinoma, non-small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroma, oligodendroglioma, meningioma, melanoma, neuroblastoma, retinoblastoma, leukemia, lymphoma, Kaposi sarcoma, or glioblastoma multiforme.
In some embodiments, the cancer is head and neck cancer, melanoma, lung cancer, breast cancer, or glioma.
In some examples, the cancer is pancreatic cancer, bladder cancer, colon cancer, liver cancer, colorectal cancer (colon cancer or rectal cancer), breast cancer, prostate cancer, renal cancer, hepatocellular cancer, lung cancer, ovarian cancer, cervical cancer, gastric cancer, esophageal cancer, head and neck cancer, melanoma, neuroendocrine cancers, CNS cancers, brain tumors, bone cancer, skin cancer, ocular tumor, choriocarcinoma (tumor of the placenta), sarcoma or soft tissue cancer.
In some examples, cancer is bladder cancer, bone cancer, breast cancer, cervical cancer, CNS cancer, colon cancer, ocular tumor, renal cancer, liver cancer, lung cancer, pancreatic cancer, choriocarcinoma (tumor of the placenta), prostate cancer, sarcoma, skin cancer, soft tissue cancer or gastric cancer.
In some examples, the cancer is breast cancer. Non limiting examples of breast cancer that can be treated by the instant pharmaceutical formulations include ductal carcinoma in situ (DCIS or intraductal carcinoma), lobular carcinoma in situ (LCIS), invasive (or infiltrating) ductal carcinoma, invasive (or infiltrating) lobular carcinoma, inflammatory breast cancer, triple-negative breast cancer, paget disease of the nipple, phyllodes tumor (phylloides tumor or cystosarcoma phyllodes), angiosarcoma, adenoid cystic (or adenocystic) carcinoma, low-grade adenosquamous carcinoma, medullary carcinoma, papillary carcinoma, tubular carcinoma, metaplastic carcinoma, micropapillary carcinoma, and mixed carcinoma.
In some examples, the cancer is bone cancer. Non limiting examples of bone cancer that can be treated by the instant pharmaceutical formulations include osteosarcoma, chondrosarcoma, the Ewing Sarcoma Family of Tumors (ESFTs).
In some examples, the cancer is skin cancer. Non limiting examples of skin cancer that can be treated by the instant pharmaceutical formulations include melanoma, basal cell skin cancer, and squamous cell skin cancer.
In some examples, the cancer is ocular tumor. Non limiting examples of ocular tumor that can be treated by the pharmaceutical formulations of the instant disclosure include ocular tumor is choroidal nevus, choroidal melanoma, choroidal metastasis, choroidal hemangioma, choroidal osteoma, iris melanoma, uveal melanoma, melanocytoma, metastasis retinal capillary hemangiomas, congenital hypertrophy of the RPE, RPE adenoma or retinoblastoma.
Examples of proliferative disorders include hematopoietic neoplastic disorders. As used herein, the term “hematopoietic neoplastic disorders” includes diseases involving hyperplastic/neoplastic cells of hematopoietic origin, e.g., arising from myeloid, lymphoid or erythroid lineages, or precursor cells thereof. The diseases can arise from poorly differentiated acute leukemias, e.g., erythroblastic leukemia and acute megakaryoblastic leukemia. Additional exemplary myeloid disorders include, but are not limited to, acute promyeloid leukemia (APML), acute myelogenous leukemia (AML) and chronic myelogenous leukemia (CML) (reviewed in Vaickus (1991), Crit Rev. Oncol./Hemotol. 11:267-97); lymphoid malignancies include, but are not limited to acute lymphoblastic leukemia (ALL) which includes B-lineage ALL and T-lineage ALL, chronic lymphocytic leukemia (CLL), prolymphocytic leukemia (PLL), hairy cell leukemia (HLL) and Waldenstrom's macroglobulinemia (WM). Additional forms of malignant lymphomas include, but are not limited to non-Hodgkin lymphoma and variants thereof, peripheral T cell lymphomas, adult T cell leukemia/lymphoma (ATL), cutaneous T-cell lymphoma (CTCL), large granular lymphocytic leukemia (LGF), Hodgkin's disease and Reed-Stemberg disease.
Examples of cellular proliferative and/or differentiative disorders of the breast include, but are not limited to, proliferative breast disease including, e.g., epithelial hyperplasia, sclerosing adenosis, and small duct papillomas; tumors, e.g., stromal tumors such as fibroadenoma, phyllodes tumor, and sarcomas, and epithelial tumors such as large duct papilloma; carcinoma of the breast including in situ (noninvasive) carcinoma that includes ductal carcinoma in situ (including Paget's disease) and lobular carcinoma in situ, and invasive (infiltrating) carcinoma including, but not limited to, invasive ductal carcinoma, invasive lobular carcinoma, medullary carcinoma, colloid (mucinous) carcinoma, tubular carcinoma, and invasive papillary carcinoma, and miscellaneous malignant neoplasms. Disorders in the male breast include, but are not limited to, gynecomastia and carcinoma.
Examples of cellular proliferative and/or differentiative disorders of the skin include, but are not limited to proliferative skin disease such as melanomas, including mucosal melanoma, superficial spreading melanoma, nodular melanoma, lentigo (e.g. lentigo maligna, lentigo maligna melanoma, or acral lentiginous melanoma), amelanotic melanoma, desmoplastic melanoma, melanoma with features of a Spitz nevus, melanoma with small nevus-like cells, polypoid melanoma, and soft-tissue melanoma; basal cell carcinomas including micronodular basal cell carcinoma, superficial basal cell carcinoma, nodular basal cell carcinoma (rodent ulcer), cystic basal cell carcinoma, cicatricial basal cell carcinoma, pigmented basal cell carcinoma, aberrant basal cell carcinoma, infiltrative basal cell carcinoma, nevoid basal cell carcinoma syndrome, polypoid basal cell carcinoma, pore-like basal cell carcinoma, and fibroepithelioma of Pinkus; squamus cell carcinomas including acanthoma (large cell acanthoma), adenoid squamous cell carcinoma, basaloid squamous cell carcinoma, clear cell squamous cell carcinoma, signet-ring cell squamous cell carcinoma, spindle cell squamous cell carcinoma, Marjolin's ulcer, erythroplasia of Queyrat, and Bowen's disease; or other skin or subcutaneous tumors.
Examples of cellular proliferative and/or differentiative disorders of the lung include, but are not limited to, bronchogenic carcinoma, including paraneoplastic syndromes, bronchioloalveolar carcinoma, neuroendocrine tumors, such as bronchial carcinoid, miscellaneous tumors, and metastatic tumors; pathologies of the pleura, including inflammatory pleural effusions, noninflammatory pleural effusions, pneumothorax, and pleural tumors, including solitary fibrous tumors (pleural fibroma) and malignant mesothelioma.
Examples of cellular proliferative and/or differentiative disorders of the colon include, but are not limited to, non-neoplastic polyps, adenomas, familial syndromes, colorectal carcinogenesis, colorectal carcinoma, and carcinoid tumors.
Examples of cellular proliferative and/or differentiative disorders of the liver include, but are not limited to, nodular hyperplasias, adenomas, and malignant tumors, including primary carcinoma of the liver and metastatic tumors. Examples of cellular proliferative and/or differentiative disorders of the ovary include, but are not limited to, ovarian tumors such as, tumors of coelomic epithelium, serous tumors, mucinous tumors, endometrioid tumors, clear cell adenocarcinoma, cystadenofibroma, Brenner tumor, surface epithelial tumors; germ cell tumors such as mature (benign) teratomas, monodermal teratomas, immature malignant teratomas, dysgerminoma, endodermal sinus tumor, choriocarcinoma; sex cord-stomal tumors such as, granulosa-theca cell tumors, thecomafibromas, androblastomas, hill cell tumors, and gonadoblastoma; and metastatic tumors such as Krukenberg tumors.
In one aspect, the present invention provides novel peptidomimetic macrocycles that are useful in competitive binding assays to identify agents which bind to the natural ligand(s) of the proteins or peptides upon which the peptidomimetic macrocycles are modeled. For example, in the BH3/BCL-X_Lanti-apoptotic system labeled peptidomimetic macrocycles based on BH3 can be used in a BCL-X_Lbinding assay along with small molecules that competitively bind to BCL-X_L. Competitive binding studies allow for rapid in vitro evaluation and determination of drug candidates specific for the BH3/BCL-X_Lsystem. The invention further provides for the generation of antibodies against the peptidomimetic macrocycles. In some embodiments, these antibodies specifically bind both the peptidomimetic macrocycle and the BH3 peptidomimetic precursors upon which the peptidomimetic macrocycles are derived. Such antibodies, for example, disrupt the BH3/BCL-XL systems, respectively.
In other aspects, the present invention provides for both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with aberrant (e.g., insufficient or excessive) BCL-2 family member expression or activity (e.g., extrinsic or intrinsic apoptotic pathway abnormalities). It is believed that some BCL-2 type disorders are caused, at least in part, by an abnormal level of one or more BCL-2 family members (e.g., over or under expression), or by the presence of one or more BCL-2 family members exhibiting abnormal activity. As such, the reduction in the level and/or activity of the BCL-2 family member or the enhancement of the level and/or activity of the BCL-2 family member, is used, for example, to ameliorate or reduce the adverse symptoms of the disorder.
In one embodiment, the compounds of the invention are used to treat disorders associated with expression or overexpression of Mcl-1. Mcl-1 has been shown to be expressed in many tissues and neoplastic cell lines and is thought to participate in the development of malignancies (Thallinger et al. (2004) Clin. Cancer Res. 10:4185-4191). The peptidomimetic macrocycles of the invention can be used for the treatment of such malignancies.
In one embodiment, the disorder being treated (e.g. cancer) is differentially responsive to the peptidomimetic macrocycles of the invention. In some embodiments, the cancer is treated with a BIM peptidomimetic macrocycle and is at least 2-fold less sensitive to treatment using a BID polypeptide (such as a BID peptidomimetic macrocycle or uncrosslinked polypeptide) as measured in an in vitro cell viability assay. In other embodiments, the cancer is at least 5-fold less sensitive to treatment using a BID polypeptide as measured in an in vitro cell viability assay. In yet other embodiments, the cancer is at least 8-fold less sensitive to treatment using a BID polypeptide as measured in an in vitro cell viability assay. In other embodiments, the cancer is treated with a BID peptidomimetic macrocycle and is at least 2-fold less sensitive to treatment using a BIM polypeptide (such as a BIM peptidomimetic macrocycle or uncrosslinked polypeptide) as measured in an in vitro cell viability assay. In other embodiments, the cancer is at least 5-fold less sensitive to treatment using a BIM polypeptide as measured in an in vitro cell viability assay. In yet other embodiments, the cancer is at least 8-fold less sensitive to treatment using a BIM polypeptide as measured in an in vitro cell viability assay.
In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of a BCL-family protein and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of the BCL-family protein is detected. BCL-family proteins include, for example, BCL-2, BCL-X_L, MCL-1, Bfl1/A1, BOO/DIVA, NRH/NR13, BAX, BAD, BAK, BOK, BIK, PUMA, BIM, BMF, BLK, BNIP3, HRK, NIX, SPIKE, and Noxa. In one embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BCL-2 in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BCL-2 is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BCL-X_Lin the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BCL-X_Lis detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of MCL-1 in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of MCL-1 is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BAX in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BAX is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BAD in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BAD is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BAK in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BAK is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of PUMA in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of PUMA is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of Noxa in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of Noxa is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of Noxa in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of Noxa is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of Bfl1/A1 in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of Bfl1/A1 is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BOO/DIVA in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BOO/DIVA is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of NRH/NR13 in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of NRH/NR13 is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BOK in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BOK is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BIK in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BIK is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BMF in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BMF is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BLK in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BLK is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of BNIP3 in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of BNIP3 is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of HRK in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of HRK is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of Nix in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of Nix is detected. In another embodiment, a method of treating a human patient is provided comprising performing an assay to evaluate the levels of SPIKE in the patient and administering to the patient a peptidomimetic macrocycle if an aberrant or irregular level of expression of SPIKE is detected.
In one aspect, the invention provides methods of treating breast cancer by administering the peptidomimetic macrocycles of the invention. Breast cancer includes invasive breast carcinomas, such as invasive ductal carcinoma, invasive lobular carcinoma, tubular carcinoma, invasive cribriform carcinoma, medullary carcinoma, mucinous carcinoma and other tumours with abundant mucin, cystadenocarcinoma, columnar cell mucinous carcinoma, signet ring cell carcinoma, neuroendocrine tumours (including solid neuroendocrine carcinoma, atypical carcinoid tumour, small cell/oat cell carcinoma, or large cell neuroendocrine carcioma), invasive papillary carcinoma, invasive micropapillary carcinoma, apocrine carcinoma, metaplastic carcinomas, pure epithelial metaplastic carciomas, mixed epithelial/mesenchymal metaplastic carcinomas, lipid-rich carcinoma, secretory carcinoma, oncocytic carcinoma, adenoid cystic carcinoma, acinic cell carcinoma, glycogen-rich clear cell carcinoma, sebaceous carcinoma, inflammatory carcinoma or bilateral breast carcinoma; mesenchymal tumors such as haemangioma, angiomatosis, haemangiopericytoma, pseudoangiomatous stromal hyperplasia, myofibroblastoma, fibromatosis (aggressive), inflammatory myofibroblastic tumour, lipoma, angiolipoma, granular cell tumour, neurofibroma, schwannoma, angiosarcoma, liposarcoma, rhabdomyosarcoma, osteosarcoma, leiomyoma, or leiomysarcoma; myoepithelial lesions such as myoepitheliosis, adenomyoepithelial adenosis, adenomyoepithelioma, or malignant myoepithelioma; fibroepithelial tumours such as fibroadenoma, phyllodes tumour, low grade periductal stromal sarcoma, or mammary hamartoma; and tumours of the nipple such as nipple adenoma, syringomatous adenoma, or Paget's disease of the nipple.
Treatment of breast cancer can be effected in conjunction with any additional therapy, such as a therapy that is part of the standard of care. A surgical technique such as lumpectomy or mastectomy can be performed prior to, during, or following treatment with the peptidomimetic macrocycles of the invention. Alternatively, radiation therapy can be used for the treatment of breast cancer in conjunction with the peptidomimetic macrocycles of the invention. In other cases, the peptidomimetic macrocycles of the invention are administered in combination with a second therapeutic agent. Such an agent can be a chemotherapeutic agent such as an individual drug or combination of drugs and therapies. For example, the chemotherapeutic agent can be an adjuvant chemotherapeutic treatment such as CMF (cyclophosphamide, methotrexate, and 5-fluorouracil); FAC or CAF (5-fluorouracil, doxorubicin, cyclophosphamide); AC or CA (doxorubicin and cyclophosphamide); AC-Taxol (AC followed by paclitaxel); TAC (docetaxel, doxorubicin, and cyclophosphamide); FEC (5-fluorouracil, epirubicin and cyclophosphamide); FECD (FEC followed by docetaxel); TC (docetaxel and cyclophosphamide). In addition to chemotherapy, trastuzumab can also be added to the regimen depending on the tumor characteristics (i.e. HER2/neu status) and risk of relapse. Hormonal therapy can also be appropriate before, during or following chemotherapeutic treatment. For example, tamoxifen can be administered or a compound in the category of aromatase inhibitors including, but not limited to aminogluthetimide, anastrozole, exemestane, formestane, letrozole, or vorozole. In other embodiments, an antiangiogenic agent can be used in combination therapy for the treatment of breast cancer. The antiangiogenic agent can be an anti-VEGF agent including, but not limited to bevacizumab.
In another aspect, the peptidomimetic macrocycles of the invention can be used to treat ovarian cancer. Ovarian cancers include ovarian tumors such as, tumors of coelomic epithelium, serous tumors, mucinous tumors, endometrioid tumors, clear cell adenocarcinoma, cystadenofibroma, Brenner tumor, surface epithelial tumors; germ cell tumors such as mature (benign) teratomas, monodermal teratomas, immature malignant teratomas, dysgerminoma, endodermal sinus tumor, choriocarcinoma; sex cord-stomal tumors such as, granulosa-theca cell tumors, thecomafibromas, androblastomas, hill cell tumors, and gonadoblastoma; and metastatic tumors such as Krukenberg tumors.
The peptidomimetic macrocycles of the invention can be administered in conjunction with a second therapy such as a therapy that is part of the standard of care. Surgery, immunotherapy, chemotherapy, hormone therapy, radiation therapy, or a combination thereof, are some possible treatments available for ovarian cancer. Some possible surgical procedures include debulking, and a unilateral or bilateral oophorectomy and/or a unilateral or bilateral salpigectomy.
Anti-cancer drugs that can be used include cyclophosphamide, etoposide, altretamine, and ifosfamide. Hormone therapy with the drug tamoxifen can be used to shrink ovarian tumors. Radiation therapy can be external beam radiation therapy and/or brachytherapy.
In another aspect, the peptidomimetic macrocycles of the invention can be used to treat prostate cancer. Prostate cancers include adenocarcinomas and metastasized adenocarcinomas. The peptidomimetic macrocycles of the invention can be administered in conjunction with a second therapy such as a therapy that is part of the standard of care. Treatment for prostate cancer can involve surgery, radiation therapy, High Intensity Focused Ultrasound (HIFU), chemotherapy, cryosurgery, hormonal therapy, or any combination thereof. Surgery can involve prostatectomy, radical perineal prostatectomy, laparoscopic radical prostatectomy, transurethral resection of the prostate or orchiectomy. Radiation therapy can include external beam radiation therapy and/or brachytherapy. Hormonal therapy can include orchiectomy; administration of antiandrogens such as flutamide, bicalutamide, nilutamide, or cyproterone acetate; medications which inhibit the production of adrenal androgens such as DHEA, such as ketoconazole and aminoglutethimide; and GnRH antagonists or agonists such as Abarelix (Plenaxis®), Cetrorelix (Cetrotide®), Ganirelix (Antagon®), leuprolide, goserelin, triptorelin, or buserelin. Treatment with an anti-androgen agent, which blocks androgen activity in the body, is another available therapy. Such agents include flutamide, bicalutamide, and nilutamide. This therapy is typically combined with LHRH analog administration or an orchiectomy, which is termed a combined androgen blockade (CAB). Chemotherapy includes, but is not limited to, administration of docetaxel, for example with a corticosteroid such as prednisone. Anti-cancer drugs such as doxorubicin, estramustine, etoposide, mitoxantrone, vinblastine, paclitaxel, carboplatin can also be administered to slow the growth of prostate cancer, reduce symptoms and improve the quality of life. Additional compounds such as bisphosphonate drugs can also be administered.
In another aspect, the peptidomimetic macrocycles of the invention can be used to treat renal cancer. Renal cancers include, but are not limited to, renal cell carcinomas, metastases from extra-renal primary neoplasms, renal lymphomas, squamous cell carcinomas, juxtaglomerular tumors (reninomas), transitional cell carcinomas, angiomyolipomas, oncocytomas and Wilm's tumors. The peptidomimetic macrocycles of the invention can be administered in conjunction with a second therapy such as a therapy that is part of the standard of care. Treatment for renal cancer can involve surgery, percutaneous therapies, radiation therapies, chemotherapy, vaccines, or other medication. Surgical techniques useful for treatment of renal cancer in combination with the peptidomimetic macrocycles of the invention include nephrectomy, which can include removal of the adrenal gland, retroperitoneal lymph nodes, and any other surrounding tissues affected by the invasion of the tumor. Percutaneous therapies include, for example, image-guided therapies which can involve imaging of a tumor followed by its targeted destruction by radiofrequency ablation or cryotherapy. In some cases, other chemotherapeutic or other medications useful in treating renal cancer can be α-interferon, interleukin-2, bevacizumab, sorafenib, sunitib, temsirolimus or other kinase inhibitors.
In other aspects, the invention provides methods of treating pancreatic cancer by administering peptidomimetic macrocycles of the invention, such as a pancreatic cancer selected from the following: an epitheliod carcinoma in the pancreatic duct tissue and an adenocarcinoma in a pancreatic duct. The most common type of pancreatic cancer is an adenocarcinoma, which occurs in the lining of the pancreatic duct. Possible treatments available for pancreatic cancer include surgery, immunotherapy, radiation therapy, and chemotherapy. Possible surgical treatment options include a distal or total pancreatectomy and a pancreaticoduodenectomy (Whipple procedure). Radiation therapy can be an option for pancreatic cancer patients, specifically external beam radiation where radiation is focused on the tumor by a machine outside the body. Another option is intraoperative electron beam radiation administered during an operation. Chemotherapy can also be used to treat pancreatic cancer patients. Suitable anti-cancer drugs include, but are not limited to, 5-fluorouracil (5-FU), mitomycin, ifosfamide, doxorubicin, streptozocin, chlorozotocin, and combinations thereof. The methods provided by the invention can provide a beneficial effect for pancreatic cancer patients, by administration of a polypeptide of the invention or a combination of administration of a peptidomimetic macrocycle and surgery, radiation therapy, or chemotherapy.
In one aspect, peptidomimetic macrocycles of the invention can be used for the treatment of colon cancer, including but not limited to non-neoplastic polyps, adenomas, familial syndromes, colorectal carcinogenesis, colorectal carcinoma, and carcinoid tumors. Possible treatments available for colon cancer that can be used in conjunction with the peptidomimetic macrocycles of the invention include surgery, chemotherapy, radiation therapy or targeted drug therapy.
Radiation therapy can include external beam radiation therapy and/or brachytherapy. Chemotherapy can be used to reduce the likelihood of metastasis developing, shrink tumor size, or slow tumor growth. Chemotherapy is often applied after surgery (adjuvant), before surgery (neo-adjuvant), or as the primary therapy if surgery is not indicated (palliative). For example, exemplary regimens for adjuvant chemotherapy involve the combination of infusional 5-fluorouracil, leucovorin, and oxaliplatin (FOLFOX). First line chemotherapy regimens can involve the combination of infusional 5-fluorouracil, leucovorin, and oxaliplatin (FOLFOX) with a targeted drug such as bevacizumab, cetuximab or panitumumab or infusional 5-fluorouracil, leucovorin, and irinotecan (FOLFIRI) with targeted drug such as bevacizumab, cetuximab or panitumumab. Other chemotherapeutic agents that can be useful in the treatment or prevention of colon cancer in combination with the peptidomimetic macrocycles of the invention are Bortezomib (Velcade®), Oblimersen (Genasense®, G3139), Gefitinib and Erlotinib (Tarceva®) and Topotecan (Hycamtin®).
Some embodiments provide methods for the treatment of lung cancer using the peptidomimetic macrocycles of the invention. Examples of cellular proliferative and/or differentiative disorders of the lung include, but are not limited to, bronchogenic carcinoma, including paraneoplastic syndromes, bronchioloalveolar carcinoma, neuroendocrine tumors, such as bronchial carcinoid, miscellaneous tumors, and metastatic tumors; pathologies of the pleura, including inflammatory pleural effusions, noninflammatory pleural effusions, pneumothorax, and pleural tumors, including solitary fibrous tumors (pleural fibroma) and malignant mesothelioma.
The most common type of lung cancer is non-small cell lung cancer (NSCLC), which accounts for approximately 80-85% of lung cancers and is divided into squamous cell carcinomas, adenocarcinomas, and large cell undifferentiated carcinomas. Small cell lung cancer, e.g. small cell lung carcinomas, accounts for 15-20% of lung cancers. Treatment options for lung cancer include surgery, immunotherapy, radiation therapy, chemotherapy, photodynamic therapy, or a combination thereof. Some possible surgical options for treatment of lung cancer are a segmental or wedge resection, a lobectomy, or a pneumonectomy. Radiation therapy can be external beam radiation therapy or brachytherapy. Some anti-cancer drugs that can be used in chemotherapy to treat lung cancer in combination with the peptidomimetic macrocycles of the invention include cisplatin, carboplatin, paclitaxel, docetaxel, gemcitabine, vinorelbine, irinotecan, etoposide, vinblastine, gefitinib, ifosfamide, methotrexate, or a combination thereof. Photodynamic therapy (PDT) can be used to treat lung cancer patients. The methods described herein can provide a beneficial effect for lung cancer patients, by administration of a peptidomimetic macrocycle or a combination of administration of a peptidomimetic macrocycle and surgery, radiation therapy, chemotherapy, photodynamic therapy, or a combination thereof.
Examples of cellular proliferative and/or differentiative disorders of the liver include, but are not limited to, nodular hyperplasias, adenomas, and malignant tumors, including primary carcinoma of the liver and metastatic tumors.
Immunoproliferative disorders (also known as “immunoproliferative diseases” or “immunoproliferative neoplasms”) are disorders of the immune system that are characterized by the abnormal proliferation of the primary cells of the immune system, which includes B cells, T cells and Natural Killer (NK) cells, or by the excessive production of immunoglobulins (also known as antibodies). Such disorders include the general categories of lymphoproliferative disorders, hypergammaglobulinemias, and paraproteinemias. Examples of such disorders include, but are not limited to, X-linked lymphoproliferative disorder, autosomal lymphoproliferative disorder, Hyper-IgM syndrome, heavy chain disease, and cryoglobulinemia. Other immunoproliferative disorders can be graft versus host disease (GVHD); psoriasis; immune disorders associated with graft transplantation rejection; T cell lymphoma; T cell acute lymphoblastic leukemia; testicular angiocentric T cell lymphoma; benign lymphocytic angiitis; and autoimmune diseases such as lupus erythematosus, Hashimoto's thyroiditis, primary myxedema, Graves' disease, pernicious anemia, autoimmune atrophic gastritis, Addison's disease, insulin dependent diabetes mellitis, good pasture's syndrome, myasthenia gravis, pemphigus, Crohn's disease, sympathetic ophthalmia, autoimmune uveitis, multiple sclerosis, autoimmune hemolytic anemia, idiopathic thrombocytopenia, primary biliary cirrhosis, chronic action hepatitis, ulceratis colitis, Sjogren's syndrome, rheumatoid arthritis, polymyositis, scleroderma, and mixed connective tissue disease.
Combination Treatments
In one embodiment, peptidomimetic macrocycles of the invention can be used for the treatment of cancer in conjunction with alkylating and alkylating-like agents. Such agents include, for example, nitrogen mustards such as chlorambucil, chlormethine, cyclophosphamide, ifosfamide, and melphalan; nitrosoureas such as carmustine, fotemustine, lomustine, and streptozocin; platinum therapeutic agents such as carboplatin, cisplatin, oxaliplatin, BBR3464, and satraplatin; or other agents, including but not limited to busulfan, dacarbazine, procarbazine, temozolomide, thiotepa, treosulfan, or uramustine.
In another embodiment, peptidomimetic macrocycles of the invention can be used in conjunction with an antineoplastic agent which is an antimetabolite. For example, such an antineoplastic agent can be a folic acid such as aminopterin, methotrexate, pemetrexed, or raltitrexed. Alternatively, the antineoplastic agent can be a purine, including but not limited to cladribine, clofarabine, fludarabine, mercaptopurine, pentostatin, thioguanine. In further embodiments, the antineoplastic agent can be a pyrimidine such as capecitabine, cytarabine, fluorouracil, floxuridine, and gemcitabine.
In still other embodiments, peptidomimetic macrocycles of the invention can be used in conjunction with an antineoplastic agent which is an spindle poison/mitotic inhibitor. Agents in this category include taxanes, for example docetaxel and paclitaxel; and vinca alkaloids such as vinblastine, vincristine, vindesine, and vinorelbine. In yet other embodiments, peptidomimetic macrocycles of the invention can be used in combination with an antineoplastic agent which is a cytotoxic/antitumor antibiotic from the anthracycline family such as daunorubicin, doxorubicin, epirubicin, idarubicin, mitoxantrone, pixantrone, or valrubicin; an antibiotic from the Streptomyces family such as actinomycin, bleomycin, mitomycin, or plicamycin; or hydroxyurea. Alternatively, agents used for combination therapy can be topoisomerase inhibitors including, but not limited to camptothecin, topotecan, irinotecan, etoposide, or teniposide.
Alternatively, the antineoplastic agent can be an antibody or antibody-derived agent. For example, a receptor tyrosine kinase-targeted antibody such as cetuximab, panitumumab, or trastuzumab can be used. Alternatively, the antibody can be an anti-CD20 antibody such as rituximab or tositumomab, or any other suitable antibody including but not limited to alemtuzumab, bevacizumab, and gemtuzumab. In other embodiments, the antineoplastic agent is a photosensitizer such as aminolevulinic acid, methyl aminolevulinate, porfimer sodium, or verteporfin. In still other embodiments, the antineoplastic agent is a tyrosine kinase inhibitor such as dediranib, dasatinib, erlotinib, gefitinib, imatinib, lapatinib, nilotinib, sorafenib, sunitinib, or vandetanib. Other neoplastic agents suitable in the use of the invention include, for example, alitretinoin, tretinoin, altretamine, amsacrine, anagrelide, arsenic trioxide, asparaginase (pegaspargase), bexarotene, bortezomib, denileukin diftitox, estramustine, ixabepilone, masoprocol, or mitotane.
In other or further embodiments, the peptidomimetics macrocycles described herein are used to treat, prevent or diagnose conditions characterized by overactive cell death or cellular death due to physiologic insult, etc. Some examples of conditions characterized by premature or unwanted cell death are or alternatively unwanted or excessive cellular proliferation include, but are not limited to hypocellular/hypoplastic, acellular/aplastic, or hypercellular/hyperplastic conditions. Some examples include hematologic disorders including but not limited to fanconi anemia, aplastic anemia, thalaessemia, congenital neutropenia, myelodysplasia
In other or further embodiments, the peptidomimetics macrocycles of the invention that act to decrease apoptosis are used to treat disorders associated with an undesirable level of cell death. Thus, in some embodiments, the anti-apoptotic peptidomimetics macrocycles of the invention are used to treat disorders such as those that lead to cell death associated with viral infection, e.g., infection associated with infection with human immunodeficiency virus (HIV). A wide variety of neurological diseases are characterized by the gradual loss of specific sets of neurons, and the anti-apoptotic peptidomimetics macrocycles of the invention are used, in some embodiments, in the treatment of these disorders. Such disorders include Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis (ALS) retinitis pigmentosa, spinal muscular atrophy, and various forms of cerebellar degeneration. The cell loss in these diseases does not induce an inflammatory response, and apoptosis appears to be the mechanism of cell death. In addition, a number of hematologic diseases are associated with a decreased production of blood cells. These disorders include anemia associated with chronic disease, aplastic anemia, chronic neutropenia, and the myelodysplastic syndromes. Disorders of blood cell production, such as myelodysplastic syndrome and some forms of aplastic anemia, are associated with increased apoptotic cell death within the bone marrow. These disorders could result from the activation of genes that promote apoptosis, acquired deficiencies in stromal cells or hematopoietic survival factors, or the direct effects of toxins and mediators of immune responses. Two common disorders associated with cell death are myocardial infarctions and stroke. In both disorders, cells within the central area of ischemia, which is produced in the event of acute loss of blood flow, appear to die rapidly as a result of necrosis. However, outside the central ischemic zone, cells die over a more protracted time period and morphologically appear to die by apoptosis.
In other or further embodiments, the anti-apoptotic peptidomimetics macrocycles of the invention are used to treat all such disorders associated with undesirable cell death.
Some examples of immunologic disorders that are treated with the peptidomimetics macrocycles described herein include but are not limited to organ transplant rejection, arthritis, lupus, IBD, Crohn's disease, asthma, multiple sclerosis, diabetes, etc.
Some examples of neurologic disorders that are treated with the peptidomimetics macrocycles described herein include but are not limited to Alzheimer's Disease, Down's Syndrome, Dutch Type Hereditary Cerebral Hemorrhage Amyloidosis, Reactive Amyloidosis, Familial Amyloid Nephropathy with Urticaria and Deafness, Muckle-Wells Syndrome, Idiopathic Myeloma; Macroglobulinemia-Associated Myeloma, Familial Amyloid Polyneuropathy, Familial Amyloid Cardiomyopathy, Isolated Cardiac Amyloid, Systemic Senile Amyloidosis, Adult Onset Diabetes, Insulinoma, Isolated Atrial Amyloid, Medullary Carcinoma of the Thyroid, Familial Amyloidosis, Hereditary Cerebral Hemorrhage With Amyloidosis, Familial Amyloidotic Polyneuropathy, Scrapie, Creutzfeldt-Jacob Disease, Gerstmann Straussler-Scheinker Syndrome, Bovine Spongiform Encephalitis, a prion-mediated disease, and Huntington's Disease.
Some examples of endocrinologic disorders that are treated with the peptidomimetics macrocycles described herein include but are not limited to diabetes, hypothyroidism, hypopituitarism, hypoparathyroidism, hypogonadism, etc.
Examples of cardiovascular disorders (e.g., inflammatory disorders) that are treated or prevented with the peptidomimetics macrocycles of the invention include, but are not limited to, atherosclerosis, myocardial infarction, stroke, thrombosis, aneurism, heart failure, ischemic heart disease, angina pectoris, sudden cardiac death, hypertensive heart disease; non-coronary vessel disease, such as arteriolosclerosis, small vessel disease, nephropathy, hypertriglyceridemia, hypercholesterolemia, hyperlipidemia, xanthomatosis, asthma, hypertension, emphysema and chronic pulmonary disease; or a cardiovascular condition associated with interventional procedures (“procedural vascular trauma”), such as restenosis following angioplasty, placement of a shunt, stent, synthetic or natural excision grafts, indwelling catheter, valve or other implantable devices. Preferred cardiovascular disorders include atherosclerosis, myocardial infarction, aneurism, and stroke.
In some embodiments, the peptidomimetic macrocycles are useful in the treatment of viral disorders. For example, in the PB1/PA system, labeled peptidomimetic macrocycles based on PB1 can be used in a PA binding assay along with small molecules that competitively bind to PA. Competitive binding studies allow for rapid in vitro evaluation and determination of drug candidates specific for the PB1/PA system. Such binding studies can be performed with any of the peptidomimetic macrocycles disclosed herein and their binding partners. Antibodies can also be developed which disrupt the binding between PA and PD1.
For example, peptidomimetic macrocycles derived from the PB1 helix sequence, or peptidomimetic macrocycles that bind selectively to the PB1 peptide binding site of the PA protein, can selectively inhibit influenza RNA-dependent RNA polymerases. Peptidomimetic macrocycles derived from the PB2 helix sequence, or peptidomimetic macrocycles that bind selectively to the PB2 peptide binding site of the PB1 protein, can selectively inhibit influenza RNA-dependent RNA polymerases. When administered within a therapeutic window after infection, such peptidomimetic macrocycles can reduce the severity or duration of an influenza infection. When administered prophylactically, such peptidomimetic macrocycles can prevent infection by influenza viruses and thereby decrease the spread of influenza and reduce large-scale epidemics.
In one aspect, the present invention provides novel peptidomimetic macrocycles that are useful in competitive binding assays to identify agents which bind to the natural ligand(s) of the proteins or peptides upon which the peptidomimetic macrocycles are modeled. For example, in the PB1/PA system, labeled peptidomimetic macrocycles based on PB1 can be used in a PA binding assay along with small molecules that competitively bind to PA. Competitive binding studies allow for rapid in vitro evaluation and determination of drug candidates specific for the PB1/PA system. Such binding studies can be performed with any of the peptidomimetic macrocycles disclosed herein and their binding partners.
In other aspects, the present invention provides for both prophylactic and therapeutic methods of treating a subject infected with, at risk of, or susceptible to an influenza virus. These methods comprise administering an effective amount of a compound to a warm blooded animal, including a human. In some embodiments, the administration of the compounds of the present invention prevents the proliferation or transmission of an influenza virus.
In some embodiments, peptidomimetic macrocycles are used to treat diseases induced by influenza viruses. Like other viruses, the replication of influenza virus involves six phases; transmission, entry, replication, biosynthesis, assembly, and exit. Entry occurs by endocytosis, replication and vRNP assembly takes place in the nucleus, and the virus buds from the plasma membrane. In the infected patient, the virus targets airway epithelial cells.
The methods described herein are also useful for development and/or identification of agents for the treatment of infections caused by viruses such as Abelson leukemia virus, Abelson murine leukemia virus, Abelson's virus, Acute laryngotracheobronchitis virus, Adelaide River virus, Adeno associated virus group, Adenovirus, African horse sickness virus, African swine fever virus, AIDS virus, Aleutian mink disease parvovirus, Alpharetrovirus, Alphavirus, ALV related virus, Amapari virus, Aphthovirus, Aquareovirus, Arbovirus, Arbovirus C, arbovirus group A, arbovirus group B, Arenavirus group, Argentine hemorrhagic fever virus, Argentine hemorrhagic fever virus, Arterivirus, Astrovirus, Ateline herpesvirus group, Aujezky's disease virus, Aura virus, Ausduk disease virus, Australian bat lyssavirus, Aviadenovirus, avian erythroblastosis virus, avian infectious bronchitis virus, avian leukemia virus, avian leukosis virus, avian lymphomatosis virus, avian myeloblastosis virus, avian paramyxovirus, avian pneumoencephalitis virus, avian reticuloendotheliosis virus, avian sarcoma virus, avian type C retrovirus group, Avihepadnavirus, Avipoxvirus, B virus, B19 virus, Babanki virus, baboon herpesvirus, baculovirus, Barmah Forest virus, Bebaru virus, Berrimah virus, Betaretrovirus, Birnavirus, Bittner virus, BK virus, Black Creek Canal virus, bluetongue virus, Bolivian hemorrhagic fever virus, Boma disease virus, border disease of sheep virus, borna virus, bovine alphaherpesvirus 1, bovine alphaherpesvirus 2, bovine coronavirus, bovine ephemeral fever virus, bovine immunodeficiency virus, bovine leukemia virus, bovine leukosis virus, bovine mammillitis virus, bovine papillomavirus, bovine papular stomatitis virus, bovine parvovirus, bovine syncytial virus, bovine type C oncovirus, bovine viral diarrhea virus, Buggy Creek virus, bullet shaped virus group, Bunyamwera virus supergroup, Bunyavirus, Burkitt's lymphoma virus, Bwamba Fever, CA virus, Calicivirus, California encephalitis virus, camelpox virus, canarypox virus, canid herpesvirus, canine coronavirus, canine distemper virus, canine herpesvirus, canine minute virus, canine parvovirus, Cano Delgadito virus, caprine arthritis virus, caprine encephalitis virus, Caprine Herpes Virus, Capripox virus, Cardiovirus, caviid herpesvirus 1, Cercopithecid herpesvirus 1, cercopithecine herpesvirus 1, Cercopithecine herpesvirus 2, Chandipura virus, Changuinola virus, channel catfish virus, Charleville virus, chickenpox virus, Chikungunya virus, chimpanzee herpesvirus, chub reovirus, chum salmon virus, Cocal virus, Coho salmon reovirus, coital exanthema virus, Colorado tick fever virus, Coltivirus, Columbia SK virus, common cold virus, contagious ecthyma virus, contagious pustular dermatitis virus, Coronavirus, Corriparta virus, coryza virus, cowpox virus, coxsackie virus, CPV (cytoplasmic polyhedrosis virus), cricket paralysis virus, Crimean-Congo hemorrhagic fever virus, croup associated virus, Cryptovirus, Cypovirus, Cytomegalovirus, cytomegalovirus group, cytoplasmic polyhedrosis virus, deer papillomavirus, deltaretrovirus, dengue virus, Densovirus, Dependovirus, Dhori virus, diploma virus, Drosophila C virus, duck hepatitis B virus, duck hepatitis virus 1, duck hepatitis virus 2, duovirus, Duvenhage virus, Deformed wing virus DWV, eastern equine encephalitis virus, eastern equine encephalomyelitis virus, EB virus, Ebola virus, Ebola-like virus, echo virus, echovirus, echovirus 10, echovirus 28, echovirus 9, ectromelia virus, EEE virus, EIA virus, EIA virus, encephalitis virus, encephalomyocarditis group virus, encephalomyocarditis virus, Enterovirus, enzyme elevating virus, enzyme elevating virus (LDH), epidemic hemorrhagic fever virus, epizootic hemorrhagic disease virus, Epstein-Barr virus, equid alphaherpesvirus 1, equid alphaherpesvirus 4, equid herpesvirus 2, equine abortion virus, equine arteritis virus, equine encephalosis virus, equine infectious anemia virus, equine morbillivirus, equine rhinopneumonitis virus, equine rhinovirus, Eubenangu virus, European elk papillomavirus, European swine fever virus, Everglades virus, Eyach virus, felid herpesvirus 1, feline calicivirus, feline fibrosarcoma virus, feline herpesvirus, feline immunodeficiency virus, feline infectious peritonitis virus, feline leukemia/sarcoma virus, feline leukemia virus, feline panleukopenia virus, feline parvovirus, feline sarcoma virus, feline syncytial virus, Filovirus, Flanders virus, Flavivirus, foot and mouth disease virus, Fort Morgan virus, Four Corners hantavirus, fowl adenovirus 1, fowlpox virus, Friend virus, Gammaretrovirus, GB hepatitis virus, GB virus, German measles virus, Getah virus, gibbon ape leukemia virus, glandular fever virus, goatpox virus, golden shinner virus, Gonometa virus, goose parvovirus, granulosis virus, Gross' virus, ground squirrel hepatitis B virus, group A arbovirus, Guanarito virus, guinea pig cytomegalovirus, guinea pig type C virus, Hantaan virus, Hantavirus, hard clam reovirus, hare fibroma virus, HCMV (human cytomegalovirus), hemadsorption virus 2, hemagglutinating virus of Japan, hemorrhagic fever virus, hendra virus, Henipaviruses, Hepadnavirus, hepatitis A virus, hepatitis B virus group, hepatitis C virus, hepatitis D virus, hepatitis delta virus, hepatitis E virus, hepatitis F virus, hepatitis G virus, hepatitis nonA nonB virus, hepatitis virus, hepatitis virus (nonhuman), hepatoencephalomyelitis reovirus 3, Hepatovirus, heron hepatitis B virus, herpes B virus, herpes simplex virus, herpes simplex virus 1, herpes simplex virus 2, herpesvirus, herpesvirus 7, Herpesvirus ateles, Herpesvirus hominis, Herpesvirus infection, Herpesvirus saimiri, Herpesvirus suis, Herpesvirus varicellae, Highlands J virus, Hirame rhabdovirus, hog cholera virus, human adenovirus 2, human alphaherpesvirus 1, human alphaherpesvirus 2, human alphaherpesvirus 3, human B lymphotropic virus, human betaherpesvirus 5, human coronavirus, human cytomegalovirus group, human foamy virus, human gammaherpesvirus 4, human gammaherpesvirus 6, human hepatitis A virus, human herpesvirus 1 group, human herpesvirus 2 group, human herpesvirus 3 group, human herpesvirus 4 group, human herpesvirus 6, human herpesvirus 8, human immunodeficiency virus, human immunodeficiency virus 1, human immunodeficiency virus 2, human papillomavirus, human T cell leukemia virus, human T cell leukemia virus I, human T cell leukemia virus II, human T cell leukemia virus III, human T cell lymphoma virus I, human T cell lymphoma virus II, human T cell lymphotropic virus type 1, human T cell lymphotropic virus type 2, human T lymphotropic virus I, human T lymphotropic virus II, human T lymphotropic virus III, Ichnovirus, infantile gastroenteritis virus, infectious bovine rhinotracheitis virus, infectious haematopoietic necrosis virus, infectious pancreatic necrosis virus, influenza virus A, influenza virus B, influenza virus C, influenza virus D, influenza virus pr8, insect iridescent virus, insect virus, iridovirus, Japanese B virus, Japanese encephalitis virus, JC virus, Junin virus, Kaposi's sarcoma-associated herpesvirus, Kemerovo virus, Kilham's rat virus, Klamath virus, Kolongo virus, Korean hemorrhagic fever virus, kumba virus, Kysanur forest disease virus, Kyzylagach virus, La Crosse virus, lactic dehydrogenase elevating virus, lactic dehydrogenase virus, Lagos bat virus, Langur virus, lapine parvovirus, Lassa fever virus, Lassa virus, latent rat virus, LCM virus, Leaky virus, Lentivirus, Leporipoxvirus, leukemia virus, leukovirus, lumpy skin disease virus, lymphadenopathy associated virus, Lymphocryptovirus, lymphocytic choriomeningitis virus, lymphoproliferative virus group, Machupo virus, mad itch virus, mammalian type B oncovirus group, mammalian type B retroviruses, mammalian type C retrovirus group, mammalian type D retroviruses, mammary tumor virus, Mapuera virus, Marburg virus, Marburg-like virus, Mason Pfizer monkey virus, Mastadenovirus, Canaro virus, ME virus, measles virus, Menangle virus, Mengo virus, Mengovirus, Middelburg virus, milkers nodule virus, mink enteritis virus, minute virus of mice, MLV related virus, MM virus, Mokola virus, Molluscipoxvirus, Molluscum contagiosum virus, monkey B virus, monkeypox virus, Mononegavirales, Morbillivirus, Mount Elgon bat virus, mouse cytomegalovirus, mouse encephalomyelitis virus, mouse hepatitis virus, mouse K virus, mouse leukemia virus, mouse mammary tumor virus, mouse minute virus, mouse pneumonia virus, mouse poliomyelitis virus, mouse polyomavirus, mouse sarcoma virus, mousepox virus, Mozambique virus, Mucambo virus, mucosal disease virus, mumps virus, murid betaherpesvirus 1, murid cytomegalovirus 2, murine cytomegalovirus group, murine encephalomyelitis virus, murine hepatitis virus, murine leukemia virus, murine nodule inducing virus, murine polyomavirus, murine sarcoma virus, Muromegalovirus, Murray Valley encephalitis virus, myxoma virus, Myxovirus, Myxovirus multiforme, Myxovirus parotitidis, Nairobi sheep disease virus, Nairovirus, Nanirnavirus, Nariva virus, Ndumo virus, Neethling virus, Nelson Bay virus, neurotropic virus, New World Arenavirus, newborn pneumonitis virus, Newcastle disease virus, Nipah virus, noncytopathogenic virus, Norwalk virus, nuclear polyhedrosis virus (NPV), nipple neck virus, O'nyong'nyong virus, Ockelbo virus, oncogenic virus, oncogenic viruslike particle, oncornavirus, Orbivirus, Orf virus, Oropouche virus, Orthohepadnavirus, Orthomyxovirus, Orthopoxvirus, Orthoreovirus, Orungo, ovine papillomavirus, ovine catarrhal fever virus, owl monkey herpesvirus, Palyam virus, Papillomavirus, Papillomavirus sylvilagi, Papovavirus, parainfluenza virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, parainfluenza virus type 4, Paramyxovirus, Parapoxvirus, paravaccinia virus, Parvovirus, Parvovirus B19, parvovirus group, Pestivirus, Phlebovirus, phocine distemper virus, Picodnavirus, Picornavirus, pig cytomegalovirus, pigeonpox virus, Piry virus, Pixuna virus, pneumonia virus of mice, Pneumovirus, poliomyelitis virus, poliovirus, Polydnavirus, polyhedral virus, polyoma virus, Polyomavirus, Polyomavirus bovis, Polyomavirus cercopitheci, Polyomavirus hominis 2, Polyomavirus maccacae 1, Polyomavirus muris 1, Polyomavirus muris 2, Polyomavirus papionis 1, Polyomavirus papionis 2, Polyomavirus sylvilagi, Pongine herpesvirus 1, porcine epidemic diarrhea virus, porcine hemagglutinating encephalomyelitis virus, porcine parvovirus, porcine transmissible gastroenteritis virus, porcine type C virus, pox virus, poxvirus, poxvirus variolae, Prospect Hill virus, Provirus, pseudocowpox virus, pseudorabies virus, psittacinepox virus, quailpox virus, rabbit fibroma virus, rabbit kidney vaculolating virus, rabbit papillomavirus, rabies virus, raccoon parvovirus, raccoonpox virus, Ranikhet virus, rat cytomegalovirus, rat parvovirus, rat virus, Rauscher's virus, recombinant vaccinia virus, recombinant virus, reovirus, reovirus 1, reovirus 2, reovirus 3, reptilian type C virus, respiratory infection virus, respiratory syncytial virus, respiratory virus, reticuloendotheliosis virus, Rhabdovirus, Rhabdovirus carpia, Rhadinovirus, Rhinovirus, Rhizidiovirus, Rift Valley fever virus, Riley's virus, rinderpest virus, RNA tumor virus, Ross River virus, Rotavirus, rougeole virus, Rous sarcoma virus, rubella virus, rubeola virus, Rubivirus, Russian autumn encephalitis virus, SA 11 simian virus, SA2 virus, Sabia virus, Sagiyama virus, Saimirine herpesvirus 1, salivary gland virus, sandfly fever virus group, Sandjimba virus, SARS virus, SDAV (sialodacryoadenitis virus), sealpox virus, Semliki Forest Virus, Seoul virus, sheeppox virus, Shope fibroma virus, Shope papilloma virus, simian foamy virus, simian hepatitis A virus, simian human immunodeficiency virus, simian immunodeficiency virus, simian parainfluenza virus, simian T cell lymphotrophic virus, simian virus, simian virus 40, Simplexvirus, Sin Nombre virus, Sindbis virus, smallpox virus, South American hemorrhagic fever viruses, sparrowpox virus, Spumavirus, squirrel fibroma virus, squirrel monkey retrovirus, SSV 1 virus group, STLV (simian T lymphotropic virus) type I, STLV (simian T lymphotropic virus) type II, STLV (simian T lymphotropic virus) type III, stomatitis papulosa virus, submaxillary virus, suid alphaherpesvirus 1, suid herpesvirus 2, Suipoxvirus, swamp fever virus, swinepox virus, Swiss mouse leukemia virus, TAC virus, Tacaribe complex virus, Tacaribe virus, Tanapox virus, Taterapox virus, Tench reovirus, Theiler's encephalomyelitis virus, Theiler's virus, Thogoto virus, Thottapalayam virus, Tick borne encephalitis virus, Tioman virus, Togavirus, Torovirus, tumor virus, Tupaia virus, turkey rhinotracheitis virus, turkeypox virus, type C retroviruses, type D oncovirus, type D retrovirus group, ulcerative disease rhabdovirus, Una virus, Uukuniemi virus group, vaccinia virus, vacuolating virus, varicella zoster virus, Varicellovirus, Varicola virus, variola major virus, variola virus, Vasin Gishu disease virus, VEE virus, Venezuelan equine encephalitis virus, Venezuelan equine encephalomyelitis virus, Venezuelan hemorrhagic fever virus, vesicular stomatitis virus, Vesiculovirus, Vilyuisk virus, viper retrovirus, viral haemorrhagic septicemia virus, Visna Maedi virus, Visna virus, volepox virus, VSV (vesicular stomatitis virus), Wallal virus, Warrego virus, wart virus, WEE virus, West Nile virus, western equine encephalitis virus, western equine encephalomyelitis virus, Whataroa virus, Winter Vomiting Virus, woodchuck hepatitis B virus, woolly monkey sarcoma virus, wound tumor virus, WRSV virus, Yaba monkey tumor virus, Yaba virus, Yatapoxvirus, yellow fever virus, and the Yug Bogdanovac virus. In one embodiment an infectome will be produced for each virus that includes an inventory of the host cellular genes involved in virus infection during a specific phase of viral infection, such cellular entry or the replication cycle.
For some viruses a great deal of progress has been made in the elucidation of the steps involved during infection of host cells, and any of these steps can be targeted using peptidomimetic macrocycles. For example, experiments initiated in the early 1980s showed that influenza virus follows a stepwise, endocytic entry program with elements shared with other viruses such as alpha-and rhabdoviruses (Marsh and Helenius 1989; Whittaker 2006). The steps include: 1) Initial attachment to sialic acid containing glycoconjugates receptors on the cell surface; 2) signaling induced by the virus particle; 3) endocytosis by clathrin-dependent and clathrin-independent cellular mechanism; 4) acid-induced, hemaglutinin (HA)-mediated penetration from late endosomes; 5) acid-activated, M2 and matrix protein (M1) dependent uncoating of the capsid; and, 6) intra-cytosolic transport and nuclear import of vRNPs. These steps depend on assistance from the host cell in the form of sorting receptors, vesicle formation machinery, kinase-mediated regulation, organelle acidification, and, most likely, activities of the cytoskeleton.
Influenza attachment to the cells surface occurs via binding of the HA1 subunit to cell surface glycoproteins and glycolipids that carry oligosaccharide moieties with terminal sialic acid residues (Skehel and Wiley 2000). The linkage by which the sialic acid is connected to the next saccharide contributes to species specificity. Avian strains including H5N1 prefer an α-(2,3)-link and human strains α-(2,6)-link (Matrosovich 2006). In epithelial cells, binding occurs preferentially to microvilli on the apical surface, and endocytosis occurs at base of these extensions (Matlin 1982). Whether receptor binding induces signals that prepare the cell for the invasion is not yet known, but it is likely because activation of protein kinase C and synthesis of phopshatidylinositol-3-phosphate (PI3P) are required for efficient entry (Sieczkarski et al. 2003; Whittaker 2006).
Endocytic internalization occurs within a few minutes after binding (Matlin 1982; Yoshimura and Ohnishi 1984). In tissue culture cells influenza virus makes use of three different types of cellular processes; 1) preexisting clathrin coated pits, 2) virus-induced clathrin coated pits, and 3) endocytosis in vesicles without visible coat (Matlin 1982; Sieczkarski and Whittaker 2002; Rust et al. 2004). Video microscopy using fluorescent viruses showed the virus particles undergoing actin-mediated rapid motion in the cell periphery followed by minus end-directed, microtubule-mediated transport to the perinuclear area of the cell. Live cell imaging indicated that the virus particles first entered a subpopulation of mobile, peripheral early endosomes that carry them deeper into the cytoplasm before penetration takes place (Lakadamyali et al. 2003; Rust et al. 2004). The endocytotic process is regulated by protein and lipid kinases, the proteasome, as well as by Rabs and ubiquitin-dependent sorting factors (Khor et al. 2003; Whittaker 2006).
The membrane penetration step is mediated by low pH-mediated activation of the trimeric, metastable HA, and the conversion of this Type I viral fusion protein to a membrane fusion competent conformation (Maeda et al. 1981; White et al. 1982). This occurs about 16 min after internalization, and the pH threshold varies between strains in the 5.0-5.6 range. The target membrane is the limiting membrane of intermediate or late endosomes.
The mechanism of fusion has been extensively studied (Kielian and Rey 2006). Further it was observed that fusion itself does not seem to require any host cell components except a lipid bilayer membrane and a functional acidification system (Maeda et al. 1981; White et al. 1982). The penetration step is inhibited by agents such as lysosomotropic weak bases, carboxylic ionophores, and proton pump inhibitors (Matlin 1982; Whittaker 2006).
To allow nuclear import of the incoming vRNPs, the capsid has to be disassembled. This step involves acidification of the viral interior through the amantadine-sensitive M2-channels causes dissociation of M1 from the vRNPs (Bukrinskaya et al. 1982; Martin and Helenius 1991; Pinto et al. 1992). Transport of the individual vRNPs to the nuclear pore complexes and transfer into the nucleus depends on cellular nuclear transport receptors (O'Neill et al. 1995; Cros et al. 2005). Replication of the viral RNAs (synthesis of positive and negative strands), and transcription occurs in complexes tightly associated with the chromatin in the nucleus. It is evident that, although many of the steps are catalyzed by the viral polymerase, cellular factors are involved including RNA polymerase activating factors, a chaperone HSP90, hCLE, and a human splicing factor UAP56. Viral gene expression is subject to complex cellular control at the transcriptional level, a control system dependent on cellular kinases (Whittaker 2006).
The final assembly of an influenza particle occurs during a budding process at the plasma membrane. In epithelial cells, budding occurs at the apical membrane domain only (Rodriguez-Boulan 1983). First, the progeny vRNPs are transported within the nucleoplasm to the nuclear envelope, then from the nucleus to the cytoplasm, and finally they accumulate in the cell periphery. Exit from the nucleus is dependent on viral protein NEP and M1, and a variety of cellular proteins including CRM1 (a nuclear export receptor), caspases, and possibly some nuclear protein chaperones. Phosphorylation plays a role in nuclear export by regulating M1 and NEP synthesis, and also through the MAPK/ERK system (Bui et al. 1996; Ludwig 2006). G protein and protein kinase signaling is involved in influenza virus budding from infected host cells (Hui E. and Nayak D, 2002).
The three membrane proteins of the virus are synthesized, folded and assembled into oligomers in the ER (Doms et al. 1993). They pass through the Golgi complex; undergo maturation through modification of their carbohydrate moieties and proteolytic cleavage. After reaching the plasma membrane they associate with M1 and the vRNPs in a budding process that result in the inclusion of all eight vRNPs and exclusion of most host cell components except lipids.
Influenza infection is associated with activation of several signaling cascades including the MAPK pathway (ERK, JNK, p38 and BMK-1/ERK5), the KB/NF-κB signaling module, the Raf/MEK/ERK cascade, and programmed cell death (Ludwig 2006). These result in a variety of effects that limit the progress of infection such as transcriptional activation of IFN-β, apoptotic cell death, and a block in virus escape of from late endosomes (Ludwig 2006).

Administration

The aqueous pharmaceutical formulations of the present disclosure can draw upon many suitable parenteral modes of administration route. The formulations can be, for example, administered intravenously, intraarterially, intrathecally, or subcutaneously. If combinations of agents are administered as separate formulations, they can be administered by the same route or by different routes.
In some embodiments, the aqueous pharmaceutical formulation is administered in a single dose. A single dose of the aqueous pharmaceutical formulation can also be used when it is co-administered with another substance (e.g., an analgesic) for treatment of an acute condition.
In some embodiments, the aqueous pharmaceutical formulation (by itself or in combination with other drugs) is administered in multiple doses. Dosing can be about once, twice, three times, four times, five times, six times, seven times, eight times, nine times, ten times or more than ten times per day. Dosing can be about once a year, twice a year, every six months, every 4 months, every 3 months, every 60 days, once a month, once every two weeks, once a week, or once every other day. In another embodiment the aqueous pharmaceutical formulation alone or in combination with another therapeutic substance is administered together about once per day to about 10 times per day. In another embodiment the administration of the aqueous pharmaceutical formulation alone or in combination with another therapeutic substance continues for less than about 7 days. In yet another embodiment the administration continues for more than about 6, 10, 14, 28 days, two months, six months, or one year.
Administration of the formulations of the disclosure can continue as long as necessary. In some embodiments, a aqueous pharmaceutical formulation of the disclosure is administered for more than 1, 2, 3, 4, 5, 6, 7, 14, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 105, 112, 119, 126, 133, or 140 days. In some embodiments, a aqueous pharmaceutical formulation of the disclosure is administered for less than 140, 133, 126, 119, 112, 105, 98, 91, 84, 77, 70, 63, 56, 49, 42, 35, 28, 14, 7, 6, 5, 4, 3, 2, or 1 day. In some embodiments, an aqueous pharmaceutical formulation of the disclosure is administered for more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 days. In some embodiments, a aqueous pharmaceutical formulation of the disclosure is administered for less than 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 months. In some embodiments, an aqueous pharmaceutical formulation of the disclosure is administered for more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 years. In some embodiments, a aqueous pharmaceutical formulation of the disclosure is administered for less than 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years. In some embodiments, a aqueous pharmaceutical formulation of the disclosure is administered chronically on an ongoing basis.
Dosing for the aqueous pharmaceutical formulation formulations of the disclosure can be found by routine experimentation. The daily dose can range from about 1×10⁻⁸g to 5000 mg. Daily dose range can depend on the form of the aqueous pharmaceutical formulation e.g., the peptidomimetic macrocycle used, and/or route of administration, as described herein. For example, daily dose can be in the range of about 0.1-5000 mg, about 0.1-3000 mg, about 0.1-2000 mg, about 0.1-1000 mg, about 01.-500 mg, about 0.1-100 mg, 1-5000 mg, about 1-3000 mg, about 1-2000 mg, about 1-1000 mg, about 1-500 mg, or about 1-100 mg, about 10-5000 mg, about 10-3000 mg, about 10-2000 mg, about 10-1000 mg, about 10-500 mg, about 10-200 mg, about 10-100 mg, about 20-2000 mg, about 20-1500 mg, about 20-1000 mg, about 20-500 mg, about 20-100 mg, about 50-5000 mg, about 50-4000 mg, about 50-3000 mg, about 50-2000 mg, about 50-1000 mg, about 50-500 mg, about 50-100 mg, about 100-5000 mg, about 100-4000 mg, about 100-3000 mg, about 100-2000 mg, about 100-1000 mg, about 100-500 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is about 0.01, 0.1, 1, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is 0.01 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is 0.1 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is 1 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is up to 10 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is up to 20 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is 50 mg. In some embodiments, the daily dose of the aqueous pharmaceutical formulation is 100 mg.

III. Kits

For use in the therapeutic methods of use described herein, the formulations of the disclosure can be available as a kit. Such kits can include a carrier, package, or container that is optionally compartmentalized to receive one or more doses of the aqueous pharmaceutical formulations for use in a method described herein. The kits provided herein can contain packaging materials. Packaging materials for use in packaging pharmaceutical products include, but are not limited to those described in e.g., U.S. Pat. No. 5,323,907. Examples of pharmaceutical packaging materials include, but are not limited to, blister packs, bottles, tubes, bags, containers, bottles, and any packaging material suitable for a selected formulation and intended mode of administration and treatment.
The aqueous pharmaceutical formulations of the disclosure can be packaged in multidose form or in single dose form. In some cases, the formulations are packaged in multidose forms. In some embodiments the formulations are packaged as single dose units. In some embodiments of the disclosure single dose packaging of the formulations can offer several advantages over multi dose packaging including dosage control, increased patient compliance, improved product labeling, and reduced counterfeiting. In various embodiments single dosage packaging of the formulations of the disclosure can be in form of vials, ampoules, tubes, bottles, pouches, packettes, syringes or blister packs. In some embodiments the single dose containers can be grouped together and placed into additional containers. In some embodiments the secondary container is a pouch.
In some examples, the formulations of the disclosure can be packed in a bottle or a vial. In some examples, the formulations can be packed in glass serum vial. In some examples, the formulations can be packed in serum vials composed of borosilicate glass. In some examples, the formulations are packed in a 1 mL, a 2 mL, a 3 mL, a 4 mL, a 5 mL, a 10 mL, a 20 mL, a 30 mL, or a 50 mL glass vial. In some examples, the formulations are packed in a 5 mL glass vial. In some examples, the formulations are packed in a 10 mL glass vial. In some examples, the formulations are packed in a 15 mL glass vial. In some examples, the formulations are packed in a 20 mL glass vial. In some embodiments, the vials comprise a 5 mm, a 10 mm, a 15 mm, 20 mm, 30 mm, or 50 mm orifice. In some embodiments, the formulations are packed in a 5 mL borosilicate glass vial with a 20 mm orifice. In some embodiments, the formulations are packed in a 10 mL borosilicate glass vial with a 20 mm orifice. The containers, bottles and/or vials can be equipped with suitable caps or stoppers. In some embodiments, the vials are equipped with a vinyl stopper. In some embodiments the formulations are packed in a 10 mL glass vial, with a 20 mm orifice, equipped with vinyl stoppers. The stoppers can be coated with FluroTek®. The containers, bottles and/or can also be equipped with a seal, for example, crimped-on flip-off caps. The seal can be aluminum and/or plastic. The container can be a glass ampoule.
In some embodiments, the containers, including the vials and the bottles, can be inspected for visible particulates, glass defects, and/or stopper/cap integrity before packaging the formulations therein. In some embodiments, the containers, including the vials and the bottles, can be inspected for visible particulates, glass defects, and/or stopper/cap integrity after packaging the formulations therein. In some embodiments, the containers, including the vials and the bottles, can be inspected for visible particulates, glass defects, and/or stopper/cap integrity before and/or after packaging the formulations therein. The containers, including the vials and the bottles, can also be additionally inspected for fill height after packaging the formulations therein. The inspection can be visual inspections and can be carried out under any convenient condition, for example in front of a black and white background.
A kit can also include labels listing contents and/or instructions for use, and package inserts with instructions for use. A set of instructions will also typically be included. In one embodiment, a label is on or associated with the container. In one embodiment, a label is on a container when letters, numbers or other characters forming the label are attached, molded or etched into the container itself; a label is associated with a container when it is present within a receptacle or carrier that also holds the container, e.g., as a package insert. In one embodiment, a label is used to indicate that the contents are to be used for a specific therapeutic application. The label also indicates directions for use of the contents, such as in the methods described herein. The labels can optionally indicate one or more items selected from a group comprising the date of manufacturing of the formulation, the recommended storage conditions, intended mode of administration for the formulation, the amount of formulation enclosed and/or the concentration of the peptidomimetic macrocycle. The labels can further include any applicable warnings and/or possible side effects.
In certain embodiments, the pharmaceutical formulations are presented in a pack or dispenser device which contains one or more unit dosage forms containing a formulation provided herein. The pack, for example, contains metal or plastic foil, such as a blister pack. In one embodiment, the pack or dispenser device is accompanied by instructions for administration. In one embodiment, the pack or dispenser is also accompanied with a notice associated with the container in form prescribed by a governmental agency regulating the manufacture, use, or sale of pharmaceuticals, which notice is reflective of approval by the agency of the form of the drug for human or veterinary administration. Such notice, for example, is the labeling approved by the U.S. Food and Drug Administration for prescription drugs, or the approved product insert. In one embodiment, formulations containing a compound provided herein formulated in a compatible pharmaceutical carrier are also prepared, placed in an appropriate container, and labeled for treatment of an indicated condition.

EXAMPLES

Example 1: Peptidomimetic Macrocycles

Peptidomimetic macrocycles were synthesized, purified and analyzed as previously described and as described below (Schafmeister et al., J. Am. Chem. Soc. 122:5891-5892 (2000); Schafmeister & Verdine, J. Am. Chem. Soc. 122:5891 (2005); Walensky et al., Science 305:1466-1470 (2004); and U.S. Pat. No. 7,192,713). Peptidomimetic macrocycles were designed by replacing two or more naturally occurring amino acids with the corresponding synthetic amino acids. Substitutions were made at i and i+4, and i and i+7 positions. Peptide synthesis was performed either manually or on an automated peptide synthesizer (Applied Biosystems, model 433A), using solid phase conditions, rink amide AM resin (Novabiochem), and Fmoc main-chain protecting group chemistry. For the coupling of natural Fmoc-protected amino acids (Novabiochem), 10 equivalents of amino acid and a 1:1:2 molar ratio of coupling reagents HBTU/HOBt (Novabiochem)/DIEA were employed. Non-natural amino acids (4 equiv) were coupled with a 1:1:2 molar ratio of HATU (Applied Biosystems)/HOBt/DIEA. The N-termini of the synthetic peptides were acetylated, while the C-termini were amidated.
Purification of cross-linked compounds was achieved by high performance liquid chromatography (HPLC) (Varian ProStar) on a reverse phase C18 column (Varian) to yield the pure compounds. Chemical composition of the pure products was confirmed by LC/MS mass spectrometry (Micromass LCT interfaced with Agilent 1100 HPLC system) and amino acid analysis (Applied Biosystems, model 420A).
The following protocol was used in the synthesis of dialkyne-crosslinked peptidomimetic macrocycles, including SP662, SP663 and SP664. Fully protected resin-bound peptides were synthesized on a PEG-PS resin (loading 0.45 mmol/g) on a 0.2 mmol scale. Deprotection of the temporary Fmoc group was achieved by 3×10 min treatments of the resin bound peptide with 20% (v/v) piperidine in DMF. After washing with NMP (3×), dichloromethane (3×) and NMP (3×), coupling of each successive amino acid was achieved with 1×60 min incubation with the appropriate preactivated Fmoc-amino acid derivative. All protected amino acids (0.4 mmol) were dissolved in NMP and activated with HCTU (0.4 mmol) and DIEA (0.8 mmol) prior to transfer of the coupling solution to the deprotected resin-bound peptide. After coupling was completed, the resin was washed in preparation for the next deprotection/coupling cycle. Acetylation of the amino terminus was carried out in the presence of acetic anhydride/DIEA in NMP. The LC-MS analysis of a cleaved and deprotected sample obtained from an aliquot of the fully assembled resin-bound peptide was accomplished in order to verifying the completion of each coupling. In a typical example, tetrahydrofuran (4 ml) and triethylamine (2 ml) were added to the peptide resin (0.2 mmol) in a 40 ml glass vial and shaken for 10 minutes. Pd(PPh₃)₂Cl₂(0.014 g, 0.02 mmol) and copper iodide (0.008 g, 0.04 mmol) were then added and the resulting reaction mixture was mechanically shaken 16 hours while open to atmosphere. The diyne-cyclized resin-bound peptides were deprotected and cleaved from the solid support by treatment with TFA/H₂O/TIS (95/5/5 v/v) for 2.5 h at room temperature. After filtration of the resin the TFA solution was precipitated in cold diethyl ether and centrifuged to yield the desired product as a solid. The crude product was purified by preparative HPLC.
The following protocol was used in the synthesis of single alkyne-crosslinked peptidomimetic macrocycles, including SP665. Fully protected resin-bound peptides were synthesized on a Rink amide MBHA resin (loading 0.62 mmol/g) on a 0.1 mmol scale. Deprotection of the temporary Fmoc group was achieved by 2×20 min treatments of the resin bound peptide with 25% (v/v) piperidine in NMP. After extensive flow washing with NMP and dichloromethane, coupling of each successive amino acid was achieved with 1×60 min incubation with the appropriate preactivated Fmoc-amino acid derivative. All protected amino acids (1 mmol) were dissolved in NMP and activated with HCTU (1 mmol) and DIEA (1 mmol) prior to transfer of the coupling solution to the deprotected resin-bound peptide. After coupling was completed, the resin was extensively flow washed in preparation for the next deprotection/coupling cycle. Acetylation of the amino terminus was carried out in the presence of acetic anhydride/DIEA in NMP/NMM. The LC-MS analysis of a cleaved and deprotected sample obtained from an aliquot of the fully assembled resin-bound peptide was accomplished in order to verifying the completion of each coupling. In a typical example, the peptide resin (0.1 mmol) was washed with DCM. Resin was loaded into a microwave vial. The vessel was evacuated and purged with nitrogen. Molybdenumhexacarbonyl (0.01 eq, Sigma Aldrich 199959) was added. Anhydrous chlorobenzene was added to the reaction vessel. Then 2-fluorophenol (1 eq, Sigma Aldrich F12804) was added. The reaction was then loaded into the microwave and held at 130° C. for 10 minutes. Reaction can need to be pushed a subsequent time for completion. The alkyne metathesized resin-bound peptides were deprotected and cleaved from the solid support by treatment with TFA/H₂O/TIS (94/3/3 v/v) for 3 h at room temperature. After filtration of the resin the TFA solution was precipitated in cold diethyl ether and centrifuged to yield the desired product as a solid. The crude product was purified by preparative HPLC.
Table 1 shows a list of peptidomimetic macrocycles that were prepared. Table 1a, Table 1b, Table 1c and Table 1d shows a selection of peptidomimetic macrocycles. In some embodiments, peptidomimetic macrocycles exclude peptidomimetic macrocycles shown in Table 2a. In some embodiments, peptidomimetic macrocycles do not comprise a peptidomimetic macrocycle structure as shown in Table 2a. In other embodiments, peptidomimetic macrocycles exclude peptidomimetic macrocycles shown in Table 2b. In some embodiments, the peptidomimetic macrocycles disclosed herein do not comprise a peptidomimetic macrocycle structure as shown in Table 2b.

Example 2: Preparation of a Pharmaceutical Formulation 1-Liter Peptidomimetic Formulation Batch

Aileron peptide 1 is formulated as a pharmaceutical formulation. Aileron peptide 1 is an alpha helical hydrocarbon cross-linked polypeptide macrocycle, with an amino acid sequence less than 20 amino acids long that is derived from the transactivation domain of wild type human P53 protein and that contains a phenylalanine, a tryptophan and a leucine amino acid in the same positions relative to each other as in the transactivation domain of wild type human P53 protein. Aileron peptide 1 has a single cross link spanning amino acids in the i to the i+7 position of the amino acid sequence and has more than three amino acids between the i+7 position and the carboxyl terminus. Aileron peptide 1 binds to human MDM2 and MDM4 and has an observed mass of 950-975 m/e as measured by electrospray ionization-mass spectrometry.
For each liter of formulated Aileron peptide 1the peptides is sequentially dissolve in 900 mL of water for injection 182 mg monosodium phosphate, monohydrate, 2,968 mg disodium phosphate, anhydrous, and 82.2 g of D-trehalose. Add 3.0 mL of a 10% (w/w) aqueous solution of Polysorbate 20. Slowly add 15,000 mg Aileron peptide 1 divided by peptide content divided by peptide purity to the solution under stirring. E.g., if the peptide content is 94.3% and the peptide purity is 98.2%, 15,000/94.3*100/98./100 or 16,215 mg of bulk-Aileron peptide 1 would have to be added. While the peptide is dissolving the pH of the solution is kept between 7.5 and 7.7 by the addition of 0.1 N sodium hydroxide.
After all peptide is dissolved, adjust pH of the solution to 7.5±0.1 with sodium hydroxide and subsequently q.s. with WFI to 1,000 mL. Stir solution for 5 minutes and then clarify solution by passing it through a 0.22-μm PVDF-membrane filter.

Example 3: Sterile Filtration and Fill

The formulated product is filtered through two serial sterilization 0.22 μm PVDF membrane filters into a sterile container that is equipped with the fill needle. The filling process starts after both filters have passed the post filtration filter integrity test. If one or both filters do not pass the post filtration integrity test, the tandem sterile filtration process is repeated until both filters pass the test.
All vials are inspected for visible particulates, glass faults, fill high and stopper/cap integrity in front of a white and black background. Approximately 180 vial containers are then filled per 1-liter batch to a level of 5.2 mL to 5.7 mL each, with a fill target of 5.5 mL (the label claim is 5.0 mL). Fill volume accuracy is verified throughout the fill process. The filling machine loaded with vials and stoppers immediately stoppers each vial after it is filled. Capping occurs in line with filling and stopping or can occur separately under ISO Class 5 supply air. Weight check of the contents of the filled vials is performed throughout the filling process to assure that the vials receive the specified fill volume. Any rejected vials are discarded.

Example 4: Stability Analysis

To render the stability study more challenging 2 mL vials with 13 mm 0 stoppers and a fill volume of 1.0 mL were selected. The smaller vial size provided a greater surface to volume ratio which would amplify any container/closure effects on product stability. To assure that all surfaces of vial were challenged, the vials were stored in an inverted position. The tested storage conditions on inverted vials are: −20° C., +5° C., +25° C., and +40° C. RH.
The results of this study are depicted in Table 5. There is no appreciable purity loss at storage temperatures between −20° C. and +25° C. over the 6-month test period and only about a 1.8% purity loss of the sample that is stored at 40° C. over the same period. The observed small but continuous increase of RRT values between 0.22 and 0.81 in the 40° C. samples attests to both, the excellent detecting power and stability-indicating capability of the RP-HPLC (TFA) method. The peptidomimetic macrocycle concentration stayed within an acceptable ±4% range over the 6-month period for all samples independent of the storage temperature.

TABLE 5

Product stability results of Aileron peptide 1 formulated at 15 mg/mL in 20 mM sodium
phosphate, 240 mM trehalose, 300 ppm Polysorbate 20 buffer in a 5-fold scaled-down, inverted container/closure
configuration to amplify any potential product degrading effects by the container/closure surface.

Time Points

Initial

1 Month

2 Months

Storage	−20° C.	−20° C.	5° C.	25° C.	40° C.	−20° C.	5° C.	25° C.	40° C.

Assay [%]		100%	97%	96%	99%	103%	104%	101%	102%
Purity [%]	95.5%	95.9%	95.8%	96.1%	96.0%	95.8%	96.2%	96.1%	95.6%

Individual Impurities >0.1%

~RRT 0.22									0.08%
~RRT 0.32									0.09%
~RRT 0.47									0.08%
~RRT 0.81									0.08%
~RRT 0.84	0.11%				0.06%				0.13%
~RRT 0.88	0.17%	0.12%	0.12%	0.13%	0.13%	0.14%		0.14%	0.14%
~RRT 0.90	0.22%	0.16%	0.19%	0.18%	0.18%	0.16%	0.15%	0.20%	0.20%
~RRT 0.93	0.54%	0.47%	0.50%			0.51%	0.44%
~RRT 1.03	2.24%	2.21%	2.22%	2.20%	2.20%	2.18%	2.13%	2.27%	2.27%
~RRT 1.04	0.17%	0.11%	0.12%	0.17%	0.17%	0.15%	0.13%	ND	ND
~RRT 1.06				0.10%	0.10%			0.14%	0.14%
~RRT 1.07	0.12%	0.13%	0.15%	0.16%	0.16%	0.11%	0.07%	0.14%	0.14%
~RRT 1.10	0.95%	0.95%	0.93%	0.93%	0.99%	0.98%	0.89%	1.00%	1.06%

Particulate Matter [Number of particles per container)

<10 μm	<1	<1	<1	<1	<1
≥10 μm	140	38	23	28	31
≥25 μm	33	7	6	5	3
≥50 μm	4	2	3	2	0

Time Points

3 Months

6 Months

Storage	−20° C.	5° C.	25° C.	40° C.	−20° C.	5° C.	25° C.	40° C.

Assay [%]	99%	99%	99%	97%	100%	99%	99%	96%
Purity [%]	95.9%	95.8%	96.0%	95.2%	95.7%	95.5%	95.7%	93.9%

Individual Impurities >0.1%

~RRT 0.22				0.08%				0.16%
~RRT 0.32				0.14%				0.33%
~RRT 0.47				0.14%				0.25%
~RRT 0.81				0.12%				0.25%
~RRT 0.84				0.14%				0.25%
~RRT 0.88			0.13%	0.13%	0.14%	0.17%	0.09%	0.18%
~RRT 0.90	0.18%	0.17%	0.21%	0.21%	0.19%	0.21%	0.20%	0.23%
~RRT 0.93	0.50%	0.51%			0.55%	0.54%	0.49%	0.52%
~RRT 1.03	2.23%	2.24%	2.40%	2.40%	2.15%	2.24%	2.21%	2.30%
~RRT 1.04	0.14%	0.14%			0.19%	0.15%	0.12%	0.16%
~RRT 1.06	0.00%	0.00%	0.16%	0.16%	0.09%	0.09%	0.13%	0.13%
~RRT 1.07	0.15%	0.15%	0.17%	0.17%	0.12%	0.18%	0.12%	0.16%
~RRT 1.10	0.95%	0.96%	0.99%	1.08%	0.92%	0.97%	0.99%	1.20%

Particulate Matter [Number of particles per container)

<10 μm	<2	<1	<1	~8
≥10 μm	190	17	47	83
≥25 μm	20	2	7	14
≥50 μm	2	2	0	6

RRT = reative retention time.

Example 5: Comparative Data for TRIS and Phosphate Buffers

Composition of Formulations
Two formulations, F1 and F2 were formulated. Table 6 shows the compositions of the two formulations. Formulations were filled into 6 mL, Ø 20 mm, colorless vials. The vials were equipped with teflon serum-stoppers D777-1, Ø 20 mm and aluminum caps without PP-cap, Ø 20 mm. 6 vials of each formulation were prepared. The vials were stored at 2-8° C. Exposure to direct sunlight was avoided.

TABLE 6

Composition of formulations F1 and F2:

	Formu-
Peptidomimetic	lation			Surfactant	Peptide
Macrocycle	Code	Buffer system	Excipient	%	(mg/ml)

Aileron	F1		20 mM	240 mM	0.03	15
peptide 1		Na-Phosphate	Trehalose
		pH 7.5
	F2	20 mM		0.03
		Tris pH 7.5

Two placebo formulations P1 (with 20 mM Na-phosphate buffer) and P2 (with 20 mM Tris buffer), without the peptidomimetic macrocycle, were also prepared.
Every filled 6 ml vial was visually inspected using Seidenader. The results of this observation are summarized in Table 7. The observation images are shown in FIG. 2.

TABLE 7

Comparative visual inspection of F1, F2, P1 and
P2 indicate that the resulting formulations were
comparable and yielded minimal visible particles.

Form.

total filled/

no. of vials with particles

Code	inspected	0	1-5	6-10	>10	notes

F1
	6	4	2			1× faster
						1× particle
F2
	6	6
P1	6	5	1			1× faster
P2
	6	6

Example 6: Shelf Life of the Peptidomimetic Macrocycles of the Invention

A pharmaceutical formulation of Aileron peptide 1, was formulated as described above and stored at varying temperatures (−20° C., 2-8° C., 25° C. 60% humidity and 40° C. 75% humidity. The purity of the samples was analyzed at regular time intervals. The results of these experiments for Aileron peptide 1 are summarized in FIG. 4. These experiments support a greater than 2 year shelf life at −20° C. and 2-8° C.

Example 7

Stability testing of Aileron peptide 1 was performed on a pharmaceutical formulation formulated as described above and stored at varying temperatures (−20° C., 2-8° C., 25° C. 60% humidity and 40° C. 75% humidity. The purity of the samples was analyzed at regular time intervals. The results of these experiments for Aileron peptide 1 are summarized in Tables 8, 9, 10, and 11.

TABLE 8

	Storage: −20 ° C.

Initial

	1 Month	2 Months	3 Months	6 Months	9 Months	12 Months

Appearance¹	Conforms	Conforms	Conforms	Conforms	Conforms	Conforms	Conforms
pH²	7.6	7.6	7.6	7.6	7.6	7.6*	7.6
Assay	15.6	15.6	15.9	15.6	15.4	15.5	15.6
[mg/mL]
Purity [%]³	99.0	99.0	98.9	98.9	98.9	99.0	99.0
Impurities	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.88	0.4% @ 0.88
[%]⁴	0.4% @ 0.91	0.4% @ 0.91	0.5% @ 0.91	0.5% @ 0.91	0.5% @ 0.91	0.4% @ 0.91	0.4% @ 0.91
	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02
Particulate	184				18		557
Matter⁵
CCIT⁶	63				0		8
Endotoxin⁷	conforms						conforms
	<1.8						<1.6
	EU/mL						EU/mL

TABLE 9

	Storage: +5° C.

Initial

	1 Month	2 Months	3 Months	6 Months	9 Months	12 Months

Appearance¹	Conforms	Conforms	Conforms	Conforms	Conforms	Conforms	Conforms
pH²	7.6	7.6	7.6	7.7	7.7	7.6	7.6
Assay	15.6	15.7	15.8	15.8	15.5	15.6	15.6
[mg/mL]
Purity [%]³	99.0	99.0	99.0	99.0	98.9	99.0	99.0
Impurities	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89
[%]⁴	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.5% @ 0.91	0.4% @ 0.91	0.4% @ 0.91
	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02
Particulate	184				37		171
Matter⁵
CCIT⁶	63				4		11
Endotoxin⁷	conforms						conforms
	<1.8						<1.6
	EU/mL						EU/mL

TABLE 10

	Storage: +25° C./60% RH

	Initial	1 Month	2 Months	3 Months	6 Months	9 Months	12 Months

Appearance¹	Conforms	Conforms	Conforms	Conforms	Conforms	Conforms	Conforms
pH²	7.6	7.65	7.6	7.6	7.7	7.6	7.6
Assay	15.6	15.6	15.8	15.4	15.2	15.1	15.0
[mg/mL]
Purity [%]³	99.0	99.0	98.9	98.7	98.4	98.4	97.9
				0.2% @ 0.23	0.3% @ 0.23	0.4% @ 0.23	0.5% @ 0.23
Impurities [%]⁴							0.2% @ 0.32
				0.1% @ 0.82			0.4 @ 0.82
	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.88
	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91
	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02
Particulate	184				5		185
Matter⁵
CCIT⁶	63				1		8
Endotoxin⁷	conforms						conforms
	<1.8						<1.6
	EU/mL						EU/mL

¹Visual appearance Specification: Upon thawing, clear, colorless, particulate-free solution
²pH Specification: 7.3 to 7.7
³Purity by RP-HPLC(TFA) Specification: ≥95%; no single impurity >3%
⁴>0.1% Impurities listed by RRT
⁵USP <788> (light obstruction) Specification: ≤6,000 particles ≥10 μm and ≤600 particles ≥25 μm per vial (upper cell: ≥10 μm particles; lower cells: ≥25 μm particles
⁶CCIT Specification: No dye intrusion
⁷Endotoxin: ≤4.4 EU/mL (based upon maximum patient dose of 17 mg ALRN-6924 per Kg of patient weight)

TABLE 11

	Storage: +40° C./75% RH

	Initial	1 Month	2 Months	3 Months	6 Months	9 Months	12 Months

Appearance¹	conforms	conforms	conforms	OOS*	OOS**	OOS**	OOS**
pH²	7.6	7.64	7.6	7.6	7.6	7.5	7.5
Assay	15.6	15.6	15.4	15.1	14.6	14.4	14.1
[mg/mL]
Purity [%]³	99.0	98.8	98.6	98.1	97.2	96.2	95.9
Impurities:					0.3 @ 01.9	0.3 @ 01.9	0.3 @ 01.9
[%]⁴						0.2 @ 020	0.2 @ 020
	n.d.	0.2% @ 0.23	0.2% @ 0.23	0.2% @ 0.23	0.4% @ 0.23	0.3% @ 0.23	0.3% @ 0.23
						0.3% @ 0.24	0.3% @ 024
						0.2% @ 0.29	0.2% @ 0.29
			0.2% @ 0.34	0.2% @ 0.34	0.2% @ 0.34	0.3% @ 0.33	0.3% @ 0.32
							0.2% @ 0.36
					0.2% @ 0.48	0.2% @ 0.47	0.2% @ 0.47
						0.2% @ 0.74	0.2% @ 0.74
				0.2% @ 0.83	0.3% @ 0.83	0.3% @ 0.82	0.4% @ 0.82
	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.89	0.4% @ 0.88	0.4% @ 0.88
	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91	0.4% @ 0.91
	0.2% @ 1.02	0.2% @ 1.02	0.2% @ 1.02	0.3% @ 1.02	0.4% @ 1.04	0.5% @ 1.02
				0.2% @ 1.10	0.4% @ 1.10	0.4% @ 1.10	0.5% @ 1.11

OOS*: Solution turned yellow
OOS**: Solution turned yellow (nonconformance confirmed)

Example 8: Stability Testing of Aileron Peptide 1

Aileron peptide 1 was performed on a pharmaceutical formulation formulated at a concentration of 15 mg/mL (20 mM sodium phosphate, 240 mM D-Trehalose, 330 ppm polysorbate 20, pH 7.5). 5 mL of this formulation was stored in a 10 mL clear serum vial (20 mm FluroTec®-coated stopper; 20 mm Flip-off™ seal). The formulation was stored at −15° C. and was tested at regular intervals. The results of this analysis are summarized in Table 12.

TABLE 12

Attribute	Test	Specification	Results

	Appearance	Upon thawing: Clear,	Conforms
		colorless,
		particulate-free solution
Identity	pH	7.5 ± 0.2	7.6
	Osmolality	220 to 400 mOsmol/Kg	327
	RP-HPLC (TFA)	Co-elutes with	Conforms
		ALRN-6924
		reference standard
Potency	qRP-HPLC (TFA)	15 ± 1.5 mg/mL	15.6
Purity	RP-HPLC (TFA)	Purity ≥ 95%	99.0%
		by area integration	RRT 0.88:
		no single	0.4%
		impurity > 3%¹	RRT 0.91:
			0.4%
			RRT 1.02:
			0.2%
Safety	Particulate matter	≥10 μm: ≤6,000	≥10 μm: 557
	USP <788>	particulates/vial	≥25 μm: 8
		≥25 μm: ≤600
		particulates/vial
	Endotoxin, USP	≤4.4 EU/mL²	<1.6 EU/mL
	<85>
	Container/Closure	No dye intrusion	Conforms
	Integrity
	Sterility; USP	No growth	No growth
	<71>
	(Membrane
	Filtration)

¹Impurities > 0.1% are listed based on their relative retention time (RRT) with respect to the Aileron peptide 1 peptide peak.
²Based on a maximum patient dose of 17 mg Aileron peptide 1 per Kg of patient weight.

Example 9: Stability Testing of Multiple Batches of Aileron Peptide 1 Under Varying Storage Conditions

Samples 1-7 of Aileron peptide 1 were formulated at a concentration of 15 mg/mL (20 mM sodium phosphate, 240 mM D-Trehalose, 330 ppm polysorbate 20, pH 7.5). These samples were stored under different storage conditions as described in Table 13. The formulations were tested for appearances and purity. The results are summarized in Tables 14-16 below.

TABLE 13

Sample Number	Sample Description

Sample
1	Aileron peptide 1 Drug product configuration
	Upright
	15 mg/mL
	12 month
	−20° C.(/−5° C.)
Sample 2	Aileron peptide 1 Drug product configuration
	Upright Storage Only/Return
	15 mg/mL
	12 month
	−20° C. (+/−5° C.)
Sample 3	Aileron peptide 1 Drug product configuration
	Inverted Storage Only/Return
	15 mg/mL
	12 month
	25° C./60% RH (+/−2° C./ +/−5% RH)
Sample 4	Aileron peptide 1 Drug product configuration
	Inverted
	15 mg/mL
	12 month
	25° C./60% RH (+/−2° C./ +/−5% RH)
Sample 5	Aileron peptide 1 Drug product configuration
	Inverted Storage Only/Return
	15 mg/mL
	12 month
	5° C. (+/−3° C.)
Sample 6	Aileron peptide 1 Drug product configuration
	Inverted
	15 mg/mL
	12 month
	5° C. (+/−3° C.)
Sample 7	Aileron peptide 1 Drug product configuration
	Inverted
	15 mg/mL
	12 month
	40° C./75% RH (+/−2° C./ +/−5% RH)

TABLE 14

Results of analysis of sample 1.

	Acceptance
Test	Criteria	Test Result(s)

Appearance	Upon thawing:	Clear, colorless, particulate-free
	Clear, colorless,	solution.
	particulate-free	Meets acceptance criteria.
	solution
pH	7.3 to 7.7	7.63
		Meets acceptance criteria.
RP_HPLC (TFA)	≥13.5 mg/mL;	Injection #1 = 15.58 mg/mL
for Aileron	≤16.5 mg/mL	Injection #	2 = 15.54 mg/mL
peptide-1		Mean = 15.6 mg/mL
Concentration		Meets acceptance criteria.
RP_HPLC (TFA)	≥95% for	Injection #1 = 98.6%
Purity by Area	ALRN-6924 peak	Injection #	2 = 98.6%
Integration		Mean = 98.6%
		Meets acceptance criteria.
RP_HPLC (TFA)	No single	Injection #	1 = 0.41%
Purity by Area	impurity > 3%	Injection #	2 = 0.42%
Integration for:		Mean = 0.41%
Largest Impurity		Meets acceptance criteria.
(RRT 0.91)
RP_HPLC (TFA)	Report	Injection #	1 = 0.07%
Purity by Area	Result ≥ 0.1%	Injection #	2 = 0.07%
Integration for:		Mean = 0.07%
Impurity
(RRT 0.87)
RP_HPLC (TFA)	Report	Injection #	1 = 0.39%
Purity by Area	Result ≥ 0.1%	Injection #	2 = 0.39%
Integration for:		Mean = 0.39%
Impurity
(RRT 0.88)
RP_HPLC (TFA)	Report	Injection #	1 = 0.11%
Purity by Area	Result ≥ 0.1%	Injection #	2 = 0.10%
Integration for:		Mean = 0.11%
Impurity
(RRT 0.94)
RP_HPLC (TFA)	Report	Injection #	1 = 0.16%
Purity by Area	Result ≥ 0.1%	Injection #	2 = 0.14%
Integration for:		Mean = 0.15%
Impurity
(RRT 1.02)
RP_HPLC (TFA)	Report	Injection #	1 = 0.09%
Purity by Area	Result ≥ 0.1%	Injection #	2 = 0.09%
Integration for:		Mean = 0.09%
Impurity
(RRT 1.04)
RP_HPLC (TFA)P	Report	Injection #	1 = 0.07%
urity by Area	Result ≥ 0.1%	Injection #	2 = 0.07%
Integration for:		Mean = 0.07%
Impurity
(RRT 1.10)
Total Impurities	Information	1.3%

TABLE 15

Results of analysis of sample 4.

	Acceptance
Test	Criteria	Test Result(s)

Appearance	Upon thawing:	Clear, colorless,
	Clear, colorless,	particulate-free
	particulate-free	solution.
	solution	Meets acceptance
		criteria.
pH	7.3 to 7.7	7.64
		Meets acceptance
		criteria.
RP_HPLC (TFA) for: Aileron	≥13.5 mg/mL;	Injection #1 = 14.98
peptide-1 TEConcentration	≤16.5 mg/mL	mg/mL
		Injection #
2 = 15.00
		mg/mL
		Mean = 15.0 mg/mL
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	≥95% for Aileron	Injection #	1 = 96.3%
Integration	peptide-1 peak	Injection #	2 = 96.3%
		Mean = 96.3%
		Meets acceptance.
		criteria
RP_HPLC (TFA) Purity by Area	No single	Injection #	1 = 0.47%
Integration for: Largest Impurity	impurity > 3%	Injection #	2 = 0.47%
(RRT 0.23)		Mean = 0.47%
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.11%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.11%
0.16)		Mean = 0.11%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.13%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.13%
0.19)		Mean = 0.13%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.07%
0.22)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.10%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.10%
0.24)		Mean = 0.10%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.06%
0.25A)		Mean = 0.06%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.06%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.05%
0.25B)		Mean = 0.06%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.15%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.11%
0.28)		Mean = 0.13%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.11%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.09%
0.29)		Mean = 0.10%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.06%
0.31)		Mean = 0.06%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.21%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.20%
0.32)		Mean = 0.20%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.14%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.13%
0.36)		Mean = 0.13%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.08%
0.39)		Mean = 0.08%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.13%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.14%
0.41)		Mean = 0.14%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.12%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.14%
0.47)		Mean = 0.13%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.35%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.37%
0.82)		Mean = 0.36%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.07%
0.87)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.37%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.35%
0.88)		Mean = 0.36%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.36%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.35%
0.91)		Mean = 0.36%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.21%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.22%
1.02)		Mean = 0.22%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.10%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.10%
1.04)		Mean = 0.10%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.08%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.09%
1.10)		Mean = 0.09%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Impurity (RRT	Result ≥ 0.1%	Injection #2 = 0.08%
1.11)		Mean = 0.07%
Total Impurities	Information	3.6%

TABLE 16

Results of analysis of sample 6.

	Acceptance
Test	Criteria	Test Result(s)

Appearance	Upon thawing:	Clear, colorless,
	Clear, colorless,	particulate-free
	particulate-free	solution.
	solution	Meets acceptance
		criteria.
pH	7.3 to 7.7	7.64
		Meets acceptance
		criteria.
RP_HPLC (TFA) for Aileron	≥13.5 mg/mL;	Injection #1 = 15.61
peptide-1 Concentration	≤16.5 mg/mL	mg/mL
		Injection #
2 = 15.62
		mg/mL
		Mean = 15.6 mg/mL
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	≥95% for Aileron	Injection #	1 = 98.6%
Integration	peptide-1 peak	Injection #	2 = 98.6%
		Mean = 98.6%
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	No single	Injection #	1 = 0.43%
Integration for: Largest Impurity	impurity > 3%	Injection #	2 = 0.41%
(RRT 0.91)		Mean = 0.42%
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.10%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.10%
(RRT 0.82)		Mean = 0.10%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.08%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.06%
(RRT 0.87)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.39%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.40%
(RRT 0.88)		Mean = 0.40%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.08%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.11%
(RRT 0.94)		Mean = 0.10%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.15%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.14%
(RRT 1.02)		Mean = 0.14%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.06%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.09%
(RRT 1.04)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Largest Impurity	Result ≥ 0.1%	Injection #2 = 0.08%
(RRT 1.10)		Mean = 0.08%
Total Impurities	Information	1.4%

TABLE 17

Results of analysis of sample 7.

	Acceptance
Test	Criteria	Test Result(s)

Appearance	Upon thawing:	Clear, yellow,
	Clear, colorless,	particulate-free
	particulate-free	solution.
	solution	Does not meet
		acceptance criteria.
pH	7.3 to 7.7	7.45
		Meets acceptance
		criteria.
RP_HPLC (TFA) for Aileron	≥13.5 mg/mL;	Injection #1 = 14.09
peptide-1 Concentration	≤16.5 mg/mL	mg/mL
		Injection #
2 = 14.05
		mg/mL
		Mean = 14.1 mg/mL
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	≥95% for Aileron	Injection #	1 = 94.3%
Integration	peptide-1 peak	Injection #	2 = 94.3%
		Mean = 94.3%
		Does not meet
		acceptance criteria.
RP_HPLC (TFA) Purity by Area	No single	Injection #	1 = 0.58%
Integration for: Largest Impurity	impurity ≥ 3%	Injection #	2 = 0.59%
(RRT 1.02)		Mean = 0.59%
		Meets acceptance
		criteria.
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.07%
(RRT 0.11)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.07%
(RRT 0.12)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.08%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.08%
(RRT 0.16)		Mean = 0.08%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.39%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.39%
(RRT 0.19A)		Mean = 0.39%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.12%
Integration for: Largest Impurity	Results ≥0.1%	Injection #2 = 0.12%
(RRT 0.19B)		Mean = 0.12%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.15%
Integration for: Largest Impurity	Results ≥0.1%	Injection #2 = 0.15%
(RRT 0.20)		Mean = 0.15%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.26%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.26%
(RRT 0.23)		Mean = 0.26%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.27%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.27%
(RRT 0.24)		Mean = 0.27%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.15%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.14%
(RRT 0.25)		Mean = 0.14%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.10%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.09%
(RRT 0.28)		Mean = 0.09%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.17%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.18%
(RRT 0.29)		Mean = 0.17%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.30%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.30%
(RRT 0.32)		Mean = 0.30%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.16%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.15%
(RRT 0.36)		Mean = 0.16%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.14%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.14%
(RRT 0.41)		Mean = 0.14%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.06%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.06%
(RRT 0.43)		Mean = 0.06%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.19%
Integration for: Largest Impurity	Results > 0.1%	Injection #2 = 0.18%
(RRT 0.47)		Mean = 0.18%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.23%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.23%
(RRT 0.74)		Mean = 0.23%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.35%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.36%
(RRT 0.82)		Mean = 0.35%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.09%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.09%
(RRT 0.83)		Mean = 0.09%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.06%
(RRT 0.87)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.41%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.41%
(RRT 0.88)		Mean = 0.41%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.43%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.43%
(RRT 0.91)		Mean = 0.43%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.06%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.06%
(RRT 0.96)		Mean = 0.06%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.07%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.08%
(RRT 1.04)		Mean = 0.07%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.12%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.12%
(RRT 0.96)		Mean = 0.12%
RP_HPLC (TFA) Purity by Area	Report	Injection #1 = 0.49%
Integration for: Largest Impurity	Results ≥ 0.1%	Injection #2 = 0.49%
(RRT 1.11)		Mean = 0.49%
Total Impurities	Information	5.6%

Example 10: Von Heijne (VH) Value Calculation

von Heijne values were calculated using a method adapted from Hessa et al., Recognition of transmembrane helices by the endoplasmic reticulum translocon, Nature: 433, 377-381 (2005). Briefly, each amino acid is assigned a fixed value, regardless of location along the polypeptide chain, according to the Table 18 below:

TABLE 18

von Heijne Score of various amino acids

	Amino Acid	von Heijne Score

	I	−0.6
	L	−0.5
	Nle	−0.5
	$	−0.5
	St	−0.5
	$e	−0.5
	$r8	−0.5
	$r5	−0.5
	$s8	−0.5
	$s5	−0.5
	$er8	−0.5
	F	−0.3
	V	−0.3
	Aib	−0.1
	M	−0.1
	C	−0.1
	Abu	−0.1
	Ac-	0
	-NH₂	0
	A	0.1
	a	0.1
	W	0.3
	T	0.5
	Y	0.6
	G	0.6
	S	0.8
	N	2
	H	2
	P	2.2
	Q	2.2
	E	2.5
	R	2.5
	K	2.5
	D	3.5

The von Heijne value (VH) for the polypeptide is then calculated as the sum total of values for all amino acids in the polypeptide. For example, a pentapeptide of the sequence Ac-AAAAA-NH₂(SEQ ID NO: 5) would have a VH score of 5*(0.1)=0.5.

Example 11: Reverse-Phase HPLC Retention Time Determination

Peptides were analyzed by reverse-phase HPLC on a 100×2.1 mm Phenomenex 2.6 micron, 100 Angstrom C18 column using the following mobile phase gradient at room temperature:


Time	Flow rate	% A	% B
(min)	(mL/min)	(0.1% TFA in water)	(0.1% TFA in acetonitrile)

0	0.6	80	20
20.0	0.6	20	80
20.1	0.6	5	95
21.0	0.6	5	95
21.1	0.6	80	20
21.2	0.6	5	95
21.5	0.6	5	95
21.8	0.6	80	20
23.5	0.6	80	20

In some embodiments, the retention time (RT) was then normalized to a 0-100 scale by the following equation: RT=[RT_raw (from above)*3.317-0.534]*3.3333. In some embodiments, the retention times were not normalizd.

Claims

1.-293. (canceled)

294. An aqueous pharmaceutical formulation in a unit dosage form comprising:

(i) a peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof, wherein the peptidomimetic macrocycle;

(ii) a buffering agent;

(iii) a stabilizing agent that is polysorbate 20;

(iv) a tonicity agent that is trehalose;

wherein the peptidomimetic macrocycle has a Formula I:

wherein

each D, Xaa₅, Xaa₆, Xaa₈, Xaa₉, and E is independently an amino acid;

each E is independently selected from Ala, D-Ala, Aib, Sar, and Ser;

[D]_vis Leu₁-Thr₂;

Xaa₃is Phe;

Xaa₇is Trp;

Xaa₁₀is Leu;

each R₁and R₂is independently alkyl;

L is a macrocycle-forming linker;

R₇is —H;

R₈is —H; and

w is an integer from 1-10.

295. The aqueous pharmaceutical formulation of claim 294, wherein the peptidomimetic macrocycle has a length value of from 14 to 20 amino acids.

296. The aqueous pharmaceutical formulation of claim 294, wherein the peptidomimetic macrocycle has a von Heijne value of from 2 to 9.

297. The aqueous pharmaceutical formulation of claim 294, wherein the peptidomimetic macrocycle has a percent alanine content of from 15% to 40%.

298. The aqueous pharmaceutical formulation of claim 294, wherein a first, second, third, fourth, fifth, or sixth C-terminal amino acid of the peptidomimetic macrocycle is hydrophobic.

299. The aqueous pharmaceutical formulation of claim 294, wherein the peptidomimetic macrocycle comprises an α-helix.

300. The aqueous pharmaceutical formulation of claim 294, wherein the pharmaceutically acceptable salt of the peptidomimetic macrocycle is a sodium, potassium, lithium, calcium, zinc, or magnesium salt.

301. The aqueous pharmaceutical formulation of claim 294, wherein total peptidomimetic degradation products formed in the aqueous pharmaceutical formulation is less than 1.0% when stored at a temperature of 40° C. for a period of one month.

302. The aqueous pharmaceutical formulation of claim 294, wherein an osmolarity of the aqueous pharmaceutical formulation is from about 250 to about 1000 milliosmoles per kilogram.

303. The aqueous pharmaceutical formulation of claim 294, further comprising glucose, fructose, galactose, sucrose, lactose, maltose, or a mixture thereof.

304. The aqueous pharmaceutical formulation of claim 294, wherein the aqueous pharmaceutical formulation has a pH from about 6.0 to about 8.0.

305. The aqueous pharmaceutical formulation of claim 294, wherein the aqueous pharmaceutical formulation has a pH from about 4.0 to about 9.0.

306. The aqueous pharmaceutical formulation of claim 294, wherein the peptidomimetic macrocycle has a molecular weight in the range of 1800-2000 D.

307. A method of making an aqueous pharmaceutical formulation comprising adding greater than 15 mg/mL of a peptidomimetic macrocycle or a pharmaceutically acceptable salt thereof to water or an aqueous solution, wherein the aqueous pharmaceutical formulation comprises less than 2% w/v of any micelle forming agent.

308. The method of claim 307, wherein the peptidomimetic macrocycle is capable of binding to the MDM2 and/or MDMX proteins.

309. The method of claim 307, comprising adding a sodium salt of the peptidomimetic macrocycle to water or an aqueous solution.

311. The method of claim 307, further comprising adjusting the pH of the solution comprising the buffering agent and the stabilizing agent during the addition of the peptidomimetic macrocycle.

312. The method of claim 307, further comprising filtration of the aqueous pharmaceutical formulation obtained after the addition of the peptidomimetic macrocycle to the aqueous solution.

313. The aqueous pharmaceutical formulation of claim 294, wherein the aqueous pharmaceutical formulation is suitable for administration to a subject without dilution.