US20180140685A1 - A new therapeutic use of the botulinum neurotoxin serotype a - Google Patents

A new therapeutic use of the botulinum neurotoxin serotype a Download PDF

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US20180140685A1
US20180140685A1 US15/568,668 US201615568668A US2018140685A1 US 20180140685 A1 US20180140685 A1 US 20180140685A1 US 201615568668 A US201615568668 A US 201615568668A US 2018140685 A1 US2018140685 A1 US 2018140685A1
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botulinum neurotoxin
neurotoxin serotype
bont
use according
serotype
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Sara Marinelli
Flaminia Pavone
Siro LUVISETTO
Valentina Vacca
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Consiglio Nazionale delle Richerche CNR
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A61K38/4893—Botulinum neurotoxin (3.4.24.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0085—Brain, e.g. brain implants; Spinal cord
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin

Definitions

  • the present invention relates to a new therapeutic use of the botulinum neurotoxin serotype A.
  • HC heavy chain
  • LC light chain
  • botulinum neurotoxin in its different serotypes (A-G), has been used in the clinical practice for several years for the most varied syndromes and pathologies: in the treatment of muscular pathologies, pain syndromes and for the treatment of symptoms of neurodegenerative diseases. Moreover, its use in cosmetics is already widespread.
  • BoNT neurotransmitters/neuromodulators
  • this neurotoxin acts by cutting various components of the protein complex designated as SNARE.
  • the latter present in all cells, is essential in the exocytosis process when the synaptic vesicles must be anchored to the synaptic membrane from which the neurotransmitters will be released. The block of the formation of the SNARE complex prevents exocytosis and thereby transmission is inhibited.
  • botulinum neurotoxins not only to block acetylcholine but also various other neurotransmitters, such as glutamate, GABA and neuropeptides, such as SP and CGRP.
  • the heavy chain of the botulinum neurotoxin is particularly important for the penetration of the same inside the axonal ends. After the binding of the heavy chain with the terminal axon proteins, the neurotoxin can enter the neurons by endocytosis. The binding of the heavy chain occurs with the SV2 protein receptor, whose expression is increased when the synapse is more active. The light chain is able to leave the endocytic vesicles and reach the cytoplasm. The neurotoxin light chain has protease activity.
  • the botulinum neurotoxin serotype A proteolytically degrades the SNAP-25 protein, a type of SNARE protein.
  • the protein SNAP-25 is required for the release of the neurotransmitters from the axon terminals.
  • the botulinum neurotoxin degrades the SNAREs by preventing the release of the neurotransmitters at the synapses.
  • BoNT botulinum neurotoxin
  • BoNT has raised interest in the treatment of pain syndromes.
  • BoNT/A botulinum neurotoxin serotype A
  • BoNT/A botulinum neurotoxin serotype A
  • BoNT/A has analgesic and anti-inflammatory properties due to its ability to block the release of pro-inflammatory factors, such as substance P and glutamate. It can also be retrogradely transported and act on glial cells (Schwann cells, astrocytes and microglia), as well as on neurons (Marinelli S, Vacca V, Ricordy R, Uggenti C, Tata A M, et al. (2012) The Analgesic Effect on Neuropathic Pain of Retrogradely Transported botulinum Neurotoxin A Involves Schwann Cells and Astrocytes. PLoS ONE 7(10): e47977).
  • the U.S. Pat. No. 7,964,199 describes a pharmaceutical composition comprising a botulinum neurotoxin selected from serotypes A, B, C, D, E, F or G or a mixture of two of the above botulinum neurotoxins, for use in the therapeutic treatment of a neurological disorder selected from dystonia, spasmodic torticollis, blepharospasm, spasticity, migraine, lumbosciatica, spine disorders, and hypersalivation.
  • a neurological disorder selected from dystonia, spasmodic torticollis, blepharospasm, spasticity, migraine, lumbosciatica, spine disorders, and hypersalivation.
  • the International patent application WO 2006/013357 describes a composition or medicament comprising the botulinum neurotoxin serotype A2 and a surfactant, for use in the treatment of ophthalmological disorders, movement disorders, ENT disorders, gastrointestinal disorders, urogenital disorders, dermatological disorders, pain, inflammatory disorders, secretory disorders, respiratory disorders, hypertrophic disorders, articular disorders, endocrine disorders, autoimmune diseases, proliferative diseases, traumatic injuries, and veterinary disorders.
  • the International patent application WO 2009/80313 describes the use of BoNT/A in the therapy for spasticity due to spinal and cerebrovascular trauma.
  • the use of the botulinum neurotoxin serotype A for the treatment of spasticity is also described in US patent application US 2010/0124559.
  • botulinum neurotoxins in particular of the botulinum neurotoxin serotype A, is the duration of action in patients (from 2 to 6 months) at very low concentrations, in the fM or pM range. Although the mechanisms of the persistence of the effect are not clear, however, the duration of the intracellular enzymatic activity of the botulinum neurotoxin serotype A in rat spinal cord neurons was shown to be at least 10 months. This feature gives the botulinum neurotoxin serotype A a considerable advantage compared to common drugs, which need to be administered continuously so that the therapeutic effect is maintained.
  • BoNT serotype A BoNT serotype A
  • Dysport® Xeomin®
  • CBTXA BoNT serotype B
  • Myobloc®/NeuroBloc® BoNT serotype B
  • the approval process is extremely complex and varies from one preparation to another and from one country to another.
  • CBTXA is marketed only in China and little information is available on this product.
  • Xeomin® was approved recently in Germany.
  • the only BoNT/B marketed preparation (Myobloc®)/NeuroBloc®) has just been approved for cervical dystonia (CD) and only in a few countries. Its use is limited to patients who have developed neutralising antibodies against BoNT/A preparations.
  • Botox® is the most widely accepted and widespread preparation in the world, followed by Dysport® which, however, has not yet been approved in the United States of America.
  • botulinum neurotoxin serotype A is effective in the therapeutic treatment of paralysis caused by spinal cord injury.
  • an object of the present invention is the botulinum neurotoxin serotype A for use as defined in appended claim 1 , i.e. the therapeutic treatment of paralysis caused by spinal cord injury.
  • the paralysis caused by spinal cord injury is paraplegia or tetraplegia.
  • compositions comprising a pharmaceutically effective amount of the botulinum neurotoxin serotype A and at least one pharmaceutically acceptable carrier, excipient or diluent, for use in the therapeutic treatment of paralysis caused by spinal cord injury, particularly paraplegia or tetraplegia.
  • the botulinum neurotoxin serotype A is selected from the group consisting of botulinum neurotoxin serotype A1 and botulinum neurotoxin serotype A2.
  • the therapeutic treatment comprises administering to a human patient a pharmaceutically effective amount of the botulinum neurotoxin serotype A.
  • a pharmaceutically effective amount of botulinum neurotoxin serotype A is in the range of 75 U-360 U.
  • botulinum neurotoxin vary depending on the particular preparation of the neurotoxin used. Although the two most widespread commercial products both contain serotype A (Botox® and Dysport®), one unit of Botox® is not equivalent to one unit of Dysport®. On the basis of available studies, it was established that the dose ratio of Botox®:Dysport® is in the range of 1:3 to 1:5. The dose ratio for Botox®:Xeomin® is estimated at 1:1.
  • neurotoxin dose indicated for each preparation refers exclusively to that specific preparation.
  • the dose of neurotoxin related to Botox® is often used as a reference value.
  • the pharmaceutically effective dose of BoNT/A is thus expressed as units (U) related to Botox®.
  • the botulinum neurotoxin serotype A may be administered as a single dose or as a cumulative dose, divided over a predetermined period of time so as to optimize the therapeutic effect.
  • the determination of the administration regime falls within the skills of a person of average skill in the art.
  • the botulinum neurotoxin serotype A may be administered in any suitable manner, but the preferred administration mode is by injection.
  • the botulinum neurotoxin serotype A is administered intrathecally, more preferably by injection into the vertebral area immediately caudal to the area affected by the spinal cord injury.
  • a murine model of spinal cord injury was used, which accurately mimics the tissue damage resulting from a direct mechanical trauma and allows for reproducing the features of various human diseases caused by spinal cord injury (“Spinal Cord Injury”, SCI), including the total absence of motor recovery.
  • botulinum neurotoxin serotype A represents an effective treatment for paralysis caused by spinal cord injury (SCI).
  • the astrocytes contribute to the inhibitory environment inside the injured spinal cord: subsequent proliferation and hypertrophy thereof occur around the injury site.
  • the reactive astrocytes form astroglial scarring that acts as a barrier to axon regeneration. According to opposed observations, scar formation by astrocytes is essential to provide protection to the neurons after a lesion of the central nervous system, so there must be a balanced reaction, possibly modulated pharmacologically, to promote axon regeneration.
  • SCI initiates biochemical cascades that lead to an increase in the extracellular concentration of glutamate, resulting in excitotoxic events mediated by the glutamate receptor. After its release, specific transport proteins rapidly remove the extracellular glutamate from the synaptic cleft. The removal of excess glutamate prevents accumulation under normal conditions. However, with SCI, the concentration of extracellular glutamate increases up to neurotoxic levels. Excitotoxicity refers to the ability of glutamate to destroy neurons due to a prolonged excitatory synaptic transmission.
  • apoptosis mainly involves non-neuronal cells such as oligodendrocytes (ODs). Apoptotic cells are greater in number and closer to the epicentre of the lesion. Apoptosis of ODs leads to chronic demyelination, thus causing anterograde neurodegeneration. Moreover, SCI induces decreased expression of several myelin proteins. Recovery of ODs and preservation of myelin are expected to have a big effect on the functional outcome after SCI.
  • ODs oligodendrocytes
  • BoNT/A can be an effective treatment of SCI-caused paralysis as: (i) it is able to protect neuronal cells from excitotoxicity after injury, thanks to its ability to block glutamate release; ii) it is able to reduce the reactive astrocytes in order to balance the astroglial scarring, given its ability to reduce inflammation and act directly on the astrocytes; iii) it is able to stimulate OD proliferation in order to replace the apoptotic ODs.
  • FIG. 1 shows the locomotor recovery after intrathecal injection of BoNT/A (A).
  • BoNT/A (15 pg/5 microL) was administered within 1 hour after surgery and the first behavioural observation was made 24 hours after (D1). While mice injected with saline solution never recovered, animals treated with BoNT/A showed a significant improvement in the paraplegia (p ⁇ 0.0001) just two days (D2) after SCI and reached a total motor recovery (BMS score 9) 30 days after spinal injury. Sensitivity recovery after intrathecal injection of BoNT/A (B).
  • FIG. 2 shows confocal images of tissue samples proximal (prox) and distal (dist) with respect to the lesion, collected 30 (D30—panel (A) and 60 (D60—panel B) days after injury.
  • Expression of GFAP revealed a large astrogliosis both proximal and distal to the lesion in mice treated with saline solution, while in mice treated with BoNT/A it was only detectable in the proximal area.
  • Expression of c1-SNAP25 highlighted the long-term action of BoNT/A at D30 and D60 both in the proximal and the distal site and co-localization with GFAP confirmed that astrocytes are a target of BoNT/A.
  • FIG. 3 shows confocal images of tissue samples proximal (prox) and distal (dist) with respect to the lesion, collected 30 (D30—panel (A) and 60 (D60—panel B) days after injury.
  • Expression of NeuN showed intact neurons in the area proximal to the lesion in mice treated with BoNT/A, while in mice treated with saline solution the intact cell bodies were not visible (data not shown).
  • Expression of c1-SNAP25 highlighted the long-term action of BoNT/A at D30 and D60 both in the proximal and the distal site and co-localization with NeuN confirmed that neurons are a target of BoNT/A.
  • FIG. 4 shows the fluorescence analysis of GFAP and OLIG-1 expression 30 days after spinal injury.
  • the graph shows a significant reduction (p ⁇ 0.0001) of GFAP expression both in the dorsal horn (DH) and the ventral horn (VH) proximal and distal to the site of the lesion in comparison with mice treated with saline solution.
  • the evaluation of the fluorescence of OLIG-1 shows a significant increase (p ⁇ 0.05) of OLIG-1 expression in the distal part of the marrow both in the DH and the VH in animals treated with BoNT/A.
  • FIG. 5 shows OLIG-1 and c1-caspase expression in mice treated with saline solution and with BoNT/A 30 days after SCI proximal (A) and distal (B) to the site of the lesion.
  • c1-caspase is an apoptosis marker that is particularly expressed in mice treated with saline solution and strongly reduced by administration of BoNT/A both at the distal and proximal level in SCI mice.
  • c1-caspase staining in oligodendrocytes is particularly evident in mice treated with saline solution, while SCI mice treated with BoNT/A showed intact oligodendrocytes with little c1-caspase.
  • FIG. 6 shows NeuN and c1-caspase expression in mice treated with saline solution and with BoNT/A 30 days after SCI proximal (A) and distal (B) to the site of the lesion.
  • NeuN is a neuron marker that allows us to observe the presence of intact neurons both in the proximal ( FIG. 6 a ), and the distal ( FIG. 6 b ) area in mice treated with BoNT/A, while in mice treated with saline solution the intact cell bodies are not detectable in either of the areas ( FIGS. 6 a and 6 b ).
  • the graph in FIG. 6 c shows a significant reduction (p ⁇ 0.0001) of c1-caspase expression both in the dorsal horn (DH) and the ventral horn (VH) proximal and distal to the site of the lesion in comparison with mice treated with saline solution.
  • mice Male mice (Charles River Labs, Como, Italy) with an initial weight of approximately 30-35 g were used. Upon their arrival at the laboratory (at least 2 weeks before the experiments), the mice were housed in standard transparent plastic cages, in groups of 4 per cage, under standard conditions for animals (free access to food and water, 12:12 light/dark cycle, at a room temperature of 23° C.). The experiments were carried out between 11:00 am and 1:00 pm. The animals were treated and handled according to the guidelines of the Committee for Research and Ethical Issues of the IASP (PAIN® 1983, 16, 109-110) and the Italian and European laws (DLGs n.26 of 04/03/2014, European Directive 2010/63/EU) on the protection of animals used for scientific research.
  • SURGERY in order to cause the spinal cord injury, the animals were profoundly anaesthetized with a 1:1 mixture of Rompun (Bayer 20 mg/ml; 0.5 ml/kg) and Zoletil (100 mg/ml; 0.5 ml/kg), the hair of the back was shaved, the back disinfected with betadine and an incision was made to expose the backbone.
  • the animals were mounted on a stereotactic apparatus with spinal adapters connected to an electronic cortical impactor designated as “PinPoint” (Stoelting), which allows with pinpoint accuracy for the application of a point force in the area to be injured, and maintained at 37° C. throughout the surgery.
  • the spinal cord was injured at the thoracic level 10 (T10) and no laminectomy was performed.
  • the bladder was emptied by manual abdominal pressure twice a day until restoration of bladder function, and the prophylactic antibiotic treatment (Baytril 2.5 mg/kg) was maintained for 1 week.
  • the animals were maintained at 37° with a heated plate, rehydrated with 1 ml of Ringer's lactate. To ensure feeding, wet food was placed in the cage.
  • TREATMENTS for the intrathecal injection, a volume of 5 ⁇ l of saline (0.9% NaCl) or of BoNT/A solution (0.937-15 pgtox/mouse) was injected in the mice at the spinal level L1/L2 using a microsyringe within 1 hour of the SCI.
  • the Basso Mouse Scale (BMS). The locomotor function of the hind legs was evaluated in the open field for all treatment groups. Mice were evaluated by two blinded evaluators.
  • the BMS score (Basso D M, Fisher L C, Anderson A J, Jakeman L B, McTigue D M, Popovich P G (2006) Basso Mouse Scale for locomotion detects differences in recovery after spinal cord injury in five common mouse strains. J Neurotrauma 23, 635-659) goes from 0-9, where 0 indicates complete paralysis and 9 indicates normal movement of the hind legs. The performance of the right paw and that of the left one were averaged to obtain the BMS score.
  • Tail Flick Test The mice were tested under the same conditions, i.e. at 7:00 a.m.-9:00 a.m. after 15 minutes of acclimatization. The environment in which the test was carried out was thoroughly washed between one animal and the other to eliminate any smell related to stress signals. A radiant heat source with a locator light (Ugo Basile) was placed on the tail and the latency to withdrawal was determined. A cutoff time of 10 seconds was used to prevent tissue damage. The latency to withdrawal or to a flick of the tail away from the heat source was recorded with a built-in timer, which showed the reaction time in 0.01 second increments. The tests were carried out three times with a 5 minute rest between sequences, and the average latency was recorded. All tests were carried out by researchers blinded to the treatment.
  • locator light Ugo Basile
  • mice treated with saline showed a total absence of thermal sensitivity after SCI. They always reached the latency threshold limit (10 sec) and they never recovered during the test period TF ( FIG. 1B ). On the other hand, the mice treated with BoNT/A began to restore the thermal threshold already two days after the injury and restored it completely at day 20 (p ⁇ 0.0001).
  • mice for each experimental group were sacrificed for immunohistochemical analysis and perfused with saline followed by 4% paraformaldehyde in phosphate buffered saline (PBS, pH 7.4).
  • the thoracic spinal cord (T1-T13) of the mice was collected and kept in immersion for 48 hours in 4% paraformaldehyde in phosphate buffered saline (PBS, pH 7.4) after cryoprotection with 30% (w/v) sucrose solution in PBS and maintained at ⁇ 80° C.
  • Cryostat sections of 40 ⁇ m were obtained. For the double IF staining, different sections were incubated for 48 hours at room temperature with primary antibodies (see Table 2) in 0.3% Triton.
  • the sections were then washed in PBS and incubated for 2 hours at room temperature with the secondary antibodies (see table).
  • the sections were washed again in PBS and incubated for 10 minutes with Bisbenzimide (Hoechst 33258, 1:1000, Jackson ImmunoResearch) to stain the nuclei. After washing in PBS, the sections were mounted on slides.
  • IF images at low magnification (10 ⁇ objective) and high magnification (63 ⁇ objective) from immunostained spinal cord sections were captured by confocal laser scanning microscopy using a TCS SP5 microscope (Leica Microsystems, Milan, Italy) connected to diagnostic instruments with a digital camera controlled by the LAS AF lite software from Leica Microsystems (free download available at www.leica-mycrosystems.com).
  • LC/BoNTA Receptor SNAP (Soluble NSF Attachment Protein) (SNARE) proteins in the cytosol leading to inhibition of exocytosis of neurotransmitter-carrying vesicles.
  • SNARE Soluble NSF Attachment Protein
  • LC/BoNTA was shown to be located on the neuronal cell membranes. Further studies showed that the localization of LC/BoNTA on the membranes was due to the high affinity binding with its substrate SNAP25 that is localized on the membranes.
  • the heavy chain is essential for entering the axon endings, by binding the SV2 receptor, and for allowing entry of BoNT/A in neurons through the endocytosis process.
  • the light chain, inside the cell has protease activity and degrades SNAP-25, which is required for the formation of the SNARE complex and the subsequent vesicular release of neurotransmitters at the synaptic level.
  • BoNTs particularly of serotype A
  • the duration of action 2-6 months, in patients, at concentrations in the fM or pM range.
  • the mechanisms of this persistence of the effects are not yet clear.
  • the duration of the intracellular enzymatic activity of BoNT/A in rat spinal cord neurons was shown to be at least 10 months (Whitemarsh R C, Tepp W H, Johnson E A, Pellett S. (2014) Persistence of botulinum neurotoxin a subtypes 1-5 in primary rat spinal cord cells. PLoS One. 9(2):e90252).
  • This feature gives BoNT/A an advantage compared to common drugs, which need to be administered continuously so that their therapeutic effects are maintained.
  • BoNT/A which cuts SNAP-25, is extremely promising as a therapeutic treatment for lesions caused by SCI.
  • c1-SNAP25 in the spinal cord of injured mice treated with BoNT/A or saline solution, to check if BoNT/A was still active 30 and 60 days after the i.th administration, which cells were suffering from poisoning by BoNT/A, and if BoNT/A reached sites distal to the injection spot.
  • c1-SNAP25 was detected only in the tissue of mice treated with BoNT/A and was totally absent from the spinal cord of mice treated with saline solution ( FIG. 2A , B); activated astrocytes and neurons ( FIG. 3A , B) were rich in c1-SNAP25 and BoNT/A decreased astrogliosis in the area distal to the lesion.
  • mice can be considered as the end of the subacute/intermediate phase and the beginning of the chronic phase.
  • this transition period features maturation/stabilization of the lesion, including continuous formation of a scar and development of cysts and/or syringomyelia (pathological tubular cavities in the organism), and is accompanied by alterations in neuronal circuits.
  • a somewhat delayed astrocytic response starts in the subacute phase (within the first 7 days), during which the astrocytes at the lesion periphery become hypertrophic and proliferative, which correlates with a dramatic increase in the expression of the astrocyte intermediate filament, GFAP.
  • mice treated with saline solution neurons proximal to the lesion epicentre collapse ( FIG. 6 a ) and neuronal bodies are practically absent from, or barely detectable in, the distal areas ( FIG. 6 b ).
  • treatment with BoNT/A allowed for the sparing of a few neurons from death ( FIG. 6 a ) in the vicinity of the impact area and the sparing of a large number thereof in the distal areas ( FIG. 6 b ).

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