US20090298787A1

US20090298787A1 - Dsrna as Insect Control Agent

Info

Publication number: US20090298787A1
Application number: US12/087,536
Authority: US
Inventors: Romaan Raemaekers; Laurent Kubler; Els Vanbleu
Original assignee: Devgen NV
Current assignee: Devgen NV
Priority date: 2006-01-12
Filing date: 2007-01-12
Publication date: 2009-12-03
Also published as: CA2633576A1; US9528123B2; EP2377939A2; WO2007080126A3; EP1971687A2; EP2348115A3; WO2007080126A2; JP5474355B2; US20140373197A1; BRPI0706227A2; EP2348115A2; BRPI0706227A8; EP2377939A3; WO2007080126A9; JP2009523017A

Abstract

The present invention relates to methods for controlling pest infestation using double standard RNA molecules. The invention provides methods for making transgenic plants that express the double stranded RNA molecules, as well as pesticidal agents and commodity products produced by the inventive plants.

Description

FIELD OF THE INVENTION

The present invention relates to the field of double-stranded RNA (dsRNA)-mediated gene silencing in insect species. More particularly, the present invention relates to genetic constructs designed for the expression of dsRNA corresponding to novel target genes. These constructs are particularly useful in RNAi-mediated plant pest control. The invention further relates to methods for controlling insects, methods for preventing insect infestation and methods for down-regulating gene expression in insects using RNAi. The invention also relates to transgenic plants resistant to insect infestation.

BACKGROUND TO THE INVENTION

The environment is replete with pests and numerous methods have attempted to control pests infestations of plants. Commercial crops are often the targets of insect attack. Substantial progress has been made in the last few decades towards developing more efficient methods and compositions for controlling insect infestation in plants.
Chemical pesticides have been very effective in eradicating pest infestation. However, there are several disadvantages to using chemical pesticidal agents. Not only are they potentially detrimental to the environment, but they are not selective and are harmful to various crops and non-target fauna. Chemical pesticides persist in the environment and generally are slow to be metabolized, if at all. They accumulate in the food chain, and particularly in the higher predator species where they can act as mutagens and/or carcinogens to cause irreversible and deleterious genetic modifications. There has thus been continued controversy in the use of chemical insecticides to combat crop pests. They can rapidly develop resistance against these insecticides because of repetitive usage of the same insecticide or of insecticides having the same mode of action, and because accumulation also results in the development of resistance to the agents in species higher up the evolutionary ladder.
Control of insect pests on agronomically important crops is important, particularly insect pests which damage plants belonging to the Solanaceae family, especially potato (Solanum tuberosum), but also tomato (Solanum lycopersicum), eggplant (Solanum melongena), capsicums (Solanum capsicum), and nightshade (for example, Solanum aculeastrum, S. bulbocastanum, S. cardiophyllum, S. douglasii, S. dulcamara, S. lanceolatum, S. robustum, and S. triquetrum), particularly the control of coleopteran pests.
Biological control using extract from neem seed has been shown to work against coleopteran pests of vegetables. Commercially available neem-based insecticides have azadirachtin as the primary active ingredient. These insecticides are applicable to a broad spectrum of insects. They act as insect growth regulator; azadirachtin prevents insects from molting by inhibiting production of an insect hormone, ecdysone.
Biological control using protein Cry3A from Bacillus thuringiensis varieties tenebrionis and san diego, and derived insecticidal proteins are alternatives to chemical control. The Bt toxin protein is effective in controlling Colorado potato beetle larvae either as formulations sprayed onto the foliage or expressed in the leaves of potatoes.
An alternative biological agent is dsRNA. Over the last few years, down-regulation of genes (also referred to as “gene silencing”) in multicellular organisms by means of RNA interference or “RNAi” has become a well-established technique.
RNA interference or “RNAi” is a process of sequence-specific down-regulation of gene expression (also referred to as “gene silencing” or “RNA-mediated gene silencing”) initiated by double-stranded RNA (dsRNA) that is complementary in sequence to a region of the target gene to be down-regulated (Fire, A. Trends Genet. Vol. 15, 358-363, 1999; Sharp, P. A. Genes Dev. Vol. 15, 485-490, 2001).
Over the last few years, down-regulation of target genes in multicellular organisms by means of RNA interference (RNAi) has become a well established technique. Reference may be made to International Applications WO 99/32619 (Carnegie Institution) and WO 00/01846 (by Applicant).
DsRNA gene silencing finds application in many different areas, such as for example dsRNA mediated gene silencing in clinical applications (WO2004/001013) and in plants. In plants, dsRNA constructs useful for gene silencing have also been designed to be cleaved and to be processed into short interfering RNAs (siRNAs).
RNAi has also been proposed as a means of protecting plants against plant parasitic nematodes, i.e. by expressing in the plant (e.g. in the entire plant, or in a part, tissue or cell of a plant) one or more nucleotide sequences that form a dsRNA fragment that corresponds to a target gene in the plant parasitic nematode that is essential for its growth, reproduction and/or survival. Reference may be made to the International Application WO 00/01846 (by Applicant) and U.S. Pat. No. 6,506,559 (based on WO 99/32619).
Although the technique of RNAi has been generally known in the art in plants, C. elegans and mammalian cells for some years, to date little is known about the use of RNAi to down-regulate gene expression in insects. Since the filing and publication of the WO 00/01846 and WO 99/32619 applications, only few other applications have been published that relate to the use of RNAi to protect plants against insects. These include the International Applications WO 01/37654 (DNA Plant Technologies), WO 2005/019408 (Bar Ilan University), WO 2005/049841 (CSIRO, Bayer Cropscience), WO 05/047300 (University of Utah Research foundation), and the US application 2003/00150017 (Mesa et al.).
The present invention provides target genes and constructs useful in the RNAi-mediated insect pest control, especially the control of insect plant pathogens. The present invention also provides methods for controlling insect pest infestation by repressing, delaying, or otherwise reducing target gene expression within a particular insect pest.

DESCRIPTION OF THE INVENTION

The present invention describes a novel non-compound, non-protein based approach for the control of insect crop pests. The active ingredient is a nucleic acid, a double-stranded RNA (dsRNA), which can be used as an insecticidal formulation. In another embodiment, the dsRNA can be expressed constitutively in the host plant, plant part, plant cell or seed to protect the plant against chewing insects especially coleopterans such as beetles. The sequence of the dsRNA corresponds to part or whole of an essential insect gene and causes downregulation of the insect target via RNA interference (RNAi). As a result of the downregulation of mRNA, the dsRNA prevents expression of the target insect protein and hence causes death, growth arrest or sterility of the insect.
The methods of the invention can find practical application in any area of technology where it is desirable to inhibit viability, growth, development or reproduction of the insect, or to decrease pathogenicity or infectivity of the insect. The methods of the invention further find practical application where it is desirable to specifically down-regulate expression of one or more target genes in an insect. Particularly useful practical applications include, but are not limited to, protecting plants against insect pest infestation.
In accordance with one embodiment the invention relates to a method for controlling insect growth on a cell or an organism, or for preventing insect infestation of a cell or an organism susceptible to insect infection, comprising contacting insects with a double-stranded RNA, wherein the double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of the nucleotide sequence of an insect target gene, whereby the double-stranded RNA is taken up by the insect and thereby controls growth or prevents infestation.
The present invention therefore provides isolated novel nucleotide sequences of insect target genes, said isolated nucleotide sequences comprising at least one nucleic acid sequence selected from the group comprising:
(i) sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof,
(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof, and
(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof,
or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 49 to 158, 275 to 472, 533 to 575, 621 to 767, 813 to 862, 908 to 1040, 1161 to 1571, 1730 to 2039, 2120 to 2338, 2384 to 2460, or a complement thereof,
said nucleic acid sequences being useful for preparing the double stranded RNAs of the invention for controlling insect growth.
“Controlling pests” as used in the present invention means killing pests, or preventing pests to develop, or to grow or preventing pests to infect or infest. Controlling pests as used herein also encompasses controlling pest progeny (development of eggs). Controlling pests as used herein also encompasses inhibiting viability, growth, development or reproduction of the pest, or to decrease pathogenicity or infectivity of the pest. The compounds and/or compositions described herein, may be used to keep an organism healthy and may be used curatively, preventively or systematically to control pests or to avoid pest growth or development or infection or infestation.
Particular pests envisaged in the present invention are plant pathogenic insect pests. “Controlling insects” as used herein thus also encompasses controlling insect progeny (such as development of eggs). Controlling insects as used herein also encompasses inhibiting viability, growth, development or reproduction of the insect, or decreasing pathogenicity or infectivity of the insect. In the present invention, controlling insects may inhibit a biological activity in a insect, resulting in one or more of the following attributes: reduction in feeding by the insect, reduction in viability of the insect, death of the insect, inhibition of differentiation and development of the insect, absence of or reduced capacity for sexual reproduction by the insect, muscle formation, juvenile hormone formation, juvenile hormone regulation, ion regulation and transport, maintenance of cell membrane potential, amino acid biosynthesis, amino acid degradation, sperm formation, pheromone synthesis, pheromone sensing, antennae formation, wing formation, leg formation, development and differentiation, egg formation, larval maturation, digestive enzyme formation, haemolymph synthesis, haemolymph maintenance, neurotransmission, cell division, energy metabolism, respiration, apoptosis, and any component of eukaryotic cells cytoskeletal structure, such as, for example, actins and tubulins. The compounds and/or compositions described herein, may be used to keep an organism healthy and may be used curatively, preventively or systematically to control a insect or to avoid insect growth or development or infection or infestation. Thus, the invention may allow previously susceptible organisms to develop resistance against infestation by the insect organism.
The expression “complementary to at least part of” as used herein means that the nucleotide sequence is fully complementary to the nucleotide sequence of the target over more than two nucleotides, for instance over at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or more contiguous nucleotides.
According to a further embodiment, the invention relates to a method for down-regulating expression of a target gene in an insect, comprising contacting said insect with a double-stranded RNA, wherein the double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of the nucleotide sequence of the insect target gene to be down-regulated, whereby the double-stranded RNA is taken up into the insect and thereby down-regulates expression of the insect target gene.
Whenever the term “a” is used within the context of “a target gene”, this means “at least one” target gene. The same applies for “a” target organism meaning “at least one” target organism, and “a” RNA molecule or host cell meaning “at least one” RNA molecule or host cell. This is also detailed further below.
According to one embodiment, the methods of the invention rely on uptake by the insect of double-stranded RNA present outside of the insect (e.g. by feeding) and does not require expression of double-stranded RNA within cells of the insect. In addition, the present invention also encompasses methods as described above wherein the insect is contacted with a composition comprising the double-stranded RNA.
The invention further provides a method for down-regulating expression of at least one target gene in a target organism (which is capable of ingesting a plant, plant part, plant cell or seeds) comprising feeding a plant, plant part, plant cell or seed to the target organism which plant, plant part, plant cell or seed expresses double-stranded RNA.
In a more preferred aspect, the invention provides a method for down-regulating expression of at least one target gene in a target organism (which is capable of ingesting a host cell, or extracts thereof) comprising feeding a hostplant, plant part, plant cell or seed to the target organism which hostplant, plant part, plant cellcell or seed expresses a double-stranded RNA molecule comprising a nucleotide sequence complementary to or representing the RNA equivalent of at least part of the nucleotide sequence of the at least one target gene, whereby the ingestion of the host cell, host plant, plant part, plant cell or seed by the target organism causes and/or leads to down-regulation of expression of the at least one target gene.
The invention provides for use of a plant, plant part, plant cell or seed as defined herein for down regulation of expression of an insect target gene. In more detailed terms, the invention provides for use of a host cell as defined herein and/or an RNA molecule comprising a nucleotide sequence that is the RNA complement of or that represents the RNA equivalent of at least part of the nucleotide sequence of a target gene from a target organism, as produced by transcription of a nucleic acid molecule in a plant, plant part, plant cell or seed, for instance in the manufacture of a commodity product, for down regulation of expression of a target gene. Suitable target genes and target organisms in respect of the invention are discussed below in further detail.
According to one embodiment, the methods of the invention rely on a GMO approach wherein the double-stranded RNA is expressed by a cell or an organism infested with or susceptible to infestation by insects. Preferably, said cell is a plant cell or said organism is a plant.
The present invention thus also relates to a method for producing a plant resistant to a plant pathogenic insect, comprising:
transforming a plant cell with a recombinant construct comprising at least one regulatory sequence operably linked to a sequence complementary to at least part of (a) a nucleotide sequence of a target insect gene selected from the group consisting of:

- (i) sequences which are at least 75% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof,
- (ii) sequences comprising at least 17 contiguous nucleotides of any of SEQ ID Nos 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159,160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof, and
- (iii) sequences comprising a sense strand comprising a nucleotide sequence of (i) and an antisense strand comprising the complement of said nucleotide sequence of (i), wherein the transcript encoded by said nucleotide sequence is capable of forming a double-stranded RNA,
or (b) a nucleotide sequence which is an insect orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID Nos 49 to 158, 275 to 472, 533 to 575, 621 to 767, 813 to 862, 908 to 1040, 1161 to 1571, 1730 to 2039, 2120 to 2338, 2384 to 2460, or the complement thereof;
- regenerating a plant from the transformed plant cell; and
- growing the transformed plant under conditions suitable for the expression of the recombinant construct, said grown transformed plant resistant to plant pathogenic insects compared to an untransformed plant.

The insect can be any insect, meaning any organism belonging to the Kingdom Animals, more specific to the Phylum Arthropoda, and to the Class Insecta or the Class Arachnida. The methods of the invention are applicable to all insects and that are susceptible to gene silencing by RNA interference and that are capable of internalising double-stranded RNA from their immediate environment. The invention is also applicable to the insect at any stage in its development. Because insects have a non-living exoskeleton, they cannot grow at a uniform rate and rather grow in stages by periodically shedding their exoskeleton. This process is referred to as moulting or ecdysis. The stages between moults are referred to as “instars” and these stages may be targeted according to the invention. Also, insect eggs or live young may also be targeted according to the present invention. All stages in the developmental cycle, which includes metamorphosis in the pterygotes, may be targeted according to the present invention. Thus, individual stages such as larvae, pupae, nymph etc stages of development may all be targeted.
In one embodiment of the invention, the insect may belong to the following orders: Acari, Araneae, Anoplura, Coleoptera, Collembola, Dermaptera, Dictyoptera, Diplura, Diptera, Embioptera, Ephemeroptera, Grylloblatodea, Hemiptera, Homoptera, Hymenoptera, Isoptera, Lepidoptera, Mallophaga, Mecoptera, Neuroptera, Odonata, Orthoptera, Phasmida, Plecoptera, Protura, Psocoptera, Siphonaptera, Siphunculata, Thysanura, Strepsiptera, Thysanoptera, Trichoptera, and Zoraptera.
In preferred, but non-limiting, embodiments and methods of the invention the insect is chosen from the group consisting of an insect which is a plant pest, such as but not limited to Nilaparvata spp. (e.g. N. lugens (brown planthopper)); Laodelphax spp. (e.g. L. striatellus (small brown planthopper)); Nephotettix spp. (e.g. N. virescens or N. cincticeps (green leafhopper), or N. nigropictus (rice leafhopper)); Sogatella spp. (e.g. S. furcifera (white-backed planthopper)); Blissus spp. (e.g. B. leucopterus leucopterus (chinch bug)); Scotinophora spp. (e.g. S. vermidulate (rice blackbug)); Acrostemum spp. (e.g. A. hilare (green stink bug)); Pamara spp. (e.g. P. guttata (rice skipper)); Chilo spp. (e.g. C. suppressalis (rice striped stem borer), C. auricilius (gold-fringed stem borer), or C. polychrysus (dark-headed stem borer)); Chilotraea spp. (e.g. C. polychrysa (rice stalk borer)); Sesamia spp. (e.g. S. inferens (pink rice borer)); Tryporyza spp. (e.g. T. innotata (white rice borer), or T. incertulas (yellow rice borer)); Cnaphalocrocis spp. (e.g. C. medinalis (rice leafroller)); Agromyza spp. (e.g. A. oryzae (leafminer), or A. parvicomis (corn blot leafminer)); Diatraea spp. (e.g. D. saccharalis (sugarcane borer), or D. grandiosella (southwestern corn borer)); Narnaga spp. (e.g. N. aenescens (green rice caterpillar)); Xanthodes spp. (e.g. X. transverse (green caterpillar)); Spodoptera spp. (e.g. S. frugiperda (fall armyworm), S. exigua (beet armyworm), S. littoralis (climbing cutworm) or S. praefica (western yellowstriped armyworm)); Mythimna spp. (e.g. Mythmna (Pseudaletia) seperata (armyworm)); Helicoverpa spp. (e.g. H. zea (corn earworm)); Colaspis spp. (e.g. C. brunnea (grape colaspis)); Lissorhoptrus spp. (e.g. L. oryzophilus (rice water weevil)); Echinocnemus spp. (e.g. E. squamos (rice plant weevil)); Diclodispa spp. (e.g. D. armigera (rice hispa)); Oulema spp. (e.g. O. oryzae (leaf beetle); Sitophilus spp. (e.g. S. oryzae (rice weevil)); Pachydiplosis spp. (e.g. P. oryzae (rice gall midge)); Hydrellia spp. (e.g. H. griseola (small rice leafminer), or H. sasakii (rice stem maggot)); Chlorops spp. (e.g. C. oryzae (stem maggot)); Diabrotica spp. (e.g. D. virgifera virgifera (western corn rootworm), D. barberi (northern corn rootworm), D. undecimpunctata howardi (southern corn robtworm), D. virgifera zeae (Mexican corn rootworm); D. balteata (banded cucumber beetle)); Ostrinia spp. (e.g. O. nubilalis (European corn borer)); Agrotis spp. (e.g. A. ipsilon (black cutworm)); Elasmopalpus spp. (e.g. E. lignosellus (lesser cornstalk borer)); Melanotus spp. (wireworms); Cyclocephala spp. (e.g. C. borealis (northern masked chafer), or C. immaculata (southern masked chafer)); Popillia spp. (e.g. P. japonica (Japanese beetle)); Chaetocnema spp. (e.g. C. pulicaria (corn flea beetle)); Sphenophorus spp. (e.g. S. maidis (maize billbug)); Rhopalosiphum spp. (e.g. R. maidis (corn leaf aphid)); Anuraphis spp. (e.g. A. maidiradicis (corn root aphid)); Melanoplus spp. (e.g. M. femurubrum (redlegged grasshopper) M. differentialis (differential grasshopper) or M. sanguinipes (migratory grasshopper)); Hylemya spp. (e.g. H. platura (seedcorn maggot)); Anaphothrips spp. (e.g. A. obscrurus (grass thrips)); Solenopsis spp. (e.g. S. milesta (thief ant)); or spp. (e.g. T. urticae (twospotted spider mite), T. cinnabarinus (carmine spider mite); Helicoverpa spp. (e.g. H. zea (cotton bollworm), or H. armigera (American bollworm)); Pectinophora spp. (e.g. P. gossypiella (pink bollworm)); Earias spp. (e.g. E. vittella (spotted bollworm)); Heliothis spp. (e.g. H. virescens (tobacco budworm)); Anthonomus spp. (e.g. A. grandis (boll weevil)); Pseudatomoscelis spp. (e.g. P. seriatus (cotton fleahopper)); Trialeurodes spp. (e.g. T. abutiloneus (banded-winged whitefly) T. vaporariorum (greenhouse whitefly)); Bemisia spp. (e.g. B. argentifolii (silverleaf whitefly)); Aphis spp. (e.g. A. gossypii (cotton aphid)); Lygus spp. (e.g. L. lineolaris (tarnished plant bug) or L. hesperus (western tarnished plant bug)); Euschistus spp. (e.g. E. conspersus (consperse stink bug)); Chlorochroa spp. (e.g. C. sayi (Say stinkbug)); Nezara spp. (e.g. N. viridula (southern green stinkbug)); Thrips spp. (e.g. T. tabaci (onion thrips)); Frankliniella spp. (e.g. F. fusca (tobacco thrips), or F. occidentalis (western flower thrips)); Leptinotarsa spp. (e.g. L. decemlineata (Colorado potato beetle), L. juncta (false potato beetle), or L. texana (Texan false potato beetle)); Lema spp. (e.g. L. trilineata (three-lined potato beetle)); Epitrix spp. (e.g. E. cucumeris (potato flea beetle), E. hirtipennis (flea beetle), or E. tuberis (tuber flea beetle)); Epicauta spp. (e.g. E. vittata (striped blister beetle)); Phaedon spp. (e.g. P. cochleariae (mustard leaf beetle)); Epilachna spp. (e.g. E. varivetis (mexican bean beetle)); Acheta spp. (e.g. A. domesticus (house cricket)); Empoasca spp. (e.g. E. fabae (potato leafhopper)); Myzus spp. (e.g. M. persicae (green peach aphid)); Paratrioza spp. (e.g. P. cockerelli (psyllid)); Conoderus spp. (e.g. C. falli (southern potato wireworm), or C. vespertinus (tobacco wireworm)); Phthorimaea spp. (e.g. P. operculella (potato tuberworm)); Macrosiphum spp. (e.g. M. euphorbiae (potato aphid)); Thyanta spp. (e.g. T. pallidovirens (redshouldered stinkbug)); Phthorimaea spp. (e.g. P. operculella (potato tuberworm)); Helicoverpa spp. (e.g. H. zea (tomato fruitworm); Keiferia spp. (e.g. K. lycopersicella (tomato pinworm)); Limonius spp. (wireworms); Manduca spp. (e.g. M. sexta (tobacco hornworm), or M. quinquemaculata (tomato hornworm)); Liriomyza spp. (e.g. L. sativae, L. trifolli or L. huidobrensis (leafminer)); Drosophilla spp. (e.g. D. melanogaster, D. yakuba, D. pseudoobscura or D. simulans); Carabus spp. (e.g. C. granulatus); Chironomus spp. (e.g. C. tentanus); Ctenocephalides spp. (e.g. C. felis (cat flea)); Diaprepes spp. (e.g. D. abbreviatus (root weevil)); Ips spp. (e.g. I. pini (pine engraver)); Tribolium spp. (e.g. T. castaneum (red floor beetle)); Glossina spp. (e.g. G. morsitans (tsetse fly)); Anopheles spp. (e.g. A. gambiae (malaria mosquito)); Helicoverpa spp. (e.g. H. armigera (African Bollworm)); Acyrthosiphon spp. (e.g. A. pisum (pea aphid)); Apis spp. (e.g. A. melifera (honey bee)); Homalodisca spp. (e.g. H. coagulate (glassy-winged sharpshooter)); Aedes spp. (e.g. Ae. aegypti (yellow fever mosquito)); Bombyx spp. (e.g. B. mori (silkworm)); Locusta spp. (e.g. L. migratoria (migratory locust)); Boophilus spp. (e.g. B. microplus (cattle tick)); Acanthoscurria spp. (e.g. A. gomesiana (red-haired chololate bird eater)); Diploptera spp. (e.g. D. punctata (pacific beetle cockroach)); Heliconius spp. (e.g. H. erato (red passion flower butterfly) or H. melpomene (postman butterfly)); Curculio spp. (e.g. C. glandium (acorn weevil)); Plutella spp. (e.g. P. xylostella (diamondback moth)); Amblyomma spp. (e.g. A. variegatum (cattle tick)); Anteraea spp. (e.g. A. yamamai (silkmoth)); and Armigeres spp. (e.g. A. subalbatus);
Preferred plant pathogenic insects according to the invention are plant pest are selected from the group consisting of Leptinotarsa spp. (e.g. L. decemlineata (Colorado potato beetle), L. juncta (false potato beetle), or L. texana (Texan false potato beetle)); Nilaparvata spp. (e.g. N. lugens (brown planthopper)); Laodelphax spp. (e.g. L. striatellus (small brown planthopper)); Nephotettix spp. (e.g. N. virescens or N. cincticeps (green leafhopper), or N. nigropictus (rice leafhopper)); Sogatella spp. (e.g. S. furcifera (white-backed planthopper)); Chilo spp. (e.g. C. suppressais (rice striped stem borer), C. auricilius (gold-fringed stem borer), or C. polychrysus (dark-headed stem borer)); Sesamia spp. (e.g. S. inferens (pink rice borer)); Tryporyza spp. (e.g. T. innotata (white rice borer), or T. incertulas (yellow rice borer)); Anthonomus spp. (e.g. A. grandis (boll weevil)); Phaedon spp. (e.g. P. cochleariae (mustard leaf beetle)); Epilachna spp. (e.g. E. varivetis (mexican bean beetle)); Tribolium spp. (e.g. T. castaneum (red floor beetle)); Diabrotica spp. (e.g. D. virgifera virgifera (western corn rootworm), D. barberi (northern corn rootworm), D. undecimpunctata howardi (southern corn rootworm), D. virgifera zeae (Mexican corn rootworm); Ostrinia spp. (e.g. O. nubilalis (European corn borer)); Anaphothrips spp. (e.g. A. obscrurus (grass thrips)); Pectinophora spp. (e.g. P. gossypiella (pink bollworm)); Heliothis spp. (e.g. H. virescens (tobacco budworm)); Trialeurodes spp. (e.g. T. abutiloneus (banded-winged whitefly) T. vaporariorum (greenhouse whitefly)); Bemisia spp. (e.g. B. argentifolii (silverleaf whitefly)); Aphis spp. (e.g. A. gossypii (cotton aphid)); Lygus spp. (e.g. L. lineolaris (tarnished plant bug) or L. hesperus (western tarnished plant bug)); Euschistus spp. (e.g. E. conspersus (consperse stink bug)); Chlorochroa spp. (e.g. C. sayi (Say stinkbug)); Nezara spp. (e.g. N. viridula (southern green stinkbug)); Thrips spp. (e.g. T. tabaci (onion thrips)); Frankliniella spp. (e.g. F. fusca (tobacco thrips), or F. occidentalis (western flower thrips)); Acheta spp. (e.g. A. domesticus (house cricket)); Myzus spp. (e.g. M. persicae (green peach aphid)); Macrosiphum spp. (e.g. M. euphorbiae (potato aphid)); Blissus spp. (e.g. B. leucopterus leucopterus (chinch bug)); Acrostemum spp. (e.g. A. hilare (green stink bug)); Chilotraea spp. (e.g. C. polychrysa (rice stalk borer)); Lissorhoptrus spp. (e.g. L. oryzophilus (rice water weevil)); Rhopalosiphum spp. (e.g. R. maidis (corn leaf aphid)); and Anuraphis spp. (e.g. A. maidiradicis (corn root aphid)).
According to a more specific embodiment, the methods of the invention are applicable for Leptinotarsa species. Leptinotarsa belong to the family of Chrysomelidae or leaf beatles. Chrysomelid beetles such as Flea Beetles and Corn Rootworms and Curculionids such as Alfalfa Weevils are particularly important pests. Flea Beetles include a large number of small leaf feeding beetles that feed on the leaves of a number of grasses, cereals and herbs. Flea Beetles include a large number of genera (e.g., Attica, Apphthona, Argopistes, Disonycha, Epitrix, Longitarsus, Prodagricomela, Systena, and Phyllotreta). The Flea Beetle, Phyllotreta cruciferae, also known as the Rape Flea Beetle, is a particularly important pest. Corn rootworms include species found in the genus Diabrotica (e.g., D. undecimpunctata undecimpunctata, D. undecimpunctata howardii, D. longicornis, D. virgifera and D. balteata). Corn rootworms cause extensive damage to corn and curcubits. The Western Spotted Cucumber Beetle, D. undecimpunctata undecimpunctata, is a pest of curcubits in the western U.S. Alfalfa weevils (also known as clover weevils) belong to the genus, Hypera (H. postica, H. brunneipennis, H. nigrirostris, H. punctata and H. meles), and are considered an important pest of legumes. The Egyptian alfalfa weevil, H. brunneipennis, is an important pest of alfalfa in the western U.S.
There are more than 30 Leptinotarsa species. The present invention thus encompasses methods for controlling Leptinotarsa species, more specific methods for killing insects, or preventing Leptinotarsa insects to develop or to grow, or preventing insects to infect or infest. Specific Leptinotarsa species to control according to the invention include Colorado Potato Beetle (Leptinotarsa decemlineata (Say) and False Potato Beetle (Leptinotarsa juncta (Say).
CPB is a (serious) pest on our domestic potato (Solanum tuberosum), other cultivated and wild tuber bearing and non-tuber bearing potato species (e.g. S. demissum, S. phureja a.o.) and other Solanaceous (nightshades) plant species including:
(a) the crop species tomato (several Lycopersicon species), eggplant (Solanum melongena), peppers (several Capsicum species), tobacco (several Nicotiana species including ornamentals) and ground cherry (Physalis species);
(b) the weed/herb species, horse nettle (S. carolinense), common nightshade (S. dulcamara), belladonna (Atropa species), thorn apple (datura species), henbane (Hyoscyamus species) and buffalo burr (S. rostratum).
FPB is primarily found on horse nettle, but also occurs on common nightshade, ground cherry, and husk tomato (Physalis species).
The term “insect” encompasses insects of all types and at all stages of development, including egg, larval or nymphal, pupal and adult stages.
The present invention extends to methods as described herein, wherein the insect is Leptinotarsa decemlineata (Colorado potato beetle) and the plant is potato, eggplant, tomato, pepper, tobacco, ground cherry or rice, corn or cotton.
The present invention extends to methods as described herein, wherein the insect is Phaedon cochleariae (mustard leaf beetle) and the plant is mustard, chinese cabbage, turnip greens, collard greens or bok choy.
The present invention extends to methods as described herein, wherein the insect is Epilachna varivetis (Mexican bean beetle) and the plants are beans, field beans, garden beans, snap beans, lima beans, mung beans, string beans, black-eyed beans, velvet beans, soybeans, cowpeas, pigeon peas, clover or alfalfa.
The present invention extends to methods as described herein, wherein the insect is Anthonomus grandis (cotton boll weevil) and the plant is cotton.
The present invention extends to methods as described herein, wherein the insect is Tribolium castaneum (red flour beetle) and the plant is in the form of stored grain products such as flour, cereals, meal, crackers, beans, spices, pasta, cake mix, dried pet food, dried flowers, chocolate, nuts, seeds, and even dried museum specimens.
The present invention extends to methods as described herein, wherein the insect is Myzus persicae (green peach aphid) and the plant is a tree such as Prunus, particularly peach, apricot and plum; a vegetable crop of the families Solanaceae, Chenopodiaceae, Compositae, Cruciferae, and Cucurbitaceae, including but not limited to, artichoke, asparagus, bean, beets, broccoli, Brussels sprouts, cabbage, carrot, cauliflower, cantaloupe, celery, corn, cucumber, fennel, kale, kohlrabi, turnip, eggplant, lettuce, mustard, okra, parsley, parsnip, pea, pepper, potato, radish, spinach, squash, tomato, turnip, watercress, and watermelon; a field crops such as, but not limited to, tobacco, sugar beet, and sunflower; a flower crop or other ornamental plant.
The present invention extends to methods as described herein, wherein the insect is Nilaparvata lugens and the plant is a rice plant.
The present invention extends to methods as described herein, wherein the insect is Chilo suppressalis (rice striped stem borer) and the plant is a rice plant, bareley, sorghum, maize, wheat or a grass.
The present invention extends to methods as described herein, wherein the insect is Plutella xylostella (Diamondback moth) and the plant is a Brassica species such as, but not limited to cabbage, chinese cabbage, Brussels sprouts, kale, rapeseed, broccoli, cauliflower, turnip, mustard or radish.
The present invention extends to methods as described herein, wherein the insect is Acheta domesticus (house cricket) and the plant is any plant as described herein or any organic matter.
In terms of “susceptible organisms”, which benefit from the present invention, any organism which is susceptible to pest infestation is included. Preferably plants may benefit from the present invention by protection from infestation by plant pest organisms.
In a preferred embodiment the susceptible organism is a plant and the pest is a plant pathogenic insect. In this embodiment the insect is contacted with the RNA molecule by expressing the dsRNA molecule in a plant, plant part, plant cell or plant seed that is infested with or susceptible to infestation with the plant pathogenic pest.
In this context the term “plant” encompasses any plant material that it is desired to treat to prevent or reduce insect growth and/or insect infestation. This includes, inter alia, whole plants, seedlings, propagation or reproductive material such as seeds, cuttings, grafts, explants, etc. and also plant cell and tissue cultures. The plant material should express, or have the capability to express, the RNA molecule comprising at least one nucleotide sequence that is the RNA complement of or that represents the RNA equivalent of at least part of the nucleotide sequence of the sense strand of at least one target gene of the pest organism, such that the RNA molecule is taken up by a pest upon plant-pest interaction, said RNA molecule being capable of inhibiting the target gene or down-regulating expression of the target gene by RNA interference.
The target gene may be any of the target genes herein described, for instance a target gene that is essential for the viability, growth, development or reproduction of the pest. The present invention relates to any gene of interest in the insect (which may be referred to herein as the “target gene”) that can be down-regulated.
The terms “down-regulation of gene expression” and “inhibition of gene expression” are used interchangeably and refer to a measurable or observable reduction in gene expression or a complete abolition of detectable gene expression, at the level of protein product and/or mRNA product from the target gene. Preferably the down-regulation does not substantially directly inhibit the expression of other genes of the insect. The down-regulation effect of the dsRNA on gene expression may be calculated as being at least 30%, 40%, 50%, 60%, preferably 70%, 80% or even more preferably 90% or 95% when compared with normal gene expression. Depending on the nature of the target gene, down-regulation or inhibition of gene expression in cells of an insect can be confirmed by phenotypic analysis of the cell or the whole insect or by measurement of mRNA or protein expression using molecular techniques such as RNA solution hybridization, PCR, nuclease protection, Northern hybridization, reverse transcription, gene expression monitoring with a microarray, antibody binding, enzyme-linked immunosorbent assay (ELISA), Western blotting, radioimmunoassay (RIA), other immunoassays, or fluorescence-activated cell analysis (FACS).
The “target gene” may be essentially any gene that is desirable to be inhibited because it interferes with growth or pathogenicity or infectivity of the insect. For instance, if the method of the invention is to be used to prevent insect growth and/or infestation then it is preferred to select a target gene which is essential for viability, growth, development or reproduction of the insect, or any gene that is involved with pathogenicity or infectivity of the insect, such that specific inhibition of the target gene leads to a lethal phenotype or decreases or stops insect infestation.
According to one non-limiting embodiment, the target gene is such that when its expression is down-regulated or inhibited using the method of the invention, the insect is killed, or the reproduction or growth of the insect is stopped or retarded. This type of target genes is considered to be essential for the viability of the insect and is referred to as essential genes. Therefore, the present invention encompasses a method as described herein, wherein the target gene is an essential gene.
According to a further non-limiting embodiment, the target gene is such that when it is down-regulated using the method of the invention, the infestation or infection by the insect, the damage caused by the insect, and/or the ability of the insect to infest or infect host organisms and/or cause such damage, is reduced. The terms “infest” and “infect” or “infestation” and “infection” are generally used interchangeably throughout. This type of target genes is considered to be involved in the pathogenicity or infectivity of the insect. Therefore, the present invention extends to methods as described herein, wherein the target gene is involved in the pathogenicity or infectivity of the insect. The advantage of choosing the latter type of target gene is that the insect is blocked to infect further plants or plant parts and is inhibited to form further generations.
According to one embodiment, target genes are conserved genes or insect-specific genes.
In addition, any suitable double-stranded RNA fragment capable of directing RNAi or RNA-mediated gene silencing or inhibition of an insect target gene may be used in the methods of the invention.
In another embodiment, a gene is selected that is essentially involved in the growth, development, and reproduction of a pest, (such as an insect). Exemplary genes include but are not limited to the structural subunits of ribosomal proteins and a beta-coatamer gene, such as the CHD3 gene. Ribosomal proteins such as S4 (RpS4) and S9(RpS9) are structural constituents of the ribosome involved in protein biosynthesis and which are components of the cytosolic small ribosomal subunit, the ribosomal proteins such as L9 and L19 are structural constituent of ribosome involved in protein biosynthesis which is localised to the ribosome. The beta coatamer gene in C. elegans encodes a protein which is a subunit of a multimeric complex that forms a membrane vesicle coat. Similar sequences have been found in diverse organisms such as Arabidopsis thaliana, Drosophila melanogaster, and Saccharomyces cerevisiae. Related sequences are found in diverse organisms such as Leptinotarsa decemlineata, Phaedon cochlearae, Epilachna varivestis, Anthonomus grandis, Trbolium castaneum, Myzus persicae, Nilaparvata lugens, Chilo suppressalis, Plutella xylostella and Acheta domesticus.
Other target genes for use in the present invention may include, for example, those that play important roles in viability, growth, development, reproduction, and infectivity. These target genes include, for example, house keeping genes, transcription factors, and pest specific genes or lethal knockout mutations in Caenorhabditis or Drosophila. The target genes for use in the present invention may also be those that are from other organisms, e.g., from insects or arachnidae (e.g. Leptinotarsa spp., Phaedon spp., Epilachna spp., Anthonomus spp., Tribolium spp., Myzus spp., Nilaparvata spp., Chilo spp., Plutella spp., or Acheta spp.).
Preferred target genes include those specified in Table 1A and orthologous genes from other target organisms, such as from other pest organisms.
In the methods of the present invention, dsRNA is used to inhibit growth or to interfere with the pathogenicity or infectivity of the insect.
The invention thus relates to isolated double-stranded RNA comprising annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of a target nucleotide sequence of a target gene of an insect. The target gene may be any of the target genes described herein, or a part thereof that exerts the same function.
According to one embodiment of the present invention, an isolated double-stranded RNA is provided comprising annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of a nucleotide sequence of an insect target gene, wherein said target gene comprises a sequence which is selected from the group comprising:

- (i) sequences which are at least 75% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof, and
- (ii) sequences comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof,
  or wherein said insect target gene is an insect orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 49 to 158, 275 to 472, 533 to 575, 621 to 767, 813 to 862, 908 to 1040, 1161 to 1571, 1730 to 2039, 2120 to 2338, 2384 to 2460, or the complement thereof.

Depending on the assay used to measure gene silencing, the growth inhibition can be quantified as being greater than about 5%, 10%, more preferably about 20%, 25%, 33%, 50%, 60%, 75%, 80%, most preferably about 90%, 95%, or about 99% as compared to a pest organism that has been treated with control dsRNA.
According to another embodiment of the present invention, an isolated double-stranded RNA is provided, wherein at least one of said annealed complementary strands comprises the RNA equivalent of at least one of the nucleotide sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or wherein at least one of said annealed complementary strands comprises the RNA equivalent of a fragment of at least 17 basepairs in length thereof, preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 basepairs in length thereof.
If the method of the invention is used for specifically controlling growth or infestation of a specific insect in or on a host cell or host organism, it is preferred that the double-stranded RNA does not share any significant homology with any host gene, or at least not with any essential gene of the host. In this context, it is preferred that the double-stranded RNA shows less than 30%, more preferably less that 20%, more preferably less than 10%, and even more preferably less than 5% nucleic acid sequence identity with any gene of the host cell. % sequence identity should be calculated across the full length of the double-stranded RNA region. If genomic sequence data is available for the host organism one may cross-check sequence identity with the double-stranded RNA using standard bioinformatics tools. In one embodiment, there is no sequence identity between the dsRNA and a host sequences over 21 contiguous nucleotides, meaning that in this context, it is preferred that 21 contiguous base pairs of the dsRNA do not occur in the genome of the host organism. In another embodiment, there is less than about 10% or less than about 12.5% sequence identity over 24 contiguous nucleotides of the dsRNA with any nucleotide sequence from a host species.
The double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which corresponds to a target nucleotide sequence of the target gene to be down-regulated. The other strand of the double-stranded RNA is able to base-pair with the first strand.
The expression “target region” or “target nucleotide sequence” of the target insect gene may be any suitable region or nucleotide sequence of the gene. The target region should comprise at least 17, at least 18 or at least 19 consecutive nucleotides of the target gene, more preferably at least 20 or at least 21 nucleotide and still more preferably at least 22, 23 or 24 nucleotides of the target gene.
It is preferred that (at least part of) the double-stranded RNA will share 100% sequence identity with the target region of the insect target gene. However, it will be appreciated that 100% sequence identity over the whole length of the double stranded region is not essential for functional RNA inhibition. RNA sequences with insertions, deletions, and single point mutations relative to the target sequence have also been found to be effective for RNA inhibition. The terms “corresponding to” or “complementary to” are used herein interchangeable, and when these terms are used to refer to sequence correspondence between the double-stranded RNA and the target region of the target gene, they are to be interpreted accordingly, i.e. as not absolutely requiring 100% sequence identity. However, the % sequence identity between the double-stranded RNA and the target region will generally be at least 80% or 85% identical, preferably at least 90%, 95%, 96%, or more preferably at least 97%, 98% and still more preferably at least 99%. Two nucleic acid strands are “substantially complementary” when at least 85% of their bases pair.
The term “complementary” as used herein relates to both DNA-DNA complementarity as to DNA-RNA complementarity. In analogy herewith, the term “RNA equivalent” substantially means that in the DNA sequence(s), the base “T” may be replaced by the corresponding base “U” normally present in ribonucleic acids.
Although the dsRNA contains a sequence which corresponds to the target region of the target gene it is not absolutely essential for the whole of the dsRNA to correspond to the sequence of the target region. For example, the dsRNA may contain short non-target regions flanking the target-specific sequence, provided that such sequences do not affect performance of the dsRNA in RNA inhibition to a material extent.
The dsRNA may contain one or more substitute bases in order to optimise performance in RNAi. It will be apparent to the skilled reader how to vary each of the bases of the dsRNA in turn and test the activity of the resulting dsRNAs (e.g. in a suitable in vitro test system) in order to optimise the performance of a given dsRNA.
The dsRNA may further contain DNA bases, non-natural bases or non-natural backbone linkages or modifications of the sugar-phosphate backbone, for example to enhance stability during storage or enhance resistance to degradation by nucleases.
It has been previously reported that the formation of short interfering RNAs (siRNAs) of about 21 bp is desirable for effective gene silencing. However, in applications of applicant it has been shown that the minimum length of dsRNA preferably is at least about 80-100 bp in order to be efficiently taken up by certain pest organisms. There are indications that in invertebrates such as the free living nematode C. elegans or the plant parasitic nematode Meloidogyne incognita, these longer fragments are more effective in gene silencing, possibly due to a more efficient uptake of these long dsRNA by the invertebrate.
It has also recently been suggested that synthetic RNA duplexes consisting of either 27-mer blunt or short hairpin (sh) RNAs with 29 bp stems and 2-nt 3′ overhangs are more potent inducers of RNA interference than conventional 21-mer siRNAs. Thus, molecules based upon the targets identified above and being either 27-mer blunt or short hairpin (sh) RNA's with 29-bp stems and 2-nt 3′overhangs are also included within the scope of the invention.
Therefore, in one embodiment, the double-stranded RNA fragment (or region) will itself preferably be at least 17 bp in length, preferably 18 or 19 bp in length, more preferably at least 20 bp, more preferably at least 21 bp, or at least 22 bp, or at least 23 bp, or at least 24 bp, 25 bp, 26 bp or at least 27 bp in length. The expressions “double-stranded RNA fragment” or “double-stranded RNA region” refer to a small entity of the double-stranded RNA corresponding with (part of) the target gene.
Generally, the double stranded RNA is preferably between about 17-1500 bp, even more preferably between about 80-1000 bp and most preferably between about 17-27 bp or between about 80-250 bp; such as double stranded RNA regions of about 17 bp, 18 bp, 19 bp, 20 bp, 21 bp, 22 bp, 23 bp, 24 bp, 25 bp, 27 bp, 50 bp, 80 bp, 100 bp, 150 bp, 200 bp, 250 bp, 300 bp, 350 bp, 400 bp, 450 bp, 500 bp, 550 bp, 600 bp, 650 bp, 700 bp, 900 bp, 100 bp, 1100 bp, 1200 bp, 1300 bp, 1400 bp or 1500 bp.
The upper limit on the length of the double-stranded RNA may be dependent on i) the requirement for the dsRNA to be taken up by the insect and ii) the requirement for the dsRNA to be processed within the cell into fragments that direct RNAi. The chosen length may also be influenced by the method of synthesis of the RNA and the mode of delivery of the RNA to the cell. Preferably the double-stranded RNA to be used in the methods of the invention will be less than 10,000 bp in length, more preferably 1000 bp or less, more preferably 500 bp or less, more preferably 300 bp or less, more preferably 100 bp or less. For any given target gene and insect, the optimum length of the dsRNA for effective inhibition may be determined by experiment.
The double-stranded RNA may be fully or partially double-stranded. Partially double-stranded RNAs may include short single-stranded overhangs at one or both ends of the double-stranded portion, provided that the RNA is still capable of being taken up by insects and directing RNAi. The double-stranded RNA may also contain internal non-complementary regions.
The methods of the invention encompass the simultaneous or sequential provision of two or more different double-stranded RNAs or RNA constructs to the same insect, so as to achieve down-regulation or inhibition of multiple target genes or to achieve a more potent inhibition of a single target gene.
Alternatively, multiple targets are hit by the provision of one double-stranded RNA that hits multiple target sequences, and a single target is more efficiently inhibited by the presence of more than one copy of the double stranded RNA fragment corresponding to the target gene. Thus, in one embodiment of the invention, the double-stranded RNA construct comprises multiple dsRNA regions, at least one strand of each dsRNA region comprising a nucleotide sequence that is complementary to at least part of a target nucleotide sequence of an insect target gene. According to the invention, the dsRNA regions in the RNA construct may be complementary to the same or to different target genes and/or the dsRNA regions may be complementary to targets from the same or from different insect species.
The terms “hit”, “hits” and “hitting” are alternative wordings to indicate that at least one of the strands of the dsRNA is complementary to, and as such may bind to, the target gene or nucleotide sequence.
In one embodiment, the double stranded RNA region comprises multiple copies of the nucleotide sequence that is complementary to the target gene. Alternatively, the dsRNA hits more than one target sequence of the same target gene. The invention thus encompasses isolated double stranded RNA constructs comprising at least two copies of said nucleotide sequence complementary to at least part of a nucleotide sequence of an insect target.
The term “multiple” in the context of the present invention means at least two, at least three, at least four, at least five, at least six, etc.
The expressions “a further target gene” or “at least one other target gene” mean for instance a second, a third or a fourth, etc. target gene.
DsRNA that hits more than one of the above-mentioned targets, or a combination of different dsRNA against different of the above mentioned targets are developed and used in the methods of the present invention.
Accordingly the invention relates to an isolated double stranded RNA construct comprising at least two copies of the RNA equivalent of at least one of the nucleotide sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or at least two copies of the RNA equivalent of a fragment of at least 17 basepairs in length thereof, preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 basepairs in length thereof. Preferably, said double-stranded RNA comprises the RNA equivalent of the nucleotide sequence as represented in SEQ ID NO 159 or 160, or a fragment of at least 17, preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 basepairs in length thereof. In a further embodiment, the invention relates to an an isolated double stranded RNA construct comprising at least two copies of the RNA equivalent of the nucleotide sequence as represented by SEQ ID NO 159 or 160.
Accordingly, the present invention extends to methods as described herein, wherein the dsRNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of a target nucleotide sequence of an insect target gene, and which comprises the RNA equivalents of at least two nucleotide sequences independently chosen from each other. In one embodiment, the dsRNA comprises the RNA equivalents of at least two, preferably at least three, four or five, nucleotide sequences independently chosen from the sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or fragments thereof of at least 17 basepairs in length, preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 basepairs in length thereof.
The at least two nucleotide sequences may be derived from the target genes herein described. According to one preferred embodiment the dsRNA hits at least one target gene that is essential for viability, growth, development or reproduction of the insect and hits at least one gene involved in pathogenicity or infectivity as described hereinabove. Alternatively, the dsRNA hits multiple genes of the same category, for example, the dsRNA hits at least 2 essential genes or at least 2 genes involved in the same cellular function. According to a further embodiment, the dsRNA hits at least 2 target genes, which target genes are involved in a different cellular function. For example the dsRNA hits two or more genes involved in protein synthesis (e.g. ribosome subunits), intracellular protein transport, nuclear mRNA splicing, or involved in one of the functions described in Table 1A.
Preferably, the present invention extends to methods as described herein, wherein said insect target gene comprises a sequence which is which is selected from the group comprising:

The dsRNA regions (or fragments) in the double stranded RNA may be combined as follows:

- a) when multiple dsRNA regions targeting a single target gene are combined, they may be combined in the original order (ie the order in which the regions appear in the target gene) in the RNA construct,
- b) alternatively, the original order of the fragments may be ignored so that they are scrambled and combined randomly or deliberately in any order into the double stranded RNA construct,
- c) alternatively, one single fragment may be repeated several times, for example from 1 to 10 times, e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 times, in the ds RNA construct, or
- d) the dsRNA regions (targeting a single or different target genes) may be combined in the sense or antisense orientation.

In addition, the target gene(s) to be combined may be chosen from one or more of the following categories of genes:

- e) “essential” genes or “pathogenicity genes” as described above encompass genes that are vital for one or more target insects and result in a lethal or severe (e.g. feeding, reproduction, growth) phenotype when silenced. The choice of a strong lethal target gene results in a potent RNAi effect. In the RNA constructs of the invention, multiple dsRNA regions targeting the same or different (very effective) lethal genes can be combined to further increase the potency, efficacy or speed of the RNAi effect in insect control.
- f) “weak” genes encompass target genes with a particularly interesting function in one of the cellular pathways described herein, but which result in a weak phenotypic effect when silenced independently. In the RNA constructs of the invention, multiple dsRNA regions targeting a single or different weak gene(s) may be combined to obtain a stronger RNAi effect.
- g) “insect specific” genes encompass genes that have no substantial homologous counterpart in non-insect organisms as can be determined by bioinformatics homology searches, for example by BLAST searches. The choice of an insect specific target gene results in a species specific RNAi effect, with no effect or no substantial (adverse) effect in non-target organisms.
- h) “conserved genes” encompass genes that are conserved (at the amino acid level) between the target organism and non-target organism(s). To reduce possible effects on non-target species, such effective but conserved genes are analysed and target sequences from the variable regions of these conserved genes are chosen to be targeted by the dsRNA regions in the RNA construct. Here, conservation is assessed at the level of the nucleic acid sequence. Such variable regions thus encompass the least conserved sections, at the level of the nucleic acid sequence, of the conserved target gene(s).
- i) “conserved pathway” genes encompass genes that are involved in the same biological pathway or cellular process, or encompass genes that have the same functionality in different insect species resulting in a specific and potent RNAi effect and more efficient insect control;
- j) alternatively, the RNA constructs according to the present invention target multiple genes from different biological pathways, resulting in a broad cellular RNAi effect and more efficient insect control.

According to the invention, all double stranded RNA regions comprise at least one strand that is complementary to at least part or a portion of the nucleotide sequence of any of the target genes herein described. However, provided one of the double stranded RNA regions comprises at least one strand that is complementary to a portion of the nucleotide sequence of any one of the target genes herein described, the other double stranded RNA regions may comprise at least one strand that is complementary to a portion of any other insect target gene (including known target genes).
According to yet another embodiment of the present invention there is provided an isolated double stranded RNA or RNA construct as herein described, further comprising at least one additional sequence and optionally a linker. In one embodiment, the additional sequence is chosen from the group comprising (i) a sequence facilitating large-scale production of the dsRNA construct; (ii) a sequence effecting an increase or decrease in the stability of the dsRNA; (iii) a sequence allowing the binding of proteins or other molecules to facilitate uptake of the RNA construct by insects; (iv) a sequence which is an aptamer that binds to a receptor or to a molecule on the surface or in the cytoplasm of an insect to facilitate uptake, endocytosis and/or transcytosis by the insect; or (v) additional sequences to catalyze processing of dsRNA regions. In one embodiment, the linker is a conditionally self-cleaving RNA sequence, preferably a pH sensitive linker or a hydrophobic sensitive linker. In one embodiment, the linker is an intron.
In one embodiment, the multiple dsRNA regions of the double-stranded RNA construct are connected by one or more linkers. In another embodiment, the linker is present at a site in the RNA construct, separating the dsRNA regions from another region of interest. Different linker types for the dsRNA constructs are provided by the present invention.
In another embodiment, the multiple dsRNA regions of the double-stranded RNA construct are connected without linkers.
In a particular embodiment of the invention, the linkers may be used to disconnect smaller dsRNA regions in the pest organism. Advantageously, in this situation the linker sequence may promote division of a long dsRNA into smaller dsRNA regions under particular circumstances, resulting in the release of separate dsRNA regions under these circumstances and leading to more efficient gene silencing by these smaller dsRNA regions. Examples of suitable conditionally self-cleaving linkers are RNA sequences that are self-cleaving at high pH conditions. Suitable examples of such RNA sequences are described by Borda et al. (Nucleic Acids Res. 2003 May 15; 31(10):2595-600), which document is incorporated herein by reference. This sequence originates from the catalytic core of the hammerhead ribozyme HH16.
In another aspect of the invention, a linker is located at a site in the RNA construct, separating the dsRNA regions from another, e.g. the additional, sequence of interest, which preferably provides some additional function to the RNA construct.
In one particular embodiment of the invention, the dsRNA constructs of the present invention are provided with an aptamer to facilitate uptake of the dsRNA by the insect. The aptamer is designed to bind a substance which is taken up by the insect. Such substances may be from an insect or plant origin. One specific example of an aptamer, is an aptamer that binds to a transmembrane protein, for example a transmembrane protein of an insect. Alternatively, the aptamer may bind a (plant) metabolite or nutrient which is taken up by the insect.
Alternatively, the linkers are self-cleaving in the endosomes. This may be advantageous when the constructs of the present invention are taken up by the insect via endocytosis or transcytosis, and are therefore compartmentalized in the endosomes of the insect species. The endosomes may have a low pH environment, leading to cleavage of the linker.
The above mentioned linkers that are self-cleaving in hydrophobic conditions are particularly useful in dsRNA constructs of the present invention when used to be transferred from one cell to another via the transit in a cell wall, for example when crossing the cell wall of an insect pest organism.
An intron may also be used as a linker. An “intron” as used herein may be any non-coding RNA sequence of a messenger RNA. Particular suitable intron sequences for the constructs of the present invention are (1) U-rich (35-45%); (2) have an average length of 100 bp (varying between about 50 and about 500 bp) which base pairs may be randomly chosen or may be based on known intron sequences; (3) start at the 5′ end with -AG:GT- or -CG:GT- and/or (4) have at their 3′ end -AG:GC- or -AG:AA.
A non-complementary RNA sequence, ranging from about 1 base pair to about 10,000 base pairs, may also be used as a linker.
Without wishing to be bound by any particular theory or mechanism, it is thought that long double-stranded RNAs are taken up by the insect from their immediate environment. Double-stranded RNAs taken up into the gut and transferred to the gut epithelial cells are then processed within the cell into short double-stranded RNAs, called small interfering RNAs (siRNAs), by the action of an endogenous endonuclease. The resulting siRNAs then mediate RNAi via formation of a multi-component RNase complex termed the RISC or RNA interfering silencing complex.
In order to achieve down-regulation of a target gene within an insect cell the double-stranded RNA added to the exterior of the cell wall may be any dsRNA or dsRNA construct that can be taken up into the cell and then processed within the cell into siRNAs, which then mediate RNAi, or the RNA added to the exterior of the cell could itself be an siRNA that can be taken up into the cell and thereby direct RNAi.
siRNAs are generally short double-stranded RNAs having a length in the range of from 19 to 25 base pairs, or from 20 to 24 base pairs. In preferred embodiments siRNAs having 19, 20, 21, 22, 23, 24 or 25 base pairs, and in particular 21 or 22 base pairs, corresponding to the target gene to be down-regulated may be used. However, the invention is not intended to be limited to the use of such siRNAs.
siRNAs may include single-stranded overhangs at one or both ends, flanking the double-stranded portion. In a particularly preferred embodiment the siRNA may contain 3′ overhanging nucleotides, preferably two 3′ overhanging thymidines (dTdT) or uridines (UU). 3′ TT or UU overhangs may be included in the siRNA if the sequence of the target gene immediately upstream of the sequence included in double-stranded part of the dsRNA is M. This allows the TT or UU overhang in the siRNA to hybridise to the target gene. Although a 3′ TT or UU overhang may also be included at the other end of the siRNA it is not essential for the target sequence downstream of the sequence included in double-stranded part of the siRNA to have AA. In this context, siRNAs which are RNA/DNA chimeras are also contemplated. These chimeras include, for example, the siRNAs comprising a double-stranded RNA with 3′ overhangs of DNA bases (e.g. dTdT), as discussed above, and also double-stranded RNAs which are polynucleotides in which one or more of the RNA bases or ribonucleotides, or even all of the ribonucleotides on an entire strand, are replaced with DNA bases or deoxynucleotides.
The dsRNA may be formed from two separate (sense and antisense) RNA strands that are annealed together by (non-covalent) basepairing. Alternatively, the dsRNA may have a foldback stem-loop or hairpin structure, wherein the two annealed strands of the dsRNA are covalently linked. In this embodiment the sense and antisense stands of the dsRNA are formed from different regions of single polynucleotide molecule that is partially self-complementary. RNAs having this structure are convenient if the dsRNA is to be synthesised by expression in vivo, for example in a host cell or organism as discussed below, or by in vitro transcription. The precise nature and sequence of the “loop” linking the two RNA strands is generally not material to the invention, except that it should not impair the ability of the double-stranded part of the molecule to mediate RNAi. The features of “hairpin” or “stem-loop” RNAs for use in RNAi are generally known in the art (see for example WO 99/53050, in the name of CSIRO, the contents of which are incorporated herein by reference). In other embodiments of the invention, the loop structure may comprise linker sequences or additional sequences as described above.
Another aspect of the present invention are target nucleotide sequences of the insect target genes herein disclosed. Such target nucleotide sequences are particularly important to design the dsRNA constructs according to the present invention. Such target nucleotide sequences are preferably at least 17, preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 nucleotides in length. Non-limiting examples of preferred target nucleotide sequences are given in the examples.
According to one embodiment, the present invention provides an isolated nucleotide sequence encoding a double stranded RNA or double stranded RNA construct as described herein.
According to a more specific embodiment, the present invention relates to an isolated nucleic acid sequence consisting of a sequence represented by any of SEQ ID NOs 49 to 158, 275 to 472, 533 to 575, 621 to 767, 813 to 862, 908 to 1040, 1161 to 1571, 1730 to 2039, 2120 to 2338, 2384 to 2460, or a fragment of at least 17 preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 nucleotides thereof.
A person skilled in the art will recognize that homologues of these target genes can be found and that these homologues are also useful in the methods of the present invention.
Protein, or nucleotide sequences are likely to be homologous if they show a “significant” level of sequence similarity or more preferably sequence identity. Truely homologous sequences are related by divergence from a common ancestor gene. Sequence homologues can be of two types: (i) where homologues exist in different species they are known as orthologues. e.g. the α-globin genes in mouse and human are orthologues. (ii) paralogues are homologous genes in within a single species. e.g. the α- and β-globin genes in mouse are paralogues
Preferred homologues are genes comprising a sequence which is at least about 85% or 87.5%, still more preferably about 90%, still more preferably at least about 95% and most preferably at least about 99% identical to a sequence selected from the group of sequences represented by SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof. Methods for determining sequence identity are routine in the art and include use of the Blast software and EMBOSS software (The European Molecular Biology Open Software Suite (2000), Rice, P. Longden, I. and Bleasby, A. Trends in Genetics 16, (6) pp 276-277). The term “identity” as used herein refers to the relationship between sequences at the nucleotide level. The expression “% identical” is determined by comparing optimally aligned sequences, e.g. two or more, over a comparison window wherein the portion of the sequence in the comparison window may comprise insertions or deletions as compared to the reference sequence for optimal alignment of the sequences. The reference sequence does not comprise insertions or deletions. The reference window is chosen from between at least 10 contiguous nucleotides to about 50, about 100 or to about 150 nucleotides, preferably between about 50 and 150 nucleotides. “% identity” is then calculated by determining the number of nucleotides that are identical between the sequences in the window, dividing the number of identical nucleotides by the number of nucleotides in the window and multiplying by 100.
Other homologues are genes which are alleles of a gene comprising a sequence as represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481. Further preferred homologues are genes comprising at least one single nucleotide polymorphism (SNIP) compared to a gene comprising a sequence as represented by any of SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159,160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481.
According to another embodiment, the invention encompasses target genes which are insect orthologues of a gene comprising a nucleotide sequence as represented in any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481. By way of example, orthologues may comprise a nucleotide sequence as represented in any of SEQ ID NOs 49 to 123, 275 to 434, 533 to 562, 621 to 738, 813 to 852, 908 to 1010, 1161 to 1437, 1730 to 1987, 2120 to 2290, and 2384 to 2438, or a fragment thereof of at least 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 or 27 nucleotides. A non-limiting list of insect or arachnida orthologues genes or sequences comprising at least a fragment of 17 bp of one of the sequences of the invention, is given in Tables 4.
According to another embodiment, the invention encompasses target genes which are nematode orthologues of a gene comprising a nucleotide sequence as represented in any of 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 248. By way of example, nematode orthologues may comprise a nucleotide sequence as represented in any of SEQ ID NOs 124 to 135, 435 to 446, 563 to 564, 739 to 751, 853, 854, 1011 to 1025, 1438 to 1473, 1988 to 2001, 2291 to 2298, 2439 or 2440, or a fragment of at least 17, 18, 19, 20 or 21 nucleotides thereof. According to another aspect, the invention thus encompasses any of the methods described herein for controlling nematode growth in an organism, or for preventing nematode infestation of an organism susceptible to nematode infection, comprising contacting nematode cells with a double-stranded RNA, wherein the double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of the nucleotide sequence of a target gene comprising a fragment of at least 17, 18, 19, 20 or 21 nucleotides of any of the sequences as represented in SEQ ID NOs 124 to 135, 435 to 446, 563 to 564, 739 to 751, 853, 854, 1011 to 1025, 1438 to 1473, 1988 to 2001, 2291 to 2298, 2439 or 2440, whereby the double-stranded RNA is taken up by the nematode and thereby controls growth or prevents infestation. The invention also relates to nematode-resistant transgenic plants comprising a fragment of at least 17, 18, 19, 20 or 21 nucleotides of any of the sequences as represented in SEQ ID NOs 124 to 135, 435 to 446, 563 to 564, 739 to 751, 853, 854, 1011 to 1025, 1438 to 1473, 1988 to 2001, 2291 to 2298, 2439 or 2440. A non-limiting list of nematode orthologues genes or sequences comprising at least a fragment of 17 bp of one of the sequences of the invention, is given in Tables 5.
According to another embodiment, the invention encompasses target genes which are fungal orthologues of a gene comprising a nucleotide sequence as represented in any of 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481. By way of example, fungal orthologues may comprise a nucleotide sequence as represented in any of SEQ ID NOs 136 to 158, 447 to 472, 565 to 575, 752 to 767, 855 to 862, 1026 to 1040, 1475 to 1571, 2002 to 2039, 2299 to 2338, 2441 to 2460, or a fragment of at least 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 or 27 nucleotides thereof. According to another aspect, the invention thus encompasses any of the methods described herein for controlling fungal growth on a cell or an organism, or for preventing fungal infestation of a cell or an organism susceptible to fungal infection, comprising contacting fungal cells with a double-stranded RNA, wherein the double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of the nucleotide sequence of a target gene comprising a fragment of at least 17, 18, 19, 20 or 21 nucleotides of any of the sequences as represented in SEQ ID NOs 136 to 158, 447 to 472, 565 to 575, 752 to 767, 855 to 862, 1026 to 1040, 1475 to 1571, 2002 to 2039, 2299 to 2338, 2441 to 2460, whereby the double-stranded RNA is taken up by the fungus and thereby controls growth or prevents infestation. The invention also relates to fungal-resistant transgenic plants comprising a fragment of at least 17, 18, 19, 20 or 21 of any of the sequences as represented in SEQ ID NOs 136 to 158, 447 to 472, 565 to 575, 752 to 767, 855 to 862, 1026 to 1040, 1475 to 1571, 2002 to 2039, 2299 to 2338, 2441 to 2460. A non-limiting list of fungal orthologues genes or sequences comprising at least a fragment of 17 bp of one of the sequences of the invention, is given in Tables 6.
In one preferred embodiment of the invention the dsRNA may be expressed by (e.g. transcribed within) a host cell or host organism, the host cell or organism being an organism susceptible or vulnerable to infestation by an insect. In this embodiment RNAi-mediated gene silencing of one or more target genes in the insect may be used as a mechanism to control growth of the insect in or on the host organism and/or to prevent or reduce insect infestation of the host organism. Thus, expression of the double-stranded RNA within cells of the host organism may confer resistance to a particular insect or to a class of insects. In case the dsRNA hits more than one insect target gene, expression of the double-stranded RNA within cells of the host organism may confer resistance to more than one insect or more than one class of insects.
In a preferred embodiment the host organism is a plant and the insect is a plant pathogenic insect. In this embodiment the insect is contacted with the double-stranded RNA by expressing the double-stranded RNA in a plant or plant cell that is infested with or susceptible to infestation with the plant pathogenic insect.
In this context the term “plant” encompasses any plant material that it is desired to treat to prevent or reduce insect growth and/or insect infestation. This includes, inter alia, whole plants, seedlings, propagation or reproductive material such as seeds, cuttings, grafts, explants, etc. and also plant cell and tissue cultures. The plant material should express, or have the capability to express, dsRNA corresponding to one or more target genes of the insect.
Therefore, in a further aspect the invention provides a plant, preferably a transgenic plant, or propagation or reproductive material for a (transgenic) plant, or a plant cell culture expressing or capable of expressing at least one double-stranded RNA, wherein the double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of a target nucleotide sequence of a target gene of an insect, such that the double-stranded RNA is taken up by an insect upon plant-insect interaction, said double stranded RNA being capable of inhibiting the target gene or down-regulating expression of the target gene by RNA interference. The target gene may be any of the target genes herein described, for instance a target gene that is essential for the viability, growth, development or reproduction of the insect.
In this embodiment the insect can be any insect, but is preferably plant pathogenic insect. Preferred plant pathogenic insects include, but are not limited to, those listed above.
A plant to be used in the methods of the invention, or a transgenic plant according to the invention encompasses any plant, but is preferably a plant that is susceptible to infestation by a plant pathogenic insect.
Accordingly, the present invention extends to methods as described herein wherein the plant is chosen from the following group of plants (or crops): alfalfa, apple, apricot, artichoke, asparagus, avocado, banana, barley, beans, beet, blackberry, blueberry, broccoli, brussel sprouts, cabbage, canola, carrot, cassaya, cauliflower, a cereal, celery, cherry, citrus, clemintine, coffee, corn, cotton, cucumber, eggplant, endive, eucalyptus, figes, grape, grapefruit, groundnuts, ground cherry, kiwifruit, lettuce, leek, lemon, lime, pine, maize, mango, melon, millet, mushroom, nut aot, okra, onion, orange, an ornamental plant or flower or tree, papaya, parsley, pea, peach, peanut, peat, pepper, persimmon, pineapple, plantain, plum, pomegranate, potato, pumpkin, radicchio, radish, rapeseed, raspberry, rice, rye, sorghum, soy, soybean, spinach, strawberry, sugarbeet, sugarcane, sunflower, sweet potato, tangerine, tea, tobacco, tomato, a vine, watermelon, wheat, yams and zucchini.
In one embodiment the present invention extends to methods as described herein, wherein the plant is potato and the target gene is a gene from an insect selected from the group consisting of Leptinotarsa spp. (e.g. L. decemlineata (Colorado potato beetle), L. juncta (false potato beetle), or L. texana (Texan false potato beetle)); Lema spp. (e.g. L. trilineata (three-lined potato beetle)); Epitrix spp. (e.g. E. cucumeris (potato flea beetle) or E. tuberis (tuber flea beetle)); Epicauta spp. (e.g. E. vittata (striped blister beetle)); Phaedon spp. (e.g. P. cochleariae (mustard leaf beetle)); Empoasca spp. (e.g. E. fabae (potato leafhopper)); Myzus spp. (e.g. M. persicae (green peach aphid)); Paratrioza spp. (e.g. P. cockerelli (potato psyllid)); Ostrinia spp. (e.g. O. nubilalis (European corn borer)); Conoderus spp. (e.g. C. falli (southern potato wireworm), or C. vespertinus (tobacco wireworm)); and Phthorimaea spp. (e.g. P. operculella (potato tuberworm)); in another embodiment the present invention extends to methods as described herein, wherein the plant is tomato and the target gene is a gene from an insect selected from the group consisting of: Macrosiphum spp. (e.g. M. euphorbiae (potato aphid)); Myzus spp. (e.g. M. persicae (green peach aphid)); Trialeurodes spp. (e.g. T. vaporariorum (greenhouse whitefly), or T. abutilonia (banded-winged whitefly)); Bemisia spp. (e.g. B. argentifolii (silverleaf whitefly)); Frankliniella spp. (e.g. F. occidentalis (western flower thrips)); Leptinotarsa spp. (e.g. L. decemlineata (Colorado potato beetle), L. juncta (false potato beetle), or L. texana (Texan false potato beetle)); Epitrix spp. (e.g. E. hirtipennis (flea beetle)); Lygus spp. (e.g. L. lineolaris (tarnished plant bug), or L. hesperus (western tarnished plant bug)); Euschistus spp. (e.g. E. conspresus (consperse stinkbug)); Nezara spp. (e.g. N. viridula (southern green stinkbug)); Thyanta spp. (e.g. T. pallidovirens (redshouldered stinkbug)); Phthorimaea spp. (e.g. P. operculella (potato tuberworm)); Helicoverpa spp. (e.g. H. zea (tomato fruitworm); Keiferia spp. (e.g. K. lycopersicella (tomato pinworm)); Spodoptera spp. (e.g. S. exigua (beet armyworm), or S. praefica (western yellowstriped armyworm)); Limonius spp. (wireworms); Agrotis spp. (e.g. A. ipsilon (black cutworm)); Manduca spp. (e.g. M. sexta (tobacco hornworm), or M. quinquemaculata (tomato hornworm)); Liriomyza spp. (e.g. L. sativae, L. trifolli or L. huidobrensis (leafminer)); and Paratrioza spp. (e.g. P. cockerelli (tomato psyllid)); In another embodiment the present invention extends to methods as described herein, wherein the plant is corn and the target gene is a gene from an insect selected from the group consisting of: Diabrotica spp. (e.g. D. virgifera virgifera (western corn rootworm), D. barberi (northern corn rootworm), D. undecimpunctata howardi (southern corn rootworm), D. virgifera zeae (Mexican corn rootworm); D. balteata (banded cucumber beetle)); Ostrinia spp. (e.g. O. nubilalis (European corn borer)); Agrotis spp. (e.g. A. ipsilon (black cutworm)); Helicoverpa spp. (e.g. H. zea (corn earworm)); Spodoptera spp. (e.g. S. frugiperda (fall armyworm)); Diatraea spp. (e.g. D. grandiosella (southwestern corn borer), or D. saccharalis (sugarcane borer)); Elasmopalpus spp. (e.g. E. lignosellus (lesser cornstalk borer)); Melanotus spp. (wireworms); Cyclocephala spp. (e.g. C. borealis (northern masked chafer)); Cyclocephala spp. (e.g. C. immaculata (southern masked chafer)); Popillia spp. (e.g. P. japonica (Japanese beetle)); Chaetocnema spp. (e.g. C. pulicaria (corn flea beetle)); Sphenophorus spp. (e.g. S. maidis (maize billbug)); Rhopalosiphum spp. (e.g. R. maidis (corn leaf aphid)); Anuraphis spp. (e.g. A. maidiradicis (corn root aphid)); Blissus spp. (e.g. B. leucopterus leucopterus (chinch bug)); Melanoplus spp. (e.g. M. femurrubrum (redlegged grasshopper), M. sanguinipes (migratory grasshopper)); Hylemya spp. (e.g. H. platura (seedcorn maggot)); Agromyza spp. (e.g. A. parvicomis (corn blot leafminer)); Anaphothrips spp. (e.g. A. obscrurus (grass thrips)); Solenopsis spp. (e.g. S. milesta (thief ant)); and Tetranychus spp. (e.g. T. urticae (twospotted spider mite)); in another embodiment the present invention extends to methods as described herein, wherein the plant is cotton and the target gene is a gene from an insect selected from the group consisting of: Helicoverpa spp. (e.g. H. zea (cotton bollworm)); Pectinophora spp. (e.g. P. gossypiella (pink bollworm)); Helicoverpa spp. (e.g. H. armigera (American bollworm)); Earias spp. (e.g. E. vittella (spotted bollworm)); Heliothis spp. (e.g. H. virescens (tobacco budworm)); Spodoptera spp. (e.g. S. exigua (beet armyworm)); Anthonomus spp. (e.g. A. grandis (boll weevil)); Pseudatomoscelis spp. (e.g. P. seriatus (cotton fleahopper)); Trialeurodes spp. (e.g. T. abutiloneus (banded-winged whitefly) T. vaporariorum (greenhouse whitefly)); Bemisia spp. (e.g. B. argentifolii (silverleaf whitefly)); Aphis spp. (e.g. A. gossypii (cotton aphid)); Lygus spp. (e.g. L. lineolaris (tarnished plant bug) or L. hesperus (western tarnished plant bug)); Euschistus spp. (e.g. E. conspersus (consperse stink bug)); Chlorochroa spp. (e.g. C. sayi (Say stinkbug)); Nezara spp. (e.g. N. viridula (green stinkbug)); Thrips spp. (e.g. T. tabaci (onion thrips)); Franklinkiella spp. (e.g. F. fusca (tobacco thrips), or F. occidentalis (western flower thrips)); Melanoplus spp. (e.g. M. femurrubrum (redlegged grasshopper), or M. differentialis (differential grasshopper)); and Tetranychus spp. (e.g. T. cinnabarinus (carmine spider mite), or T. urticae (twospotted spider mite)); in another embodiment the present invention extends to methods as described herein, wherein the plant is rice and the target gene is a gene from an insect selected from the group consisting of: Nilaparvata spp. (e.g. N. lugens (brown planthopper)); Laodelphax spp. (e.g. L. striatellus (small brown planthopper)); Nephotettix spp. (e.g. N. virescens or N. cincticeps (green leafhopper), or N. nigropictus (rice leafhopper)); Sogatella spp. (e.g. S. furcifera (white-backed planthopper)); Blissus spp. (e.g. B. leucopterus leucopterus (chinch bug)); Scotinophora spp. (e.g. S. vermidulate (rice blackbug)); Acrostemum spp. (e.g. A. hilare (green stink bug)); Pamara spp. (e.g. P. guttata (rice skipper)); Chilo spp. (e.g. C. suppressalis (rice striped stem borer), C. auricilius (gold-fringed stem borer), or C. polychrysus (dark-headed stem borer)); Chilotraea spp. (e.g. C. polychrysa (rice stalk borer)); Sesamia spp. (e.g. S. inferens (pink rice borer)); Tryporyza spp. (e.g. T. innotata (white rice borer)); Tryporyza spp. (e.g T. incertulas (yellow rice borer)); Cnaphalocrocis spp. (e.g. C. medinalis (rice leafroller)); Agromyza spp. (e.g. A. oryzae (leafminer)); Diatraea spp. (e.g. D. saccharalis (sugarcane borer)); Namaga spp. (e.g. N. aenescens (green rice caterpillar)); Xanthodes spp. (e.g. X. transversa (green caterpillar)); Spodoptera spp. (e.g. S. frugiperda (fall armyworm)); Mythimna spp. (e.g. Mythmna (Pseudaletia) seperata (armyworm)); Helicoverpa spp. (e.g. H. zea (corn earworm)); Colaspis spp. (e.g. C. brunnea (grape colaspis)); Lissorhoptrus spp. (e.g. L. oryzophilus (rice water weevil)); Echinocnemus spp. (e.g. E. squamos (rice plant weevil)); Diclodispa spp. (e.g. D. armigera (rice hispa)); Oulema spp. (e.g. O. oryzae (leaf beetle); Sitophilus spp. (e.g. S. oryzae (rice weevil)); Pachydiplosis spp. (e.g. P. oryzae (rice gall midge)); Hydrellia spp. (e.g. H. griseola (small rice leafminer)); Chlorops spp. (e.g. C. oryzae (stem maggot)); and Hydrellia spp. (e.g. H. sasakii (rice stem maggot));
Transgenic plants according to the invention extend to all plant species specifically described above being resistant to the respective insect species as specifically described above. Preferred transgenic plants (or reproductive or propagation material for a transgenic plant, or a cultured transgenic plant cell) are plants (or reproductive or propagation material for a transgenic plant, or a cultured transgenic plant cell) wherein said plant comprises a nucleic acid sequence which is selected from the group comprising:

- (i) sequences which are at least 75% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof, and
- (ii) sequences comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof,

or wherein said nucleic acid is an insect orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 49 to 158, 275 to 472, 533 to 575, 621 to 767, 813 to 862, 908 to ˜1040, 1161 to 1571, 1730 to 2039, 2120 to 2338, 2384 to 2460, or the complement thereof.
The present invention also encompasses plants (or reproductive or propagation material for a transgenic plant, or a cultured transgenic plant cell) which express or are capable of expressing at least one of the nucleotides of the invention, for instance at least one of the nucleotide sequences represented in any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159,160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof, or comprising a fragment thereof comprising at least 17, preferably at least 18, 19, 20 or 21, more preferably at least 22, 23 or 24 nucleotides.
The plant may be provided in a form wherein it is actively expressing (transcribing) the double-stranded RNA in one or more cells, cell types or tissues. Alternatively, the plant may be “capable of expressing”, meaning that it is transformed with a transgene which encodes the desired dsRNA but that the transgene is not active in the plant when (and in the form in which) the plant is supplied.
Therefore, according to another embodiment, a recombinant DNA construct is provided comprising the nucleotide sequence encoding the dsRNA or dsRNA construct according to the present invention operably linked to at least one regulatory sequence. Preferably, the regulatory sequence is selected from the group comprising constitutive promoters or tissue specific promoters as described below.
The target gene may be any target gene herein described. Preferably the regulatory element is a regulatory element that is active in a plant cell. More preferably, the regulatory element is originating from a plant. The term “regulatory sequence” is to be taken in a broad context and refers to a regulatory nucleic acid capable of effecting expression of the sequences to which it is operably linked.
Encompassed by the aforementioned term are promoters and nucleic acids or synthetic fusion molecules or derivatives thereof which activate or enhance expression of a nucleic acid, so called activators or enhancers. The term “operably linked” as used herein refers to a functional linkage between the promoter sequence and the gene of interest, such that the promoter sequence is able to initiate transcription of the gene of interest.
By way of example, the transgene nucleotide sequence encoding the double-stranded RNA could be placed under the control of an inducible or growth or developmental stage-specific promoter which permits transcription of the dsRNA to be turned on, by the addition of the inducer for an inducible promoter or when the particular stage of growth or development is reached.
Alternatively, the transgene encoding the double-stranded RNA is placed under the control of a strong constitutive promoter such as any selected from the group comprising the CaMV35S promoter, doubled CaMV35S promoter, ubiquitin promoter, actin promoter, rubisco promoter, GOS2 promoter, Figwort mosaic viruse (FMV) 34S promoter, cassaya vein mosaic virus (CsVMV) promoter (Verdaguer B. et al, Plant Mol. Biol. 1998 37(6):1055-67).
Alternatively, the transgene encoding the double-stranded RNA is placed under the control of a tissue specific promoter such as any selected from the group comprising root specific promoters of genes encoding PsMTA Class III chitinase, photosynthetic tissue-specific promoters such as promoters of cab1 and cab2, rbcS, gapA, gapB and ST-LS1 proteins, JAS promoters, chalcone synthase promoter and promoter of RJ39 from strawberry.
In another embodiment, the transgene encoding the double-stranded RNA is placed under the control of an insect-induced promoter, for instance the potato proteinase inhibitor II (PinII) promoter (Duan X et al, Nat. Biotechnol. 1996, 14(4):494-8)); or a wounding-induced promoter, for instance the jasmonates and ethylene induced promoters, PDF1.2 promoter (Manners J M et al., Plant Mol. Biol. 1998, 38(6):1071-80); or under a defense related promoter, for instance the salicylic acid induced promoters and plant-pathogenesis related protein (PR protein) promoters (PR1 promoter (Cornelissen B J et al., Nucleic Acids Res. 1987, 15(17):6799-811; COMT promoter (Toquin V et al, Plant Mol. Biol. 2003, 52(3):495-509).
Furthermore, when using the methods of the present invention for developing transgenic plants resistant against insects, it might be beneficial to place the nucleic acid encoding the double-stranded RNA according to the present invention under the control of a tissue-specific promoter. In order to improve the transfer of the dsRNA from the plant cell to the pest, the plants could preferably express the dsRNA in a plant part that is first accessed or damaged by the plant pest. In case of plant pathogenic insects, preferred tissues to express the dsRNA are the leaves, stems, roots, and seeds. Therefore, in the methods of the present invention, a plant tissue-preferred promoter may be used, such as a leaf-specific promoter, a stem-specific promoter, a phloem-specific promoter, a xylem-specific promoter, a root-specific promoter, or a seed-specific promoter (sucrose transporter gene AtSUC promoter (Baud S et al., Plant J. 2005, 43(6):824-36), wheat high molecular weight glutenin gene promoter (Robert L S et al., Plant Cell. 1989, 1(6):569-78.)).
Suitable examples of a root specific promoter are PsMTA (Fordam-Skelton, A. P., et al., 1997 Plant Molecular Biology 34: 659-668.) and the Class III Chitinase promoter. Examples of leaf- and stem-specific or photosynthetic tissue-specific promoters that are also photoactivated are promoters of two chlorophyll binding proteins (cab1 and cab2) from sugar beet (Stahl D. J., et al., 2004 BMC Biotechnology 2004 4:31), ribulose-bisphosphate carboxylase (Rubisco), encoded by rbcS (Nomura M. et al., 2000 Plant Mol. Biol. 44: 99-106), A (gapA) and B (gapB) subunits of chloroplast glyceraldehyde-3-phosphate dehydrogenase (Conley T. R. et al. 1994 Mol. Cell. Biol. 19: 2525-33; Kwon H. B. et al. 1994 Plant Physiol. 105: 357-67), promoter of the Solanum tuberosum gene encoding the leaf and stem specific (ST-LS1) protein (Zaidi M. A. et al., 2005 Transgenic Res. 14:289-98), stem-regulated, defense-inducible genes, such as JAS promoters (patent publication no. 20050034192/US-A1). An example of a flower-specific promoter is for instance, the chalcone synthase promoter (Faktor O. et al. 1996 Plant Mol. Biol. 32: 849) and an example of a fruit-specific promoter is for instance RJ39 from strawberry (WO 98 31812).
In yet other embodiments of the present invention, other promoters useful for the expression of dsRNA are used and include, but are not limited to, promoters from an RNA Poll, an RNA Poll, an RNA PolIII, T7 RNA polymerase or SP6 RNA polymerase. These promoters are typically used for in vitro-production of dsRNA, which dsRNA is then included in an antiinsecticidal agent, for example, in an anti-insecticidal liquid, spray or powder.
Therefore, the present invention also encompasses a method for generating any of the double-stranded RNA or RNA constructs of the invention. This method comprises the steps of

- a. contacting an isolated nucleic acid or a recombinant DNA construct of the invention with cell-free components; or
- b. introducing (e.g. by transformation, transfection or injection) an isolated nucleic acid or a recombinant DNA construct of the invention in a cell, under conditions that allow transcription of said nucleic acid or recombinant DNA construct to produce the dsRNA or RNA construct.

Optionally, one or more transcription termination sequences may also be incorporated in the recombinant construct of the invention. The term “transcription termination sequence” encompasses a control sequence at the end of a transcriptional unit, which signals 3′ processing and poly-adenylation of a primary transcript and termination of transcription. Additional regulatory elements, such as transcriptional or translational enhancers, may be incorporated in the expression construct.
The recombinant constructs of the invention may further include an origin of replication which is required for maintenance and/or replication in a specific cell type. One example is when an expression construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g. plasmid or cosmid molecule) in a cell. Preferred origins of replication include, but are not limited to, f1-ori and colE1 ori.
The recombinant construct may optionally comprise a selectable marker gene. As used herein, the term “selectable marker gene” includes any gene, which confers a phenotype on a cell in which it is expressed to facilitate the identification and/or selection of cells, which are transfected or transformed, with an expression construct of the invention. Examples of suitable selectable markers include resistance genes against ampicillin (Amp^r), tetracycline (Tcr), kanamycin (Kan^r), phosphinothricin, and chloramphenicol (CAT) gene. Other suitable marker genes provide a metabolic trait, for example manA. Visual marker genes may also be used and include for example beta-glucuronidase (GUS), luciferase and Green Fluorescent Protein (GFP).
Plants that have been stably transformed with a transgene encoding the dsRNA may be supplied as seed, reproductive material, propagation material or cell culture material which does not actively express the dsRNA but has the capability to do so.
Accordingly, the present invention encompasses a plant (e.g. a rice plant), or a seed (e.g. a rice seed), or a cell (e.g. a bacterial or plant cell), comprising at least one double-stranded RNA or at least one double-stranded RNA construct as described herein: or at least one nucleotide sequence or at least one recombinant DNA construct as described herein; or at least one plant cell as described herein. The present invention also encompasses a plant (e.g. an alfalfa, apple, apricot, artichoke, asparagus, avocado, banana, barley, beans, beet, blackberry, blueberry, broccoli, brussel sprouts, cabbage, canola, carrot, cassaya, cauliflower, a cereal, celery, cherry, citrus, clemintine, coffee, corn, cotton, cucumber, eggplant, endive, eucalyptus, figes, grape, grapefruit, groundnuts, ground cherry, kiwifruit, lettuce, leek, lemon, lime, pine, maize, mango, melon, millet, mushroom, nut aot, okra, onion, orange, an ornamental plant or flower or tree, papaya, parsley, pea, peach, peanut, peat, pepper, persimmon, pineapple, plantain, plum, pomegranate, potato, pumpkin, radicchio, radish, rapeseed, raspberry, rice, rye, sorghum, soy, soybean, spinach, strawberry, sugarbeet, sugarcane, sunflower, sweet potato, tangerine, tea, tobacco, tomato, a vine, watermelon, wheat, yams or zucchiniplant; preferably a potato, eggplant, tomato, pepper, tobacco, ground cherry, rice corn or cotton plant), or a seed or tuber (e.g. an alfalfa, apple, apricot, artichoke, asparagus, avocado, banana, barley, beans, beet, blackberry, blueberry, broccoli, brussel sprouts, cabbage, canola, carrot, cassaya, cauliflower, a cereal, celery, cherry, citrus, clemintine, coffee, corn, cotton, cucumber, eggplant, endive, eucalyptus, figes, grape, grapefruit, groundnuts, ground cherry, kiwifruit, lettuce, leek, lemon, lime, pine, maize, mango, melon, millet, mushroom, nut aot, okra, onion, orange, an ornamental plant or flower or tree, papaya, parsley, pea, peach, peanut, peat, pepper, persimmon, pineapple, plantain, plum, pomegranate, potato, pumpkin, radicchio, radish, rapeseed, raspberry, rice, rye, sorghum, soy, soybean, spinach, strawberry, sugarbeet, sugarcane, sunflower, sweet potato, tangerine, tea, tobacco, tomato, a vine, watermelon, wheat, yams or zucchini plant; preferably a potato, eggplant, tomato, pepper, tobacco, ground cherry, rice, corn or cotton seed or tuber), or a cell (e.g. a bacterial or plant cell), comprising at least one double-stranded RNA or at least one double-stranded RNA construct as described herein: or at least one nucleotide sequence or at least one recombinant DNA construct as described herein. Preferably, these plants or seeds or cells comprise a recombinant construct wherein the nucleotide sequence encoding the dsRNA or dsRNA construct according to the present invention is operably linked to at least one regulatory element as described above.
The plant may be provided in a form wherein it is actively expressing (transcribing) the RNA molecule in one or more cells, cell types or tissues. Alternatively, the plant may be “capable of expressing”, meaning that it is transformed with a transgene which encodes the desired RNA molecule but that the transgene is not active in the plant when (and in the form in which) the plant is supplied.
In one particular embodiment, there is provided a recombinant (expression) construct for expression of an RNA molecule in a plant or in a plant cell comprising at least one regulatory sequence operably linked to a nucleic acid molecule comprising at least 14, 15, 16, 17, 18, 19, 20, 21, 22 etc. nucleotides, up to all of the nucleotides of the sequence set forth as SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or comprising at least 14, 15, 16, 17, 18, 19, 20, 21, 22 etc. up to all nucleotides of the sequence of an orthologous nucleic acid molecule from a different target species. Many vectors are available for this purpose, and selection of the appropriate vector will depend mainly on the size of the nucleic acid to be inserted into the vector and the particular host cell to be transformed with the vector.
General techniques for expression of exogenous double-stranded RNA in plants for the purposes of RNAi are known in the art (see Baulcombe D, 2004, Nature. 431(7006):356-63. RNA silencing in plants, the contents of which are incorporated herein by reference). More particularly, methods for expression of double-stranded RNA in plants for the purposes of down-regulating gene expression in plant pests such as nematodes or insects are also known in the art. Similar methods can be applied in an analogous manner in order to express double-stranded RNA in plants for the purposes of down-regulating expression of a target gene in a plant pathogenic insect. In order to achieve this effect it is necessary only for the plant to express (transcribe) the double-stranded RNA in a part of the plant which will come into direct contact with the insect, such that the double-stranded RNA can be taken up by the insect. Depending on the nature of the insect and its relationship with the host plant, expression of the dsRNA could occur within a cell or tissue of a plant within which the insect is also present during its life cycle, or the RNA may be secreted into a space between cells, such as the apoplast, that is occupied by the insect during its life cycle. Furthermore, the dsRNA may be located in the plant cell, for example in the cytosol, or in the plant cell organelles such as a chloroplast, mitochondrion, vacuole or endoplastic reticulum.
Alternatively, the dsRNA may be secreted by the plant cell and by the plant to the exterior of the plant. As such, the dsRNA may form a protective layer on the surface of the plant.
In a further aspect, the invention also provides combinations of methods and compositions for preventing or protecting plants from pest infestation. For instance, one means provides using the plant transgenic approach combining methods using expression of dsRNA molecules and methods using expression of such Bt insecticidal proteins.
Therefore the invention also relates to a method or a plant cell or plant described herein, wherein said plant cell or plant expressing said RNA molecule comprises or expresses a pesticidal agent selected from the group consisting of a patatin, a Bacillus thuringiensis insecticidal protein, a Xenorhabdus insecticidal protein, a Photorhabdus insecticidal protein, a Bacillus laterosporous insecticidal protein, and a Bacillus sphearicus insecticidal protein. Preferably said Bacillus thuringiensis insecticidal protein is selected from the group consisting of a Cry1, a Cry3, a TIC851, a CryET170, a Cry22, a binary insecticidal protein CryET33 and CryET34, a binary insecticidal protein CryET80 and CryET76, a binary insecticidal protein TIC100 and TIC101, and a binary insecticidal protein PS149B1.
In a further embodiment, the invention relates to a composition for controlling insect growth and/or preventing or reducing insect infestation, comprising at least a plant part, plant cell, plant tissue or seed comprising at least one double-stranded RNA, wherein said double-stranded RNA comprises annealed complementary strands, one of which has a nucleotide sequence which is complementary to at least part of a nucleotide sequence of an insect target gene. Optionally, the composition further comprises at least one suitable carrier, excipient or diluent. The target gene may be any target gene described herein. Preferably the insect target gene is essential for the viability, growth, development or reproduction of the insect.
In another aspect the invention relates to a composition as described above, wherein the insect target gene comprises a sequence which is at least 75%, preferably at least 80%, 85%, 90%, more preferably at least 95%, 98% or 99% identical to a sequence selected from the group of sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof, or wherein said insect target gene is an insect orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof.
According to a still further embodiment, the present invention extends to a method for increasing plant yield comprising introducing in a plant any of the nucleotide sequences or recombinant DNA constructs as herein described in an expressible format. Plants encompassed by this method are as described earlier.
The invention will be further understood with reference to the following non-limiting examples.

BRIEF DESCRIPTION OF FIGURES AND TABLES

FIG. 1-LD: Survival of L. decemlineata on artificial diet treated with dsRNA. Insects of the second larval stage were fed diet treated with 50 μl of topically-applied solution of dsRNA (targets or gfp control). Diet was replaced with fresh diet containing topically-applied dsRNA after 7 days. The number of surviving insects were assessed at

days

2, 5, 7, 8, 9, & 13. The percentage of surviving larvae was calculated relative to day 0 (start of assay). Target LD006: (SEQ ID NO 178); Target LD007 (SEQ ID NO 183); Target LD010 (SEQ ID NO 188); Target LD011 (SEQ ID NO 193); Target LD014 (SEQ ID NO 198); gfp dsRNA (SEQ ID NO 235).

FIG. 2-LD: Survival of L. decemlineata on artificial diet treated with dsRNA. Insects of the second larval stage were fed diet treated with 50 μl of topically-applied solution of dsRNA (targets or gfp control). Diet was replaced with fresh diet only after 7 days. The number of surviving insects was assessed at

days

2, 5, 6, 7, 8, 9, 12, & 14. The percentage of surviving larvae was calculated relative to day 0 (start of assay). Target LD001 (SEQ ID NO 163); Target LD002 (SEQ ID NO 168); Target LD003 (SEQ ID NO 173); Target LD015 (SEQ ID NO 215); Target LD016 (SEQ ID NO 220); gfp dsRNA (SEQ ID NO 235).

FIG. 3-LD: Average weight of L. decemlineata larvae on potato leaf discs treated with dsRNA. Insects of the second larval stage were fed leaf discs treated with 20 μl of a topically-applied solution (10 ng/μl) of dsRNA (target LD002 or gfp). After two days the insects were transferred on to untreated leaves every day.

FIG. 4-LD: Survival of L. decemlineata on artificial diet treated with shorter versions of target LD014 dsRNA and concatemer dsRNA. Insects of the second larval stage were fed diet treated with 50 μl of topically-applied solution of dsRNA (gfp or targets). The number of surviving insects were assessed at

days

3, 4, 5, 6, & 7. The percentage of surviving larvae were calculated relative to day 0 (start of assay).

FIG. 5-LD: Survival of L. decemlineata larvae on artificial diet treated with different concentrations of dsRNA of target LD002 (a), target LD007 (b), target LD010 (c), target LD011 (d), target LD014 (e), target LD015 (f), LD016 (g) and target LD027 (h). Insects of the second larval stage were fed diet treated with 50 μl of topically-applied solution of dsRNA. Diet was replaced with fresh diet containing topically-applied dsRNA after 7 days. The number of surviving insects were assessed at regular intervals. The percentage of surviving larvae were calculated relative to day 0 (start of assay).

FIG. 6-LD. Survival of L. decemlineata adults on potato leaf discs treated with dsRNA. Young adult insects were fed double-stranded-RNA-treated leaf discs for the first two days and were then placed on untreated potato foliage. The number of surviving insects were assessed regularly; mobile insects were recorded as insects which were alive and appeared to move normally; moribund insects were recorded as insects which were alive but appeared sick and slow moving—these insects were not able to right themselves once placed on their backs. Target LD002 (SEQ ID NO 168); Target LD010 (SEQ ID NO 188); Target LD014 (SEQ ID NO 198); Target LD016 (SEQ ID NO 220); gfp dsRNA (SEQ ID NO 235).

FIG. 7-LD. Mortality and growth/developmental delay of larval survivors of the Colorado potato beetle, Leptinotarsa decemlineata, on transgenic potato plants. Seven CPB L1 larvae were fed on transgenic potato siblings harbouring LD002 construct (), empty vector (▴), or wild type line V plants (▪) for seven days. Mortality is expressed in percentage and average larval weight in mg.

FIG. 1-PC: Effects of ingested target dsRNAs on survival and growth of P. cochleariae larvae. Neonate larvae were fed oilseed rape leaf discs treated with 25 μl of topically-applied solution of 0.1 μg/μl dsRNA (targets or gfp control). After 2 days, the insects were transferred onto fresh dsRNA-treated leaf discs. At day 4, larvae from one replicate for every treatment were collected and placed in a Petri dish containing fresh untreated oilseed rape foliage. The insects were assessed at

days

2, 4, 7, 9 & 11. (a) Survival of E. varivestis larvae on oilseed rape leaf discs treated with dsRNA. The percentage of surviving larvae was calculated relative to day 0 (start of assay). (b) Average weights of P. cochleariae larvae on oilseed rape leaf discs treated with dsRNA. Insects from each replicate were weighed together and the average weight per larva determined. Error bars represent standard deviations. Target 1: SEQ ID NO 473; target 3: SEQ ID NO 478; target 5: SEQ ID NO 483--; target 10: SEQ ID NO 488; target 14: SEQ ID NO 493; target 16: SEQ ID NO 498; target 27: SEQ ID NO 503; gfp dsRNA: SEQ ID NO 235.

FIG. 2-PC: Survival of P. cochleariae on oilseed rape leaf discs treated with different concentrations of dsRNA of (a) target PC010 and (b) target PC027. Neonate larvae were placed on leaf discs treated with 25 μl of topically-applied solution of dsRNA. Insects were transferred to fresh treated leaf discs at day 2. At day 4 for target PC010 and day 5 for target PC027, the insects were transferred to untreated leaves. The number of surviving insects were assessed at

days

2, 4, 7, 8, 9 & 11 for PC010 and 2, 5, 8, 9 & 12 for PC027. The percentage of surviving larvae was calculated relative to day 0 (start of assay).

FIG. 1-EV: Survival of E. varivestis larvae on bean leaf discs treated with dsRNA. Neonate larvae were fed bean leaf discs treated with 25 μl of topically-applied solution of 1 μg/μl dsRNA (targets or gfp control). After 2 days, the insects were transferred onto fresh dsRNA-treated leaf discs. At day 4, larvae from one treatment were collected and placed in a plastic box containing fresh untreated bean foliage. The insects were assessed for mortality at

days

2, 4, 6, 8 & 10. The percentage of surviving larvae was calculated relative to day 0 (start of assay). Target 5: SEQ ID NO 576; target 10: SEQ ID NO 586; target 15: SEQ ID NO 591; target 16: SEQ ID NO 596; gfp dsRNA: SEQ ID NO 235.

FIG. 2-EV: Effects of ingested target dsRNAs on survival of E. varivestis adults and resistance to snap bean foliar insect damage. (a) Survival of E. vanvestis adults on bean leaf treated with dsRNA. Adults were fed bean leaf discs treated with 75 μl of topically-applied solution of 0.1 μg/μl dsRNA (targets or gfp control). After 24 hours, the insects were transferred onto fresh dsRNA-treated leaf discs. After a further 24 hours, adults from one treatment were collected and placed in a plastic box containing potted fresh untreated whole bean plants. The insects were assessed for mortality at

days

4, 5, 6, 7, 8, & 11. The percentage of surviving adults was calculated relative to day 0 (start of assay). Target 10: SEQ ID NO 586; target 15: SEQ ID NO 591; target 16: SEQ ID NO 596; gfp dsRNA: SEQ ID NO 235. (b) Resistance to bean foliar damage caused by adults of the E. varivestis by dsRNA. Whole plants containing insects from one treatment (see (a)) were checked visually for foliar damage on day 9. (i) target 10; (ii) target 15; (iii) target 16; (iv) gfp dsRNA; (v) untreated.

FIG. 1-TC: Survival of T. castaneum larvae on artificial diet treated with dsRNA of target 14. Neonate larvae were fed diet based on a flour/milk mix with 1 mg dsRNA target 14. Control was water (without dsRNA) in diet. Four replicates of 10 first instar larvae per replicate were performed for each treatment. The insects were assessed for survival as average percentage means at

days

6, 17, 31, 45 and 60. The percentage of surviving larvae was calculated relative to day 0 (start of assay). Error bars represent standard deviations. Target TC014: SEQ ID NO 878.

FIG. 1-MP: Effect of ingested target 27 dsRNA on the survival of Myzus persicae nymphs. First instars were placed in feeding chambers containing 50 μl of liquid diet with 2 μg/μl dsRNA (target 27 or gfp dsRNA control). Per treatment, 5 feeding chambers were set up with 10 instars in each feeding chamber. Number of survivors were assessed at 8 days post start of bioassay. Error bars represent standard deviations. Target MP027: SEQ ID NO 1061; gfp dsRNA: SEQ ID NO 235.

FIG. 1-NL: Survival of Nilaparvata lugens on liquid artificial diet treated with dsRNA. Nymphs of the first to second larval stage were fed diet supplemented with 2 mg/ml solution of dsRNA targets in separate bioassays: (a) NL002, NL003, NL005, NL010; (b) NL009, NL016; (c) NL014, NL018; (d) NL013, NL015, NL021. Insect survival on targets were compared to diet only and diet with gfp dsRNA control at same concentration. Diet was replaced with fresh diet containing dsRNA every two days. The number of surviving insects were assessed every day

FIG. 2-NL: Survival of Nilaparvata lugens on liquid artificial diet treated with different concentrations of target dsRNA NL002. Nymphs of the first to second larval stage were fed diet supplemented with 1, 0.2, 0.08, and 0.04 mg/ml (final concentration) of NL002. Diet was replaced with fresh diet containing dsRNA every two days. The numbers of surviving insects were assessed every day.

EXAMPLES

Example 1

Silencing C. elegans Target Genes in C. elegans in High Throughput Screening

A C. elegans genome wide library was prepared in the pGN9A vector (WO 01/88121) between two identical T7-promoters and terminators, driving its expression in the sense and antisense direction upon expression of the T7 polymerase, which was induced by IPTG.
This library was transformed into the bacterial strain AB301-105 (DE3) in 96 well plate format. For the genome wide screening, these bacterial cells were fed to the nuclease deficient C. elegans nuc-1(e1392) strain.
Feeding the dsRNA produced in the bacterial strain AB301-105 (DE3), to C. elegans nuc-1 (e1392) worms, was performed in a 96 well plate format as follows: nuc-1 eggs were transferred to a separate plate and allowed to hatch simultaneously at 20° C. for synchronization of the L1 generation. 96 well plates were filled with 100 μL liquid growth medium comprising IPTG and with 10 μL bacterial cell culture of OD6001 AB301-105 (DE3) of the C. elegans dsRNA library carrying each a vector with a C. elegans genomic fragment for expression of the dsRNA. To each well, 4 of the synchronized L1 worms were added and were incubated at 25° C. for at least 4 to 5 days. These experiments were performed in quadruplicate. In the screen 6 controls were used:
pGN29=negative control, wild type
pGZ1=unc-22=twitcher phenotype
pGZ18=chitin synthase=embryonic lethal
pGZ25=pos-1=embryonic lethal
pGZ59=bli-4D=acute lethal
ACC=acetyl co-enzym A carboxylase=acute lethal
After 5 days, the phenotype of the C. elegans nuc-1 (e1392) worms fed with the bacteria producing dsRNA were compared to the phenotype of worms fed with the empty vector (pGN29) and the other controls. The worms that were fed with the dsRNA were screened for lethality (acute or larval) lethality for the parent (Po) generation, (embryonic) lethality for the first filial (F1) generation, or for growth retardation of Po as follows: (i) Acute lethality of Po: L1's have not developed and are dead, this phenotype never gives progeny and the well looks quite empty; (ii) (Larval) lethality of Po: Po died in a later stage than L1, this phenotype also never gives progeny. Dead larvae or dead adult worms are found in the wells; (iii) Lethality for F1: L1's have developed until adult stage and are still alive. This phenotype has no progeny. This can be due to sterility, embryonic lethality (dead eggs on the bottom of well), embryonic arrest or larval arrest (eventually ends up being lethal): (iv) Arrested in growth and growth retardation/delay: Compared to a well with normal development and normal # of progeny.
For the target sequences presented in Table 1A, it was concluded that dsRNA mediated silencing of the C. elegans target gene in nematodes, such as C. elegans, had a fatal effect on the growth and viability of the worm.
Subsequent to the above dsRNA silencing experiment, a more detailed phenotyping experiment was conducted in C. elegans in a high throughput format on 24 well plates. The dsRNA library produced in bacterial strain AB301-105 (DE3), as described above, was fed to C. elegans nuc-1 (e1392) worms on 24 well plates as follows: nuc-1 eggs were transferred to a separate plate and allowed to hatch simultaneously at 20 C for synchronization of the L1 generation. Subsequently 100 of the synchronized L1 worms were soaked in a mixture of 500 μL S-complete fed medium, comprising 5 μg/mL cholesterol, 4 μL/mL PEG and 1 mM IPTG, and 500 μL of bacterial cell culture of OD ₆₀₀1 AB301-105 (DE3) of the C. elegans dsRNA library carrying each a vector with a C. elegans genomic fragment for expression of the dsRNA. The soaked L1 worms were rolled for 2 hours at 25° C.
After centrifugation and removal of 950 μL of the supernatant, 5 μL of the remaining and resuspended pellet (comprising about 10 to 15 worms) was transferred in the middle of each well of a 24 well plate, filled with a layer of agar LB broth. The inoculated plate was incubated at 25° C. for 2 days. At the adult stage, 1 adult worm was singled and incubated at 25° C. for 2 days for inspection of its progeny. The other adult worms are inspected in situ on the original 24 well plate. These experiments were performed in quadruplicate.
This detailed phenotypic screen was repeated with a second batch of worms, the only difference being that the worms of the second batch were incubated at 20 C for 3 days.
The phenotype of the worms fed with C. elegans dsRNA was compared to the phenotype of C. elegans nuc-1 (e1392) worms fed with the empty vector.
Based on this experiment, it was concluded that silencing the C. elegans target genes as represented in Table 1A had a fatal effect on the growth and viability of the worm and that the target gene is essential to the viability of nematodes. Therefore these genes are good target genes to control (kill or prevent from growing) nematodes via dsRNA mediated gene silencing. Accordingly, the present invention encompasses the use of nematode orthologues of the above C. elegans target gene, to control nematode infestation, such as nematode infestation of plants.

Example 2

Identification of D. melanogaster Orthologues

As described above in Example 1, numerous C. elegans lethal sequences were identified and can be used for identifying orthologues in other species and genera. For example, the C. elegans lethal sequences can be used to identify orthologous D. melanogasters sequences. That is, each C. elegans sequence can be querried against a public database, such as GenBank, for orthologous sequences in D. melanogaster. Potential D. melanogaster orthologues were selected that share a high degree of sequence homology (E value preferably less than or equal to 1E-30) and the sequences are blast reciprocal best hits, the latter means that the sequences from different organisms (e.g. C. elegans and D. melanogaster) are each other's top blast hits. For example, sequence C from C. elegans is compared against sequences in D. melanogaster using BLAST. If sequence C has the D. melanogaster sequence D as best hit and when D is compared to all the sequences of C. elegans, also turns out to be sequence C, then D and C are reciprocal best hits. This criterium is often used to define orthology, meaning similar sequences of different species, having similar function. The D. melanogaster sequence identifiers are represented in Table 1A.

Example 3

Leptinotarsa decemlineata (Colorado Potato Beetle)

A. Cloning Partial Gene Sequences from Leptinotarsa decemlineata
High quality, intact RNA was isolated from 4 different larval stages of Leptinotarsa decemlineata (Colorado potato beetle; source: Jeroen van Schaik, Entocare CV Biologische Gewasbescherming, Postbus 162, 6700 AD Wageningen, the Netherlands) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manufacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the LD001, LD002, LD003, LD006, LD007, LD010, LD011, LD014, LD015, LD016, LC018 and LD027 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manufacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-LD, which displays Leptintarsa decemlineata target genes including primer sequences and cDNA sequences obtained. These primers were used in respective PCR reactions with the following conditions: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/topo vector (Cat. No. K2500 20, Invitrogen), and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-LD and are referred to as the partial sequences. The corresponding partial amino acid sequence are represented by the respective SEQ ID NOs as given in Table 3-LD, where the start of the reading frame is indicated in brackets.
B. dsRNA Production of the Leptinotarsa decemlineata Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-LD. The conditions in the PCR reactions were as follows: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-LD. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-LD. Table 8-LD displays sequences for preparing ds RNA fragments of Leptinotarsa decemlineata target sequences and concatemer sequences, including primer sequences.
C. Cloning Leptinotarsa decemlineata Genes into Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, were cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs were generated using the LR recombination reaction between an attL-containing entry clone (see Example 1) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) was obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction was performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments resulted in a hairpin construct for each of the LD002, LD006, LD007, LD010, LD011, LD014 and LD016 genes, having either the promoter-sense-intron-CmR-intron-antisense orientation, or promoter-antisense-intron-CmR-intron-sense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
For LD002 and LD010, a double digest with restriction enzymes BsoBI & PvuI was done on LD002 cloned into pCR8/GW/topo (see Example 3A). For LD006, LD007, LD011, LD014, LD016 and LD027, a digest with restriction enzyme BsoBI was done on LD006 cloned into pCR8/GW/topo (see Example 3A). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) was purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) was added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix was transformed into Top 10 chemically competent cells. Positive clones were selected by restriction digest analysis. The complete sequence of the hairpin construct for:
LD002 (antisense-intron-CmR-intron-sense) is set forth in SEQ ID NO 240;
LD006 (sense-intron-CmR-intron-antisense) is set forth in SEQ ID NO 241;
LD007 sense-intron-CmR-intron-antisense) is set forth in SEQ ID NO 242;
LD010 (antisense-intron-CmR-intron-sense) is set forth in SEQ ID NO 243;
LD011 (sense-intron-CmR-intron-antisense) is set forth in SEQ ID NO 244;
LD014 (sense-intron-CmR-intron-antisense) is set forth in SEQ ID NO 245;
LD016 (antisense-intron-CmR-intron-sense) is set forth in SEQ ID NO 246;
LD027 (sense-intron-CmR-intron-antisense) is set forth in SEQ ID NO 2486.
Table 9-LD provides complete sequences for each hairpin construct.
D. Screening dsRNA Targets Using Artificial Diet for Activity Against Leptinotarsa decemlineata
Artificial diet for the Colorado potato beetle was prepared as follows (adapted from Gelman et al., 2001, J. Ins. Sc., vol. 1, no. 7, 1-10): water and agar were autoclaved, and the remaining ingredients (shown in Table A below) were added when the temperature dropped to 55° C. At this temperature, the ingredients were mixed well before the diet was aliquoted into 24-well plates (Nunc) with a quantity of 1 ml of diet per well. The artificial diet was allowed to solidify by cooling at room temperature. Diet was stored at 4° C. for up to three weeks.

TABLE A

Ingredients for Artificial diet

	Ingredients	Volume for 1 L

water	768	ml
agar	14	g
rolled oats	40	g
Torula yeast	60	g
lactalbumin hydrolysate	30	g
casein	10	g
fructose	20	g
Wesson salt mixture	4	g
tomato fruit powder	12.5	g
potato leaf powder	25	g
b-sitosterol	1	g
sorbic acid	0.8	g
methyl paraben	0.8	g
Vanderzant vitamin mix	12	g
neomycin sulfate	0.2	g
aureomycin	0.130	g
rifampicin	0.130	g
chloramphenicol	0.130	g
nystatin	0.050	g
soybean oil
2	ml
wheat germ oil	2	ml

Fifty μl of a solution of dsRNA at a concentration of 1 mg/ml was applied topically onto the solid artificial diet in the wells of the multiwell plate. The diet was dried in a laminair flow cabin. Per treatment, twenty-four Colorado potato beetle larvae (2^ndstage), with two insects per well, were tested. The plates were stored in the insect rearing chamber at 25±2° C., 60% relative humidity, with a 16:8 hours light:dark photoperiod. The beetles were assessed as live or dead every 1, 2 or 3 days. After seven days, for targets LD006, LD007, LD010, LD011, and LD014, the diet was replaced with fresh diet with topically applied dsRNA at the same concentration (1 mg/ml); for targets LD001, LD002, LD003, LD015, and LD016, the diet was replaced with fresh diet only. The dsRNA targets were compared to diet only or diet with topically applied dsRNA corresponding to a fragment of the GFP (green fluorescent protein) coding sequence (SEQ ID NO 235).
Feeding artificial diet containing intact naked dsRNAs to L. decemlineata larvae resulted in significant increases in larval mortalities as indicated in two separate bioassays (FIGS. 1LD-2LD).
All dsRNAs tested resulted ultimately in 100% mortality after 7 to 14 days. Diet with or without GFP dsRNA sustained the insects throughout the bioassays with very little or no mortality.
Typically, in all assays observed, CPB second-stage larvae fed normally on diet with or without dsRNA for 2 days and molted to the third larval stage. At this new larval stage the CPB were observed to reduce significantly or stop altogether their feeding, with an increase in mortality as a result.
E. Bioassay of dsRNA Targets Using Potato Leaf Discs for Activity Against the Leptinotarsa decemlineata
An alternative bioassay method was employed using potato leaf material rather than artificial diet as food source for CPB. Discs of approximately 1.1 cm in diameter (or 0.95 cm²) were cut out off leaves of 2 to 3-week old potato plants using a suitably-sized cork borer. Treated leaf discs were prepared by applying 20 μl of a 10 ng/μl solution of target LD002 dsRNA or control gfp dsRNA on the adaxial leaf surface. The leaf discs were allowed to dry and placed individually in 24 wells of a 24-well multiplate (Nunc). A single second-larval stage CPB was placed into each well, which was then covered with tissue paper and a multiwell plastic lid. The plate containing the insects and leaf discs were kept in an insect chamber at 28° C. with a photoperiod of 16 h light/8 h dark. The insects were allowed to feed on the leaf discs for 2 days after which the insects were transferred to a new plate containing fresh treated leaf discs. Thereafter, the insects were transferred to a plate containing untreated leaf discs every day until day 7. Insect mortality and weight scores were recorded.
Feeding potato leaf discs with surface-applied intact naked dsRNA of target LD002 to L. decemlineata larvae resulted in a significant increase in larval mortalities (i.e. at day 7 all insects were dead; 100% mortality) whereas control gfp dsRNA had no effect on CPB survival. Target LD002 dsRNA severely affected the growth of the larvae after 2 to 3 days whereas the larvae fed with gfp dsRNA at the same concentration developed as normal (FIG. 3-LD).
F. Screening Shorter Versions of dsRNAs Using Artificial Diet for Activity Against Leptinotarsa decemlineata
This example exemplifies the finding that shorter (60 or 100 bp) dsRNA fragments on their own or as concatemer constructs are sufficient in causing toxicity towards the Colorado potato beetle.
LD014, a target known to induce lethality in Colorado potato beetle, was selected for this example. This gene encodes a V-ATPase subunit E (SEQ ID NO 15).
A 100 base pair fragment, LD014_F1, at position 195-294 on SEQ ID NO 15 (SEQ ID NO 159) and a 60 base pair fragment, LD014_F2, at position 235-294 on SEQ ID NO 15 (SEQ ID NO 160) were further selected. See also Table 7-LD.
Two concatemers of 300 base pairs, LD014_C1 and LD014_C2, were designed (SEQ ID NO 161 and SEQ ID NO 162). LD014_C1 contained 3 repeats of the 100 base pair fragment described above (SEQ ID NO 159) and LD014_C2 contained 5 repeats of the 60 base pair fragment described above (SEQ ID NO 160). See also Table 7-LD.
The fragments LD014_F1 and LD014_F2 were synthesized as sense and antisense primers. These primers were annealed to create the double strands DNA molecules prior to cloning. XbaI and XmaI restrictions sites were included at the 5′ and 3′ ends of the primers, respectively, to facilitate the cloning.
The concatemers were made as 300 base pairs synthetic genes. XbaI and XmaI restrictions sites were included at the 5′ and 3′ ends of the synthetic DNA fragments, respectively, to facilite the cloning.
The 4 DNA molecules, i.e. the 2 single units (LD014_F1 & LD014_F2) and the 2 concatemers (LD014_C1 & LD014_C2), were digested with XbaI and XmaI and subcloned in pBluescriptII SK+ linearised by XbaI and XmaI digests, resulting in recombinant plasmids p1, p2, p3, & p4, respectively.
Double-stranded RNA production: dsRNA was synthesized using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter. For LD014_F1, the sense T7 template was generated using the specific T7 forward primer oGBM159 and the specific reverse primer oGBM164 (represented herein as SEQ ID NO 204 and SEQ ID NO 205, respectively) in a PCR reaction with the following conditions: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using the specific forward primer oGBM163 and the specific T7 reverse primer oGBM160 (represented herein as SEQ ID NO 206 and SEQ ID NO 207, respectively) in a PCR reaction with the same conditions as described above. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, Dnase and Rnase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA is herein represented by SEQ ID NO 203.
For LD014_F2, the sense T7 template was generated using the specific T7 forward primer oGBM161 and the specific reverse primer oGBM166 (represented herein as SEQ ID NO 209 and SEQ ID NO 210, respectively) in a PCR reaction with the following conditions: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using the specific forward primer oGBM165 and the specific T7 reverse primer oGBM162 (represented herein as SEQ ID NO 211 and SEQ ID NO 212, respectively) in a PCR reaction with the same conditions as described above. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, Dnase and Rnase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA is herein represented by SEQ ID NO 208.
Also for the concatemers, separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter. The recombinant plasmids p3 and p4 containing LD014_C1 & LD014_C2 were linearised with XbaI or XmaI, the two linear fragments for each construct purified and used as template for the in vitro transcription assay, using the T7 promoters flanking the cloning sites. Double-stranded RNA was prepared by in vitro transcription using the 17 RiboMAX™ Express RNAi System (Promega). The sense strands of the resulting dsRNA for LD014_C1 and LD014_C2 are herein represented by SEQ ID NO 213 and 214, respectively.
Shorter sequences of target LD014 and concatemers were able to induce lethality in Leptinotarsa decemlineata, as shown in FIG. 4-LD.
G. Screening dsRNAs at Different Concentrations Using Artificial Diet for Activity Against Leptinotarsa decemlineata
Fifty μl of a solution of dsRNA at serial ten-fold concentrations from 1 μg/μl (for target LD027 from 0.1 μg/μl) down to 0.01 ng/μl was applied topically onto the solid artificial diet in the wells of a 24-well plate (Nunc). The diet was dried in a laminair flow cabin. Per treatment, twenty-four Colorado potato beetle larvae (2^ndstage), with two insects per well, were tested. The plates were stored in the insect rearing chamber at 25±2° C., 60% relative humidity, with a 16:8 hours light:dark photoperiod. The beetles were assessed as live or dead at regular intervals up to day 14. After seven days, the diet was replaced with fresh diet with topically applied dsRNA at the same concentrations. The dsRNA targets were compared to diet only.
Feeding artificial diet containing intact naked dsRNAs of different targets to L. decemlineata larvae resulted in high larval mortalities at concentrations as low as between 0.1 and 10 ng dsRNA/μl as shown in FIG. 5-LD.
H. Adults are Extremely Susceptible to Orally Ingested dsRNA Corresponding to Target Genes.
The example provided below highlights the finding that adult insects (and not only insects of the larval stage) are extremely susceptible to orally ingested dsRNA corresponding to target genes.
Four targets were chosen for this experiment: targets 2, 10, 14 and 16 (SEQ ID NO 168, 188, 198 and 220, respectively). GFP fragment dsRNA (SEQ ID NO 235) was used as a control. Young adults (2 to 3 days old) were picked at random from our laboratory-reared culture with no bias towards insect gender. Ten adults were chosen per treatment. The adults were prestarved for at least 6 hours before the onset of the treatment. On the first day of treatment, each adult was fed four potato leaf discs (diameter 1.5 cm²) which were pretreated with a topical application of 25 μl of 0.1 μg/μl target dsRNA (synthesized as described in Example 3A; topical application as described in Example 3E) per disc. Each adult was confined to a small petridish (diameter 3 cm) in order to make sure that all insects have ingested equal amounts of food and thus received equal doses of dsRNA. The following day, each adult was again fed four treated leaf discs as described above. On the third day, all ten adults per treatment were collected and placed together in a cage consisting of a plastic box (dimensions 30 cm×20 cm×15 cm) with a fine nylon mesh built into the lid to provide good aeration. Inside the box, some moistened filter paper was placed in the base. Some (untreated) potato foliage was placed on top of the paper to maintain the adults during the experiment. From day 5, regular assessments were carried out to count the number of dead, alive (mobile) and moribund insects. For insect moribundity, adults were laid on their backs to check whether they could right themselves within several minutes; an insect was considered moribund only if it was not able to turn onto its front.
Clear specific toxic effects of double-stranded RNA corresponding to different targets towards adults of the Colorado potato beetle, Leptinotarsa decemlineata, were demonstrated in this experiment (FIG. 6-LD). Double-stranded RNA corresponding to a gfp fragment showed no toxicity towards CPB adults on the day of the final assessment (day 19). This experiment clearly showed that the survival of CPB adults was severely reduced only after a few days of exposure to dsRNA when delivered orally. For example, for target 10, on day 5, 5 out of 10 adults were moribund (sick and slow moving); on day 6, 4 out of 10 adults were dead with three of the survivors moribund; on day 9 all adults were observed dead.
As a consequence of this experiment, the application of target double-stranded RNAs against insect pests may be broadened to include the two life stages of an insect pest (i.e. larvae and adults) which could cause extensive crop damage, as is the case with the Colorado potato beetle.
I. Laboratory Trials to Test Transgenic Potato Plants Against Larvae of the Colorado Potato Beetle, Leptinotarsa decemlineata
The example provided below is an exemplification of the finding that transgenic potato plants expressing CPB-gene-specific hairpin RNAs adversely affected Colorado potato beetles.

Potato Transformation

Stably transformed potato plants were obtained using an adapted protocol received through Julie Gilbert at the NSF Potato Genome Project (http://www.potatogenome.org/nsf5). Stem internode explants of potato ‘Line V’ (obtained from the Laboratory of Plant Breeding at PRI Wageningen, the Netherlands) which was derived from the susceptible diploid Solanum tuberosum 6487-9 were used as starting material for transformation.
In vitro derived explants were inoculated with Agrobacterium tumifaciens C58C₁Rif^Rcontaining the hairpin constructs. After three days co-cultivation the explants were put onto a selective medium containing 100 mg/l Kanamycin and 300 mg/l Timentin. After 6 weeks post-transformation the first putative shoots were removed and rooted on selective medium. Shoots originating from different explants were treated as independent events, shoots originating from the same callus were termed ‘siblings’ until their clonal status can be verified by Southerns, and nodal cuttings of a shoot were referred to as ‘clones’.
The transgenic status of the rooting shoots was checked either by GFP fluorescence or by plus/minus PCR for the target sequence. Positive shoots were then clonally propagated in tissue culture to ensure enough replicates were available for the Colorado potato beetle assay with the first plants being available to test fourteen weeks post transformation.

Bioassay

Transgenic potato plants were grown to the 8-12 unfolded leaf stage in a plant growth room chamber with the following conditions: 23±2° C., 60% relative humidity, 16:8 hour light:dark photoperiod. The plants were caged by placing a 500 ml bottle upside down over the plant with the neck of the bottle firmly placed in the soil in a pot and base cut open and covered with a fine nylon mesh to permit aeration, reduce condensation inside and prevent larval escape.
In this bioassay, seven neonate CPB larvae were placed on the foliage of each transgenic potato plant. Six transgenic potato siblings per transformation event (i.e. plants derived from one callus) of the hairpin construct LD002 (comprising SEQ ID NO 240) (labeled as pGBNB001/28A to F) and empty vector (labeled as pK7GWIWG2D(II)/11A to F), and two wild type plants were tested. Temperature, humidity and lighting conditions were the same as described above. At day 7 (7 days after the start of the bioassay), the number of survivors were counted and the average weight of larval survivors from each plant recorded. Data was analysed using the Spotfire® DecisionSite® 9.0 software (Version 17.1.779) from Spotfire Inc.
In this experiment, all larvae of the Colorado potato beetle on two sibling plants (labeled as pGBNB001/28A and pGBNB001/28F), harbouring hairpin construct LD002, generated from a single transformation event, were dead on day 7 (FIG. 7-LD). Feeding damage by CPB larvae on these two plants was very low when compared to the empty vector transgenic plants or wild type line V plants.

Example 4

Phaedon cochleariae (Mustard Leaf Beetle)

A. Cloning of a Partial Sequence of the Phaedon cochleariae (Mustard Leaf Beetle) PC001, PC003, PC005, PC010, PC014, PC016 and PC027 genes via family PCR High quality, intact RNA was isolated from the third larval stage of Phaedon cochleariae (mustard leaf beetle; source: Dr. Caroline Muller, Julius-von-Sachs-Institute for Biosciences, Chemical Ecology Group, University of Wuerzburg, Julius-von-Sachs-Platz 3, D-97082 Wuerzburg, Germany) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase (Cat. No. 1700, Promega) treatment following the manufacturer's instructions. cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the PC001, PC003, PC005, PC010, PC014, PC016 and PC027 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manafacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-PC. These primers were used in respective PCR reactions with the following conditions: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR4/TOPO vector (Cat. No. K4530-20, Invitrogen) and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-PC and are referred to as the partial sequences.
The corresponding partial amino acid sequence are represented by the respective SEQ ID NOs as given in Table 3-PC. Table 3-PC provides amino acid sequences of cDNA clones, and the start of the reading frame is indicated in brackets.
B. dsRNA Production of the Phaedon cochleariae Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-PC. Table 8-PC provides details for preparing ds RNA fragments of Phaedon cochleariae target sequences, including primer sequences.
The conditions in the PCR reactions were as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 60° C. and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 50° C. and 1 minute at 72° C. followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-PC. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-PC.
C. Recombination of the Phaedon cochleariae (Mustard Leaf Beetle) Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, were cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs were generated using the LR recombination reaction between an attL-containing entry clone (see Example 4A) and an attR-containing destination vector (=pK7GWIWG2D(1)). The plant vector pK7GWIWG2D(II) was obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction was performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments resulted in a hairpin construct for each of the PC001, PC010, PC014, PC016 and PC027 genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example 4B): for PC001, a double digest with BsoBI & PvuI; for PC010, a double digest with PvuI & PvuII; for PC014, a triple digest with HincII, PvuI & XhoI; for PC016, a single digest with ApaLI; for PC027, a double digest with AvaI & DrdI. The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) was purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) was added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix was transformed into Top 10 chemically competent cells. Positive clones were selected by restriction digest analyses. The complete sequence of the hairpin construct for:
PC001 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 508;
PC010 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 509;
PC014 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 510;
PC016 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 511;
PC027 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 512;
Table 9-PC provides sequences for each hairpin construct.
D. Laboratory Trials to Test dsRNA Targets, Using Oilseed Rape Leaf Discs for Activity Against Phaedon cochleariae Larvae
The example provided below is an exemplification of the finding that the mustard leaf beetle (MLB) larvae are susceptible to orally ingested dsRNA corresponding to own target genes.
To test the different double-stranded RNA samples against MLB larvae, a leaf disc assay was employed using oilseed rape (Brassica napus variety SW Oban; source: Nick Balaam, Sw Seed Ltd., 49 North Road, Abington, Cambridge, CB1 6AS, UK) leaf material as food source. The insect cultures were maintained on the same variety of oilseed rape in the insect chamber at 25±2° C. and 60±5% relative humidity with a photoperiod of 16 h light/8 h dark. Discs of approximately 1.1 cm in diameter (or 0.95 cm²) were cut out off leaves of 4- to 6-week old rape plants using a suitably-sized cork borer. Double-stranded RNA samples were diluted to 0.1 μg/μl in Milli-Q water containing 0.05% Triton X-100. Treated leaf discs were prepared by applying 25 μl of the diluted solution of target PC001, PC003, PC005, PC010, PC014, PC016, PC027 dsRNA and control gfp dsRNA or 0.05% Triton X-100 on the adaxial leaf surface. The leaf discs were left to dry and placed individually in each of the 24 wells of a 24-well multiplate containing 1 ml of gellified 2% agar which helps to prevent the leaf disc from drying out. Two neonate MLB larvae were placed into each well of the plate, which was then covered with a multiwell plastic lid. The plate (one treatment containing 48 insects) was divided into 4 replicates of 12 insects per replicate (each row). The plate containing the insects and leaf discs were kept in an insect chamber at 25±2° C. and 60±5% relative humidity with a photoperiod of 16 h light/8 h dark. The insects were fed leaf discs for 2 days after which they were transferred to a new plate containing freshly treated leaf discs. Thereafter, 4 days after the start of the bioassay, the insects from each replicate were collected and transferred to a Petri dish containing untreated fresh oilseed rape leaves. Larval mortality and average weight were recorded at days 2, 4 7, 9 and 11.
P. cochleariae larvae fed on intact naked target dsRNA-treated oilseed rape leaves resulted in significant increases in larval mortalities for all targets tested, as indicated in FIG. 1( a). Tested double-stranded RNA for target PC010 led to 100% larval mortality at day 9 and for target PC027 at day 11. For all other targets, significantly high mortality values were reached at day 11 when compared to control gfp dsRNA, 0.05% Trition X-100 alone or untreated leaf only: (average value in percentage confidence interval with alpha 0.05) PC001 (94.4±8.2); PC003 (86.1±4.1); PC005 (83.3±7.8); PC014 (63.9±20.6); PC016 (75.0±16.8); gfp dsRNA (11.1±8.2); 0.05% Triton X-100 (19.4±10.5); leaf only (8.3±10.5).
Larval survivors were assessed based on their average weight. For all targets tested, the mustard leaf beetle larvae had significantly reduced average weights after day 4 of the bioassay; insects fed control gfp dsRNA or 0.05% Triton X-100 alone developed normally, as for the larvae on leaf only (FIG. 1( b)-PC).
E. Laboratory Trials to Screen dsRNAs at Different Concentrations Using Oilseed Rape Leaf Discs for Activity Against Phaedon cochleariae Larvae
Twenty-five μl of a solution of dsRNA from target PC010 or PC027 at serial ten-fold concentrations from 0.1 μg/μl down to 0.1 ng/μl was applied topically onto the oilseed rape leaf disc, as described in Example 4D above. As a negative control, 0.05% Triton X-100 only was administered to the leaf disc. Per treatment, twenty-four mustard leaf beetle neonate larvae, with two insects per well, were tested. The plates were stored in the insect rearing chamber at 25±2° C., 60±5% relative humidity, with a 16:8 hours light:dark photoperiod. At day 2, the larvae were transferred on to a new plate containing fresh dsRNA-treated leaf discs. At day 4 for target PC010 and day 5 for target PC027, insects from each replicate were transferred to a Petri dish containing abundant untreated leaf material. The beetles were assessed as live or dead on days 2, 4, 7, 8, 9, and 11 for target PC010, and 2, 5, 8, 9 and 12 for target PC027.
Feeding oilseed rape leaf discs containing intact naked dsRNAs of the two different targets, PC010 and PC027, to P. cochleariae larvae resulted in high mortalities at concentrations down to as low as 1 ng dsRNA/μl solution, as shown in FIGS. 2 (a) and (b). Average mortality values in percentage±confidence interval with alpha 0.05 for different concentrations of dsRNA for target PC010 at day 11, 0 μg/μl: 8.3±9.4; 0.1 μg/μl: 100; 0.01 μg/μl: 79.2±20.6; 0.001 μg/μl: 58.3±9.4; 0.0001 μg/μl: 12.5±15.6; and for target PC027 at day 12, 0 μg/μl: 8.3±9.4; 0.1 μg/μl: 95.8±8.2; 0.01 μg/μl: 95.8±8.2; 0.001 μg/μl: 83.3±13.3; 0.0001 μg/μl: 12.5±8.2.
F. Laboratory Trials of Myzus periscae (Green Peach Aphid) Infestation on Transgenic Arabidopsis thaliana Plants

Generation of Transgenic Plants

Arabidopsis thaliana plants were transformed using the floral dip method (Clough and Bent (1998) Plant Journal 16:735-743). Aerial parts of the plants were incubated for a few seconds in a solution containing 5% sucrose, resuspended Agrobacterium tumefaciens strain C58C1 Rif cells from an overnight culture and 0.03% of the surfactant Silwet L-77. After inoculation, plants were covered for 16 hours with a transparent plastic to maintain humidity. To increase the transformation efficiency, the procedure was repeated after one week. Watering was stopped as seeds matured and dry seeds were harvested and cold-treated for two days. After sterilization, seeds were plated on a kanamycin-containing growth medium for selection of transformed plants.
The selected plants are transferred to soil for optimal T2 seed production.

Bioassay

Transgenic Arabidopsis thaliana plants are selected by allowing the segregating T2 seeds to germinate on appropriate selection medium. When the roots of these transgenics are well-established they are then transferred to fresh artificial growth medium or soil and allowed to grow under optimal conditions. Whole transgenic plants are tested against nymphs of the green peach aphid (Myzus persicae) to show (1) a significant resistance to plant damage by the feeding nymph, (2) increased nymphal mortality, and/or (3) decreased weight of nymphal survivors (or any other aberrant insect development).

Example 5

Epilachna varivetis (Mexican Bean Beetle)

A. Cloning Epilachna varivetis Partial Gene Sequences
High quality, intact RNA was isolated from 4 different larval stages of Epilachna varivetis (Mexican bean beetle; source: Thomas Dorsey, Supervising Entomologist, New Jersey Department of Agriculture, Division of Plant Industry, Bureau of Biological Pest Control, Phillip Alampi Beneficial Insect Laboratory, PO Box 330, Trenton, N.J. 08625-0330, USA) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manafacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the EV005, EV009, EV010, EV015 and EV016 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manufacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-EV, which displays Epilachna varivetis target genes including primer sequences and cDNA sequences obtained. These primers were used in respective PCR reactions with the following conditions: for EV005 and EV009, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 50° C. and 1 minute 30 seconds at 72° C., followed by 7 minutes at 72° C.; for EV014, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 53° C. and 1 minute at 72° C., followed by 7 minutes at 72° C.; for EV010 and EV016, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute 40 seconds at 72° C., followed by 7 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR4/TOPO vector (Cat. No. K4530-20, Invitrogen), and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-EV and are referred to as the partial sequences. The corresponding partial amino acid sequences are represented by the respective SEQ ID NOs as given in Table 3-EV, where the start of the reading frame is indicated in brackets.
B. dsRNA Production of the Epilachna varivetis Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-EV.
The conditions in the PCR reactions were as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 60° C. and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 50° C. and 1 minute at 72° C. followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-EV. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-EV.
C. Recombination of the Epilachna varivetis Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, are cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs are generated using the LR recombination reaction between an attL-containing entry clone (see Example 5A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) is obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction is performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments result in a hairpin construct for each of the target genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) is purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) is added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix is transformed into Top 10 chemically competent cells. Positive clones are selected by restriction digest analyses.
D. Laboratory Trials to Test dsRNA Targets Using Bean Leaf Discs for Activity Against Epilachna varivetis Larvae
The example provided below is an exemplification of the finding that the Mexican bean beetle (MBB) larvae are susceptible to orally ingested dsRNA corresponding to own target genes.
To test the different double-stranded RNA samples against MBB larvae, a leaf disc assay was employed using snap bean (Phaseolus vulgaris variety Montano; source: Aveve NV, Belgium) leaf material as food source. The same variety of beans was used to maintain insect cultures in the insect chamber at 25±2° C. and 60±5% relative humidity with a photoperiod of 16 h light/8 h dark. Discs of approximately 1.1 cm in diameter (or 0.95 cm²) were cut out off leaves of 1- to 2-week old bean plants using a suitably-sized cork borer. Double-stranded RNA samples were diluted to 1 μg/μl in Milli-Q water containing 0.05% Triton X-100. Treated leaf discs were prepared by applying 25 μl of the diluted solution of target Ev005, Ev010, Ev015, Ev016 dsRNA and control gfp dsRNA or 0.05% Triton X-100 on the adaxial leaf surface. The leaf discs were left to dry and placed individually in each of the 24 wells of a 24-well multiplate containing 1 ml of gellified 2% agar which helps to prevent the leaf disc from drying out. A single neonate MBB larva was placed into each well of a plate, which was then covered with a multiwell plastic lid. The plate was divided into 3 replicates of 8 insects per replicate (row). The plate containing the insects and leaf discs were kept in an insect chamber at 25±2° C. and 60±5% relative humidity with a photoperiod of 16 h light/8 h dark. The insects were fed on the leaf discs for 2 days after which the insects were transferred to a new plate containing freshly treated leaf discs. Thereafter, 4 days after the start of the bioassay, the insects were transferred to a petriplate containing untreated fresh bean leaves every day until day 10. Insect mortality was recorded at day 2 and every other day thereafter.
Feeding snap bean leaves containing surface-applied intact naked target dsRNAs to E. varivestis larvae resulted in significant increases in larval mortalities, as indicated in FIG. 1. Tested double-stranded RNAs of targets Ev010, Ev005, & Ev016 led to 100% mortality after 8 days, whereas dsRNA of target Ev005 took 10 days to kill all larvae. The majority of the insects fed on treated leaf discs containing control gfp dsRNA or only the surfactant Triton X-100 were sustained throughout the bioassay (FIG. 1-EV).
E. Laboratory Trials to Test dsRNA Targets Using Bean Leaf Discs for Activity Against Epilachna varivestis Adults
The example provided below is an exemplification of the finding that the Mexican bean beetle adults are susceptible to orally ingested dsRNA corresponding to own target genes.
In a similar bioassay set-up as for Mexican bean beetle larvae, adult MBBs were tested against double-stranded RNAs topically-applied to bean leaf discs. Test dsRNA from each target Ev010, Ev015 and Ev016 was diluted in 0.05% Triton X-100 to a final concentration of 0.1 μg/μl. Bean leaf discs were treated by topical application of 30 μl of the test solution onto each disc. The discs were allowed to dry completely before placing each on a slice of gellified 2% agar in each well of a 24-well multiwell plate. Three-day-old adults were collected from the culture cages and fed nothing for 7-8 hours prior to placing one adult to each well of the bioassay plate (thus 24 adults per treatment). The plates were kept in the insect rearing chamber (under the same conditions as for MBB larvae for 24 hours) after which the adults were transferred to a new plate containing fresh dsRNA-treated leaf discs. After a further 24 hours, the adults from each treatment were collected and placed in a plastic box with dimensions 30 cm×15 cm×10 cm containing two potted and untreated 3-week-old bean plants. Insect mortality was assessed from day 4 until day 11.
All three target dsRNAs (Ev010, Ev015 and Ev016) ingested by adults of Epilachna varivestis resulted in significant increases in mortality from day 4 (4 days post bioassay start), as shown in FIG. 2-EV(a). From day 5, dramatic changes in feeding patterns were observed between insects fed initially with target-dsRNA-treated bean leaf discs and those that were fed discs containing control gfp dsRNA or surfactant Triton X-100. Reductions in foliar damage by MBB adults of untreated bean plants were clearly visible for all three targets when compared to gfp dsRNA and surfactant only controls, albeit at varying levels; insects fed target 15 caused the least damage to bean foliage (FIG. 2-EV(b)).

Example 6

Anthonomus grandis (Cotton Boll Weevil)

A. Cloning Anthonomus grandis Partial Sequences
High quality, intact RNA was isolated from the 3 instars of Anthonomus grandis (cotton boll weevil; source: Dr. Gary Benzon, Benzon Research Inc., 7 Kuhn Drive, Carlisle, Pa. 17013, USA) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manafacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the AG001, AG005, AG010, AG014 and AG016 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manafacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-AG. These primers were used in respective PCR reactions with the following conditions: for AG001, AG005 and AG016, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 50° C. and 1 minute and 30 seconds at 72° C., followed by 7 minutes at 72° C.; for AG010, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 2 minutes and 30 seconds at 72° C., followed by 7 minutes at 72° C.; for AG014, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 7 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/TOPO vector (Cat. No. K2500-20, Invitrogen) and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-AG and are referred to as the partial sequences. The corresponding partial amino acid sequence are represented by the respective SEQ ID NOs as given in Table 3-AG.
B. dsRNA Production of the Anthonomus grandis (Cotton Boll Weevil) genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-AG. A touchdown PCR was performed as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 60° C. with a decrease in temperature of 0.5° C. per cycle and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 50° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-AG. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-AG.
C. Recombination of Anthonomus grandis Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, are cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs are generated using the LR recombination reaction between an attL-containing entry clone (see Example 6A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) is obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction is performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments result in a hairpin construct for each of the target genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example 6B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) is purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) is added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix is transformed into Top 10 chemically competent cells. Positive clones are selected by restriction digest analyses.
D. Laboratory Trials to Test Escherichia coli Expressing dsRNA Targets Against Anthonomus grandis

Plant-Based Bioassays

Whole plants are sprayed with suspensions of chemically induced bacteria expressing dsRNA prior to feeding the plants to CBW. The are grown from in a plant growth room chamber. The plants are caged by placing a 500 ml plastic bottle upside down over the plant with the neck of the bottle firmly placed in the soil in a pot and the base cut open and covered with a fine nylon mesh to permit aeration, reduce condensation inside and prevent insect escape. CBW are placed on each treated plant in the cage. Plants are treated with a suspension of E. coli AB301-105(DE3) harboring the pGXXX0XX plasmids or pGN29 plasmid. Different quantities of bacteria are applied to the plants: for instance 66, 22, and 7 units, where one unit is defined as 109 bacterial cells in 1 ml of a bacterial suspension at optical density value of 1 at 600 nm wavelength. In each case, a total volume of between 1 and 10 ml s sprayed on the plant with the aid of a vaporizer. One plant is used per treatment in this trial. The number of survivors are counted and the weight of each survivor recorded.
Spraying plants with a suspension of E. coli bacterial strain AB301-105(DE3) expressing target dsRNA from pGXXX0XX lead to a dramatic increase in insect mortality when compared to pGN29 control. These experiments show that double-stranded RNA corresponding to an insect gene target sequence produced in either wild-type or RNaseIII-deficient bacterial expression systems is toxic towards the insect in terms of substantial increases in insect mortality and growth/development delay for larval survivors. It is also clear from these experiments that an exemplification is provided for the effective protection of plants/crops from insect damage by the use of a spray of a formulation consisting of bacteria expressing double-stranded RNA corresponding to an insect gene target.

Example 7

Tribolium castaneum (Red Flour Beetle)

A. Cloning Tribolium castaneum Partial Sequences
High quality, intact RNA was isolated from all the different insect stages of Tribolium castaneum (red flour beetle; source: Dr. Lara Senior, Insect Investigations Ltd., Capital Business Park, Wentloog, Cardiff, CF3 2PX, Wales, UK) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manafacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the TC001, TC002, TC010, TC014 and TC015 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manafacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-TC. These primers were used in respective PCR reactions with the following conditions: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 50° C. and 1 minute and 30 seconds at 72° C., followed by 7 minutes at 72° C. (TC001, TC014, TC015); 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 2 minutes and 30 seconds at 72° C., followed by 7 minutes at 72° C. (TC010); 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 53° C. and 1 minute at 72° C., followed by 7 minutes at 72° C. (TC002). The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/TOPO vector (Cat. No. K2500-20, Invitrogen), and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-TC and are referred to as the partial sequences. The corresponding partial amino acid sequences are represented by the respective SEQ ID NOs as given in Table 3-TC.
B. dsRNA Production of the Tribolium castaneum Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-TC. The conditions in the PCR reactions were as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 60° C. (−0.5° C./cycle) and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 50° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-TC. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-TC.
C. Recombination of Tribolium castaneum Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, are cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs are generated using the LR recombination reaction between an attL-containing entry clone (see Example 7A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) is obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction is performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments result in a hairpin construct for each of the target genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example 7B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) is purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) is added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix is transformed into Top 10 chemically competent cells. Positive clones are selected by restriction digest analyses.
D. Laboratory Trials to Test dsRNA Targets, Using Artificial Diet for Activity Against Tribolium castaneum Larvae
The example provided below is an exemplification of the finding that the red flour beetle (RFB) larvae are susceptible to orally ingested dsRNA corresponding to own target genes.
Red flour beetles, Tribolium castaneum, were maintained at Insect Investigations Ltd. (origin: Imperial College of Science, Technology and Medicine, Silwood Park, Berkshire, UK). Insects were cultured according to company SOP/251/01. Briefly, the beetles were housed in plastic jars or tanks. These have an open top to allow ventilation. A piece of netting was fitted over the top and secured with an elastic band to prevent escape. The larval rearing medium (flour) was placed in the container where the beetles can breed. The stored product beetle colonies were maintained in a controlled temperature room at 25±3° C. with a 16:8 hour light:dark cycle.
Double-stranded RNA from target TC014 (with sequence corresponding to SEQ ID NO −799) was incorporated into a mixture of flour and milk powder (wholemeal flour: powdered milk in the ratio 4:1) and left to dry overnight. Each replicate was prepared separately: 100 μl of a 10 μg/μl dsRNA solution (1 mg dsRNA) was added to 0.1 g flour/milk mixture. The dried mixture was ground to a fine powder. Insects were maintained within Petri dishes (55 mm diameter), lined with a double layer of filter paper. The treated diet was placed between the two filter paper layers. Ten first instar, mixed sex larvae were placed in each dish (replicate). Four replicates were performed for each treatment. Control was Milli-Q water. Assessments (number of survivors) were made on a regular basis. During the trial, the test conditions were 25-33° C. and 20-25% relative humidity, with a 12:12 hour light:dark photoperiod.
Survival of larvae of T. castaneum over time on artificial diet treated with target TC014 dsRNA was significantly reduced when compared to diet only control, as shown in FIG. 1-TC.

Example 8

Myzus persicae (Preen Peach Aphid)

A Cloning Myzus persicae partial sequences High quality, intact RNA was isolated from nymphs of Myzus persicae (green peach aphid; source: Dr. Rachel Down, Insect & Pathogen Interactions, Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK) using TRizol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manafacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the MP001, MP002, MP010, MP016 and MP027 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manafacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-MP. These primers were used in respective PCR reactions with the following conditions: for MP001, MP002 and MP016, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 50° C. and 1 minute 30 seconds at 72° C., followed by 7 minutes at 72° C.; for MP027, a touchdown program was used: 10 minutes at 95° C., followed by 10 cycles of 30 seconds at 95° C., 40 seconds at 60° C. with a decrease in temperature of 1° C. per cycle and 1 minute 10 seconds at 72° C., followed by 30 cycles of 30 seconds at 95° C., 40 seconds at 50° C. and 1 minute 10 seconds at 72° C., followed by 7 minutes at 72° C.; for MP010, 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 3 minutes at 72° C., followed by 7 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/TOPO vector (Cat. No. K2500-20, Invitrogen), and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-MP and are referred to as the partial sequences. The corresponding partial amino acid sequences are represented by the respective SEQ ID NOs as given in Table 3-MP.
B. dsRNA Production of Myzus persicae Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-MP. A touchdown PCR was performed as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 55° C. (for MP001, MP002, MP016, MP027 and gfp) or 30 seconds at 50° C. (for MP010) with a decrease in temperature of 0.5° C. per cycle and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 45° C. and 1 minute at 72° C. followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-MP. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-MP.
C. Recombination of Myzus persicae Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, were cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs were generated using the LR recombination reaction between an attL-containing entry clone (see Example 8A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) was obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction was performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments resulted in a hairpin construct for each of the MP001, MP002, MP010, MP016 and MP026 genes, having the promoter-sense-intron-CmR-intron-antisense orientation and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
A digest with restriction enzyme Alw441 was done for all the targets cloned into pCR8/GW/topo (see Example 8B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) was purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) was added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix was transformed into Top 10 chemically competent cells. Positive clones were selected by restriction digest analysis. The complete sequence of the hairpin construct for:
MP001 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 1066;
MP002 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 1067;
MP010 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 1068;
MP016 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 1069;
MP027 (sense-intron-CmR-intron-antisense) is represented in SEQ ID NO 1070.
Table 9-MP provides complete sequences for each hairpin construct.
D. Laboratory Trials to Test dsRNA Targets Using Liquid Artificial Diet for Activity Against Myzus persicae
Liquid artificial diet for the green peach aphid, Myzus persicae, was prepared based on the diet suitable for pea aphids (Acyrthosiphon pisum), as described by Febvay et al. (1988) [Influence of the amino acid balance on the improvement of an artificial diet for a biotype of Acyrthosiphon pisum (Homoptera: Aphididae). Can. J. Zool. 66: 2449-2453], but with some modifications. The amino acids component of the diet was prepared as follows: in mg/100 ml, alanine 178.71, beta-alanine 6.22, arginine 244.9, asparagine 298.55, aspartic acid 88.25, cysteine 29.59, glutamic acid 149.36, glutamine 445.61, glycine 166.56, histidine 136.02, isoleucine 164.75, leucine 231.56, lysine hydrochloride 351.09, methionine 72.35, ornithine (HCl) 9.41, phenylalanine 293, proline 129.33, serine 124.28, threonine 127.16, tryptophane 42.75, tyrosine 38.63, L-valine 190.85. The amino acids were dissolved in 30 ml Milli-Q H₂O except for tyrosine which was first dissolved in a few drops of 1 M HCl before adding to the amino acid mix. The vitamin mix component of the diet was prepared as a 5× concentrate stock as follows: in mg/L, amino benzoic acid 100, ascorbic acid 1000, biotin 1, calcium panthothenate 50, choline chloride 500, folic acid 10, myoinositol 420, nicotinic acid 100, pyridoxine hydrochloride 25, riboflavin 5, thiamine hydrochloride 25. The riboflavin was dissolved in 1 ml H₂O at 50° C. and then added to the vitamin mix stock. The vitamin mix was aliquoted in 20 ml per aliquot and stored at −20° C. One aliquot of vitamin mix was added to the amino acid solution. Sucrose and MgSO₄.7H₂O was added with the following amounts to the mix: 20 g and 242 mg, respectively. Trace metal stock solution was prepared as follows: in mg/100 ml, CuSO₄.5H₂O 4.7, FeCl₃.6H₂O 44.5, MnCl₂.4H₂O 6.5, NaCl 25.4, ZnCl₂8.3. Ten ml of the trace metal solution and 250 mg KH₂PO₄was added to the diet and Milli-Q water was added to a final liquid diet volume of 100 ml. The pH of the diet was adjusted to 7 with 1 M KOH solution. The liquid diet was filter-sterilised through an 0.22 μm filter disc (Millipore).
Green peach aphids (Myzus persicae; source: Dr. Rachel Down, Insect & Pathogen Interactions, Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK) were reared on 4- to 6-week-old oilseed rape (Brassica napus variety SW Oban; source: Nick Balaam, Sw Seed Ltd., 49 North Road, Abington, Cambridge, CB1 6AS, UK) in aluminium-framed cages containing 70 μm mesh in a controlled environment chamber with the following conditions: 23±2° C. and 60±5% relative humidity, with a 16:8 hours light:dark photoperiod.
One day prior to the start of the bioassay, adults were collected from the rearing cages and placed on fresh detached oilseed rape leaves in a Petri dish and left overnight in the insect chamber. The following day, first-instar nymphs were picked and transferred to feeding chambers. A feeding chamber comprised of 10 first instar nymphs placed in a small Petri dish (with diameter 3 cm) covered with a single layer of thinly stretched parafilm M onto which 50 μl of diet was added.
The chamber was sealed with a second layer of parafilm and incubated under the same conditions as the adult cultures. Diet with dsRNA was refreshed every other day and the insects' survival assessed on day 8 i.e. 8^thday post bioassay start. Per treatment, 5 bioassay feeding chambers (replicates) were set up simultaneously. Test and control (gfp) dsRNA solutions were incorporated into the diet to a final concentration of 2 μg/μl. The feeding chambers were kept at 23±2° C. and 60±5% relative humidity, with a 16:8 hours light:dark photoperiod. A Mann-Whitney test was determined by GraphPad Prism version 4 to establish whether the medians do differ significantly between target 27 (MP027) and gfp dsRNA.
In the bioassay, feeding liquid artificial diet supplemented with intact naked dsRNA from target 27 (SEQ ID NO 1061) to nymphs of Myzus persicae using a feeding chamber, resulted in a significant increase in mortality, as shown in FIG. 1. Average percentage survivors for target 27, gfp dsRNA and diet only treatment were 2, 34 and 82, respectively. Comparison of target 027 with gfp dsRNA groups using the Mann-Whitney test resulted in an one-tailed P-value of 0.004 which indicates that the median of target 027 is significantly different (P<0.05) from the expected larger median of gfp dsRNA. The green peach aphids on the liquid diet with incorporated target 27 dsRNA were noticeably smaller than those that were fed on diet only or with gfp dsRNA control (data not presented).
E. Laboratory Trials of Myzus periscae (Green Peach Aphid) Infestation on Transgenic Arabidopsis thaliana Plants

Generation of Transgenic Plants

Bioassay

Example 9

Nilaparvata lugens (Brown Plant Hopper)

A. Cloning Nilaparvata lugens Partial Sequences
From high quality total RNA of Nilaparvata lugens (source: Dr. J. A. Gatehouse, Dept. Biological Sciences, Durham University, UK) cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's protocol.
To isolate cDNA sequences comprising a portion of the Nilaparvata lugens NL001, NL002, NL003, NL004, NL005, NL006, NL007, NL008, NL009, NL010, NL011, NL012, NL013, NL014, NL015, NL016, NL018, NL019, NL021, NL022, and NL027 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat No. N8080240; Applied Biosystems) following the manufacturer's protocol.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-NL. These primers were used in respective PCR reactions with the following conditions: for NL001: 5 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.: for NL002: 3 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL003: 3 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 61° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL004: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 51° C. and 1 minute at 72° C.; for NL005: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL006: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 3 minute 30 seconds at 72° C., followed by 10 minutes at 72° C.; for NL007: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute 15 seconds at 72° C., followed by 10 minutes at 72° C.; for NL008 & NL014: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 53° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL009, NL011, NL012 & NL019: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL010: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 2 minute 30 seconds at 72° C., followed by 10 minutes at 72° C.; for NL013: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute 10 seconds at 72° C., followed by 10 minutes at 72° C.; for NL015 & NL016: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute 40 seconds at 72° C., followed by 10 minutes at 72° C.; for NL018: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute 35 seconds at 72° C., followed by 10 minutes at 72° C.; for NL021, NL022 & NL027: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 1 minute 45 seconds at 72° C., followed by 10 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/topo vector (Cat. No. K2500 20, Invitrogen), and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-NL and are referred to as the partial sequences. The corresponding partial amino acid sequences are represented by the respective SEQ ID NOs as given in Table 3-NL.
B. Cloning of a Partial Sequence of the Nilaparvata lugens NL023 Gene Via EST Sequence
From high quality total RNA of Nilaparvata lugens (source: Dr. J. A. Gatehouse, Dept. Biological Sciences, Durham University, UK) cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's protocol.
A partial cDNA sequence, NL023, was amplified from Nilaparvata lugens cDNA which corresponded to a Nilaparvata lugens EST sequence in the public database Genbank with accession number CAH65679.2. To isolate cDNA sequences comprising a portion of the NL023 gene, a series of PCR reactions with EST based specific primers were performed using PerfectShot™ ExTaq (Cat No. RR005A, Takara Bio Inc.) following the manafacturer's protocol.
For NL023, the specific primers oGBKW002 and oGBKW003 (represented herein as SEQ ID NO 1157 and SEQ ID NO 1158, respectively) were used in two independent PCR reactions with the following conditions: 3 minutes at 95° C., followed by 30 cycles of 30 seconds at 95° C., 30 seconds at 56° C. and 2 minutes at 72° C., followed by 10 minutes at 72° C. The resulting PCR products were analyzed on agarose gel, purified (QIAquick® Gel Extraction Kit; Cat. No. 28706, Qiagen), cloned into the pCR4-TOPO vector (Cat No. K4575-40, Invitrogen) and sequenced. The consensus sequence resulting from the sequencing of both PCR products is herein represented by SEQ ID NO 1111 and is referred to as the partial sequence of the NL023 gene. The corresponding partial amino acid sequence is herein represented as SEQ ID NO 1112.
C. dsRNA Production of Nilaparvata lugens Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-NL. The conditions in the PCR reactions were as follows: for NL001 & NL002: 4 minutes at 94° C., followed by 35 cycles of 30 seconds at 94° C., 30 seconds at 60° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL003: 4 minutes at 94° C., followed by 35 cycles of 30 seconds at 94° C., 30 seconds at 66° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL004, NL006, NL008, NL009, NL010 & NL019: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 54° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL005 & NL016: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 57° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL007 & NL014: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 51° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL011, NL012 & NL022: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 53° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL013, NL015, NL018 & NL021: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C.; for NL023 & NL027: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 52° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-NL. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen). The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions, but with the following modification: RNA peppet is washed twice in 70% ethanol. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-NL.
The template DNA used for the PCR reactions with T7 primers on the green fluorescent protein (gfp) control was the plasmid pPD96.12 (the Fire Lab, http://genome-www.stanford.edu/group/fire/), which contains the wild-type gfp coding sequence interspersed by 3 synthetic introns. Double-stranded RNA was synthesized using the commercially available kit T7 RiboMAX™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter. For gfp, the sense T7 template was generated using the specific T7 FW primer oGAU183 and the specific RV primer oGAU182 (represented herein as SEQ ID NO 236 and SEQ ID NO 237, respectively) in a PCR reaction with the following conditions: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using the specific FW primer oGAU181 and the specific T7 RV primer oGAU184 (represented herein as SEQ ID NO 238 and SEQ ID NO 239, respectively) in a PCR reaction with the same conditions as described above. The resulting PCR products were analyzed on agarose gel and purified (QIAquick® PCR Purification Kit; Cat. No. 28106, Qiagen). The generated T7 FW and RV templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by precipitation with sodium acetate and isopropanol, following the manufacturer's protocol, but with the following modification: RNA peppet is washed twice in 70% ethanol. The sense strands of the resulting dsRNA is herein represented by SEQ ID NO 235.
D. Laboratory Trials to Screen dsRNA Targets Using Liquid Artificial Diet for Activity Against Nilaparvata lugens
Liquid artificial diet (MMD-1) for the rice brown planthopper, Nilaparvata lugens, was prepared as described by Koyama (1988) [Artificial rearing and nutritional physiology of the planthoppers and leafhoppers (Homoptera: Delphacidae and Deltocephalidae) on a holidic diet. JARQ 22: 20-27], but with a modification in final concentration of diet component sucrose: 14.4% (weight over volume) was used. Diet components were prepared as separate concentrates: 10× mineral stock (stored at 4° C.), 2× amino acid stock (stored at −20° C.) and 10× vitamin stock (stored at −20° C.). The stock components were mixed immediately prior to the start of a bioassay to 4/3× concentration to allow dilution with the test dsRNA solution (4× concentration), pH adjusted to 6.5, and filter-sterilised into approximately 500 μl aliquots.
Rice brown planthopper (Nilaparvata lugens) was reared on two-to-three month old rice (Oryza saliva cv Taichung Native 1) plants in a controlled environment chamber: 27±2° C., 80% relative humidity, with a 16:8 hours light:dark photoperiod. A feeding chamber comprised 10 first or second instar nymphs placed in a small petri dish (with diameter 3 cm) covered with a single layer of thinly stretched parafilm M onto which 50 μl of diet was added. The chamber was sealed with a second layer of parafilm and incubated under the same conditions as the adult cultures but with no direct light exposure. Diet with dsRNA was refreshed every other day and the insects' survival assessed daily. Per treatment, 5 bioassay feeding chambers (replicates) were set up simultaneously. Test and control (gfp) dsRNA solutions were incorporated into the diet to a final concentration of 2 mg/ml. The feeding chambers were kept at 27±2° C., 80% relative humidity, with a 16:8 hours light:dark photoperiod. Insect survival data were analysed using the Kaplan-Meier survival curve model and the survival between groups were compared using the logrank test (Prism version 4.0).
Feeding liquid artificial diet supplemented with intact naked dsRNAs to Nilaparvata lugens in vitro using a feeding chamber resulted in significant increases in nymphal mortalities as shown in four separate bioassays (FIGS. 1( a)-(d)-NL; Tables 10-NL(a)-d)) (Durham University). These results demonstrate that dsRNAs corresponding to different essential BPH genes showed significant toxicity towards the rice brown planthopper.
Effect of gfp dsRNA on BPH survival in these bioassays is not significantly different to survival on diet only
Tables 10-NL(a)-d) show a summary of the survival of Nilaparvata lugens on artificial diet supplemented with 2 mg/ml (final concentration) of the following targets; in Table 10-NL(a): NL002, NL003, NL005, NL010; in Table 10-NL(b): NL009, NL016; in Table 10-NL(c): NL014, NL018; and in Table 10-NL(d): NL013, NL015, NL021. In the survival analysis column, the effect of RNAi is indicated as follows: +=significantly decreased survival compared to gfp dsRNA control (alpha<0.05); -=no significant difference in survival compared to gfp dsRNA control. Survival curves were compared (between diet only and diet supplemented with test dsRNA, gfp dsRNA and test dsRNA, and diet only and gfp dsRNA) using the logrank test.
E. Laboratory Trials to Screen dsRNAs at Different Concentrations Using Artificial Diet for Activity Against Nilaparvata lugens
Fifty μl of liquid artificial diet supplemented with different concentrations of target NL002 dsRNA, namely 1, 0.2, 0.08, and 0.04 mg/ml (final concentration), was applied to the brown planthopper feeding chambers. Diet with dsRNA was refreshed every other day and the insects' survival assessed daily. Per treatment, 5 bioassay feeding chambers (replicates) were set up simultaneously. The feeding chambers were kept at 27±2° C., 80% relative humidity, with a 16:8 hours light:dark photoperiod. Insect survival data were analysed using the Kaplan-Meier survival curve model and the survival between groups were compared using the logrank test (Prism version 4.0).
Feeding liquid artificial diet supplemented with intact naked dsRNAs of target NL002 at different concentrations resulted in significantly higher BPH mortalities at final concentrations of as low as 0.04 mg dsRNA per ml diet when compared with survival on diet only, as shown in FIG. 2-NL and Table 11-NL. Table 11-NL summarizes the survival of Nilaparvata lugens artificial diet feeding trial supplemented with 1, 0.2, 0.08, & 0.04 mg/ml (final concentration) of target NL002. In the survival analysis column the effect of RNAi is indicated as follows: +=significantly decreases survival compared to diet only control (alpha<0.05); −=no significant differences in survival compared to diet only control. Survival curves were compared using the logrank test.

Example 10

Chilo suppressalis (Rice Striped Stem Borer)

A. Cloning of Partial Sequence of the Chilo suppressalis Genes Via Family PCR
High quality, intact RNA was isolated from the 4 different larval stages of Chilo suppressalis (rice striped stem borer) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manafacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ II Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the CS001, CS002, CS003, CS006, CS007, CS009, CS011, CS013, CS014, CS015, CS016 and CS018 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manafacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-CS. These primers were used in respective PCR reactions with the following conditions: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR4/TOPO vector (Cat. No. K2500-20, Invitrogen), and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-CS and are referred to as the partial sequences. The corresponding partial amino acid sequences are represented by the respective SEQ ID NOs as given in Table 3-CS.
B. dsRNA Production of the Chilo suppressalis Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-CS. The conditions in the PCR reactions were as follows: 4 minutes at 95° C., followed by 35 cycles of 30 seconds at 95° C., 30 seconds at 55° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-CS. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-CS.
C. Recombination of the Chilo suppressalis Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, are cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs are generated using the LR recombination reaction between an attL-containing entry clone (see Example 10A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) is obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction is performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments result in a hairpin construct for each of the target genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example 10B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) is purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) is added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix is transformed into Top 10 chemically competent cells. Positive clones are selected by restriction digest analyses.
D. Laboratory Trials to Test dsRNA Targets, Using Artificial Diet for Activity Against Chilo suppressalis Larvae
Rice striped stem borers, Chilo suppressalis, (origin: Syngenta, Stein, Switzerland) were maintained on a modified artificial diet based on that described by Kamano and Sato, 1985 (in: Handbook of Insect Rearing. Volumes I & II. P Singh and R F Moore, eds., Elsevier Science Publishers, Amsterdam and New York, 1985, pp 448). Briefly, a litre diet was made up as follows: 20 g of agar added to 980 ml of Milli-Q water and autoclaved; the agar solution was cooled down to approximately 55° C. and the remaining ingredients were added and mixed thoroughly: 40 g corn flour (Polenta), 20 g cellulose, 30 g sucrose, 30 g casein, 20 g wheat germ (toasted), 8 g Wesson salt mixture, 12 g Vanderzant vitamin mix, 1.8 g sorbic acid, 1.6 g nipagin (methylparaben), 0.3 g aureomycin, 0.4 g cholesterol and 0.6 g L-cysteine. The diet was cooled down to approx. 45° C. and poured into rearing trays or cups. The diet was left to set in a horizontal laminair flow cabin. Rice leaf sections with oviposited eggs were removed from a cage housing adult moths and pinned to the solid diet in the rearing cup or tray. Eggs were left to hatch and neonate larvae were available for bioassays and the maintenance of the insect cultures. During the trials and rearings, the conditions were 28±2° C. and 80±5% relative humidity, with a 16:8 hour light:dark photoperiod.
The same artificial diet is used for the bioassays but in this case the diet is poured equally in 24 multiwell plates, with each well containing 1 ml diet. Once the diet is set, the test formulations are applied to the diet's surface (2 cm²), at the rate of 50 μl of 1 μg/μl dsRNA of target. The dsRNA solutions are left to dry and two first instar moth larvae are placed in each well. After 7 days, the larvae are transferred to fresh treated diet in multiwell plates. At day 14 (i.e. 14 days post bioassay start) the number of live and dead insects is recorded and examined for abnormalities. Twenty-four larvae in total are tested per treatment.
An alternative bioassay is performed in which treated rice leaves are fed to neonate larvae of the rice striped stem borer. Small leaf sections of Indica rice variety Taichung native 1 are dipped in 0.05% Triton X-100 solution containing 1 μg/μl of target dsRNA, left to dry and each section placed in a well of a 24 multiwell plate containing gellified 2% agar. Two neonates are transferred from the rearing tray to each dsRNA treated leaf section (24 larvae per treatment). After 4 and 8 days, the larvae are transferred to fresh treated rice leaf sections. The number of live and dead larvae are assessed on days 4, 8 and 12; any abnormalities are also recorded.

Example 11

Plutella xylostella (Diamondback Moth)

A. Cloning of a Partial Sequence of the Plutella xylostella
High quality, intact RNA was isolated from all the different larval stages of Plutella xylostella (Diamondback moth; source: Dr. Lara Senior, Insect Investigations Ltd., Capital Business Park, Wentloog, Cardiff, CF3 2PX, Wales, UK) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manufacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the PX001, PX009, PX010, PX015, PX016 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manufacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-PX. These primers were used in respective PCR reactions with the following conditions: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 50° C. and 1 minute and 30 seconds at 72° C., followed by 7 minutes at 72° C. (for PX001, PX009, PX015, PX016); 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 54° C. and 2 minute and 30 seconds at 72° C., followed by 7 minutes at 72° C. (for PX010). The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/TOPO vector (Cat. No. K2500-20, Invitrogen) and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-PX and are referred to as the partial sequences. The corresponding partial amino acid sequence are represented by the respective SEQ ID NOs as given in Table 3-PX.
B. dsRNA Production of the Plutella xylostella Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-PX. The conditions in the PCR reactions were as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 60° C. (−0.5° C./cycle) and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 50° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-PX. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-PX.
C. Recombination of the Plutella xylostella Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, are cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs are generated using the LR recombination reaction between an attL-containing entry clone (see Example 11A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) is obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction is performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments result in a hairpin construct for each of the target genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example 11B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) is purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) is added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix is transformed into Top 10 chemically competent cells. Positive clones are selected by restriction digest analyses.
D. Laboratory Trials to Test dsRNA Targets, Using Artificial Diet for Activity Against Plutella xylostella Larvae
Diamond-back moths, Plutella xylostella, were maintained at Insect Investigations Ltd. (origin: Newcastle University, Newcastle-upon-Tyne, UK). The insects were reared on cabbage leaves. First instar, mixed sex larvae (approximately 1 day old) were selected for use in the trial. Insects were maintained in Eppendorf tubes (1.5 ml capacity). Commercially available Diamond-back moth diet (Bio-Serv, NJ, USA), prepared following the manafacturer's instructions, was placed in the lid of each tube (0.25 ml capacity, 8 mm diameter). While still liquid, the diet was smoother over to remove excess and produce an even surface.
Once the diet has set the test formulations are applied to the diet's surface, at the rate of 25 μl undiluted formulation (1 μg/μl dsRNA of targets) per replicate. The test formulations are allowed to dry and one first instar moth larva is placed in each tube. The larva is placed on the surface of the diet in the lid and the tube carefully closed. The tubes are stored upside down, on their lids such that each larva remains on the surface of the diet. Twice weekly the larvae are transferred to new Eppendorf tubes with fresh diet. The insects are provided with treated diet for the first two weeks of the trial and thereafter with untreated diet.
Assessments are made twice weekly for a total of 38 days at which point all larvae are dead. At each assessment the insects are assessed as live or dead and examined for abnormalities. Forty single larva replicates are performed for each of the treatments. During the trial the test conditions are 23 to 26° C. and 50 to 65% relative humidity, with a 16:8 hour light:dark photoperiod.

Example 12

Acheta domesticus (House Cricket)

A. Cloning Acheta domesticus Partial Sequences
High quality, intact RNA was isolated from all the different insect stages of Acheta domesticus (house cricket; source: Dr. Lara Senior, Insect Investigations Ltd., Capital Business Park, Wentloog, Cardiff, CF3 2PX, Wales, UK) using TRIzol Reagent (Cat. No. 15596-026/15596-018, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions. Genomic DNA present in the RNA preparation was removed by DNase treatment following the manafacturer's instructions (Cat. No. 1700, Promega). cDNA was generated using a commercially available kit (SuperScript™ III Reverse Transcriptase, Cat. No. 18080044, Invitrogen, Rockville, Md., USA) following the manufacturer's instructions.
To isolate cDNA sequences comprising a portion of the AD001, AD002, AD009, AD015 and AD016 genes, a series of PCR reactions with degenerate primers were performed using Amplitaq Gold (Cat. No. N8080240, Applied Biosystems) following the manafacturer's instructions.
The sequences of the degenerate primers used for amplification of each of the genes are given in Table 2-AD. These primers were used in respective PCR reactions with the following conditions: 10 minutes at 95° C., followed by 40 cycles of 30 seconds at 95° C., 1 minute at 50° C. and 1 minute and 30 seconds at 72° C., followed by 7 minutes at 72° C. The resulting PCR fragments were analyzed on agarose gel, purified (QIAquick Gel Extraction kit, Cat. No. 28706, Qiagen), cloned into the pCR8/GW/topo vector (Cat. No. K2500 20, Invitrogen) and sequenced. The sequences of the resulting PCR products are represented by the respective SEQ ID NOs as given in Table 2-AD and are referred to as the partial sequences. The corresponding partial amino acid sequence are represented by the respective SEQ ID NOs as given in Table 3-AD.
B. dsRNA Production of the Acheta domesticus Genes
dsRNA was synthesized in milligram amounts using the commercially available kit T7 Ribomax™ Express RNAi System (Cat. No. P1700, Promega). First two separate single 5′ T7 RNA polymerase promoter templates were generated in two separate PCR reactions, each reaction containing the target sequence in a different orientation relative to the T7 promoter.
For each of the target genes, the sense T7 template was generated using specific T7 forward and specific reverse primers. The sequences of the respective primers for amplifying the sense template for each of the target genes are given in Table 8-AD. The conditions in the PCR reactions were as follows: 1 minute at 95° C., followed by 20 cycles of 30 seconds at 95° C., 30 seconds at 60° C. (−0.5° C./cycle) and 1 minute at 72° C., followed by 15 cycles of 30 seconds at 95° C., 30 seconds at 50° C. and 1 minute at 72° C., followed by 10 minutes at 72° C. The anti-sense T7 template was generated using specific forward and specific T7 reverse primers in a PCR reaction with the same conditions as described above. The sequences of the respective primers for amplifying the anti-sense template for each of the target genes are given in Table 8-AD. The resulting PCR products were analyzed on agarose gel and purified by PCR purification kit (Qiaquick PCR Purification Kit, Cat. No. 28106, Qiagen) and NaClO₄precipitation. The generated T7 forward and reverse templates were mixed to be transcribed and the resulting RNA strands were annealed, DNase and RNase treated, and purified by sodium acetate, following the manufacturer's instructions. The sense strand of the resulting dsRNA for each of the target genes is given in Table 8-AD.
C. Recombination of the Acheta domesticus Genes into the Plant Vector pK7GWIWG2D(II)
Since the mechanism of RNA interference operates through dsRNA fragments, the target nucleotide sequences of the target genes, as selected above, are cloned in anti-sense and sense orientation, separated by the intron-CmR-intron, whereby CmR is the chloramphenicol resistance marker, to form a dsRNA hairpin construct. These hairpin constructs are generated using the LR recombination reaction between an attL-containing entry clone (see Example 12A) and an attR-containing destination vector (=pK7GWIWG2D(II)). The plant vector pK7GWIWG2D(II) is obtained from the VIB/Plant Systems Biology with a Material Transfer Agreement. LR recombination reaction is performed by using LR Clonase™ II enzyme mix (Cat. No. 11791-020, Invitrogen) following the manufacturer's instructions. These cloning experiments result in a hairpin construct for each of the target genes, having the promoter-sense-intron-CmR-intron-antisense orientation, and wherein the promoter is the plant operable 35S promoter. The binary vector pK7GWIWG2D(II) with the 35S promoter is suitable for transformation into A. tumefaciens.
Restriction enzyme digests were carried out on pCR8/GW/TOPO plasmids containing the different targets (see Example 12B). The band containing the gene of interest flanked by the attL sites using Qiaquick Gel Extraction Kit (Cat. No. 28706, Qiagen) is purified. An amount of 150 ng of purified fragment and 150 ng pK7GWIWG2D(II) is added together with the LR clonase II enzyme and incubated for at least 1 h at 25° C. After proteinase K solution treatment (10 min at 37° C.), the whole recombination mix is transformed into Top 10 chemically competent cells. Positive clones are selected by restriction digest analyses.
D. Laboratory Trials to Test dsRNA Targets, Using Artificial Diet for Activity Against Acheta domesticus Larvae
House crickets, Acheta domesticus, were maintained at Insect Investigations Ltd. (origin: Blades Biological Ltd., Kent, UK). The insects were reared on bran pellets and cabbage leaves. Mixed sex nymphs of equal size and no more than 5 days old were selected for use in the trial. Double-stranded RNA is mixed with a wheat-based pelleted rodent diet (rat and mouse standard diet, B & K Universal Ltd., Grimston, Aldbrough, Hull, UK). The diet, BK001P, contains the following ingredients in descending order by weight: wheat, soya, wheatfeed, barley, pellet binder, rodent 5 vit min, fat blend, dicalcium phosphate, mould carb. The pelleted rodent diet is finely ground and heat-treated in a microwave oven prior to mixing, in order to inactivate any enzyme components. All rodent diet is taken from the same batch in order to ensure consistency. The ground diet and dsRNA are mixed thoroughly and formed into small pellets of equal weight, which are allowed to dry overnight at room temperature.
Double-stranded RNA samples from targets and gfp control at concentrations 10 μg/μl were applied in the ratio 1 g ground diet plus 1 ml dsRNA solution, thereby resulting in an application rate of 10 mg dsRNA per g pellet. Pellets are replaced weekly. The insects are provided with treated pellets for the first three weeks of the trial. Thereafter untreated pellets are provided. Insects are maintained within lidded plastic containers (9 cm diameter, 4.5 cm deep), ten per container. Each arena contains one treated bait pellet and one water source (damp cotton wool ball), each placed in a separate small weigh boat. The water is replenished ad lib throughout the experiment.
Assessments are made at twice weekly intervals, with no more than four days between assessments, until all the control insects had either died or moulted to the adult stage (84 days). At each assessment the insects are assessed as live or dead, and examined for abnormalities. From day 46 onwards, once moulting to adult has commenced, all insects (live and dead) are assessed as nymph or adult. Surviving insects are weighed on day 55 of the trial. Four replicates are performed for each of the treatments. During the trial the test conditions are 25 to 33° C. and 20 to 25% relative humidity, with a 12:12 hour light:dark photoperiod.

TABLE 1A

C. elegans id	D. melanogaster id	description	devgen RNAi screen

B0250.1	CG1263	large ribosomal subunit L8 protein.	Acute lethal or lethal
B0336.10	CG3661	large ribosomal subunit L23 protein.	Acute lethal or lethal
B0336.2	CG8385	ADP-ribosylation factor	Acute lethal or lethal
B0464.1	CG3821	Putative aspartyl(D) tRNA synthetase.	Acute lethal or lethal
C01G8.5	CG10701	Ortholog of the ERM family of cytoskeletal linkers	Acute lethal or lethal
C01H6.5	CG33183	Nuclear hormone receptor that is required in all larval molts	Acute lethal or lethal
C02C6.1	CG18102	Member of the DYNamin related gene class	Acute lethal or lethal
C03D6.8	CG6764	Large ribosomal subunit L24 protein (Rlp24p)	Acute lethal or lethal
C04F12.4	CG6253	rpl-14 encodes a large ribosomal subunit L14 protein.	Acute lethal or lethal
C04H5.6	CG10689	Product with RNA helicase activity (EC:2.7.7.-) involved in nuclear	Embryonic lethal or sterile
		mRNA splicing, via spliceosome which is a component of the
		spliceosome complex
C13B9.3	CG14813	Delta subunit of the coatomer (COPI) complex	Acute lethal or lethal
C17H12.14	CG1088	Member of the Vacuolar H ATPase gene class	Acute lethal or lethal
C26E6.4	CG3180	DNA-directed RNA polymerase II	Acute lethal or lethal
F23F12.6	CG16916	Triple A ATPase subunit of the 26S proteasome's 19S regulatory particle	Acute lethal or lethal
		(RP) base subcomplex
F57B9.10	CG10149	Member of the proteasome Regulatory Particle, Non-ATPase-like gene	Acute lethal or lethal
		class
K11D9.2	CG3725	sarco-endoplasmic reticulum Ca[2+] ATPase homolog	Embryonic lethal or sterile
T20G5.1	CG9012	Clathrin heavy chain	Acute lethal or lethal
T20H4.3	CG5394	Predicted cytoplasmic prolyl-tRNA synthetase (ProRS)	Acute lethal or lethal
T21E12.4	CG7507	Cytoplasmic dynein heavy chain homolog	Acute lethal or lethal
C05C10.3	CG1140	Orthologue to the human gene 3-OXOACID COA TRANSFERASE	Acute lethal or lethal
C09D4.5	CG2746	Ribosomal protein L19, structural constituent of ribosome involved in	Acute lethal or lethal
		protein biosynthesis which is localised to the ribosome
C09E10.2	CG31140	Orthologue of diacylglyerol kinase involved in movement, egg laying, and	Acute lethal or lethal
		synaptic transmission, and is expressed in neurons.
C13B9.3	CG14813	Delta subunit of the coatomer (COPI)	Acute lethal or lethal
C14B9.7	CG12775	Large ribosomal subunit L21 protein (RPL-21) involved in protein	Acute lethal or lethal
		biosynthesis
C15H11.7	CG30382	Type	6 alpha subunit of the 26S proteasome's 20S protease core particle	Acute lethal or lethal
		(CP)
C17E4.9	CG9261	Protein involved with Na+/K+− exchanging ATPase complex	Embryonic lethal or sterile
C17H12.14	CG1088	V-ATPase E subunit	Acute lethal or lethal
C23G10.4	CG11888	Non-ATPase subunit of the 26S proteasome's 19S regulatory paritcle	Acute lethal or lethal
		base subcomplex (RPN-2)
C26D10.2	CG7269	Product with helicase activity involved in nuclear mRNA splicing, via	Acute lethal or lethal
		spliceosome which is localized to the nucleus
C26E6.4	CG3180	RNA polymerase II 140 kD subunit (RpII140), DNA-directed RNA	Acute lethal or lethal
		polymerase activity (EC:2.7.7.6) involved in transcription from Pol II
		promoter which is a component of the DNA-directed RNA polymerase II,
		core complex
C26F1.4	CG15697	Product with function in protein biosynthesis and ubiquitin in protein	Acute lethal or lethal
		degradation.
C30C11.1	CG12220	Unknown function	Acute lethal or lethal
C30C11.2	CG10484	Member of the proteasome Regulatory Particle, Non-ATPase-like gene	Acute lethal or lethal
		class
C36A4.2	CG13977	cytochrome P450	Acute lethal or lethal
C37C3.6	CG33103	Orthologous to thrombospondin, papilin and lacunin	Acute lethal or lethal
C37H5.8	CG8542	Member of the Heat Shock Protein gene class	Acute lethal or lethal
C39F7.4	CG3320	Rab-protein 1 involved in cell adhesion	Acute lethal or lethal
C41C4.8	CG2331	Transitional endoplasmic reticulum ATPase TER94, Golgi organization	Growth delay or arrested in
		and biogenesis	growth
C42D8.5	CG8827	ACE-like protein	Acute lethal or lethal
C47E12.5	CG1782	Ubiquitin-activating enzyme, function in an ATP-dependent reaction that	Acute lethal or lethal
		activates ubiquitin prior to its conjugation to proteins that will
		subsequently be degraded by the 26S proteasome.
C47E8.5	CG1242	Member of the abnormal DAuer Formation gene class	Acute lethal or lethal
C49H3.11	CG5920	Small ribosomal subunit S2 protein.	Acute lethal or lethal
C52E4.4	CG1341	Member of the proteasome Regulatory Particle, ATPase-like gene class	Acute lethal or lethal
C56C10.3	CG8055	Carrier protein with putatively involved in intracellular protein transport	Growth delay or arrested in
			growth
CD4.6	CG4904	Type	1 alpha subunit of the 26S proteasome's 20S protease core particle	Acute lethal or lethal
		(CP).
D1007.12	CG9282	Large ribosomal subunit L24 protein.	Acute lethal or lethal
D1054.2	CG5266	Member of the Proteasome Alpha Subunit gene class	Acute lethal or lethal
D1081.8	CG6905	MYB transforming protein	Acute lethal or lethal
F07D10.1	CG7726	Large ribosomal subunit L11 protein (RPL-11.2) involved in protein	Acute lethal or lethal
		biosynthesis.
F11C3.3	CG17927	Muscle myosin heavy chain (MHC B)	Acute lethal or lethal
F13B10.2	CG4863	Large ribosomal subunit L3 protein (rpl-3)	Acute lethal or lethal
F16A11.2	CG9987	Methanococcus hypothetical protein 0682 like	Acute lethal or lethal
F20B6.2	CG17369	V-ATPase B subunit	Growth delay or arrested in
			growth
F23F12.6	CG16916	Triple A ATPase subunit of the 26S proteasome's 19S regulatory particle	Acute lethal or lethal
		(RP) base subcomplex (RPT-3)
F25H5.4	CG2238	Translation elongation factor 2 (EF-2), a GTP-binding protein involved in	Growth delay or arrested in
		protein synthesis	growth
F26D10.3	CG4264	Member of the Heat Shock Protein gene class	Acute lethal or lethal
F28C6.7	CG6846	Large ribosomal subunit L26 protein (RPL-26) involved in protein	Embryonic lethal or sterile
		biosynthesis
F28D1.7	CG8415	Small ribosomal subunit S23 protein (RPS-23) involved in protein	Acute lethal or lethal
		biosynthesis
F29G9.5	CG5289	Member of the proteasome Regulatory Particle, ATPase-like gene class	Acute lethal or lethal
F32H2.5	CG3523	Mitochondrial protein	Acute lethal or lethal
F37C12.11	CG2986	Small ribosomal subunit S21 protein (RPS-21) involved in protein	Acute lethal or lethal
		biosynthesis
F37C12.4	CG7622	Large ribosomal subunit L36 protein (RPL-36) involved in protein	Acute lethal or lethal
		biosynthesis
F37C12.9	CG1527	Small ribosomal subunit S14 protein (RPS-14) involved in protein	Acute lethal or lethal
		biosynthesis
F38E11.5	CG6699	beta′ (beta-prime) subunit of the coatomer (COPI) complex	Acute lethal or lethal
F39B2.6	CG10305	Small ribosomal subunit S26 protein (RPS-26) involved in protein	Acute lethal or lethal
		biosynthesis
F39H11.5	CG12000	Member of the Proteasome Beta Subunit gene class	Acute lethal or lethal
F40F8.10	CG3395	Ribosomal protein S9 (RpS9), structural constituent of ribosome involved	Acute lethal or lethal
		in protein biosynthesis which is a component of the cytosolic small
		ribosomal subunit
F42C5.8	CG7808	Small ribosomal subunit S8 protein (RPS-8) involved in protein	Acute lethal or lethal
		biosynthesis
F49C12.8	CG5378	Member of the proteasome Regulatory Particle, Non-ATPase-like gene	Acute lethal or lethal
		class
F53A3.3	CG2033	Small ribosomal subunit S15a protein.	Acute lethal or lethal
F53G12.10	CG4897	large ribosomal subunit L7 protein (rpl-7)	Acute lethal or lethal
F54A3.3	CG8977	Unknown function	Acute lethal or lethal
F54E2.3	CG1915	Product with sallimus (sls), myosin-light-chain kinase activity
		(EC:2.7.1.117) involved in mitotic chromosome condensation which is
		localized to the nucleus
F54E7.2	CG11271	Small ribosomal subunit S12 protein (RPS-12) involved in protein	Acute lethal or lethal
		biosynthesis
F55A11.2	CG4214	Member of the SYNtaxin gene class	Acute lethal or lethal
F55A3.3	CG1828	transcritpion factor	Acute lethal or lethal
F55C10.1	CG11217	Ortholog of calcineurin B, the regulatory subunit of the protein	Acute lethal or lethal
		phosphatase 2B
F56F3.5	CG2168	rps-1 encodes a small ribosomal subunit S3A protein.	Acute lethal or lethal
F57B9.10	CG10149	Member of the proteasome Regulatory Particle, Non-ATPase-like gene	Acute lethal or lethal
		class
F58F12.1	CG2968	ATP synthase	Acute lethal or lethal
F59E10.3	CG3948	Zeta subunit of the coatomer (COPI) complex	Acute lethal or lethal
JC8.3	CG3195	Large ribosomal subunit L12 protein (rpl-12)	Acute lethal or lethal
K01G5.4	CG1404	Putative RAN small monomeric GTPase (cell adhesion)	Acute lethal or lethal
K04F10.4	CG18734	Subtilase	Acute lethal or lethal
K05C4.1	CG12323	Member of the Proteasome Beta Subunit gene class	Acute lethal or lethal
K07D4.3	CG18174	Putative proteasome regulatory particle, lid subcomplex, rpn11	Acute lethal or lethal
K11D9.2	CG3725	Sarco-endoplasmic reticulum Ca[2+] ATPase	Embryonic lethal or sterile;
			Acute lethal or lethal
M03F4.2	CG4027	An actin that is expressed in body wall and vulval muscles and the	Acute lethal or lethal
		spermatheca.
R06A4.9	CG1109	six WD40 repeats	Acute lethal or lethal
R10E11.1	CG15319	Putative transcriptional cofactor	Acute lethal or lethal
R12E2.3	CG3416	Protein with endopeptidase activity involved in proteolysis and	Acute lethal or lethal
		peptidolysis
F10C1.2	CG10119	Member of the Intermediate Filament, B gene class	Embryonic lethal or sterile
F35G12.8	CG11397	Homolog of the SMC4 subunit of mitotic condensin	Embryonic lethal or sterile
F53G12.1	CG5771	GTPase homologue	Embryonic lethal or sterile
F54E7.3	CG5055	PDZ domain-containing protein	Embryonic lethal or sterile
H28O16.1	CG3612	ATP synthase	Growth delay or arrested in
			growth
K12C11.2	CG4494	Member of the SUMO (ubiquitin-related) homolog gene class	Embryonic lethal or sterile
R12E2.3	CG3416	Member of the proteasome Regulatory Particle, Non-ATPase-like gene	Acute lethal or lethal
		class
R13A5.8	CG6141	Ribosomal protein L9, structural constituent of ribosome involved in	Acute lethal or lethal
		protein biosynthesis which is localised to the ribosome
T01C3.6	CG4046	rps-16 encodes a small ribosomal subunit S16 protein.	Acute lethal or lethal
T01H3.1	CG7007	proteolipid protein PPA1 like protein	Acute lethal or lethal
T05C12.7	CG5374	Cytosolic chaperonin	Acute lethal or lethal
T05H4.6	CG5605	eukaryotic peptide chain release factor subunit 1	Acute lethal or lethal
T10H9.4	CG17248	N-synaptobrevin; v-SNARE, vesicle-mediated transport, synaptic vesicle
T14F9.1	CG17332	ATPase subunit	Growth delay or arrested in
			growth
T20G5.1	CG9012	Clathrin heavy chain	Acute lethal or lethal
T21B10.7	CG7033	t-complex protein 1	Embryonic lethal or sterile
W09B12.1	CG17907	Acetylcholineesterase
T27F2.1	CG8264	Member of the mammalian SKIP (Ski interacting protein) homolog gene	Acute lethal or lethal
		class
ZC434.5	CG5394	predicted mitochondrial glutamyl-tRNA synthetase (GluRS)	Acute lethal or lethal
B0511.6	CG6375	helicase	Embryonic lethal or sterile
DY3.2	CG10119	Nuclear lamin; LMN-1 protein	Growth delay or arrested in
			growth
R13G10.1	CG11397	homolog of the SMC4 subunit of mitotic condensin	Wild Type
T26E3.7	CG3612	Predicted mitochondrial protein.	Growth delay or arrested in
			growth
Y113G7A.3	CG1250	GTPase activator, ER to Golgi prot transport, component of the Golgi	Acute lethal or lethal
		stack
Y43B11AR.4	CG11276	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved	Acute lethal or lethal
		in protein biosynthesis which is a component of the cytosolic small
		ribosomal subunit
Y46G5A.4	CG5931	Y46G5A.4 gene	Acute lethal or lethal
Y71F9AL.17	CG7961	Alpha subunit of the coatomer (COPI) complex	Acute lethal or lethal
Y76B12C.7	CG10110	Gene cleavage and polyadenylation specificity factor	Embryonic lethal or sterile
Y37D8A.10	CG1751	Unknown function	Embryonic lethal or sterile
CG7765	C06G3.2	Member of the Kinesin-Like Protein gene class
CG10922	C44E4.4	RNA-binding protein	Embryonic lethal or sterile
CG4145	F01G12.5	alpha-2 type IV collagen	Embryonic lethal or sterile
CG13391	F28H1.3	apredicted cytoplasmic alanyl-tRNA synthetase (AlaRS)	Growth delay or arrested in
			growth
CG7765	R05D3.7	Member of the UNCoordinated gene class	Embryonic lethal or sterile
CG7398	R06A4.4	Member of the IMportin Beta family gene class	Embryonic lethal or sterile
CG7436	T17E9.2	Unknown function	Embryonic lethal or sterile
CG2666	T25G3.2	putative chitin synthase	Embryonic lethal or sterile
CG17603	W04A8.7	TATA-binding protein associated factor TAF1L (TAFII250)	Embryonic lethal or sterile

TABLE 1-LD

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

LD001	CG11276	1	2	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
LD002	CG8055	3	4	Carrier protein with putatively involved in intracellular protein transport
LD003	CG3395	5	6	Ribosomal protein S9 (RpS9), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
LD006	CG3180	7	8	RNA polymerase II 140 kD subunit (RpII140), DNA-directed RNA polymerase activity (EC:2.7.7.6)
				involved in transcription from Pol II promoter which is a component of the DNA-directed RNA
				polymerase II, core complex
LD007	CG7269	9	10	Helicase at 25E (Hel25E), also known in FlyBase as Dbp25F, Hel, I(2)25Eb and I(2)k11511, pre-
				mRNA splicing factor activity involved in nuclear mRNA splicing, via spliceosome which is localized
				to the nucleus
LD010	CG1250	11	12	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
LD011	CG1404	13	14	Tutative RAN small monomeric GTPase (cell adhesion)
LD014	CG1088	15	16	V-ATPase E subunit
LD015	CG2331	17	18	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis
LD016	CG17369	19	20	V-ATPase B subunit
LD018	CG1915	21	22	Sallimus (sls), myosin-light-chain kinase activity (EC:2.7.1.117) involved in mitotic chromosome
				condensation which is localized to the nucleus
LD027	CG6699	23	24	Beta-coatamer protein, subunit of a multimeric complex that forms a membrane vesicle coat

TABLE 1-PC

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

PC001	CG11276	247	248	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
PC003	CG3395	249	250	Ribosomal protein S9 (RpS9), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
PC005	CG2746	251	252	Ribosomal protein L19, structural constituent of ribosome involved in protein biosynthesis which is
				localised to the ribosome
PC010	CG1250	253	254	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
PC014	CG1088	255	256	V-ATPase E subunit
PC016	CG17369	257	258	V-ATPase B subunit
PC027	CG6699	259	260	Beta-coatamer protein, subunit of a multimeric complex that forms a membrane vesicle coat

TABLE 1-EV

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

EV005	CG2746	513	514	Ribosomal protein L19, structural constituent of ribosome involved in protein biosynthesis which is
				localised to the ribosome
EV009	CG9261	515	516	Protein involved with Na+/K+− exchanging ATPase complex
EV010	CG1250	517	518	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
EV015	CG2331	519	520	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis
EV016	CG17369	521	522	V-ATPase B subunit

TABLE 1-AG

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

AG001	CG11276	601	602	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
AG005	CG2746	603	604	Ribosomal protein L19, structural constituent of ribosome involved in protein biosynthesis which is
				localised to the ribosome
AG010	CG1250	605	606	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
AG014	CG1088	607	608	V-ATPase E subunit
AG016	CG17369	609	610	V-ATPase B subunit

TABLE 1-TC

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

TC001	CG11276	793	794	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
TC002	CG8055	795	796	Protein with putatively involved in intracellular protein transport
TC010	CG1250	797	798	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
TC014	CG1088	799	800	V-ATPase E subunit
TC015	CG2331	801	802	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis

TABLE 1-MP

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

MP001	CG11276	888	889	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
MP002	CG8055	890	891	Carrier protein with putatively involved in intracellular protein transport
MP010	CG1250	892	893	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
MP016	CG17369	894	895	V-ATPase B subunit
MP027	CG6699	896	897	Beta-coatamer protein, subunit of a multimeric complex that forms a membrane vesicle coat

TABLE 1-NL

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

NL001	CG11276	1071	1072	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis which
				is a component of the cytosolic small ribosomal subunit
NL002	CG8055	1073	1074	Protein with putatively involved in intracellular protein transport
NL003	CG3395	1075	1076	Ribosomal protein S9 (RpS9), structural constituent of ribosome involved in protein biosynthesis which
				is a component of the cytosolic small ribosomal subunit
NL004	CG6141	1077	1078	Ribosomal protein L9, structural constituent of ribosome involved in protein biosynthesis which is
				localised to the ribosome
NL005	CG2746	1079	1080	Ribosomal protein L19, structural constituent of ribosome involved in protein biosynthesis which is
				localised to the ribosome
NL006	CG3180	1081	1082	RNA polymerase II 140 kD subunit (RpII140), DNA-directed RNA polymerase activity (EC:2.7.7.6)
				involved in transcription from Pol II promoter which is a component of the DNA-directed RNA
				polymerase II, core complex
NL007	CG7269	1083	1084	Helicase at 25E (Hel25E), also known in FlyBase as Dbp25F, Hel, I(2)25Eb and I(2)k11511, pre-
				mRNA splicing factor activity involved in nuclear mRNA splicing, via spliceosome which is localized to
				the nucleus
NL008	CG3416	1085	1086	Protein with endopeptidase activity involved in proteolysis and peptidolysis which is a component of
				the proteasome regulatory particle, lid subcomplex (sensu Eukarya)
NL009	CG9261	1087	1088	Protein involved with Na+/K+− exchanging ATPase complex
NL010	CG1250	1089	1090	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
NL011	CG1404	1091	1092	Putative RAN small monomeric GTPase (cell adhesion)
NL012	CG17248	1093	1094	N-synaptobrevin; v-SNARE, vesicle-mediated transport, synaptic vesicle
NL013	CG18174	1095	1096	Putative proteasome regulatory particle, lid subcomplex, rpn11
NL014	CG1088	1097	1098	V-ATPase E subunit
NL015	CG2331	1099	1100	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis
NL016	CG17369	1101	1102	V-ATPase B subunit
NL018	CG1915	1103	1104	Sallimus (sls), myosin-light-chain kinase activity (EC:2.7.1.117) involved in mitotic chromosome
				condensation which is localized to the nucleus
NL019	CG3320	1105	1106	Rab-protein 1 involved in cell adhesion
NL021	CG10110	1107	1108	Gene cleavage and polyadenylation specificity factor
NL022	CG10689	1109	1110	Product with RNA helicase activity (EC:2.7.7.-) involved in nuclear mRNA splicing, via spliceosome
				which is a component of the spliceosome complex
NL023	CG17907	1111	1112	Acetylcholineesterase
NL027	CG6699	1113	1114	Beta-coatomer protein

TABLE 1-CS

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

CS001	CG11276	1682	1683	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
CS002	CG8055	1684	1685	Carrier protein with putatively involved in intracellular protein transport
CS003	CG3395	1686	1687	Ribosomal protein S9 (RpS9), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
CS006	CG3180	1688	1689	RNA polymerase II 140 kD subunit (RpII140), DNA-directed RNA polymerase activity (EC:2.7.7.6)
				involved in transcription from Pol II promoter which is a component of the DNA-directed RNA
				polymerase II, core complex
CS007	CG7269	1690	1691	Helicase at 25E (Hel25E), also known in FlyBase as Dbp25F, Hel, I(2)25Eb and I(2)k11511, pre-
				mRNA splicing factor activity involved in nuclear mRNA splicing, via spliceosome which is localized
				to the nucleus
CS009	CG9261	1692	1693	Protein involved with Na+/K+− exchanging ATPase complex
CS011	CG1404	1694	1695	Tutative RAN small monomeric GTPase (cell adhesion)
CS013	CG18174	1696	1697	Putative proteasome regulatory particle, lid subcomplex, rpn11
CS014	CG1088	1698	1699	V-ATPase E subunit
CS015	CG2331	1700	1701	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis
CS016	CG17369	1702	1703	V-ATPase B subunit
CS018	CG1915	1704	1705	Sallimus (sls), myosin-light-chain kinase activity (EC:2.7.1.117) involved in mitotic chromosome
				condensation which is localized to the nucleus

TABLE 1-PX

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

PX001	CG11276	2100	2101	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
PX009	CG9261	2102	2103	Protein involved with Na+/K+− exchanging ATPase complex
PX010	CG1250	2104	2105	GTPase activator, ER to Golgi prot transport, component of the Golgi stack
PX015	CG2331	2106	2107	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis
PX016	CG17369	2108	2109	V-ATPase B subunit

TABLE 1-AD

	Dm	SEQ ID	SEQ ID
Target ID	identifier	NO NA	NO AA	Function (based on Flybase)

AD001	CG11276	2364	2365	Ribosomal protein S4 (RpS4), structural constituent of ribosome involved in protein biosynthesis
				which is a component of the cytosolic small ribosomal subunit
AD002	CG8055	2366	2367	Carrier protein with putatively involved in intracellular protein transport
AD009	CG9261	2368	2369	Protein involved with Na+/K+− exchanging ATPase complex
AD015	CG2331	2370	2371	Transitional endoplasmic reticulum ATPase TER94, Golgi organization and biogenesis
AD016	CG17369	2372	2373	V-ATPase B subunit

TABLE 2-LD

	Primer
	Forward	Primer Reverse	cDNA Sequence (sense strand)
Target ID	5′ → 3′	5′ → 3′	5′ → 3′

LD001	SEQ ID NO: 25	SEQ ID NO: 26	SEQ ID NO: 1
	GGCCCCAAGAA	TAGCGGATGGT	GGCCCCAAGAAGCATTTGAAGCGTTTGAATGCCCCAAAAGCATGGATGTTGGATAAATTGG
	GCATTTGAAGCG	GCGDCCRTCRTG	GAGGTGTTTTCGCACCTCGCCCATCTACAGGACCTCACAAATTGCGAGAGTCTTTGCCCTT
			GGTGATCTTCCTACGTAACCGATTGAAGTATGCTTTGACTAACAGCGAAGTTACTAAGATTG
			TTATGCAAAGGTTAATCAAAGTAGATGGAAAAGTGAGGACCGACTCCAATTACCCTGCTGG
			GTTTATGGATGTTATTACCATTGAAAAAACTGGTGAATTTTTCCGACTCATCTATGATGTTAA
			AGGACGATTTGCAGTGCATCGTATTACTGCTGAGGAAGCAAAGTACAAACTATGCAAAGTC
			AGGAGGATGCAAACTGGCCCCAAAGGAATTCCCTTCATAGTGACACACGACGGCCGCACC
			ATCCGCTA

LD002	SEQ ID NO: 27	SEQ ID NO: 28	SEQ ID NO: 3
	GAGCGGCCAT	GCAATGTCATC	GCAATGTCATCCATCATGTCGTGTACATTGTCCACGTCCAAGTTTTTATGGGCTTTCTTAAG
	GCAAGCVCTBA	CATCAKRTCRT	AGCTTCAGCTGCATTTTTCATAGATTCCAATACTGTGGTGTTCGTACTAGCTCCCTCCAGAG
	ARMRRAAG	GCAC	CTTCTCGTTGAAGTTCAATAGTAGTTAAAGTGCCATCTATTTGCAACTGATTTTTTTCTAATC
			GCTTCTTCCGCTTCAGCGCTTGCATGGCCGCTC

LD003	SEQ ID NO: 29	SEQ ID NO: 30	SEQ ID NO: 5
	TCGGTCTTCTC	CAGGTTCTTCC	CAGGTTCTTCCTCTTGACGCGTCCAGGGCGACCACCACCGAATGGAGATTTGAGCGAGAA
	GAAGACNTAYG	TCTTKACRCGD	GTCAATATGCTTCTGGGAATCAAGTCTCACAATGAAGCTTGGAATATTCACGACCTGCTTAC
	TKAC	CC	GAACCCTGATATGTCTTTGACGGACCAGCACACGAGCATGATGGATTGATTTTGCAAGCCC
			CAACTTGAAAACTTGTGTTTGGAGACGTCGTTCCAAGAAATCTTCAATCTTCAAACCCAAGA
			CGTAATCAAGCTTCATACGGGTTTCATCCAACACTCCAATACGCACCAACCGACGAAGAAG
			AGCATTGCCTTCAAACAACCTGCGCTGATCTTTCTCTTCCAAAGTCAGAAGTTCTCTGGCAG
			CTTTACGGATTTTTGCCAAGGTATACTTGACTCGCCACACTTCACGTTTGTTCCTAAGACCA
			TATTCTCCTATGATTTTCAACTCCTGATCAAGACGTGCCTTTTCATAAGGTCGCCTGGGA

LD006	SEQ ID NO: 31	SEQ ID NO: 32	SEQ ID NO: 7
	GGAGCGAGAC	CTCGAACTGCT	GGAGCGAGACTACACAACTATGGCTGGCAGGTGTTGGTTGCTTCTGGTGTGGTGGAATAC
	TACAACAAYKA	CYTCYTGATCR	ATCGACACTCTTGAAGAAGAAACTGTCATGATTGCGATGAATCCTGAGGATCTTCGGCAGG
	YRGYTGGC	CC	ACAAAGAATATGCTTATTGTACGACCTACACCCACTGCGAAATCCACCCGGCCATGATCTT
			GGGCGTTTGCGCGTCTATTATACCTTTCCCCGATCATAACCAGAGCCCAAGGAACACCTAC
			CAGAGCGCTATGGGTAAGCAAGCTATGGGGGTCTACATTACGAATTTCCACGTGCGGATG
			GACACCCTGGCCCACGTGCTATACTACCCGCACAAACCTCTGGTCACTACCAGGTCTATG
			GAGTATCTGCGGTTCAGAGAATTACCAGCCGGGATCAACAGTATAGTTGCTATTGCTTGTT
			ATACTGGTTATAATCAAGAAGATTCTGTTATTCTGAACGCGTCTGCTGTGGAAAGAGGATTT
			TTCCGATCCGTGTTTTATCGTTCCTATAAAGATGCCGAATCGAAGCGAATTGGCGATCAAG
			AAGAGCAGTTCGAG

LD007	SEQ ID NO: 33	SEQ ID NO: 34	SEQ ID NO: 9
	CCGAAGAAGGA	CGATGCAAGTA	CCGAAGAAGGATGTGAAGGGTACTTACGTATCCATACACAGTTCAGGCTTCAGAGATTTTT
	YGTSAAGGGYAC	GGTGTCKGART	TATTGAAACCAGAAATTCTAAGAGCTATAGTTGACTGCGGTTTTGAACACCCTTCAGAAGTT
		CYTC	CAGCACGAATGTATTCCTCAAGCTGTCATTGGCATGGACATTTTATGTCAAGCCAAATCTGG
			TATGGGCAAAACGGCAGTGTTTGTTCTGGCGACACTGCAACAATTGGAACCAGCGGACAAT
			GTTGTTTACGTTTTGGTGATGTGTCACACTCGTGAACTGGCTTTCCAAATCAGCAAAGAGTA
			CGAGAGGTTCAGTAAATATATGCCCAGTGTCAAGGTGGGCGTCTTTTTCGGAGGAATGCCT
			ATTGCTAACGATGAAGAAGTATTGAAAAACAAATGTCCACACATTGTTGTGGGGACGCCTG
			GGCGTATTTTGGCGCTTGTCAAGTCTAGGAAGCTAGTCCTCAAGAACCTGAAACACTTCAT
			TCTTGATGAGTGCGATAAAATGTTAGAACTGTTGGATATGAGGAGAGACGTCCAGGAAATC
			TACAGAAACACCCCTCACACCAAGCAAGTGATGATGTTCAGTGCCACACTCAGCAAAGAAA
			TCAGGCCGGTGTGCAAGAAATTCATGCAAGATCCAATGGAGGTGTATGTAGACGATGAAG
			CCAAATTGACGTTGCACGGATTACAACAGCATTACGTTAAACTCAAAGAAAATGAAAAGAAT
			AAAAAATTATTTGAGTTGCTCGATGTTCTCGAATTTAATCAGGTGGTCATTTTTGTGAAGTCC
			GTTCAAAGGTGTGTGGCTTTGGCACAGTTGCTGACTGAACAGAATTTCCCAGCCATAGGAA
			TTCACAGAGGAATGGACCAGAAAGAGAGGTTGTCTCGGTATGAGCAGTTCAAAGATTTCCA
			GAAGAGAATATTGGTAGCTACGAATCTCTTTGGGCGTGGCATGGACATTGAAAGGGTCAAC
			ATTGTCTTCAACTATGATATGCCAGAGGACTCCGACACCTACTTGCATCG

LD010	SEQ ID NO: 35	SEQ ID NO: 36	SEQ ID NO: 11
	CTCTCAAGGAT	CGCCATTGGGC	CTCTCAAGGATTCGTTGCAGATGTCTTTGAGCTTGTTGCCCCCGAATGCCTTGATAGGGTT
	TCKYTRCARAT	RATGGTYTCKCC	GATTACCTTTGGGAAGATGGTCCAAGTGCACGAACTAGGTACCGAGGGCTGCAGCAAATC
	GTC		TTACGTTTTCCGAGGGACGAAAGACCTCACAGCTAAGCAAGTTCAAGAGATGTTGGAAGTG
			GGCAGAGCCGCAGTAAGTGCTCAACCTGCTCCTCAACAACCAGGACAACCCATGAGGCCT
			GGAGCACTCCAGCAAGCTCCTACGCCACCAGGAAGCAGGTTCCTTCAACCCATCTCGAAA
			TGCGACATGAACCTCACTGATCTTATTGGAGAGTTGCAAAGAGACCCATGGCCTGTCCACC
			AAGGCAAATGCGCCCTTAGATCGACCGGGACAGCTTTATCGATAGCCATTGGGTTGTTGGA
			GTGCACATACGCCAATACTGGTGCCAGGGTCATGCTATTCGTTGGAGGACCTTGCTCTCAA
			GGCCCTGGTCAAGTCTTGAATGATGATCTGAAGCAACCTATCAGATCTCACCACGACATCC
			AAAAAGACAATGCCAAATACATGAAGAAAGCAATCAAGCACTATGATAATTTAGCGATGAGA
			GCAGCAACGAATGGCCACTGCGTTGACATATATTCATGCGCTTTGGATCAGACAGGATTGA
			TGGAGATGAAACAGTGTTGTAATTCAACAGGGGGACATATGGTCATGGGCGACTCGTTCAA
			TTCTTCCCTGTTCAAGCAAACGTTCCAGCGCATATTTTCGAAAGATCAGAAAAACGAGCTGA
			AGATGGCATTTAATGGTACTCTGGAGGGTCAAGTGTTCCAGGGAGTTGAAAATTCAAGGCG
			GTATTGGATCTTGTGTTTCGTTGAATGTGAAGAATCCTTTGGTTTCCGACACCGAAATAGGA
			ATGGGTAACACGGTCCAGTGGAAAATGTGTACGGTAACTCCAAGTACTACCATGGCCTTGT
			TCTTCGAGGTCGTCAACCAACATTCCGCTCCCATACCTCAAGGGGGAAGGGGCTGCATAC
			AGTTCATCACGCAATATCAGCATGCTAGTGGCCAGAAGAGGATCCGAGTAACGACAGTTGC
			TAGAAACTGGGCCGATGCTTCCGCTAATATACATCATGTCAGTGCTGGATTCGATCAGGAG
			GCAGCCGCAGTGATAATGGCGAGGATGGCAGTTTACAGAGCGGAATCAGACGATAGCCCT
			GATGTTTTGAGATGGGTCGATAGGATGTTGATACGTCTGTGCCAGAAATTCGGCGAATATA
			ACAAGGACGACCCGAATTCGTTCCGCTTGGGCGAAAACTTCAGCCTCTACCCGCAGTTCAT
			GTACCATTTGAGAAGGTCACAGTTCCTGCAGGTGTTTAACAATTCTCCCGACGAAACGTCC
			TTCTACAGGCACATGCTTATGCGCGAAGACCTCACGCAGTCGCTGATCATGATCCAGCCGA
			TACTCTACAGCTACAGTTTCAATGGACCACCAGAACCTGTGCTTTTGGATACGAGTTCCATC
			CAACCCGATAGAATTCTGCTCATGGACACGTTCTTCCAGATTCTGATATTCCATGGCGAAAC
			CATCGCCCAATGGCG

LD011	SEQ ID NO: 37	SEQ ID NO: 38	SEQ ID NO: 13
	CCCACTTTCAA	GTGGAAGCAG	GTGGAAGCAGGGCTGGCATGGCGACAAATTCTAGATTGGGATCACCAATAAGCTTCCTAG
	GTGYGTRYTRG	GGCWGGCATK	CTAGCCATAGGAAAGGCTTCTCAAAGTTGTAGTTAGATTTGGCAGAGATATCATAGTACTGC
	TCGG	GCRAC	AAATTCTTCTTCCTATGAAAGACAATACTTTTCGCTTTTACTTTTCTGTCTTTGATGTCAACCT
			TGTTCCCGCAAAGTACTATCGGGATATTTTCACAGACTCTGACAAGATCTCTGTGCCAATTT
			GGTACATTCTTGTATGTAACTCTGGAAGTTACATCAAACATGATAATAGCACACTGTCCCTG
			AATGTAATATCCATCACGGAGACCACCAAACTTCTCCTGACCGGCAGTGTCCCATACATTG
			AACCGAATAGGGCCCCTGTTTGTATGGAAGACCAGAGGATGGACTTCAACTCCCAAAGTAG
			CTACATATCTTTTTTCAAATTCACCAGTCATATGACGTTTCACAAATGTCGTTTTTCCAGTAC
			CTCCATCTCCGACCAACACACACTTGAAAGTGGG

LD014	SEQ ID NO: 39	SEQ ID NO: 40	SEQ ID NO: 15
	CGCAGATCAAR	CGGATCTCGG	CGCAGATCAAGCATATGATGGCTTTCATTGAACAAGAGGCAAACGAAAAGGCAGAAGAAAT
	CAYATGATGGC	GCASMARYTGC	CGATGCCAAGGCCGAGGAAGAATTTAATATTGAAAAGGGGCGCCTTGTTCAGCAACAACGT
			CTCAAGATTATGGAATATTATGAGAAGAAAGAGAAACAGGTCGAACTCCAGAAAAAAATCCA
			ATCGTCTAACATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTT
			CGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGACCAGGGAAAA
			TATTCCCAAATCCTGGAAAGCCTCATTTTGCAGGGATTATATCAGCTTTTTGAGAAAGATGT
			TACCATTCGAGTTCGGCCCCAGGACCGAGAACTGGTCAAATCCATCATTCCCACCGTCACG
			AACAAGTATAAAGATGCCACCGGTAAGGACATCCATCTGAAAATTGATGACGAAATCCATCT
			GTCCCAAGAAACCACCGGGGGAATCGACCTGCTGGCGCAGAAAAACAAAATCAAGATCAG
			CAATACTATGGAGGCTCGTCTGGAGCTGATTTCGCAGCAACTTCTGCCCGAGATCCG

LD014_F1			SEQ ID NO: 159
			TCTAGAATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTA
			CCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGCCCGGG

LD014_F2			SEQ ID NO: 160
			TCTAGAAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCA
			CAAACGCCCGGG

LD014_C1			SEQ ID NO: 161
			TCTAGAATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTA
			CCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGATGTTGAATCAGGCT
			CGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGT
			AAACGACTTGGTCAGGTCACAAACGATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTT
			AGGGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACA
			AACGCCCGGG

LD014_C2			SEQ ID NO: 162
			TCTAGAAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCA
			CAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCA
			CAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCA
			CAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCA
			CAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCA
			CAAACGCCCGGG

LD015	SEQ ID NO: 41	SEQ ID NO: 42	SEQ ID NO: 17
	CGCCATCCRTC	GCAATGGCATC	GCAATGGCATCAAGTTCATCGATGAAGATGATCGCCGGAGAGTTTTTGTCAGCTTCTTCAA
	GCTSTTCAAGGC	AAKYTCRTCRA	AAGCTTTGCGCAAGTTACTCTCAGACTCGCCAGCGAGTTTGCTCATGATCTCCGGCCCGTT
		TG	TATCAAGAAGAAGAACGCCCCAGTCTCATTAGCCACGGCGCGAGCAATCAGGGTCTTACC
			CGTACCAGGGGGACCATACAGCAGTATACCCCTAGGGGGCTTCACGCCGATAGCCTTGAA
			GAGCGATGGATGGCG

LD016	SEQ ID NO: 43	SEQ ID NO: 44	SEQ ID NO: 19
	GACTGTGTCTG	GGAATAGGATG	GGAATAGGATGGGTAATGTCGTCGTTGGGCATAGTCAATATAGGAATCTGGGTGATGGATC
	GTGTRAACGG	GGTRATRTCGT	CGTTACGTCCTTCAACACGGCCGGCACGTTCATAGATGGTAGCTAAATCGGTGTACATGTA
	WCC	CG	ACCTGGGAAACCACGACGACCAGGCACCTCTTCTCTGGCAGCAGATACCTCACGCAAAGC
			TTCTGCATACGAAGACATATCTGTCAAGATGACCAAGACGTGCTTCTCACATTGGTAAGCC
			AAGAATTCGGCAGCTGTCAAAGCCAGACGAGGTGTAATAATTCTTTCAATGGTAGGATCGT
			TGGCCAAATTCAAGAACAGGCAGACATTCTCCATAGAACCGTTCTCTTCGAAATCCTGTTTG
			AAGAACCTAGCTGTTTCCATGTTAACACCCATAGCAGCGAAAACAATAGCAAAGTTATCTTC
			ATGATCATCAAGTACAGATTTACCAGGAATCTTGACTAAACCAGCCTGTCTACAGATCTGGG
			CAGCAATTTCATTGTGAGGCAGACCAGCTGCAGAGAAAATGGGGATCTTCTGACCACGAG
			CAATGGAGTTCATCACGTCAATAGCTGTAATACCCGTCTGGATCATTTCCTCAGGATAGATA
			CGGGACCACGGATTGATTGGTTGACCCTGGATGTCCAAGAAGTCTTCAGCCAAAATTGGG
			GGACCTTTGTCGATGGGTTTTCCTGATCCATTGAAAACACGTCCCAACATATCTTCAGAAAC
			AGGAGTCCTCAAAATATCTCCTGTGAATTCACAAGCGGTGTTTTTGGCGTCGATTCCTGAT
			GTGCCCTCGAACACTTGAACCACAGCTTTTGACCCACTGACTTCCAGAACTTGTCCCGAAC
			GTATAGTGCCATCAGCCAGTTTGAGTTGTACGATTTCATTGTACTTGGGGAACTTAACATCT
			TCGAGGATTACCAGAGGACCGTTCACACCAGACACAGTC

LD018	SEQ ID NO: 45	SEQ ID NO: 46	SEQ ID NO: 21
	CACCTGGTTCA	GTGCATCGGTA	CACCTGGTTCAAGGATGGGCAGCGGATAACGGAGTCGCAGAAATACGAGAGCACCTTCTC
	AGRATGGVCAR	CCAHSCHGCRTC	GAACAACCAAGCCTCCTTGAGGGTAAAACAAGCCCAGTCTGAGGACTCGGGACACTACAC
	MG		TTTGTTGGCGGAGAACCCTCAAGGCTGCATAGTGTCATCTGCTTACTTAGCCATAGAACCG
			GTAACCACCCAGGAAGGGTTGATCCACGAGTCCACCTTCAAGCAGCAACAGACCGAAATG
			GAGCAAATCGACACCAGCAAGACCTTGGCGCCTAACTTCGTCAGGGTTTGCGGGGATAGA
			GACGTGACCGAGGGCAAGATGACCCGCTTCGACTGTCGCGTCACTGGTCGTCCTTATCCA
			GACGTGACATGGTACATAAACGGTCGACAAGTCACCGACGACCACAACCACAAGATTTTGG
			TTAACGAATCCGGAAACCATGCCCTGATGATCACCACCGTGAGCAGGAACGACTCAGGAG
			TAGTGACCTGCGTCGCCAGGAACAAGACGGGAGAAACCTCCTTCCAGTGCAACCTTAACG
			TCATCGAAAAGGAACAGGTAGTCGCGCCCAAGTTCGTGGAGAGATTTACCACAGTCAACGT
			GGCAGAAGGAGAACCAGTGTCTCTGCGCGCTAGAGCTGTTGGCACGCCGGTGCCGCGAA
			TCACTTGGCAGAGGGACGGGGCGCCCCTAGCCAGCGGGCCCGACGTTCGCATCGCGATT
			GACGGTGGAGCCTCTACTTTGAATATCTCGAGGGCCAAGGCCTCGGATGCTGCATGGTAC
			CGATGCAC

LD027	SEQ ID NO: 47	SEQ ID NO: 48	SEQ ID NO: 23
	CCATGGTGGC	GGTATAGATGA	CCATGGTGGCGATAAACCATACTTGATATCGGGAGCAGACGATCGGTTGGTTAAAATCTGG
	GAYAARCCVTAC	ARCARTCDCCV	GACTATCAAAACAAAACGTGTGTCCAAACCTTGGAAGGACACGCCCAAAACGTAACCGCG
		ACCCA	GTTTGTTTCCACCCTGAACTACCTGTGGCTCTCACAGGCAGCGAAGATGGTACCGTTAGAG
			TTTGGCATACGAATACACACAGATTAGAGAATTGTTTGAATTATGGGTTCGAGAGAGTGTG
			GACCATTTGTTGCTTGAAGGGTTCGAATAATGTTTCTCTGGGGTATGACGAGGGCAGTATA
			TTAGTGAAAGTTGGAAGAGAAGAACCGGCAGTTAGTATGGATGCCAGTGGCGGTAAAATAA
			TTTGGGCAAGGCACTCGGAATTACAACAAGCTAATTTGAAGGCGCTGCCAGAAGGTGGAG
			AAATAAGAGATGGGGAGCGTTTACCTGTCTCTGTAAAAGATATGGGAGCATGTGAAATATA
			CCCTCAAACAATCCAACATAATCCGAATGGAAGATTCGTTGTAGTATGCGGAGACGGCGAA
			TATATCATTTACACAGCGATGGCTCTACGGAACAAGGCTTTTGGAAGCGCTCAAGAGTTTG
			TCTGGGCTCAGGACTCCAGCGAGTATGCCATTCGCGAGTCTGGTTCCACAATTCGGATATT
			CAAAAACTTCAAAGAAAGGAAGAACTTCAAGTCGGATTTCAGCGCGGAAGGAATCTACGGG
			GGTTTTCTCTTGGGGATTAAATCGGTGTCCGGTTTAACGTTTTACGATTGGGAAACTTTGGA
			CTTGGTGAGACGGATTGAAATACAACCGAGGGCGGTTTATTGGTCTGACAGTGGAAAATTA
			GTCTGTCTCGCAACGGAGGACAGCTACTTCATCCTTTCTTATGATTCGGAGCAAGTTCAGA
			AGGCCAGGGAGAACAATCAAGTCGCAGAGGATGGCGTAGAGGCCGCTTTCGATGTGTTGG
			GGGAAATGAACGAGTCTGTCCGAACAGGTCTTTGGGTCGGAGACTGTTTCATCTATACC

TABLE 2-PC

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

PC001	SEQ ID NO: 261	SEQ ID NO: 262	SEQ ID NO: 247
	CATTTGAAGCG	CTTCGTGCCCT	CATTTGAAGCGTTTAGCTGCTCCCAAAGCATGGATGTTGGACAAATTGGGGGGTGTCTTCGCCC
	TTTWRMYGCY	TGCCRATKATR	CTCGTCCATCCACCGGGCCTCACAAGTTGCGCGAATCCCTGCCTTTAGTGATTTTCCTTCGTAAC
	CC	AABACG	AGGCTGAAGTATGCCCTTACAAACAGTGAAGTCACTAAAATTGTCATGCAAAGGTTGATCAAAGT
			TGATGGTAAAGTGAGGACTGATTCTAATTACCCTGCTGGTTTCATGGATGTCATTACTATTGAGAA
			GACTGGTGAATTTTTCCGTCTGATCTATGATGTTAAAGGAAGATTTGCTGTGCACCGTATTACAGC
			TGAAGAGGCAAAATACAAGTTGTGTAAAGTAAGGAGAGTCCAAACTGGTCCCAAAGGAATCCCAT
			TTTTGGTAACACATGATGGCAGAACCATTCGTTACCCTGACCCCAACATCAAAGTGAATGACACA
			ATTCAAATGGAAATTGCTACATCTAAAATTCTTGACTACATCAAATTTGAATCTGGCAACCTCTGC
			ATGATCACGGGGAGG

PC003	SEQ ID NO: 263	SEQ ID NO: 264	SEQ ID NO: 249
	TCGGTCTTCTC	CCCTGGTTCTT	CCCTAGACGTCCCTATGAAAAGGCCCGTCTGGATCAGGAATTGAAAATTATCGGCGCCTTTGGTT
	GAAGACNTAYG	CTTVRRRTTCT	TACGAAACAAACGTGAAGTGTGGAGAGTAAAGTACACTTTGGCTAAAATCCGTAAAGCTGCTCGT
	TKAC	TCCTC	GAACTGCTCACCCTAGAAGAAAAAGAGCCTAAAAGATTGTTTGAAGGTAATGCACTTCTACGTCG
			TTTGGTGCGAATTGGTGTTCTGGATGAGAACAGGATGAAGCTTGATTATGTTTTGGGTCTGAAAA
			TTGAAGATTTCTTGGAAAGAAGGCTCCAAACTCAGGTGTTCAAATCTGGTCTGGCAAAGTCAATT
			CATCATGCTAGAGTACTGATTAGGCAGAGACACATCCGGGTGCGCAAGCAGGTGGTGAACATCC
			CCTCGTTCATCGTGCGGCTGGACTCGCAGAAGCACATCGACTTCTCCCTGAAGTCGCCCTTCGG
			GGGTGGCCGACCTGGCCGTGTCAA

PC005	SEQ ID NO: 265	SEQ ID NO: 266	SEQ ID NO: 251
	TGCGATGCGG	TCCTGCTTCTT	TGCGATGCGGCAAAAAGAAGGTGTGGTTGGATCCAAATGAAATCAACGAAATCGCCAACACCAA
	CAARAARAAGG	SGYRGCRATW	CTCAAGACAAAACATCCGTAAGCTCATCAAGGATGGTCTTATCATCAAGAAGCCAGTGGCAGTAC
	TBTGG	CGYTC	ACTCTAGGGCCCGTGTACGCAAGAACACTGAAGCCAGAAGGAAGGGAAGGCATTGTGGATTTG
			GAAAGAGGAAGGGTACGGCAAATGCCCGTATGCCTCAAAAGGAACTGTGGGTGCAGCGCATGC
			GCGTCCTCAGGCGCCTCCTCAAAAAGTACAGGGAGGCCAAGAAAATCGACCGCCATCTTTACCA
			CGCCCTGTACATGAAAGCGAAGGGTAACGTGTTCAGGAACAAGAGGGTCCTTATGGAGTACATC
			CACAAGAAGAAGGCAGAGAAGGCCAGGGCCAAGATGCTGTCTGACCAGGCTAACGCCAGGAGA
			TTGAAGGTGAAGCAGGCCAGGGAACGTAGGGAAGAGCGTATCGCCACCAAGAAGCAGG

PC010	SEQ ID NO: 267	SEQ ID NO: 268	SEQ ID NO: 253
	CTCTCAAGGAT	CGCCATTGGG	CTCTCAAGGATTCTTTGCAGATGTCGCTCAGCCTATTACCGCCCAACGCGTTGATTGGATTGATC
	TCKYTRCARAT	CRATGGTYTCK	ACGTTCGGAAAAAATGGTGCAAGTCCACGAACTGGGTACCGAAGGCTGCAGCAAGTCGTACGTGT
	GTC	CC	TCTGTGGAACGAAAGATCTCACCGCCAAGCAAGTCCAGGAGATGTTGGGCATTGGAAAAGGGTC
			ACCAAATCCCCAACAACAGCCAGGGCAACCTGGGCGGCCAGGGCAGAATCCCCAAGCTGCCCC
			TGTACCACCGGGGAGCAGATTCTTGCAGCCCGTGTCAAAATGCGACATGAACTTGACAGATCTG
			ATCGGGGAGTTGCAGAAAGACCCTTGGCCCGTACATCAGGGCAAAAGACCTCTTAGATCCACAG
			GCGCAGCATTGTCCATCGCTGTCGGCCTCTTAGAATGCACCTATCCGAATACGGGTGGCAGAAT
			CATGATATTCTTAGGAGGACCATGCTCTCAGGGTCCCGGCCAGGTGTTGAACGACGATTTGAAG
			CAGCCCATCAGGTCCCATCATGACATACACAAAGACAATGCCAAGTACATGAAGAAGGCTATCAA
			ACATTACGATCACTTGGCAATGCGAGCTGCCACCAACAGCCATTGCATCGACATTTACTCCTGCG
			CCCTGGATCAGACGGGACTGATGGAGATGAAGCAGTGCTGCAATTCCACCGGAGGGCACATGG
			TCATGGGCGATTCCTTCAATTCCTCTCTATTCAAACAAACCTTCCAGCGAGTGTTCTCAAAAGACC
			CGAAGAACGACCTCAAGATGGCGTTCAACGCCACCTTGGAGGTGAAGTGTTCCAGGGAGTTAAA
			AGTCCAAGGGGGCATCGGCTCGTGCGTGTCCTTGAACGTTAAAAGCCCTCTGGTTTCCGATACG
			GAACTAGGCATGGGGAATACTGTGCAGTGGAAACTTTGCACGTTGGCGCCGAGCTCTACTGTGG
			CGCTGTTCTTCGAGGTGGTTAACCAGCATTCGGCGCCCATACCACAGGGAGGCAGGGGCTGCA
			TCCAGCTCATCACCCAGTATCAGCACGCGAGCGGGCAAAGGAGGATCAGAGTGACCACGATTG
			CTAGAAATTGGGCGGACGCTACTGCCAACATCCACCACATTAGCGCTGGCTTCGACCAAGAAGC
			GGCGGCAGTTGTGATGGCCCGAATGGCCGGTTACAAGGCGGAATCGGACGAGACTCCCGACGT
			GCTCAGATGGGTGGACAGGATGTTGATCAGGCTGTGCCAGAAGTTCGGAGAGTACAATAAAGAC
			GATCCGAATTCGTTCAGGTTGGGGGAGAACTTCAGTCTGTATCCGCAGTTCATGTACCATTTGAG
			ACGGTCGCAGTTTCTGCAGGTGTTCAATAATTCTCCTGATGAAACGTCGTTTTATAGGCACATGC
			TGATGCGTGAGGATTTGACTCAGTCTTTGATCATGATCCAGCCGATTTTGTACAGTTACAGCTTCA
			ACGGGCCGCCCGAGCCTGTGTTGTTGGACACAAGCTCTATTCAGCCGGATAGAATCCTGCTCAT
			GGACACTTTCTTCCAGATACTCATTTTCCATGGAGAGACCATTGCCCAATGGCG

PC014	SEQ ID NO: 269	SEQ ID NO: 270	SEQ ID NO: 255
	CGCAGATCAAR	CGGATCTCGG	CTGATGTTCAAAAACAAATCAAACACATGATGGCTTTCATTGAACAAGAAGCCAATGAGAAAGCA
	CAYATGATGGC	GCASMARYTGC	GAAGAAATTGATGCCAAGGCAGAGGAGGAATTCAACATTGAAAAAGGGCGTTTGGTCCAGCAAC
			AGAGACTCAAGATCATGGAGTACTACGAGAAAAAGGAGAAGCAAGTCGAACTTCAAAAGAAAATT
			CAGTCCTCTAATATGTTGAATCAGGCTCGTTTGAAGGTGCTGAAAGTGAGAGAGGACCATGTCAG
			AGCAGTCCTGGAGGATGCTCGTAAAAGTCTTGGTGAAGTAACCAAAGACCAAGGAAAATACTCC
			CAAATTTTGGAGAGCCTAATCCTACAAGGACTGTTCCAGCTGTTCGAGAAGGAGGTGACGGTCC
			GCGTGAGACCGCAAGACAGGGACCTGGTCAGGTCCATCCTGCCCAACGTCGCTGCCAAATACA
			AGGACGCCACCGGCAAAGACATCCTACTCAAGGTGGACGATGAGTCGCACCTGTCTCAGGAGAT
			CACCGGAGGCGTCGATTTGCTCGCTCAGAAGAACAAGATCAAGATCAGCAACACGATGGAGGCT
			AGGTTGGATCTGATCGCTCA

PC016	SEQ ID NO: 271	SEQ ID NO: 272	SEQ ID NO: 257
	GACTGTGTCTG	GGAATAGGAT	GGAATAGGATGGGTGATGTCGTCGTTGGGCATAGTCAAGATGGGGATCTGCGTGATGGAGCCG
	GTGTRAACGG	GGGTRATRTC	TTGCGGCCCTCCACACGACCGGCGCGCTCGTAAATGGTGGCCAGATCGGTGTACATGTAACCG
	WCC	GTCG	GGGAAACCCCTACGGCCGGGCACTTCTTCTCGAGCGGCAGACACCTCACGCAACGCCTCCGCG
			TACGACGACATGTCGGTCAAGATGACCAGCACGTGCTTCTCGCACTGGTAGGCCAAGAATTCGG
			CGGCCGTCAGAGCCAAACGCGGCGTGATGATGCGCTCGATGGTCGGATCGTTGGCCAAGTTCA
			AGAACAGACACACGTTCTCCATCGAGCCGTTCTCTTCGAAGTCCTGCTTGAAGAACCTGGCAGTT
			TCCATGTTGACACCCATAGCAGCAAACACAATAGCAAAGTTGTCTTCATGGTCATCCAGCACAGA
			CTTGCCAGGTACTTTGACCAAGCCAGCCTGCCTACAAATCTGGGCTGCAATCTCATTGTGGGGC
			AGCCCAGCGGCGGAGAAGATCGGAATCTTCTGCCCTCTGGCGATAGAGTTCATCACGTCGATGG
			CCGTGATCCCAGTCTGGATCATTTCCTCGGGATAAATACGCGACCACGGGTTGATCGGCTGTCC
			TTGGATGTCGAGGTAGTCCTCAGCCAGGATCGGGGGACCTTTATCAATGGGTTTTCCTGATCCAT
			TGAAGACACGTCCCAGCATATCTTCTGATACTGGAGTTCTTAGAATATCTCCAGTGAACTCACAC
			ACCGTGTTCTTAGCATCAATACCTGATGTGCCTTCAAATACCTGAACAACTGCCTTTGATCCACTG
			ACTTCCAAAACTTGTCCAGATCGTAGAGTTCCATCTGCCAATTTGAGCTGGACAATTTCATTGAAT
			TTTGGAAACTTGACATCCTCAAGAATGACCAGTGGTCCGTTCACACCAGACACAGTC

PC027	SEQ ID NO: 273	SEQ ID NO: 274	SEQ ID NO: 259
	GGGCCAAGCA	TGTGCCACCC	GGGCCAAGCACAGTGAAATACAGCAAGCTAACTTGAAAGCACTACCAGAAGGAGCTGAAATCAG
	CWSYGAAATRC	TAGTRCGRTG	AGATGGAGAACGTTTGCCAGTCACAGTAAAGGACATGGGAGCATGCGAGATTTACCCACAAACA
	AG	YTC	ATCCAACACAACCCCAATGGGCGGTTTGTAGTGGTTTGTGGTGATGGAGAATACATAATATACAC
			GGCTATGGCCCTTCGTAACAAAGCATTTGGTAGCGCTCAAGAATTTGTATGGGCACAGGACTCC
			AGTGAATATGCCATCCGCGAATCCGGATCCACCATTCGAATCTTCAAGAATTTCAAAGAAAAAAA
			GAATTTCAAGTCCGACTTTGGTGCCGAAGGAATCTATGGTGGTTTTCTCTTGGGTGTGAAATCAG
			TGTCTGGCTTAGCTTTCTATGACTGGGAAACGCTTGAGTTAGTAAGGCGCATTGAAATACAGCCT
			AGAGCTATCTACTGGTCAGATAGTGGCAAGTTGGTATGCCTTGCTACCGAAGATAGCTATTTCAT
			ATTGTCCTATGACTCTGACCAAGTCCAGAAAGCTAGAGATAACAACCAAGTTGCCGAAGATGGAG
			TGGAGGCTGCCTTTGATGTCCTAGGTGAAATAAATGAATCCGTAAGAACAGGTCTTTGGGTAGGA
			GACTGCTTCATTTACACAAACGCAGTCAACCGTATCAACTACTTTGTGGGTGGTGAATTGGTAAC
			TATTGCACATCTGGACCGTCCTCTATATGTCCTGGGCTATGTACCTAGAGATGACAGGTTATACT
			TGGTTGATAAAGAGTTAGGAGTAGTCAGCTATCAATTGCTATTATCTGTACTCGAATATCAGACTG
			CAGTCATGCGACGAGACTTCCCAACGGCTGATCGAGTATTGCCTTCAATTCCAAAAGAACATCGC
			ACTAGGGTGGCACA

TABLE 2-EV

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

EV005	SEQ ID NO: 523	SEQ ID NO: 524	SEQ ID NO: 513
	TGCGATGCGG	TCCTGCTTCTT	TGCGATGCGGCAAGAAGAAGGTTTGGCTGGATCCTAATGAAATAACTGAAATTGCTAATACA
	CAARAARAAGG	SGYRGCRATW	AACTCTAGACAAAACATCCGCAAACTGATTAAAGATGGTCTTATTATTAAAAAGCCTGTCGCG
	TBTGG	CGYTC	GTGCATTCTCGTGCACGTGTACGCAAAAATACTGAAGCCCGCAGGAAAGGTCGTCATTGTG
			GATTTGGTAAAAGGAAAGGAACTGCAAATGCTAGGATGCCCAGAAAGGAATTATGGATTCAA
			CGTATGAGAGTTCTCAGAAGGTTATTGAAGAAATATAGGGAAGCTAAGAAAATTGATAGGCA
			TTTATACCATGCTTTATATATGAAAGCTAAGGGAAATGTATTCAAGAATAAGAGAGTAATGAT
			GGACTATATCCATAAAAAGAAGGCGGAGAAAGCACGTACAAAGATGCTCAATGATCAAGCT
			GATGCAAGGAGGCTGAAAGTCAAAGAGGCACGTAAGCGACGTGAAGAGCGTATCGCTACG
			AAGAAGCAGGA

EV009	SEQ ID NO: 525	SEQ ID NO: 526	SEQ ID NO: 515
	GGGCCGTGGT	GCAGCCCACG	CCAACTCTCGATCCAAGCATTCCAAAATACAGGACTGAAGAATCTATAATAGGAACAAACCC
	CAGAAYATYWA	CYYTGCACTC	AGGAATGGGTTTTAGGCCAATGCCCGACAACAACGAAGAAAGTACCCTGATTTGGTTACAG
	YAAC		GGTTCTAATAAAACAAACTACGAAAAATGGAAAATGAATCTCCTCTCATATTTAGACAAGTAT
			TACACTCCCGGAAAAATAGAAAAGGGAAATATTCCAGTAAAGCGCTGTTCATACGGAGAAAA
			ATTGATTAGGGGACAAGTATGTGATGTAGATGTGAGGAAATGGGAGCCGTGCACCCCGGAA
			AATCATTTTGATTACCTCAGAAATGCGCCTTGTATATTTCTGAAGCTGAACAGGATATATGGA
			TGGGAACCGGAGTACTACAACGATCCAAATGATCTTCCAGATGATATGCCGCAGCAGTTGA
			AGGACCATATACGTTATAATATCACCAATCCAGTGGAGAGAAATACCGTCTGGGTAACATGC
			GCAGGTGAAAATCCGGCAGACGTGGAGTACTTGGGCCCTGTGAAGTATTACCCATCTTTCC
			AGGGATTCCCCGGTTACTATTTTCCATATTTGAATTCTGAAGGGTACCTAAGTCCATTATTGG
			CGGTACAATTCAAGAGACCGGTGTCTGGTATTGTTATAAATATCGAGTGCAAAGCGTGGGCT
			GC

EV010	SEQ ID NO: 527	SEQ ID NO: 528	SEQ ID NO: 517
	CGGCTGACGT	CGGCGTATTCT	CTGGCGGCCACATGGTCATGGGTGATTCATTTAACTCTTCACTTTTCAAACAAACATTTCAAC
	GGAAYGTKTGG	CCRAAYTTCTG	GAGTATTTTCGAAAGATTCCAATGGAGACTTGAAGATGTCCTTCAACGCCATATTAGAAGTG
	CC	GC	AAGTGTTCTAGAGAACTTAAAGTACAAGGAGGTATAGGTCCTTGTGTCTCTCTAAATGTCAA
			AAATCCTCTTGTTTCTGATTTAGAAATAGGCATGGGTAACACAGTTCAGTGGAAACTGTGTA
			GCTTAAGTCCAAGCACTACGGTTGCCTTATTTTTCGAAGTTGTTAATCAGCATGCAGCACCC
			ATTCCTCAAGGGGGACGTGGATGCATTCAGTTTATTACTCAATATCAGCATTCAAGTGGTCA
			GAAAAAAATAAGGGTAACTACAATAGCAAGAAATTGGGCGGATGCCACTGCAAATATTCACC
			ATATTAGCGCTGGCTTTGACGAACAAACTGCGGCTGTTTTAATGGCGAGGATCGCTGTATAT
			AGAGCAGAAACTGATGAGAGTTCAGATGTTCTCAGATGGGTTGACAGAATGTTGATACGATT
			GTGTCAGAAATTTGGAGAATATAACAAAGATGACACCAACAGCTTCAGGCTCAGTGAAAAACT
			TCAGCTTATATCCACAGTTTATGTATCATCTACGTCGTTCCCAATTTCTACAAGTGTTCAATAA
			TTCACCAGATGAAACTTCATTCTATAGGCACATGTTGATGAGGGAAGATCGCAATCAG

EV015	SEQ ID NO: 529	SEQ ID NO: 530	SEQ ID NO: 519
	CGCTGTCGCAR	CGATCAAAGC	CGCCATCCGTCGCTGTTCAAGGCGATCGGCGTTAAGCCTCCAAGGGGTATTCTCCTTTACG
	GCRAARATGG	GWCCRAAVCG	GGCCTCCCGGCACGGGGAAAACGCTGATCGCCAGGGCCGTTGCCAACGAAACTGGTGCGT
		ACG	TCTTCTTCCTCATCAATGGGCCCGAGATTATGAGCAAGCTGGCCGGAGAATCCGAGAGCAA
			TCTTAGAAAGGCTTTTGAAGAGGCTGATAAAAACTCTCCTGCAATCATCTTTATCGACGAATT
			AGACGCAATCGCTCCCAAGCGCGAGAAGACTCATGGTGAGGTAGAGAGACGCATCGTCTC
			CCAACTGTTGACTTTGATGGACGGCATGAAGAAAAGTTCCCATGTGATCGTGATGGCGGCC
			ACGAACAGGCCCAATTCCATCGACCCTGCACTCAGACGTTTCGGCCGATTCGACAGAGAGA
			TCGACATCGGTATCCCCGACGCTACTGGAAGATTAGAAGTACTCAGAATACACACCAAAAAC
			ATGAAATTGGCTGACGATGTAGATTTGGAACAGATTGCCGCAGAGACTCACGGTCATGTAG
			GTGCTGACTTGGCTTCTTTGTGCTCAGAGGCTGCCTTGCAACAAATTAGAGAAAAAATGGAC
			CTCATCGACTTAGATGATGAGCAGATCGATGCCGAAGTCCTAAATTCTCTGGCAGTTACCAT
			GGAGAACTTCCGTTACGCCATGTCTAAGAGCAGTCCGAGCGCTTTGCGCGAAACCGTCGT

EV016	SEQ ID NO: 531	SEQ ID NO: 532	SEQ ID NO: 521
	GTTCACCGGC	CGGCATAGTC	GACTGTGTCTGGTGTGAACGGACCGTTGGTGATCCTTGATAGTGTTAAGTTTCCAAAATTTA
	GAYATYCTGCG	AGAATSGGRAT	ACGAAATTGTACAGCTCAAGTTATCAGATGGAACAGTTAGGTCTGGACAAGTTTTGGAAGTC
		CTG	AGTGGACAGAAGGCGGTTGTCCAAGTTTTTGAAGGCACCTCCGGAATTGATGCTAAAAACA
			CTTTATGTGAATTTACAGGAGATATCTTAAGAACTCCAGTGTCTGAAGATATGTTGGGTCGT
			GTGTTTAATGGATCTGGAAAGCCTATCGATAAAGGGCCGCCAATCTTAGCTGAAGATTTTCT
			TGACATTCAAGGTCAACCTATAAATCCTTGGTCTCGTATCTATCCAGAAGAAATGATCCAGA
			CTGGTATTTCTGCGATTGATGTGATGAATTCCATTGCCAGAGGACAAAAGATTCCAATTTTCT
			CTGCAGCTGGTTTACCCCACAATGAAATCGCTGCTCAAATCTGTAGACAAGCTGGTCTTGTC
			AAAATCCCAGGGAAATCTGTCTTAGATGATCATGAAGACAACTTTGCTATCGTTTTCGCCGC
			TATGGGTGTCAATATGGAAACAGCCAGATTCTTCAAGCAAGATTTTGAAGAGAATGGCTCTA
			TGGAAAATGTGTGCCTATTTTTGAACTTGGCCAATGATCCTACCATTGAAAGAATTATAACAC
			CCCGTTTGACTTTAACAGCGGCTGAATTTATGGCATATCAATGTGAGAAGCATGTGTTAGTC
			ATATTGACTGACATGTCATCTTATGCTGAGGCTTTGCGTGAGGTATCTGCTGCT

TABLE 2-AG

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

AG001	SEQ ID NO: 611	SEQ ID NO: 612	SEQ ID NO: 601
	CATTTGAAGCG	CGCTTGTCCC	CATTTGAAGCGTTTTGCTGCCCCCAAAGCATGGATGTTGGACAAATTGGGGGGTGTGTTCGCCC
	TTTWRMYGCYCC	GCTCCTCNGC	CCAGGCCCTCCACCGGGCCACACAAGCTCAGGGAGTCCCTTCCATTAGTGATTTTCTTGCGTAA
		RAT	CAGGTTGAAGTACGCCCTGACAAACTGTGAGGTGACCAAGATCGTTATGCAGAGACTTATTAAG
			GTCGACGGCAAAGTCAGGACTGATCCTAACTATCCTGCTGGATTCATGGATGTGATCACCATTGA
			AAAAACTGGTGAATTCTTCCGTTTGATCTATGATGTTAAGGGAAGATTCACTATTCACAGGATCAC
			TGCTGAAGAAGCAAAATACAAATTGTGCAAAGTCCGCAAGGTGCAAACCGGACCAAAAGGTATTC
			CATTCTTGGTCACCCACGATGGTAGGACCATTAGGTACCCTGACCCAATGATCAAGGTAAACGAC
			ACCATCCAACTGGAAATCGCCACCTCAAAGATCCTGGACTTTATCAAATTCGAATCCGGCAACTT
			GTGCATGATCACCGGAGGCAGGAATTTGGGTAGAGTGGGAACGGTAGTGAACAGGGAAAGGCA
			TCCGGGATCATTCGATATTGTCCACATTAGGGACGCTAATGATCACGTGTTCGCCACTAGATTAA
			ACAACGTATTCGTCATCGGTAAAGGAAGCAAAGCTTTCGTGTCTCTGCCAAGGGGCAAGGGAGT
			GAAACTGTCCATCGCTG

AG005	SEQ ID NO: 613	SEQ ID NO: 614	SEQ ID NO: 603
	GGTCTGGTTGG	TCCTGCTTCTT	GGTCTGGTTGGATCCAAATGAAATCAATGAGATTGCCAACACCAACTCGAGGCAAAACATCCGTA
	ATCCHAATGAA	SGYRGCRATW	AATTGATCAAGGATGGTTTGATCATTAAGAAACCGGTGGCAGTGCACTCTAGGGCTCGTGTCCGT
	ATCAAYGA	CGYTC	AAAAACACAGAAGCTCGCAGGAAGGGAAGGCACTGCGGTTTCGGTAAGAGGAAAGGTACAGCG
			AACGCTCGTATGCCTCAAAAGGAACTATGGATCCAAAGGATGCGTGTCTTGAGGCGTCTCCTGA
			AAAAATACAGGGAAGCCAAAAAGATCGACAGGCATCTGTACCACGCCCTGTACATGAAGGCCAA
			GGGTAACGTGTTCAAGAACAAGAGAGTGTTGATGGAATACATCCACAAGAAGAAGGCTGAGAAG
			GCCCGTGCCAAGATGTTGGCCGACCAAGCTAACGCCAGAAGGCAAAAGGTGAAACAAGTCCCG
			TGAGAGGAGGGAAGAGCGTATCGCCGCGAAGAAGCAGGA

AG010	SEQ ID NO: 615	SEQ ID NO: 616	SEQ ID NO: 605
	CTGGCGGCCA	CGCCATTGGG	CTGGCGGCCACATGCTTATGGGAGACTCTTTCAATTCGTCGTTGTTCAAACAAACTTTCCAAAGG
	CATGSTBATGG	CRATGGTYTCK	GTGTTCGCGAAGGACCAGAATGGACATTTGAAGATGGCTTTCAACGGTACTTTGGAGGTGAAGT
		CC	GCTCTAGGGAATTAAAAGTTCAAGGCGGTATTGGCTCATGCGTGTCGCTAAATGTAAAAAGTCCT
			TTGGTAGCGGACACGGAAATAGGCATGGGAAACACCGTGCAATGGAAGATGTGCACCTTCAACC
			CTAGCACGACGATGGCGCTGTTTTTCGAGGTGGTCAATCAGCATTCGGCCCCCATTCCTCAAGG
			TGGTAGAGGATGTATACAGTTTATTACACAATATCAGCACTCGAGTGGCCAAAGGAGGATAAGGG
			TGACGACGATAGCGAGAAATTGGGCGGACGCATCGGCGAATATTCACCACATCAGCGCGGGTTT
			CGATCAGGAACGTGCCGCGGTGATTATGGCCCGGATGGCTGTTTATAGAGCGGAGACCGATGA
			GAGTCCCGATGTTTTAAGATGGGTCGATCGGATGCTGATTCGTTTGTGTCAAAAGTTTGGAGAAT
			ATAACAAAGATGACCAGGCATCCTTCAGATTAGGAGAAAATTTTAGCTTATACCCGCAATTCATGT
			ACCACTTAAGGCGATCCCAGTTTTTGCAAGTGTTCAACAATTCACCTGACGAAACGTCGTTTTACA
			GGCATATGCTTATGAGGGAAGATTTGACACAGTCCCTGATAATGATTCAGCCGATCTTGTACAGT
			TACAGTTTTAATGGTCCTCCGGAGCCCGTTTTGTTGGACACCAGCTCAATACAACCGGACAGAAT
			TCTGCTTATGGACACGTTTTTCCAGATATTGATTTTCCATGGAGAAACCATTGCCCAATGGCG

AG014	SEQ ID NO: 617	SEQ ID NO: 618	SEQ ID NO: 607
	CGCAGATCAAR	GAACTTGCGG	CGCAGATCAAGCATATGATGGCCTTCATTGAGCAAGAGGCTAATGAAAAGGCCGAGGAAATTGA
	CAYATGATGGC	TTGABGTTSCG	TGCCAAGGCGGAAGAAGAATTTAACATTGAAAAGGGCCGCCTTGTGCAACAACAAAGATTGAAG
		DCC	ATCATGGAATACTATGAGAAGAAGGAGAAGCAAGTCGAACTACAAAAGAAAATTCAATCCTCCAA
			CATGCTGAACCAAGCCCGTCTTAAGGTTCTGAAAGTCCGCGAAGATCATGTTAGAGCTGTATTGG
			ATGAGGCTCGCAAGAAGCTTGGTGAAGTCACCAGGGATCAAGGCAAATATGCCCAGATTCTGGA
			ATCTTTGATCCTTCAGGGACTCTACCAGCTTTTCGAGGCAAACGTGACCGTACGCGTCCGCCCA
			CAAGACAGAACCTTAGTCCAATCAGTGCTGCCAACCATCGCAACCAAATACCGTGACGTCACCG
			GCCGAGATGTACACCTGTCCATCGATGACGAAACTCAACTGTCCGAATCCGTAACCGGCGGAAT
			CGAACTTTTGTGCAAACAAAACAAAATTAAGGTCTGCAACACCCTGGAGGCACGTTTGGACCTGA
			TTTCGCAACAGTTGGTTCCGCAAATCCGTAACGCCTTGTTCGGACGCAACATCAACCGCAAGTTC

AG016	SEQ ID NO: 619	SEQ ID NO: 620	SEQ ID NO: 609
	GTGTCGGAGG	GGAATAGGAT	GTGTCGGAGGATATGTTGGGCCGAGTGTTCAACGGATCAGGAAAACCCATTGACAAAGGTCCTC
	ATATGYTGGGY	GGGTRATRTC	CAATCTTAGCCGAAGATTTCTTGGACATCCAAGGTCAACCCATCAACCCATGGTCGCGTATCTAC
	CG	GTCG	CCGGAAGAAATGATCCAGACCGGTATCTCCGCCATCGACGTGATGAACTCCATCGCGCGTGGG
			CAAAAAATCCCCATTTTCTCCGCGGCCGGTTTACCGCACAACGAAATCGCCGCCCAAATCTGTAG
			ACAGGCCGGTTTAGTCAAACTGCCGGGCAAATCGGTAATCGACGATCACGAGGACAATTTCGCC
			ATCGTGTTCGCCGCCATGGGTGTCAACATGGAAACCGCCCGTTTCTTCAAGCAGGACTTCGAAG
			AAAACGGTTCCATGGAGAACGTGTGTCTCTTCTTGAATTTGGCCAACGATCCCACCATCGAGAGA
			ATCATCACGCCCCGTTTGGCTCTGACCGCCGCCGAATTTTTGGCTTATCAATGCGAGAAACACGT
			GCTGGTTATCTTAACTGATATGTCTTCTTACGCCGAGGCTTTGCGTGAAGTATCCGCCGCCAGAG
			AAGAAGTACCCGGACGTCGTGGGTTCCCCGGTTACATGTACACCGATTTGGCCACCATTTACGA
			AAGAGCCGGTCGCGTTGAGGGTAGAAACGGTTCCATCACCCAGATTCCCATCTTGACTATGCCG
			AACGACGACATCACCCATCCTATTCC

TABLE 2-TC

	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
Target ID	5′ → 3′	5′ → 3′	5′ → 3′

TC001	SEQ ID NO: 803	SEQ ID NO: 804	SEQ ID NO: 793
	GGCCCCAAGA	CGCTTGTCCC	GGCCCCAAGAAGCATTTGAAGCGTCTCAATGCGCCCAAAGCATGGATGTTGGATAAACTG
	AGCATTTGAAG	GCTCCTCNGC	GGGGGTGTGTTTGCCCCTCGGCCTTCCACCGGCCCCCACAAGCTACGGGAGTCGCTACC
	CG	RAT	TTTGGTTATCTTCCTGCGAAACAGGCTGAAGTATGCCTTGACCAACTCAGAAGTGACGAA
			GATTGTTATGCAAAGATTGATTAAAGTTGACGGAAAAGTTAGGACAGACCCCAACTACCCC
			GCGGGTTTCATGGATGTTGTGACTATTGAGAAAACTGGGGAATTCTTCCGCTTGATTTATG
			ATGTTAAGGGAAGGTTCACAATCCATCGCATTACTGGAGAAGAGGCCAAATATAAATTGTG
			CAAAGTGAAGAAAGTACAGACAGGCCCCAAGGGCATTCCCTTCTTGGTGACCCGCGACG
			GACGCACTATCAGATACCCAGACCCCATGATCAAAGTGAATGACACCATTCAATTGGAGAT
			TGCCACTTCGAAAATTCTTGATTTTATCAAATTTGAGTCCGGTAATTTGTGTATGATTACTG
			GAGGTCGTAACTTGGGGCGTGTCGGTACAGTGGTGAGCCGAGAACGTCACCCAGGTTCC
			TTCGACATCGTTCATATTAAGGATGCAAATGGGCACACC

TC002	SEQ ID NO: 805	SEQ ID NO: 806	SEQ ID NO: 795
	CAGGAGTTCCT	GCAATGTCATC	CAGGAGTTCCTGGAGGCTAAAATCGACCAAGAGATCCTCACAGCGAAGAAAAACGCGTC
	GGARRMBAAR	CATCAKRTCRT	GAAAAACAAACGAGCGGCCATCCAGGCCATCAAGAGGAAGAAACGCTACGAAAAGCAGC
	ATMGA	GTAC	TCCAGCAGATCGATGGCACCCTCAGCACCATCGAGATGCAGCGGGAGGCCCTCGAGGG
			GGCCAACACCAACACAGCCGTACTCAAAACGATGAAAAACGCAGCGGACGCCCTCAAAAA
			TGCCCACCTCAACATGGATGTTGATGAGGTACATGACATGATGGATGACATTGC

TC010	SEQ ID NO: 807	SEQ ID NO: 808	SEQ ID NO: 797
	GCATTCTGCGC	TGCCGGAAGT	AAAATTCGGCGAATACAACAAAGACGACCCTAACAGTTTCCGTTTGAGTGAAAACTTCAGT
	TGGGTCGATCG	TCTCRTAYTCK	CTCTATCCCCAATTCATGTACCATTTGCGCCGCTCCCAATTCCTCCAAGTTTTCAACAACT
		GGC	CCCCAGACGAGACCTCGTTCTACCGCCACATGCTGATGCGGGAGGACCTCACCCAAAGT
			CTCATTATGATCCAGCCGATTTTGTACAGTTATAGTTTCAACGGCCCCCCTGAACCCGTCC
			TCCTCGACACTAGTTCCATTCAACCCGATCGGATCCTTCTCATGGACACATTTTTCCAAATT
			TTGATTTTCCACGGTGAGACAATCGCCCAATGGAGGAACCTCAAGTACCAGGACATGCCC
			GAATACGAGAACTTCCGGCA

TC014	SEQ ID NO: 809	SEQ ID NO: 810	SEQ ID NO: 799
	GAGAAAGCCG	GAACTTGCGG	GAGAAAGCCGAAGAAATCGATGCGAAAGCTGAGGAGGAGTTTAACATTGAAAAAGGGCG
	ARGARATYGAT	TTGABGTTSCG	CCTGGTCCAACAACAGCGCTTGAAGATCATGGAATATTACGAGAAGAAGGAGAAACCGGT
	GC	DCC	GGAATTGCAGAAGAAAATTCAGTCGTCAAACATGCTGAACCAAGCCCGTTTGAAAGTATTA
			AAAGTGCGTGAAGACCACGTCCACAATGTGCTGGATGACGCCCGCAAACGTCTGGGCGA
			AATCACCAATGACCAGGCGAGATATTCACAACTTTTGGAGTCTCTTATCCTCCAGAGTCTC
			TACCAGTACTTGGGAATCAGTGATGAGTTGTTTGAGAACAATATAGTGGTGAGAGTCAGG
			CAACAGGACAGGAGTATAATCCAGGGCATTCTCCCAGTTGTTGCGACGAAATACAGGGAC
			GCCACTGGTAAAGACGTTCATCTTAAAATCGACGATGAGAGCCACTTGCCATCCGAAACC
			ACCGGAGGAGTGGTTTTGTATGCGCAAAAGGGTAAAATCAAGATTGACAACACCTTGGAG
			GCTCGTTTGGATTTAATTGCACAGCAACTTGTGCCAGAAATTCGTACGGCCTTGTTTGGAC
			GCAACATCAACCGCAAGTTC

TC015	SEQ ID NO: 811	SEQ ID NO: 812	SEQ ID NO: 801
	GGATGAACTAC	CGATCAAAGC	GGATGAACTACAGCTGTTCCGTGGCGATACAGTGTTGCTGAAAGGGAAGCGGCGGAAAG
	AGCTBTTCCGH	GWCCRAAVCG	AGACCGTCTGCATTGTGCTGGCCGACGAAAACTGCCCCGATGAGAAGATCCGGATGAAC
	GG	ACG	AGGATCGTCAGGAATAATCTACGGGTTAGGCTCTCTGACGTCGTCTGGATCCAGCCCTGT
			CCCGACGTCAAATACGGGAAGAGGATCCACGTTTTGCCCATCGATGACACGGTCGAAGG
			GCTCGTCGGAAATCTCTTCGAGGTGTACTTAAAACCATACTTCCTCGAAGCTTATCGACCA
			ATCCACAAAGGCGACGTTTTCATCGTCCGTGGTGGCATGCGAGCCGTTGAATTCAAAGTG
			GTGGAAACGGAACCGTCACCATATTGTATCGTCGCCCCCGATACCGTCATCCATTGTGAC
			GGCGATCCGATCAAACGAGAAGAAGAGGAGGAAGCCTTGAACGCCGTCGGCTACGACGA
			TATCGGCGGTTGTCGCAAACAACTCGCACAAATCAAAGAAATGGTCGAATTACCTCTACG
			CCACCCGTCGCTCTTCAAGGCCATTGGCGTGAAACCACCACGTGGTATCCTCTTGTACGG
			ACCTCCAGGTACCGGTAAAACTTTAATCGCACGTGCAGTGGCCAACGAAACCGGTGCTTT
			CTTCTTCTTAATCAACGGTCCCGAAATTATGAGTAAATTAGCCGGCGAATCCGAAAGTAAT
			CTAAGGAAAGCGTTCGAAGAAGCCGATAAAAACTCACCGGCTATTATTTTCATCGATGAAT
			TGGACGCGATTGCACCGAAACGTGAAAAAACCCACGGCGAAGTCGAACGCCGAATTGTC
			TCGCAATTGTTAACACTGATGGACGGCATGAAGAAAAGCTCGCATGTTATCGTGATGGCG
			GCCACAAATCGCCCGAACTCAATCGATCCGGCTTTGCGTCGGTTCGGTCGCTTTGATCG

TABLE 2-MP

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

MP001	SEQ ID NO: 898	SEQ ID NO: 899	SEQ ID NO: 888
	GGCCCCAAGAA	CGCTTGTCCC	GGCCCCAAGAAGCATTTGAAGCGTTTAAACGCACCCAAAGCATGGATGTTGGACAAATCGGG
	GCATTTGAAGCG	GCTCCTCNGC	GGGTGTCTTCGCTCCACGTCCAAGCACCGGTCCACACAAACTTCGTGAATCACTACCGTTATT
		RAT	GATCTTCTTGCGTAATCGTTTGAAGTATGCACTTACTGGTGCCGAAGTCACCAAGATTGTCAT
			GCAAAGATTAATCAAGGTTGATGGCAAAGTCCGTACCGACCCTAATTATCCAGCCGGTTTTAT
			GGATGTTATATCTATCCAAAAGACCAGTGAGCACTTTAGATTGATCTATGATGTGAAAGGTCG
			TTTCACCATCCACAGAATTACTCCTGAAGAAGCAAAATACAAGTTGTGTAAAGTAAAGAGGGT
			ACAAACTGGACCCAAAGGTGTGCCATTTTTAACTACTCATGATGGCCGTACTATTCGCTACCC
			TGACCCTAACATCAAGGTTAATGACACTATTAGATACGATATTGCATCATCTAAAATTTTGGAT
			CATATCCGTTTTGAAACTGGAAACTTGTGCATGATAACTGGAGGTCGCAATTTAGGGCGTGTT
			GGTATTGTTACCAACAGGGAAAGACATCCAGGATCTTTTGATATTGTTCACATTAAGGATGCA
			AATGAACATATTTTTGCTACCCGGATGAACAATGTTTTTATTATTGGAAAAGGTCAAAAGAACT
			ACATTTCTCTACCAAGGAGTAAGGGAGTTAAATTGACTAT

MP002	SEQ ID NO: 900	SEQ ID NO: 901	SEQ ID NO: 890
	GAGTTTCTTTA	GCAATGTCATC	GAGTTTCTTTAGTAAAGTATTCGGTGGCAAAAAGGAAGAGAAGGGACCATCAACCGAAGATG
	GTAAAGTATTC	CATCAKRTCRT	CGATACAAAAGCTTCGATCCACTGAAGAGATGCTGATAAAGAAACAAGAATTTTTAGAAAAAA
	GGTGG	GTAC	AAATTGAACAAGAAGTAGCGATAGCCAAAAAAAATGGTACAACTAATAAACGAGCTGCATTGC
			AAGCATTGAAGCGTAAGAAACGGTACGAACAACAATTAGCCCAAATTGATGGTACCATGTTAA
			CTATTGAACAACAGCGGGAGGCATTAGAAGGTGCCAACACAAATACAGCAGTATTGACTACC
			ATGAAAACTGCAGCAGATGCACTTAAATCAGCTCATCAAAACATGAATGTAGATGATGTACAT
			GATCTGATGGATGACATTGC

MP010	SEQ ID NO: 902	SEQ ID NO: 903	SEQ ID NO: 892
	GTGGCTGCATA	CGCGGCTGCT	GTGGCTGCATACAGTTCATTACGCAGTATCAACATTCCAGTGGCTATAAACGAATTAGAGTCA
	CAGTTCATTAC	CCATGAAYASY	CCACATTAGCTAGGAATTGGGCAGACCCTGTTCAGAATATGATGCATGTTAGTGCTGCATTTG
	GCAG	TG	ATCAAGAAGCATCTGCCGTTTTAATGGCTCGTATGGTAGTGAACCGTGCTGAAACTGAGGATA
			GTCCAGATGTGATGCGTTGGGCTGATCGTACGCTTATACGCTTGTGTCAAAAATTTGGTGATT
			ATCAAAAAGATGATCCAAATAGTTTCCGATTGCCAGAAAACTTCAGTTTATATCCACAGTTCAT
			GTATCATTTAAGAAGGTCTCAATTTCTACAAGTTTTTAATAATAGTCCTGATGAAACATCATATT
			ATAGGCACATGTTGATGCGTGAAGATGTTACCCAAAGTTTAATCATGATACAGCCAATTCTGT
			ATAGCTATAGTTTTAATGGTAGGCCAGAACCTGTACTTTTGGATACCAGTAGTATTCAACCTGA
			TAAAATATTATTGATGGACACATTTTTCCATATTTTGATATTCCATGGAGAGACTATTGCTCAAT
			GGAGAGCAATGGATTATCAAAATAGACCAGAGTATAGTAACCTCAAGCAGTTGCTTCAAGCCC
			CCGTTGATGATGCTCAGGAAATTCTCAAAACTCGATTCCCAATGCCTCGGTATATTGACACAG
			AACAAGGTGGTAGTCAGGCAAGATTTTTACTATGCAAAGTAAACCCATCTCAAACACATAATAA
			TATGTATGCTTATGGAGGGTGATGGTGGAGCACCAGTTTTGACAGATGATGTAAGCTTGCAG
			CTGTTCATGGAGCAGCCGCG

MP016	SEQ ID NO: 904	SEQ ID NO: 905	SEQ ID NO: 894
	GTGTCGGAGG	GGAATAGGAT	GTGTCGGAGGATATGTTGGGCCGCGTTTTCAATGGCAGTGGAAAGCCGATAGATAAAGGACC
	ATATGYTGGGY	GGGTRATRTC	TCCTATTTTGGCTGAAGATTATTTGGATATTGAAGGCCAACCTATTAATCCATACTCCAGAACA
	CG	GTCG	TATCCTCAAGAAATGATTCAAACTGGTATTTCAGCTATTGATATCATGAACTCTATTGCTCGTG
			GACAAAAAATTCCAATATTTTCAGCTGCAGGTTTACCACATAATGAGATTGCTGCTCAAATTTG
			TAGACAAGCTGGTCTCGTTAAAAAACCTGGTAAATCAGTTCTTGACGATCATGAAGACAATTTT
			GCTATAGTATTTGCTGCTATGGGTGTTAATATGGAAACAGCCAGATTCTTTAAACAAGATTTTG
			AGGAAAATGGTTCAATGGAGAATGTTTGTTTGTTCTTGAATTTAGCTAATGATCCTACTATTGA
			GCGTATCATTACACCACGTCTTGCTTTAACTGCTGCTGAATTTTTAGCTTACCAATGTGAAAAG
			CATGTCTTAGTTATTTTAACTGACATGAGTTCATATGCTGAAGCTTTAAGAGAAGTTTCTGCTG
			CTCGTGAAGAAGTACCTGGGCGTCGTGGTTTCCCTGGTTACATGTACACCGATTTAGCTACAA
			TTTATGAACGTGCTGGGCGTGTAGAAGGAAGAAATGGTTCTATCACACAAATACCTATTTTAA
			CTATGCCTAACGACGACATCACCCATCCTATTCC

MP027	SEQ ID NO: 906	SEQ ID NO: 907	SEQ ID NO: 896
	CGCCGATTACC	GGGATACTGT	CGCCGATTACCAAAACAAGACGTGTGTTCAGACATTAGAAGGCCATGCTCAAAATATTTCTGC
	AAAACAARACB	CACAAYYTCDC	TCGTTTGTTTCCATCCAGAACTTCCCATCGTGTTAACTGGCTCAGAAGATGGTACCGTCAGAA
	TG	CRCC	TTTGGCATTCTGGTACTTATCGATTAGAATCATCATTAAACTATGGGTTAGAACGTGTATGGAC
			AATCTGTTGCTTACGGGGATCTAATAATGTAGCTCTAGGTTATGATGAAGGAAGTATAATGGT
			TAAAGTTGGTCGTGAAGAGCCAGCAATGTCAATGGATGTTCATGGGGGTAAAATTGTTTGGG
			CACGTCATAGTGAAATTCAACAAGCTAACCTTAAAGCGATGCTTCAAGCAGAAGGAGCCGAAA
			TCAAAGATGGTGAACGTTTACCAATACAAGTTAAAGACATGGGTAGCTGTGAAATTTATCCAC
			AGTCAATATCTCATAATCCGAATGGTAGATTTTTAGTAGTATGTGGTGATGGAGAGTATATTAT
			ATATACATCAATGGCTTTGCGTAATAAAGCATTTGGCTCCGCTCAGGATTTTGTATGGTCTTCT
			GATTCTGAGTATGCCATTAGAGAAAATTCTTCTACAATCAAAGTTTTTAAAAATTTTAAAGAAAA
			AAAGTCTTTTAAACCAGAAGGTGGAGCAGATGGTATTTTTGGAGGTTATTTGTTAGGTGTGAA
			ATCTGTTACTGGGTTGGCTTTATATGATTGGGAAAATGGTAACTTAGTTCGAAGAATTGAGAC
			ACAACCTAAACATGTATTTTGGTCAGAGTCTGGAGAATTAGTATGTCTTGCCACAGATGAAGC
			ATACTTTATTTTACGTTTTGACGTCAATGTACTTAGTGCTGCAAGAGCATCCAATTATGAAGCT
			GCTAGTCCTGATGGTCTTGAAGATGCCTTTGAGATTTTAGGAGAAGTTCAAGAAGTTGTAAAA
			ACTGGTCTATGGGTTGGTGATTGCTTTATTTACACCAATGGAGTAAATCGTATCAACTATTATG
			TTGGTGGTGAAGTTGTGACAGTATCCC

TABLE 2-NL

	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
Target ID	5′ → 3′	5′ → 3′	5′ → 3′

NL001	SEQ ID NO: 1117	SEQ ID NO: 1118	SEQ ID NO: 1071
	GAAATCATGGAT	ACTGAGCTTCACAC	GAAATCATGGATGTTGGACAAATTGGGTGGTGTGTATGCACCCCGACCCAGCACAGG
	GTTGGACAAATT	CCTTGCCC	TCCACACAAGCTGCGAGAATCTCTCCCACTTGTCATATTTTTGCGTAATCGGCTCAAG
	GG		TACGCTTTAACTAACTGTGAAGTGAAGAAAATTGTGATGCAGCGTCTCATCAAGGTTG
			ACGGCAAAGTGAGGACTGACCCCAACTATCCTGCAGGTTTTATGGACGTTGTTCAAAT
			CGAAAAGACAAACGAGTTCTTCCGTTTGATCTATGATGTTAAGGGACGTTTCACCATC
			CACAGGATCACAGCTGAAGAAGCTAAGTACAAGCTGTGCAAAGTGAAGAGGGTTCAG
			ACAGGACCCAAGGGCATTCCATTTTTGACCACTCACGATGGACGCACCATCAGGTAT
			CCAGACCCCTTGGTAAAAGTCAATGACACCATCCAATTGGACATTGCCACATCCAAAA
			TCATGGACTTCATCAGATTCGACTCTGGTAACCTGTGTATGATCACTGGAGGTCGTAA
			CTTGGGTCGTGTGGGCACTGTCGTGAACAGGGAGCGACACCCGGGGTCTTTCGACA
			TCGTGCACATCAAGGACGTGTTGGGACACACTTTTGCCACTAGGTTGAACAACGTTTT
			CATCATCGGCAAGGGTAGTAAAGCATACGTGTCTCTGCCCAAGGGCAAGGGTGTGAA
			GCTCAGT

NL002	SEQ ID NO: 1119	SEQ ID NO: 1120	SEQ ID NO: 1073
	GATGAAAAGGG	CTGATCCACATCCA	GATGAAAAGGGCCCTACAACTGGCGAAGCCATTCAGAAACTACGCGAAACAGAGGAA
	CCCTACAACTG	TGTGTTGATGAG	ATGCTGATAAAGAAACAAGACTTTTTAGAAAAGAAAATTGAAGTTGAAATTGGAGTTGC
	GC		CAGGAAGAATGGAACAAAAAACAAAAGAGCCGCGATCCAGGCACTCAAAAGGAAGAA
			GAGGTATGAAAAGCAATTGCAGCAGATCGATGGAACGTTATCAACAATTGAGATGCA
			GAGAGAGGCCCTCGAAGGAGCCAACACGAATACGGCCGTACTGCAAACTATGAAGA
			ACGCAGCAGATGCTCTCAAAGCGGCTCATCAACACATGGATGTGGATCAG

NL003	SEQ ID NO: 1121	SEQ ID NO: 1122	SEQ ID NO: 1075
	TCCGCGTCGTC	TTGACGCGACCAG	TCCGCGTCGTCCTTACGAGAAGGCACGTCTCGAACAGGAGTTGAAGATCATCGGAGA
	CTTACGAGAAG	GTCGGCCAC	GTATGGACTCCGTAACAAGCGTGAGGTGTGGAGAGTCAAATACGCCCTGGCCAAGAT
	GC		TCGTAAGGCCGCTCGTGAGCTGTTGACTCTGGAAGAGAAGGACCAGAAACGTTTGTT
			TGAAGGTAACGCCCTGCTGCGTCGCCTGGTGCGTATTGGAGTGTTGGACGAAGGAA
			GAATGAAGCTCGATTACGTCTTGGGTTTAAAAATTGAAGATTTCCTTGAACGTCGTCT
			ACAGACTCAGGTGTACAAACTCGGTTTGGCCAAGTCCATCCATCACGCCCGTGTACT
			CATCAGACAAAGACATATCAGAGTGCGCAAACAAGTAGTGAACATTCCGAGCTTTGTG
			GTGCGCCTGGACTCGCAGAAGCACATTGACTTCTCGCTGAAGTCGCCGTTCGGCGG
			TGGCCGACCTGGTCGCGTCAA

NL004	SEQ ID NO: 1123	SEQ ID NO: 1124	SEQ ID NO: 1077
	TGAAGGTGGAG	GTCGTCTTCTCDGA	AAGGAGTTGGCTGCTGTAAGAACTGTCTGCTCTCACATCGAAAACATGCTGAAGGGA
	AARGGTTYGGM	HACRTAVAGACC	GTCACAAAGGGATTCCTGTACAAGATGCGTGCCGTGTACGCCCATTTCCCCATCAAC
	WCMAAG		TGTGTGACGACCGAGAACAACTCTGTGATCGAGGTGCGTAACTTCCTGGGCGAGAAG
			TACATCCGACGGGTGAGGATGGCGCCCGGCGTCACTGTTACCAACTCGACAAAGCA
			GAAGGACGAGCTCATCGTCGAAGGAAACAGCATAGAGGACGTGTCAAGATCAGCTG
			CCCTCATCCAACAGTCAACAACAGTGAAGAACAAGGATATTCGTAAATTCTTGGAC

NL005	SEQ ID NO: 1125	SEQ ID NO: 1126	SEQ ID NO: 1079
	GGTCTGGTTGG	TCCTGCTTCTTSGY	TTGGATCCCAATGAAATAAATGAAATCGCAAACACAAATTCACGTCAAAGCATCAGGA
	ATCCHAATGAAA	RGCRATWCGYTC	AGCTGATCAAAGACGGTCTTATCATCAAGAAACCGGTTGCAGTACATTCACGTGCTCG
	TCAAYGA		CGTTCGTAAAAACACTGAAGCCAGGAGGAAAGGCAGACATTGTGGCTTTGGTAAGAG
			GAAAGGTACAGCCAACGCCCGTATGCCACAAAAGGTTCTATGGGTGAATCGTATGCG
			TGTCTTGAGAAGACTGTTGAAAAAATACAGACAAGATAAGAAAATCGACAGGCATCTG
			TACCATCACCTTTACATGAAGGCTAAGGGTAACGTATTCAAGAACAAGCGTGTATTGA
			TGGAGTTCATTCATAAGAAGAAGGCCGAGAAAGCAAGAATGAAGATGTTGAACGACC
			AGGCTGAAGCTCGCAGACAAAAGGTCAAGGAGGCCAAGAAGCGAAGGGAA

NL006	SEQ ID NO: 1127	SEQ ID NO: 1128	SEQ ID NO: 1081
	GGAGCGAGACT	GAGATCTTCTGCAC	AAGTGCTTGTGTCAAGTGGTGTGGTGGAGTACATTGACACCCTGGAGGAGGAGACG
	ACAACAAYKAYR	RTTKACVGCATC	ACCATGATAGCGATGTCGCCGGATGACCTGCGTCAGGACAAGGAGTATGCCTACTGT
	GYTGGC		ACCACCTACACGCACTGCGAGATCCACCCGGCCATGATACTCGGTGTGTGCGCCTCT
			ATTATTCCCTTCCCCGATCACAACCAAAGTCCCAGGAACACCTATCAGAGCGCTATGG
			GGAAACAGGCGATGGGCGTGTACATCACCAACTTCCACGTGCGAATGGACACGCTG
			GCTCACGTGCTGTTCTACCCGCACAAGCCACTGGTCACCACTCGCTCCATGGAGTAC
			CTGCGCTTCAGGGAGCTTCCTGCCGGCATCAACTCTGTGGTCGCCATCGCCTGCTAC
			ACTGGATACAACCAGGAGGACAGTGTCATTCTCAACGCCTCCGCTGTCGAGCGCGG
			ATTCTTCAGATCGGTTTTCTTCCGATCTTACAAAGATGCAGAATCGAAGCGTATTGGC
			GACCAAGAGGAGCAATTCGAGAAGCCCACCAGACAGACGTGTCAGGGAATGAGGAA
			TGCCATTTATGACAAATTGGACGATGATGGCATCATTGCTCCCGGTCTGAGAGTGTCT
			GGTGACGATGTGGTTATTGGCAAAACCATAACACTGCCCGATAATGATGACGAGCTG
			GAAGGTACAACAAAGAGGTTCACGAAGAGAGATGCCAGTACTTTCCTGCGTAACAGT
			GAGACGGGAATCGTCGACCAAGTCATGTTAACCTTGAACTCTGAGGGTTACAAGTTC
			TGCAAAATTCGAGTCAGGTCTGTGCGTATCCCGCAGATTGGCGATAAGTTCGCTTCC
			CGACATGGCCAAAAAGGAACGTGTGGAATACAGTATCGTCAAGAGGACATGCCTTTT
			ACAAGCGAGGGAATCGCACCGGATATTATTATCAATCCTCACGCTATCCCATCTCGTA
			TGACAATTGGCCATTTAATTGAATGTCTCCAAGGAAAGGTGTCGTCGAACAAGGGCG
			AGATAGGTGACGCGACGCCGTTCAAC

NL007	SEQ ID NO: 1129	SEQ ID NO: 1130	SEQ ID NO: 1083
	CGGTGTCCATTC	CGATGCAAGTAGG	TTTCAGAGATTTCCTTCTGAAACCTGAAATTTTGAGAGCAATCCTTGACTGTGGTTTTG
	ACAGYTCCGG	TGTCKGARTCYTC	AACATCCATCTGAAGTACAACATGAATGCATTCCTCAAGCTGTACTTGGAATGGACAT
			ATTGTGTCAAGCGAAATCCGGTATGGGAAAAACTGCTGTATTTGTGTTGGCGACATTA
			CAGCAAATTGAACCAACTGACAACCAAGTCAGTGTATTGGTCATGTGTCATACCAGAG
			AGCTTGCATTCCAAATCAGCAAAGAGTATGAACGATTTTCGAAATGTATGCCAAATAT
			CAAGGTTGGAGTTTTCTTCGGCGGACTGCCGATTCAGAGGGATGAGGAGACGTTGAA
			ATTGAACTGTCCTCACATCGTGGTTGGAACACCCGGACGAATTTTGGCGTTGGTACG
			CAACAAGAAGCTGGACCTCAAGCATCTCAAGCACTTTGTCCTTGACGAATGTGACAAA
			ATGTTGGAACTGTTAGATATGCGAAGAGATGTGCAGGAAATATTCCGAAACACGCCG
			CACAGCAAACAAGTCATGATGTTCAGTGCAACTCTCAGCAAAGAAATTCGTCCAGTCT
			GCAAGAAATTCATGCAAGATCCGATGGAAGTGTACGTTGATGACGAGGCCAAGCTGA
			CGCTTCACGGCCTGCAGCAGCACTATGTCAAACTCAAAGAAAACGAAAAGAACAAAA
			AGTTATTTGAATTACTTGACATACTTGAATTCAACCAGGTTGTTATATTTGTGAAGTCA
			GTGCAGCGCTGCATGGCCCTATCGCAACTCCTAACAGAGCAGAACTTCCCTGCAGTG
			GCTATTCACCGTGGCATGACACAAGAAGAACGATTGAAGAAATATCAAGAGTTCAAAG
			AGTTCCTAAAGCGAATTTTGGTAGCAACGAATCTGTTTGGCAGAGGAATGGATATTGA
			GAGAGTCAACATTGTATTCAACTATGACATGCCT

NL008	SEQ ID NO: 1131	SEQ ID NO: 1132	SEQ ID NO: 1085
	GTGGTGGATCA	GCGCATTTGATCGT	GGAAGGATAGAAAACCAGAAACGAGTTGTTGGTGTTCTTTTGGGATGCTGGAGACCT
	CTTYAAYCGKATG	TBGTYTTCAC	GGAGGTGTATTAGATGTTTCAAACAGTTTTGCAGTTCCATTTGATGAGGACGACAAAG
			AAAAGAATGTTTGGTTCTTAGACCATGATTACTTGGAAAACATGTTCGGGATGTTCAA
			GAAAGTTAATGCTAGAGAAAAGGTTGTGGGTTGGTACCATACTGGACCCAAACTCCA
			CCAAAACGATGTTGCAATCAATGAGTTGATTCGTCGTTACTGTCCAAACTGTGTCTTA
			GTCATAATCGATGCCAAGCCTAAAGATTTGGGTCTACCTACAGAGGCATACAGAGTC
			GTTGAAGAAATCCATGATGATGGATCGCCAACATCAAAAACATTTGAACATGTGATGA
			GTGAGATTGGGGCAGAAGAGGCTGAGGAGATTGGCGTTGAACATCTGTTGAGAGAC
			ATCAAAGATACAACAGTCGGGTCACTGTCACAGCGCGTCACAAATCAGCTGATGGGC
			TTGAAGGGCTTGCATCTGCAATTACAGGATATGCGAGACTATTTGAATCAGGTTGTCG
			AAGGAAAGTTGCCAATGAACCATCAAATCGTTTACCAACTGCAAGACATCTTCAACCT
			TCTACCCGATATCGGCCACGGCAATTTTGTAGACTCGCTCTAC

NL009	SEQ ID NO: 1133	SEQ ID NO: 1134	SEQ ID NO: 1087
	GGGCCGTGGTC	CCGCCAAAGGACT	TGCGACTATGATCGACCGCCGGGACGCGGTCAGGTGTGCGACGTCGACGTCAAGAA
	AGAAYATYWAYA	SARRTADCCCTC	CTGGTTTCCCTGCACCTCTGAGAACAATTTCAACTACCATCAATCGAGCCCTTGTGTT
	AC		TTTCTCAAACTGAACAAGATAATTGGTTGGCAACCGGAGTACTACAATGAGACTGAAG
			GCTTTCCAGATAATATGCCAGGTGACCTCAAGCGACACATTGCCCAACAGAAGAGTA
			TCAACAAGCTGTTTATGCAAACAATCTGGATAACTTGCGAAGGAGAGGGTCCTCTAGA
			CAAGGAGAATGCAGGGGAGATCCAGTACATCCCTAGACAGGGATTTCCGGGCTACTT
			CTACCCTTACACTAATGCC

NL010	SEQ ID NO: 1135	SEQ ID NO: 1136	SEQ ID NO: 1089 (amino terminus)
	CGGCTGACGTG	TGCCGGAAGTTCTC	GTCCAGTCGACTGGAAGCCACCAGGCTTGTTGTTCCCGTTGGATGTCTGTATCAACC
	GAAYGTKTGGCC	RTAYTCKGGC	TTTGAAGGAGAGACCTGATCTACCGCCTGTACAGTACGATCCAGTTCTTTGTACTAGG
			AATACTTGTCGTGCAATTCTGAATCCATTGTGCCAAGTCGACTATCGAGCCAAGCTAT
			GGGTCTGCAACTTTTGTTTCCAGAGGAATCCTTTCCCCCCTCAATATGCAGCTATTTC
			GGAGCAGCATCAACCAGCAGAACTGATACCTTCATTTTCCACCATCGAATACATCATT
			ACCAGAGCGCAAACGATGCCGCCGATGTTCGTGCTGGTGGTGGACACATGTCTGGA
			CGACGAGGAGCTGGGAGCTTTGAAGGACTCACTGCAGATGTCGCTGTCGCTGCTGC
			CGCCCAATGCACTCATCGGTCTCATCACGTTCGGCAAAATGGTGCAGGTGCACGAGC
			TTGGCTGCGACGGCTGCTCGAAGAGCTACGTGTTCCGTGGCGTGAAGGACCTGACT
			GCCAAGCAGATCCAGGACATGTTGGGCATTGGCAAGATGGCCGCCGCTCCACAGCC
			CATGCAACAGCGCATTCCCGGCGCCGCTCCCTCCGCACCTGTCAACAGATTTCTTCA
			GCCTGTCGGAAAGTGCGATATGAGTTTAACTGATCTGCTTGGGGAATTGCAAAGAGA
			TCCATGGAATGTGGCTCAGGGCAAGAGACCTCTCCGATCTACTGGAGTTGCATTGTC
			CATTGCAGTTGGTCTGCTCGAGTGCACA
			SEQ ID NO: 1115 (carboxy terminus)
			CGTTGAACGTGAAAGGCTCGTGTGTGTCAGACACTGACATTGGCTTGGGCGGCACCT
			CTCAATGGAAAATGTGCGCCTTCACTCCACACACAACTTGTGCATTCTTCTTCGAAGT
			TGTCAACCAGCACGCAGCCCCAATCCCACAGGGAGGAAGAGGATGCATCCAATTCAT
			TACGCAATACCAACATTCCAGTGGCCAGAGAAGGATACGTGTCACCACCATCGCTCG
			AAACTGGGCAGATGCGAGCACCAACCTGGCACACATCAGTGCCGGCTTCGACCAGG
			AGGCAGGAGCCGTGCTGATGGCCCGCATGGTCGTGCATCGCGCCGAGACTGACGAT
			GGACCTGACGTCATGCGCTGGGCTGACCGCATGCTCATCCGTCTCTGTCAGAGGTTC
			GGTGAATACAGTAAGGATGACCCTAACAGTTTCCGTCTGCCAGAGAACTTCACACTTT
			ATCCGCAGTTCATGTACCATCTGCGTCGATCCCAATTCTTGCAAGTGTTCAACAACAG
			TCCTGATGAAACATCTTACTACAGGCACATTCTTATGCGAGAGGATCTGACTCAGAGT
			TTGATTATGATCCAGCCGATTTTGTACAGCTACAGCTTCAATGGTCCCCCCGAGCCAG
			TGCTGCTCGACACCAGCAGTATTCAACCCGACAGAATCCTATTGATGGACACATTTTT
			CCAAATTCTCATTTTCCATGGAGAGACGATTGCTCAATGGCGATCTCTGGGCTACCAG
			GACAT

NL011	SEQ ID NO: 1137	SEQ ID NO: 1138	SEQ ID NO: 1091
	CCCACTTTCAAG	CGCTCTCTCTCGAT	AGATGGTGGTACCGGCAAAACTACATTTGTCAAACGACATCTTACCGGAGAATTTGAA
	TGYGTRYTRGTC	CTGYDSCTGCC	AAGAAGTATGTTGCCACCCTTGGAGTTGAAGTTCACCCCCTTGTATTTCACACAAACA
	GG		GAGGTGTGATTAGGTTCAATGTGTGGGACACAGCTGGCCAGGAAAAGTTCGGTGGA
			CTTCGTGATGGATATTACATTCAGGGACAATGCGCCATCATTATGTTTGACGTAACGT
			CAAGAGTCACCTACAAGAACGTTCCCAACTGGCACAGAGATTTAGTGAGGGTTTGCG
			AAAACATTCCCATTGTACTATGCGGCAACAAAGTAGACATCAAGGACAGGAAAGTCAA
			GGCCAAGAGCATAGTCTTCCATAGGAAGAAGAACCTTCAGTACTACGACATCAGTGC
			GAAAAGCAACTACAACTTCGAGAAGCCGTTCCTGTGGTTGGCAAAGAAGCTGATCGG
			TGACCCCAACCTGGAGTTCGTCGCCATGCCCGCCCTCCTCCCACCCGAGGTCACAAT
			GGACCCCCAAT

NL012	SEQ ID NO: 1139	SEQ ID NO: 1140	SEQ ID NO: 1093
	GCAGGCGCAGG	GAATTTCCTCTTSA	GCAGCAGACGCAGGCACAGGTAGACGAGGTTGTCGATATAATGAAAACAAACGTTGA
	TBGABGARGT	GYTTBCCVGC	GAAAGTATTGGAGAGGGATCAAAAACTATCAGAATTGGATGATCGAGCAGATGCTCTA
			CAGCAAGGCGCTTCACAGTTTGAACAGCAAGCTGGCAAACTCAAGAGGAAATTC

NL013	SEQ ID NO: 1141	SEQ ID NO: 1142	SEQ ID NO: 1095
	CAGATGCGCCC	GCCCTTGACAGAYT	CGCAGAGCAAGTCTACATCTCTTCACTGGCCTTATTGAAAATGCTTAAGCACGGTCGC
	GTBGTDGAYAC	GDATVGGATC	GCCGGTGTTCCCATGGAAGTTATGGGCCTAATGCTGGGCGAATTTGTAGACGACTAC
			ACTGTGCGTGTCATTGATGTATTCGCTATGCCACAGAGTGGAACGGGAGTGAGTGTG
			GAGGCTGTAGACCCGGTGTTCCAAGCGAAGATGTTGGACATGCTAAAGCAGACAGG
			ACGGCCCGAGATGGTGGTGGGCTGGTACCACTCGCACCCGGGCTTCGGCTGCTGG
			CTGTCGGGTGTCGACATCAACACGCAGGAGAGCTTCGAGCAACTATCCAAGAGAGC
			CGTTGCCGTCGTCGTC

NL014	SEQ ID NO: 1143	SEQ ID NO: 1144	SEQ ID NO: 1097
	CGCAGATCAAR	GAACTTGCGGTTGA	TTTCATTGAGCAAGAAGCCAATGAGAAAGCCGAAGAGATCGATGCCAAGGCCGAGGA
	CAYATGATGGC	BGTTSCGDCC	AGAATTCAACATTGAAAAGGGAAGGCTCGTACAGCACCAGCGCCTTAAAATCATGGA
			GTACTATGACAGGAAAGAGAAGCAGGTTGAGCTCCAGAAAAAAATCCAATCGTCAAA
			CATGCTGAACCAAGCGCGTCTGAAGGCACTGAAGGTGCGCGAAGATCACGTGAGAA
			GTGTGCTCGAAGAATCCAGAAAACGTCTTGGAGAAGTAACCAGAAACCCAGCCAAGT
			ACAAGGAAGTCCTCCAGTATCTAATTGTCCAAGGACTCCTGCAGCTGCTAGAATCAAA
			CGTAGTACTGCGCGTGCGCGAGGCTGACGTGAGTCTGATCGAGGGCATTGTTGGCT
			CATGCGCAGAGCAGTACGCGAAGATGACCGGCAAAGAGGTGGTGGTGAAGCTGGAC
			GCTGACAACTTCCTGGCCGCCGAGACGTGTGGAGGCGTCGAGTTGTTCGCCCGCAA
			CGGCCGCATCAAGATCCCCAACACCCTCGAGTCCAGGCTCGACCTCATCTCCCAGCA
			ACTTGTGCCCGAGATTAGAGTCGCGCTCTTT

NL015	SEQ ID NO: 1145	SEQ ID NO: 1146	SEQ ID NO: 1099
	GCCGCAAGGAG	GTCCGTGGGAYTC	ATTGTGCTGTCTGACGAGACATGTCCGTTCGAAAAGATCCGCATGAATCGAGTGGTC
	ACBGTVTGC	RGCHGCAATC	AGGAAGAATCTGCGAGTGCGCTTGTCCGACATTGTCTCGATCCAGCCTTGCCCAGAC
			GTCAAGTATGGAAAGCGTATCCATGTGCTGCCCATTGATGATACCGTTGAGGGTCTTA
			CAGGAAATCTGTTCGAAGTGTATTTGAAGCCATACTTCCTGGAAGCATACAGGCCAAT
			TCACAAGGATGATGCATTCATTGTTCGCGGAGGTATGAGAGCGGTCGAATTCAAGGT
			GGTTGAAACAGATCCATCGCCCTACTGCATTGTCGCGCCAGACACCGTCATCCATTG
			TGAGGGAGACCCCATCAAACGTGAGGATGAAGAAGACGCAGCAAACGCAGTCGGCT
			ACGACGACATTGGAGGCTGCAGAAAGCAGCTGGCGCAGATCAAAGAGATGGTGGAG
			TTGCCGCTGAGACATCCCAGTCTGTTCAAGGCGATCGGCGTGAAGCCGCCACGAGG
			CATCCTGCTGTACGGACCACCGGGAACCGGAAAGACGTTGATAGCGCGCGCCGTCG
			CCAACGAAACGGGCGCCTTCTTCTTCCTCATCAACGGACCCGAGATTATGAGCAAAT
			TGGCCGGCGAGTCGGAGAGTAACCTGCGCAAAGCTTTCGAGGAAGCGGACAAAAAC
			GCACCGGCCATCATCTTCATCGATGAGCTGGACGCAATCGCGCCAAAACGCGAGAA
			GACGCACGGCGAGGTGGAGCGACGCATCGTGTCGCAGCTGCTGACGCTGATGGAC
			GGTCTCAAGCAGAGCTCGCACGTGATTGTCATGGCCGCCACCAATCGGCCCAACTC
			GATCGATGCCGCGCTTAGGCGCTTTGGCCGCTTTGATCGCGAAATCGACATTGGCAT
			TCCCGATGCCACCGGTCGTCTCGAGGTGCTGCGCATCCACACCAAGAACATGAAGTT
			GGCTGATGACGTCGATTTGGAACA

NL016	SEQ ID NO: 1147	SEQ ID NO: 1148	SEQ ID NO: 1101
	GTTCACCGGCG	CGGCATAGTCAGA	GACGCCAGTATCAGAAGACATGCTTGGTCGTGTATTCAACGGAAGTGGTAAGCCCAT
	AYATYCTGCG	ATSGGRATCTG	CGACAAAGGACCTCCCATTCTTGCTGAGGATTATCTCGACATTCAAGGTCAACCCATC
			AATCCTTGGTCGCGTATCTATCCCGAGGAAATGATCCAGACTGGAATTTCAGCCATCG
			ACGTCATGAACTCGATTGCTCGTGGCCAGAAAATCCCCATCTTTTCAGCTGCCGGTCT
			ACCTCACAACGAAATTGCTGCTCAAATCTGTAGACAGGCTGGTCTTGTCAAACTGCCA
			GGAAAGTCAGTTCTCGATGACTCTGAGGACAACTTTGCTATTGTATTCGCAGCCATGG
			GAGTCAACATGGAAACTGCTCGATTCTTCAAACAGGATTTCGAGGAGAACGGCTCTAT
			GGAGAACGTGTGCCTGTTCTTGAACCTGGCGAACGACCCGACGATCGAGCGTATCAT
			CACACCACGCCTGGCGCTGACGGCCGCCGAGTTCCTGGCCTACCAGTGCGAGAAGC
			ACGTGCTCGTCATCCTCACCGACATGAGCTCCTACGCCGAGGCGCTGCGAGAGGTG
			TCCGCCGCCCGCGAGGAGGTGCCCGGCCGTCGTGGTTTCCCCGGTTACATGTACAC
			CGATCTGGCCACCATCTACGAGCGCGCCGGACGAGTCGAGGGTCGCAACGGCTCCA
			TCACG

NL018	SEQ ID NO: 1149	SEQ ID NO: 1150	SEQ ID NO: 1103
	GCTCCGTCTACA	GTGCATCGGTACC	TATGCAAATGCCTGTGCCACGCCCACAAATAGAAAGCACACAACAGTTTATTCGATCC
	THCARCCNGAR	AHSCHGCRTC	GAGAAAACAACATACTCGAATGGATTCACCACCATTGAGGAGGACTTCAAAGTAGACA
	GG		CTTTCGAATACCGTCTTCTGCGCGAGGTGTCGTTCCGCGAATCTCTGATCAGAAACTA
			CTTGCACGAGGCGGACATGCAGATGTCGACGGTGGTGGACCGAGCATTGGGTCCCC
			CCTCGGCGCCACACATCCAGCAGAAGCCGCGCAACTCAAAAATCCAGGAGGGCGGC
			GATGCCGTCTTTTCCATCAAGCTCAGCGCCAACCCCAAGCCTCGGCTGGTCTGGTTC
			AAGAACGGTCAGCGCATCGGTCAGACGCAGAAACACCAGGCCTCCTACTCCAATCAG
			ACCGCCACGCTCAAGGTCAACAAAGTCAGCGCTCAAGACTCCGGCCACTACACGCT
			GCTTGCTGAAAATCCGCAAGGATGTACTGTGTCCTCAGCTTACCTAGCTGTCGAATCA
			GCTGGCACTCAAGATACAGGATACAGTGAGCAATACAGCAGACAAGAGGTGGAGAC
			GACAGAGGCGGTGGACAGCAGCAAGATGCTGGCACCGAACTTTGTTCGCGTGCCGG
			CCGATCGCGACGCGAGCGAAGGCAAGATGACGCGGTTTGACTGCCGCGTGACGGG
			CCGACCCTACCCGGACGTGGCCTGGTTCATCAACGGCCAACAGGTGGCTGACGACG
			CCACGCACAAGATCCTCGTCAACGAGTCTGGCAACCACTCGCTCATGATCACCGGCG
			TCACTCGCTTGGACCACGGAGTGGTCGGCTGTATTGCCCGCAACAAGGCTGGCGAA
			ACCTCATTCCAGTGCAACTTGAATGTGATCGAGAAAGAACTGGTTGTGGCGCCGAAA
			TTTGTGGAGAGATTCGCACAAGTGAATGTGAAGGAGGGTGAGCCGGTTGTGCTGAG
			CGCACGCGCTGTTGGCACACCTGTTCCAAGAATAACATGGCAGAAGGACGGCGCCC
			CGATCCAGTCGGGACCGAGCGTGAGTCTGTTTGTGGACGGAGGTGCGACCAGCCTG
			GACATCCCGTACGCGAAGGCGTCG

NL019	SEQ ID NO: 1151	SEQ ID NO: 1152	SEQ ID NO: 1105
	GTCCTGTCTGCT	CCTTGATCTCHGC	CGATGACACATACACAGAAAGTTACATCAGTACCATTGGTGTAGATTTTAAAATTAGAA
	GCTVMGWTTYGC	MGCCATBGTC	CAATAGATCTCGATGGAAAAACCATAAAGCTTCAGATTTGGGACACGGCCGGCCAGG
			AGCGGTTCCGCACGATCACATCGAGCTACTACCGGGGCGCCCACGGCATCATTGTG
			GTGTACGACTGCACCGACCAGGAGTCGTTCAACAACCTCAAACAGTGGCTCGAGGA
			GATTGACCGCTACGCCTGTGATAATGTCAACAAACTGCTCGTCGGCAACAAGTGTGA
			TCAGACCAACAAAAAGGTCGTCGACTATACACAGGCTAAGGAATACGCCGACCAGCT
			GGGCATTCCGTTCCTGGAGACGTCGGCGAAGAACGCGACCAATGTGGAGCAGGCGT
			TCAT

NL021	SEQ ID NO: 1153	SEQ ID NO: 1154	SEQ ID NO: 1107
	CTCAATCAGAGC	GGAATTGCCSAGV	CGTCAGTCTCAATTCTGTCACCGATATCAGCACCACGTTCATTCTCAAGCCACAAGAG
	GTYCCHCCRTAY	CGDGADCC	AACGTGAAGATAACGCTTGAGGGCGCACAGGCCTGTTTCATTTCACACGAACGACTT
	GG		GTGATCTCACTGAAGGGAGGAGAACTCTATGTTCTAACTCTCTATTCCGATAGTATGC
			GCAGTGTGAGGAGTTTTCATCTGGAGAAAGCTGCTGCCAGTGTCTTGACTACTTGTAT
			CTGTGTTTGTGAGGAGAACTATCTGTTCCTTGGTTCCCGTCTTGGAAACTCACTGTTG
			CTCAGGTTTACTGAGAAGGAATTGAACCTGATTGAGCCGAGGGCCATCGAAAGCTCA
			CAGTCCCAGAATCCGGCCAAGAAGAAAAAGCTGGATACTTTGGGAGATTGGATGGCA
			TCTGACGTCACTGAAATACGCGACCTGGATGAACTAGAAGTGTATGGCAGTGAAACA
			CAAACCTCTATGCAAATTGCATCCTACATATTC

NL022	SEQ ID NO: 1155	SEQ ID NO: 1156	SEQ ID NO: 1109
	GCGTGCTCAAG	CCAGTTCATGCTTR	TACATTGCACAGAGAATTCCTTTCCGAGCCAGATCTGCAATCTTACAGTGTTATGATA
	TAYATGACBGAY	TANGCCCANGC	ATTGATGAAGCTCACGAGAGGACGTTGCACACTGATATACTGTTCGGTTTGGTGAAA
	GG		GATGTCGCCCGATTCAGACCTGACTTGAAGCTGCTCATATCAAGCGCCACACTGGAT
			GCTCAGAAATTCTCCGAGTTTTTCGACGATGCACCCATCTTCAGGATTCCGGGCCGT
			AGATTTCCGGTGGACATCTACTACACAAAGGCGCCCGAGGCTGACTACGTGGACGCA
			TGTGTCGTTTCGATCCTGCAGATCCACGCCACTCAGCCGCTGGGAGACATCCTGGTC
			TTCCTCACCGGTCAGGAGGAGATCGAAACCTGCCAGGAGCTGCTGCAGGACAGAGT
			GCGCAGGCTTGGGCCTCGTATCAAGGAGCTGCTCATATTGCCCGTCTATTCCAACCT
			ACCCAGTGATATGCAGGCAAAGATTTTCCTGCCCACTCCACCAAATGCTAGAAAGGTA
			GTATTGGCCACAAATATTGCAGAAACCTCATTGACCATCGACAATATAATCTACGTGA
			TTGATCCTGGTTTTTGTAAGCAGAATAACTTCAATTCAAGGACTGGAATGGAATCGCT
			TGTTGTAGTGCCTGTTTCAAAGGCATCGGCCAATCAGCGAGCAGGGCGGGCGGGAC
			GGGTGGCGGCCGGCAAGTGCTTCCGTCTGTACACG

NL023	SEQ ID NO: 1157	SEQ ID NO: 1158	SEQ ID NO: 1111
	CCGGAGCTTCT	GAAAGCACACGCT	CCGGAGCTTCTCTCAGGAACGCCAGCACGAGGAAATGAAGGAATCCTCGGGTCGCA
	CTCAGGAACGC	GTTGCTCTGG	TGCATCACAGCGATCCTCTAATCGTCGAGACTCATAGCGGTCACGTGAGAGGAATCT
			CGAAGACCGTCCTCGGACGGGAGGTCCACGTGTTTACCGGGATTCCGTTTGCGAAA
			CCTCCCATCGGTCCGTTGCGATTCCGTAAACCGGTTCCCGTCGACCCGTGGCACGG
			CGTTCTGGATGCGACCGCGCTTCCCAACAGCTGCTACCAGGAACGGTACGAGTATTT
			CCCGGGCTTCGAGGGAGAGGAAATGTGGAATCCGAATACGAATTTGTCCGAAGATTG
			TCTGTATTTGAACATATGGGTGCCGCACCGGTTGAGAATCCGACACAGAGCCAACAG
			CGAGGAGAATAAACCAAGAGCGAAGGTGCCGGTGCTGATCTGGATCTACGGCGGGG
			GTTACATGAGCGGCACAGCTACACTGGACGTGTACGATGCTGACATGGTGGCCGCC
			ACGAGTGACGTCATCGTCGCCTCCATGCAGTACCGAGTGGGTGCGTTCGGCTTCCTC
			TACCTCGCACAGGACTTGCCTCGAGGCAGCGAGGAGGCGCCGGGCAACATGGGGC
			TCTGGGACCAGGCCCTTGCCATCCGCTGGCTCAAGGACAACATTGCCGCCTTTCGGA
			GGCGATCCCGAACTCATGACGCTCTTTGGCGAGTCGGCTGGGGGTGGATCTGTAAG
			CATCCACTTGGTATCACCGATAACTCGCGGCCTAGCGCGTCGTGGCATCATGCAGTC
			AGGAACGATGAACGCACCGTGGAGCTTCATGACGGCGGAACGCGCGACCGAAATCG
			CCAAGACGCTCATTGACGACTGCGGCTGCAACTCGTCGCTCCTGACCGACGCTCCC
			AGTCGCGTCATGTCCTGTATGCGATCAGTCGAGGCAAAGATCATCTCCGTGCAGCAA
			TGGAACAGCTACTCCGGCATTCTCGGACTTCCGTCTGCACCCACCATCGACGGCATT
			TTCCTGCCCAAACATCCCCTCGATCTGCTCAAGGAAGGCGACTTTCAGGACACTGAA
			ATACTCATCGGCAGTAATCAGGATGAGGGTACCTACTTCATATTGTACGATTTCATCG
			ACTTCTTCCAAAAAGACGGGCCGAGTTTCTTGCAAAGAGATAAGTTCCTAGACATCAT
			CAACACAATTTTCAAGAATATGACGAAAATTGAGAGGGAAGCTATCATATTCCAGTAC
			ACAGATTGGGAGCATGTTATGGATGGTTATCTGAACCAGAAAATGATCGGAGATGTG
			GTTGGTGATTACTTCTTCATCTGTCCGACAAATCATTTCGCACAGGCATTCGCAGAGC
			ATGGAAAGAAGGTGTATTACTATTTCTTCACCCAGAGAACCAGTACAAGTTTATGGGG
			CGAGTGGATGGGAGTCATGCATGGAGATGAAATAGAATACGTTTTTGGTCATCCTCTC
			AACATGTCGCTGCAATTCAATGCTAGGGAAAGGGATCTCAGTCTGCGAATAATGCAA
			GCTTACTCTAGGTTTGCATTGACAGGTAAACCAGTGCCTGATGACGTGAATTGGCCTA
			TCTACTCCAAGGACCAGCCGCAGTATTACATTTTCAATGCGGAGACTTCGGGCACAG
			GCAGAGGACCCAGAGCAACAGCGTGTGCTTTC

NL027	SEQ ID NO: 1159	SEQ ID NO: 1160	SEQ ID NO: 1113
	GCCGATCGTKYT	GGTATAGATGAARC	AGAAGACGGCACGGTGCGTATTTGGCACTCGGGCACCTACAGGCTGGAGTCCTCGC
	VACKGGCTC	ARTCDCCVACCCA	TGAATTATGGCCTCGAAAGAGTGTGGACCATTTGCTGCATGCGAGGATCCAACAATG
			TGGCTCTTGGCTACGACGAAGGCAGCATAATGGTGAAGGTGGGTCGGGAGGAGCCG
			GCCATCTCGATGGATGTGAACGGTGAGAAGATTGTGTGGGCGCGCCACTCGGAGAT
			ACAACAGGTCAACCTCAAGGCCATGCCGGAGGGCGTCGAAATCAAAGATGGCGAAC
			GACTGCCGGTCGCCGTTAAGGATATGGGCAGCTGTGAAATATATCCGCAGACCATCG
			CTCATAATCCCAACGGCAGATTCCTAGTCGTTTGTGGAGATGGAGAGTACATAATTCA
			CACATCAATGGTGCTAAGAAATAAGGCGTTTGGCTCGGCCCAAGAGTTCATTTGGGG
			ACAGGACTCGTCCGAGTATGCTATCAGAGAAGGAACATCCACTGTCAAAGTATTCAAA
			AACTTCAAAGAAAAGAAATCATTCAAGCCAGAATTTGGTGCTGAGAGCATATTCGGCG
			GCTACCTGCTGGGAGTTTGTTCGTTGTCTGGACTGGCGCTGTACGACTGGGAGACCC
			TGGAGCTGGTGCGTCGCATCGAGATCCAACCGAAACACGTGTACTGGTCGGAGAGT
			GGGGAGCTGGTGGCGCTGGCCACTGATGACTCCTACTTTGTGCTCCGCTACGACGC
			ACAGGCCGTGCTCGCTGCACGCGACGCCGGTGACGACGCTGTCACGCCGGACGGC
			GTCGAGGATGCATTCGAGGTCCTTGGTGAAGTGCACGAAACTGTAAAAACTGGATTG

TABLE 2-CS

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

CS001	SEQ ID NO: 1706	SEQ ID NO: 1707	SEQ ID NO: 1682
	CATTTGAAGCGT	CTTCGTGCCCTT	TAAAGCATGGATGTTGGACAAACTGGGTGGCGTGTACGCGCCGCGGCCGTCGACCGG
	TTWRMYGCYCC	GCCRATKATRAA	CCCCCACAAGTTGCGCGAGTGCCTGCCGCTGGTGATCTTCCTCAGGAACCGGCTCAA
		BACG	GTACGCGCTCACCGGAAATGAAGTGCTTAAGATTGTAAAGCAGCGACTTATCAAAGTTG
			ACGGCAAAGTCAGGACAGACCCCACATATCCCGCTGGATTTATGGATGTTGTTTCCATT
			GAAAAGACAAATGAGCTGTTCCGTCTTATATATGATGTCAAAGGCAGATTTACTATTCAC
			CGTATTACTCCTGAGGAGGCTAAATACAAGCTGTGCAAGGTGCGGCGCGTGGCGACG
			GGCCCCAAGAACGTGCCTTACCTGGTGACCCACGACGGACGCACCGTGCGATACCCC
			GACCCACTCATCAAGGTCAACGACTCCATCCAGCTCGACATCGCCACCTCCAAGATCA
			TGGACTTCATCAAGTTTGAATCTGGTAACCTATGTATGATCACGGGAGGCCGTAACTTG
			GGGCGCGTGGGCACCATCGTGTCCCGCGAGCGACATCCCGGGTCCTTCGACATCGTG
			CATATACGGGACTCCACCGGACATACCTTCGCTACCAGATTGAACAACGTGTTCATAAT
			CGGCAAGGGCACGAAG

CS002	SEQ ID NO: 1708	SEQ ID NO: 1709	SEQ ID NO: 1684
	GAGTTTCTTTAG	GCAATGTCATCC	GAGTTTCTTTAGTAAAGTATTCGGTGGCAAGAAGGAGGAGAAGGGTCCATCAACACAC
	TAAAGTATTCGG	ATCAKRTCRTGTAC	GAAGCTATACAGAAATTACGCGAAACGGAAGAGTTATTGCAGAAGAAACAAGAGTTTCT
	TGG		AGAGCGAAAGATCGACACTGAATTACAAACGGCGAGAAAACATGGCACAAAGAATAAG
			AGAGCTGCCATTGCGGCACTGAAGCGCAAGAAGCGTTATGAAAAGCAGCTTACCCAGA
			TTGATGGCACGCTTACCCAAATTGAGGCCCAAAGGGAAGCGCTAGAAGGAGCTAACAC
			CAATACACAGGTGCTTAACACTATGCGAGATGCTGCTACCGCTATGAGACTCGCCCAC
			AAGGATATCGATGTAGACAAGGTACACGATCTGATGGATGACATTGC

CS003	SEQ ID NO: 1710	SEQ ID NO: 1711	SEQ ID NO: 1686
	CAGGAGTTGAR	CAGGTTCTTCCT	TGGTCTCCGCAACAAGCGTGAGGTGTGGAGGGTGAAGTACACGCTGGCCAGGATCCG
	RATHATYGGHSA	CTTKACRCGDCC	TAAGGCTGCCCGTGAGCTGCTCACACTCGAGGAGAAAGACCCTAAGAGGTTATTCGAA
	RTA		GGTAATGCTCTCCTTCGTCGTCTGGTGAGGATCGGTGTGTTGGATGAGAAGCAGATGA
			AGCTCGATTATGTACTCGGTCTGAAGATTGAGGACTTCTTGGAACGTCGTCTCCAGACT
			CAGGTGTTCAAGGCTGGTCTAGCTAAGTCTATCCATCATGCCCGTATTCTTATCAGACA
			GAGGCACATCCGTGTCCGCAAGCAAGTTGTGAACATCCCTTCGTTCATCGTGCGGCTG
			GACTCTGGCAAGCACATTGACTTCTCGCTGAAGTCTCCGTTCGGCGGCGGCCGGCCG

CS006	SEQ ID NO: 1712	SEQ ID NO: 1713	SEQ ID NO: 1688
	ACCTGCCAAGG	GAGATCTTCTGC	ACCTGCCAAGGAATGAGGAACGCTTTGTATGACAAATTGGATGATGATGGTATAATTGC
	AATGMGVAAYGC	ACRTTKACVGCATC	ACCAGGGATTCGTGTATCTGGTGACGATGTAGTCATTGGAAAAACTATAACTTTGCCAG
			AAAACGATGATGAGCTGGAAGGAACATCAAGACGATACAGTAAGAGAGATGCCTCTAC
			ATTCTTGCGAAACAGTGAAACTGGTATTGTTGACCAAGTTATGCTTACACTTAACAGCG
			AAGGATACAAATTTTGTAAAATACGTGTGAGATCTGTGAGAATCCCACAAATTGGAGAC
			AAATTTGCTTCTCGTCATGGTCAAAAAGGGACTTGTGGTATTCAATATAGGCAAGAAGA
			TATGCCTTTCACTTGTGAAGGATTGACACCAGATATTATCATCAATCCACATGCTATCCC
			CTCTCGTATGACAATTGGTCACTTGATTGAATGTATTCAAGGTAAGGTCTCCTCAAATAA
			AGGTGAAATAGGTGATGCTACACCATTTAACGATGCTGTCAACGTGCAGAAGATCTC

CS007	SEQ ID NO: 1714	SEQ ID NO: 1715	SEQ ID NO: 1690
	CGGTGTCCATTC	CGATGCAAGTAG	TTTCAGAGATTTCTTGTTGGAACCAGAGATTTTGGGGGCTATCGTCGATTGCGGTTTCG
	ACAGYTCCGG	GTGTCKGARTCY	AGCACCCTTCAGAAGTTCAACATGAATGTATTCCCCAAGCTGTTTTGGGAATGGATATT
		TC	CTTTGTCAAAGCTAAATCCGGAATGGGAAAAACCGCCGTATTTGTTTTAGCAACACTGC
			AACAGCTAGAACCTTCAGAAAACCATGTTTACGTATTAGTAATGTGCCATACAAGGGAA
			CTCGCTTTCCAAATAAGCAAGGAATATGAGAGGTTCTCTAAATATATGGCTGGTGTTAG
			AGTATCTGTATTCTTTGGTGGGATGCCAATTCAGAAAGATGAAGAAGTATTGAAGACAG
			TGGCACAGGAGTGTCGGTTGAAGCTGTAGATCCTGTCTTCCAAGCAAAGATGTTGGAT
			ATGTTGAAGCAAACTGGACGACCTGAGATGGTAGTGGGATGGTACCACTCGCATCCTG
			GCTTTGGATGTTGGTTATCTGGAGTCGACATTAATACTCAGCAGTCTTTCGAAGCTTTG
			TCTGAACGTGCTGTAGCTGTAGTGGTTGATCCCATTCAGTCTGTCAAGGGC

CS014	SEQ ID NO: 1722	SEQ ID NO: 1723	SEQ ID NO: 1698
	ATGGCACTGAG	GAACTTGCGGTT	TTCAAAAGCAGATCAAGCATATGATGGCCTTCATCGAACAAGAGGCTAATGAAAAGGCC
	CGAYGCHGATG	GABGTTSCGDCC	GAGGAAATCGATGCAAAGGCCGAAGAGGAGTTCAACATTGAAAAAGGCCGCCTGGTG
			CAGCAGCAGCGGCTCAAGATCATGGAATACTACGAAAAGAAAGAGAAACAAGTGGAAC
			TCCAGAAAAAGATCCAATCTTCGAACATGCTGAATCAAGCCCGTCTGAAGGTGCTCAAA
			GTGCGTGAGGACCACGTACGCAACGTTCTCGACGAGGCTCGCAAGCGCCTGGCTGAG
			GTGCCCAAAGACGTGAAACTTTACACAGATCTGCTGGTCACGCTCGTCGTACAAGCCC
			TATTCCAGCTCATGGAACCCACAGTAACAGTTCGCGTTAGGCAGGCGGACGTCTCCTT
			AGTACAGTCCATATTGGGCAAGGCACAGCAGGATTACAAAGCAAAGATCAAGAAGGAC
			GTTCAATTGAAGATCGACACCGAGAATTCCCTGCCCGCCGATACTTGTGGCGGAGTGG
			AACTTATTGCTGCTAGAGGGCGTATTAAGATCAGCAACACTCTGGAGTCTCGTCTGGA
			GCTGATAGCCCAACAACTGTTGCCCGAAATACGTACCGCATTGTTC

CS015	SEQ ID NO: 1724	SEQ ID NO: 1725	SEQ ID NO: 1700
	GCCGCAAGGAG	CGATCAAAGCGW	ATCGTGCTTTCAGACGATAACTGCCCCGATGAGAAGATCCGCATGAACCGCGTCGTGC
	ACBGTVTGC	CCRAAVCGACG	GAAACAACTTGCGTGTACGCCTGTCAGACATAGTCTCCATAGCGCCTTGTCCATCGGT
			CAAATATGGGAAACGGGTACATATATTGCCCATTGATGATTCTGTCGAGGGTTTGACTG
			GAAATTTATTCGAAGTCTACTTGAAACCATACTTCATGGAAGCTTATCGGCCTATCCATC
			GCGATGACACATTCATGGTTCGCGGGGGCATGAGGGCTGTTGAATTCAAAGTGGTGGA
			GACTGATCCGTCGCCGTATTGCATCGTCGCTCCCGACACAGTGATACACTGCGAAGGA
			GACCCTATCAAACGAGAGGAAGAAGAAGAAGCCCTAAACGCCGTAGGGTACGACGAC
			ATCGGTGGCTGTCGTAAACAGCTCGCTCAGATCAAAGAGATGGTCGAGTTGCCTCTAA
			GGCATCCGTCGCTGTTCAAGGCAATTGGTGTGAAGCCGCCACGTGGAATCCTCATGTA
			TGGGCCGCCTGGTACCGGCAAAACTCTCATTGCTCGGGCAGTGGCTAATGAAACTGGT
			GCATTCTTCTTTCTGATCAACGGGCCGGAGATCATGTCCAAACTCGCGGGCGAGTCCG
			AATCGAACCTTCGCAAGGCATTCGAGGAAGCGGACAAGAACTCCCCGGCTATAATCTT
			CATCGATGAACTGGATGCCATCGCACCAAAGAGGGAGAAGACTCACGGTGAAGTGGA
			GCGTCGTATTGTGTCGCAACTACTTACTCTTATGGATGGAATGAAGAAGTCATCGCACG
			TGATCGTAATGGCCGCCACCAACCGTCCGAATTCGATCGACCCGGCGCTA

CS016	SEQ ID NO: 1726	SEQ ID NO: 1727	SEQ ID NO: 1702
	GTTCACCGGCG	GTCGCGCAGGTA	AGGATGGAAGCGGGGATACGTTTGAGCATCTCCTTGGGGAAGATACGGAGCAGCTGC
	AYATYCTGCG	GAAYTCKGC	CAGCCGATGTCCAGCGACTCGAATACTGTGCGGTTCTCGTAGTTGCCCTGTGTGATGA
			AGTTCTTCTCGAACTTGGTGAGGAACTCGAGGTAGAGCAGATCGTCGGGTGTCAGGGC
			TTCCTCACCGACGACAGCCTTCATGGCCTGCACGTCCTTACCGATGGCGTAGCAGGCG
			TACAGCTGGTTGGAAACATCAGAGTGGTCCTTGCGGGTCATTCCCTCACCGATGGCAG
			ACTTCATGAGACGAGACAGGGAAGGCAGCACGTTTACAGGCGGGTAGATCTGTCTGTT
			GTGGAGCTGACGGTCTACGTAGATCTGTCCCTCAGTGATGTAGCCCGTTAAATCGGGA
			ATAGGATGGGTGATGTCGTCGTTGGGCATAGTCAAGATGGGGATCTGCGTGATGGATC
			CGTTTCTACCCTCTACACGCCCGGCTCTCTCGTAGATGGTGGCCAAATCGGTGTACAT
			GTAACCTGGGAAACCACGTCGTCCGGGCACCTCCTCACGGGCGGCGGACACTTCACG
			CAGAGCCTCCGCGTACGAAGACATGTCAGTCAAGATTACCAGCACGTGTTTCTCACAC
			TGGTAGGCCAAGAACTCAGCAGCAGTCAAGGCCAAACGTGGTGTGATGATTCTCTCAA
			TAGTGGGATCGTTGGCCAGATTCAAGAACAGGCACACGTTCTCCATGGAGCCGTTCTC
			CTCGAAGTCCTGCTTGAAGAACCGGGCCGTCTCCATGTTCACACCCATGGCGGCGAAC
			ACGATGGCAAAGTTGTCCTCGTGGTCGTCCAGCACAGATTTGCCGGGGATCTTTACAA
			GACCGGCTTGCCTACAGATCTGGGCGGCAATTTCGTTGTGTGGCAGACCGGCAGCCG
			AGAAAATGGGGATCTTTTGCCCGCGAGCAATGGAGTTCATCACGTCGATAGCGGAGAT
			ACCAGTCTGGATCATTTCCTCAGGGTAGATACGGGACCAGGGGTTGATGGGCTGTCCC
			TGGATGTCCAAAAAGTCTTCAGCAAGGATTGGGGGACCTTTGTCAATGGGTTTTCCAGA
			GCCGTTGAATACGCGACCCAACATGTCTTCGGAGACAGGGGTGC

CS018	SEQ ID NO: 1728	SEQ ID NO: 1729	SEQ ID NO: 1704
	GCTCCGTCTACA	GTGCATCGGTAC	GCTCCGTCTACATTCAGCCGGAAGGCGTCCCTGTACCTGCTCAGCAATCCCAACAGCA
	THCARCCNGAR	CAHSCHGCRTC	GCAGAGTTACCGCCACGTCAGCGAGAGCGTCGAACACAAATCCTACGGCACGCAAGG
	GG		GTACACCACTTCGGAACAGACCAAGCAGACACAGAAGGTGGCGTACACCAACGGTTCC
			GACTACTCTTCCACGGACGACTTTAAGGTGGATACGTTCGAATACAGACTCCTCCGAG
			AAGTTTCGTTCAGGGAATCCATCACGAAGCGGTACATTGGCGAGACAGACATTCAGAT
			CAGCACGGAGGTCGACAAGTCTCTCGGTGTGGTGACCCCTCCTAAGATAGCACAAAAG
			CCTAGGAATTCCAAGCTGCAGGAGGGAGCCGACGCTCAGTTTCAAGTGCAGCTGTCG
			GGTAACCCGCGGCCACGGGTGTCATGGTTCAAGAACGGGCAGAGGATAGTCAACTCG
			AACAAACACGAAATCGTCACGACACATAATCAAACAATACTTAGGGTAAGAAACACACA
			AAAGTCTGATACTGGCAACTACACGTTGTTGGCTGAAAATCCTAACGGATGCGTCGTCA
			CATCGGCATACCTGGCCGTGGAGTCGCCTCAAGAAACTTACGGCCAAGATCATAAATC
			ACAATACATAATGGACAATCAGCAAACAGCTGTAGAAGAAAGAGTAGAAGTTAATGAAA
			AAGCTCTCGCTCCGCAATTCGTAAGAGTCTGCCAAGACCGCGATGTAACGGAGGGGAA
			AATGACGCGATTCGATTGCCGCGTCACGGGCAGACCTTACCCAGAAGTCACGTGGTTC
			ATTAACGATAGACAAATTCGAGACGATTATWATCATAAGATATTAGTAAACGAATCGTGT
			AATCATGCACTTATGATTACAAACGTCGATCTCAGTGATAGTGGCGTAGTATCATGTATA
			GCACGCAACAAGACCGGCGAAACTTCGTTTCAGTGTAGGCTGAACGTGATAGAGAAGG
			AGCAAGTGGTCGCTCCCAAATTCGTGGAGCGGTTCAGCACGCTCAACGTGCGCGAGG
			GCGAGCCCGTGCAGCTGCACGCGCGCGCCGTCGGCACGCCTACGCCACGCATCACA
			TGGCAGAAGGACGGCGTTCAAGTTATACCCAATCCAGAGCTACGAATAAATACCGAAG
			GTGGGGCCTCGACGCTGGACATCCCTCGAGCCAAGGCGTCGGACGCGGGATGGTAC
			CGATGCAC

TABLE 2-PX

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

PX001	SEQ ID NO: 2110	SEQ ID NO: 2111	SEQ ID NO: 2100
	GGCCCCAAGAAG	CTTCGTGCCCTTGC	GGCCCCAAGAAGCATTTGAAGCGCCTGAACGCGCCGCGCGCATGGATGCTGGA
	CATTTGAAGCG	CRATKATRAABACG	CAAGCTCGGCGGCGTGTACGCGCCGCGGCCCAGCACGGGCCCGCACAAGCTG
			CGCGAGTGCCTGCCGCTCGTCATCTTCCTGCAACCGCCTCAAGTACGCGCTCAG
			CGGCAACGAGGTGCTGAAGATCGTGAAGCAGCGCCTCATCAAGGTGGACGGCA
			AGGTCCGCACCGACCCCACCTACCCGGCTGGATTCATGGATGTTGTGTCGATTG
			AAAAGACCAATGAGCTGTTCCGTCTGATCTACGATGTGAAGGGACGCTTCACCAT
			CCACCGCATCACTCCCGAGGAGGCCAAGTACAAGCTGTGCAAGGTGAAGCGCG
			TGGCGACGGGCCCCAAGAACGTGCCGTACATCGTGACGCACAACGGCCGCACG
			CTGCGCTACCCCGACCCGCTCATCAAGGTCAACGACTCCATCCAGCTCGACATC
			GCCACCTGCAAGATCATGGACATCATCAAGTTCGACTCAGGTAACCTGTGCATGA
			TCACGGGAGGGCGTAACTTGGGGCGAGTGGGCACCATCGTGTCCCGCGAGAGG
			CACCCCGGGAGCTTCGACATCGTCCACATCAAGGACACCACCGGACACACCTTC
			GCCACCAGGTTGAACAACGTGTTCATCATCGGCAAGGGCACGAAG

PX009	SEQ ID NO: 2112	SEQ ID NO: 2113	SEQ ID NO: 2102
	GCACGTTGATCTG	GCAGCCCACGCYYT	GCACGTTGATCTGGTACAAAGGAACCGGTTACGACAGCTACAAGTATTGGGAGA
	GTACARRGGMACC	GCACTC	ACCAGCTCATTGACTTTTTGTCAGTATACAAGAAGAAGGGTCAGACAGCGGGTGC
			TGGTCAGAACATCTTCAACTGTGACTTCCGCAACCCGCCCCCACACGGCAAGGT
			GTGCGACGTGGACATCCGCGGCTGGGAGCCCTGCATTGATGAGAACCACTTCTC
			TTTCCACAAGTCTTCGCCTTGCATCTTCTTGAAGCTGAATAAGATCTACGGCTGG
			CGTCCAGAGTTCTACAACGACACGGCTAACCTGCCTGAAGCCATGCCCGTGGAC
			TTGCAGACCCACATTCGTAACATTACTGCCTTCAACAGAGACTATGCGAACATGG
			TGTGGGTGTCGTGCCACGGCGAGACGCCGGCGGACAAGGAGAACATCGGGCC
			GGTGCGCTACCTGCCCTACCCGGGCTTCCCCGGGTACTTCTACCCGTACGAGAA
			CGCCGAGGGGTATCTGAGCCCGCTGGTCGCCGTGCATTTGGAGAGGCCGAGGA
			CCGGCATAGTGATCAACATCGAGTGCAAAGCGTGGGCTGC

PX010	SEQ ID NO: 2114	SEQ ID NO: 2115	SEQ ID NO: 2104
	GTGGCTGCATACA	CGCGGCTGCTCCAT	GTGGCTGCATACAGTTCATTACGCAGTACCAGCACTCTAGTGGACAACGTCGCG
	GTTCATTACGCAG	GAAYASYTG	TTCGGGTCACCACTGTCGCGCGCAATTGGGGCGACGCAGCCGCCAACTTACAC
			CACATATCGGCGGGCTTCGACCAGGAGGCGGCGGCGGTGGTGATGGCGCGGC
			TGGTGGTGTACCGCGCGGAGCAGGAGGACGGGCCCGACGTGCTGCGCTGGCT
			CGACCGCATGCTCATACGCCTGTGCCAGAAGTTCGGCGAGTACGCGAAGGACG
			ACCCGAACAGCTTCCGTCTGTCGGAGAACTTCAGCCTGTACCCGCAGTTCATGT
			ACCACCTGCGCCGCTCGCAGTTCCTGCAGGTCTTCAACAACTCGCCCGACGAGA
			CCACCTTCTACAGACACATGCTGATGCGCGAAGACCTGACCCAATCCCTCATCAT
			GATCCAGCCGATCCTCTACTCGTACAGCTTCGGAGGCGCGCCCGAACCCGTGCT
			GTTAGACACCAGCTCCATCCAGCCCGACCGCATCCTGCTCATGGACACCTTCTT
			CCAGATCCTCATCTACCATGGAGAGACAATGGCGCAATGGCGCGCTCTCCGCTA
			CCAAGACATGGCTGAGTACGAGAACTTCAAGCAGCTGCTGCGAGCGCCCGTGG
			ACGACGCGCAGGAGATCCTGCAGACCAGGTTCCCCGTGCCGCGGTACATTGATA
			CAGAGCACGGCGGCTCACAGGCCCGGTTCTTGCTTTCCAAAGTGAATCCCTCTC
			AGACTCACAACAACATGTACGCGTATGGCGGGGCGATGCCGATACCATCAGCGG
			ACGGTGGCGCCCCCGTGTTGACGGATGACGTGTCGCTGCAAGTGTTCATGGAG
			CAGCCGCG

PX015	SEQ ID NO: 2116	SEQ ID NO: 2117	SEQ ID NO: 2106
	GCCGCAAGGAGA	GCAATGGCATCAAK	GCCGCAAGGAGACCGTGTGCATTGTGCTGTCCGACGACAACTGCCCCGACGAG
	CBGTVTGC	YTCRTCRATG	AAGATCCGCATGAACCGCGTCGTCCGGAACAACCTGCGAGTGCGCCTGTCAGAC
			ATTGTGTCCATCGCTCCTTGCCCGTCAGTGAAGTACGGCAAGAGAGTTCATATTC
			TGCCCATTGATGACTCTGTTGAGGGTTTGACTGGAAACCTGTTCGAAGTCTACCT
			GAAGCCGTACTTCATGGAGGCGTACCGGCCCATCCACCGCGACGACACGTTCAT
			GGTGCGCGGCGGCATGCGCGCCGTCGAGTTCAAGGTGGTGGAGACCGACCCCT
			CGCCCTACTGCATCGTGGCCCCCGACACGGTCATTCATTGTGAGGGAGAGCCGA
			TTAAACGCGAGGAAGAAGAGGAGGCTCTCAACGCCGTCGGCTACGACGACATC
			GGCGGGTGCCGCAAGCAGCTGGCGCAGATCAAGGAGATGGTGGAGCTGCCGCT
			GCGCCACCCCTCGCTGTTCAAGGCCATCGGGGTCAAGCCGCCGCGGGGGATAC
			TGATGTACGGGCCCCCGGGGACGGGGAAGACCTTGATCGCTAGGGCTGTCGCT
			AATGAGACGGGCGCATTCTTCTTCCTCATCAACGGCCCCGAGATCATGTCGAAA
			CTCGCCGGTGAATCCGAGTCGAACCTGCGCAAGGCGTTCGAGGAGGCGGACAA
			GAACTCTCCGGCCATCATCCTCATTGATGAACTTGATGCCATTGC

PX016	SEQ ID NO: 2118	SEQ ID NO: 2119	SEQ ID NO: 2108
	GTTCACCGGCGAY	CATCTCCTTGGGGA	GTTCACCGGCGATATTCTGCGCACGCCCGTCTCTGAGGACATGCTGGGTCGTAT
	ATYCTGCG	AGATACGCAGC	TTTCAACGGCTCCGGCAAGCCCATCGACAAGGGGCCCCCGATCCTGGCCGAGG
			AGTACCTGGACATCCAGGGGCAGCCCATCAACCCGTGGTCCCGTATCTACCCGG
			AGGAGATGATCCAGACTGGTATCTCCGCTATCGACGTGATGAACTCCATCGCCC
			GTGGTCAGAAGATCCCCATCTTCTCCGCCGCCGGTCTGCCCCACAACGAGATTG
			CTGCTCAGATCTGTAGGCAGGCTGGTCTTGTCAAGGTCCCCGGAAAATCCGTGT
			TGGACGACCACGAAGACAACTTCGCCATCGTGTTCGCCGCCATGGGAGTCAACA
			TGGAGACCGCCAGGTTCTTCAAGCAGGACTTCGAGGAGAACGGTTCCATGGAGA
			ACGTCTGTCTGTTCTTGAACTTGGCCAATGACCCGACCATTGAGAGGATTATCAC
			GCCGAGGTTGGCGCTGACTGCTGCCGAGTTCTTGGCCTACCAGTGCGAGAAACA
			CGTGTTGGTAATCTTGACCGACATGTCTTCATACGCGGAGGCTCTTCGTGAAGTG
			TCAGCCGCCCGTGAGGAGGTGCCCGGACGACGTGGTTTCCCAGGTTACATGTA
			CACGGATTTGGCCACAATCTACGAGCGCGCCGGGCGAGTCGAGGGCCGCAACG
			GCTCCATCACGCAGATCCCCATCCTGACCATGCCCAACGACGACATCACCCACC
			CCATCCCCGACTTGACCGGGTACATCACTGAGGGACAGATCTACGTGGACCGTC
			AGCTGCACAACAGGCAGATCTACCCGCCGGTGAATGTGCTCCCGTCGCTATCTC
			GTCTCATGAAGTCCGCCATCGGAGAGGGCATGACCAGGAAGGACCACTCCGAC
			GTGTCCAACCAACTGTACGCGTGCTACGCCATCGGCAAGGACGTGCAGGCGAT
			GAAGGCGGTGGTGGGCGAGGAGGCGCTCACGCCCGACGACCTGCTCTACCTCG
			AGTTCCTCACCAAGTTCGAGAAGAACTTCATCACACAGGGAAGCTACGAGAACC
			GCACAGTGTTCGAGTCGCTGGACATCGGCTGGCAGCCCCTGCGTATCTTCCCCA
			AGGAGATG

TABLE 2-AD

Target	Primer Forward	Primer Reverse	cDNA Sequence (sense strand)
ID	5′→3′	5′→3′	5′→3′

AD001	SEQ ID NO: 2374	SEQ ID NO: 2375	SEQ ID NO: 2364
	GGCCCCAAGAAGCA	CGCTTGTCCCG	GGCCCCAAGAAGCATTTGAAGCGTTTAAATGCTCCTAAAGCATGGATGTTGGACAA
	TTTGAAGCG	CTCCTCNGCRAT	ACTCGGAGGAGTATTCGCTCCTCGCCCCAGTACTGGCCCCCACAAATTGCGTGAA
			TGTTTACCTTTGGTGATTTTTCTTCGCAATCGGCTCAAGTATGCTCTGACGAACTGT
			GAAGTAACGAAGATTGTTATGCAGCGACTTATCAAAGTTGACGGCAAGGTGCGAAC
			CGATCCGAATTATCCCGCTGGTTTCATGGATGTTGTCACCATTGAGAAGACTGGAG
			AGTTCTTCAGGCTGGTGTATGATGTGAAAGGCCGTTTCACAATTCACAGAATTAGT
			GCAGAAGAAGCCAAGTACAAGCTCTGCAAGGTCAGGAGAGTTCAAACTGGGCCAA
			AAGGTATTCCATTCTTGGTGACCCATGATGGCCGTACTATCCGTTATCCTGACCCA
			GTCATTAAAGTTAATGACTCAATCCAATTGGATATTGCCACTTGTAAAATCATGGAC
			CACATCAGATTTGAATCTGGCAACCTGTGTATGATTACTGGTGGACGTAACTTGGG
			TCGAGTGGGGACTGTTGTGAGTCGAGAACGTCACCCAGGCTCGTTTGATATTGTT
			CATATCAAGGATACCCAAGGACATACTTTTGCCACAAGATTGAATAATGTATTCATC
			ATTGGAAAAGCTACAAAGCCTTACATTTCATTGCCAAAGGGTAAGGGTGTGAAATT
			GAGTATCGCCGAGGAGCGGGACAAGCG

AD002	SEQ ID NO: 2376	SEQ ID NO: 2377	SEQ ID NO: 2366
	GAGTTTCTTTAGTAA	GCAATGTCATCC	GAGTTTCTTTAGTAAAGTATTCGGTGGGAAGAAAGATGGAAAGGCTCCGACCACTG
	AGTATTCGGTGG	ATCAKRTCRTGT	GTGAGGCCATTCAGAAACTCAGAGAAACAGAAGAAATGTTAATCAAAAAGCAGGAA
		AC	TTTTTAGAGAAGAAAATCGAACAAGAAATCAATGTTGCAAAGAAAAATGGAACGAAA
			AATAAGCGAGCTGCTATTCAGGCTCTGAAAAGGAAAAAGAGGTATGAAAAACAATT
			GCAGCAAATTGATGGCACCTTATCCACAATTGAAATGCAAAGAGAAGCTTTGGAGG
			GTGCTAATACTAATACAGCTGTATTACAAACAATGAAATCAGCAGCAGATGCCCTTA
			AAGCAGCTCATCAGCACATGGATGTGGACAAGGTACATGACCTGATGGATGACATT
			GC

AD009	SEQ ID NO: 2378	SEQ ID NO: 2379	SEQ ID NO: 2368
	GAGTCCTAGCCGCV	CTGGATTCTCTC	GAGTCCTAGCCGCCTTGGTTGCAGTATGTTTATGGGTCTTCTTCCAGACACTGGAT
	YTSGTKGC	CCTCGCAMGAH	CCTCGTATTCCCACCTGGCAGTTAGATTCTTCTATCATTGGCACATCACCTGGCCT
		ACC	AGGTTTCCGGCCAATGCCAGAAGATAGCAATGTAGAGTCAACTCTCATCTGGTACC
			GTGGAACAGATCGTGATGACTTCCGTCAGTGGACAGACACCCTTGATGAATTTCTT
			GCTGTGTACAAGACTCCTGGTCTGACCCCTGGTCGAGGTCAGAACATCCACAACT
			GTGACTATGATAAGCCGCCAAAGAAAGGCCAAGTTTGCAATGTGGACATCAAGAAT
			TGGCATCCCTGCATTCAAGAGAATCACTACAACTACCACAAGAGCTCTCCATGCAT
			ATTCATCAAGCTCAACAAGATCTACAATTGGATCCCTGAATACTACAATGAGAGTAC
			GAATTTGCCTGAGCAGATGCCAGAAGACCTGAAGCAGTACATCCACAACCTGGAG
			AGTAACAACTCGAGGGAGATGAACACGGTGTGGGTGTCGTGCGAGGGAGAGAAT
			CCAG

AD015	SEQ ID NO: 2380	SEQ ID NO: 2381	SEQ ID NO: 2370
	GGATGAACTACAGC	GTCCGTGGGAY	GGATGAACTACAGCTTTTCCGAGGAGATACAGTTCTTCTTAAAGGAAAAAGGAGGA
	TBTTCCGHGG	TCRGCHGCAATC	AAGAAACTGTATGCATAGTGTTATCAGATGATACATGTCCTGATGGAAAAATAAGAA
			TGAATAGAGTTGTACGCAACAATTTACGTGTTCGTTTGTCAGATGTTGTATCTGTAC
			AACCTTGTCCTGATGTTAAGTATGGAAAAAGGATACATGTACTACCAATTGATGATA
			CAGTTGAAGGACTAACCGGGAATTTGTTTGAGGTGTACTTAAAACCGTACTTTCTC
			GAAGCATACCGACCCATTCACAAAGATGATGCGTTTATTGTTCGTGGTGGTATGCG
			AGCAGTAGAATTCAAAGTAGTGGAAACAGATCCTTCACCATATTGTATTGTTGCTCC
			TGATACTGTTATTCACTGTGAAGGTGATCCAATAAAACGTGAAGAGGAAGAAGAAG
			CATTAAATGCTGTTGGTTATGATGACATTGGGGGTTGCCGAAAACAGCTAGCACAG
			ATCAAGGAAATGGTGGAATTGCCATTACGGCACCCCAGTCTCTTTAAGGCTATTGG
			TGTTAAGCCACCGAGGGGAATACTGCTGTATGGACCCCCTGGAACTGGTAAAACC
			CTCATTGCCAGGGCTGTGGCTAATGAAACTGGTGCATTCTTCTTTTTAATAAATGGT
			CCTGAAATTATGAGCAAGCTTGCTGGTGAATCTGAAAGCAACTTACGTAAGGCATT
			TGAAGAAGCTGATAAGAATGCTCCGGCAATTATATTTATTGATGAACTAGATGCAAT
			TGCCCCTAAAAGAGAAAAAACTCATGGAGAGGTGGAACGTCGCATAGTTTCACAAC
			TACTAACTTTAATGGATGGTCTGAAGCAAAGTTCACATGTTATTGTTATGGCTGCCA
			CAAATAGACCCAACTCTATTGATGGTGCCTTGCGCCGCTTTGGCAGATTTGATAGG
			GAAATTGATATTGGTATACCAGATGCCACTGGTCGCCTTGAAATTCTTCGTATCCAT
			ACTAAGAATATGAAGTTAGCTGATGATGTTGATTTGGAACAGATTGCAGCCGAATC
			CCACGGAC

AD016	SEQ ID NO: 2382	SEQ ID NO: 2383	SEQ ID NO: 2372
	GTTCACCGGCGAYA	GGAATAGGATG	GTTCACCGGCGATATTCTGCGCGTGCCCGTGTCCGAGGACATGCTGGGCCGCAC
	TYCTGCG	GGTRATRTCGT	CTTCAACGGCAGCGGCATCCCCATCGACGGCGGCCCGCCCATCGTCGCAGAGAC
		CG	CTACCTCGACGTCCAGGGCATGCCGATTAATCCTCAAACGCGCATCTACCCGGAA
			GAAATGATCCAGACGGGGATCTCGACCATCGACGTGATGACGTCCATCGCGCGAG
			GGCAGAAGATCCCCATCTTCTCGGGCGCAGGGCTGCCACACAACGAGATCGCTG
			CGCAGATCTGCCGACAGGCGGGGCTGGTGCAGCACAAGGAGAACAAGGACGACT
			TCGCCATCGTGTTCGCGGCGATGGGCGTCAACATGGAGACGGCGCGCTTCTTCAA
			GCGCGAGTTCGCGCAGACGGGCGCGTGCAACGTGGTGCTGTTCCTCAACCTGGC
			CAACGACCCCACCATCGAGCGCATCATCACCCCGCGCCTCGCGCTCACCGTGGC
			CGAGTTCCTGGCCTACCAGTGCAACAAGCACGTGCTCGTCATCATGACCGACATG
			ACCTCCTACGCGGAGGCGCTGCGCGAGGTGAGCGCGGCGCGCGAGGAGGTTCC
			TGGGCGAAGAGGCTTCCCAGGCTACATGTACACCGATCTCTCCACCATCTACGAG
			CGCGCTGGCCGTGTGCAAGGCCGCCCCGGCTCCATCACTCAGATCCCCATCCTG
			ACGATGCCCAACGACGACATCACCCATCCTATTC

TABLE 3-LD

Target	cDNA SEQ
ID	ID NO	Corresponding amino acid sequence of cDNA clone

LD001

	1	SEQ ID NO: 2 (frame +1)
		GPKKHLKRLNAPKAWMLDKLGGVFAPRPSTGPHKLRESLPLVIFLRNRLKYALTNSEVTKIVMQRLIKVDGKVRTD
		SNYPAGFMDVITIEKTGEFFRLIYDVKGRFAVHRITAEEAKYKLCKVRRMQTGPKGIPFIVTHDGRTIR

LD002	3	SEQ ID NO: 4 (frame −3)
		AMQALKRKKRLEKNQLQIDGTLTTIELQREALEGASTNTTVLESMKNAAEALKKAHKNLDVDNVHDMMDDI

LD003	5	SEQ ID NO: 6 (frame −2)
		PRRPYEKARLDQELKIIGEYGLRNKREVWRVKYTLAKIRKAARELLTLEEKDQRRLFEGNALLRRLVRIGVLDETRM
		KLDYVLGLKIEDFLERRLQTQVFKLGLAKSIHHARVLVRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLKSPFGGGRP
		GRVKRKNL

LD006	7	SEQ ID NO: 8 (frame +1)
		HNYGWQVLVASGVVEYIDTLEEETVMIAMNPEDLRQDKEYAYCTTYTHCEIHPAMILGVCASIIPFPDHNQSPRNT
		YQSAMGKQAMGVYITNFHVRMDTLAHVLYYPHKPLVTTRSMEYLRFRELPAGINSIVAIACYTGYNQEDSVILNAS
		AVERGFFRSVFYRSYKDAESKRIGDQEEQFE

LD007	9	SEQ ID NO: 10 (frame +1)
		PKKDVKGTYVSIHSSGFRDFLLKPEILRAIVDCGFEHPSEVQHECIPQAVIGMDILCQAKSGMGKTAVFVLATLQQL
		EPADNVVYVLVMCHTRELAFQISKEYERFSKYMPSVKVGVFFGGMPIANDEEVLKNKCPHIVVGTPGRILALVKSR
		KLVLKNLKHFILDECDKMLELLDMRRDVQEIYRNTPHTKQVMMFSATLSKEIRPVCKKFMQDPMEVYVDDEAKLTL
		HGLQQHYVKLKENEKNKKLFELLDVLEFNQVVIFVKSVQRCVALAQLLTEQNFPAIGIHRGMDQKERLSRYEQFKD
		FQKRILVATNLFGRGMDIERVNIVFNYDMPEDSDTYLH

LD010	11	SEQ ID NO: 12 (frame +1)
		VKCSRELKIQGGIGSCVSLNVKNPLVSDTEIGMGNTVQWKMCTVTPSTTMALFFEVVNQHSAPIPQGGRGCIQFIT
		QYQHASGQKRIRVTTVARNWADASANIHHVSAGFDQEAAAVIMARMAVYRAESDDSPDVLRWVDRMLIRLCQKF
		GEYNKDDPNSFRLGENFSLYPQFMYHLRRSQFLQVFNNSPDETSFYRHMLMREDLTQSLIMIQPILYSYSFNGPP
		EPVLLDTSSIQPDRILLMDTFFQILIFHGETIAQW

LD011	13	SEQ ID NO: 14 (frame −1)
		PTFKCVLVGDGGTGKTTFVKRHMTGEFEKRYVATLGVEVHPLVFHTNRGPIRFNVWDTAGQEKFGGLRDGYYIQ
		GQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEK
		PFLWLARKLIGDPNLEFVAMPALLP

LD014
	15	SEQ ID NO: 16 (frame +3)
		QIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSSNMLNQARLKVLKV
		REDHVRTVLEEARKRLGQVTNDQGKYSQILESLILQGLYQLFEKDVTIRVRPQDRELVKSIIPTVTNKYKDATGKDI
		HLKIDDEIHLSQETTGGIDLLAQKNKIKISNTMEARLELISQQLLPEI

LD015	17	SEQ ID NO: 18 (frame −1)
		RHPSLFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEADKNSPAIIFI
		DELDAI

LD016	19	SEQ ID NO: 20 (frame −2)
		TVSGVNGPLVILEDVKFPKYNEIVQLKLADGTIRSGQVLEVSGSKAVVQVFEGTSGIDAKNTACEFTGDILRTPVSE
		DMLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGITAIDVMNSIARGQKIPIFSAAGLPHNEIAA
		QICRQAGLVKIPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALT
		AAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMP
		NDDITHPI

LD018	21	SEQ ID NO: 22 (frame +2)
		TWFKDGQRITESQKYESTFSNNQASLRVKQAQSEDSGHYTLLAENPQGCIVSSAYLAIEPVTTQEGLIHESTFKQQ
		QTEMEQIDTSKTLAPNFVRVCGDRDVTEGKMTRFDCRVTGRPYPDVTWYINGRQVTDDHNHKILVNESGNHALM
		ITTVSRNDSGVVTCVARNKTGETSFQCNLNVIEKEQVVAPKFVERFTTVNVAEGEPVSLRARAVGTPVPRITWQR
		DGAPLASGPDVRIAIDGGASTLNISRAKASDAAWYRC

LD027	23	SEQ ID NO: 24 (frame +1)
		HGGDKPYLISGADDRLVKIWDYQNKTCVQTLEGHAQNVTAVCFHPELPVALTGSEDGTVRVWHTNTHRLENCLN
		YGFERVWTICCLKGSNNVSLGYDEGSILVKVGREEPAVSMDASGGKIIWARHSELQQANLKALPEGGEIRDGERL
		PVSVKDMGACEIYPQTIQHNPNGRFVVVCGDGEYIIYTAMALRNKAFGSAQEFVWAQDSSEYAIRESGSTIRIFKN
		FKERKNFKSDFSAEGIYGGFLLGIKSVSGLTFYDWETLDLVRRIEIQPRAVYWSDSGKLVCLATEDSYFILSYDSEQ
		VQKARENNQVAEDGVEAAFDVLGEMNESVRTGLWVGDCFIYT

TABLE 3-PC

Target	cDNA SEQ
ID	ID NO	Corresponding amino acid sequence of cDNA clone

PC001	247	SEQ ID NO: 248 (frame +1)
		AWMLDKLGGVFAPRPSTGPHKLRESLPLVIFLRNRLKYALTNSEVTKIVMQRLIKVDGKVRTDSNYPAGFMDVITIE
		KTGEFFRLIYDVKGRFAVHRITAEEAKYKLCKVRRVQTGPKGIPFLVTHDGRTIRYPDPNIKVNDTIQMEIATSKILDY
		IKFES

PC003	249	SEQ ID NO: 250 (frame: +2)
		PRRPYEKARLDQELKIIGAFGLRNKREVWRVKYTLAKIRKAARELLTLEEKEPKRLFEGNALLRRLVRIGVLDENRM
		KLDYVLGLKIEDFLERRLQTQVFKSGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLKSPFGGGRP
		GRV

PC005	251	SEQ ID NO: 252 (frame +3)
		PNEINEIANTNSRQNIRKLIKDGLIIKKPVAVHSRARVRKNTEARRKGRHCGFGKRKGTANARMPQKELWVQRMR
		VLRRLLKKYREAKKIDRHLYHALYMKAKGNVFRNKRVLMEYIHKKKAEKARAKMLSDQANARRLKVKQARERRE

PC010	253	SEQ ID NO: 254 (frame +3)
		LKDSLQMSLSLLPPNALIGLITFGKMVQVHELGTEGCSKSYVFCGTKDLTAKQVQEMLGIGKGSPNPQQQPGQPG
		RPGQNPQAAPVPPGSRFLQPVSKCDMNLTDLIGELQKDPWPVHQGKRPLRSTGAALSIAVGLLECTYPNTGGRI
		MIFLGGPCSQGPGQVLNDDLKQPIRSHHDIHKDNAKYMKKAIKHYDHLAMRAATNSHCIDIYSCALDQTGLMEMK
		QCCNSTGGHMVMGDSFNSSLFKQTFQRVFSKDPKNDLKMAFNATLEVKCSRELKVQGGIGSCVSLNVKSPLVSD
		TELGMGNTVQWKLCTLAPSSTVALFFEVVNQHSAPIPQGGRGCIQLITQYQHASGQRRIRVTTIARNWADATANIH
		HISAGFDQEAAAVVMARMAGYKAESDETPDVLRWVDRMLIRLCQKFGEYNKDDPNSFRLGENFSLYPQFMYHLR
		RSQFLQVFNNSPDETSFYRHMLMREDLTQSLIMIQPILYSYSFNGPPEPVLLDTSSIQPDRILLMDTFFQILIFHGETI
		AQW

PC014	255	SEQ ID NO: 256 (frame +3)
		DVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSSNMLNQARLK
		VLKVREDHVRAVLEDARKSLGEVTKDQGKYSQILESLILQGLFQLFEKEVTVRVRPQDRDLVRSILPNVAAKYKDA
		TGKDILLKVDDESHLSQEITGGVDLLAQKNKIKISNTMEARLDLIA

PC016	257	SEQ ID NO: 258 (frame +2)
		LVILEDVKFPKFNEIVQLKLADGTLRSGQVLEVSGSKAVVQVFEGTSGIDAKNTVCEFTGDILRTPVSEDMLGRVFN
		GSGKPIDKGPPILAEDYLDIQGQPINPWSRIYPEEMIQTGITAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGL
		VKVPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAEFLAYQ
		CEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMP

PC027	259	SEQ ID NO: 260 (frame +1)
		QANLKVLPEGAEIRDGERLPVTVKDMGACEIYPQTIQHNPNGRFVVVCGDGEYIIYTAMALRNKAFGSAQEFVWA
		QDSSEYAIRESGSTIRIFKNFKEKKNFKSDFGAEGIYGGFLLGVKSVSGLAFYDWETLELVRRIEIQPRAIYWSDSG
		KLVCLATEDSYFILSYDSDQVQKARDNNQVAEDGVEAAFDVLGEINESVRTGLWVGDCFIYTNAVNRINYFVGGEL
		VTIAHLDRPLYVLGYVPRDDRLYLVDKELGVVSYXIAIICTRISDCSHATRLPNG*SSIAFNSK

TABLE 3-EV

	cDNA
Target	SEQ
ID	ID NO	Corresponding amino acid sequence of cDNA clone

EV005	513	SEQ ID NO: 514 (frame +3)
		RCGKKKVWLDPNEITEIANTNSRQNIRKLIKDGLIIKKPVAVHSRARVRKNTEARRKGRHCGFGKRKGTANARMPRK
		ELWIQRMRVLRRLLKKYREAKKIDRHLYHALYMKAKGNVFKNKRVMMDYIHKKKAEKARTKMLNDQADARRLKVKE
		ARKRREERIATKKQ

EV009	515	SEQ ID NO: 516 (frame +1)
		PTLDPSIPKYRTEESIIGTNPGMGFRPMPDNNEESTLIWLQGSNKTNYEKWKMNLLSYLDKYYTPGKIEKGNIPVKRC
		SYGEKLIRGQVCDVDVRKWEPCTPENHFDYLRNAPCIFLKLNRIYGWEPEYYNDPNDLPDDMPQQLKDHIRYNITNP
		VERNTVWVTCAGENPADVEYLGPVKYYPSFQGFPGYYFPYLNSEGYLSPLLAVQFKRPVSGIVINIECKAWA

EV010	517	SEQ ID NO: 518 (frame +3)
		GGHMVMGDSFNSSLFKQTFQRVFSKDSNGDLKMSFNAILEVKCSRELKVQGGIGPCVSLNVKNPLVSDLEIGMGNT
		VQWKLCSLSPSTTVALFFEVVNQHAAPIPQGGRGCIQFITQYQHSSGQKKIRVTTIARNWADATANIHHISAGFDEQT
		AAVLMARIAVYRAETDESSDVLRWVDRMLIRLCQKFGEYNKDDTNSFRLSENFSLYPQFMYHLRRSQFLQVFNNSP
		DETSFYRHMLMREDRNQ

EV015	519	SEQ ID NO: 520 (frame +1)
		RHPSLFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEADKNSPAIIFIDE
		LDAIAPKREKTHGEVERRIVSQLLTLMDGMKKSSHVIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHT
		KNMKLADDVDLEQIAAETHGHVGADLASLCSEAALQQIREKMDLIDLDDEQIDAEVLNSLAVTMENFRYAMSKSSPSA
		LRETV

EV016	521	SEQ ID NO: 522 (frame +2)
		TVSGVNGPLVILDSVKFPKFNEIVQLKLSDGTVRSGQVLEVSGQKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSED
		MLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQIC
		RQAGLVKIPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLTLTAAEFM
		AYQCEKHVLVILTDMSSYAEALREVSAA

TABLE 3-AG

	cDNA
Target	SEQ
ID	ID NO	Corresponding amino acid sequence of cDNA clone

AG001	601	SEQ ID NO: 602 (frame +1)
		HLKRFAAPKAWMLDKLGGVFAPRPSTGPHKLRESLPLVIFLRNRLKYALTNCEVTKIVMQRLIKVDGKVRTDPNYPAG
		FMDVITIEKTGEFFRLIYDVKGRFTIHRITAEEAKYKLCKVRKVQTGPKGIPFLVTHDGRTIRYPDPMIKVNDTIQLEIATS
		KILDFIKFESGNLCMITGGRNLGRVGTVVNRERHPGSFDIVHIRDANDHVFATRLNNVFVIGKGSKAFVSLPRGKGVK
		LSIA

AG005	603	SEQ ID NO: 604 (frame +2)
		VWLDPNEINEIANTNSRQNIRKLIKDGLIIKKPVAVHSRARVRKNTEARRKGRHCGFGKRKGTANARMPQKELWIQR
		MRVLRRLLKKYREAKKIDRHLYHALYMKAKGNVFKNKRVLMEYIHKKKAEKARAKMLADQANARRQKVKQVP*EEG
		RAYRREEAG

AG010	605	SEQ ID NO: 606 (frame +3)
		GGHMLMGDSFNSSLFKQTFQRVFAKDQNGHLKMAFNGTLEVKCSRELKVQGGIGSCVSLNVKSPLVADTEIGMGN
		TVQWKMCTFNPSTTMALFFEVVNQHSAPIPQGGRGCIQFITQYQHSSGQRRIRVTTIARNWADASANIHHISAGFDQ
		ERAAVIMARMAVYRAETDESPDVLRWVDRMLIRLCQKFGEYNKDDQASFRLGENFSLYPQFMYHLRRSQFLQVFNN
		SPDETSFYRHMLMREDLTQSLIMIQPILYSYSFNGPPEPVLLDTSSIQPDRILLMDTFFQILIFHGETIAQW

AG014	607	SEQ ID NO: 608 (frame +3)
		QIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSSNMLNQARLKVLKVRE
		DHVRAVLDEARKKLGEVTRDQGKYAQILESLILQGLYQLFEANVTVRVRPQDRTLVQSVLPTIATKYRDVTGRDVHLS
		IDDETQLSESVTGGIELLCKQNKIKVCNTLEARLDLISQQLVPQIRNALFGRNINRKF
AG016	609	SEQ ID NO: 610 (frame +1)

TABLE 3-TC

Target	cDNA SEQ
ID	ID NO	Corresponding amino acid sequence of cDNA clone

TC001	793	SEQ ID NO: 794 (frame +1)
		GPKKHLKRLNAPKAWMLDKLGGVFAPRPSTGPHKLRESLPLVIFLRNRLKYALTNSEVTKIVMQRLIKVDGKVRTD
		PNYPAGFMDVVTIEKTGEFFRLIYDVKGRFTIHRITGEEAKYKLCKVKKVQTGPKGIPFLVTRDGRTIRYPDPMIKVN
		DTIQLEIATSKILDFIKFESGNLCMITGGRNLGRVGTVVSRERHPGSFDIVHIKDANGHTFATRLNNVFIIGKGSKPYV
		SLPRGKGVKLSI

TC002	795	SEQ ID NO: 796 (frame +1)
		QEFLEAKIDQEILTAKKNASKNKRAAIQAIKRKKRYEKQLQQIDGTLSTIEMQREALEGANTNTAVLKTMKNAADAL
		KNAHLNMDVDEVHDMMDDI

TC010	797	SEQ ID NO: 798 (frame +3)
		PEVLVFGHVLVLEVPPLGDCLTVENQNLEKCVHEKDPIGLNGTSVEEDGFRGAVETITVQNRLDHNETLGEVLPH
		QHVAVERGLVWGVVENLEELGAAQMVHELGIETEVFTQTETVRVVFVVFAEF

TC014	799	SEQ ID NO: 800 (frame +1)
		EKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKPVELQKKIQSSNMLNQARLKVLKVREDHVHNVLDDARK
		RLGEITNDQARYSQLLESLILQSLYQYLGISDELFENNIVVRVRQQDRSIIQGILPVVATKYRDATGKDVHLKIDDES
		HLPSETTGGVVLYAQKGKIKIDNTLEARLDLIAQQLVPEIRTALFGRNINRKF

TC015	801	SEQ ID NO: 802 (frame +2)
		DELQLFRGDTVLLKGKRRKETVCIVLADENCPDEKIRMNRIVRNNLRVRLSDVVWIQPCPDVKYGKRIHVLPIDDTV
		EGLVGNLFEVYLKPYFLEAYRPIHKGDVFIVRGGMRAVEFKVVETEPSPYCIVAPDTVIHCDGDPIKREEEEEALNA
		VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKL
		AGESESNLRKAFEEADKNSPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGMKKSSHVIVMAATNRPNSIDPA
		LRRFGRFD

TABLE 3-MP

	cDNA
Target	SEQ ID
ID	NO	Corresponding amino acid sequence of cDNA clone

MP001	888	SEQ ID NO: 889 (frame +1)
		GPKKHLKRLNAPKAWMLDKSGGVFAPRPSTGPHKLRESLPLLIFLRNRLKYALTGAEVTKIVMQRLIKVDGKVRTDPN
		YPAGFMDVISIQKTSEHFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVQTGPKGVPFLTTHDGRTIRYPDPNIKVNDTIR
		YDIASSKILDHIRFETGNLCMITGGRNLGRVGIVTNRERHPGSFDIVHIKDANEHIFATRMNNVFIIGKGQKNYISLPRSK
		GVKLT

MP002	890	SEQ ID NO: 891 (frame +2)
		SFFSKVFGGKKEEKGPSTEDAIQKLRSTEEMLIKKQEFLEKKIEQEVAIAKKNGTTNKRAALQALKRKKRYEQQLAQID
		GTMLTIEQQREALEGANTNTAVLTTMKTAADALKSAHQNMNVDDVHDLMDDI

MP010	892	SEQ ID NO: 893 (frame +3)
		GCIQFITQYQHSSGYKRIRVTTLARNWADPVQNMMHVSAAFDQEASAVLMARMVVNRAETEDSPDVMRWADRTLI
		RLCQKFGDYQKDDPNSFRLPENFSLYPQFMYHLRRSQFLQVFNNSPDETSYYRHMLMREDVTQSLIMIQPILYSYSF
		NGRPEPVLLDTSSIQPDKILLMDTFFHILIFHGETIAQWRAMDYQNRPEYSNLKQLLQAPVDDAQEILKTRFPMPRYID
		TEQGGSQARFLLCKVNPSQTHNNMYAYGGWWSTSFDRCKLAAVHGAAA

MP016	894	SEQ ID NO: 895 (frame +1)
		VSEDMLGRVFNGSGKPIDKGPPILAEDYLDIEGQPINPYSRTYPQEMIQTGISAIDIMNSIARGQKIPIFSAAGLPHNEIA
		AQICRQAGLVKKPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALT
		AAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPN
		DDITHPI

MP027	896	SEQ ID NO: 897 (frame +3)
		PITKTRRVFRH*KAMLKIFLLVCFHPELPIVLTGSEDGTVRIWHSGTYRLESSLNYGLERVWTICCLRGSNNVALGYDE
		GSIMVKVGREEPAMSMDVHGGKIVWARHSEIQQANLKAMLQAEGAEIKDGERLPIQVKDMGSCEIYPQSISHNPNG
		RFLVVCGDGEYIIYTSMALRNKAFGSAQDFVWSSDSEYAIRENSSTIKVFKNFKEKKSFKPEGGADGIFGGYLLGVKS
		VTGLALYDWENGNLVRRIETQPKHVFWSESGELVCLATDEAYFILRFDVNVLSAARASNYEAASPDGLEDAFEILGEV
		QEVVKTGLWVGDCFIYTNGVNRINYYVGGEVVTVS

TABLE 3-NL

	cDNA SEQ
Target ID	ID NO	Corresponding amino acid sequence of cDNA clone

NL001	1071	SEQ ID NO: 1072 (frame +2)
		KSWMLDKLGGVYAPRPSTGPHKLRESLPLVIFLRNRLKYALTNCEVKKIVMQRLIKVDGKVRTDPNYPAGFMDVVQIEK
		TNEFFRLIYDVKGRFTIHRITAEEAKYKLCKVKRVQTGPKGIPFLTTHDGRTIRYPDPLVKVNDTIQLDIATSKIMDFIRFDS
		GNLCMITGGRNLGRVGTVVNRERHPGSFDIVHIKDVLGHTFATRLNNVFIIGKGSKAYVSLPKGKGVKLS

NL002	1073	SEQ ID NO: 1074 (frame +1)
		DEKGPTTGEAIQKLRETEEMLIKKQDFLEKKIEVEIGVARKNGTKNKRAAIQALKRKKRYEKQLQQIDGTLSTIEMQREAL
		EGANTNTAVLQTMKNAADALKAAHQHMDVDQ

NL003	1075	SEQ ID NO: 1076 (frame +2)
		PRRPYEKARLEQELKIIGEYGLRNKREVWRVKYALAKIRKAARELLTLEEKDQKRLFEGNALLRRLVRIGVLDEGRMKLD
		YVLGLKIEDFLERRLQTQVYKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFVVRLDSQKHIDFSLKSPFGGGRPGRV

NL004	1077	SEQ ID NO: 1078 (frame +1)
		KELAAVRTVCSHIENMLKGVTKGFLYKMRAVYAHFPINCVTTENNSVIEVRNFLGEKYIRRVRMAPGVTVTNSTKQKDEL
		IVEGNSIEDVSRSAALIQQSTTVKNKDIRKFLD

NL005	1079	SEQ ID NO: 1080 (frame +1)
		LDPNEINEIANTNSRQSIRKLIKDGLIIKKPVAVHSRARVRKNTEARRKGRHCGFGKRKGTANARMPQKVLWVNRMRVL
		RRLLKKYRQDKKIDRHLYHHLYMKAKGNVFKNKRVLMEFIHKKKAEKARMKMLNDQAEARRQKVKEAKKRRE

NL006	1081	SEQ ID NO: 1082 (frame +3)
		VLVSSGVVEYIDTLEEETTMIAMSPDDLRQDKEYAYCTTYTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAM
		GVYITNFHVRMDTLAHVLFYPHKPLVTTRSMEYLRFRELPAGINSVVAIACYTGYNQEDSVILNASAVERGFFRSVFFRS
		YKDAESKRIGDQEEQFEKPTRQTCQGMRNAIYDKLDDDGIIAPGLRVSGDDVVIGKTITLPDNDDELEGTTKRFTKRDAS
		TFLRNSETGIVDQVMLTLNSEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTSEGIAPDIIINPHAIPSR
		MTIGHLIECLQGKVSSNKGEIGDATPFN

NL007	1083	SEQ ID NO: 1084 (frame +2)
		FRDFLLKPEILRAILDCGFEHPSEVQHECIPQAVLGMDILCQAKSGMGKTAVFVLATLQQIEPTDNQVSVLVMCHTRELA
		FQISKEYERFSKCMPNIKVGVFFGGLPIQRDEETLKLNCPHIVVGTPGRILALVRNKKLDLKHLKHFVLDECDKMLELLDM
		RRDVQEIFRNTPHSKQVMMFSATLSKEIRPVCKKFMQDPMEVYVDDEAKLTLHGLQQHYVKLKENEKNKKLFELLDILE
		FNQVVIFVKSVQRCMALSQLLTEQNFPAVAIHRGMTQEERLKKYQEFKEFLKRILVATNLFGRGMDIERVNIVFNYDMP

NL008	1085	SEQ ID NO: 1086 (frame +1)
		GRIENQKRVVGVLLGCWRPGGVLDVSNSFAVPFDEDDKEKNVWFLDHDYLENMFGMFKKVNAREKVVGWYHTGPKL
		HQNDVAINELIRRYCPNCVLVIIDAKPKDLGLPTEAYRVVEEIHDDGSPTSKTFEHVMSEIGAEEAEEIGVEHLLRDIKDTT
		VGSLSQRVTNQLMGLKGLHLQLQDMRDYLNQVVEGKLPMNHQIVYQLQDIFNLLPDIGHGNFVDSLY

NL009	1087	SEQ ID NO: 1088 (frame +1)
		CDYDRPPGRGQVCDVDVKNWFPCTSENNFNYHQSSPCVFLKLNKIIGWQPEYYNETEGFPDNMPGDLKRHIAQQKSI
		NKLFMQTIWITCEGEGPLDKENAGEIQYIPRQGFPGYFYPYTNA

NL010	1089	SEQ ID NO: 1090 (amino terminus end) (frame +2)
		SSRLEATRLVVPVGCLYQPLKERPDLPPVQYDPVLCTRNTCRAILNPLCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQ
		HQPAELIPSFSTIEYIITRAQTMPPMFVLVVDTCLDDEELGALKDSLQMSLSLLPPNALIGLITFGKMVQVHELGCDGCSK
		SYVFRGVKDLTAKQIQDMLGIGKMAAAPQPMQQRIPGAAPSAPVNRFLQPVGKCDMSLTDLLGELQRDPWNVAQGKR
		PLRSTGVALSIAVGLLECT

	1115	SEQ ID NO: 1116 (carboxy terminus end) (frame +3)
		LNVKGSCVSDTDIGLGGTSQWKMCAFTPHTTCAFFFEVVNQHAAPIPQGGRGCIQFITQYQHSSGQRRIRVTTIARNWA
		DASTNLAHISAGFDQEAGAVLMARMVVHRAETDDGPDVMRWADRMLIRLCQRFGEYSKDDPNSFRLPENFTLYPQFM
		YHLRRSQFLQVFNNSPDETSYYRHILMREDLTQSLIMIQPILYSYSFNGPPEPVLLDTSSIQPDRILLMDTFFQILIFHGETIA

NL011	1091	SEQ ID NO: 1092 (frame +2)
		DGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGVIRFNVWDTAGQEKFGGLRDGYYIQGQCAIIMFDVTSRV
		TYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLAKKLIGDPNLEFV
		AMPALLPPEVTMDPQX

NL012	1093	SEQ ID NO: 1094 (frame +2)
		QQTQAQVDEVVDIMKTNVEKVLERDQKLSELDDRADALQQGASQFEQQAGKLKRKF

NL013	1095	SEQ ID NO: 1096 (frame +2)
		AEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGR
		PEMVVGWYHSHPGFGCWLSGVDINTQESFEQLSKRAVAVVV

NL014	1097	SEQ ID NO: 1098 (frame +2)
		FIEQEANEKAEEIDAKAEEEFNIEKGRLVQHQRLKIMEYYDRKEKQVELQKKIQSSNMLNQARLKALKVREDHVRSVLEE
		SRKRLGEVTRNPAKYKEVLQYLIVQGLLQLLESNVVLRVR
		EADVSLIEGIVGSCAEQYAKMTGKEVVVKLDADNFLAAETCGGVELFARNGRIKIPNTLESRLDLISQQLVPEIRVALF

NL015	1099	SEQ ID NO: 1100 (frame +1)
		IVLSDETCPFEKIRMNRVVRKNLRVRLSDIVSIQPCPDVKYGKRIHVLPIDDTVEGLTGNLFEVYLKPYFLEAYRPIHKDDA
		FIVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGDPIKREDEEDAANAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFK
		AIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEADKNAPAIIFIDELDAIAPKRE
		KTHGEVERRIVSQLLTLMDGLKQSSHVIVMAATNRPNSIDAALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMKLADDVD
		LEX

NL016	1101	SEQ ID NO: 1102 (frame +2)
		TPVSEDMLGRVFNGSGKPIDKGPPILAEDYLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIA
		AQICRQAGLVKLPGKSVLDDSEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAE
		FLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSIT

NL018	1103	SEQ ID NO: 1104 (frame +2)
		MQMPVPRPQIESTQQFIRSEKTTYSNGFTTIEEDFKVDTFEYRLLREVSFRESLIRNYLHEADMQMSTVVDRALGPPSAP
		HIQQKPRNSKIQEGGDAVFSIKLSANPKPRLVWFKNGQRIGQTQKHQASYSNQTATLKVNKVSAQDSGHYTLLAENPQ
		GCTVSSAYLAVESAGTQDTGYSEQYSRQEVETTEAVDSSKMLAPNFVRVPADRDASEGKMTRFDCRVTGRPYPDVA
		WFINGQQVADDATHKILVNESGNHSLMITGVTRLDHGVVGCIARNKAGETSFQCNLNVIEKELVVAPKFVERFAQVNVK
		EGEPVVLSARAVGTPVPRITWQKDGAPIQSGPSVSLFVDGGATSLDIPYAKAS

NL019	1105	SEQ ID NO: 1106 (frame +2)
		DDTYTESYISTIGVDFKIRTIDLDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDCTDQESFNNLKQWLEEIDRYAC
		DNVNKLLVGNKCDQTNKKVVDYTQAKEYADQLGIPFLETSAKNATNVEQAF

NL021	1107	SEQ ID NO: 1108 (frame +2)
		VSLNSVTDISTTFILKPQENVKITLEGAQACFISHERLVISLKGGELYVLTLYSDSMRSVRSFHLEKAAASVLTTCICVCEE
		NYLFLGSRLGNSLLLRFTEKELNLIEPRAIESSQSQNPAKKKKLDTLGDWMASDVTEIRDLDELEVYGSETQTSMQIASYIF

NL022	1109	SEQ ID NO: 1110 (frame +2)
		TLHREFLSEPDLQSYSVMIIDEAHERTLHTDILFGLVKDVARFRPDLKLLISSATLDAQKFSEFFDDAPIFRIPGRRFPVDIY
		YTKAPEADYVDACVVSILQIHATQPLGDILVFLTGQEEIETCQELLQDRVRRLGPRIKELLILPVYSNLPSDMQAKIFLPTPP
		NARKVVLATNIAETSLTIDNIIYVIDPGFCKQNNFNSRTGMESLVVVPVSKASANQRAGRAGRVAAGKCFRLYT

NL023	1111	SEQ ID NO: 1112 (frame +2)
		RSFSQERQHEEMKESSGRMHHSDPLIVETHSGHVRGISKTVLGREVHVFTGIPFAKPPIGPLRFRKPVPVDPWHGVLDA
		TALPNSCYQERYEYFPGFEGEEMWNPNTNLSEDCLYLNIWVPHRLRIRHRANSEENKPRAKVPVLIWIYGGGYMSGTA
		TLDVYDADMVAATSDVIVASMQYRVGAFGFLYLAQDLPRGSEEAPGNMGLWDQALAIRWLKDNIAAFGGDPELMTLFG
		ESAGGGSVSIHLVSPITRGLARRGIMQSGTMNAPWSFMTAERATEIAKTLIDDCGCNSSLLTDAPSRVMSCMRSVEAKII
		SVQQWNSYSGILGLPSAPTIDGIFLPKHPLDLLKEGDFQDTEILIGSNQDEGTYFILYDFIDFFQKDGPSFLQRDKFLDIINT
		IFKNMTKIEREAIIFQYTDWEHVMDGYLNQKMIGDVVGDYFFICPTNHFAQAFAEHGKKVYYYFFTQRTSTSLWGEWMG
		VMHGDEIEYVFGHPLNMSLQFNARERDLSLRIMQAYSRFALTGKPVPDDVNWPIYSKDQPQYYIFNAETSGTGRGPRA
		TACAF

NL027	1113	SEQ ID NO: 1114 (frame +2)
		PIVLTGSEDGTVRIWHSGTYRLESSLNYGLERVWTICCMRGSNNVALGYDEGSIMVKVGREEPAISMDVNGEKIVWARH
		SEIQQVNLKAMPEGVEIKDGERLPVAVKDMGSCEIYPQTIAHNPNGRFLVVCGDGEYIIHTSMVLRNKAFGSAQEFIWG
		QDSSEYAIREGTSTVKVFKNFKEKKSFKPEFGAESIFGGYLLGVCSLSGLALYDWETLELVRRIEIQPKHVYWSESGELV
		ALATDDSYFVLRYDAQAVLAARDAGDDAVTPDGVEDAFEVLGEVHETVKTGLWVGDCFIYT

TABLE 3-CS

Target	cDNA
ID	SEQ ID NO	Corresponding amino acid sequence of cDNA clone

CS001	1682	SEQ ID NO: 1683 (frame +1)
		KAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTYPAGFMDVV
		SIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVRRVATGPKNVPYLVTHDGRTVRYPDPLIKVNDSIQLDIATSK
		IMDFIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIRDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGVR
		LT

CS002	1684	SEQ ID NO: 1685 (frame +1)
		SFFSKVFGGKKEEKGPSTHEAIQKLRETEELLQKKQEFLERKIDTELQTARKHGTKNKRAAIAALKRKKRYEKQLT
		QIDGTLTQIEAQREALEGANTNTQVLNTMRDAATAMRLAHKDIDVDKVHDLMDDI

CS003	1686	SEQ ID NO: 1687 (frame +1)
		GLRNKREVWRVKYTLARIRKAARELLTLEEKDPKRLFEGNALLRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLQ
		TQVFKAGLAKSIHHARILIRQRHIRVRKQVVNIPSFIVRLDSGKHIDFSLKSPFGGGRP

CS006	1688	SEQ ID NO: 1689 (frame +1)
		TCQGMRNALYDKLDDDGIIAPGIRVSGDDVVIGKTITLPENDDELEGTSRRYSKRDASTFLRNSETGIVDQVMLTL
		NSEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGLTPDIIINPHAIPSRMTIGHLIECIQGK
		VSSNKGEIGDATPFNDAVNVQKI

CS007	1690	SEQ ID NO: 1691 (frame +3)
		SEISCWNQRFWGLSSIAVSSTLQKFNMNVFPKLFWEWIFFVKAKSGMGKTAVFVLATLQQLEPSENHVYVLVMC
		HTRELAFQISKEYERFSKYMAGVRVSVFFGGMPIQKDEEVLKTACPHIVVGTPGRILALVNNKKLNLKHLKHFILD
		ECDKMLESLDMRRDVQEIFRNTPHGKQVMMFSATLSKEIRPVCKKFMQDPMEVYVDDEAKLTLHGLQQHYVKL
		KENEKNKKLFELLDVLEFNQVVIFVKSVQRCIALAQLLTDQNFPAIGIHRNMTQDERLSRYQQFKDFQKRILVATN
		LFGRGMDIERVNIVFNYDMP

CS009	1692	SEQ ID NO: 1693 (frame +1)
		LVAICIWTFLQRLDSREPMWQLDESIIGTNPGLGFRPTPPEVASSVIWYKGNDPNSQQFWVQETSNFLTAYKRD
		GKKAGAGQNIHNCDFKLPPPAGKVCDVDISAWSPCVEDKHFGYHKSTPCIFLKLNKIFGWRPHFYNSSDSLPTD
		MPDDLKEHIRNMTAYDKNYLNMVWVSCEGENP

CS011	1694	SEQ ID NO: 1695 (frame +1)
		GSGKTTFVKRHLTGEFEKRYVATLGVEVHPLVFHTNRGPIRFNVWDTAGQEKFGGLRDGYYIQGQCAIIMFDVT
		SRVTYKNVPNWHRDLVRVCEGIPIVLCGNKVDIKDRKVKAKTIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLI
		GDGNLEFVAMQPCFH

CS013	1696	SEQ ID NO: 1697 (frame +2)
		DAPVVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQTGTGVSVEAVDPVFQA
		KMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKG

CS014	1698	SEQ ID NO: 1699 (frame +2)
		QKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSSNMLNQARLKV
		LKVREDHVRNVLDEARKRLAEVPKDVKLYTDLLVTLVVQALFQLMEPTVTVRVRQADVSLVQSILGKAQQDYKA
		KIKKDVQLKIDTENSLPADTCGGVELIAARGRIKISNTLESRLELIAQQLLPEIRTALF

CS015	1700	SEQ ID NO: 1701 (frame +1)
		IVLSDDNCPDEKIRMNRVVRNNLRVRLSDIVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLFEVYLKPYFMEAYRPI
		HRDDTFMVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGDPIKREEEEEALNAVGYDDIGGCRKQLAQIKEMV
		ELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEADKN
		SPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGMKKSSHVIVMAATNRPNSIDPAL

CS016	1702	SEQ ID NO: 1703(frame −3)
		TPVSEDMLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLP
		HNEIAAQICRQAGLVKIPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERII
		TPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSI
		TQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPVNVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYAC
		YAIGKDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFITQGNYENRTVFESLDIGWQLLRIFPKEMLKRIPASI

CS018	1704	SEQ ID NO: 1705 (frame +2)
		SVYIQPEGVPVPAQQSQQQQSYRHVSESVEHKSYGTQGYTTSEQTKQTQKVAYTNGSDYSSTDDFKVDTFEY
		RLLREVSFRESITKRYIGETDIQISTEVDKSLGVVTPPKIAQKPRNSKLQEGADAQFQVQLSGNPRPRVSWFKNG
		QRIVNSNKHEIVTTHNQTILRVRNTQKSDTGNYTLLAENPNGCVVTSAYLAVESPQETYGQDHKSQYIMDNQQT
		AVEERVEVNEKALAPQFVRVCQDRDVTEGKMTRFDCRVTGRPYPEVTWFINDRQIRDDYXHKILVNESCNHAL
		MITNVDLSDSGVVSCIARNKTGETSFQCRLNVIEKEQVVAPKFVERFSTLNVREGEPVQLHARAVGTPTPRITWQ
		KDGVQVIPNPELRINTEGGASTLDIPRAKASDAGWYRC

TABLE 3-PX

	cDNA
Target	SEQ ID
ID	NO	Corresponding amino acid sequence of cDNA clone

PX001	2100	SEQ ID NO: 2101 (frame +1)
		GPKKHLKRLNAPRAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLQPPQVRAQRQRGAEDREAAPHQGGRQGPH
		RPHLPGWIHGCCVDKDQAVPSDLRCEGTLHHPPHHSRGGQVQAVQGEARGDGPQERAVHRDAQRPHAALPRP
		AHQGQRLHPARHRHLQDHGHHQVRLRPVHDHGRALGASGHHRVPREAPRELRHRPHQGHHRTHLRHQVEQRV
		HHRQGHE

PX009	2102	SEQ ID NO: 2103 (frame +3)
		TLIWYKGTGYDSYKYWENQLIDFLSVYKKKGQTAGAGQNIFNCDFRNPPPHGKVCDVDIRGWEPCIDENHFSFHKS
		SPCIFLKLNKIYGWRPEFYNDTANLPEAMPVDLQTHIRNITAFNRDYANMVWVSCHGETPADKENIGPVRYLPYPGFP
		GYFYPYENAEGYLSPLVAVHLERPRTGIVINIECKAWA

PX010	2104	SEQ ID NO: 2105 (frame +3)
		GCIQFITQYQHSSGQRRVRVTTVARNWGDAAANLHHISAGFDQEAAAVVMARLVVYRAEQEDGPDVLRWLDRMLIR
		LCQKFGEYAKDDPNSFRLSENFSLYPQFMYHLRRSQFLQVFNNSPDETTFYRHMLMREDLTQSLIMIQPILYSYSFG
		GAPEPVLLDTSSIQPDRILLMDTFFQILIYHGETMAQWRALRYQDMAEYENFKQLLRAPVDDAQEILQTRFPVPRYIDT
		EHGGSQARFLLSKVNPSQTHNNMYAYGGAMPIPSADGGAPVLTDDVSLQVFMEQP

PX015	2106	SEQ ID NO: 2107 (frame +3)
		RKETVCIVLSDDNCPDEKIRMNRVVRNNLRVRLSDIVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLFEVYLKPYFMEA
		YRPIHRDDTFMVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREEEEEALNAVGYDDIGGCRKQLAQIKEMV
		ELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEADKNSPAI
		ILIDELDAI

PX016	2108	SEQ ID NO: 2109 (frame +2)
		FTGDILRTPVSEDMLGRIFNGSGKPIDKGPPILAEEYLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSA
		AGLPHNEIAAQICRQAGLVKVPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIE
		RIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSIT
		QIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPVNVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYACYAIG
		KDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFITQGSYENRTVFESLDIGWQPLRIFPKEM

TABLE 3-AD

	cDNA
Target	SEQ ID
ID	NO	Corresponding amino acid sequence of cDNA clone

AD001	2364	SEQ ID NO: 2365 (frame +1)
		GPKKHLKRLNAPKAWMLDKLGGVFAPRPSTGPHKLRECLPLVIFLRNRLKYALTNCEVTKIVMQRLIKVDGKVRTDPN
		YPAGFMDVVTIEKTGEFFRLVYDVKGRFTIHRISAEEAKYKLCKVRRVQTGPKGIPFLVTHDGRTIRYPDPVIKVNDSI
		QLDIATCKIMDHIRFESGNLCMITGGRNLGRVGTVVSRERHPGSFDIVHIKDTQGHTFATRLNNVFIIGKATKPYISLPK
		GKGVKLSIAEERDK

AD002	2366	SEQ ID NO: 2367 (frame +2)
		SFFSKVFGGKKDGKAPTTGEAIQKLRETEEMLIKKQEFLEKKIEQEINVAKKNGTKNKRAAIQALKRKKRYEKQLQQID
		GTLSTIEMQREALEGANTNTAVLQTMKSAADALKAAHQHMDVDKVHDLMDDI

AD009	2368	SEQ ID NO: 2369 (frame +3)
		VLAALVAVCLWVFFQTLDPRIPTWQLDSSIIGTSPGLGFRPMPEDSNVESTLIWYRGTDRDDFRQWTDTLDEFLAVY
		KTPGLTPGRGQNIHNCDYDKPPKKGQVCNVDIKNWHPCIQENHYNYHKSSPCIFIKLNKIYNWIPEYYNESTNLPEQM
		PEDLKQYIHNLESNNSREMNTVWVSCEGENP

AD015	2370	SEQ ID NO: 2371 (frame +2)
		DELQLFRGDTVLLKGKRRKETVCIVLSDDTCPDGKIRMNRVVRNNLRVRLSDVVSVQPCPDVKYGKRIHVLPIDDTVE
		GLTGNLFEVYLKPYFLEAYRPIHKDDAFIVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGDPIKREEEEEALNAVGY
		DDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESE
		SNLRKAFEEADKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQSSHVIVMAATNRPNSIDGALRRFGRF
		DREIDIGIPDATGRLEILRIHTKNMKLADDVDLEQIAAESHG

AD016	2372	SEQ ID NO: 2373 (frame +2)
		FTGDILRVPVSEDMLGRTFNGSGIPIDGGPPIVAETYLDVQGMPINPQTRIYPEEMIQTGISTIDVMTSIARGQKIPIFSG
		AGLPHNEIAAQICRQAGLVQHKENKDDFAIVFAAMGVNMETARFFKREFAQTGACNVVLFLNLANDPTIERIITPRLAL
		TVAEFLAYQCNKHVLVIMTDMTSYAEALREVSAAREEVPGRRGFPGYMYTDLSTIYERAGRVQGRPGSITQIPILTMP
		NDDITHPI

TABLE 4-LD

Target ID	SEQ ID NO	Sequences*	Example Gi-number and species

LD001	49	GGCCCCAAGAAGC	3101175 (Drosophila melanogaster), 92477283 (Drosophila
		ATTTGAAGCGTTT	erecta)

LD001	50	AATGCCCCAAAAGCAT	70909480 (Carabus granulatus), 77325294 (Chironomus tentans),
		GGATGTTGGATAAA	900945 (Ctenocephalides felis), 60297219 (Diaprepes
		TTGGGAGGTGT	abbreviatus), 37951951 (Ips pini), 75735533 (Tribolium
			castaneum),
			22039624 (Ctenocephalides felis)

LD001	51	GAAGTTACTAAG	33368080 (Glossina morsitans)
		ATTGTTATGCA

LD001	52	ATTGAAAAAACTGG	60297219 (Diaprepes abbreviatus)
		TGAATTTTTCCG

LD001	53	ACACACGACGGCC	27555937 (Anopheles gambiae), 33355008 (Drosophila yakuba),
		GCACCATCCGCT	22474232 (Helicoverpa armigera), 3738704 (Manduca sexta)

LD001	54	ACACACGACGGCC	92477283 (Drosophila erecta)
		GCACCATCCGCTA

LD001	55	CCCAAGAAGCATTT	92954810 (Drosophila ananassae), 92231605 ( Drosophila
		GAAGCGTTTG	willistoni)

LD002	56	GCAATGTCATCCAT	17861597 (Drosophila melanogaster), 92223378 ( Drosophila
		CATGTCGTG	willistoni), 92471309 (Drosophila erecta)

LD003	57	CAGGTTCTTCCTCTT	24975810 (Anopheles gambiae), 3478578 (Antheraea yamamai),
		GACGCGTCCAGG	42764756 (Armigeres subalbatus), 24661714 (Drosophila
			melanogaster), 68267151 (Drosophila simulans), 33355000
			(Drosophila yakuba), 49532931 (Plutella xylostella), 76552910
			(Spodoptera frugiperda), 92959651 (Drosophila ananassae),
			92467993 (Drosophila erecta)

LD003	58	TTGAGCGAGAAGTC	49558930 (Boophilus microplus)
		AATATGCTTCT

LD003	59	TTCCAAGAAATCTTC	62238687 (Diabrotica virgifera), 76169907 (Diploptera punctata),
		AATCTTCAAACCCAA	67872253 (Drosophila pseudoobscura), 55877642 (Locusta
			migratoria), 66548956 (Apis mellifera)

LD003	60	TTCATCCAACACTCC	22040140 (Ctenocephalides felis)
		AATACG

LD003	61	AAGAGCATTGCCTTC	2459311 (Antheraea yamamai)
		AAACAACCT

LD003	62	AGTTCTCTGGCAGCTT	76169907 (Diploptera punctata)
		TACGGATTTT

LD003	63	CCACACTTCACGTTTG	57963694 (Heliconius melpomene)
		TTCCT

LD003	64	CCGTATGAAGCTTGATT	108742527 (Gryllus rubens), 108742525 (Gryllus pennsylvanicus),
		ACGT	108742523 (Gryllus veletis), 108742521 (Gryllus bimaculatus),
			108742519 (Gryllus firmus), 109194897 (Myzus persicae)

LD003	65	AGGAACAAACGTGAA	109194897 (Myzus persicae)
		GTGTGGCG

LD006	66	AGCGCTATGGGTAAG	27819970 (Drosophila melanogaster)
		CAAGCTATGGG

LD006	67	TGTTATACTGGTTATAA	55801622 (Acyrthosiphon pisum), 66535130 (Apis mellifera)
		TCAAGAAGAT

LD007	68	GAAGTTCAGCACGAAT	50563603 (Homalodisca coagulata)
		GTATTCC

LD007	69	CAAGCAAGTGATGATG	50563603 (Homalodisca coagulata)
		TTCAGTGCCAC

LD007	70	TGCAAGAAATTCATGC	21068658 (Chironomus tentans)
		AAGATCC

LD007	71	AAATGAAAAGAAT	49201437 (Drosophila melanogaster)
		AAAAAATT

LD007	72	CAGAATTTCCCAGCC	67895225 (Drosophila pseudoobscura)
		ATAGGAAT

LD007	73	AGCAGTTCAAAGATTT	77848709 (Aedes aegypti)
		CCAGAAG

LD007	74	TTCCAAATCAGCAAAG	91083250 (Tribolium castaneum)
		AGTACGAG

LD010	75	TACCCGCAGTTCATGT	29558345 (Bombyx mori)
		ACCAT

LD010	76	CAGTCGCTGATCATG	49559866 (Boophilus microplus)
		ATCCAGCC

LD010	77	CTCATGGACACGTT	60293559 (Homalodisca coagulata)
		CTTCCAGAT

LD010	78	GGGGCTGCATACAG	92971011 (Drosophila mojavensis)
		TTCATCAC

LD010	79	CCCGCAGTTCATGT	92952825 (Drosophila ananassae)
		ACCATTTG

LD010	80	GACAATGCCAAATA	92921253 (Drosophila virilis)
CATGAAGAA

LD010	81	TTCGATCAGGAGG	92921253 (Drosophila virilis)
		CAGCCGCAGTG

LD011	82	AGCAGGGCTGGC	28317118 (Drosophila melanogaster)
		ATGGCGACAAA

LD011	83	TTCTCAAAGTTGTA	37951963 (Ips pini)
		GTTAGATTTGGC

LD011	84	TACTGCAAATTCTT	55883846 (Locusta migratoria)
		CTTCCTATG

LD011	85	GGTACATTCTTGTA	67885713 (Drosophila pseudoobscura)
		TGTAACTC

LD011	86	TCAAACATGATAAT	68771114 (Acanthoscurria gomesiana)
		AGCACACTG

LD011	87	TCTCCTGACCGGC	17944197 (Drosophila melanogaster), 77843537 (Aedes aegypti),
		AGTGTCCCATA	94469127 (Aedes aegypti), 24664595 (Drosophila melanogaster)

LD011	88	GCTACTTTGGGAG	101410627 (Plodia interpuntella)
		TTGAAGTCCATCC

LD011	89	TAACTACAACTTT	90813103 (Nasonia vitripennis)
		GAGAAGCCTTTCCT

LD011	90	AAGTTTGGTGGT	84267747 (Aedes aegypti)
		CTCCGTGATGG

LD014	91	GCAGATCAAGCA	9732 (Manduca sexta), 90814338 (Nasonia vitripennis), 87266590
		TATGATGGC	(Choristoneura fumiferana)

LD014	92	ATCAAGCATATGAT	75470953 (Tribolium castaneum), 76169390 (Diploptera punctata)
		GGCTTTCATTGA

LD014	93	AATATTGAAAAGGGG	78055682 (Heliconius erato)
		CGCCTTGT

LD014	94	CAACGTCTCAAGATT	37659584 (Bombyx mori)
		ATGGAATA

LD014	95	ATTATGGAATATTAT	66556286 (Apis mellifera)
		GAGAAGAAAGA

LD014	96	AACAAAATCAAGAT	25958976 (Curculio glandium)
		CAGCAATACT

LD016	97	ATGTCGTCGTTGGG	27372076 (Spodoptera littoralis)
		CATAGTCA

LD016	98	GTAGCTAAATCGGT	27372076 (Spodoptera littoralis), 55797015 (Acyrthosiphon
		GTACATGTAACCTGGG	pisum), 73615307 (Aphis gossypii), 4680479 (Aedes aegypti),
		AAACCACGACG	9713 (Manduca sexta), 76555122 (Spodoptera frugiperda),
			237458 (Heliothis virescens), 53883819 (Plutella xylostella),
			22038926 (Ctenocephalides felis), 101403557 (Plodia
			interpuntella), 92969578 (Drosophila grimshawi), 91829127
			(Bombyx mori)

LD016	99	GCAGATACCTCACGC	62239897 (Diabrotica virgifera)
		AAAGCTTC

LD016	100	GGATCGTTGGCCAAA	67882712 (Drosophila pseudoobscura), 92985459 (Drosophila
		TTCAAGAACAGGCA	grimshawi)

LD016	101	TTCTCCATAGAACCGTT	4680479 (Aedes aegypti), 27372076 (Spodoptera littoralis)
		CTCTTCGAAATCCTG

LD016	102	GCTGTTTCCATGTTAAC	49558344 (Boophilus microplus)
		ACCCAT

LD016	103	TCCATGTTAACACCCAT	62238871 (Diabrotica virgifera)
		AGCAGCGA

LD016	104	CTACAGATCTGGGCAG	22038926 (Ctenocephalides felis), 16898595 (Ctenocephalides
		CAATTTCATTGTG	felis)

LD016	105	GGCAGACCAGCTGCA	22038926 (Ctenocephalides felis), 16898595 (Ctenocephalides
		GAGAAAAT	felis)

LD016	106	GAGAAAATGGGGATC	4680479 (Aedes aegypti), 9713 (Manduca sexta),
		TTCTGACCACGAGCA	22038926 (Ctenocephalides felis), 16898595 (Ctenocephalides
		ATGGAGTTCATCACGTC	felis), 67877903 (Drosophila pseudoobscura), 10763875
			(Manduca sexta), 76554661 (Spodoptera frugiperda), 77905105
			(Aedes aegypti),
			50562965 (Homalodisca coagulata), 27372076 (Spodoptera
			littoralis)

LD016	107	ATGGAGTTCATCACGT	9713 (Manduca sexta), 237458 (Heliothis virescens),
		CAATAGC	76554661 (Spodoptera frugiperda), 22474331 (Helicoverpa
			armigera)

LD016	108	GTCTGGATCATTTCCT	16898595 (Ctenocephalides felis),
		CAGGATAGATACGG	22038926 (Ctenocephalides felis),
		GACCACGGATTGAT	50562965 (Homalodisca coagulata),
		TGGTTGACCCTGGA	49395165 (Drosophila melanogaster),
		TGTCCAAGAAGTCTT	6901845 (Bombyx mori), 92931000 (Drosophila virilis)
		CAGCCAAAATTGGGG
		GACCTTTGTC

LD016	109	ATTGGGGGACCTTTGT	10763875 (Manduca sexta)
		CGATGGG

LD016	110	ATGGGTTTTCCTGAT	49395165 (Drosophila melanogaster),
		CCATTGAAAACACGTCCC	55905051 (Locusta migratoria)
		AACATATCTTCAGAAA
		CAGGAGTCCTCAAAA
		TATCTCCTGTGAATTC
		ACAAGCGGTGTTTTT
		GGCGTCGATTCCTGA
		TGTGCCCTCGAACAC
		TTGAACCACAGCTTT

LD016	111	ACAGCTTTTGACCCAC	21642266 (Amblyomma variegatum)
		TGACTTCCAG

LD016	112	GACCCACTGACTTCCA	49395165 (Drosophila melanogaster)
		GAACTTGTCCCGAA
		CGTATAGTGCCATCA
		GCCAGTTTGAGT

LD016	113	GGACCGTTCACACCAG	24646342 (Drosophila melanogaster)
		ACACAGT

LD016	114	GACTGTGTCTGGTGTG	103769163 (Drosophila melanogaster), 92048971 (Drosophila
		AACGGTCCTCT	willistoni)

LD016	115	TTCTCTTCGAAATCCTG	84116133 (Dermatophagoides farinae)
		TTTGAA

LD016	116	GACTGTGTVTGGTGTG	24646342 (Drosophila melanogaster)
		AACGGTCC

LD016	117	GGTCGTCGTGGTTT	92231646 (Drosophila willistoni), 91755555 (Bombyx mori),
		CCCAGGTTACATGTAC	84228226 (Aedes aegypti)
		ACCGATTT

LD016	118	TGACAGCTGCCGAA	92231646 (Drosophila willistoni)
		TTCTTGGC

LD018	119	CAAGTCACCGACGA	91080016 (Tribolium castaneum)
		CCACAACCACAA

LD018	120	ATCGCGATTGACGG	91080016 (Tribolium castaneum)
		TGGAGCC

LD027	121	AGACGATCGGTTGG	66501387 (Apis mellifera)
		TTAAAATC

LD027	122	GATATGGGAGCATG	77326476 (Chironomus tentans)
		TGAAATATA

LD027	123	TTAGAGAATTGTTTG	90129719 (Bicyclus anynana)

TABLE 4-PC

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

PC001	275	AAAATTGTCATGCAAAGGTTGAT	37952206 (Ips pini)

PC001	276	AAAGCATGGATGTTGGACAAA	98994282 (Antheraea mylitta)
			109978109 (Gryllus pennsylvanicus)
			55904580 (Locusta migratoria)

PC001	277	AAAGCATGGATGTTGGACAAATT	31366663 (Toxoptera citricida)

PC001	278	AAAGCATGGATGTTGGACAAATTGGG	60311985 (Papilio dardanus)

PC001	279	AAAGCATGGATGTTGGACAAATTGGGGGGTGT	37951951 (Ips pini)

PC001	280	AAATACAAGTTGTGTAAAGTAA	84647793 (Myzus persicae)

PC001	281	AAGCATGGATGTTGGACAAATTGGGGGGTGT	70909486 (Mycetophagus quadripustulatus)

PC001	282	ATGGATGTCATTACTATTGAGAA	25957367 (Carabus granulatus)

PC001	283	CATCAAATTTGAATCTGGCAACCT	37952206 (Ips pini)

PC001	284	CATGATGGCAGAACCATTCGTTA	60303405 (Julodis onopordi)

PC001	285	CCAAAGCATGGATGTTGGACAA	90138164 (Spodoptera frugiperda)

PC001	286	CCATTTTTGGTAACACATGATGG	111011915 (Apis mellifera)

PC001	287	CCCAAAGCATGGATGTTGGACAA	50565112 (Homalodisca coagulata)

PC001	288	CCCAAAGCATGGATGTTGGACAAA	103790417 (Heliconius erato)
			101419954 (Plodia interpunctella)

PC001	289	CCCAAAGCATGGATGTTGGACAAATT	73612809 (Aphis gossypii)

PC001	290	CCCAAAGCATGGATGTTGGACAAATTGGG	77329254 (Chironomus tentans)

PC001	291	CCCAAAGCATGGATGTTGGACAAATTGGGGGGTGT	60305420 (Mycetophagus quadripustulatus)

PC001	292	CCCAAAGCATGGATGTTGGACAAATTGGGGGGTGTCTTCGC	84647995 (Myzus persicae)

PC001	293	CGTTACCCTGACCCCAACATCAA	73613065 (Aphis gossypii)

PC001	294	GCAAAATACAAGTTGTGTAAAGTAA	83662334 (Myzus persicae)

PC001	295	GCATGGATGTTGGACAAATTGGG	92969396 (Drosophila grimshawi)

PC001	296	GCATGGATGTTGGACAAATTGGGGG	67885868 (Drosophila pseudoobscura)

PC001	297	GCATGGATGTTGGACAAATTGGGGGGTGT	25956479 (Biphyllus lunatus)

PC001	298	GCATGGATGTTGGACAAATTGGGGGGTGTCT	90814901 (Nasonia vitripennis)

PC001	299	GCTCCCAAAGCATGGATGTTGGA	110260785 (Spodoptera frugiperda)

PC001	300	GCTCCCAAAGCATGGATGTTGGACAA	76551269 (Spodoptera frugiperda)

PC001	301	GCTCCCAAAGCATGGATGTTGGACAAA	56085210 (Bombyx mori)

PC001	302	GCTCCCAAAGCATGGATGTTGGACAAATTGGG	22474232 (Helicoverpa armigera)

PC001	303	GGTCCCAAAGGAATCCCATTTTTGGT	50565112 (Homalodisca coagulata)

PC001	304	GGTGTCTTCGCCCCTCGTCCA	82575022 (Acyrthosiphon pisum)

PC001	305	GTGAAGTCACTAAAATTGTCATGCAAAG	25956820 (Biphyllus lunatus)

PC001	306	TCCACCGGGCCTCACAAGTTGCG	58371410 (Lonomia obliqua)

PC001	307	TCCCAAAGCATGGATGTTGGA	110263957 (Spodoptera frugiperda)

PC001	308	TGCTCCCAAAGCATGGATGTTGGACAA	48927129 (Hydropsyche sp.)

PC001	309	TGGATGTTGGACAAATTGGGGGGTGTCT	90814560 (Nasonia vitripennis)

PC003	310	AAAATTGAAGATTTCTTGGAA	108742519 (Gryllus firmus)
			109978291 (Gryllus pennsylvanicus)
			62083482 (Lysiphlebus testaceipes)
			56150446 (Rhynchosciara americana)

PC003	311	AACAAACGTGAAGTGTGGAGAGT	57963755 (Heliconius melpomene)

PC003	312	AAGTCGCCCTTCGGGGGTGGCCG	77884026 (Aedes aegypti)

PC003	313	ACTTCTCCCTGAAGTCGCCCTTCGG	92992453 (Drosophila mojavensis)

PC003	314	AGATTGTTTGAAGGTAATGCACTTCT	60298816 (Diaphorina citri)

PC003	315	ATCCGTAAAGCTGCTCGTGAA	33373689 (Glossina morsitans)

PC003	316	ATCGACTTCTCCCTGAAGTCGCC	92987113 (Drosophila grimshawi)

PC003	317	ATCGACTTCTCCCTGAAGTCGCCCT	1899548 (Drosophila melanogaster)

PC003	318	ATGAAGCTTGATTATGTTTTGGGTCTGAAAATTG	71539459 (Diaphorina citri)
		AAGATTTCTTGGAAAGA

PC003	319	ATTGAAGATTTCTTGGAAAGA	62240069 (Diabrotica virgifera)

PC003	320	CACATCGACTTCTCCCTGAAGTC	71550961 (Oncometopia nigricans)

PC003	321	CAGAAGCACATCGACTTCTCCCTGAAGTCGCCCTTCGG	68267151 (Drosophila simulans)
			33355000 (Drosophila yakuba)

PC003	322	CAGAAGCACATCGACTTCTCCCTGAAGTCGCCCTTCGGGGG	2152719 (Drosophila melanogaster)

PC003	323	CGACTTCTCCCTGAAGTCGCC	107324644 (Drosophila melanogaster)

PC003	324	CTCCCTGAAGTCGCCCTTCGG	15461311 (Drosophila melanogaster)

PC003	325	CTGGACTCGCAGAAGCACATCGACTTCTCCCTGAA	38624772 (Drosophila melanogaster)

PC003	326	GACTTCTCCCTGAAGTCGCCCTTCGG	92959651 (Drosophila ananassae)
			92981958 (Drosophila mojavensis)
			76552467 (Spodoptera frugiperda)

PC003	327	GCTAAAATCCGTAAAGCTGCTCGTGA	60296953 (Diaprepes abbreviatus)

PC003	328	GCTAAAATCCGTAAAGCTGCTCGTGAACT	77329341 (Chironomus tentans)

PC003	329	GTGCGCAAGCAGGTGGTGAACATCCC	60312414 (Papilio dardanus)

PC003	330	TACACTTTGGCTAAAATCCGTAAAGCTGC	22040140 (Ctenocephalides felis)

PC003	331	TCGCAGAAGCACATCGACTTCTC	18883211 (Anopheles gambiae)

PC003	332	TCGCAGAAGCACATCGACTTCTCCCTGAAGTCGCCCTTCGG	92963738 (Drosophila grimshawi)

PC003	333	TCTCCCTGAAGTCGCCCTTCGG	38047836 (Drosophila yakuba)
			27260897 (Spodoptera frugiperda)

PC003	334	TGAAAATTGAAGATTTCTTGGAA	61646980 (Acyrthosiphon pisum)
			73615225 (Aphis gossypii)
			83661890 (Myzus persicae)
			37804775 (Rhopalosiphum padi)
			30049209 (Toxoptera citricida)

PC003	335	TGAAAATTGAAGATTTCTTGGAAAGA	90813959 (Nasonia vitripennis)

PC003	336	TGGACTCGCAGAAGCACATCGACTTCTCCCT	25959408 (Meladema coriacea)

PC003	337	TGGCTAAAATCCGTAAAGCTGC	76169907 (Diploptera punctata)

PC003	338	TGGGTCTGAAAATTGAAGATTTCTTGGA	34788046 (Callosobruchus maculatus)

PC003	339	TTCTCCCTGAAGTCGCCCTTCGG	107331362 (Drosophila melanogaster)
			110240861 (Spodoptera frugiperda)

PC003	340	TTGGGTCTGAAAATTGAAGATTTCTTGGAAAG	37952462 (Ips pini)

PC003	341	GGGTGCGCAAGCAGGTGGTGAAC	110887729 (Argas monolakensis)

PC005	342	CTCCTCAAAAAGTACAGGGAGGCCAAGAA	63512537 (Ixodes scapularis)

PC005	343	AAAAAGAAGGTGTGGTTGGATCC	33491424 (Trichoplusia ni)

PC005	344	AAAAAGAAGGTGTGGTTGGATCCAAATGAAATCAA	91759273 (Bombyx mori)
			55908261 (Locusta migratoria)

PC005	345	AAAGAAGGTGTGGTTGGATCCAAATGAAATCA	101414616 (Plodia interpunctella)

PC005	346	AACACCAACTCAAGACAAAACAT	25957531 (Cicindela campestris)

PC005	347	AACACCAACTCAAGACAAAACATCCGTAA	25958948 (Curculio glandium)

PC005	348	AACTCAAGACAAAACATCCGTAA	60314333 (Panorpa cf. vulgaris APV-2005)

PC005	349	AAGAACACTGAAGCCAGAAGGAAGGGAAGGCATTGTGG	25958948 (Curculio glandium)

PC005	350	AATGAAATCAACGAAATCGCCAACAC	92979160 (Drosophila grimshawi)
			92232072 (Drosophila willistoni)

PC005	351	ATGGAGTACATCCACAAGAAGAAGGC	15454802 (Drosophila melanogaster)

PC005	352	CAAGATGCTGTCTGACCAGGC	67872905 (Drosophila pseudoobscura)

PC005	353	CGCCTCCTCAAAAAGTACAGGGAGGC	75471260 (Tribolium castaneum)

PC005	354	CGTATCGCCACCAAGAAGCAG	68267374 (Drosophila simulans)

PC005	355	CTGTACATGAAAGCGAAGGGTAA	25957246 (Carabus granulatus)

PC005	356	GAACAAGAGGGTCCTTATGGAG	90977107 (Aedes aegypti)

PC005	357	GAACAAGAGGGTCCTTATGGAGTACATCCA	40544432 (Tribolium castaneum)

PC005	358	GAGCGTATCGCCACCAAGAAGCA	92480972 (Drosophila erecta)
			33354497 (Drosophila yakuba)

PC005	359	GAGTACATCCACAAGAAGAAGGC	15516174 (Drosophila melanogaster)

PC005	360	GATCCAAATGAAATCAACGAAAT	56149737 (Rhynchosciara americana)

PC005	361	GCCAACACCAACTCAAGACAAAACATCCG	103019061 (Tribolium castaneum)

PC005	362	GCCAACACCAACTCAAGACAAAACATCCGTAAGCTCAT	56149737 (Rhynchosciara americana)

PC005	363	GGCAAAAAGAAGGTGTGGTTGGATCCAAATGAAATCA	101417042 (Plodia interpunctella)

PC005	364	GGGTCCTTATGGAGTACATCCACAAGAA	67885759 (Drosophila pseudoobscura)

PC005	365	TGCGATGCGGCAAAAAGAAGGT	56149531 (Rhynchosciara americana)

PC005	366	TGGTTGGATCCAAATGAAATCAACGAAAT	15355452 (Apis mellifera)
			83662749 (Myzus persicae)

PC005	367	TTGGATCCAAATGAAATCAACGAAAT	110985444 (Apis mellifera)
			111158439 (Myzus persicae)

PC010	368	CCGCAGTTCATGTACCATTTG	92952825 (Drosophila ananassae)

PC010	369	CTGATGGAGATGAAGCAGTGCTGCAATTC	58395529 (Anopheles gambiae str. PEST)

PC010	370	GACGTGCTCAGATGGGTGGACAG	56152422 (Rhynchosciara americana)

PC010	371	GCCCGAGCCTGTGTTGTTGGA	92939820 (Drosophila virilis)

PC010	372	GGCACATGCTGATGCGTGAGGAT	83937570 (Lutzomyia longipalpis)

PC010	373	GGGCACATGGTCATGGGCGATTC	3337934 (Drosophila melanogaster)

PC014	374	AAGATCATGGAGTACTACGAGAA	85577611 (Aedes aegypti)

PC014	375	ACGAGAAAAAGGAGAAGCAAG	67838315 (Drosophila pseudoobscura)

PC014	376	ATGGAGTACTACGAGAAAAAGGAGAAGCAAGT	92928915 (Drosophila virilis)

PC014	377	CAAAAACAAATCAAACACATGATGGC	82574001 (Acyrthosiphon pisum)
			111160670 (Myzus persicae)

PC014	378	CTCAAGATCATGGAGTACTACGA	55692554 (Drosophila yakuba)

PC014	379	CTCAAGATCATGGAGTACTACGAGAA	92942301 (Drosophila ananassae)
			92476196 (Drosophila erecta)
			53884266 (Plutella xylostella)

PC014	380	GAACAAGAAGCCAATGAGAAAGC	111160670 (Myzus persicae)

PC014	381	GACTCAAGATCATGGAGTACT	112432414 (Myzus persicae)

PC014	382	GATGTTCAAAAACAAATCAAACACATGATGGC	73618688 (Aphis gossypii)

PC014	383	TACTACGAGAAAAAGGAGAAGC	62239529 (Diabrotica virgifera)

PC014	384	TTCATTGAACAAGAAGCCAATGA	15357365 (Apis mellifera)

PC016	385	ACACGACCGGCGCGCTCGTAAAT	75710699 (Tribolium castaneum)

PC016	386	ACCAGCACGTGCTTCTCGCACTGGTAGGCCAAGAATTCGGC	92048971 (Drosophila willistoni)

PC016	387	AGCACGTGCTTCTCGCACTGGTAGGC	92985459 (Drosophila grimshawi)

PC016	388	ATACGCGACCACGGGTTGATCGG	18868609 (Anopheles gambiae)
			31206154 (Anopheles gambiae str. PEST)

PC016	389	ATCGGTGTACATGTAACCGGGGAAACC	2921501 (Culex pipiens)
			62239897 (Diabrotica virgifera)
			92957249 (Drosophila ananassae)
			92477818 (Drosophila erecta)
			92965644 (Drosophila grimshawi)
			24646342 (Drosophila melanogaster)
			67896654 (Drosophila pseudoobscura)
			75710699 (Tribolium castaneum)

PC016	390	ATCGTTGGCCAAGTTCAAGAACAG	92950254 (Drosophila ananassae)

PC016	391	CACGTGCTTCTCGCACTGGTAGGCCAAGAA	4680479 (Aedes aegypti)

PC016	392	CCAGTCTGGATCATTTCCTCGGG	67884189 (Drosophila pseudoobscura)

PC016	393	CCAGTCTGGATCATTTCCTCGGGATA	92940287 (Drosophila virilis)

PC016	394	CGCTCGATGGTCGGATCGTTGGCCAAGTTCAAGAACA	2921501 (Culex pipiens)

PC016	395	CGCTCGATGGTCGGATCGTTGGCCAAGTTCAAGAACAGACA	92477818 (Drosophila erecta)
		CACGTTCTCCAT	15061308 (Drosophila melanogaster)

PC016	396	CGTGCTTCTCGCACTGGTAGGCCAAGAA	13752998 (Drosophila melanogaster)

PC016	397	CTGGCAGTTTCCATGTTGACACCCATAGC	16898595 (Ctenocephalides felis)

PC016	398	CTTAGCATCAATACCTGATGT	61646107 (Acyrthosiphon pisum)

PC016	399	GACATGTCGGTCAAGATGACCAGCACGTG	9713 (Manduca sexta)

PC016	400	GACATGTCGGTCAAGATGACCAGCACGTGCTTCTCGCACTG	92933153 (Drosophila virilis)

PC016	401	GACATGTCGGTCAAGATGACCAGCACGTGCTTCTCGCACTG	2921501 (Culex pipiens)
		GTA

PC016	402	GAGCCGTTCTCTTCGAAGTCCTG	237458 (Heliothis virescens)

PC016	403	GATGACCAGCACGTGCTTCTC	18883474 (Anopheles gambiae)

PC016	404	GATGACCAGCACGTGCTTCTCGCACTG	92477818 (Drosophila erecta)

PC016	405	GATGACCAGCACGTGCTTCTCGCACTGGTAGGCCAAGAA	15061308 (Drosophila melanogaster)
			67883622 (Drosophila pseudoobscura)

PC016	406	GATGACCAGCACGTGCTTCTCGCACTGGTAGGCCAAGAATTC	31206154 (Anopheles gambiae str. PEST)
		GGC

PC016	407	GATGGGGATCTGCGTGATGGA	101403557 (Plodia interpunctella)

PC016	408	GATGGGGATCTGCGTGATGGAGCCGTTGCGGCCCTCCAC	53883819 (Plutella xylostella)

PC016	409	GGAATAGGATGGGTGATGTCGTCGTTGGGCATAGT	110240379 (Spodoptera frugiperda)

PC016	410	GGAATAGGATGGGTGATGTCGTCGTTGGGCATAGTCA	27372076 (Spodoptera littoralis)

PC016	411	GGATCGTTGGCCAAGTTCAAGAA	91757299 (Bombyx mori)

PC016	412	GGATCGTTGGCCAAGTTCAAGAACA	103020368 (Tribolium castaneum)

PC016	413	GGATCGTTGGCCAAGTTCAAGAACAG	237458 (Heliothis virescens)

PC016	414	GGATGGGTGATGTCGTCGTTGGGCAT	101403557 (Plodia interpunctella)

PC016	415	GGCAGTTTCCATGTTGACACCCATAGC	4680479 (Aedes aegypti)

PC016	416	GGCATAGTCAAGATGGGGATCTG	92924977 (Drosophila virilis)

PC016	417	GTCTGGATCATTTCCTCGGGATA	92966144 (Drosophila grimshawi)

PC016	418	GTGATGATGCGCTCGATGGTCGGATCGTTGGCCAAGTTCAA	15514750 (Drosophila melanogaster)
		GAACAGACACACGTTCTCCAT

PC016	419	GTGTACATGTAACCGGGGAAACC	92924977 (Drosophila virilis)

PC016	420	GTTTCCATGTTGACACCCATAGC	91826756 (Bombyx mori)

PC016	421	TCAATGGGTTTTCCTGATCCATTGAA	49395165 (Drosophila melanogaster)
			99009492 (Leptinotarsa decemlineata)

PC016	422	TCATCCAGCACAGACTTGCCAG	10763875 (Manduca sexta)

PC016	423	TCATCCAGCACAGACTTGCCAGG	9713 (Manduca sexta)

PC016	424	TCCATGTTGACACCCATAGCAGC	92962756 (Drosophila ananassae)

PC016	425	TCCATGTTGACACCCATAGCAGCAAACAC	60295607 (Homalodisca coagulata)

PC016	426	TCGAAGTCCTGCTTGAAGAACCTGGC	101403557 (Plodia interpunctella)

PC016	427	TCGATGGTCGGATCGTTGGCCAAGTTCAAGAACAGACACAC	4680479 (Aedes aegypti)
		GTTCTCCAT

PC016	428	TCGGATCGTTGGCCAAGTTCAAGAACAGACACACG	2793275 (Drosophila melanogaster)
		TTCTCCAT

PC016	429	TCGTTGGCCAAGTTCAAGAACAG	90137502 (Spodoptera frugiperda)

PC016	430	TGGGTGATGTCGTCGTTGGGCAT	53883819 (Plutella xylostella)

PC016	431	TTCTCGCACTGGTAGGCCAAGAA	110240379 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

PC016	432	TTCTCTTCGAAGTCCTGCTTGAAGAACCTGGC	9713 (Manduca sexta)

PC016	433	TTGGCCAAGTTCAAGAACAGACACACGTT	55905051 (Locusta migratoria)

PC016	434	GTTTCCATGTTGACACCCATAGCAGCAAA	84116133 (Dermatophagoides farinae)

TABLE 4-EV

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

EV005	533	AAGCGACGTGAAGAGCGTATCGC	76553206 (Spodoptera frugiperda)

EV005	534	ATTAAAGATGGTCTTATTATTAA	15355452 (Apis mellifera)

EV005	535	CGTAAGCGACGTGAAGAGCGTATCGC	33491424 (Trichoplusia ni)

EV005	536	GGTCGTCATTGTGGATTTGGTAAAAG	60314333 (Panorpa cf. vulgaris APV-2005)

EV005	537	TGCGATGCGGCAAGAAGAAGGT	15048930 (Drosophila melanogaster)

EV005	538	TGCGGCAAGAAGAAGGTTTGG	93002524 (Drosophila mojavensis)
			92930455 (Drosophila virilis)
			92044532 (Drosophila willistoni)

EV005	539	TTGTGGATTTGGTAAAAGGAA	60306723 (Sphaerius sp.)

EV010	540	CAAGTGTTCAATAATTCACCA	83937567 (Lutzomyia longipalpis)

EV010	541	CATTCTATAGGCACATGTTGATG	29558345 (Bombyx mori)

EV010	542	CTGGCGGCCACATGGTCATGGG	92476940 (Drosophila erecta)
			92977931 (Drosophila grimshawi)
			2871327 (Drosophila melanogaster)

EV015	543	AACAGGCCCAATTCCATCGACCC	92947821 (Drosophila ananassae)

EV015	544	AGAGAAAAAATGGACCTCATCGAC	62239128 (Diabrotica virgifera)

EV015	545	CGCCATCCGTCGCTGTTCAAGGCGATCGG	18866954 (Anopheles gambiae)

EV015	546	CTGGCAGTTACCATGGAGAACTTCC	62239128 (Diabrotica virgifera)
		GTTACGCCATG

EV015	547	GTGATCGTGATGGCGGCCACGAA	18887285 (Anopheles gambiae)

EV015	548	GTGATCGTGATGGCGGCCACGAAC	83423460 (Bombyx mori)

EV015	549	TGATGGACGGCATGAAGAAAAG	91086234 (Tribolium castaneum)

EV016	550	AATATGGAAACAGCCAGATTCTT	109193659 (Myzus persicae)

EV016	551	ATGATCCAGACTGGTATTTCTGC	92938857 (Drosophila virilis)

EV016	552	ATTGATGTGATGAATTCCATTGCC	55905051 (Locusta migratoria)

EV016	553	GAAATGATCCAGACTGGTATTTCTGC	50562965 (Homalodisca coagulata)

EV016	554	GAAGAAATGATCCAGACTGGTAT	92969748 (Drosophila mojavensis)

EV016	555	GACTGTGTCTGGTGTGAACGG	2286639 (Drosophila melanogaster)
			92042621 (Drosophila willistoni)

EV016	556	GATATGTTGGGTCGTGTGTTTAA	92969748 (Drosophila mojavensis)

EV016	557	GATCCTACCATTGAAAGAATTAT	99011193 (Leptinotarsa decemlineata)

EV016	558	GTGTCTGAAGATATGTTGGGTCGTGT	76554661 (Spodoptera frugiperda)

EV016	559	GTGTCTGGTGTGAACGGACCG	22474331 (Helicoverpa armigera)

EV016	560	TCTGAAGATATGTTGGGTCGTGT	27372076 (Spodoptera littoralis)

EV016	561	TGGCATATCAATGTGAGAAGCA	60336595 (Homalodisca coagulata)

EV016	562	TTGAACTTGGCCAATGATCCTACCAT	91827863 (Bombyx mori)

TABLE 4-AG

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

AG001	621	AAAACTGGTGAATTCTTCCGTTTGAT	37953169 (Ips pini)

AG001	622	AAAGCATGGATGTTGGACAAA	98994282 (Antheraea mylitta)
			109978109 (Gryllus pennsylvanicus)
			55904580 (Locusta migratoria)

AG001	623	AAAGCATGGATGTTGGACAAATT	31366663 (Toxoptera citricida)

AG001	624	AAAGCATGGATGTTGGACAAATTGGG	60311985 (Papilio dardanus)

AG001	625	AAAGCATGGATGTTGGACAAATTGGGGGGTGT	37951951 (Ips pini)
			109195107 (Myzus persicae)

AG001	626	AAATACAAATTGTGCAAAGTCCG	25958703 (Curculio glandium)

AG001	627	AACTTGTGCATGATCACCGGAG	22039624 (Ctenocephalides felis)

AG001	628	AAGCATGGATGTTGGACAAATTGGGGG	112433559 (Myzus persicae)

AG001	629	AAGCATGGATGTTGGACAAATTGGGGGGTGTGTT	70909486 (Mycetophagus quadripustulatus)

AG001	630	ACTGGTGAATTCTTCCGTTTGAT	77327303 (Chironomus tentans)

AG001	631	ATTTGAAAAAACTGGTGAATTCTTCCGTTTGA	22039624 (Ctenocephalides felis)
		TCTATGATGTTAA

AG001	632	CCAAAGCATGGATGTTGGACAA	90138164 (Spodoptera frugiperda)

AG001	633	CCCAAAGCATGGATGTTGGACAA	48927129 (Hydropsyche sp.)
			76551269 (Spodoptera frugiperda)

AG001	634	CCCAAAGCATGGATGTTGGACAAA	91835558 (Bombyx mori)
			103783745 (Heliconius erato)
			101419954 (Plodia interpunctella)

AG001	635	CCCAAAGCATGGATGTTGGACAAATT	73619372 (Aphis gossypii)

AG001	636	CCCAAAGCATGGATGTTGGACAAATTGGG	77329254 (Chironomus tentans)
			22474232 (Helicoverpa armigera)

AG001	637	CCCAAAGCATGGATGTTGGACAAATTGGGGG	84647382 (Myzus persicae)

AG001	638	CCCAAAGCATGGATGTTGGACAAATTGGGGGGTGT	84647995 (Myzus persicae)

AG001	639	CCCAAAGCATGGATGTTGGACAAATTGGGGGGTGTGTT	60305420 (Mycetophagus quadripustulatus)

AG001	640	CTGGATTCATGGATGTGATCA	27617172 (Anopheles gambiae)

AG001	641	GAATTCTTCCGTTTGATCTATGATGT	50565112 (Homalodisca coagulata)
			71049326 (Oncometopia nigricans)

AG001	642	GCATGGATGTTGGACAAATTGGG	92969396 (Drosophila grimshawi)
			93001617 (Drosophila mojavensis)
			92929731 (Drosophila virilis)

AG001	643	GCATGGATGTTGGACAAATTGGGGG	67885868 (Drosophila pseudoobscura)

AG001	644	GCATGGATGTTGGACAAATTGGGGGGTGT	90814901 (Nasonia vitripennis)

AG001	645	GCATGGATGTTGGACAAATTGGGGGGTGTGTTCGCCCC	25956479 (Biphyllus lunatus)

AG001	646	GCCCCCAAAGCATGGATGTTGGACAA	50565112 (Homalodisca coagulata)

AG001	647	GCTGGATTCATGGATGTGATC	103775903 (Heliconius erato)

AG001	648	GGATCATTCGATATTGTCCACAT	113017118 (Bemisia tabaci)

AG001	649	GGCAACTTGTGCATGATCACCGGAGG	25958703 (Curculio glandium)

AG001	650	TACAAATTGTGCAAAGTCCGCAA	56161193 (Rhynchosciara americana)

AG001	651	TATCCTGCTGGATTCATGGATGT	40934103 (Bombyx mori)

AG001	652	TCACCATTGAAAAAACTGGTGAATTCTTC	62083410 (Lysiphlebus testaceipes)

AG001	653	TGCATGATCACCGGAGGCAGGAA	3478550 (Antheraea yamamai)

AG001	654	TGCATGATCACCGGAGGCAGGAATTTGGG	14627585 (Drosophila melanogaster)
			33355008 (Drosophila yakuba)

AG001	655	TGGATGTTGGACAAATTGGGGGGTGT	90814560 (Nasonia vitripennis)

AG001	656	TGTGCATGATCACCGGAGGCAG	92949859 (Drosophila ananassae)
			92999306 (Drosophila grimshawi)

AG001	657	TGTGCATGATCACCGGAGGCAGGAATTTGGG	67842487 (Drosophila pseudoobscura)

AG005	658	AAGATCGACAGGCATCTGTACCACG	83935651 (Lutzomyia longipalpis)

AG005	659	AAGATCGACAGGCATCTGTACCACGCCCTGTACATGAAGGC	76552995 (Spodoptera frugiperda)

AG005	660	AAGGGTAACGTGTTCAAGAACAA	18932248 (Anopheles gambiae)
			60306606 (Sphaerius sp.)

AG005	661	AAGGGTAACGTGTTCAAGAACAAG	18953735 (Anopheles gambiae)
			25957811 (Cicindela campestris)
			60311920 (Euclidia glyphica)

AG005	662	AAGGGTAACGTGTTCAAGAACAAGAGAGT	25958948 (Curculio glandium)
			90812513 (Nasonia giraulti)

AG005	663	ACAAGAAGAAGGCTGAGAAGGC	60311700 (Euclidia glyphica)

AG005	664	ATCAAGGATGGTTTGATCATTAA	25957811 (Cicindela campestris)

AG005	665	ATGGAATACATCCACAAGAAGAAG	56149737 (Rhynchosciara americana)

AG005	666	CAAAACATCCGTAAATTGATCAAGGATGGT	60314333 (Panorpa cf. vulgaris APV-2005)

AG005	667	CAAAACATCCGTAAATTGATCAAGGATGGTTTGATCAT	25958948 (Curculio glandium)

AG005	668	CAAGGGTAACGTGTTCAAGAA	476608 (Drosophila melanogaster)
			38048300 (Drosophila yakuba)

AG005	669	CAAGGGTAACGTGTTCAAGAACAAG	92946023 (Drosophila ananassae)
			2871633 (Drosophila melanogaster)
			68267374 (Drosophila simulans)
			33354497 (Drosophila yakuba)
			83937096 (Lutzomyia longipalpis)

AG005	670	CATCTGTACCACGCCCTGTACATGAAGGC	101417042 (Plodia interpunctella)

AG005	671	GAAGAAGGCTGAGAAGGCCCG	40874303 (Bombyx mori)

AG005	672	GACAGGCATCTGTACCACGCCCTGTACATGAAGGC	90135865 (Bicyclus anynana)

AG005	673	GAGAAGGCCCGTGCCAAGATGTTG	82572137 (Acyrthosiphon pisum)

AG005	674	GATCCAAATGAAATCAATGAGATTGC	60312128 (Papilio dardanus)

AG005	675	GCTCGTATGCCTCAAAAGGAACTATGG	25957246 (Carabus granulatus)

AG005	676	GGGTAACGTGTTCAAGAACAAG	4447348 (Drosophila melanogaster)

AG005	677	GGTAACGTGTTCAAGAACAAG	18948649 (Anopheles gambiae)

AG005	678	TACATCCACAAGAAGAAGGCTGAGAAG	2871633 (Drosophila melanogaster)

AG005	679	TACCACGCCCTGTACATGAAGGC	10764114 (Manduca sexta)

AG005	680	TCAATGAGATTGCCAACACCAACTC	83935651 (Lutzomyia longipalpis)

AG005	681	TGATCAAGGATGGTTTGATCAT	77642775 (Aedes aegypti)
			27615052 (Anopheles gambiae)
			92982271 (Drosophila grimshawi)
			67896961 (Drosophila pseudoobscura)

AG005	682	TGATCAAGGATGGTTTGATCATTAAGAA	92042883 (Drosophila willistoni)

AG005	683	TGGTTGGATCCAAATGAAATCA	40867709 (Bombyx mori)
			101417042 (Plodia interpunctella)

AG005	684	TGGTTGGATCCAAATGAAATCAA	15355452 (Apis mellifera)
			83662749 (Myzus persicae)

AG005	685	TGGTTGGATCCAAATGAAATCAATGAGAT	63013469 (Bombyx mori)
			55908261 (Locusta migratoria)

AG005	686	TGTACCACGCCCTGTACATGAAGGC	23573622 (Spodoptera frugiperda)

AG005	687	TTGATCAAGGATGGTTTGATCA	113019292 (Bemisia tabaci)

AG005	688	TTGATCAAGGATGGTTTGATCAT	61674956 (Aedes aegypti)
			41576849 (Culicoides sonorensis)

AG005	689	TTGATGGAATACATCCACAAGAAGAAGGC	92225847 (Drosophila willistoni)

AG005	690	AGGATGCGTGTCTTGAGGCGTCT	110887217 (Argas monolakensis)

AG005	691	AAGGCCAAGGGTAACGTGTTCAAGAACAAG	110887217 (Argas monolakensis)

AG010	692	CGTTTGTGTCAAAAGTTTGGAGAATA	78539702 (Glossina morsitans)

AG010	693	GATGTTTTAAGATGGGTCGATCG	110759793 (Apis mellifera)

AG010	694	TTTTACAGGCATATGCTTATGAGGGAAGATTT	55902158 (Locusta migratoria)

AG010	695	TTTTTCGAGGTGGTCAATCAGCATTCGGC	92925934 (Drosophila virilis)

AG014	696	AACATGCTGAACCAAGCCCGT	75466802 (Tribolium castaneum)

AG014	697	AACATGCTGAACCAAGCCCGTCT	87266590 (Choristoneura fumiferana)
			103779114 (Heliconius erato)

AG014	698	AAGATCATGGAATACTATGAGAAGAA	101403826 (Plodia interpunctella)

AG014	699	AAGATCATGGAATACTATGAGAAGAAGGAGAA	81520950 (Lutzomyia longipalpis)

AG014	700	AATGAAAAGGCCGAGGAAATTGATGC	62239529 (Diabrotica virgifera)

AG014	701	ATGGAATACTATGAGAAGAAGGA	16901350 (Ctenocephalides felis)

AG014	702	CAATCCTCCAACATGCTGAACCA	53148472 (Plutella xylostella)

AG014	703	CAGATCAAGCATATGATGGCCTTCAT	53148472 (Plutella xylostella)

AG014	704	GCAGATCAAGCATATGATGGCCTTCAT	87266590 (Choristoneura fumiferana)
			9732 (Manduca sexta)
			90814338 (Nasonia vitripennis)

AG014	705	GCGGAAGAAGAATTTAACATTGAAAAGGG	50558386 (Homalodisca coagulata)
			71552170 (Oncometopia nigricans)

AG016	706	AACGACGACATCACCCATCCTATTC	110248186 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

AG016	707	AACGGTTCCATGGAGAACGTGTG	2921501 (Culex pipiens)
			92950254 (Drosophila ananassae)
			110240379 (Spodoptera frugiperda)

AG016	708	AACGGTTCCATGGAGAACGTGTGTCT	24646342 (Drosophila melanogaster)

AG016	709	AACGGTTCCATGGAGAACGTGTGTCTCTTCTTGAA	91829127 (Bombyx mori)

AG016	710	ATGATCCAGACCGGTATCTCCGC	22474040 (Helicoverpa armigera)

AG016	711	ATGCCGAACGACGACATCACCCATCC	31206154 (Anopheles gambiae str. PEST)

AG016	712	CAATGCGAGAAACACGTGCTGGT	9713 (Manduca sexta)

AG016	713	CCGCACAACGAAATCGCCGCCCAAAT	75469507 (Tribolium castaneum)

AG016	714	CGTTTCTTCAAGCAGGACTTCGA	83937868 (Lutzomyia longipalpis)

AG016	715	CTTGGACATCCAAGGTCAACCCATCAACCCATGGTC	104530890 (Belgica antarctica)

AG016	716	GAAATGATCCAGACCGGTATCTC	2921501 (Culex pipiens)
			92966144 (Drosophila grimshawi)

AG016	717	GAAATGATCCAGACCGGTATCTCCGCCATCGACGTGATGAAC	31206154 (Anopheles gambiae str. PEST)
		TC

AG016	718	GAAGAAATGATCCAGACCGGTAT	75469507 (Tribolium castaneum)

AG016	719	GAAGAAGTACCCGGACGTCGTGG	22038926 (Ctenocephalides felis)

AG016	720	GACATCCAAGGTCAACCCATCAA	16898595 (Ctenocephalides felis)

AG016	721	GCCCGTTTCTTCAAGCAGGACTTCGA	31206154 (Anopheles gambiae str. PEST)

AG016	722	GCCGCCCAAATCTGTAGACAGGC	60295607 (Homalodisca coagulata)

AG016	723	GGATCAGGAAAACCCATTGACAAAGGTCC	49395165 (Drosophila melanogaster)
			99009492 (Leptinotarsa decemlineata)

AG016	724	GGTTACATGTACACCGATTTGGC	91829127 (Bombyx mori)

AG016	725	GGTTACATGTACACCGATTTGGCCACCAT	77750765 (Aedes aegypti)
			9713 (Manduca sexta)
			110248186 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

AG016	726	GGTTACATGTACACCGATTTGGCCACCATTTACGAA	92231646 (Drosophila willistoni)

AG016	727	GTGTCGGAGGATATGTTGGGCCG	92460250 (Drosophila erecta)
			24646342 (Drosophila melanogaster)
			55694673 (Drosophila yakuba)

AG016	728	TACATGTACACCGATTTGGCCACCAT	31206154 (Anopheles gambiae str. PEST)

AG016	729	TTCAACGGATCAGGAAAACCCATTGACAAAGGTCC	99010653 (Leptinotarsa decemlineata)

AG016	730	TTCCCCGGTTACATGTACACCGATTTGGCCAC	2921501 (Culex pipiens)
			75710699 (Tribolium castaneum)

AG016	731	TTCCCCGGTTACATGTACACCGATTTGGCCACCAT	62239897 (Diabrotica virgifera)
			92957249 (Drosophila ananassae)
			92477149 (Drosophila erecta)
			67896654 (Drosophila pseudoobscura)

AG016	732	TTCCCCGGTTACATGTACACCGATTTGGCCACCATTTA	92969578 (Drosophila grimshawi)

AG016	733	TTCCCCGGTTACATGTACACCGATTTGGCCACCATTTACGA	103744758 (Drosophila melanogaster)

AG016	734	TTCGCCATCGTGTTCGCCGCCATGGGTGT	31206154 (Anopheles gambiae str. PEST)

AG016	735	TTCTTCAAGCAGGACTTCGAAGA	9713 (Manduca sexta)

AG016	736	TTCTTGAATTTGGCCAACGATCC	92972277 (Drosophila grimshawi)
			99011193 (Leptinotarsa decemlineata)

AG016	737	TTCTTGAATTTGGCCAACGATCCCACCATCGAG	67839381 (Drosophila pseudoobscura)

AG016	738	GCCGAATTTTTGGCTTATCAATG	84116133 (Dermatophagoides farinae)

TABLE 4-TC

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

TC001	813	AAAGCATGGATGTTGGATAAA	70909480 (Carabus granulatus)
			16898765 (Ctenocephalides felis)
			60298000 (Diaprepes abbreviatus)

TC001	814	AATTTGTGTATGATTACTGGAGG	55904576 (Locusta migratoria)

TC001	815	ACTGGAGGTCGTAACTTGGGGCGTGT	60298000 (Diaprepes abbreviatus)

TC001	816	ATGATTACTGGAGGTCGTAACTTGGGGCGTGT	73619372 (Aphis gossypii)
			37804548 (Rhopalosiphum padi)

TC001	817	ATGCAAAGATTGATTAAAGTTGACGG	70909478 (Biphyllus lunatus)

TC001	818	ATTAAAGTTGACGGAAAAGTT	110763874 (Apis mellifera)

TC001	819	ATTGAGAAAACTGGGGAATTCTTCCG	37952206 (Ips pini)

TC001	820	ATTGTTATGCAAAGATTGATTAAAGTTGACGGAAAAGT	70909486 (Mycetophagus quadripustulatus)

TC001	821	CCAAGAAGCATTTGAAGCGTCT	55904580 (Locusta migratoria)

TC001	822	CCAAGAAGCATTTGAAGCGTCTC	83935971 (Lutzomyia longipalpis)

TC001	823	GCGCCCAAAGCATGGATGTTGGA	103790417 (Heliconius erato)
			101419954 (Plodia interpunctella)

TC001	824	GGCCCCAAGAAGCATTTGAAGCGT	14700642 (Drosophila melanogaster)

TC001	825	TGATTACTGGAGGTCGTAACTTGGGGCGTGT	73612212 (Aphis gossypii)

TC001	826	TGTATGATTACTGGAGGTCGTAACTTGGGGCGTGT	70909478 (Biphyllus lunatus)

TC001	827	TTGATTTATGATGTTAAGGGA	77325485 (Chironomus tentans)

TC001	828	TTGTGTATGATTACTGGAGGTCGTAA	60305816 (Mycetophagus quadripustulatus)

TC002	829	AAAAACAAACGAGCGGCCATCCAGGC	18920284 (Anopheles gambiae)

TC002	830	ATCGACCAAGAGATCCTCACAGCGAAGAAAAACGCGTCGAAA	75717966 (Tribolium castaneum)
		AACAAACGAGCGGCCATCCAGGCC

TC002	831	CTCCAGCAGATCGATGGCACCCT	92475657 (Drosophila erecta)
			13763220 (Drosophila melanogaster)

TC002	832	TCAAGAGGAAGAAACGCTACGAAAAGCAGCTCCAGCAGATC	75717966 (Tribolium castaneum)
		GATGGCACCCTCAGCACCATCGAGATGCAGCGGGAGGCCCT
		CGAGGGGGCCAACACCAACACAGCCGTACTCAAAACGATGA
		AAAACGCAGCGGACGCCCTCAAAAATGCCCACCTCAACATG
		GATGTTGATGAGGT

TC010	833	AACCTCAAGTACCAGGACATGCCCGA	90973566 (Aedes aegypti)

TC010	834	AGCCGATTTTGTACAGTTATA	92944620 (Drosophila ananassae)

TC010	835	ATGGACACATTTTTCCAAATT	33427937 (Glossina morsitans)

TC010	836	ATGGACACATTTTTCCAAATTTTGATTTTCCACGG	56151768 (Rhynchosciara americana)

TC010	837	CAAGTACCAGGACATGCCCGA	18911059 (Anopheles gambiae)

TC010	838	CACATGCTGATGCGGGAGGACCTC	67893321 (Drosophila pseudoobscura)

TC010	839	CCTCAAGTACCAGGACATGCCCGA	67893324 (Drosophila pseudoobscura)

TC010	840	TCAAGTACCAGGACATGCCCGA	67893321 (Drosophila pseudoobscura)

TC010	841	TTCATGTACCATTTGCGCCGCTC	92952825 (Drosophila ananassae)

TC014	842	AAAATTCAGTCGTCAAACATGCTGAA	76169390 (Diploptera punctata)

TC014	843	AACATGCTGAACCAAGCCCGT	87266590 (Choristoneura fumiferana)
			103779114 (Heliconius erato)

TC014	844	CACAGCAACTTGTGCCAGAAAT	92923718 (Drosophila virilis)

TC014	845	GAGAAAGCCGAAGAAATCGATGC	77325830 (Chironomus tentans)

TC014	846	GCCCGCAAACGTCTGGGCGAA	92232132 (Drosophila willistoni)

TC014	847	TAAAAGTGCGTGAAGACCACGT	58371699 (Lonomia obliqua)

TC015	848	ACACTGATGGACGGCATGAAGAA	78531609 (Glossina morsitans)

TC015	849	ATCGGCGGTTGTCGCAAACAACT	6904417 (Bombyx mori)

TC015	850	CCCGATGAGAAGATCCGGATGAA	83922984 (Lutzomyia longipalpis)

TC015	851	CTGCCCCGATGAGAAGATCCG	92948836 (Drosophila ananassae)

TC015	852	AACGAAACCGGTGCTTTCTTCTT	84116975 (Dermatophagoides farinae)

TABLE 4-MP

Target	SEQ
ID	ID NO	Sequence *	Example Gi-number and species

MP001	908	AAAGCATGGATGTTGGACAAA	98994282 (Antheraea mylitta)
			108789768 (Bombyx mari)
			109978109 (Gryllus pennsylvanicus)
			55904580 (Locusta migratoria)

MP001	909	AAAGCATGGATGTTGGACAAAT	77325485 (Chironomus tentans)
			37951951 (lps pini)
			60311985 (Papilio dardanus)
			30031258 (Toxoptera citricida)

MP001	910	AAGAAGCATTTGAAGCGTTTAAACGCACC	3658572 (Manduca sexta)

MP001	911	AAGCATTTGAAGCGTTTAAACGC	103790417 (Heliconius erato)
			22474232 (Helicoverpa armigera)

MP001	912	AAGCATTTGAAGCGTTTAAACGCACC	25957217 (Carabus granulatus)

MP001	913	AAGTCCGTACCGACCCTAATTATCCAGC	46994131 (Acyrthosiphon pisum)

MP001	914	ACGCACCCAAAGCATGGATGTT	46999037 (Acyrthosiphon pisum)

MP001	915	ACTATTAGATACGATATTGCA	46998791 (Acyrthosiphon pisum)

MP001	916	ACTGGACCCAAAGGTGTGCCATTTTTAACTACTCATGATGGC	46997137 (Acyrthosiphon pisum)
		CGTACTAT

MP001	917	AGAAGCATTTGAAGCGTTTAAA	27620566 (Anopheles gambiae)

MP001	918	AGAAGCATTTGAAGCGTTTAAACGCACC	98994282 (Antheraea mylitta)

MP001	919	AGAAGCATTTGAAGCGTTTAAACGCACCCAAAGCATGGATGT	73619191 (Aphis gossypii)
		TGGACAAAT

MP001	920	AGTAAGGGAGTTAAATTGACTA	46998791 (Acyrthosiphon pisum)

MP001	921	ATACAAGTTGTGTAAAGTAAAG	29553519 (Bombyx mori)

MP001	922	ATGGATGTTATATCTATCCAAAAGACCAGTGAGCACTTVAGAT	46998791 (Acyrthosiphon pisum)
		TGATCTATGATGTGAAAGGTCGTTTVCAC

MP001	923	ATTGATCTATGATGTGAAAGGTCGTTTCAC	46999037 (Acyrthosiphon pisum)

MP001	924	CAAAAGACCAGTGAGCACTTTAGATTGAT	30031258 (Toxoptera citricida)

MP001	925	CACAGAATTACTCCTGAAGAAGC	73619191 (Aphis gossypii)

MP001	926	CACAGAATTACTCCTGAAGAAGCAAAATACAAG	46998791 (Acyrthosiphon pisum)
			30031258 (Toxoptera citricida)

MP001	927	CATCCAGGATCTTTTGATATTGTTCACATTAA	31364848 (Toxoptera citricida)

MP001	928	CATCCAGGATCTTTTGATATTGTTCACATTAAGGATGCAAATG	37804548 (Rhopalosiphum padi)
		AACATATTTTTGCTAC

MP001	929	CATCTAAAATTTTGGATCATATCCGTTTTGAAACTGGAAACTT	46998791 (Acyrthosiphon pisum)
		GTGCATGAT

MP001	930	CATTTGAAGCGTTTAAACGCACC	30031258 (Toxoptera citricida)

MP001	931	CATTTGAAGCGTTTAAACGCACCCAAAGCATGGATGTT	46998791 (Acyrthosiphon pisum)

MP001	932	CCAAAGCATGGATGTTGGACAA	90138164 (Spodoptera frugiperda)

MP001	933	CCAAGGAGTAAGGGAGTTAAATTGACTA	73615238 (Aphis gossypii)
			31364848 (Toxoptera citricida)

MP001	934	CCCAAAGCATGGATGTTGGAC	108789768 (Bombyx mori)

MP001	935	CCCAAAGCATGGATGTTGGACAA	50565112 (Homalodisca coagulata)
			48927129 (Hydropsyche sp.)
			76551269 (Spodoptera frugiperda)

MP001	936	CCCAAAGCATGGATGTTGGACAAA	56085210 (Bombyx mori)
			103792451 (Heliconius erato)
			101419954 (Plodia interpunctella)

MP001	937	CCCAAAGCATGGATGTTGGACAAAT	22474095 (Helicoverpa armigera)

MP001	938	CGTCCAAGCACCGGTCCACACAAACT	47537863 (Acyrthosiphon pisum)

MP001	939	CTGGAAACTTGTGCATGATAACTGGAGG	78524585 (Glossina morsitans)

MP001	940	GAAAGACATCCAGGATCTTTTGATATTGTTCACATTAAGGATG	46997137 (Acyrthosiphon pisum)
		CAAATGAACATATTTTTGCTACCCGGATGAACAATGTTTTTAT
		TATTGGAAAAGGTCAAAAGAACTACATTTCTCTACCAAG

MP001	941	GATCATATCCGTTTTGAAACTGGAAACTTGTGCATGAT	73614725 (Aphis gossypii)

MP001	942	GATGCAAATGAACATATTTTTGCTAC	31364848 (Toxoptera citricida)

MP001	943	GCACCCAAAGCATGGATGTTGGA	70909486 (Mycetophagus
			quadripustulatus)

MP001	944	GCACCCAAAGCATGGATGTTGGACAAAT	77329254 (Chironomus tentans)
			60305420 (Mycetophagus
			quadripustulatus)

MP001	945	GGATCTTTTGATATTGTTCACAT	60303405 (Julodis onopordi)

MP001	946	GGATCTTTTGATATTGTTCACATTAAGGATGCAAATGAACATA	73619191 (Aphis gossypii)
		TTTTTGCTAC

MP001	947	GGCCCCAAGAAGCATTTGAAGCGTTTAA	14693528 (Drosophila melanogaster)

MP001	948	GGGCGTGTTGGTATTGTTACCAACAG	31365398 (Toxoptera citricida)

MP001	949	GGGCGTGTTGGTATTGTTACCAACAGGGAAAG	73612212 (Aphis gossypii)
			37804548 (Rhopalosiphum padi)

MP001	950	GGTACAAACTGGACCCAAAGG	60297572 (Diaprepes abbreviatus)

MP001	951	GTTTTTATTATTGGAAAAGGTCAAAAGAACTACATTTCTCT	73619191 (Aphis gossypii)
			31364848 (Toxoptera citricida)

MP001	952	TGAAGTATGCACTTACTGGTGC	73619191 (Aphis gossypii)

MP001	953	TGTAAAGTAAAGAGGGTACAAACTGGACCCAAAGGTGT	73619191 (Aphis gossypii)

MP001	954	TGTGTAAAGTAAAGAGGGTACAAACTGGACCCAAAGGTGT	30031258 (Toxoptera citricida)

MP001	955	TTCTTGCGTAATCGTTTGAAGTATGCACTTACTGGTGCCGAA	46998791 (Acyrthosiphon pisum)
		GTCACCAAGATTGTCATGCAAAGATTAATCAAGGTTGATGGC
		AAAGTCCGTACCGACCCTAATTATCCAGC

MP001	956	TTGGAAAAGGTCAAAAGAACTACATTTCTCT	73615060 (Aphis gossypii)

MP001	957	TTGGATCATATCCGTTTTGAAACTGGAAACTTGTGCATGAT	37804548 (Rhopalosiphum padi)

MP002	958	AAAAAAAATGGTACAACTAATAAACGAGCTGCATTGCAAGC	47537017 (Acyrthosiphon pisum)

MP002	959	AAGAAACGGTACGAACAACAA	15363283 (Apis mellifera)

MP002	960	ACAAGAATTTTTAGAAAAAAAAATTGAACAAGAAGTAGCGATA	47537017 (Acyrthosiphon pisum)
		GC

MP002	961	CAAATTGATGGTACCATGTTAACTATTGAACAACAGCG	47537017 (Acyrthosiphon pisum)

MP002	962	GAAGATGCGATACAAAAGCTTCGATCCAC	47537017 (Acyrthosiphon pisum)

MP002	963	GAGTTTCTTTAGTAAAGTATTCGGTGG	110762684 (Apis mellifera)

MP010	964	AAAAGATGATCCAAATAGTTT	110759793 (Apis mellifera)

MP010	965	AAAATATTATTGATGGACACATTTTTCCATATTTTGATATTCCA	47520567 (Acyrthosiphon pisum)

MP010	966	AATAGTCCTGATGAAACATCATATTATAG	47520567 (Acyrthosiphon pisum)

MP010	967	CAAAAAGATGATCCAAATAGTTTCCGATTGCCAGAAAACTTCA	47520567 (Acyrthosiphon pisum)
		GTTTATATCCACAGTTCATGTATCATTTAAGAAGGTCTCAATTT
		CTACAAGTTTTTAA

MP010	968	CAACATTCCAGTGGCTATAAACGAAT	47520567 (Acyrthosiphon pisum)

MP010	969	CACATGTTGATGCGTGAAGATGTTAC	47520567 (Acyrthosiphon pisum)

MP010	970	CCAATTCTGTATAGCTATAGTTTTAATGGTAGGCCAGAACCTG	47520567 (Acyrthosiphon pisum)
		TACTTTTGGATACCAG

MP010	971	CCATCTCAAACACATAATAATATGTATGCTTATGGAGG	55814942 (Acyrthosiphon pisum)

MP010	972	CTCAAAACTCGATTCCCAATGCCTCGGTATATTGACACAGAA	55814942 (Acyrthosiphon pisum)
		CAAGGTGGTAGTCAGGCAAGATTTTTACTATGCAAAGT

MP010	973	GGTGATGGTGGAGCACCAGTTTTGACAGATGATGTAAGCTTG	55814942 (Acyrthosiphon pisum)
		CA

MP010	974	GTGGCTGCATACAGTTCATTACGCAGTA	28571527 (Drosophila melanogaster)

MP010	975	TAATGGCTCGTATGGTAGTGAACCGTGCTGAAACTGA	47520567 (Acyrthosiphon pisum)

MP010	976	TATAGGCACATGTTGATGCGTGAAGAT	40924332 (Bombyx mori)

MP010	977	TGGGCTGATCGTACGCTTATACGCTTGTGTCA	47520567 (Acyrthosiphon pisum)

MP010	978	TTAGCTAGGAATTGGGCAGACCCTGT	47520567 (Acyrthosiphon pisum)

MP016	979	AAACAAGATTTTGAGGAAAATGG	35508791 (Acyrthosiphon pisum)

MP016	980	AACCTGGTAAATCAGTTCTTGA	35508791 (Acyrthosiphon pisum)

MP016	981	AACGACGACATCACCCATCCTATTC	110240379 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

MP016	982	AATTTAGCTAATGATCCTACTATTGA	15366446 (Apis mellifera)

MP016	983	ACTATGCCTAACGACGACATCACCCATCC	237458 (Heliothis virescens)

MP016	984	ATAGTATTTGCTGCTATGGGTGTTAATATGGAAAC	30124460 (Toxoptera citricida)

MP016	985	CAAATTTGTAGACAAGCTGGTCT	103020368 (Tribolium castaneum)

MP016	986	CATGAAGACAATTTTGCTATAGTATTTGCTGCTATGGGTGTTA	35508791 (Acyrthosiphon pisum)
		ATATGGAAAC

MP016	987	CCGATAGATAAAGGACCTCCTATTTTGGCTGAAGATTATTTGG	35508791 (Acyrthosiphon pisum)
		ATATTGAAGGCCAACCTATTAATCCATA

MP016	988	CCTATTTTGGCTGAAGATTAT	55905051 (Locusta migratoria)

MP016	989	CGTATCATTACACCACGTCTTGCTTTAACTGCTGCTGAATTTT	30124460 (Toxoptera citricida)
		TAGCTTA

MP016	990	CGTCTTGCTTTAACTGCTGCTGAATTTTTAGCTTA	35508791 (Acyrthosiphon pisum)

MP016	991	GAAGAAGTACCTGGGCGTCGTGGTTTCCCTGGTTACATGTAC	30124460 (Toxoptera citricida)
		AC

MP016	992	GAAGGAAGAAATGGTTCTATCACACAAATACCTATTTTAACTA	30124460 (Toxoptera citricida)
		TGCCTAA

MP016	993	GAAGGAAGAAATGGTTCTATCACACAAATACCTATTTTAACTA	73615307 (Aphis gossypii)
		TGCCTAACGA

MP016	994	GATTTAGCTACAATTTATGAACG	30124460 (Toxoptera citricida)

MP016	995	GCCAGATTCTTTAAACAAGATTTTGAGGAAAATGG	30124460 (Toxoptera citricida)

MP016	996	GCTATGGGTGTTAATATGGAAAC	75469507 (Tribolium castaneum)

MP016	997	GCTGCAGGTTTACCACATAATGAGATTGCTGCTCAAATTTG	35508791 (Acyrthosiphon pisum)

MP016	998	GCTGGGCGTGTAGAAGGAAGAAATGGTTCTATCACACAAATA	55813096 (Acyrthosiphon pisum)

TABLE 4-NL

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

NL001	1161	CTGAAGAAGCTAAGTACAAGCT	16566724 (Spodoptera frugiperda)

NL001	1162	TTCTTCCGTTTGATCTATGATGTTAA	16900870 (Ctenocephalides felis)

NL001	1163	CAGCTGAAGAAGCTAAGTACAA	16900870 (Ctenocephalides felis), 56199521 (Culicoides
			sonorensis)

NL001	1164	GAGTTCTTCCGTTTGATCTATGATGTTAA	16900945 (Ctenocephalides felis)

NL001	1165	AAGTACAAGCTGTGCAAAGTGAAG	22474232 (Helicoverpa armigera)

NL001	1166	TTCGACATCGTGCACATCAAGGAC	22474232 (Helicoverpa armigera)

NL001	1167	ATCACAGCTGAAGAAGCTAAGTACAAG	25956820 (Biphyllus lunatus)

NL001	1168	TGTGTATGATCACTGGAGGTCGTAA	25957367 (Carabus granulatus)

NL001	1169	AACGTTTTCATCATCGGCAAG	27613698 (Anopheles gambiae)

NL001	1170	CCAAAATCATGGACTTCATCA	3738704 (Manduca sexta)

NL001	1171	TGATCTATGATGTTAAGGGACG	3738704 (Manduca sexta)

NL001	1172	CATGGATGTTGGACAAATTGGG	37951951 (Ips pini), 56772312 (Drosophila virilis),
			60305420 (Mycetophagus quadripustulatus), 67885868
			(Drosophila pseudoobscura), 77321575 (Chironomus
			tentans), 25956479 (Biphyllus lunatus), 22474232
			(Helicoverpa armigera);

NL001	1173	TTTTGCCACTAGGTTGAACAACGT	37953169 (Ips pini)

NL001	1174	GCAGCGTCTCATCAAGGTTGACGGCAA	48927129 (Hydropsyche sp.)

NL001	1175	AAGGGACGTTTCACCATCCAC	50818668 (Heliconius melpomene)

NL001	1176	AACCTGTGTATGATCACTGGAGG	60293875 (Homalodisca coagulata)

NL001	1177	ACTAACTGTGAAGTGAAGAAAATTGT	60293875 (Homalodisca coagulata)

NL001	1178	TTCTTCCGTTTGATCTATGATGT	60293875 (Homalodisca coagulata), 71047771
			(Oncometopia nigricans)

NL001	1179	TGTATGATCACTGGAGGTCGTAACTTGGG	60297219 (Diaprepes abbreviatus)

NL001	1180	CATGGATGTTGGACAAATTGGGTGG	60311985 (Papilio dardanus)

NL001	1181	GCTGAAGAAGCTAAGTACAAG	68758383 (Acanthoscurria gomesiana)

NL001	1182	GGAGGTCGTAACTTGGGTCGTGT	77327303 (Chironomus tentans)

NL001	1183	TATGATGTTAAGGGACGTTTCACCAT	77327303 (Chironomus tentans)

NL001	1184	CATGGATGTTGGACAAATTGGG	93002561 (Drosophila grimshawi)
			93001617 (Drosophila mojavensis)
			92939328 (Drosophila virilis)
			112433559 (Myzus persicae)

NL001	1185	CTGAAGAAGCTAAGTACAAGCT	110264122 (Spodotera frugiperda)

NL001	1186	GAAGAAGCTAAGTACAAGCTGTG	90820001 (Graphocephala atropunctata)

NL001	1187	TTGCACAGCTTGTACTTAGCTTCTTC	90134075 (Bicyclus anynana)

NL001	1188	AAGTACAAGCTGTGCAAAGTGAAG	112350104 (Helicoverpa armigera)

NL001	1189	ATGATCACTGGAGGTCGTAACTTGGGTCG	113017118 (Bemisia tabaci)

NL001	1190	GGTCGTAACTTGGGTCGTGTGGG	109978109 (Gryllus pennsylvanicus)

NL001	1191	TTCGACATCGTGCACATCAAGGAC	112350104 (Helicoverpa armigera)

NL001	1192	ACATCGTGCACATCAAGGACG	90981811 (Aedes aegypti)

NL003	1193	CAGGAGTTGAAGATCATCGGAGAGTATGG	15457393 (Drosophila melanogaster), 76551770
			(Spodoptera frugiperda)

NL003	1194	CGTAAGGCCGCTCGTGAGCTG	1797555 (Drosophila melanogaster)

NL003	1195	AAGGTAACGCCCTGCTGCGTCG	18863433 (Anopheles gambiae)

NL003	1196	CAGGAGTTGAAGATCATCGGAGAGTA	2459311 (Antheraea yamamai), 49532931 (Plutella
			xylostella)

NL003	1197	GCCAAGTCCATCCATCACGCCCG	33354488 (Drosophila yakuba), 60312414 (Papilio
			dardanus)

NL003	1198	AAGTCCATCCATCACGCCCGT	33528372 (Trichoplusia ni)

NL003	1199	TGTTTGAAGGTAACGCCCTGCT	34788046 (Callosobruchus maculatus)

NL003	1200	CAGGAGTTGAAGATCATCGGAGA	35505798 (Acyrthosiphon pisum), 56772256 (Drosophila
			virilis)

NL003	1201	GTGCGCCTGGACTCGCAGAAGCACAT	38624772 (Drosophila melanogaster)

NL003	1202	GAGTTGAAGATCATCGGAGAGTA	4158332 (Bombyx mori)

NL003	1203	TTGGGTTTAAAAATTGAAGATTTC	56150446 (Rhynchosciara americana)

NL003	1204	TCGCAGAAGCACATTGACTTCTC	56772256 (Drosophila virilis)

NL003	1205	AGAATGAAGCTCGATTACGTC	60306665 (Sphaerius sp.)

NL003	1206	TTTGTGGTGCGCCTGGACTCG	60312414 (Papilio dardanus)

NL003	1207	AGAAGCACATTGACTTCTCGCTGAAGTC	63514675 (Ixodes scapularis)
NL003	1208	TCGCAGAAGCACATTGACTTCTCGCT	70979521 (Anopheles albimanus)

NL003	1209	CTCATCAGACAAAGACATATCAGAGT	71536734 (Diaphorina citri)

NL003	1210	TTGAAGATCATCGGAGAGTATGG	73612958 (Aphis gossypii)

NL003	1211	AAAATTGAAGATTTCCTTGAA	75467497 (Tribolium castaneum)

NL003	1212	CAGAAGCACATTGACTTCTCGCT	77730066 (Aedes aegypti)

NL003	1213	CGTAAGGCCGCTCGTGAGCTG	24661714 (Drosophila melanogaster)

NL003	1214	GCGTGATGGATGGACTTGGCCAA	90813959 (Nasonia vitripennis)

NL003	1215	GCCAAGTCCATCCATCACGCCCG	92467993 (Drosophila erecta)

NL003	1216	GCCAAGTCCATCCATCACGCCCGT	112349903 (Helicoverpa armigera)

NL003	1217	CTCATCAGACAAAGACATATCAGAGT	110671455 (Diaphorina citri)

NL003	1218	CAGGAGTTGAAGATCATCGGAGA	86464397 (Acyrthosiphon pisum)
			92938865 (Drosophila virilis)

NL003	1219	CAGGAGTTGAAGATCATCGGAGAGTATGG	101417830 (Plodia interpunctella)
			110254389 (Spodoptera frugiperda)

NL003	1220	GAGTTGAAGATCATCGGAGAGTA	112984021 (Bombyx mori)

NL003	1221	TCGCAGAAGCACATTGACTTCTC	93002641 (Drosophila mojavensis)
			92938865 (Drosophila virilis)

NL003	1222	TTGAAGATCATCGGAGAGTATGG	111158779 (Myzus persicae)

NL003	1223	CAGAAGCACATTGACTTCTCGCTGAA	92232387 (Drosophila willistoni)

NL003	1224	CTCCGTAACAAGCGTGAGGTGTGG	92232387 (Drosophila willistoni)

NL003	1225	CGTAACAAGCGTGAGGTGTGG	110558371 (Drosophila ananassae)

NL003	1226	GTCAAATACGCCCTGGCCAAGAT	93001117 (Drosophila grimshawi)

NL004	1227	TACGCCCATTTCCCCATCAACTGTGT	14994663 (Spodoptera frugiperda), 53883415 (Plutella
			xylostella)

NL004	1228	TGCTCTCACATCGAAAACATG	22039837 (Ctenocephalides felis)

NL004	1229	AACTTCCTGGGCGAGAAGTACATC	25959088 (Meladema coriacea)

NL004	1230	GCCGTGTACGCCCATTTCCCCATCAACTG	25959088 (Meladema coriacea)

NL004	1231	GTGTACGCCCATTTCCCCATCAACTGTGTGAC	2761563 (Drosophila melanogaster)

NL004	1232	GTGTACGCCCATTTCCCCATCAACTGTGT	33354902 (Drosophila yakuba)

NL004	1233	ATGCGTGCCGTGTACGCCCATTT	33433477 (Glossina morsitans)

NL004	1234	TCAGCTGCCCTCATCCAACAGTC	33491496 (Trichoplusia ni)

NL004	1235	AAGGATATTCGTAAATTCTTGGA	37952094 (Ips pini), 56199511 (Culicoides sonorensis)

NL004	1236	GCCCATTTCCCCATCAACTGTGT	42766318 (Armigeres subalbatus)

NL004	1237	AACTTCCTGGGCGAGAAGTACAT	49547659 (Rhipicephalus appendiculatus)

NL004	1238	AAGAACAAGGATATTCGTAAATTCTTGGA	56152793 (Rhynchosciara americana)

NL004	1239	AACTTCCTGGGCGAGAAGTACATCCG	58079798 (Amblyomma americanum), 49554219 (Boophilus
			microplus)

NL004	1240	CATTTCCCCATCAACTGTGTGAC	60312171 (Papilio dardanus)

NL004	1241	CGTAACTTCCTGGGCGAGAAGTACATCCG	63516417 (Ixodes scapularis)

NL004	1242	AGATCAGCTGCCCTCATCCAACA	71539722 (Diaphorina citri)

NL004	1243	GTGTACGCCCATTTCCCCATCAACTGTGT	24583601 (Drosophila melanogaster)

NL004	1244	TACGCCCATTTCCCCATCAACTGT	113017826 (Bemisia tabaci)

NL004	1245	TACGCCCATTTCCCCATCAACTGTGT	110263092 (Spodoptera frugiperda)

NL004	1246	GCCCATTTCCCCATCAACTGTGT	94468811 (Aedes aegypti)

NL004	1247	ACACAGTTGATGGGGAAATGGGC	90136736 (Bicyclus anynana)

NL004	1248	GCCCATTTCCCCATCAACTGTGT	110671493 (Diaphorina citri)
			110249018 (Spodoptera frugiperda)

NL004	1249	GTCACACAGTTGATGGGGAAATGGGC	87266195 (Choristoneura fumiferana)

NL004	1250	CCATTTCCCCATCAACTGTGT	90981351 (Aedes aegypti)

NL005	1251	AAGGGTAACGTATTCAAGAACAAGCG	1900283 (Drosophila melanogaster)

NL005	1252	AAGGGTAACGTATTCAAGAACAAG	25956594 (Biphyllus lunatus)

NL005	1253	CGTGTATTGATGGAGTTCATTCA	30124405 (Toxoptera citricida), 60294294 (Homalodisca
			coagulata), 71046487 (Oncometopia nigricans), 73612243
			(Aphis gossypii)

NL005	1254	AAAGGTCAAGGAGGCCAAGAAG	67875089 (Drosophila pseudoobscura)

NL005	1255	AAGATGTTGAACGACCAGGCTGAAGC	77324118 (Chironomus tentans)

NL005	1256	ACGTTACCCTTAGCCTTCATGTA	90812513 (Nasonia giraulti)

NL005	1257	AAGGGTAACGTATTCAAGAACAAGCG	45552830 (Drosophila melanogaster)

NL005	1258	CGTGTATTGATGGAGTTCATTCA	112433619 (Myzus persicae)

NL005	1259	AGGTCAAGGAGGCCAAGAAGC	92941126 (Drosophila virilis)

NL005	1260	ACGTTACCCTTAGCCTTCATGTA	90812513 (Nasonia giraulti)

NL005	1261	AAGGGTAACGTATTCAAGAACAAGCG	45552830 (Drosophila melanogaster)

NL006	1262	AGTCCCAGGAACACCTATCAG	21464337 (Drosophila melanogaster)

NL006	1263	ATTATTCCCTTCCCCGATCACAA	24646762 (Drosophila melanogaster)

NL006	1264	CACGCTATCCCATCTCGTATGACAATTGG	24646762 (Drosophila melanogaster)

NL006	1265	TACAAGTTCTGCAAAATTCGAGT	49573116 (Boophilus microplus)

NL006	1266	ATGACAATTGGCCATTTAATTGAATG	50564037 (Homalodisca coagulata)

NL006	1267	ACCTACACGCACTGCGAGATCCA	58384759 (Anopheles gambiae str. PEST)

NL006	1268	GGTGTGGTGGAGTACATTGACAC	58384759 (Anopheles gambiae str. PEST)

NL006	1269	ATTATTCCCTTCCCCGATCACAA	24646762 (Drosophila melanogaster)

NL006	1270	AGTCCCAGGAACACCTATCAG	22026793 (Drosophila melanogaster)

NL006	1271	CACGCTATCCCATCTCGTATGACAATTGG	24646762 (Drosophila melanogaster)

NL006	1272	TCTCGTATGACAATTGGCCATTT	93000469 (Drosophila mojavensis)

NL007	1273	GCAAACAAGTCATGATGTTCAG	15354019 (Apis mellifera)

NL007	1274	GGTATGGGAAAAACTGCTGTATTTGTGTT	15354019 (Apis mellifera)

NL007	1275	GAATGCATTCCTCAAGCTGTA	21068658 (Chironomus tentans)

NL007	1276	TGCAAGAAATTCATGCAAGATCC	21068658 (Chironomus tentans)

NL007	1277	TTCCAAATCAGCAAAGAGTATGA	2890413 (Drosophila melanogaster)

NL007	1278	GATGACGAGGCCAAGCTGACGCT	49536419 (Rhipicephalus appendiculatus)

NL007	1279	TGTGGTTTTGAACATCCATCTGAAGTACAACA	60308907 (Hister sp.)

NL007	1280	GAAAACGAAAAGAACAAAAAG	77642464 (Aedes aegypti)

NL007	1281	GGTATGGGAAAAACTGCTGTATTTGTGTT	110759359 (Apis mellifera)

NL007	1282	GCAAACAAGTCATGATGTTCAG	110759359 (Apis mellifera)

NL007	1283	CTGCAGCAGCACTATGTCAAACTCAA	90137538 (Spodoptera frugiperda)

NL007	1284	GAAAACGAAAAGAACAAAAAG	94468805 (Aedes aegypti)

NL008	1285	TGCCAAGCCTAAAGATTTGGG	60315277 (Dysdera erythrina)

NL008	1286	ATGTTCAAGAAAGTTAATGCTAGAGA	60336214 (Homalodisca coagulata)

NL008	1287	GAGTTGTTGGTGTTCTTTTGGGATG	66522334 (Apis mellifera)

NL008	1288	TTTCAAACAGTTTTGCAGTTCC	75735289 (Tribolium castaneum)

NL008	1289	GAGTTGTTGGTGTTCTTTTGGGATG	110762109 (Apis mellifera)

NL010_1	1290	AAGGACCTGACTGCCAAGCAG	2761430 (Drosophila melanogaster)

NL010_1	1291	GCCAAGCAGATCCAGGACATG	49559867 (Boophilus microplus)

NL010_1	1292	TGCTCGAAGAGCTACGTGTTCCG	49559867 (Boophilus microplus)

NL010_1	1293	AAGAGCTACGTGTTCCGTGGC	92043082 (Drosophila willistoni)

NL010_1	1294	AAGGACCTGACTGCCAAGCAG	92481328 (Drosophila erecta)
			28571527 (Drosophila melanogaster)

NL010_2	1295	ATGGACACATTTTTCCAAATTCTCAT	33427937 (Glossina morsitans)

NL010_2	1296	ACCAGCAGTATTCAACCCGACA	47520567 (Acyrthosiphon pisum)

NL010_2	1297	TATTGATGGACACATTTTTCCA	47520567 (Acyrthosiphon pisum)

NL010_2	1298	TTCAACAACAGTCCTGATGAAAC	55891325 (Locusta migratoria)

NL010_2	1299	ATGGACACATTTTTCCAAATT	56151768 (Rhynchosciara americana), 75736992 (Tribolium
			castaneum)

NL010_2	1300	CCGCAGTTCATGTACCATCTGCG	6932015 (Anopheles gambiae), 29558345 (Bombyx mori)

NL010_2	1301	ATGGACACATTTTTCCAAATT	91086194 (Tribolium castaneum)

NL011	1302	AAGAAGTATGTTGCCACCCTTGG	21640529 (Amblyomma variegatum)

NL011	1303	GACATCAAGGACAGGAAAGTCAAGGCCAAGAGC	25959135 (Meladema coriacea)
		ATAGT

NL011	1304	CAACTACAACTTCGAGAAGCCGTTCCTGTGG	25959135 (Meladema coriacea), 77646995 (Aedes aegypti)

NL011	1305	TACAAGAACGTTCCCAACTGGCA	3114090 (Drosophila melanogaster)

NL011	1306	TGCGAAAACATTCCCATTGTACT	37951963 (Ips pini)

NL011	1307	AGGAAGAAGAACCTTCAGTACTACGA	40544671 (Tribolium castaneum)

NL011	1308	AGCAACTACAACTTCGAGAAGCC	49565237 (Boophilus microplus), 49538692 (Rhipicephalus
			appendiculatus)

NL011	1309	AACAAAGTAGACATCAAGGACAGGAAAGTCAA	76552920 (Spodoptera frugiperda)

NL011	1310	CCCAACTGGCACAGAGATTTAGTG	78230577 (Heliconius erato/himera mixed EST library)

NL011	1311	GATGGTGGTACCGGCAAAACTAC	78538667 (Glossina morsitans)

NL011	1312	TACAAGAACGTTCCCAACTGGCAC	84267747 (Aedes aegypti)

NL011	1313	AACAAAGTAGACATCAAGGACAGGAAAGTCAA	110263840 (Spodoptera frugiperda)

NL011	1314	TTGACTTTCCTGTCCTTGATGTC	90136305 (Bicyclus anynana)

NL011	1315	GACATCAAGGACAGGAAAGTCAAGGC	90813103 (Nasonia vitripennis)

NL011	1316	AGGAAGAAGAACCTTCAGTACTACGA	91091115 (Tribolium castaneum)

NL011	1317	GATGTCGTAGTACTGAAGGTTCTT	90136305 (Bicyclus anynana)

NL011	1318	CAACTACAACTTCGAGAAGCCGTTCCTGTGG	90977910 (Aedes aegypti)

NL011	1319	CCAACCTGGAGTTCGTCGCCATGCC	92465523 (Drosophila erecta)

NL011	1320	GAATTTGAAAAGAAGTATGTTGC	113015058 (Bemisia tabaci)

NL011	1321	CTTCAGTACTACGACATCAGTGCGAA	110086408 (Amblyomma cajennense)

NL011	1322	AGCAACTACAACTTCGAGAAGCC	110086408 (Amblyomma cajennense)

NL011	1323	AAGCTGATCGGTGACCCCAACCTGGAGTT	110086408 (Amblyomma cajennense)

NL012	1324	CACAGTTTGAACAGCAAGCTGG	29552409 (Bombyx mori)

NL012	1325	GCAGCAGACGCAGGCACAGGTAGA	77823921 (Aedes aegypti)

NL012	1326	CACAGTTTGAACAGCAAGCTGG	94435913 (Bombyx mori)

NL013	1327	CAAGCGAAGATGTTGGACATGCT	15536506 (Drosophila melanogaster)

NL013	1328	ATGGTGGTGGGCTGGTACCACTCGCACCC	49547019 (Rhipicephalus appendiculatus)

NL013	1329	GTGGTGGGCTGGTACCACTCGCACCC	58079586 (Amblyomma americanum)

NL013	1330	GTGGGCTGGTACCACTCGCACCC	82848521 (Boophilus microplus)

NL013	1331	AAGATGTTGGACATGCTAAAGCAGACAGG	92229701 (Drosophila willistoni)

NL013	1332	TGTCGGGTGTCGACATCAACAC	92962655 (Drosophila ananassae)

NL013	1333	GTTCCCATGGAAGTTATGGGC	112433067 (Myzus persicae)

NL013	1334	GTGGGCTGGTACCACTCGCACCC	110085175 (Amblyomma cajennense)

NL014	1335	GAGATCGATGCCAAGGCCGAGGA	1033187 (Drosophila melanogaster)

NL014	1336	GAATTCAACATTGAAAAGGGA	16900951 (Ctenocephalides felis)

NL014	1337	GAAGAATTCAACATTGAAAAGGG	47518467 (Acyrthosiphon pisum)

NL014	1338	GAAGCCAATGAGAAAGCCGAAGA	47518467 (Acyrthosiphon pisum)

NL014	1339	TCGTCAAACATGCTGAACCAAGC	61954844 (Tribolium castaneum)

NL014	1340	TTTCATTGAGCAAGAAGCCAATGA	62239529 (Diabrotica virgifera), 76169390 (Diploptera
			punctata), 61954844 (Tribolium castaneum), 16900951
			(Ctenocephalides felis)

NL014	1341	CAAGAAGCCAATGAGAAAGCCGA	111160670 (Myzus persicae)

NL014	1342	TTTCATTGAGCAAGAAGCCAATGA	91092061 (Tribolium castaneum)

NL014	1343	AGAAGCCAATGAGAAAGCCGA	112432414 (Myzus persicae)

NL014	1344	TCGTCAAACATGCTGAACCAAGC	91092061 (Tribolium castaneum)

NL014	1345	GCCAATGAGAAAGCCGAAGAGATCGATGCCAA	93001435 (Drosophila grimshawi)

NL014	1346	AAAGCCGAAGAGATCGATGCCAA	92936169 (Drosophila virilis)

NL014	1347	GAGATCGATGCCAAGGCCGAGGA	24644299 (Drosophila melanogaster)

NL014	1348	GAAGAATTCAACATTGAAAAGGG	86463006 (Acyrthosiphon pisum)
			111160670 (Myzus persicae)

NL014	1349	GAAGAATTCAACATTGAAAAGGGAAGGCT	90819999 (Graphocephala atropunctata)

NL014	1350	AAGAATTCAACATTGAAAAGGG	111158385 (Myzus persicae)

NL015	1351	GAGGTGCTGCGCATCCACACCAA	18887285 (Anopheles gambiae)

NL015	1352	ATCCATGTGCTGCCCATTGATGA	21641659 (Amblyomma variegatum)

NL015	1353	CATGTGCTGCCCATTGATGAT	22039735 (Ctenocephalides felis)

NL015	1354	CTGCGCATCCACACCAAGAACATGAAGTTGG	22474136 (Helicoverpa armigera)

NL015	1355	TTCTTCTTCCTCATCAACGGACC	49552586 (Rhipicephalus appendiculatus)

NL015	1356	GAGATGGTGGAGTTGCCGCTG	58371722 (Lonomia obliqua)

NL015	1357	CAGATCAAAGAGATGGTGGAG	92947821 (Drosophila ananassae)

NL015	1358	ATCAACGGACCCGAGATTATG	92947821 (Drosophila ananassae)

NL015	1359	ATGAAGATGATGGCCGGTGCGTT	92470977 (Drosophila erecta)

NL015	1360	CCGGCCATCATCTTCATCGATGAG	92480997 (Drosophila erecta)

NL015	1361	ATCATCTTCATCGATGAGCTGGACGC	99007898 (Leptinotarsa decemlineata)

NL015	1362	CAGCTGCTGACGCTGATGGACGG	92941440 (Drosophila virilis)

NL015	1363	ATCGACATTGGCATTCCCGATGCCACCGG	92947821 (Drosophila ananassae)

NL016	1364	TCTATGGAGAACGTGTGCCTGTTCTTGAAC	27372076 (Spodoptera littoralis)

NL016	1365	TACCAGTGCGAGAAGCACGTGCT	2921501 (Culex pipiens)

NL016	1366	ATGGAGAACGTGTGCCTGTTCTTGAACCTGGC	31206154 (Anopheles gambiae str. PEST)

NL016	1367	CGTGGCCAGAAAATCCCCATCTT	3945243 (Drosophila melanogaster)

NL016	1368	TGGCCTACCAGTGCGAGAAGCACGTG	4680479 (Aedes aegypti)

NL016	1369	TGGCCACCATCTACGAGCGCGCCGG	53883819 (Plutella xylostella)

NL016	1370	ATGGAGAACGTGTGCCTGTTCTTGAA	67883622 (Drosophila pseudoobscura)

NL016	1371	CCCGAGGAAATGATCCAGACTGG	67883622 (Drosophila pseudoobscura)

NL016	1372	TGGCCTACCAGTGCGAGAAGCACGTGCT	67883622 (Drosophila pseudoobscura), 31206154
			(Anopheles gambiae str. PEST)

NL016	1373	GAGGAGGTGCCCGGCCGTCGTGGTTTCCCCGG	67896654 (Drosophila pseudoobscura)
		TTACATGTACACCGAT

NL016	1374	GAGGGTCGCAACGGCTCCATCAC	67896654 (Drosophila pseudoobscura)

NL016	1375	GAGGTGCCCGGCCGTCGTGGTTTCCCCGGTTAC	75710699 (Tribolium castaneum)
		ATGTACACCGAT

NL016	1376	ATGGAGAACGTGTGCCTGTTCTTGAAC	76554661 (Spodoptera frugiperda)

NL016	1377	TGGCCTACCAGTGCGAGAAGCACGTGCTCGTCA	9992660 (Drosophila melanogaster)
		TCCT

NL016	1378	CGTCGTGGTTTCCCCGGTTACATGTACACCGAT	9992660 (Drosophila melanogaster), 2921501 (Culex
			pipiens), 62239897 (Diabrotica virgifera)

NL016	1379	TGGTCGCGTATCTATCCCGAGGAAATGATCCAG	92999374 (Drosophila grimshawi)
		AC

NL016	1380	TGGTCGCGTATCTATCCCGAGGAAATGATCCAG	92940538 (Drosophila virilis)
		ACTGG

NL016	1381	TCTATGGAGAACGTGTGCCTGTTCTTGAAC	92938622 (Drosophila virilis)

NL016	1382	ATGGAGAACGTGTGCCTGTTCTTGAAC	92950254 (Drosophila ananassae)
			90137502 (Spodoptera frugiperda)

NL016	1383	AACGTGTGCCTGTTCTTGAAC	92946927 (Drosophila ananassae)

NL016	1384	TGGCCTACCAGTGCGAGAAGCACGTGCT	24646342 (Drosophila melanogaster)
			92231646 (Drosophila willistoni)

NL016	1385	TGGCCTACCAGTGCGAGAAGCACGTGCTCGTCA	107256717 (Drosophila melanogaster)
		TCCT

NL016	1386	GCCTACCAGTGCGAGAAGCACGTGCT	92985459 (Drosphila grimshawi)

NL016	1387	GAGGAGGTGCCCGGCCGTCGTGGTTTCCCCGG	92938622 (Drosophila virilis)
		TTACATGTACAC

NL016	1388	GAGGAGGTGCCCGGCCGTCGTGGTTTCCCCGG	92477818 (Drosophila erecta)
		TTACATGTACACCGAT

NL016	1389	GAGGTGCCCGGCCGTCGTGGTTTCCCCGGTTAC	91090030 (Tribolium castaneum)
		ATGTACACCGAT

NL016	1390	CGTCGTGGTTTCCCCGGTTACAT	104530890 (Belgica antarctica)

NL016	1391	CGTCGTGGTTTCCCCGGTTACATGTACACCGAT	92981037 (Drosophila grimshawi)
			24646342 (Drosophila melanogaster)

NL016	1392	CGTGGTTTCCCCGGTTACATGTACACCGAT	92957249 (Drosophila ananassae)

NL016	1393	ATCGGTGTACATGTAACCGGGGAAACCA	103744758 (Drosophila melanogaster)

NL016	1394	CGTCCGGCGCGCTCGTAGATGGT	91829127 (Bombyx mori)

NL016	1395	GAGGGTCGCAACGGCTCCATCAC	92957249 (Drosophila ananassae)

NL018	1396	CGGACGTGGCCTGGTTCATCA	92479742 (Drosophila erecta)

NL019	1397	GTGGTGTACGACTGCACCGACCAGGAGTCGTTC	84343006 (Aedes aegypti)
		AACAAC

NL019	1398	GAAAGTTACATCAGTACCATTGGTGT	113018639 (Bemisia tabaci)

NL019	1399	CACCGACCAGGAGTCGTTCAACAAC	85857059 (Aedes aegypti)

NL019	1400	AGTACCATTGGTGTAGATTTTAAAAT	91087112 (Tribolium castaneum)

NL019	1401	ATTGGTGTAGATTTTAAAATTAG	78542465 (Glossina morsitans)

NL019	1402	GGTGTAGATTTTAAAATTAGAAC	92232411 (Drosophila willistoni)

NL019	1403	GGTGTAGATTTTAAAATTAGAACAAT	90986845 (Aedes aegypti)

NL019	1404	GTTCTAATTTTAAAATCTACAC	92043152 (Drosophila willistoni)

NL019	1405	TGGGACACGGCCGGCCAGGAG	91091115 (Tribolium castaneum)

NL019	1406	TGGGACACGGCCGGCCAGGAGCG	90982219 (Aedes aegypti)

NL019	1407	TGGGACACGGCCGGCCAGGAGCGGT	94433465 (Bombyx mori)

NL019	1408	GACCAGCTGGGCATTCCGTTCCT	10708384 (Amblyomma amenicanum)

NL019	1409	ATTGGTGTAGATTTTAAAATT	18864897 (Anopheles gambiae)

NL019	1410	TGGGACACGGCCGGCCAGGAGCGGTT	18888926 (Anopheles gambiae)

NL019	1411	CAGGAGCGGTTCCGCACGATCAC	21640713 (Amblyomma variegatum)

NL019	1412	ATTGGTGTAGATTTTAAAATTAGAAC	22039832 (Ctenocephalides felis)

NL019	1413	ATTGGTGTAGATTTTAAAATTAG	33378174 (Glossina morsitans)

NL019	1414	TGGGACACGGCCGGCCAGGAG	3738872 (Manduca sexta), 25959135 (Meladema coriacea),
			40542849 (Tribolium castaneum), 67840088 (Drosophila
			pseudoobscura)

NL019	1415	TGGGACACGGCCGGCCAGGAGCGGT	4161805 (Bombyx mori)

NL019	1416	GATGACACATACACAGAAAGTTACATCAGTAC	50562545 (Homalodisca coagulata), 71047909
			(Oncometopia nigricans)

NL019	1417	ACGGCCGGCCAGGAGCGGTTCCG	58378591 (Anopheles gambiae str. PEST)

NL019	1418	AGTACCATTGGTGTAGATTTTAAAAT	61954135 (Tribolium castaneum)

NL019	1419	TAAAGCTTCAGATTTGGGACAC	68758530 (Acanthoscurnia gomesiana)

NL019	1420	ATTTGGGACACGGCCGGCCAGGA	77667315 (Aedes aegypti)

NL019	1421	GTGGTGTACGACTGCACCGACCAGGAGTCGTTC	77705629 (Aedes aegypti)
		AACAAC

NL019	1422	GGTGTAGATTTTAAAATTAGAACAAT	77890715 (Aedes aegypti)

NL019	1423	TGGGACACGGCCGGCCAGGAGCG	82851662 (Boophilus microplus), 49536894 (Rhipicephalus
			appendiculatus)

NL022	1424	TCTTCCTCACCGGTCAGGAGGAGAT	6928515 (Anopheles gambiae)

NL022	1425	AAATTCTCCGAGTTTTTCGACGATGC	91082872 (Tribolium castaneum)

NL022	1426	TTCCTCACCGGTCAGGAGGAGAT	90976120 (Aedes aegypti)

NL022	1427	TAGTATTGGCCACAAATATTGCAGA	92042565 (Drosophila willistoni)

NL023	1428	TATTTGAACATATGGGTGCCGCA	20384699 (Plutella xylostella)

NL023	1429	GAGGGAGAGGAAATGTGGAATCC	22085301 (Helicoverpa armigera)

NL023	1430	CCGAAGATTGTCTGTATTTGAA	27531022 (Apis meilifera)

NL023	1431	GATTCCGTTTGCGAAACCTCC	57929927 (Anopheles gambiae str. PEST)

NL023	1432	GGTGCGTTCGGCTTCCTCTACCT	58380563 (Anopheles gambiae str. PEST)

NL023	1433	CAATTCAATGCTAGGGAAAGG	110759012 (Apis mellifera)

NL023	1434	GAGGGAGAGGAAATGTGGAATCC	55793188 (Helicoverpa assulta)

NL023	1435	CCGAAGATTGTCTGTATTTGAA	58585075 (Apis mellifera)

NL023	1436	GACGTCATCGTCGCCTCCATGCA	91077117 (Tribolium castaneum)

NL027	1437	GGAGACCCTGGAGCTGGTGCG	49543279 (Rhipicephalus appendiculatus)

TABLE 4-CS

Target
ID	SEQ ID NO	Sequence *	Example Gi-number and species

CS001	1730	AAAGCATGGATGTTGGACAAA	73619372 (Aphis gossypii); 77325485 (Chironomus
			tentans);
			22474232 (Helicoverpa armigera); 37951951
			(Ips pini); 60305420 (Mycetophagus
			quadripustulatus); 84647995
			(Myzus persicae)

CS001	1731	AAAGCATGGATGTTGGACAAACT	40877657 (Bombyx mori); 103783745
			(Heliconius erato);
			55904580 (Locusta migratoria); 101413238 (Plodia
			interpunctella)

CS001	1732	AACCGGCTCAAGTACGCGCTCAC	22474232 (Helicoverpa armigera)

CS001	1733	AACCGGCTCAAGTACGCGCTCACCGG	90134075 (Bicyclus anynana)

CS001	1734	AAGATCATGGACTTCATCAAGTT	90134075 (Bicyclus anynana)

CS001	1735	ACCAGATTGAACAACGTGTTCAT	71536878 (Diaphorina citri)
			3658573 (Manduca sexta)

CS001	1736	ATCATGGACTTCATCAAGTTTGAATC	103783745 (Heliconius erato)

CS001	1737	CAAGATCATGGACTTCATCAAGTT	3478550 (Antheraea yamamai)

CS001	1738	CCCCACAAGTTGCGCGAGTGC	63011732 (Bombyx mori)

CS001	1739	CCCGCTGGATTTATGGATGTTGT	101403940 (Plodia interpunctella)

CS001	1740	CCTCCAAGATCATGGACTTCATCAAGTT	22474232 (Helicoverpa armigera)

CS001	1741	CCTGCCGCTGGTGATCTTCCT	27597800 (Anopheles gambiae)

CS001	1742	CGACGGGCCCCAAGAACGTGCC	22474232 (Helicoverpa armigera)

CS001	1743	CTCATCAAGGTCAACGACTCC	103783745 (Heliconius erato)
			112350001 (Helicoverpa armigera)
			101418268 (Plodia interpunctella)

CS001	1744	CTCATCAAGGTCAACGACTCCATCCAGCTCGAC	3738704 (Manduca sexta)
		AT

CS001	1745	CTCATCAAGGTCAACGACTCCATCCAGCTCGAC	53884106 (Plutella xylostella)
		ATCGCCACCT

CS001	1746	CTGCCGCTGGTGATCTTCCTC	27603050 (Anopheles gambiae)

CS001	1747	GACCCCACATATCCCGCTGGATT	103783745 (Heliconius erato)

CS001	1748	GCAGCGACTTATCAAAGTTGA	109978109 (Gryllus pennsylvanicus)

CS001	1749	GCATGGATGTTGGACAAACTGGG	67899746 (Drosophila pseudoobscura)

CS001	1750	GCCACCTCCAAGATCATGGACTTCAT	110259010 (Spodoptera frugiperda)

CS001	1751	GCGCGTGGCGACGGGCCCCAAGAACGTGCC	53884106 (Plutella xylostella)

CS001	1752	GCTGGATTTATGGATGTTGTTT	29553519 (Bombyx mori)

CS001	1753	GGCTCAAGTACGCGCTCACCGG	5498893 (Antheraea yamamai)

CS001	1754	GTGGGCACCATCGTGTCCCGCGAG	3953837 (Bombyx mandarina)
			53884106 (Plutella xylostella)
CS001	1755	GTGGGCACCATCGTGTCCCGCGAGCG	3478550 (Antheraea yamamai)

CS001	1756	GTGGGCACCATCGTGTCCCGCGAGCGACATCC	22474232 (Helicoverpa armigera)
		CGG

CS001	1757	TAAAGCATGGATGTTGGACAA	58371410 (Lonomia obliqua)

CS001	1758	TAAAGCATGGATGTTGGACAAA	60311985 (Papilio dardanus)
			31366663 (Toxoptera citricida)

CS001	1759	TAAAGCATGGATGTTGGACAAACT	109978109 (Gryllus pennsylvanicus)

CS001	1760	TAAAGCATGGATGTTGGACAAACTGGG	98994282 (Antheraea mylitta)

CS001	1761	TACAAGCTGTGCAAGGTGCGGCGCGTGGCGAC	98993531 (Antheraea mylitta)
		GGGCCC

CS001	1762	TACAAGCTGTGCAAGGTGCGGCGCGTGGCGAC	5498893 (Antheraea yamamai)
		GGGCCCCAA

CS001	1763	TACCCCGACCCACTCATCAAGGT	90134075 (Bicyclus anynana)

CS001	1764	TGAACAACGTGTTCATAATCGG	98993531 (Antheraea mylitta)

CS001	1765	TGCGCGAGTGCCTGCCGCTGGT	22474232 (Helicoverpa armigera)

CS001	1766	TGTATGATCACGGGAGGCCGTAACTTGGG	60311445 (Euclidia glyphica)

CS001	1767	TGTATGATCACGGGAGGCCGTAACTTGGGGCG	3953837 (Bombyx mandarina)

CS001	1768	TGTATGATCACGGGAGGCCGTAACTTGGGGCG	91826697 (Bombyx mori)
		CGTGGGCACCATCGTGTCCCGCGAG

CS001	1769	TGTGCAAGGTGCGGCGCGTGGCGACGGGCCC	3478550 (Antheraea yamamai)
		CAAG

CS001	1770	TTGAACAACGTGTTCATAATCGGCAAGGGCACG	3953837 (Bombyx mandarina)
		AA	40915191 (Bombyx mori)

CS002	1771	ATTGAGGCCCAAAGGGAAGCGCTAGAAGG	91849872 (Bombyx mori)

CS002	1772	CACGATCTGATGGATGACATTG	33498783 (Anopheles gambiae)

CS002	1773	GAGTTTCTTTAGTAAAGTATTCGGTGG	110762684 (Apis mellifera)

CS002	1774	TATGAAAAGCAGCTTACCCAGAT	49552807 (Rhipicephalus appendiculatus)

CS003	1175	AGGCACATCCGTGTCCGCAAGCA	10707186 (Amblyomma americanum)

CS003	1776	AAGATTGAGGACTTCTTGGAA	60295192 (Homalodisca coagulata)

CS003	1777	AAGCACATTGACTTCTCGCTGAA	92219983 (Drosophila willistoni)

CS003	1778	ATCAGACAGAGGCACATCCGTGT	27260897 (Spodoptera frugiperda)

CS003	1779	ATCCGTAAGGCTGCCCGTGAG	101413529 (Plodia interpunctella)

CS003	1780	ATCCGTAAGGCTGCCCGTGAGCTG	92042852 (Drosophila willistoni)

CS003	1781	ATCCGTAAGGCTGCCCGTGAGCTGCT	92959651 (Drosophila ananassae)
			112349903 (Helicoverpa armigera)

CS003	1782	ATCCGTAAGGCTGCCCGTGAGCTGCTCAC	90138123 (Spodoptera frugiperda)

CS003	1783	CACATCCGTGTCCGCAAGCAAG	60306665 (Sphaerius sp.)

CS003	1784	CACATCCGTGTCCGCAAGCAAGT	77329341 (Chironomus tentans)

CS003	1785	CACATCCGTGTCCGCAAGCAAGTTG	60306676 (Sphaerius sp.)

CS003	1786	CGCAACAAGCGTGAGGTGTGG	92473214 (Drosophila erecta)
			67888665 (Drosophila pseudoobscura)

CS003	1787	CGTGTCCGCAAGCAAGTTGTGAACATCCC	90134575 (Bicyclus anynana)
			29553137 (Bombyx mori)

CS003	1788	CTCGCTGAAGTCTCCGTTCGGCGGCGGCCG	3986375 (Antheraea yamamai)

CS003	1789	CTCGGTCTGAAGATTGAGGACTT	112349903 (Helicoverpa armigera)
			49532931 (Plutella xylostella)

CS003	1790	CTGGACTCTGGCAAGCACATTGACTTCTC	29553137 (Bombyx mori)
			58371398 (Lonomia obliqua)

CS003	1791	GACTTCTCGCTGAAGTCTCCGTTCGGCGGCGG	60312414 (Papilio dardanus)

CS003	1792	GACTTCTCGCTGAAGTCTCCGTTCGGCGGCGG	49532931 (Plutella xylostella)
		CCG

CS003	1793	GAGGAGAAAGACCCTAAGAGGTTATTCGAAGG	37952462 (Ips pini)
		TAA

CS003	1794	GATCCGTAAGGCTGCCCGTGA	67568544 (Anoplophora glabripennis)

CS003	1795	GATCCGTAAGGCTGCCCGTGAGCTGCT	67843629 (Drosophila pseudoobscura)
			56772258 (Drosophila virilis)

CS003	1796	GATTATGTACTCGGTCTGAAGATTGAGGACTT	101413529 (Plodia interpunctella)

CS003	1797	GGTCTGAAGATTGAGGACTTCTTGGA	2699490 (Drosophila melanogaster)

CS003	1798	GTGTGGAGGGTGAAGTACACGCT	60312414 (Papilio dardanus)

CS003	1799	GTGTTCAAGGCTGGTCTAGCTAAGTC	78230982 (Heliconius erato/himera mixed EST library)

CS003	1800	GTGTTGGATGAGAAGCAGATGAAGCTCGATTAT	112349903 (Helicoverpa armigera)
		GT

CS003	1801	TGAAGATTGAGGACTTCTTGGA	3986375 (Antheraea yamamai)

CS003	1802	TGGACTCTGGCAAGCACATTGACTTCTC	78230982 (Heliconius erato/himera mixed EST library)

CS003	1803	TGGATGAGAAGCAGATGAAGCT	60312414 (Papilio dardanus)

CS003	1804	TGGTCTCCGCAACAAGCGTGAGGT	76552467 (Spodoptera frugiperda)

CS003	1805	TGGTCTCCGCAACAAGCGTGAGGTGTGG	33528372 (Trichoplusia ni)

CS006	1806	CGTATGACAATTGGTCACTTGATTGA	91831926 (Bombyx mori)

CS006	1807	GAAGATATGCCTTTCACTTGTGAAGG	55801622 (Acyrthosiphon pisum)

CS006	1808	GGAAAAACTATAACTTTGCCAGAAAA	40926289 (Bombyx mori)

CS006	1809	GGTGATGCTACACCATTTAACGATGCTGT	31366154 (Toxoptera citricida)

CS006	1810	TCTCGTATGACAATTGGTCACTTGAT	49201759 (Drosophila melanogaster)

CS006	1811	CTGTCAACGTGCAGAAGATCTC	49573116 (Boophilus microplus)

CS007	1812	TGGATGAATGTGACAAAATGCTTGAA	84114516 (Blomia tropicalis)

CS007	1813	TTTATGCAAGATCCTATGGAAGT	84114516 (Blomia tropicalis)

CS007	1814	AAATTTATGCAAGATCCTATGGAAGTTTATGT	78525380 (Glossina morsitans)

CS007	1815	AATATGACTCAAGATGAGCGTCT	90137538 (Spodoptera frugiperda)

CS007	1816	ATGACTCAAGATGAGCGTCTCTCCCG	103792212 (Heliconius erato)

CS007	1817	ATGCAAGATCCTATGGAAGTTTA	77336752 (Chironomus tentans)

CS007	1818	ATGCAAGATCCTATGGAAGTTTATGT	77873166 (Aedes aegypti)

CS007	1819	CGCTATCAGCAGTTCAAAGATTTCCAGAAG	77873166 (Aedes aegypti)

CS007	1820	GAAAATGAAAAGAATAAGAAG	110759359 (Apis mellifera)
			78525380 (Glossina morsitans)

CS007	1821	GAAGTTCAACATGAATGTATTCC	110759359 (Apis mellifera)

CS007	1822	GATGAGCGTCTCTCCCGCTATCA	40932719 (Bombyx mori)

CS007	1823	TGCCAATTCAGAAAGATGAAGAAGT	110759359 (Apis mellifera)

CS007	1824	TGTAAGAAATTTATGCAAGATC	45244844 (Bombyx mori)

CS009	1825	AGGTGTGCGACGTGGACATCA	92460383 (Drosophila erecta)

CS009	1826	GACTTGAAGGAGCACATCAGGAA	29534871 (Bombyx mori)

CS009	1827	GGCCAGAACATCCACAACTGTGA	29534871 (Bombyx mori)

CS009	1828	TCTTGCGAGGGAGAGAATCCA	111005781 (Apis mellifera)

CS011	1829	AAAACTATTGTTTTCCACAGAAAAAAGAA	86465126 (Bombyx mori)

CS011	1830	ATCAAGGACAGAAAAGTCAAAGC	78230577 (Heliconius erato/himera mixed EST library)

CS011	1831	ATCTCTGCCAAGTCAAACTACAA	101406907 (Plodia interpunctella)

CS011	1832	CAATGTGCCATCATCATGTTCGA	110242457 (Spodoptera frugiperda)

CS011	1833	CCCAACTGGCACAGAGATTTAGTGCG	78230577 (Heliconius erato/himera mixed EST library)

CS011	1834	GACACTTGACTGGAGAGTTCGAGAAAAGATA	101410627 (Plodia interpunctella)

CS011	1835	GATATCAAGGACAGAAAAGTCAA	60312108 (Papillo dardanus)

CS011	1836	GCCAAGTCAAACTACAATTTCGA	67873076 (Drosophila pseudoobscura)

CS011	1837	GCTGGCCAAGAAAAGTTTGGTGGT	111031693 (Apis mellifera)

CS011	1838	GGCCAAGAAAAGTTTGGTGGTCTCCG	84267747 (Aedes aegypti)

CS011	1839	TACAAAAATGTACCCAACTGGCA	92963426 (Drosophila grimshawi)
			37951963 (Ips pini)

CS011	1840	TACAAAAATGTACCCAACTGGCACAGAGA	60312108 (Papilio dardanus)

CS011	1841	TATGGGATACTGCTGGCCAAGAA	40929360 (Bombyx mori)

CS011	1842	TATGGGATACTGCTGGCCAAGAAA	110749704 (Apis mellifera)

CS011	1843	TGGGATACTGCTGGCCAAGAA	73618835 (Aphis gossypii)
			112432160 (Myzus persicae)

CS011	1844	TGTGCCATCATCATGTTCGATGT	84346664 (Aedes aegypti)

CS011	1845	TTGACTGGAGAGTTCGAGAAA	90136305 (Bicyclus anynana)
			78230577 (Heliconius erato/himera mixed EST library)
			60312108 (Papilio dardanus)

CS011	1846	TTGACTGGAGAGTTCGAGAAAA	86465126 (Bombyx mori)
			110262261 (Spodoptera frugiperda)

CS011	1847	TGGGATACTGCTGGCCAAGAA	21639295 (Sarcoptes scabiei)

CS013	1848	GATCCCATTCAGTCTGTCAAGGG	3626535 (Drosophila melanogaster)

CS013	1849	TTCCAAGCAAAGATGTTGGATATGTTGAA	112433067 (Myzus persicae)

CS014	1850	AAAAAGATCCAATCTTCGAACATGCTGAA	103775905 (Heliconius erato)

CS014	1851	AAACAAGTGGAACTCCAGAAAAA	101403826 (Plodia interpunctella)

CS014	1852	AAAGTGCGTGAGGACCACGTACG	87266590 (Choristoneura fumiferana)
			3738660 (Manduca sexta)

CS014	1853	AAGATCAGCAACACTCTGGAGTC	58371699 (Lonomia obliqua)

CS014	1854	AAGATCAGCAACACTCTGGAGTCTCG	91848497 (Bombyx mori)

CS014	1855	AAGATCCAATCTTCGAACATG	77790417 (Aedes aegypti)

CS015	1883	CTGCCCCGATGAGAAGATCCGCATGAACCG	92948836 (Drosophila ananassae)

CS015	1884	GAGAAGATCCGCATGAACCGCGT	4691131 (Aedes aegypti)
			92466521 (Drosophila erecta)
			15070638 (Drosophila melanogaster)

CS015	1885	GTACATATATTGCCCATTGAT	90133859 (Bicyclus anynana)

CS015	1886	TCATCGCACGTGATCGTAATGGC	22474136 (Helicoverpa armigera)

CS015	1887	TTCATGGTTCGCGGGGGCATG	29551125 (Bombyx mori)

CS016	1888	AAATCGGTGTACATGTAACCTGGGAAACCACG	55797015 (Acyrthosiphon pisum)
			73615307 (Aphis gossypii)

CS016	1889	AAGTTGTCCTCGTGGTCGTCCA	91826756 (Bombyx mori)

CS016	1890	ACAGATCTGGGCGGCAATTTC	18950388 (Anopheles gambiae)
			31206154 (Anopheles gambiae str. PEST)

CS016	1891	ACAGCCTTCATGGCCTGCACGTCCTT	76169888 (Diploptera punctata)
			92953069 (Drosophila ananassae)
			92477149 (Drosophila erecta)
			8809 (Drosophila melanogaster)
			55694467 (Drosophila yakuba)

CS016	1892	ACATCAGAGTGGTCCTTGCGGGTCAT	55694467 (Drosophila yakuba)
			110248186 (Spodoptera frugiperda)

CS016	1893	ACCAGCACGTGTTTCTCACACTGGTA	91829127 (Bombyx mori)

CS016	1894	ACCTCCTCACGGGCGGCGGACAC	237458 (Heliothis virescens)
			27372076 (Spodoptera littoralis)

CS016	1895	ACGACAGCCTTCATGGCCTGCACGTCCTT	67896654 (Drosophila pseudoobscura)

CS016	1896	ACGTAGATCTGTCCCTCAGTGATGTA	53883819 (Plutella xylostella)

CS016	1897	AGAGCCTCCGCGTACGAAGACATGTC	53883819 (Plutella xylostella)

CS016	1898	AGCAATGGAGTTCATCACGTC	60295607 (Homalodisca coagulata)

CS016	1899	AGCAGCTGCCAGCCGATGTCCAG	92953069 (Drosophila ananassae)
			92477149 (Drosophila erecta)
			55694467 (Drosophila yakuba)
			112349870 (Helicoverpa armigera)
			237458 (Heliothis virescens)
			9713 (Manduca sexta)
			110242332 (Spodoptera frugiperda)

CS016	1900	AGCATCTCCTTGGGGAAGATACG	63005818 (Bombyx mori)
			92967975 (Drosophila mojavensis)
			92938364 (Drosophila virilis)
			92231646 (Drosophila willistoni)
			237458 (Heliothis virescens)

CS016	1901	AGGGCTTCCTCACCGACGACAGCCTTCATGGC	4680479 (Aedes aegypti)
		CTG

CS016	1902	ATACCAGTCTGGATCATTTCCTCAGG	60295607 (Homalodisca coagulata)

CS016	1903	ATACGGGACCAGGGGTTGATGGGCTG	92953552 (Drosophila ananassae)

CS016	1904	ATAGCGGAGATACCAGTCTGGATCAT	237458 (Heliothis virescens)
			76554661 (Spodoptera frugiperda)

CS016	1905	ATCTGGGCGGCAATTTCGTTGTG	83937869 (Lutzomyia longipalpis)

CS016	1906	ATGGCAGACTTCATGAGACGA	55894053 (Locusta migratoria)

CS016	1907	ATGGTGGCCAAATCGGTGTACATGTAACC	92965644 (Drosophila grimshawi)

CS016	1908	ATGGTGGCCAAATCGGTGTACATGTAACCT	92969578 (Drosophila grimshawi)

CS016	1909	ATGGTGGCCAAATCGGTGTACATGTAACCTGG	92231646 (Drosophila willistoni)
		GAAACCACG

CS016	1910	ATTCAAGAACAGGCACACGTTCTCCATGGAGCC	67841091 (Drosophila pseudoobscura)
		GTTCTCCTCGAAGTCCTGCTTGAAGAA

CS016	1911	ATTGGGGGACCTTTGTCAATGGGTTTTCC	49395165 (Drosophila melanogaster)
			99009492 (Leptinotarsa decemlineata)

CS016	1912	CACACGTTCTCCATGGAGCCGTTCTCCTCGAAG	92477818 (Drosophila erecta)
		TCCTGCTTGAAGAA

CS016	1913	CACTGGTAGGCCAAGAACTCAGC	4680479 (Aedes aegypti)

CS016	1914	CATCTCCTTGGGGAAGATACG	16899457 (Ctenocephalides felis)
			9713 (Manduca sexta)

CS016	1915	CCCTCACCGATGGCAGACTTCAT	4680479 (Aedes aegypti)
			92924977 (Drosophila virilis)
			110248186 (Spodoptera frugiperda)

CS016	1916	CCGATGGCAGACTTCATGAGACG	71049259 (Oncometopia nigricans)

CS016	1917	CCGTCTCCATGTTCACACCCATGGCGGCGAAC	33547658 (Anopheles gambiae)
		ACGATGGC

CS016	1918	CCGTTCTCCTCGAAGTCCTGCTTGAAGAA	31206154 (Anopheles gambiae str. PEST)
			8809 (Drosophila melanogaster)

CS016	1919	CCGTTCTCCTCGAAGTCCTGCTTGAAGAACC	101403557 (Plodia interpunctella)

CS016	1920	CGAGCAATGGAGTTCATCACGTCGATAGCGGA	27372076 (Spodoptera littoralis)
		GATACCAGTCTGGATCAT

CS016	1921	CGGGCCGTCTCCATGTTCACACCCATGGCGGC	31206154 (Anopheles gambiae str. PEST)
		GAACACGATGGC

CS016	1922	CGTCCGGGCACCTCCTCACGGGCGGC	18883474 (Anopheles gambiae)
			31206154 (Anopheles gambiae str. PEST)

CS016	1923	CGTCCGGGCACCTCCTCACGGGCGGCGGACAC	9713 (Manduca sexta)
			110248186 (Spodoptera frugiperda)

CS016	1924	CTACAGATCTGGGCGGCAATTTC	91826756 (Bombyx mori)
			9713 (Manduca sexta)
			27372076 (Spodoptera littoralis)

CS016	1925	CTACAGATCTGGGCGGCAATTTCGTTGTG	237458 (Heliothis virescens)
			76554661 (Spodoptera frugiperda)

CS016	1926	CTCGTAGATGGTGGCCAAATC	53883819 (Plutella xylostella)

CS016	1927	CTCGTAGATGGTGGCCAAATCGGTGTACATGTA	18883474 (Anopheles gambiae)
			31206154 (Anopheles gambiae str. PEST)

CS016	1928	CTCGTAGATGGTGGCCAAATCGGTGTACATGTA	92953069 (Drosophila ananassae)
		ACC	92477818 (Drosophila erecta)
			8809 (Drosophila melanogaster)
			67896654 (Drosophila pseudoobscura)

CS016	1929	CTCGTAGATGGTGGCCAAATCGGTGTACATGTA	9713 (Manduca sexta)
		ACCTGGGAAACCACG	110248186 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

CS016	1930	GAACAGGCACACGTTCTCCATGGA	92962756 (Drosophila ananassae)

CS016	1931	GACTCGAATACTGTGCGGTTCTCGTAGTT	87266757 (Choristoneura fumiferana)
			9713 (Manduca sexta)

CS016	1932	GACTTCATGAGACGAGACAGGGAAGGCAGCAC	9713 (Manduca sexta)
		GTT

CS016	1933	GAGATACCAGTCTGGATCATTTC	92969748 (Drosophila mojavensis)

CS016	1934	GAGATACCAGTCTGGATCATTTCCTC	92935139 (Drosophila virilis)

CS016	1935	GATGAAGTTCTTCTCGAACTTGG	2921501 (Culex pipiens)

CS016	1936	GATGAAGTTCTTCTCGAACTTGGT	4680479 (Aedes aegypti)
			31206154 (Anopheles gambiae str. PEST)
			92953069 (Drosophila ananassae)
			92477149 (Drosophila erecta)
			8809 (Drosophila melanogaster)
			67896654 (Drosophila pseudoobscura)
			55694467 (Drosophila yakuba)
			112349870 (Helicoverpa armigera)
			237458 (Heliothis virescens)

CS016	1937	GATGAAGTTCTTCTCGAACTTGGTGAGGAACTC	76555122 (Spodoptera frugiperda)
		GAGGTAGAGCA

CS016	1938	GATGGGGATCTGCGTGATGGA	101403557 (Plodia interpunctella)
			53883819 (Plutella xylostella)

CS016	1939	GCACACGTTCTCCATGGAGCCGTTCTC	104530890 (Belgica antarctica)

CS016	1940	GCCAAATCGGTGTACATGTAACCTGGGAAACCA	91829127 (Bombyx mori)
		CGTCGTCCGGG

CS016	1941	GCCAAGAACTCAGCAGCAGTCA	237458 (Heliothis virescens)

CS016	1942	GCCGTCTCCATGTTCACACCCA	83937868 (Lutzomyia longipalpis)

CS016	1943	GCCGTCTCCATGTTCACACCCAT	92965644 (Drosophila grimshawi)

CS016	1944	GCCTGCACGTCCTTACCGATGGCGTAGCA	112349870 (Helicoverpa armigera)
			237458 (Heliothis virescens)
			110248186 (Spodoptera frugiperda)

CS016	1945	GCCTTCATGGCCTGCACGTCCTT	39675733 (Anopheles gambiae)
			31206154 (Anopheles gambiae str. PEST)

CS016	1946	GCCTTCATGGCCTGCACGTCCTTACCGATGGC	2921501 (Culex pipiens)
		GTAGCA

CS016	1947	GCGGCGAACACGATGGCAAAGTT	2921501 (Culex pipiens)
			92965644 (Drosophila grimshawi)

CS016	1948	GCGGCGAACACGATGGCAAAGTTGTCCTCGTG	77905105 (Aedes aegypti)

CS016	1949	GCGTACAGCTGGTTGGAAACATC	67896654 (Drosophila pseudoobscura)

CS016	1950	GGAATAGGATGGGTGATGTCGTCGTTGGGCAT	110248186 (Spodoptera frugiperda)
		AGT

CS016	1951	GGAATAGGATGGGTGATGTCGTCGTTGGGCAT	27372076 (Spodoptera littoralis)
		AGTCA

CS016	1952	GGATGGGTGATGTCGTCGTTGGGCAT	101403557 (Plodia interpunctella)

CS016	1953	GGCAGACCGGCAGCCGAGAAAATGGGGATCTT	67841091 (Drosophila pseudoobscura)

CS016	1954	GGCATAGTCAAGATGGGGATCTG	92924977 (Drosophila virilis)

CS016	1955	GGCCGTCTCCATGTTCACACCCATGGC	101403557 (Plodia interpunctella)

CS016	1956	GGCGGGTAGATCTGTCTGTTGTG	2921501 (Culex pipiens)
			92965644 (Drosophila grimshawi)
			92924977 (Drosophila virilis)

CS016	1957	GGCGGGTAGATCTGTCTGTTGTGGAGCTGACG	237458 (Heliothis virescens)
		GTCTACGTAGATCTGTCCCTCAGT	110248186 (Spodoptera frugiperda)

CS016	1958	GGGAAGATACGGAGCAGCTGCCA	60336551 (Homalodisca coagulata)

CS016	1959	GGGTTGATGGGCTGTCCCTGGATGTCCAA	76554661 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

CS016	1960	GGTTTTCCAGAGCCGTTGAATAC	62238871 (Diabrotica virgifera)

CS016	1961	GTGATGAAGTTCTTCTCGAACTTGGT	87266757 (Choristoneura fumiferana)

CS016	1962	GTGCGGTTCTCGTAGTTGCCCTG	31206154 (Anopheles gambiae str. PEST)
			92477149 (Drosophila erecta)
			8809 (Drosophila melanogaster)
			67896654 (Drosophila pseudoobscura)
			92938364 (Drosophila virilis)
			92231646 (Drosophila willistoni)
			55694467 (Drosophila yakuba)

CS016	1963	GTGGCCAAATCGGTGTACATGTAACC	2921501 (Culex pipiens)
			75469507 (Tribolium castaneum)

CS016	1964	GTGTACATGTAACCTGGGAAACCACG	101403557 (Plodia interpunctella)

CS016	1965	GTGTACATGTAACCTGGGAAACCACGTCG	237458 (Heliothis virescens)

CS016	1966	GTGTACATGTAACCTGGGAAACCACGTCGTCC	53883819 (Plutella xylostella)
		GGGCACCTCCTCACGGGCGGC

CS016	1967	TCAGAGTGGTCCTTGCGGGTCAT	237458 (Heliothis virescens)
			9713 (Manduca sexta)

CS016	1968	TCAGCAAGGATTGGGGGACCTTTGTC	10763875 (Manduca sexta)

CS016	1969	TCCTCACCGACGACAGCCTTCATGGCCTG	92969578 (Drosophila grimshawi)

CS016	1970	TCCTCAGGGTAGATACGGGACCA	76554661 (Spodoptera frugiperda)

CS016	1971	TCCTCAGGGTAGATACGGGACCAGGGGTTGAT	22474040 (Helicoverpa armigera)
		GGGCTG	237458 (Heliothis virescens)
			9713 (Manduca sexta)

CS016	1972	TCGAAGTCCTGCTTGAAGAACC	9713 (Manduca sexta)

CS016	1973	TCGTAGATGGTGGCCAAATCGGTGTACATGTAA	62239897 (Diabrotica virgifera)
		CC

CS016	1974	TCGTAGATGGTGGCCAAATCGGTGTACATGTAA	4680479 (Aedes aegypti)
		CCTGGGAAACCACG

CS016	1975	TCTACGTAGATCTGTCCCTCAGTGATGTA	101403557 (Plodia interpunctella)

CS016	1976	TGCACGTCCTTACCGATGGCGTAGCA	9713 (Manduca sexta)
			75710699 (Tribolium castaneum)

CS016	1977	TGGGTGATGTCGTCGTTGGGCAT	53883819 (Plutella xylostella)

CS016	1978	TGGTAGGCCAAGAACTCAGCAGC	9713 (Manduca sexta)

CS016	1979	TTCAAGAACAGGCACACGTTCTCCAT	18883474 (Anopheles gambiae)
			31206154 (Anopheles gambiae str. PEST)
			92933153 (Drosophila virilis)
			27372076 (Spodoptera littoralis)

CS016	1980	TTCAAGAACAGGCACACGTTCTCCATGGA	92950254 (Drosophila ananassae)
			76554661 (Spodoptera frugiperda)

CS016	1981	TTCTCACACTGGTAGGCCAAGAA	18883474 (Anopheles gambiae)

CS016	1982	TTCTCCTCGAAGTCCTGCTTGAAGAA	83937868 (Lutzomyia longipalpis)

CS016	1983	TTGAGCATCTCCTTGGGGAAGATACG	92477149 (Drosophila erecta)
			8809 (Drosophila melanogaster)
			67896654 (Drosophila pseudoobscura)
			112349870 (Helicoverpa armigera)

CS016	1984	TTGAGCATCTCCTVGGGGAAGATACGGAGCA	83928466 (Lutzomyia longipalpis)

CS016	1985	TTGAGCATCTCCTTGGGGAAGATACGGAGCAG	50559098 (Homalodisca coagulata)
		CTGCCA	71049259 (Oncometopia nigricans)

CS016	1986	TTGAGCATCTCCTTGGGGAAGATACGGAGCAG	87266757 (Choristoneura fumiferana)
		CTGCCAGCCGATGTC

CS018	1987	TCCGACTACTCTTCCACGGAC	31659029 (Anopheles gambiae)

TABLE 4-PX

Target	SEQ
ID	ID NO	Sequence *	Example Gi-number and species

PX001	2120	AACAACGTGTTCATCATCGGCAAGGGCACGAA	112350001 (Helicoverpa armigera)

PX001	2121	AACGTGTTCATCATCGGCAAG	27562760 (Anopheles gambiae)
			58378595 (Anopheles gambiae str. PEST)

PX001	2122	AACGTGTTCATCATCGGCAAGG	42764924 (Armigeres subalbatus)

Px001	2123	AACGTGTTCATCATCGGCAAGGG	71048604 (Oncometopia nigricans)

PX001	2124	AACGTGTTCATCATCGGCAAGGGCACGAA	112783858 (Anopheles funestus)

PX001	2125	AACTTGGGGCGAGTGGGCACCATCGTGTC	90132259 (Bicyclus anynana)

PX001	2126	AACTTGGGGCGAGTGGGCACCATCGTGTCCCGCGAG	112350001 (Helicoverpa armigera)

PX001	2127	AAGATCGTGAAGCAGCGCCTCATCAAGGTGGACGGCAAGGT	112350001 (Helicoverpa armigera)

PX001	2128	AAGGTCCGCACCGACCCCACCTA	14627585 (Drosophila melanogaster)

PX001	2129	AAGTACAAGCTGTGCAAGGTG	5498893 (Antheraea yamamai)
			90132259 (Bicyclus anynana)
			92969396 (Drosophila grimshawi)
			50818668 (Heliconius melpomene)
			58371410 (Lonomia obliqua)

PX001	2130	ACAACGTGTTCATCATCGGCAAGGGCACGAA	103783745 (Heliconius erato)

PX001	2131	ACGGCAAGGTCCGCACCGACCC	77890923 (Aedes aegypti)

PX001	2132	ACGGCCGCACGCTGCGCTACCCCGACCCGCTCATCAAGGTC	101413238 (Plodia interpunctella)
		AACGACTCC

PX001	2133	ACGTGTTCATCATCGGCAAGGGCAC	109509107 (Culex pipiens)

PX001	2134	AGGAGGCCAAGTACAAGCTGT	27566312 (Anopheles gambiae)
			67889891 (Drosophila pseudoobscura)

PX001	2135	AGGAGGCCAAGTACAAGCTGTGCAAGGT	92944919 (Drosophila ananassae)
			67886177 (Drosophila pseudoobscura)
			92045792 (Drosophila willistoni)

PX001	2136	AGGAGGCCAAGTACAAGCTGTGCAAGGTG	92929731 (Drosophila virilis)

PX001	2137	CAACGTGTTCATCATCGGCAA	109509107 (Culex pipiens)

PX001	2138	CAACGTGTTCATCATCGGCAAGGGCA	55816641 (Drosophila yakuba)

PX001	2139	CACACCTTCGCCACCAGGUGAACAACGTGTT	3986403 (Antheraea yamamai)

PX001	2140	CCCCAAGAAGCATTTGAAGCG	2886669 (Drosophila melanogaster)

PX001	2141	CCGAGGAGGCCAAGTACAAGCT	92944919 (Drosophila ananassae)

PX001	2142	CCGAGGAGGCCAAGTACAAGCTGTGCAAGGT	15480750 (Drosophila melanogaster)

PX001	2143	CCGCACAAGCTGCGCGAGTGCCTGCCGCT	22474232 (Helicoverpa armigera)

PX001	2144	CGACGGGCCCCAAGAACGTGCC	112350001 (Helicoverpa armigera)

PX001	2145	CGAGGAGGCCAAGTACAAGCT	58378595 (Anopheles gambiae str. PEST)

PX001	2146	CGAGGAGGCCAAGTACAAGCTG	18914191 (Anopheles gambiae)

PX001	2147	CGAGTGGGCACCATCGTGTCCCGCGAG	3986403 (Antheraea yamamai)

PX001	2148	CGCTACCCCGACCCGCTCATCAAGGTCAACGACTCC	112350001 (Helicoverpa armigera)

PX001	2149	CGCTTCACCATCCACCGCATCAC	103783745 (Heliconius erato)

PX001	2150	CGGCAACGAGGTGCTGAAGATCGT	90132259 (Bicyclus anynana)

PX001	2151	CGTAACTTGGGGCGAGTGGGCAC	60311985 (Papilio dardanus)

PX001	2152	CTACCCGGCTGGATTCATGGATGT	42764924 (Armigeres subalbatus)

PX001	2153	CTCATCAAGGTCAACGACTCC	103783745 (Heliconius erato)

PX001	2154	CTCATCAAGGTCAACGACTCCATCCAGCTCGACAT	3738704 (Manduca sexta)

PX001	2155	GACGGCAAGGTCCGCACCGAC	109509107 (Culex pipiens)

PX001	2156	GACGGCAAGGTCCGCACCGACCC	77759638 (Aedes aegypti)

PX001	2157	GAGGAGGCCAAGTACAAGCTGTGCAAGGT	67841491 (Drosophila pseudoobscura)

PX001	2158	GAGGAGGCCAAGTACAAGCTGTGCAAGGTG	56772971 (Drosophila virilis)

PX001	2159	GAGGCCAAGTACAAGCTGTGCAA	112350001 (Helicoverpa armigera)

PX001	2160	GAGGCCAAGTACAAGCTGTGCAAGGTG	98993531 (Antheraea mylitta)

PX001	2161	GCCAAGTACAAGCTGTGCAAGGT	67838306 (Drosophila pseudoobscura)
			109978109 (Gryllus pennsylvanicus)

PX001	2162	GCCCCAAGAAGCATTTGAAGCG	2151718 (Drosophila melanogaster)

PX001	2163	GCGCGTGGCGACGGGCCCCAA	5498893 (Antheraea yamamai)

PX001	2164	GCGCGTGGCGACGGGCCCCAAG	3986403 (Antheraea yamamai)

PX001	2165	GGAGGCCAAGTACAAGCTGTGCAAGGT	92942537 (Drosophila ananassae)

PX001	2166	GGCCCCAAGAAGCATTTGAAGCG	4459798 (Drosophila melanogaster)

PX001	2167	GGCGGCGTGTACGCGCCGCGGCCC	98994282 (Antheraea mylitta)

PX001	2168	GTCCGCACCGACCCCACCTACCC	92472430 (Drosophila erecta)
			55854272 (Drosophila yakuba)

PX001	2169	GTGGGCACCATCGTGTCCCGCGAGAG	3953837 (Bombyx mandarina)
			29554802 (Bombyx mori)

PX001	2170	TCAAGGTGGACGGCAAGGTCCGCACCGACCC	92944919 (Drosophila ananassae)

PX001	2171	TGATCTACGATGTGAAGGGACG	83935965 (Lutzomyia longipalpis)

PX001	2172	TTCATGGATGTTGTGTCGATTGAAAA	90132259 (Bicyclus anynana)

PX001	2173	GCTGGATTCATGGATGTTGTG	10707240 (Amblyomma americanum)

PX001	2174	AAGCAGCGCCTCATCAAGGTGGACGGCAAGGTCCGCACCGAC	49545866 (Rhipicephalus appendiculatus)

PX009	2175	AACATCTTCAACTGTGACTTC	93001544 (Drosophila mojavensis)

PX009	2176	TGATCAACATCGAGTGCAAAGC	110755556 (Apis mellifera)

PX009	2177	TTCTTGAAGCTGAATAAGATCT	103750396 (Drosophila melanogaster)

PX010	2178	CAGTTCCTGCAGGTCTTCAACAA	71553175 (Oncometopia nigricans)

PX010	2179	CCATCAGCGGACGGTGGCGCCCCCGTG	90139187 (Spodoptera frugiperda)

PX010	2180	CCCGCAGTTCATGTACCACCTGCGCCGCTCGCAGTTC	67893194 (Drosophila pseudoobscura)

PX010	2181	CCGAACAGCTTCCGTCTGTCGGAGAACTTCAG	29558345 (Bombyx mori)

PX010	2182	CGCCTGTGCCAGAAGTTCGGCGAGTACG	58395529 (Anopheles gambiae str. PEST)

PX010	2183	CTGCGCCGCTCGCAGTTCCTGCAGGT	18872210 (Anopheles gambiae)

PX010	2184	CTGTACCCGCAGTTCATGTACCA	29558345 (Bombyx mori)

PX010	2185	GACGTGCTGCGCTGGCTCGACCG	29558345 (Bombyx mari)

PX010	2186	GACGTGTCGCTGCAAGTGTTCATGGAGCA	18872210 (Anopheles gambiae)

PX010	2187	GAGTACGAGAACTTCAAGCAGCTGCTGC	77886140 (Aedes aegypti)
			18872210 (Anopheles gambiae)
			49376735 (Drosophila melanogaster)
			67893324 (Drosophila pseudoobscura)

PX010	2188	GGCGGGGCGATGCCGATACCATC	91757875 (Bombyx mori)

PX010	2189	GTGGCTGCATACAGTTCATTACGCAGTACCAGCAC	28571527 (Drosophila melanogaster)

PX010	2190	TCGCAGTTCCTGCAGGTCTTCAACAA	92932090 (Drosophila virilis)

PX010	2191	TGCGCCGCTCGCAGTTCCTGCAGGTCTTCAACAA	67893324 (Drosophila pseudoobscuna)

PX010	2192	TGCGCCGCTCGCAGTTCCTGCAGGTCTTCAACAACTCGCCC	92952825 (Drosophila ananassae)
		GACGAGACCAC

PX010	2193	TTCATGTACCACCTGCGCCGCTCGCAGTTCCTGCAGGTCTTC	28571527 (Drosophila melanogaster)
		AACAACTCGCCCGACGAGACCAC

PX010	2194	ATCCTGCTCATGGACACCTTCTTCCA	82842646 (Boophilus microplus)

PX015	2195	CACCGCGACGACACGTTCATGGTGCGCGGCGG	58371643 (Lonomia obliqua)

PX015	2196	CAGATCAAGGAGATGGTGGAG	92480997 (Drosophila erecta)
			58371722 (Lonomia oblique)

PX015	2197	CCCGACGAGAAGATCCGCATGAA	67873606 (Drosophila pseudoobscura)

PX015	2198	CCCGACGAGAAGATCCGCATGAACCGCGT	15070733 (Drosophila melanogaster)

PX015	2199	CCGACGAGAAGATCCGCATGAACCGCGT	92459970 (Drosophila erecta)

PX015	2200	CGCAAGGAGACCGTGTGCATTGTGCT	67873606 (Drosophila pseudoobscura)

PX015	2201	GACGAGAAGATCCGCATGAACCG	18914444 (Anopheles gambiae)

PX015	2202	GACGAGAAGATCCGCATGAACCGCGT	4691131 (Aedes aegypti)

PX015	2203	GCGCAGATCAAGGAGATGGTGGAGCT	99007898 (Leptinotarsa decemlineata)

PX015	2204	GGCATGCGCGCCGTCGAGTTC	6901917 (Bombyx mori)

PX015	2205	GTGCGCGGCGGCATGCGCGCC	67891252 (Drosophila pseudoobscura)

PX015	2206	TCAAGGAGATGGTGGAGCTGC	27819993 (Drosophila melanogaster)

PX015	2207	TGAAGCCGTACTTCATGGAGGC	29559940 (Bombyx mori)

PX015	2208	TGCCGCAAGCAGCTGGCGCAGATCAAGGAGATGGT	18914444 (Anopheles gambiae)

PX015	2209	TGGAGGCGTACCGGCCCATCCAC	18914444 (Anopheles gambiae)

PX016	2210	AAGGACCACTCCGACGTGTCCAA	101406307 (Plodia interpunctella)

PX016	2211	AAGGACGTGCAGGCGATGAAGGC	112349870 (Helicoverpa armigera)
			110248186 (Spodoptera frugiperda)

PX016	2212	ACCAAGTTCGAGAAGAACTTCATC	4680479 (Aedes aegypti)
			31206154 (Anopheles gambiae str. PEST)
			92953069 (Drosophila ananassae)
			92477149 (Drosophila erecta)
			24646340 (Drosophila melanogaster)
			67900295 (Drosophila pseudoobscura)
			55694467 (Drosophila yakuba)
			112349870 (Helicoverpa armigera)
			237458 (Heliothis virescens)

PX016	2213	ACCAAGTTCGAGAAGAACTTCATCAC	87266757 (Choristoneura fumiferana)

PX016	2214	ACCGCCAGGTTCTUCAAGCAGGACTTCGA	9713 (Manduca sexta)

PX016	2215	ACCGGCGATATTCTGCGCACGCCCGTCTC	92940287 (Drosophila virilis)

PX016	2216	AGCAGGACTTCGAGGAGAACGG	67880606 (Drosophila pseudoobscura)

PX016	2217	ATCACGCAGATCCCCATCCTGACCATGCC	31206154 (Anopheles gambiae str. PEST)

PX016	2218	ATCTTGACCGACATGTCTTCATACGC	104530890 (Belgica antarctica)
			92231646 (Drosophila willistoni)

PX016	2219	ATGACCAGGAAGGACCACTCCGACGT	75713096 (Tribolium castaneum)

PX016	2220	ATGCCCAACGACGACATCACCCA	101406307 (Plodia interpunctella)
			76555122 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

PX016	2221	CAGAAGATCCCCATCTTCTCCGCCGCCGGTCTGCCCCACAA	92460896 (Drosophila erecta)
		CGA	24646340 (Drosophila melanogaster)

PX016	2222	CAGGACTTCGAGGAGAACGGTTCCATGGAGAACGT	2921501 (Culex pipiens)
			76554661 (Spodoptera frugiperda)

PX016	2223	CCAAGTTCGAGAAGAACTTCATC	2921501 (Culex pipiens)

PX016	2224	CCCATCAACCCGTGGTCCCGTATCTACCCGGAGGA	2921501 (Culex pipiens)

PX016	2225	CCCGACTTGACCGGGTACATCACTGAGGGACAGATCTACGT	101406307 (Plodia interpunctella)

PX016	2226	CCCGGACGACGTGGTTUCCCAGGTTACATGTACAC	91829127 (Bombyx mori)

PX016	2227	CCTGGACATCCAGGGGCAGCCCATCAACCC	91090030 (Tribolium castaneum)

PX016	2228	CGACGTGGTTTCCCAGGTTACATGTACACGGATTTGGC	237458 (Heliothis virescens)

PX016	2229	CGTCTCATGAAGTCCGCCATCGG	91829127 (Bombyx mori)

PX016	2230	CGTCTCATGAAGTCCGCCATCGGAGAGGGCATGACC	237458 (Heliothis virescens)

PX016	2231	CGTGGTCAGAAGATCCCCATCTTCTC	27372076 (Spodoptera littoralis)

PX016	2232	CGTGGTCAGAAGATCCCCATCTTCTCCGC	76554661 (Spodoptera frugiperda)

PX016	2233	CGTGGTTTCCCAGGTTACATGTACAC	55797015 (Acyrthosiphon pisum)
			4680479 (Aedes aegypti)
			73615307 (Aphis gossypii)
			92231646 (Drosophila willistoni)
			9713 (Manduca sexta)
			76555122 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

PX016	2234	CGTGGTTTCCCAGGTTACATGTACACGGATTTGGCCACAATC	101406307 (Plodia interpunctella)
		TACGAGCGCGCCGGGCG

PX016	2235	CTACGAGAACCGCACAGTGTTCGAGTC	112350031 (Helicoverpa armigera)
			237458 (Heliothis virescens)
			76555122 (Spodoptera frugiperda)

PX016	2236	CTGCGTATCTTCCCCAAGGAGAT	63005818 (Bombyx mori)
			92477149 (Drosophila erecta)
			24646340 (Drosophila melanogaster)
			56773982 (Drosophila pseudoobscura)
			92935600 (Drosophila vinilis)
			92220609 (Drosophila willistoni)
			112350031 (Helicoverpa armigera)
			237458 (Heliothis virescens)
			9713 (Manduca sexta)

PX016	2237	CTGTACGCGTGCTACGCCATCGG	9713 (Manduca sexta)

PX016	2238	CTGTTCTTGAACTTGGCCAATGA	16898595 (Ctenocephalides felis)

PX016	2239	CTGTTCTTGAACTTGGCCAATGACCC	27372076 (Spodoptera littoralis)

PX016	2240	GACAACTTCGCCATCGTGTTCGC	92950254 (Drosophila ananassae)

PX016	2241	GACAACTTCGCCATCGTGTTCGCCGC	92477818 (Drosophila erecta)
			24646340 (Drosophila melanogaster)
			237458 (Heliothis virescens)
			9713 (Manduca sexta)
			76554661 (Spodoptera frugiperda)

PX016	2242	GACAACTTCGCCATCGTGTTCGCCGCCATGGG	31206154 (Anopheles gambiae str. PEST)

PX016	2243	GACCGTCAGCTGCACAACAGGCA	50564193 (Homalodisca coagulata)

PX016	2244	GACCTGCTCTACCTCGAGTTC	112349870 (Helicoverpa armigera)

PX016	2245	GACGTGATGAACTCCATCGCCCG	237458 (Heliothis virescens)

PX016	2246	GACGTGATGAACTCCATCGCCCGTGG	22474040 (Helicoverpa armigera)

PX016	2247	GAGAACGGTTCCATGGAGAACGT	91829127 (Bombyx mori)

PX016	2248	GAGGAGATGATCCAGACTGGTATCTCCGCTAT	237458 (Heliothis virescens)
			76554661 (Spodoptera frugiperda)

PX016	2249	GAGGAGATGATCCAGACTGGTATCTCCGCTATCGACGTGATG	27372076 (Spodoptera littoralis)
		AACTCCAT

PX016	2250	GAGGAGGCGCTCACGCCCGACGAC	9713 (Manduca sexta)

PX016	2251	GAGTTCTTGGCCTACCAGTGCGAGAA	4680479 (Aedes aegypti)

PX016	2252	GCCAGGTTCTTCAAGCAGGACTTCGAGGAGAACGG	101403557 (Plodia interpunctella)

PX016	2253	GCCCGTGGTCAGAAGATCCCCAT	67877903 (Drosophila pseudoobscura)

PX016	2254	GCCCGTGGTCAGAAGATCCCCATCTTCTC	6901845 (Bombyx mori)

PX016	2255	GCCCGTGGTCAGAAGATCCCCATCTTCTCCGCCGC	92950254 (Drosophila ananassae)

PX016	2256	GCCGAGTTCTTGGCCTACCAGTGCGAGAA	24646340 (Drosophila melanogaster)

PX016	2257	GCCGAGTTCTTGGCCTACCAGTGCGAGAAACACGTGTTGGT	110240379 (Spodoptera frugiperda)

PX016	2258	GCCGCCCGTGAGGAGGTGCCCGGACG	31206154 (Anopheles gambiae str. PEST)
			9713 (Manduca sexta)
			110240379 (Spodoptera frugiperda)

PX016	2259	GCCTACCAGTGCGAGAAACACGTGTTGGTAATCTTGACCGAC	101406307 (Plodia interpunctella)
		ATGTC

PX016	2260	GGCAGATCTACCCGCCGGTGAA	31206154 (Anopheles gambiae str. PEST)

PX016	2261	GGCGAGGAGGCGCTCACGCCCGACGA	31206154 (Anopheles gambiae str. PEST)

PX016	2262	GGTCAGAAGATCCCCATCTTCTC	60295607 (Homalodisca coagulata)

PX016	2263	GGTTACATGTACACGGATTTGGCCAC	92924977 (Drosophila virilis)

PX016	2264	GTGGTGGGCGAGGAGGCGCTCACGCC	112349870 (Helicoverpa armigera)

PX016	2265	GTTCACCGGCGATATTCTGCG	92997483 (Drosophila grimshawi)

PX016	2266	GTTCACCGGCGATATTCTGCGCAC	92950254 (Drosophila ananassae)
			92048971 (Drosophila willistoni)

PX016	2267	TACCAGTGCGAGAAACACGTGTTGGT	237458 (Heliothis virescens)

PX016	2268	TACGCCATCGGCAAGGACGTGCAGGCGATGAAGGC	87266757 (Choristoneura fumiferana)

PX016	2269	TCCATCACGCAGATCCCCATCCT	101406307 (Plodia interpunctella)

PX016	2270	TCCGGCAAGCCCATCGACAAGGG	92460896 (Drosophila erecta)
			24646340 (Drosophila melanogaster)
			22474040 (Helicoverpa armigera)
			237458 (Heliothis virescens)

PX016	2271	TCTACGAGCGCGCCGGGCGAGTC	33528180 (Trichoplusia ni)

PX016	2272	TCTCGTCTCATGAAGTCCGCCATCGG	9713 (Manduca sexta)

PX016	2273	TGACTGCTGCCGAGTTCTTGGCCTACCAGTGCGAGAAACAC	27372076 (Spodoptera littoralis)
		GTGTTGGT

PX016	2274	TGCACAACAGGCAGATCTACCC	62239897 (Diabrotica virgifera)

PX016	2275	TGCGTATCTTCCCCAAGGAGAT	16900620 (Ctenocephalides felis)
			92967975 (Drosophila mojavensis)

PX016	2276	TGCTACGCCATCGGCAAGGACGTGCAGGC	31206154 (Anopheles gambiae str. PEST)
			92953069 (Drosophila ananassae)
			92477149 (Drosophila erecta)
			24646340 (Drosophila melanogaster)
			67898824 (Drosophila pseudoobscura)
			55694467 (Drosophila yakuba)

PX016	2277	TGCTCTACCTCGAGTTCCTCACCAAGTTCGAGAAGAACTTCA	76555122 (Spodoptera frugiperda)
		TC

PX016	2278	TGTCTGTTCTTGAACTTGGCCAA	4680479 (Aedes aegypti)
			92477818 (Drosophila erecta)
			24646340 (Drosophila melanogaster)

PX016	2279	TGTCTGTTCTTGAACTTGGCCAATGA	55905051 (Locusta migratoria)

PX016	2280	TGTTCTTGAACTTGGCCAATGA	91090030 (Tribolium castaneum)

PX016	2281	TTCAACGGCTCCGGCAAGCCCAT	76554661 (Spodoptera frugiperda)

PX016	2282	TTCAACGGCTCCGGCAAGCCCATCGACAAGGG	4680479 (Aedes aegypti)
			31206154 (Anopheles gambiae str. PEST)
			67877903 (Drosophila pseudoobscura)

PX016	2283	TTCGAGGAGAACGGTTCCATGGAGAA	92972277 (Drosophila grimshawi)

PX016	2284	TTCGAGGAGAACGGTTCCATGGAGAACGT	92950254 (Drosophila ananassae)

PX016	2285	TTCTTCAAGCAGGACTTCGAGGAGAA	83937868 (Lutzomyia longipalpis)

PX016	2286	TTCTTCAAGCAGGACTTCGAGGAGAACGG	92477818 (Drosophila erecta)

PX016	2287	TTCTTCAAGCAGGACTTCGAGGAGAACGGTTC	31206154 (Anopheles gambiae str. PEST)

PX016	2288	TTCTTCAAGCAGGACTTCGAGGAGAACGGTTCCATGGAGAAC	24646340 (Drosophila melanogaster)
		GT

PX016	2289	TTCTTGAACTTGGCCAATGACCC	9713 (Manduca sexta)

PX016	2290	TTCTTGGCCTACCAGTGCGAGAA	31206154 (Anopheles gambiae str. PEST)
			67883622 (Drosophila pseudoobscura)
			92231646 (Drosophila willistoni)

TABLE 4-AD

Target	SEQ ID
ID	NO	Sequence *	Example Gi-number and species

AD001	2384	AAAGCATGGATGTTGGACAAA	73619372 (Aphis gossypii);
			77325485 (Chironomus tentans);
			22474232 (Helicoverpa armigera);
			37951951 (Ips pini);
			60305420 (Mycetophagus quadripustulatus);
			84647995 (Myzus persicae)

AD001	2385	AAAGCATGGATGTTGGACAAACT	94432102 (Bombyx mori);
			103790417 (Heliconius erato);
			55904580 (Locusta migratoria);
			101419954 (Plodia interpunctella)

AD001	2386	AAAGGTATTCCATTCTTGGTGACCCATGATGGCC	109978109 (Gryllus pennsylvanicus)
		GTACTATCCGTTATCCTGACCCAGTCATTAAAGT

AD001	2387	AACTGTGAAGTAACGAAGATTGTTATGCAGCGACT	109978109 (Gryllus pennsylvanicus)
		TATCAAAGTTGA

AD001	2388	AAGAAGCATTTGAAGCGTTTAAA	3658572 (Manduca sexta)

AD001	2389	AAGGGTAAGGGTGTGAAATTGAGTAT	109978109 (Gryllus pennsylvanicus)

AD001	2390	AATGTATTCATCATTGGAAAAGC	55904577 (Locusta migratoria)

AD001	2391	AGAAGCATTTGAAGCGTTTAAA	98994282 (Antheraea mylitta)
			73619372 (Aphis gossypii)

AD001	2392	AGAAGCATTTGAAGCGTTTAAATGC	27620566 (Anopheles gambiae)

AD001	2393	AGTACTGGCCCCCACAAATTGCG	109978109 (Gryllus pennsylvanicus)

AD001	2394	AGTGCAGAAGAAGCCAAGTACAAGCT	109978109 (Gryllus pennsylvanicus)

AD001	2395	ATCGCCGAGGAGCGGGACAAGC	3953837 (Bombyx mandarina)
			94432102 (Bombyx mori)

AD001	2396	CAAGGACATACTTTTGCCACAAGATTGAATAATGT	109978109 (Gryllus pennsylvanicus)
		ATTCATCATTGGAAA

AD001	2397	CAGAAGAAGCCAAGTACAAGCT	42764924 (Armigeres subalbatus)

AD001	2398	CATGATGGCCGTACTATCCGTTA	73613065 (Aphis gossypii)

AD001	2399	CATGATGGCCGTACTATCCGTTATCCTGACCC	31365398 (Toxoptera citricida)

AD001	2400	CATTTGAAGCGTTTAAATGCTCC	27557322 (Anopheles gambiae)

AD001	2401	CCTAAAGCATGGATGTTGGAC	77324536 (Chironomus tentans)

AD001	2402	CCTAAAGCATGGATGTTGGACAA	58371410 (Lonomia obliqua)

AD001	2403	CCTAAAGCATGGATGTTGGACAAA	60311985 (Papilio dardanus)
			30031258 (Toxoptera citricida)

AD001	2404	CCTAAAGCATGGATGTTGGACAAACT	98994282 (Antheraea mylitta)

AD001	2405	CGTACTATCCGTTATCCTGACCC	37804548 (Rhopalosiphum padi)

AD001	2406	GAATGTTTACCTTTGGTGATTTTTCTTCGCAATCG	109978109 (Gryllus pennsylvanicus)
		GCT

AD001	2407	GCAGAAGAAGCCAAGTACAAGCT	37953169 (Ips pini)

AD001	2408	GCATGGATGTTGGACAAACTCGG	83935968 (Lutzomyia longipalpis)

AD001	2409	GCTGGTTTCATGGATGTTGTCAC	109978109 (Gryllus pennsylvanicus)

AD001	2410	GGCCCCAAGAAGCATTTGAAGCGTTTAA	14693528 (Drosophila melanogaster)

AD001	2411	GGTTTCATGGATGTTGTCACCAT	25958683 (Curculio glandium)

AD001	2412	TATGATGTGAAAGGCCGTTTCACAATTCACAGAAT	109978109 (Gryllus pennsylvanicus)

AD001	2413	TCATTGCCAAAGGGTAAGGGT	77324972 (Chironomus tentans)

AD001	2414	TGGATATTGCCACTTGTAAAATCATGGACCACATC	109978109 (Gryllus pennsylvanicus)
		AGATTTGAATCTGG

AD001	2415	TTAAATGCTCCTAAAGCATGGATGTTGGACAAACT	109978109 (Gryllus pennsylvanicus)

AD001	2416	TTTGAATCTGGCAACCTGTGTATGAT	60311985 (Papilio dardanus)

AD001	2417	TTTGATATTGTTCATATCAAGGATAC	109978109 (Gryllus pennsylvanicus)

AD002	2418	AAGAAAATCGAACAAGAAATC	55902553 (Locusta migratoria)

AD002	2419	CAGCACATGGATGTGGACAAGGT	67899569 (Drosophila pseudoobscura)

AD002	2420	GAGTTTCTTTAGTAAAGTATTCGGTGG	110762684 (Apis mellifera)

AD009	2421	CACTACAACTACCACAAGAGC	84226228 (Aedes aegypti)
			18941376 (Anopheles gambiae)

AD009	2422	CAGAACATCCACAACTGTGACT	29534871 (Bombyx mori)

AD009	2423	GGTGTGGGTGTCGTGCGAGGG	83926368 (Lutzomyia longipalpis)

AD009	2424	TGGATCCCTGAATACTACAATGA	83926506 (Lutzomyia longipalpis)

AD015	2425	GAGCAGTAGAATTCAAAGTAGT	99012451 (Leptinotarsa decemlineata)

AD015	2426	GCAATTATATTTATTGATGAA	83936542 (Lutzomyia longipalpis)

AD015	2427	TCACCATATTGTATTGTTGCT	31366806 (Toxoptera citricida)

AD015	2428	TTGTCCTGATGTTAAGTATGG	84114691 (Blomia tropicalis)

AD016	2429	ACGATGCCCAACGACGACATCACCCATCC	101406307 (Plodia interpunctella)

AD016	2430	ATGCCCAACGACGACATCACCCA	53883819 (Plutella xylostella)

AD016	2431	ATGCCCAACGACGACATCACCCATCCTATT	110240379 (Spodoptera frugiperda)
			27372076 (Spodoptera littoralis)

AD016	2432	CAGAAGATCCCCATCTTCTCGG	91827264 (Bombyx mori)
			22474331 (Helicoverpa armigera)
			60295607 (Homalodisca coagulata)

AD016	2433	CGGCTCCATCACTCAGATCCCCAT	67896654 (Drosophila pseudoobscura)

AD016	2434	GCCAACGACCCCACCATCGAG	101406307 (Plodia interpunctella)

AD016	2435	GCCCGTGTCCGAGGACATGCTGGG	83937868 (Lutzomyia longipalpis)
			75473525 (Tribolium castaneum)

AD016	2436	GGCAGAAGATCCCCATCTTCTC	2286803 (Drosophila melanogaster)

AD016	2437	GTTCACCGGCGATATTCTGCG	92997483 (Drosophila grimshawi)

AD016	2438	GTTCACCGGCGATATTCTGCGC	92953552 (Drosophila ananassae)
			92042621 (Drosophila willistoni)

TABLE 5-LD

Target ID	SEQ ID No	Sequences*	Example Gi-number and species

LD001	124	AAGAAGCATTTGAAGCGTTTG	8005678 (Meloidogyne incognita),
			9829015 (Meloidogyne javanica)

LD003	125	GTTCTTCCTCTTGACGCGTCC	7710484 (Zeldia punctata)

LD003	126	GCAGCTTTACGGATTTTTGCCAA	32183696 (Meloidogyne chitwoodi)

LD003	127	TTTCAACTCCTGATCAAGACGT	1662318 (Brugia malayi),
			31229562 (Wuchereria bancrofti)

LD006	128	GCTATGGGTAAGCAAGCTATGGG	520506 (Caenorhabditis elegans)

LD007	129	AAAGAATAAAAAATTATTTGA	17539725 (Caenorhabditis elegans)

LD007	130	AAGCAAGTGATGATGTTCAGTGC	7143515 (Globodera pallida)

LD014	131	ATGATGGCTTTCATTGAACAAGA	10122191 (Haemonchus contortus)

LD015	132	AACGCCCCAGTCTCATTAGCCAC	20064339 (Meloidogyne hapla)

LD016	133	TTTTGGCGTCGATTCCTGATG	71999357 (Caenorhabditis elegans)

LD016	134	GTGTACATGTAACCTGGGAAACC	13418283 (Necator americanus)

LD016	135	GTGTACATGTAACCTGGGAAACCACGACG	10819046 (Haemonchus contortus)

TABLE 5-PC

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

PC001	435	ATGGATGTTGGACAAATTGGG	7143612 (Globodera rostochiensis)

PC003	436	GCTAAAATCCGTAAAGCTGCTCGTGAACT	9831177 (Strongyloides stercoralis)

PC003	437	GAGTAAAGTACACTTTGGCTAAA	28914459 (Haemonchus contortus)

PC003	438	AAAATCCGTAAAGCTGCTCGTGAACT	32185135 (Meloidogyne chitwoodi)

PC003	439	CTGGACTCGCAGAAGCACATCGACTT	51334250 (Radopholus similis)

PC003	440	CGTCTGGATCAGGAATTGAAA	61115845 (Litomosoides sigmodontis)

PC005	441	TGGTTGGATCCAAATGAAATCAA	5430825 (Onchocerca volvulus)

PC005	442	GTGTGGTTGGATCCAAATGAAATCAA	6845701 (Brugia malayi);
			45215079 (Wuchereria bancrofti)

PC014	443	CACATGATGGCTTTCATTGAACAAGAAGC	10122191 (Haemonchus contortus)

PC014	444	TACGAGAAAAAGGAGAAGCAAGT	21265518 (Ostertagia ostertagi)

PC016	445	GTCTGGATCATTTCCTCGGGATAAAT	18081287 (Globodera rostochiensis)

PC016	446	CCAGTCTGGATCATTTCCTCGGGATA	108957716 (Bursaphelenchus mucronatus);
			108962248 (Bursaphelenchus xylophilus)

TABLE 5-EV

			Example
Target	SEQ ID		Gi-number
ID	NO	Sequence *	and species

EV005	563	TTAAAGATGGTCTTATTATTAA	21819186
			(Trichinella
			spiralis)

EV016	564	GCTATGGGTGTCAATATGGAAAC	54554020
			(Xiphinema
			index)

TABLE 5-AG

Target ID	SEQ ID NO	Sequence *	Example Gi-number and species

AG001	739	GCTGGATTCATGGATGTGATCA	15666884 (Ancylostoma ceylanicum)

AG001	740	ATGGATGTTGGACAAATTGGG	18081843 (Globodera rostochiensis)

AG001	741	TTCATGGATGTGATCACCATTGA	27002091 (Ascaris suum)

AG005	742	GTCTGGTTGGATCCAAATGAAATCAATGA	2099126 (Onchocerca volvulus)

AG005	743	GGATCCAAATGAAATCAATGA	2099309 (Onchocerca volvulus)

AG005	744	TGATCAAGGATGGTTTGATCAT	2130916 (Brugia malayi)

AG005	745	TGGTTGGATCCAAATGAAATCAATGA	6845701 (Brugia malayi)

AG005	746	CCAAGGGTAACGTGTTCAAGAACAAG	29964728 (Heterodera glycines)

AG005	747	TGGTTGGATCCAAATGAAATCAATGA	45215079 (Wuchereria bancrofti)

AG005	748	TGGATCCAAATGAAATCAATGA	61116961 (Litomosoides sigmodontis)

AG014	749	GAAGAATTTAACATTGAAAAGGG	10122191 (Haemonchus contortus)

AG014	750	GAATTTAACATTGAAAAGGGCCG	28252967 (Trichuris vulpis)

AG016	751	GGTTACATGTACACCGATTTGGC	54552787 (Xiphinema index)

TABLE 5-TC

	SEQ		Example
Target	ID		Gi-number
ID	NO	Sequence *	and species

TC014	853	ATCATGGAATATTACGAGAAGAA	6562543
			(Heterodera
			schachtii);
			15769883
			(Heterodera
			glycines)

TC015	854	AACGGTCCCGAAATTATGAGTAAA	108966476
		TT	(Bursaphelenchus
			xylophilus)

TABLE 5-MP

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

MP001	1011	GATCTTTTGATATTGTTCACATTAA	13099294 (Strongyloides ratti)

MP001	1012	ACATCCAGGATCTTTTGATATTGTTCAC	15275671 (Strongyloides ratti)

MP001	1013	TCTTTTGATATTGTTCACATTAA	32183548 (Meloidogyne
			chitwoodi)

MP016	1014	TATTGCTCGTGGACAAAAAAT	9832367 (Strongyloides
			stercoralis)

MP016	1015	TCTGCTGCTCGTGAAGAAGTACCTGG	13418283 (Necator americanus)

MP016	1016	GCTGAAGATTATTTGGATATT	20064440 (Meloidogyne hapla)

MP016	1017	GGTTTACCACATAATGAGATTGCTGC	20064440 (Meloidogyne hapla)

MP016	1018	AAGAAATGATTCAAACTGGTATTTCAGCTATTGAT	31545172 (Strongyloides ratti)

MP016	1019	TATTGCTCGTGGACAAAAAATTCCAAT	31545172 (Strongyloides ratti)

MP016	1020	GTTTCTGCTGCTCGTGAAGAAGT	31545172 (Strongyloides ratti)

MP016	1021	CGTGGTTTCCCTGGTTACATGTACAC	31545172 (Strongyloides ratti)

MP016	1022	CCTGGTTACATGTACACCGATTT	54552787 (Xiphinema index)

MP027	1023	TTTAAAAATTTTAAAGAAAAA	27540724 (Meloidogyne hapla)

MP027	1024	CTATTATGTTGGTGGTGAAGTTGT	34026304 (Meloidogyne
			arenaria)

MP027	1025	AAAGTTTTTAAAAATTTTAAA	34028558 (Meloidogyne
			javanica)

TABLE 5-NL

Target ID	SEQ ID No	Sequence*	Example Gi-number and species

NL001	1438	AGTACAAGCTGTGCAAAGTGAAGA	18087933 (Globodera rostochiensis), 54547517
			(Globodera pallida)

NL001	1439	ATGGATGTTGGACAAATTGGGTGG	7143612 (Globodera rostochiensis)

NL001	1440	TGGATGTTGGACAAATTGGGTGG	7235910 (Meloidogyne incognita)

NL001	1441	AGTACAAGCTGTGCAAAGTGAAGA	111164813 (Globodera rostochiensis)

NL003	1442	AGTCCATCCATCACGCCCGTGT	6081031 (Pristionchus pacificus)

NL003	1443	CTCCGTAACAAGCGTGAGGTGTGG	5815927 (Pristionchus pacificus)

NL003	1444	GACTCGCAGAAGCACATTGACTTCTC	5815618 (Pristionchus pacificus)

NL003	1445	GCAGAAGCACATTGACTTCTC	6081031 (Pristionchus pacificus)

NL003	1446	GCCAAGTCCATCCATCACGCCC	6081133 (Pristionchus pacificus)

NL003	1447	GCCAAGTCCATCCATCACGCCCGTGT	1783663 (Pristionchus pacificus)

NL003	1448	TCGCAGAAGCACATTGACTTCTC	10804008 (Ascaris suum)

NL003	1449	TCGCAGAAGCACATTGACTTCTCGCTGAA	18688500 (Ascaris suum)

NL003	1450	GCCAAGTCCATCCATCACGCCCGTGT	91102596 (Pristionchus pacificus)

NL003	1451	GACTCGCAGAAGCACATTGACTTCTC	91102596 (Pristionchus pacificus)

NL003	1452	CTCCGTAACAAGCGTGAGGTGTGG	91102596 (Pristionchus pacificus)

NL004	1453	AAGAACAAGGATATTCGTAAATT	3758529 (Onchocerca volvulus), 6200728
			(Litomosoides sigmodontis)

NL004	1454	AAGAACAAGGATATTCGTAAATTCTTGGA	21056283 (Ascaris suum), 2978237 (Toxocara canis)

NL004	1455	CCGTGTACGCCCATTTCCCCATCAAC	1783477 (Pristionchus pacificus)

NL004	1456	TACGCCCATTTCCCCATCAAC	2181209 (Haemonchus contortus)

NL007	1457	CAACATGAATGCATTCCTCAAGC	39747064 (Meloidogyne paranaensis)

NL007	1458	GAAGTACAACATGAATGCATTCC	6721002 (Onchocerca volvulus)

NL007	1459	GCTGTATTTGTGTTGGCGACA	27541378 (Meloidogyne hapla)

NL008	1460	AGAAAAGGTTGTGGGTTGGTA	108958003 (Bursaphelenchus mucronatus)

NL011	1461	GGACTTCGTGATGGATATTACATTCAGGGACAATG	33138488 (Meloidogyne incognita)

NL011	1462	CAACTACAACTTCGAGAAGCC	108984057 (Bursaphelenchus xylophilus)

NL014	1463	GAAGAATTCAACATTGAAAAGGG	11927908 (Haemonchus contortus)

NL014	1464	GAGCAAGAAGCCAATGAGAAAGC	108985855 (Bursaphelenchus mucronatus)

NL014	1465	TTTCATTGAGCAAGAAGCCAATGAGAAAGCCGAAGA	108979738 (Bursaphelenchus xylophilus)

NL015	1466	ATGAGCAAATTGGCCGGCGAGTCGGAG	18090737 (Globodera rostochiensis)

NL015	1467	CACACCAAGAACATGAAGTTGGCTGA	68276872 (Caenorhabditis remanei)

NL015	1468	CAGGAAATCTGTTCGAAGTGT	45564676 (Meloidogyne incognita)

NL015	1469	CTGGCGCAGATCAAAGAGATGGT	18090737 (Globodera rostochiensis)

NL015	1470	TGGCGCAGATCAAAGAGATGGT	27428872 (Heterodera glycines)

NL016	1471	TATCCCGAGGAAATGATCCAGAC	18081287 (Globodera rostochiensis)

NL016	1472	CGTATCTATCCCGAGGAAATGATCCAGACTGGAATTTC	108957716 (Bursaphelenchus mucronatus)
			108962248 (Bursaphelenchus xylophilus)

NL023	1473	TGGATGGGAGTCATGCATGGA	13959786 (Nippostrongylus brasiliensis)

TABLE 5-CS

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

CS001	1988	ATACAAGCTGTGCAAGGTGCG	10803803 (Trichuris muris)

CS003	1989	AAGCACATTGACTTCTCGCTGAA	18850138 (Ascaris suum)

CS003	1990	CGCAACAAGCGTGAGGTGTGG	40305701 (Heterodera glycines)

CS003	1991	CGTCTCCAGACTCAGGTGTTCAAG	91102965 (Nippostrongylus brasiliensis)

CS011	1992	TTTAATGTATGGGATACTGCTGG	9832495 (Strongyloides stercoralis)

CS011	1993	CACTTGACTGGAGAGTTCGAGAAAA	18082874 (Globodera rostochiensis)

CS011	1994	CTCGTGTCACCTACAAAAATGTACC	71182695 (Caenorhabditis remanei)

CS011	1995	CACTTGACTGGAGAGTTCGAGAA	108987391 (Bursaphelenchus xylophilus)

CS013	1996	TAGGTGAATTTGTTGATGATTA	40305096 (Heterodera glycines)

CS014	1997	AAGAAAGAGAAACAAGTGGAACT	51871231 (Xiphinema index)

CS016	1998	GTGTACATGTAACCTGGGAAACCACG	10819046 (Haemonchus contortus)

CS016	1999	GTGTACATGTAACCTGGGAAACC	13418283 (Necator americanus)

CS016	2000	GCCAAATCGGTGTACATGTAACC	54552787 (Xiphinema index)

CS016	2001	AAGTTCTTCTCGAACTTGGTGAGGAACTC	111163626 (Globodera rostochiensis)

TABLE 5-PX

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

PX001	2291	CTCGACATCGCCACCTGCAAG	11069004 (Haemonchus contortus); 27770634
			(Teladorsagia circumcincta)

PX001	2292	GACGGCAAGGTCCGCACCGAC	32320500 (Heterodera glycines)

PX001	2293	CCCGGCTGGATTCATGGATGT	51334233 (Radopholus similis)

PX001	2294	ATCAAGGTGGACGGCAAGGTCCGCAC	108959807 (Bursaphelenchus xylophilus)

PX001	2295	ACAACGTGTTCATCATCGGCAA	111166840 (Globodera rostochiensis)

PX016	2296	CGTGGTTTCCCAGGTTACATGTACACGGATTTGGC	10819046 (Haemonchus contortus)

PX016	2297	GGTTTCCCAGGTTACATGTACAC	13418283 (Necator americanus)

PX016	2298	GAGTTCCTCACCAAGTTCGAGAAGAACTT	111163626 (Globodera rostochiensis)

TABLE 5-AD

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

AD015	2439	ATAAATGGTCCTGAAATTATGA	9832193 (Strongyloides stercoralis)

AD016	2440	GTCAACATGGAGACGGCGCGCTT	30220804 (Heterodera glycines)

TABLE 6-LD

	SEQ
Target ID	ID No	Sequence*	Example Gi-number and species

LD001	136	TAGCGGATGGTGCGGCCGTCGTG	54625255 (Phlebiopsis gigantea)

LD003	137	TTCCAAGAAATCTTCAATCTTCAAA	50294437 (Candida glabrata CBS 138)

LD007	138	GACTGCGGTTTTGAACACCCTTCAGAAGTTCA	110463173 (Rhizopus oryzae)

LD007	139	TGTCAAGCCAAATCTGGTATGGG	110463173 (Rhizopus oryzae)

LD011	140	GGCTTCTCAAAGTTGTAGTTA	48898288 (Aspergillus flavus)

LD011	141	CCATCACGGAGACCACCAAACTT	60673229 (Alternaria brassicicola)

LD011	142	AAAGGCTTCTCAAAGTTGTAGTTA	58157923 (Phytophthora infestans)

LD011	143	TGTGCTATTATCATGTTTGATGT	110458937 (Rhizopus oryzae)

LD011	144	ACTGCCGGTCAGGAGAAGTTTGG	90638500 (Thermomyces lanuginosus)

LD011	145	AATACAACTTTGAGAAGCCTTTCCT	90549582 (Lentinula edodes), 90381505 (Amorphotheca resinae)

LD011	146	CAGGAGAAGTTTGGTGGTCTCCG	90544763 (Gloeophyllum trabeum)

LD011	147	ACCACCAAACTTCTCCTGACC	90368069 (Aureobasidium pullulans)

LD011	148	GGTCAGGAGAAGTTGGTGGTCTCCG	90355148 (Coprinopsis cenerea)

LD016	149	GCAGCAATTTCATTGTGAGGCAGACCAG	50285562 (Candida glabrata CBS 138)

LD016	150	ATGGAGTTCATCACGTCAATAGC	68419480 (Phytophthora parasitica)

LD016	151	GGTCTGCCTCACAATGAAATTGCTGCCCAGAT	85109950 (Neurospora crassa)

LD016	152	CTATTGTTTTCGCTGCTATGGGTGTTAACATG	50423336 (Debaryomyces hansenii), 90540142 (Gloeophyllum
		GA	trabeum)

LD016	153	ATGAACTCCATTGCTCGTGGTCAGAAGAT	84573655 (Aspergillus oryzae)

LD016	154	ATAGGAATCTGGGTGATGGATCCGTT	90562068 (Leucosporidium scottii), 90359845 (Aureobasidium
			pullulans)

LD016	155	TCCTGTTTCTGAAGATATGTTGGG	90388021 (Cunninghamella elegans)

LD016	156	TTTGAAGATTGAAGATTTCTTGGAACG	50294437 (Candida glabrata CBS 138), 110468393 (Rhizopus
			oryzae), 90388664 (Cunninghamella elegans), 90376235
			(Amorphotheca resinae)

LD027	157	TCACAGGCAGCGAAGATGGTACC	90546087 (Gloeophyllum trabeum)

LD027	158	TTCTTTGAAGTTTTTGAATAT	50292600 (Candida glabrata CBS 138)

TABLE 6-PC

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

PC001	447	CCCTGCTGGTTTCATGGATGTCAT	110469463 (Rhizopus oryzae)

PC003	448	ATTGAAGATTTCTTGGAAAGAAG	50294437 (Candida glabrata CBS 138)

PC003	449	TTGAAGATTTCTTGGAAAGAAG	50310014 (Kluyveromyces lactis NRRL Y-1140)

PC003	450	CTTCTTTCCAAGAAATCTTCAA	622611 (Saccharomyces cerevisiae)

PC003	451	GACTCGCAGAAGCACATCGACTT	109744873 (Allomyces macrogynus); 59284959
			(Blastocladiella emersonii); 90623359 (Corynascus
			heterothallicus); 29427071 (Verticillium dahliae)

PC003	452	GACTCGCAGAAGCACATCGACTTC	59298648 (Blastocladiella emersonii); 90565029
			(Leucosporidium scottii)

PC003	453	TCGCAGAAGCACATCGACTTC	47032157 (Mycosphaerella graminicola)

PC003	454	CAGAAGCACATCGACTTCTCCCT	34332427 (Ustilago maydis)

PC005	455	CTTATGGAGTACATCCACAAG	98997063 (Spizellomyces punctatus)

PC005	456	AAGAAGAAGCAGAGAAGGCCA	84572408 (Aspergillus oryzae)

PC010	457	GTGTTCAATAATTCTCCTGATGA	50288722 (Candida glabrata CBS 138)

PC010	458	ATTTTCCATGGAGAGACCATTGC	70990481 (Aspergillus fumigatus)

PC010	459	GGGCAGAATCCCCAAGCTGCC	90631635 (Thermomyces lanuginosus)

PC014	460	AATACAAGGACGCCACCGGCA	30394561 (Magnaporthe grisea)

PC016	461	ATGCCCAACGACGACATCACCCA	59281308 (Blastocladiella emersonii)

PC016	462	TGGGTGATGTCGTCGTTGGGCAT	38353161 (Hypocrea jecorina)

PC016	463	GGTTTCCCCGGTTACATGTACAC	34447668 (Cryphonectria parasitica)

PC016	464	ACTATGCCCAACGACGACATCAC	34447668 (Cryphonectria parasitica)

PC016	465	CCGGGCACTTCTTCTCGAGCGGC	38353161 (Hypocrea jecorina)

PC016	466	CCGACCATCGAGCGCATCATCAC	59281308 (Blastocladiella emersonii)

PC016	467	TTCTTGAACTTGGCCAACGATCC	50285562 (Candida glabrata CBS 138)

PC016	468	TGTTCTTGAACTTGGCCAACGA	66909391 (Phaeosphaeria nodorum)

PC016	469	GCTATGGGTGTCAACATGGAAACTGC	110463410 (Rhizopus oryzae)

PC016	470	TGCTATGGGTGTCAACATGGA	71006197 (Ustilago maydis)

PC016	471	CTATTGTGTTTGCTGCTATGGGTGT	68488910 (Candida albicans)

PC016	472	TACGAGCGCGCCGGTCGTGTGGA	90347883 (Coprinopsis cinerea)

TABLE 6-EV

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

EV010	565	TTCAATAATTCACCAGATGAAAC	50405834 (Debaryomyces hansenii)

EV015	566	CGATCGCCTTGAACAGCGACG	22502898 (Gibberella zeae)

EV015	567	GTTACCATGGAGAACTTCCGTTA	67900533 (Aspergillus nidulans FGSC A4)

EV015	568	GTTACCATGGAGAACTTCCGTTACGCC	70820241 (Aspergillus niger)

EV015	569	ACCATGGAGAACTTCCGTTACGCC	84573628 (Aspergillus oryzae)

EV015	570	ATGGAGAACTTCCGTTACGCC	71002727 (Aspergillus fumigatus)

EV016	571	TCTGAAGATATGTTGGGTCGTGT	90396765 (Cunninghamella elegans)

EV016	572	CAAAAGATTCCAATTTTCTCTGCA	50306984 (Kluyveromyces lactis NRRL Y-1140)

EV016	573	CCCCACAATGAAATCGCTGCTCAAAT	68001221 (Schizosaccharomyces pombe 972h-)

EV016	574	ATCGTTTTCGCCGCTATGGGTGT	58271359 (Cryptococcus neoformans var.)

EV016	575	TTCAAGCAAGATTTTGAAGAGAATGG	50285562 (Candida glabrata CBS 138)

TABLE 6-AG

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

AG001	752	CGTAACAGGTTGAAGTACGCCCT	16931515 (Coccidioides posadasii)

AG001	753	AAGGTCGACGGCAAAGTCAGGACTGAT	33515688 (Cryptococcus neoformans var.)

AG001	754	CCATTCTTGGTCACCCACGATG	38132640 (Hypocrea jecorina)

AG001	755	ATCAAGGTAAACGACACCATC	56939474 (Puccinia graminis f. sp.)

AG005	756	TGTACATGAAGGCCAAGGGTAACGTGTTCAAGAACAAG	98997063 (Spizellomyces punctatus)

AG005	757	CCAAGGGTAACGTGTTCAAGAACAAG	109744763 (Allomyces macrogynus);
			59297176 (Blastocladiella emersonii)

AG005	758	AAGGGTAACGTGTTCAAGAACAAG	109741162 (Allomyces macrogynus)

AG005	759	CAAGAAGAAGGCTGAGAAGGC	67903433 (Aspergillus nidulans FGSC A4)

AG005	760	CAAGAAGAAGGCTGAGAAGGC	4191107 (Emericella nidulans)

AG005	761	AAGAAGAAGGCTGAGAAGGCC	66909252 (Phaeosphaeria nodorum)

AG005	762	CAAAACATCCGTAAATTGATCAAGGATGGTTT	21649803 (Conidiobolus coronatus)

AG016	763	TTCGCCGCCATGGGTGTCAAC	50554108 (Yarrowia lipolytica)

AG016	764	ATGGGTGTCAACATGGAAACCGC	90639144 (Trametes versicolor)

AG016	765	TGGAAACCGCCCGTTTCTTCA	85109950 (Neurospora crassa)

AG016	766	GGTTACATGTACACCGATTTG	32169825 (Mucor circinelloides)

AG016	767	GTCAAGATGGGAATCTGGGTGATGGA	38353161 (Hypocrea jecorina)

TABLE 6-TC

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

TC001	855	AACAGGCTGAAGTATGCCTTGACC	90545567 (Gloeophyllum trabeum)

TC015	856	TTCATCGTCCGTGGTGGCATG	46122304 (Gibberella zeae PH-1)

TC015	857	AGTTTTACCGGTACCTGGAGG	50310636 (Kluyveromyces lactis NRRL Y-1140)

TC015	858	CCTCCAGGTACCGGTAAAACT	85114224 (Neurospora crassa)

TC015	859	CCTCCAGGTACCGGTAAAACTTT	50290674 (Candida glabrata CBS 138)

TC015	860	ATTAAAGTTTTACCGGTACCTGGAGG	3356460 (Schizosaccharomyces pombe)

TC015	861	GGTGCTTTCTTCTTCTTAATCAA	21649889 (Conidiobolus coronatus)

TC015	862	ATCAACGGTCCCGAAATTATG	82610024 (Phanerochaete chrysosporium)

TABLE 6-MP

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

MP002	1026	AATTTTTAGAAAAAAAAATTG	68026454 (Schizosaccharomyces pombe 972h-)

MP010	1027	GTCACCACATTAGCTAGGAAT	48564349 (Coccidioides posadasii)

MP016	1028	AAGAAATGATTCAAACTGGTAT	90396765 (Cunninghamella elegans)

MP016	1029	AAGAAATGATTCAAACTGGTATTTC	110463410 (Rhizopus oryzae)

MP016	1030	CATGAACTCTATTGCTCGTGG	50285562 (Candida glabrata CBS 138)

MP016	1031	GCTGCTATGGGTGTTAATATGGA	90348219 (Coprinopsis cinerea)

MP016	1032	TGCTATGGGTGTTAATATGGAAAC	90396964 (Cunninghamella elegans)

MP016	1033	CCTACTATTGAGCGTATCATTAC	90524974 (Geomyces pannorum)

MP016	1034	GAAGTTTCTGCTGCTCGTGAAGAAGTACCTGG	90396313 (Cunninghamella elegans)

MP016	1035	GTTTCTGCTGCTCGTGAAGAAGT	32169825 (Mucor circinelloides)

MP016	1036	GTGTACATGTAACCAGGGAAACCACG	45392344 (Magnaporthe grisea)

MP016	1037	CCTGGTTACATGTACACCGATTT	32169825 (Mucor circinelloides)

MP016	1038	GGTTACATGTACACCGATTTA	47067814 (Eremothecium gossypii)

MP016	1039	CCTATTTTAACTATGCCTAACGA	90396313 (Cunninghamella elegans)

MP027	1040	ACTCTCCATCACCACATACTA	60673889 (Alternaria brassicicola)

TABLE 6-NL

	SEQ
Target ID	ID No	Sequence*	Example Gi-number and species

NL001	1474	CCAAGGGCAAGGGTGTGAAGCTCA	30418788 (Magnaporthe grisea)

NL001	1475	TCTCTGCCCAAGGGCAAGGGTGT	22500578 (Gibberella zeae), 46128672 (Gibberella zeae PH-1),
			70662858 (Gibberella moniliformis), 71000466 (Aspergillus
			fumigatus)

NL001	1476	TCTGCCCAAGGGCAAGGGTGT	14664568 (Fusarium sporotrichioides)

NL001	1477	TCTCTGCCCAAGGGCAAGGGT	50550586 (Yarrowia lipolytica)

NL001	1478	TCTCTGCCCAAGGGCAAGGGTGT	71000466 (Aspergillus fumigatus)
			92459259 (Gibberella zeae)

NL001	1479	CTGCCCAAGGGCAAGGGTGTGAAG	90545567 (Gloeophyllum trabeum)

NL003	1480	ATGAAGCTCGATTACGTCTTGG	24446027 (Paracoccidioides brasiliensis)

NL003	1481	CGTAAGGCCGCTCGTGAGCTG	10229753 (Phytophthora infestans)

NL003	1482	CGTAAGGCCGCTCGTGAGCTGTTGAC	58082846 (Phytophthora infestans)

NL003	1483	GACTCGCAGAAGCACATTGACTT	21393181 (Pratylenchus penetrans), 34330401 (Ustilago
			maydis)

NL003	1484	TGAAGCTCGATTACGTCTTGG	46346864 (Paracoccidioides brasiliensis)

NL003	1485	TGGCCAAGTCCATCCATCACGCCCGTGT	58113938 (Phytophthora infestans)

NL004	1486	CGTAACTTCCTGGGCGAGAAG	58127885 (Phytophthora infestans)

NL003	1487	ATGAAGCTCGATTACGTCTTGG	90366381 (Aureobasidium pullulans)

NL003	1488	TCGGTTTGGCCAAGTCCATCCA	90353540 (Coprinopsis cinerea)

NL003	1489	GACTCGCAGAAGCACATTGACTT	71012467 (Ustilago maydis)

NL003	1490	GACTCGCAGAAGCACATTGACTTCTC	90616286 (Ophiostoma piliferum)

NL004	1491	TACGCCCATTTCCCCATCAAC	15771856 (Gibberella zeae), 29426217 (Verticillium dahliae),
			30399988 (Magnaporthe grisea), 34330394 (Ustilago maydis),
			39945691 (Magnaporthe grisea 70-15), 46108543 (Gibberella
			zeae PH-1), 70660620 (Gibberella moniliformis)

NL004	1492	CGTGTACGCCCATTTCCCCATCAAC	90615722 (Ophiostoma piliferum)

NL004	1493	TACGCCCATTTCCCCATCAAC	90367524 (Aureobasidium pullulans)
			90372622 (Cryptococcus laurentii)
			109654277 (Fusarium oxysporum f. sp.)
			90535059 (Geomyces pannorum)
			46108543 (Gibberella zeae PH-1)
			90566138 (Leucosporidium scottii)
			39945691 (Magnaporthe grisea 70-15)
			110115733 (Saitoella complicata)
			110081735 (Tuber borchii)
			71021510 (Ustilago maydis)
			50554252 (Yarrowia lipolytica)

NL004	1494	TACGCCCATTTCCCCATCAACTG	90640952 (Trametes versicolor)

NL004	1495	CGTGTACGCCCATTTCCCCATCAAC	90615722 (Ophiostoma piliferum)

NL005	1496	AAAAGGTCAAGGAGGCCAAGA	14662414 (Fusarium sporotrichioides)

NL005	1497	TTCAAGAACAAGCGTGTATTGATGGA	90395504 (Cunninghamella elegans)

NL005	1498	TTCAAGAACAAGCGTGTATTGATGGAGT	90542553 (Gloeophyllum trabeum)

NL006	1499	CCTGGAGGAGGAGACGACCAT	70998503 (Aspergillus fumigatus)

NL006	1500	TCCCATCTCGTATGACAATTGG	68471154 (Candida albicans)

NL006	1501	ATGGTCGTCTCCTCCTCCAGG	70998503 (Aspergillus fumigatus)

NL006	1502	TCCCATCTCGTATGACAATTGG	68471154 (Candida albicans)
			50425488 (Debaryomyces hansenii)

NL007	1503	CAAGTCATGATGTTCAGTGCAAC	70984614 (Aspergillus fumigatus)

NL007	1504	TGACGCTTCACGGCCTGCAGCAG	10229203 (Phytophthora infestans)

NL007	1505	CAAGTCATGATGTTCAGTGCAAC	70984614 (Aspergillus fumigatus)

NL010_2	1506	CAATTCTTGCAAGTGTTCAACAA	68478799 (Candida albicans)

NL010_2	1507	TTCAACAACAGTCCTGATGAAAC	21649260 (Conidiobolus coronatus)

NL010_2	1508	TTCTTGCAAGTGTTCAACAAC	47031965 (Mycosphaerella graminicola)

NL011	1509	AAGAACGTTCCCAACTGGCAC	68132303 (Trichophyton rubrum)

NL011	1510	ACAAGAACGTTCCCAACTGGCA	68132303 (Trichophyton rubrum)

NL011	1511	ACCTACAAGAACGTTCCCAACT	68132303 (Trichophyton rubrum)

NL011	1512	ACCTACAAGAACGTTCCCAACTGGCAC	70674996 (Gibberella moniliformis)

NL011	1513	CAACTACAACTTCGAGAAGCC	22500425 (Gibberella zeae), 34331122 (Ustilago maydis),
			46108433 (Gibberella zeae PH-1), 47029512 (Mycosphaerella
			graminicola), 56236507 (Setosphaeria turcica), 62926335
			(Fusarium oxysporum f. sp.), 70674996 (Gibberella
			moniliformis), 70992714 (Aspergillus fumigatus)

NL011	1514	CAAGAACGTTCCCAACTGGCAC	68132303 (Trichophyton rubrum)

NL011	1515	CACCTACAAGAACGTTCCCAAC	68132303 (Trichophyton rubrum)

NL011	1516	CCTACAAGAACGTTCCCAACTG	68132303 (Trichophyton rubrum)

NL011	1517	CTACAAGAACGTTCCCAACTGG	68132303 (Trichophyton rubrum)

NL011	1518	GCAACTACAACTTCGAGAAGCC	22505588 (Gibberella zeae)

NL011	1519	TACAAGAACGTTCCCAACTGGC	68132303 (Trichophyton rubrum)

NL011	1520	TCACCTACAAGAACGTTCCCA	68132303 (Trichophyton rubrum)

NL011	1521	TCACCTACAAGAACGTTCCCAA	68132303 (Trichophyton rubrum)

NL011	1522	TCACCTACAAGAACGTTCCCAACT	30405871 (Magnaporthe grisea)

NL011	1523	TCACCTACAAGAACGTTCCCAACTGGCAC	13903501 (Blumeria graminis f. sp.), 3140444 (Emericella
			nidulans), 34331122 (Ustilago maydis), 49096317 (Aspergillus
			nidulans FGSC A4)

NL011	1524	TGGGACACAGCTGGCCAGGAAA	14180743 (Magnaporthe grisea), 39950145 (Magnaporthe
			grisea 70-15)

NL011	1525	TTCGAGAAGCCGTTCCTGTGG	38056576 (Phytophthora sojae), 45244260 (Phytophthora
			nicotianae), 58091236 (Phytophthora infestans)

NL011	1526	TTCGAGAAGCCGTTCCTGTGGTTGGC	58090083 (Phytophthora infestans)

NL011	1527	TGGGACACAGCTGGCCAGGAAA	39950145 (Magnaporthe grisea 70-15)

NL011	1528	TATTACATTCAGGGACAATGCG	110134999 (Taphrina deformans)

NL011	1529	TCACCTACAAGAACGTTCCCAACTGGCAC	84573903 (Aspergillus oryzae)
			90355199 (Coprinopsis cinerea)
			90624693 (Corynascus heterothallicus)
			90638500 (Thermomyces lanuginosus)

NL011	1530	ACCTACAAGAACGTTCCCAACTGGCAC	113544700 (Cordyceps bassiana)
			85114463 (Neurospora crassa)

NL011	1531	TACAAGAACGTTCCCAACTGGCA	110269748 (Hypocrea lixii)

NL011	1532	TACAAGAACGTTCCCAACTGGCAC	110458937 (Rhizopus oryzae)

NL011	1533	AGGAAGAAGAACCTTCAGTACT	90557551 (Leucosporidium scottii)

NL011	1534	AAGAAGAACCTTCAGTACTACGA	113551594 (Cordyceps bassiana)

NL011	1535	AAGAAGAACCTTCAGTACTACGACATC	90036917 (Trichophyton rubrum)

NL011	1536	AAGAACCTTCAGTACTACGACATC	90624693 (Corynascus heterothallicus)

NL011	1537	GGCTTCTCGAAGTTGTAGTTGC	89975123 (Hypocrea lixii)

NL011	1538	CAACTACAACTTCGAGAAGCC	70992714 (Aspergillus fumigatus)
			90368808 (Aureobasidium pullulans)
			90629512 (Corynascus heterothallicus)
			109656121 (Fusarium oxysporum f. sp.)
			90532849 (Geomyces pannorum)
			110272576 (Hypocrea lixii)
			47029512 (Mycosphaerella graminicola)
			85114463 (Neurospora crassa)
			90617165 (Ophiostoma piliferum)
			90036917 (Trichophyton rubrum)

NL011	1539	GGCTTCTCGAAGTTGTAGTTG	92233975 (Gibberella zeae)

NL013	1540	CCCGAGATGGTGGTGGGCTGGTACCA	49069733 (Ustilago maydis)

NL013	1541	GGTACCACTCGCACCCGGGCTT	58134950 (Phytophthora infestans)

NL013	1542	GTGGGCTGGTACCACTCGCACCCGGGCTTCGG	38062327 (Phytophthora sojae)
		CTGCTGGCTGTCGGG

NL013	1543	TGGTACCACTCGCACCCGGGCTT	58084933 (Phytophthora infestans)

NL013	1544	CCCGAGATGGTGGTGGGCTGGTACCA	71006043 (Ustilago maydis)

NL015	1545	ATCCACACCAAGAACATGAAG	10181857 (Aspergillus niger), 22505190 (Gibberella zeae),
			30394634 (Magnaporthe grisea), 33507832 (Cryptococcus
			neoformans var.), 3773467 (Emericella nidulans), 39940093
			(Magnaporthe grisea 70-15), 46122304 (Gibberella zeae PH-1),
			47032030 (Mycosphaerella graminicola), 49106059 (Aspergillus
			nidulans FGSC A4)

NL015	1546	CACACCAAGAACATGAAGTTGG	21649889 (Conidiobolus coronatus)

NL015	1547	GCCTTCTTCTTCCTCATCAACGG	46122304 (Gibberella zeae PH-1)

NL015	1548	TTGGAGGCTGCAGAAAGCAGCT	90369178 (Cryptococcus laurentii)

NL015	1549	GCCTTCTTCTTCCTCATCAACGG	46122304 (Gibberella zeae PH-1)

NL015	1550	ATCCACACCAAGAACATGAAG	70820941 (Aspergillus niger)
			58260307 (Cryptococcus neoformans var.)
			85691122 (Encephalitozoon cuniculi GB-M1)
			46122304 (Gibberella zeae PH-1)
			39940093 (Magnaporthe grisea 70-15)
			85082882 (Neurospora crassa)
			50555821 (Yarrowia lipolytica)

NL015	1551	CACACCAAGAACATGAAGTTGGC	110272618 (Hypocrea lixii)

NL016	1552	CATGAACTCGATTGCTCGTGG	30418452 (Magnaporthe grisea), 39942327 (Magnaporthe
			grisea 70-15)

NL016	1553	CCACCATCTACGAGCGCGCCGGACG	39942327 (Magnaporthe grisea 70-15), 45392344
			(Magnaporthe grisea)

NL016	1554	CATGAACTCGATTGCTCGTGG	90367610 (Aureobasidium pullulans)
			39942327 (Magnaporthe grisea 70-15)

NL016	1555	CATGTCGGTGAGGATGACGAG	90562068 (Leucosporidium scottii)

NL016	1556	CCACCATCTACGAGCGCGCCGGACG	39942327 (Magnaporthe grisea 70-15)

NL019	1557	CAGATTTGGGACACGGCCGGCCAGGAGCG	9834078 (Phytophthora sojae)

NL019	1558	GACCAGGAGTCGTTCAACAAC	9834078 (Phytophthora sojae)

NL019	1559	TGGGACACGGCCGGCCAGGAG	38056576 (Phytophthora sojae), 40545332 (Phytophthora
			nicotianae), 58083674 (Phytophthora infestans)

NL019	1560	TGGGACACGGCCGGCCAGGAGCG	29426828 (Verticillium dahliae), 38057141 (Phytophthora sojae)

NL019	1561	TGGGACACGGCCGGCCAGGAGCGGTT	70981934 (Aspergillus fumigatus)

NL019	1562	TTCCTGGAGACGTCGGCGAAGAACGC	90643518 (Trametes versicolor)

NL019	1563	CAGATTTGGGACACGGCCGGCCAGGAGCG	90616605 (Ophiostoma piliferum)

NL019	1564	TGGGACACGGCCGGCCAGGAG	110272626 (Hypocrea lixii)

NL019	1565	TGGGACACGGCCGGCCAGGAGCG	50550714 (Yarrowia lipolytica)

NL019	1566	TGGGACACGGCCGGCCAGGAGCGGTT	70981934 (Aspergillus fumigatus)

NL019	1567	TGGGACACGGCCGGCCAGGAGCGGTTCCG	50553761 (Yarrowia lipolytica)

NL022	1568	CAGGCAAAGATTTTCCTGCCCA	58124185 (Phytophthora infestans)

NL022	1569	GGCAAGTGCTTCCGTCTGTACAC	58124872 (Phytophthora infestans)

NL023	1570	GGATGACCAAAAACGTATTCT	46137132 (Gibberella zeae PH-1)

NL023	1571	AGAATACGTTTTTGGTCATCC	46137132 (Gibberella zeae PH-1)

TABLE 6-CS

	SEQ
Target ID	ID NO	Sequence*	Example Gi-number and species

CS003	2002	TGGTCTCCGCAACAAGCGTGA	46356829 (Paracoccidioides brasiliensis)

CS003	2003	GGTCTCCGCAACAAGCGTGAG	71012467 (Ustilago maydis)

CS003	2004	TGGTCTCCGCAACAAGCGTGAGGT	5832048 (Botryotinia fuckeliana)

CS003	2005	TGGTCTCCGCAACAAGCGTGAGGT	40545704 (Sclerotinia sclerotiorum)

CS003	2006	GGTCTCCGCAACAAGCGTGAGGT	21907821 (Colletotrichum trifolii); 90623359
			(Corynascus heterothallicus); 94331331
			(Pyronema omphalodes); 29427071 (Verticillium
			dahliae)

CS003	2007	TGGTCTCCGCAACAAGCGTGAGGTGTGG	27439041 (Chaetomium globosum); 47032270
			(Mycosphaerella graminicola)

CS003	2008	CGCAACAAGCGTGAGGTGTGG	71000428 (Aspergillus fumigatus); 67537265
			(Aspergillus nidulans FGSC A4); 70825441
			(Aspergillus niger); 84573806 (Aspergillus oryzae);
			3773212 (Emericella nidulans); 90632673
			(Thermomyces lanuginosus); 34332427 (Ustilago
			maydis)

CS006	2009	TCCCCTCTCGTATGACAATTGGT	68011927 (Schizosaccharomyces pombe 972h-)

CS007	2010	ATTTAGCTTTGACAAAGAATA	50305206 (Kluyveromyces lactis NRRL Y-1140)

CS007	2011	GAGCACCCTTCAGAAGTTCAACA	90553133 (Lentinula edodes)

CS011	2012	TGGGATACTGCTGGCCAAGAA	90385536 (Amorphotheca resinae); 68475609
			(Candida albicans); 50304104 (Kluyveromyces
			lactis NRRL Y-1140); 85105150 (Neurospora
			crassa)

CS011	2013	AAGTTTGGTGGTCTCCGAGATGGTTACTA	90355199 (Coprinopsis cinerea)

CS011	2014	CAATGTGCCATCATCATGTTCGA	15276938 (Glomus intraradices)

CS011	2015	CATCATCATGTTCGATGTAAC	28268268 (Chaetomium globosum)

CS011	2016	CACTTGACTGGAGAGTTCGAGAA	90368808 (Aureobasidium pullulans); 34331122
			(Ustilago maydis)

CS011	2017	TGAAGGTTCTTTTTTCTGTGGAA	6831345 (Pneumocystis carinii)

CS013	2018	GGATGGTACCACTCGCATCCTGG	109651225 (Fusarium oxysporum f. sp.)

CS015	2019	AACGAGAGGAAGAAGAAGAAG	39944615 (Magnaporthe grisea 70-15)

CS015	2020	AGGGCTTCTTCTTCTTCCTCTC	14662870 (Fusarium sporotrichioides)

CS015	2021	TAGGGCTTCTTCTTCTTCCTC	85112692 (Neurospora crassa)

CS015	2022	GAGATGGTCGAGTTGCCTCTA	71005073 (Ustilago maydis)

CS016	2023	GCTGAAGACTTTTTGGACATC	30418452 (Magnaporthe grisea)

CS016	2024	CCTCACCAAGTTCGAGAAGAACTTC	90566317 (Leucosporidium scottii)

CS016	2025	GTCGTCGGTGAGGAAGCCCTG	84573655 (Aspergillus oryzae)

CS016	2026	TCCTCACCGACGACAGCCTTCATGGCC	29427786 (Verticillium dahliae)

CS016	2027	GATGTTTCCAACCAGCTGTACGCC	90368806 (Aureobasidium pullulans)

CS016	2028	GGCGTACAGCTGGTTGGAAACATC	29427786 (Verticillium dahliae)

CS016	2029	TGATGTTTCCAACCAGCTGTACGCC	46107507 (Gibberella zeae PH-1)

CS016	2030	ATGGCAGACTTCATGAGACGAGA	29427786 (Verticillium dahliae)

CS016	2031	ATGCCCAACGACGACATCACCCA	59281308 (Blastocladiella emersonii)

CS016	2032	TGGGTGATGTCGTCGTTGGGCAT	38353161 (Hypocrea jecorina)

CS016	2033	ACTATGCCCAACGACGACATCAC	34447668 (Cryphonectria parasitica)

CS016	2034	GGTTACATGTACACCGATTTG	32169825 (Mucor circinelloides)

CS016	2035	CCCAGGTTACATGTACACCGATTT	47067814 (Eremothecium gossypii)

CS016	2036	ACACCACGTTTGGCCTTGACT	68488910 (Candida albicans)

CS016	2037	GCCATGGGTGTGAACATGGAGAC	82608508 (Phanerochaete chrysosporium)

CS016	2038	GACGACCACGAGGACAACTTTGCCATCGTGTTCG	59277641 (Blastocladiella emersonii)

CS016	2039	AAGATCCCCATTTTCTCGGCTGC	90348219 (Coprinopsis cinerea)

TABLE 6-PX

Target ID	SEQ ID NO	Sequence*	Example Gi-number and species

PX001	2299	CTCATCAAGGTGGACGGCAAGGT	85080580 (Neurospora crassa)

PX001	2300	TCGGTGCGGACCTTGCCGTCCACCTTGA	70768092 (Gibberella moniliformis)

PX001	2301	GACGGCAAGGTCCGCACCGAC	109745014 (Allomyces macrogynus); 60673542
			(Alternaria brassicicola); 90368699
			(Aureobasidium pullulans); 59299145
			(Blastocladiella emersonii); 27438899
			(Chaetomium globosum); 90623992 (Corynascus
			heterothallicus); 89975695 (Hypocrea lixii);
			99039195 (Leptosphaeria maculans); 39970560
			(Magnaporthe grisea); 47731115 (Metarhizium
			anisopliae); 90036859 (Trichophyton rubrum);
			29427127 (Verticillium dahliae)

PX001	2302	GACGGCAAGGTCCGCACCGACCC	70823112 (Aspergillus niger);
			90633197 (Thermomyces lanuginosus)

PX001	2303	AAGGTCCGCACCGACCCCACCTACCC	71015993 (Ustilago maydis)

PX001	2304	CGCTTCACCATCCACCGCATCAC	90639458 (Trametes versicolor)

PX001	2305	CGAGGAGGCCAAGTACAAGCTG	78177454 (Chaetomium cupreum);
			27438899 (Chaetomium globosum)

PX001	2306	GAGGCCAAGTACAAGCTGTGCAAGGT	109745014 (Allomyces macrogynus)

PX001	2307	GCCAAGTACAAGCTGTGCAAG	45923813 (Coccidioides posadasii)

PX001	2308	CCCGACCCGCTCATCAAGGTCAACGAC	78177454 (Chaetomium cupreum)

PX001	2309	CGACATCGTCCACATCAAGGAC	82603501 (Phanerochaete chrysosporium)

PX001	2310	CCGCACAAGCTGCGCGAGTGCCTGCCGCTC	109745014 (Allomyces macrogynus)

PX010	2311	TTCGACCAGGAGGCGGCGGCGGT	90542152 (Gloeophyllum trabeum)

PX010	2312	CACCACCGCCGCCGCCTCCTG	84578035 (Aspergillus oryzae)

PX010	2313	TGCAGGTCTTCAACAACTCGCCCGACGA	39978050 (Magnaporthe grisea)

PX010	2314	TTCAACAACTCGCCCGACGAGAC	90618424 (Corynascus heterothallicus)

PX015	2315	CATGCGCGCCGTCGAGTTCAAGGTGGT	59282860 (Blastocladiella emersonii)

PX015	2316	GCATTCTTCTTCCTCATCAACGG	68323226 (Coprinopsis cinerea)

PX015	2317	ATCAACGGCCCCGAGATCATGTC	85082882 (Neurospora crassa)

PX015	2318	TGCGCAAGGCGTTCGAGGAGGC	71002727 (Aspergillus fumigatus)

PX016	2319	CCTCACCAAGTTCGAGAAGAACTTC	90566317 (Leucosporidium scottii)

PX016	2320	GAGGAGATGATCCAGACTGGTAT	90639144 (Trametes versicolor)

PX016	2321	GAGGAGATGATCCAGACTGGTATCTC	58271359 (Cryptococcus neoformans)

PX016	2322	ATGAACTCCATCGCCCGTGGTCAGAAGATCCC	90545177 (Gloeophyllum trabeum)

PX016	2323	GTCAGAAGATCCCCATCTTCTCCGCC	9651842 (Emericella nidulans)

PX016	2324	CAGAAGATCCCCATCTTCTCCGC	70825597 (Aspergillus niger); 90611576
			(Ophiostoma piliferum); 90639144 (Trametes
			versicolor)

PX016	2325	CAGAAGATCCCCATCTTCTCCGCC	67540123 (Aspergillus nidulans)

PX016	2326	CAGAAGATCCCCATCTTCTCCGCCGCCGG	59283275 (Blastocladiella emersonii)

PX016	2327	AAGATCCCCATCTTCTCCGCCGCCGGTCT	34447668 (Cryphonectria parasitica)

PX016	2328	CCCATCTTCTCCGCCGCCGGTCTGCC	90621827 (Corynascus heterothallicus)

PX016	2329	GGTCTGCCCCACAACGAGATTGCTGC	90367610 (Aureobasidium pullulans);
			66909391 (Phaeosphaeria nodorum)

PX016	2330	TTCGCCGCCATGGGAGTCAACATGGAGAC	90562163 (Leucosporidium scottii)

PX016	2331	ACCGCCAGGTTCTTCAAGCAGGA	47067814 (Eremothecium gossypii)

PX016	2332	CTGTTCTTGAACTTGGCCAATGA	90545177 (Gloeophyllum trabeum)

PX016	2333	GGTTACATGTACACGGATTTG	34447668 (Cryphonectria parasitica); 90545177
			(Gloeophyllum trabeum); 39942327 (Magnaporthe
			grisea); 82608506 (Phanerochaete
			chrysosporium); 71006197 (Ustilago maydis)

PX016	2334	GGCAAGCCCATCGACAAGGGGCCC	59283275 (Blastocladiella emersonii)

PX016	2335	ATGGGGTGGGTGATGTCGTCGTTGGGCATGGTCA	38353161 (Hypocrea jecorina)

PX016	2336	ACCATGCCCAACGACGACATCACCCACCC	59281308 (Blastocladiella emersonii)

PX016	2337	TGCACAACAGGCAGATCTACCC	107889579 (Encephalitozoon cuniculi)

PX016	2338	CCGTCGCTATCTCGTCTCATGAA	48521040 (Coccidioides posadasii)

TABLE 6-AD

	SEQ
Target ID	ID NO	Sequence*	Example Gi-number and species

AD001	2441	CCCGCTGGTTTCATGGATGTT	58259586 (Cryptococcus neoformans)

AD001	2442	GACAACATCCATGAAACCAGCGGG	21649877 (Conidiobolus coronatus)

AD001	2443	TTCATGGATGTTGTCACCATTG	90616000 (Ophiostoma piliferum)

AD001	2444	GAAGAAGCCAAGTACAAGCTCTG	110469512 (Rhizopus oryzae)

AD001	2445	AAGAAGCCAAGTACAAGCTCTG	110469518 (Rhizopus oryzae)

AD001	2446	GCCAAGTACAAGCTCTGCAAGGT	98996590 (Spizellomyces punctatus)

AD001	2447	GCCAAGTACAAGCTCTGCAAGGTCA	109743129 (Allomyces macrogynus)

AD001	2448	AGTACAAGCTCTGCAAGGTCA	71000466 (Aspergillus fumigatus); 67537247
			(Aspergillus nidulans); 70823112 (Aspergillus niger);
			40886470 (Emericella nidulans)

AD015	2449	TATGGACCCCCTGGAACTGGTAAAACC	46349704 (Paracoccidioides brasiliensis)

AD016	2450	TGCCCGTGTCCGAGGACATGCTGGGCCG	109743322 (Allomyces macrogynus)

AD016	2451	TGCCCGTGTCCGAGGACATGCTGGGCCGC	59283275 (Blastocladiella emersonii)

AD016	2452	CGTGTCCGAGGACATGCTGGGCCGCA	90612905 (Ophiostoma piliferum)

AD016	2453	ATGGGCGTCAACATGGAGACGGC	59277641 (Blastocladiella emersonii)

AD016	2454	TGGAGACGGCGCGCTTCTTCA	90611376 (Ophiostoma piliferum)

AD016	2455	TTCCTCAACCTGGCCAACGACCCCAC	90611376 (Ophiostoma piliferum)

AD016	2456	ACCATCGAGCGCATCATCACCCCGCGCCTCGC	59281308 (Blastocladiella emersonii)

AD016	2457	TCCACCATCTACGAGCGCGCTGG	90368806 (Aureobasidium pullulans)

AD016	2458	CTGACGATGCCCAACGACGACATCAC	90611301 (Ophiostoma piliferum)

AD016	2459	ATGCCCAACGACGACATCACCCA	59281308 (Blastocladiella emersonii)

AD016	2460	TGGGTGATGTCGTCGTTGGGCAT	38353161 (Hypocrea jecorina)

TABLE 7-LD

Target ID	SEQ ID NO and DNA Sequence (sense strand) 5′ → 3′ of fragments and concatemer constructs

LD014_F1	SEQ ID NO: 159
	TCTAGAATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAA
	CGACTTGGTCAGGTCACAAACGCCCGGG

LD014_F2	SEQ ID NO: 160
	TCTAGAAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGCCCGGG

LD014_C1	SEQ ID NO: 161
	TCTAGAATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAA
	CGACTTGGTCAGGTCACAAACGATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACCGTACTA
	GAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCA
	CGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGCCCGGG

LD014_C2	SEQ ID NO: 162
	TCTAGAAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGAAGATCACGTTCGTACCGT
	ACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTT
	GGTCAGGTCACAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGAAGATCACG
	TTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGCCCGGG

TABLE 8-LD

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

LD001	SEQ ID NO: 164	SEQ ID NO: 165	SEQ ID NO: 163
	GCGTAATACGACTC	CCTTTGGGGCCAGT	GGCCCCAAGAAGCATTTGAAGCGTTTGAATGCCCCAAAAGCATGGATGTTGG
	ACTATAGGGGCCCC	TTGCATC	ATAAATTGGGAGGTGTTTTCGCACCTCGCCCATCTACAGGACCTCACAAATTG
	AAGAAGCATTTGAA	SEQ ID NO: 167	CGAGAGTCTTTGCCCTTGGTGATCTTCCTACGTAACCGATTGAAGTATGCTTT
	GCG	GCGTAATACGACTC	GACTAACAGCGAAGTTACTAAGATTGTTATGCAAAGGTTAATCAAAGTAGATG
	SEQ ID NO: 166	ACTATAGGCCTTTG	GAAAAGTGAGGACCGACTCCAATTACCCTGCTGGGTTTATGGATGTTATTACC
	GGCCCCAAGAAGCA	GGGCCAGTTTGCATC	ATTGAAAAAACTGGTGAATTTTTCCGACTCATCTATGATGTTAAAGGACGATTT
	TTTGAAGCG		GCAGTGCATCGTATTACTGCTGAGGAAGCAAAGTACAAACTATGCAAAGTCAG
			GAGGATGCAAACTGGCCCCAAAGG

LD002	SEQ ID NO: 169	SEQ ID NO: 170	SEQ ID NO: 168
	GCGTAATACGACTC	AAGCGATTAGAAAA	GTCCACGTCCAAGTTTTTATGGGCTTTCTTAAGAGCTTCAGCTGCATTTTTCAT
	ACTATAGGGTCCAC	AAATCAGTTGC	AGATTCCAATACTGTGGTGTTCGTACTAGCTCCCTCCAGAGCTTCTCGTTGAA
	GTCCAAGTTTTTATG	SEQ ID NO: 172	GTTCAATAGTAGTTAAAGTGCCATCTATTTGCAACTGATTTTTTTCTAATCGCTT
	GGC	GCGTAATACGACTC
	SEQ ID NO: 171	ACTATAGGAAGCGA
	GTCCACGTCCAAGT	TTAGAAAAAAATCAG
	TTTTATGGGC	TTGC

LD003	SEQ ID NO: 174	SEQ ID NO: 175	SEQ ID NO: 173
	GCGTAATACGACTC	GGTGACCACCACCG	GGTGACCACCACCGAATGGAGATTTGAGCGAGAAGTCAATATGCTTCTGGGA
	ACTATAGGCCCAGG	AATGGAG	ATCAAGTCTCACAATGAAGCTTGGAATATTCACGACCTGCTTACGAACCCTGA
	CGACCTTATGAAAA	SEQ ID NO: 177	TATGTCTTTGACGGACCAGCACACGAGCATGATGGATTGATTTTGCAAGCCCC
	GGC	GCGTAATACGACTC	AACTTGAAAACTTGTGTTTGGAGACGTCGTTCCAAGAAATCTTCAATCTTCAAA
	SEQ ID NO: 176	ACTATAGGGGTGAC	CCCAAGACGTAATCAAGCTTCATACGGGTTTCATCCAACACTCCAATACGCAC
	CCCAGGCGACCTTA	CACCACCGAATGGAG	CAACCGACGAAGAAGAGCATTGCCTTCAAACAACCTGCGCTGATCTTTCTCTT
	TGAAAAGGC		CCAAAGTCAGAAGTTCTCTGGCAGCTTTACGGATTTTTGCCAAGGTATACTTG
			ACTCGCCACACTTCACGTTTGTTCCTAAGACCATATTCTCCTATGATTTTCAAC
			TCCTGATCAAGACGTGCCTTTTCATAAGGTCGCCTGGG

LD006	SEQ ID NO: 179	SEQ ID NO: 180	SEQ ID NO: 178
	GCGTAATACGACTC	GCTTCGATTCGGCA	GGTGTTGGTTGCTTCTGGTGTGGTGGAATACATCGACACTCTTGAAGAAGAAA
	ACTATAGGGGTGTT	TCTTTATAGG	CTGTCATGATTGCGATGAATCCTGAGGATCTTCGGCAGGACAAAGAATATGCT
	GGTTGCTTCTGGTG	SEQ ID NO: 182	TATTGTACGACCTACACCCACTGCGAAATCCACCCGGCCATGATCTTGGGCG
	TG	GCGTAATACGACTC	TTTGCGCGTCTATTATACCTTTCCCCGATCATAACCAGAGCCCAAGGAACACC
	SEQ ID NO: 181	ACTATAGGGCTTCG	TACCAGAGCGCTATGGGTAAGCAAGCTATGGGGGTCTACATTACGAATTTCCA
	GGTGTTGGTTGCTT	ATTCGGCATCTTTAT	CGTGCGGATGGACACCCTGGCCCACGTGCTATACTACCCGCACAAACCTCTG
	CTGGTGTG	AGG	GTCACTACCAGGTCTATGGAGTATCTGCGGTTCAGAGAATTACCAGCCGGGA
			TCAACAGTATAGTTGCTATTGCTTGTTATACTGGTTATAATCAAGAAGATTCTG
			TTATTCTGAACGCGTCTGCTGTGGAAAGAGGATTTTTCCGATCCGTGTTTTAT
			CGTTCCTATAAAGATGCCGAATCGAAGC

LD007	SEQ ID NO: 184	SEQ ID NO: 185	SEQ ID NO: 183
	GCGTAATACGACTC	CCTTTCAATGTCCAT	GACTGGCGGTTTTGAACACCCTTCAGAAGTTCAGCACGAATGTATTCCTCAAG
	ACTATAGGGACTGG	GCCACG	CTGTCATTGGCATGGACATTTTATGTCAAGCCAAATCTGGTATGGGCAAAACG
	CGGTTTTGAACACCC	SEQ ID NO: 187	GCAGTGTTTGTTCTGGCGACACTGCAACAATTGGAACCAGCGGACAATGTTG
	SEQ ID NO: 186	GCGTAATACGACTC	TTTACGTTTTGGTGATGTGTCACACTCGTGAACTGGCTTTCCAAATCAGCAAA
	GACTGGCGGTTTTG	ACTATAGGCCTTTCA	GAGTACGAGAGGTTCAGTAAATATATGCCCAGTGTCAAGGTGGGCGTCTTTTT
	AACACCC	ATGTCCATGCCACG	CGGAGGAATGCCTATTGCTAACGATGAAGAAGTATTGAAAAACAAATGTCCAC
			ACATTGTTGTGGGGACGCCTGGGCGTATTTTGGCGCTTGTCAAGTCTAGGAA
			GCTAGTCCTCAAGAACCTGAAACACTTCATTCTTGATGAGTGCGATAAAATGT
			TAGAACTGTTGGATATGAGGAGAGACGTCCAGGAAATCTACAGAAACACCCC
			TCACACCAAGCAAGTGATGATGTTCAGTGCCACACTCAGCAAAGAAATCAGG
			CCGGTGTGCAAGAAATTCATGCAAGATCCAATGGAGGTGTATGTAGACGATG
			AAGCCAAATTGACGTTGCACGGATTACAACAGCATTACGTTAAACTCAAAGAA
			AATGAAAAGAATAAAAAATTATTTGAGTTGCTCGATGTTCTCGAATTTAATCAG
			GTGGTCATTTTTGTGAAGTCCGTTCAAAGGTGTGTGGCTTTGGCACAGTTGCT
			GACTGAACAGAATTTCCCAGCCATAGGAATTCACAGAGGAATGGACCAGAAA
			GAGAGGTTGTCTCGGTATGAGCAGTTCAAAGATTTCCAGAAGAGAATATTGGT
			AGCTACGAATCTCTTTGGGCGTGGCATGGACATTGAAAGG

LD010	SEQ ID NO: 189	SEQ ID NO: 190	SEQ ID NO: 188
	GCGTAATACGACTC	CTATCGGGTTGGAT	GCTTGTTGCCCCCGAATGCCTTGATAGGGTTGATTACCTTTGGGAAGATGGTC
	ACTATAGGGCTTGTT	GGAACTCG	CAAGTGCACGAACTAGGTACCGAGGGCTGCAGCAAATCTTACGTTTTCCGAG
	GCCCCCGAATGC	SEQ ID NO: 192	GGACGAAAGACCTCACAGCTAAGCAAGTTCAAGAGATGTTGGAAGTGGGCAG
	SEQ ID NO: 191	GCGTAATACGACTC	AGCCGCAGTAAGTGCTCAACCTGCTCCTCAACAACCAGGACAACCCATGAGG
	GCTTGTTGCCCCCG	ACTATAGGCTATCG	CCTGGAGCACTCCAGCAAGCTCCTACGCCACCAGGAAGCAGGTTCCTTCAAC
	AATGC	GGTTGGATGGAACT	CCATCTCGAAATGCGACATGAACCTCACTGATCTTATTGGAGAGTTGCAAAGA
		CG	GACCCATGGCCTGTCCACCAAGGCAAATGCGCCCTTAGATCGACCGGGACA
			GCTTTATCGATAGCCATTGGGTTGTTGGAGTGCACATACGCCAATACTGGTGC
			CAGGGTCATGCTATTCGTTGGAGGACCTTGCTCTCAAGGCCCTGGTCAAGTC
			TTGAATGATGATCTGAAGCAACCTATCAGATCTCACCACGACATCCAAAAAGA
			CAATGCCAAATACATGAAGAAAGCAATCAAGCACTATGATAATTTAGCGATGA
			GAGCAGCAACGAATGGCCACTGCGTTGACATATATTCATGCGCTTTGGATCA
			GACAGGATTGATGGAGATGAAACAGTGTTGTAATTCAACAGGGGGACATATG
			GTCATGGGCGACTCGTTCAATTCTTCCCTGTTCAAGCAAACGTTCCAGCGCAT
			ATTTTCGAAAGATCAGAAAAACGAGCTGAAGATGGCATTTAATGGTACTCTGG
			AGGGTCAAGTGTTCCAGGGAGTTGAAAATTCAAGGCGGTATTGGATCTTGTGT
			TTCGTTGAATGTGAAGAATCCTTTGGTTTCCGACACCGAAATAGGAATGGGTA
			ACACGGTCCAGTGGAAAATGTGTACGGTAACTCCAAGTACTACCATGGCCTT
			GTTCTTCGAGGTCGTCAACCAACATTCCGCTCCCATACCTCAAGGGGGAAGG
			GGCTGCATACAGTTCATCACGCAATATCAGCATGCTAGTGGCCAGAAGAGGA
			TCCGAGTAACGACAGTTGCTAGAAACTGGGCCGATGCTTCCGCTAATATACAT
			CATGTCAGTGCTGGATTCGATCAGGAGGCAGCCGCAGTGATAATGGCGAGGA
			TGGCAGTTTACAGAGCGGAATCAGACGATAGCCCTGATGTTTTGAGATGGGT
			CGATAGGATGTTGATACGTCTGTGCCAGAAATTCGGCGAATATAACAAGGAC
			GACCCGAATTCGTTCCGCTTGGGCGAAAACTTCAGCCTCTACCCGCAGTTCA
			TGTACCATTTGAGAAGGTCACAGTTCCTGCAGGTGTTTAACAATTCTCCCGAC
			GAAACGTCCTTCTACAGGCACATGCTTATGCGCGAAGACCTCACGCAGTCGC
			TGATCATGATCCAGCCGATACTCTACAGCTACAGTTTCAATGGACCACCAGAA
			CCTGTGCTTTTGGATACGAGTTCCATCCAACCCGATAG

LD011	SEQ ID NO: 194	SEQ ID NO: 195	SEQ ID NO: 193
	GCGTAATACGACTC	GGAAAAACGACATT	GCCATAGGAAAGGCTTCTCAAAGTTGTAGTTAGATTTGGCAGAGATATCATAG
	ACTATAGGGCCATA	TGTGAAACGTC	TACTGCAAATTCTTCTTCCTATGAAAGACAATACTTTTCGCTTTTACTTTTCTGT
	GGAAAGGCTTCTCA	SEQ ID NO: 197	CTTTGATGTCAACCTTGTTCCCGCAAAGTACTATCGGGATATTTTCACAGACTC
	AAG	GCGTAATACGACTC	TGACAAGATCTCTGTGCCAATTTGGTACATTCTTGTATGTAACTCTGGAAGTTA
	SEQ ID NO: 196	ACTATAGGGGAAAA	CATCAAACATGATAATAGCACACTGTCCCTGAATGTAATATCCATCACGGAGA
	GCCATAGGAAAGGC	ACGACATTTGTGAAA	CCACCAAACTTCTCCTGACCGGCAGTGTCCCATACATTGAACCGAATAGGGC
	TTCTCAAAG	CGTC	CCCTGTTTGTATGGAAGACCAGAGGATGGACTTCAACTCCCAAAGTAGCTACA
			TATCTTTTTTCAAATTCACCAGTCATATGACGTTTCACAAATGTCGTTTTTCC

LD014	SEQ ID NO: 199	SEQ ID NO: 200	SEQ ID NO: 198
	GCGTAATACGACTC	GCGAAATCAGCTCC	TTTCATTGAACAAGAGGCAAACGAAAAGGCAGAAGAAATCGATGCCAAGGCC
	ACTATAGGTTTCATT	AGACGAGC	GAGGAAGAATTTAATATTGAAAAGGGGCGCCTTGTTCAGCAACAACGTCTCAA
	GAACAAGAGGCAAA	SEQ ID NO: 202	GATTATGGAATATTATGAGAAGAAAGAGAAACAGGTCGAACTCCAGAAAAAAA
	CG	GCGTAATACGACTC	TCCAATCGTCTAACATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGG
	SEQ ID NO: 201	ACTATAGGGCGAAA	GAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGG
	TTTCATTGAACAAGA	TCAGCTCCAGACGA	TCACAAACGACCAGGGAAAATATTCCCAAATCCTGGAAAGCCTCATTTTGCAG
	GGCAAACG	GC	GGATTATATCAGCTTTTTGAGAAAGATGTTACCATTCGAGTTCGGCCCCAGGA
			CCGAGAACTGGTCAAATCCATCATTCCCACCGTCACGAACAAGTATAAAGATG
			CCACCGGTAAGGACATCCATCTGAAAATTGATGACGAAATCCATCTGTCCCAA
			GAAACCACCGGGGGAATCGACCTGCTGGCGCAGAAAAACAAAATCAAGATCA
			GCAATACTATGGAGGCTCGTCTGGAGCTGATTTCGC

LD014_F1	SEQ ID NO: 204	SEQ ID NO: 205	SEQ ID NO: 203
	GCGTAATACGACTC	CGTTTGTGACCTGA	ATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCG
	ACTATAGGATGTTGA	CCAAGTC	TACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACG
	ATCAGGCTCGATTG	SEQ ID NO: 207
	SEQ ID NO: 206	GCGTAATACGACTC
	ATGTTGAATCAGGC	ACTATAGGCGTTTGT
	TCGATTG	GACCTGACCAAGTC

LD014_F2	SEQ ID NO: 209	SEQ ID NO: 210	SEQ ID NO: 208
	GCGTAATACGACTC	CGTTTGTGACCTGA	AAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGT
	ACTATAGGAAGATC	CCAAG	CACAAACG
	ACGTTCGTACCGTAC	SEQ ID NO: 212
	SEQ ID NO: 211	GCGTAATACGACTC
	AAGATCACGTTCGT	ACTATAGGCGTTTGT
	ACCGTAC	GACCTGACCAAG

LD014_C1			SEQ ID NO: 213
			AATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTC
			GTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGATGT
			TGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACC
			GTACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGATGTTGAAT
			CAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACCGTACT
			AGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGC

LD014_C2			SEQ ID NO: 214
			AAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACTTGGTCAGG
			TCACAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGTAAACGACT
			TGGTCAGGTCACAAACGAAGATCACGTTCGTACCGTACTAGAGGAGGCGCGT
			AAACGACTTGGTCAGGTCACAAACGAAGATCACGTTCGTACCGTACTAGAGG
			AGGCGCGTAAACGACTTGGTCAGGTCACAAACGAAGATCACGTTCGTACCGT
			ACTAGAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGC

LD015	SEQ ID NO: 216	SEQ ID NO: 217	SEQ ID NO: 215
	GCGTAATACGACTC	CTATCGGCGTGAAG	CGCCGGAGAGTTTTTGTCAGCTTCTTCAAAAGCTTTGCGCAAGTTACTCTCAG
	ACTATAGGCGCCGG	CCCCC	ACTCGCCAGCGAGTTTGCTCATGATCTCCGGCCCGTTTATCAAGAAGAAGAA
	AGAGTTTTTGTCAGC	SEQ ID NO: 219	CGCCCCAGTCTCATTAGCCACGGCGCGAGCAATCAGGGTCTTACCCGTACCA
	SEQ ID NO: 218	GCGTAATACGACTC	GGGGGACCATACAGCAGTATACCCCTAGGGGGCTTCACGCCGATAG
	CGCCGGAGAGTTTT	ACTATAGGCTATCG
	TGTCAGC	GCGTGAAGCCCCC

LD016	SEQ ID NO: 221	SEQ ID NO: 222	SEQ ID NO: 220
	GCGTAATACGACTC	GGTAATCCTCGAAG	GGCATAGTCAATATAGGAATCTGGGTGATGGATCCGTTACGTCCTTCAACACG
	ACTATAGGGGCATA	ATGTTAAGTTCC	GCCGGCACGTTCATAGATGGTAGCTAAATCGGTGTACATGTAACCTGGGAAA
	GTCAATATAGGAATC	SEQ ID NO: 224	CCACGACGACCAGGCACCTCTTCTCTGGCAGCAGATACCTCACGCAAAGCTT
	TGGGTG	GCGTAATACGACTC	CTGCATACGAAGACATATCTGTCAAGATGACCAAGACGTGCTTCTCACATTGG
	SEQ ID NO: 223	ACTATAGGGGTAAT	TAAGCCAAGAATTCGGCAGCTGTCAAAGCCAGACGAGGTGTAATAATTCTTTC
	GGCATAGTCAATATA	CCTCGAAGATGTTA	AATGGTAGGATCGTTGGCCAAATTCAAGAACAGGCAGACATTCTCCATAGAAC
	GGAATCTGGGTG	AGTTCC	CGTTCTCTTCGAAATCCTGTTTGAAGAACCTAGCTGTTTCCATGTTAACACCCA
			TAGCAGCGAAAACAATAGCAAAGTTATCTTCATGATCATCAAGTACAGATTTAC
			CAGGAATCTTGACTAAACCAGCCTGTCTACAGATCTGGGCAGCAATTTCATTG
			TGAGGCAGACCAGCTGCAGAGAAAATGGGGATCTTCTGACCACGAGCAATGG
			AGTTCATCACGTCAATAGCTGTAATACCCGTCTGGATCATTTCCTCAGGATAG
			ATACGGGACCACGGATTGATTGGTTGACCCTGGATGTCCAAGAAGTCTTCAG
			CCAAAATTGGGGGACCTTTGTCGATGGGTTTTCCTGATCCATTGAAAACACGT
			CCCAACATATCTTCAGAAACAGGAGTCCTCAAAATATCTCCTGTGAATTCACAA
			GCGGTGTTTTTGGCGTCGATTCCTGATGTGCCCTCGAACACTTGAACCACAG
			CTTTTGACCCACTGACTTCCAGAACTTGTCCCGAACGTATAGTGCCATCAGCC
			AGTTTGAGTTGTACGATTTCATTGTACTTGGGGAACTTAACATCTTCGAGGATT
			ACC

LD018	SEQ ID NO: 226	SEQ ID NO: 227	SEQ ID NO: 225
	GCGTAATACGACTC	GTAGAGGCTCCACC	GGAGTCGCAGAAATACGAGAGCACCTTCTCGAACAACCAAGCCTCCTTGAGG
	ACTATAGGGGAGTC	GTCAATCGC	GTAAAACAAGCCCAGTCTGAGGACTCGGGACACTACACTTTGTTGGCGGAGA
	GCAGAAATACGAGA	SEQ ID NO: 229	ACCCTCAAGGCTGCATAGTGTCATCTGCTTACTTAGCCATAGAACCGGTAACC
	GCAC	GCGTAATACGACTC	ACCCAGGAAGGGTTGATCCACGAGTCCACCTTCAAGCAGCAACAGACCGAAA
	SEQ ID NO: 228	ACTATAGGCTACAG	TGGAGCAAATCGACACCAGCAAGACCTTGGCGCCTAACTTCGTCAGGGTTTG
	GGAGTCGCAGAAAT	GCTCCACCGTCAAT	CGGGGATAGAGACGTGACCGAGGGCAAGATGACCCGCTTCGACTGTCGCGT
	ACCACACCAC	CGC	CACTGGTCGTCCTTATCCAGACGTGACATGGTACATAAACGGTCGACAAGTCA
			CCGACGACCACAACCACAAGATTTTGGTTAACGAATCCGGAAACCATGCCCT
			GATGATCACCACCGTGAGCAGGAACGACTCAGGAGTAGTGACCTGCGTCGC
			CAGGAACAAGACGGGAGAAACCTCCTTCCAGTGCAACCTTAACGTCATCGAA
			AAGGAACAGGTAGTCGCGCCCAAGTTCGTGGAGAGATTTACCACAGTCAACG
			TGGCAGAAGGAGAACCAGTGTCTCTGCGCGCTAGAGCTGTTGGCACGCCGG
			TGCCGCGAATCACTTGGCAGAGGGACGGGGCGCCCCTAGCCAGCGGGCCC
			GACGTTCGCATCGCGATTGACGGTGGAGCCTCTAC

LD027	SEQ ID NO: 231	SEQ ID NO: 232	SEQ ID NO: 230
	GCGTAATACGACTC	TCGGACAGACTCGT	GGGAGCAGACGATCGGTTGGTTAAAATCTGGGACTATCAAAACAAAACGTGT
	ACTATAGGGGGAGC	TCATTTCCC	GTCCAAACCTTGGAAGGACACGCCCAAAACGTAACCGCGGTTTGTTTCCACC
	AGACGATCGGTTGG	SEQ ID NO: 234	CTGAACTACCTGTGGCTCTCACAGGCAGCGAAGATGGTACCGTTAGAGTTTG
	SEQ ID NO: 233	GCGTAATACGACTC	GCATACGAATACACACAGATTAGAGAATTGTTTGAATTATGGGTTCGAGAGAG
	GGGAGCAGACGATC	ACTATAGGTCGGAC	TGTGGACCATTTGTTGCTTGAAGGGTTCGAATAATGTTTCTCTGGGGTATGAC
	GGTTCG	AGACTCGTTCATTTC	GAGGGCAGTATATTAGTGAAAGTTGGAAGAGAAGAACCGGCAGTTAGTATGG
		CC	ATGCCAGTGGCGGTAAAATAATTTGGGCAAGGCACTCGGAATTACAACAAGC
			TAATTTGAAGGCGCTGCCAGAAGGTGGAGAAATAAGAGATGGGGAGCGTTTA
			CCTGTCTCTGTAAAAGATATGGGAGCATGTGAAATATACCCTCAAACAATCCA
			ACATAATCCGAATGGAAGATTCGTTGTAGTATGCGGAGACGGCGAATATATCA
			TTTACACAGCGATGGCTCTACGGAACAAGGCTTTTGGAAGCGCTCAAGAGTTT
			GTCTGGGCTCAGGACTCCAGCGAGTATGCCATTCGCGAGTCTGGTTCCACAA
			TTCGGATATTCAAAAACTTCAAAGAAAGGAAGAACTTCAAGTCGGATTTCAGC
			GCGGAAGGAATCTACGGGGGTTTTCTCTTGGGGATTAAATCGGTGTCCGGTT
			TAACGTTTTACGATTGGGAAACTTTGGACTTGGTGAGACGGATTGAAATACAA
			CCGAGGGCGGTTTATTGGTCTGACAGTGGAAAATTAGTCTGTCTCGCAACGG
			AGGACAGCTACTTCATCCTTTCTTATGATTCGGAGCAAGTTCAGAAGGCCAGG
			GAGAACAATCAAGTCGCAGAGGATGGCGTAGAGGCCGCTTTCGATGTGTTGG
			GGGAAATGAACGAGTCTGTCCGA

gfp	SEQ ID NO: 236	SEQ ID NO: 237	SEQ ID NO: 235
	GCGTAATACGACTC	CAATTTGTGTCCAAG	AGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGA
	ACTATAGGAGATAC	AATGTTTCC	AGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGA
	CCAGATCATATGAAA	SEQ ID NO: 239	CACGTAAGTTTAAACAGTTCGGTACTAACTAACCATACATATTTAAATTTTCAG
	CGG	GCGTAATACGACTC	GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAA
	SEQ ID NO: 238	ACTATAGGCAATTTG	GGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTG
	AGATACCCAGATCA	TGTCCAAGAATGTTT
	TATGAAACGG	CC

TABLE 8-PC

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

PC001	SEQ ID NO: 474	SEQ ID NO: 475	SEQ ID NO: 473
	GCATGGATGTVGGA	GCGTAATACGACTC	GCATGGATGTTGGACAAATTGGGGGGTGTCTTCGCCCCTCGTCCATCCACCGGG
	CAAATTGGG	ACTATAGGAGATTCA	CCTCACAAGTTGCGCGAATCCCTGCCTTTAGTGATTTTCCTTCGTAACAGGCTGAA
	SEQ ID NO: 476	AATTTGATGTAGTCA	GTATGCCCTTACAAACAGTGAAGTCACTAAAATTGTCATGCAAAGGTTGATCAAAG
	GCGTAATACGACTC	AGAATTTTAG	TTGATGGTAAAGTGAGGACTGATTCTAATTACCCTGCTGGTTTCATGGATGTCATT
	ACTATAGGGCATGG	SEQ ID NO: 477	ACTATTGAGAAGACTGGTGAATTTTTCCGTCTGATCTATGATGTTAAAGGAAGATT
	ATGTTGGACAAATTG	AGATTCAAATTTGAT	TGCTGTGCACCGTATTACAGCTGAAGAGGCAAAATACAAGTTGTGTAAAGTAAGG
	GG	GTAGTCAAGAATTTT	AGAGTCCAAACTGGTCCCAAAGGAATCCCATTTTTGGTAACACATGATGGCAGAA
		AG	CCATTCGTTACCCTGACCCCAACATCAAAGTGAATGACACAATTCAAATGGAAATT
			GCTACATCTAAAATTCTTGACTACATCAAATTTGAATCT

PC003	SEQ ID NO: 479	SEQ ID NO: 480	SEQ ID NO: 478
	CCCTAGACGTCCCT	GCGTAATACGACTC	CCCTAGACGTCCCTATGAAAAGGCCCGTCTGGATCAGGAATTGAAAATTATCGGC
	ATGAAAAGGCCC	ACTATAGGTTGACA	GCCTTTGGTTTACGAAACAAACGTGAAGTGTGGAGAGTAAAGTACACTTTGGCTA
	SED ID NO: 481	CGGCCAGGTCGGC	AAATCCGTAAAGCTGCTCGTGAACTGCTCACCCTAGAAGAAAAAGAGCCTAAAAG
	GCGTAATACGACTC	CACC	ATTGTTTGAAGGTAATGCACTTCTACGTCGTTTGGTGCGAATTGGTGTTCTGGATG
	ACTATAGGCCCTAG	SEQ ID NO: 482	AGAACAGGATGAAGCTTGATTATGTTTTGGGTCTGAAAATTGAAGATTTCTTGGAA
	ACGTCCCTATGAAA	TTGACACGGCCAGG	AGAAGGCTCCAAACTCAGGTGTTCAAATCTGGTCTGGCAAAGTCAATTCATCATG
	AGGCCC	TCGGCCACC	CTAGAGTACTGATTAGGCAGAGACACATCCGGGTGCGCAAGCAGGTGGTGAACA
			TCCCCTCGTTCATCGTGCGGCTGGACTCGCAGAAGCACATCGACTTCTCCCTGAA
			GTCGCCCTTCGGGGGTGGCCGACCTGGCCGTGTCAA

PC005	SEQ ID NO: 484	SEQ ID NO: 485	SEQ ID NO: 483
	ATCCTAATGAAATCA	GCGTAATACGACTC	ATCCTAATGAAATCAACGAAATCGCCAACACCAACTCAAGACAAAACATCCGTAAG
	ACGAAATCGCC	ACTATAGGTTCCCTA	CTCATCAAGGATGGTCTTATCATCAAGAAGCCAGTGGCAGTACACTCTAGGGCCC
	SEQ ID NO: 486	CGTTCCCTGGCCTG	GTGTACGCAAGAACACTGAAGCTAGAAGGAAGGGAAGGCATTGTGGATTTGGAAA
	GCGTAATACGACTC	CTTC	GAGGAAGGGTACGGCAAATGCCCGTATGCCTCAAAAGGAACTGTGGGTGCAGCG
	ACTATAGGATCCTAA	SEQ ID NO: 487	CATGCGCGTCCTCAGGCGCCTCCTCAAAAAGTACAGGGAGGCCAAGAAAATCGA
	TGAAATCAACGAAAT	TTCCCTACGTTCCCT	CCGCCATCTTTACCACGCCCTGTACATGAAAGCGAAGGGTAACGTGTTCAGGAAC
	CGCC	GGCCTGCTTC	AAGAGGGTCCTTATGGAGTACATCCACAAGAAGAAGGCAGAGAAGGCCAGGGCC
			AAGATGCTGTCTGACCAGGCTAACGCCAGGAGATTGAAGGTGAAGCAGGCCAGG
			GAACGTAGGGAA

PC010	SEQ ID NO: 489	SEQ ID NO: 490	SEQ ID NO: 488
	GCTCAGGCTATTAC	GCGTAATACGACTC	GCTCAGCCTATTACCGCCCAACGCGTTGATTGGATTGATCACGTTCGGAAAAATG
	CGCCCAACGC	ACTATAGGATGGAA	GTGCAAGTCCACGAACTGGGTACCGAAGGCTGCAGCAAGTCGTACGTGTTCTGT
	SEQ ID NO: 491	AATGAGTATCTGGA	GGAACGAAAGATCTCACCGCCAAGCAAGTCCAGGAGATGTTGGGCATTGGAAAA
	GCGTAATACGACTC	AGAAAG	GGGTCACCAAATCCCCAACAACAGCCAGGGCAACCTGGGCGGCCAGGGCAGAAT
	ACTATAGGGCTCAG	SEQ ID NO: 492	CCCCAAGCTGCCCCTGTACCACCGGGGAGCAGATTCTTGCAGCCCGTGTCAAAA
	CCTATTACCGCCCA	ATGGAAAATGAGTAT	TGCGACATGAACTTGACAGATCTGATCGGGGAGTTGCAGAAAGACCCTTGGCCC
	ACGC	CTGGAAGAAAG	GTACATCAGGGCAAAAGACCTCTTAGATCCACAGGCGCAGCATTGTCCATCGCTG
			TCGGCCTCTTAGAATGCACCTATCCGAATACGGGTGGCAGAATCATGATATTCTTA
			GGAGGACCATGCTCTCAGGGTCCCGGCCAGGTGTTGAACGACGATTTGAAGCAG
			CCCATCAGGTCCCATCATGACATACACAAAGACAATGCCAAGTACATGAAGAAGG
			CTATCAAACATTACGATCACTTGGCAATGCGAGCTGCCACCAACAGCCATTGCAT
			CGACATTTACTCCTGCGCCCTGGATCAGACGGGACTGATGGAGATGAAGCAGTG
			CTGCAATTCCACCGGAGGGCACATGGTCATGGGCGATTCCTTCAATTCCTCTCTA
			TTCAAACAAACCTTCCAGCGAGTGTTCTCAAAAGACCCGAAGAACGACCTCAAGA
			TGGCGTTCAACGCCACCTTGGAGGTGAAGTGTTCCAGGGAGTTAAAAGTCCAAG
			GGGGCATCGGCTCGTGCGTGTCCTTGAACGTTAAAAGCCCTCTGGTTTCCGATAC
			GGAACTAGGCATGGGGAATACTGTGCAGTGGAAACTTTGCACGTTGGCGCCGAG
			CTCTACTGTGGCGCTGTTCTTCGAGGTGGTTAACCAGCATTCGGCGCCCATACCA
			CAGGGAGGCAGGGGCTGCATCCAGCTCATCACCCAGTATCAGCACGCGAGCGG
			GCAAAGGAGGATCAGAGTGACCACGATTGCTAGAAATTGGGCGGACGCTACTGC
			CAACATCCACCACATTAGCGCTGGCTTCGACCAAGAAGCGGCGGCAGTTGTGAT
			GGCCCGAATGGCCGGTTACAAGGCGGAATCGGACGAGACTCCCGACGTGCTCA
			GATGGGTGGACAGGATGTTGATCAGGCTGTGCCAGAAGTTCGGAGAGTACAATA
			AAGACGATCCGAATTCGTTCAGGTTGGGGGAGAACTTCAGTCTGTATCCGCAGTT
			CATGTACCATTTGAGACGGTCGCAGTTTCTGCAGGTGTTCAATAATTCTCCTGATG
			AAACGTCGTTTTATAGGCACATGCTGATGCGTGAGGATTTGACTCAGTCTTTGATC
			ATGATCCAGCCGATTTTGTACAGTTACAGCTTCAACGGGCCGCCCGAGCCTGTGT
			TGTTGGACACAAGCTCTATTCAGCCGGATAGAATCCTGCTCATGGACACTTTCTTC
			CAGATACTCATTTTCCAT

PC014	SEQ ID NO: 494	SEQ ID NO: 495	SEQ ID NO: 493
	CTGATGTTCAAAAAC	GCGTAATACGACTC	CTGATGTTCAAAAACAAATCAAACACATGATGGCTTTCATTGAACAAGAAGCCAAT
	AAATCAAACACATG	ACTATAGGTGAGCG	GAGAAAGCAGAAGAAATTGATGCCAAGGCAGAGGAGGAATTCAACATTGAAAAAG
	SEQ ID NO: 496	ATCAGATCCAACCTA	GGCGTTTGGTCCAGCAACAGAGACTCAAGATCATGGAGTACTACGAGAAAAAGGA
	GCGTAATACGACTC	GCCTCC	GAAGCAAGTCGAACTTCAAAAGAAAATTCAGTCCTCTAATATGTTGAATCAGGCTC
	ACTATAGGCTGATG	SEQ ID NO: 497	GTTTGAAGGTGCTGAAAGTGAGAGAGGACCATGTCAGAGCAGTCCTGGAGGATG
	TTCAAAAACAAATCA	TGAGCGATCAGATC	CTCGTAAAAGTCTTGGTGAAGTAACCAAAGACCAAGGAAAATACTCCCAAATTTTG
	AACACATG	CAACCTAGCCTCC	GAGAGCCTAATCCTACAAGGACTGTTCCAGCTGTTCGAGAAGGAGGTGACGGTC
			CGCGTGAGACCGCAAGACAGGGACCTGGTCAGGTCCATCCTGCCCAACGTCGCT
			GCCAAATACAAGGACGCCACCGGCAAAGACATCCTACTCAAGGTGGACGATGAG
			TCGCACCTGTCTCAGGAGATCACCGGAGGCGTCGATTTGCTCGCTCAGAAGAAC
			AAGATCAAGATCAGCAACACGATGGAGGCTAGGTTGGATCTGATCGCTCA

PC016	SEQ ID NO: 499	SEQ ID NO: 500	SEQ ID NO: 498
	ACTGGTCATTCTTGA	GCGTAATACGACTC	ACTGGTCATTCTTGAGGATGTCAAGTTTCCAAAATTCAATGAAATTGTCCAGCTCA
	GGATGTCAAGT	ACTATAGGTTGGGC	AATTGGCAGATGGAACTCTACGATCTGGACAAGTTTTGGAAGTCAGTGGATCAAA
	SEQ ID NO: 501	ATAGTCAAGATGGG	GGCAGTTGTTCAGGTATTTGAAGGCACATCAGGTATTGATGCTAAGAACACGGTG
	GCGTAATACGACTC	GATCTGC	TGTGAGTTCACTGGAGATATTCTAAGAACTCCAGTATCAGAAGATATGCTGGGAC
	ACTATAGGACTGGT	SEQ ID NO: 502	GTGTCTTCAATGGATCAGGAAAACCCATTGATAAAGGTCCCCCGATCCTGGCTGA
	CATTCTTGAGGATGT	TTGGGCATAGTCAA	GGACTACCTCGACATCCAAGGACAGCCGATCAACCCGTGGTCGCGTATTTATCCC
	CAAGT	GATGGGGATCTGC	GAGGAAATGATCCAGACTGGGATCACGGCCATCGACGTGATGAACTCTATCGCCA
			GAGGGCAGAAGATTCCGATCTTCTCCGCCGCTGGGCTGCCCCACAATGAGATTG
			CAGCCCAGATTTGTAGGCAGGCTGGCTTGGTCAAAGTACCTGGCAAGTCTGTGCT
			GGATGACCATGAAGACAACTTTGCTATTGTGTTTGCTGCTATGGGTGTCAACATG
			GAAACTGCCAGGTTCTTCAAGCAGGACTTCGAAGAGAACGGCTCGATGGAGAAC
			GTGTGTCTGTTCTTGAACTTGGCCAACGATCCGACCATCGAGCGCATCATCACGC
			CGCGTTTGGCTCTGACGGCCGCCGAATTCTTGGCCTACCAGTGCGAGAAGCACG
			TGCTGGTCATCTTGACCGACATGTCGTCGTACGCGGAGGCGTTGCGTGAGGTGT
			CTGCCGCTCGAGAAGAAGTGCCCGGCCGTAGGGGTTTCCCCGGTTACATGTACA
			CCGATCTGGCCACCATTTACGAGCGCGCCGGTCGTGTGGAGGGCCGCAACGGC
			TCCATCACGCAGATCCCCATCTTGACTATGCCCAA

PC027	SEQ ID NO: 504	SEQ ID NO: 505	SEQ ID NO: 503
	CAAGCTAACTTGAAA	GCGTAATACGACTC	CAAGCTAACTTGAAAGTACTACCAGAAGGAGCTGAAATCAGAGATGGAGAACGTT
	GTACTACCAGAAGG	ACTATAGGTTTTGGA	TGCCAGTCACAGTAAAGGACATGGGAGCATGCGAGATTTACCCACAAACAATCCA
	SEQ ID NO: 506	ATTGAAGGCAATACT	ACACAACCCCAATGGGCGGTTTGTAGTGGTTTGTGGTGATGGAGAATACATAATA
	GCGTAATACGACTC	CGATCAG	TACACGGCTATGGCCCTTCGTAACAAAGCATTTGGTAGCGCTCAAGAATTTGTATG
	ACTATAGGCAAGCT	SEQ ID NO: 507	GGCACAGGACTCCAGTGAATATGCCATCCGCGAATCCGGATCCACCATTCGAATC
	AACTTGAAAGTACTA	TTTTGGAATTGAAGG	TTCAAGAATTTCAAAGAAAAAAAGAATTTCAAGTCCGACTTTGGTGCCGAAGGAAT
	CCAGAAGG	CAATACTCGATCAG	CTATGGTGGTTTTCTCTTGGGTGTGAAATCAGTTTCTGGCTTAGCTTTCTATGACT
			GGGAAACGCTTGAGTTAGTAAGGCGCATTGAAATACAGCCTAGAGCTATCTACTG
			GTCAGATAGTGGCAAGTTGGTATGCCTTGCTACCGAAGATAGCTATTTCATATTGT
			CCTATGACTCTGACCAAGTCCAGAAAGCTAGAGATAACAACCAAGTTGCTGAAGA
			TGGAGTGGAGGCTGCCTTTGATGTCCTAGGTGAAATAAATGAATCCGTAAGAACA
			GGTCTTTGGGTAGGAGACTGCTTCATTTACACAAACGCAGTCAACCGTATCAACTA
			CTTTGTGGGTGGTGAATTGGTAACTATTGCACATCTGGACCGTCCTCTATATGTCC
			TGGGCTATGTACCTAGAGATGACAGGTTATACTTGGTTGATAAAGAGTTAGGAGTA
			GTCAGCTATCNAATTGCTATTATCTGTACTCGAATATCAGACTGCAGTCATGCGAC
			GAGACTTCCCAACGGCTGATCGAGTATTGCCTTCAATTCCAAAA

TABLE 8-EV

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

EV005	SEQ ID NO: 577	SEQ ID NO: 578	SEQ ID NO: 576
	GACAAAACATCCGC	GCGTAATACGACTC	GACAAAACATCCGCAAACTGATTAAAGATGGTCTTATTATTAAAAAGCCTGTCGCG
	AAACTG	ACTATAGGCTCCTT	GTGCATTCTCGTGCACGTGTACGCAAAAATACTGAAGCCCGCAGGAAAGGTCGTC
	SEQ ID NO: 579	GCATCAGCTTGATC	ATTGTGGATTTGGTAAAAGGAAAGGAACTGCAAATGCTAGGATGCCCAGAAAGGA
	GCGTAATACGACTC	SEQ ID NO: 580	ATTATGGATTCAACGTATGAGAGTTCTCAGAAGGTTATTGAAGAAATATAGGGAAG
	ACTATAGGGACAAA	CTCCTTGCATCAGC	CTAAGAAAATTGATAGGCATTTATACCATGCTTTATATATGAAAGCTAAGGGAAAT
	ACATCCGCAAACTG	TTGATC	GTATTCAAGAATAAGAGAGTAATGATGGACTATATCCATAAAAAGAAGGCGGAGAA
			AGCACGTACAAAGATGCTCAATGATCAAGCTGATGCAAGGAG

EV009	SEQ ID NO: 582	SEQ ID NO: 583	SEQ ID NO: 581
	CAGGACTGAAGAAT	GCGTAATACGACTC	CAGGACTGAAGAATCTATAATAGGAACAAACCCAGGAATGGGTTTTAGGCCAATG
	CTATAATAGG	ACTATAGGCTGGAA	CCCGACAACAACGAAGAAAGTACCCTGATTTGGTTACAGGGTTCTAATAAAACAAA
	SEQ ID NO: 584	AGATGGGTAATACTTC	CTACGAAAAATGGAAAATGAATCTCCTCTCATATTTAGACAAGTATTACACTCCCG
	GCGTAATACGACTC	SEQ ID NO: 585	GAAAAATAGAAAAGGGAAATATTCCAGTAAAGCGCTGTTCATACGGAGAAAAATTG
	ACTATAGGCAGGAC	CTGGAAAGATGGGT	ATTAGGGGACAAGTATGTGATGTAGATGTGAGGAAATGGGAGCCGTGCACCCCG
	TGAAGAATCTATAAT	AATACTTC	GAAAATCATTTTGATTACCTCAGAAATGCGCCTTGTATATTTCTGAAGCTGAACAG
	AGG		GATATATGGATGGGAACCGGAGTACTACAACGATCCAAATGATCTTCCAGATGAT
			ATGCCGCAGCAGTTGAAGGACCATATACGTTATAATATCACCAATCCAGTGGAGA
			GAAATACCGTCTGGGTAACATGCGCAGGTGAAAATCCGGCAGACGTGGAGTACTT
			GGGCCCTGTGAAGTATTACCCATCTTTCCAG

EV010	SEQ ID NO: 587	SEQ ID NO: 588	SEQ ID NO: 586
	CCAATGGAGACTTG	GCGTAATACGACTC	CCAATGGAGACTTGAAGATGTCCTTCAACGCCATATTAGAAGTGAAGTGTTCTAGA
	AAGATGTC	ACTATAGGCTTCCCT	GAACTTAAAGTACAAGGAGGTATAGGTCCTTGTGTCTCTCTAAATGTCAAAAATCC
	SEQ ID NO: 589	CATCAACATGTGC	TCTTGTTTCTGATTTAGAAATAGGCATGGGTAACACAGTTCAGTGGAAACTGTGTA
	GCGTAATACGACTC	SEQ ID NO: 590	GCTTAAGTCCAAGCACTACGGTTGCCTTATTTTTTCGAAGTTGTTAATCAGCATGCA
	ACTATAGGCCAATG	CTTCCCTCATCAACA	GCACCCATTCCTCAAGGGGGACGTGGATGCATTCAGTTTATTACTCAATATCAGC
	GAGACTTGAAGATG	TGTGC	ATTCAAGTGGTCAGAAAAAAATAAGGGTAACTACAATAGCAAGAAATTGGGCGGA
	TC		TGCCACTGCAAATATTCACCATATTAGCGCTGGCTTTGACGAACAAACTGCGGCT
			GTTTTAATGGCGAGGATCGCTGTATATAGAGCAGAAACTGATGAGAGTTCAGATG
			TTCTCAGATGGGTTGACAGAATGTTGATACGATTGTGTCAGAAATTTGGAGAATAT
			AACAAAGATGACACCAACAGCTTCAGGCTCAGTGAAAACTTCAGCTTATATCCACA
			GTTTATGTATCATCTACGTCGTTCCCAATTTCTACAAGTGTTCAATAATTCACCAGA
			TGAAACTTCATTCTATAGGCACATGTTGATGAGGGAAG

EV015	SEQ ID NO: 592	SEQ ID NO: 593	SEQ ID NO: 591
	GTTAAGCCTCCAAG	GCGTAATACGACTC	GTTAAGCCTCCAAGGGGTATTCTCCTTTACGGGCCTCCCGGCACGGGGAAAACG
	GGGTATTC	ACTATAGGGAGCAC	CTGATCGCCAGGGCCGTTGCCAACGAAACTGGTGCGTTCTTCTTCCTCATCAATG
	SEQ ID NO: 594	AAAGAAGCCAAGTC	GGCCCGAGATTATGAGCAAGCTGGCCGGAGAATCCGAGAGCAATCTTAGAAAGG
	GCGTAATACGACTC	AG	CTTTTGAAGAGGCTGATAAAAACTCTCCTGCAATCATCTTTATCGACGAATTAGAC
	ACTATAGGGTTAAG	SEQ ID NO: 595	GCAATCGCTCCCAAGCGCGAGAAGACTCATGGTGAGGTAGAGAGACGCATCGTC
	CCTCCAAGGGGTAT	GAGCACAAAGAAGC	TCCCAACTGTTGACTTTGATGGACGGCATGAAGAAAAGTTCCCATGTGATCGTGA
	TC	CAAGTCAG	TGGCGGCCACGAACAGGCCCAATTCCATCGACCCTGCACTCAGACGTTTCGGCC
			GATTCGACAGAGAGATCGACATCGGTATCCCCGACGCTACTGGAAGATTAGAAGT
			ACTCAGAATACACACCAAAAACATGAAATTGGCTGACGATGTAGATTTGGAACAGA
			TTGCCGCAGAGACTCACGGTCATGTAGGTGCTGACTTGGCTTCTTTGTGCTC

EV016	SEQ ID NO: 597	SEQ ID NO: 598	SEQ ID NO: 596
	GGTGATCCTTGATA	GCGTAATACGACTC	GGTGATCCTTGATAGTGTTAAGTTTCCAAAATTTAACGAAATTGTACAGCTCAAGTT
	GTGTTAAG	ACTATAGGCCTCAG	ATCAGATGGAACAGTTAGGTCTGGACAAGTTTTGGAAGTCAGTGGACAGAAGGCG
	SEQ ID NO: 599	CATAAGATGACATG	GTTGTCCAAGTTTTTGAAGGCACCTCCGGAATTGATGCTAAAAACACTTTATGTGA
	GCGTAATACGACTC	SEQ ID NO: 600	ATTTACAGGAGATATCTTAAGAACTCCAGTGTCTGAAGATATGTTGGGTCGTGTGT
	ACTATAGGGGTGAT	CCTCAGCATAAGAT	TTAATGGATCTGGAAAGCCTATCGATAAAGGGCCGCCAATCTTAGCTGAAGATTTT
	CCTTGATAGTGTTAAG	GACATG	CTTGACATTCAAGGTCAACCTATAAATCCTTGGTCTCGTATCTATCCAGAAGAAAT
			GATCCAGACTGGTATTTCTGCGATTGATGTGATGAATTCCATTGCCAGAGGACAAA
			AGATTCCAATTTTCTCTGCAGCTGGTTTACCCCACAATGAAATCGCTGCTCAAATC
			TGTAGACAAGCTGGTCTTGTCAAAATCCCAGGGAAATCTGTCTTAGATGATCATGA
			AGACAACTTTGCTATCGTTTTCGCCGCTATGGGTGTCAATATGGAAACAGCCAGAT
			TCTTCAAGCAAGATTTTGAAGAGAATGGCTCTATGGAAAATGTGTGCCTATTTTTG
			AACTTGGCCAATGATCCTACCATTGAAAGAATTATAACACCCCGTTTGACTTTAAC
			AGCGGCTGAATTTATGGCATATCAATGTGAGAAGCATGTGTTAGTCATATTGACTG
			ACATGTCATCTTATGCTGAGG

TABLE 8-AG

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

AG001	SEQ ID NO: 769	SEQ ID NO: 770	SEQ ID NO: 768
	GCGTAATACGACTC	GATTTCCAGTTGGAT	GCATGGATGTTGGACAAATTGGGGGGTGTGTTCGCCCCCAGGCCCTCCACCGGG
	ACTATAGGGCATGG	GGTGTCG	CCACACAAGCTCAGGGAGTCCCTTCCATTAGTGATTTTCTTGCGTAACAGGTTGAA
	ATGTTGGACAAATTGG	SEQ ID NO: 772	GTACGCCCTGACAAACTGTGAGGTGACCAAGATCGTTATGCAGAGACTTATTAAG
	SEQ ID NO: 771	GCGTAATACGACTC	GTCGACGGCAAAGTCAGGACTGATCCTAACTATCCTGCTGGATTCATGGATGTGA
	GCATGGATGTTGGA	ACTATAGGGATTTCC	TCACCATTGAAAAAACTGGTGAATTCTTCCGTTTGATCTATGATGTTAAGGGAAGA
	CAAATTGG	AGTTGGATGGTGTCG	TTCACTATTCACAGGATCACTGCTGAAGAAGCAAAATACAAATTGTGCAAAGTCCG
			CAAGGTGCAAACCGGACCAAAAGGTATTCCATTCTTGGTCACCCACGATGGTAGG
			ACCATTAGGTACCCTGACCCAATGATCAAGGTAAACGACACCATCCAACTGGAAA
			TC

AG005	SEQ ID NO: 774	SEQ ID NO: 775	SEQ ID NO: 773
	GCGTAATACGACTC	CCTTTTGCCTTCTGG	CAACACCAACTCGAGGCAAAACATCCGTAAATTGATCAAGGATGGTTTGATCATTA
	ACTATAGGCAACAC	CGTTAG	AGAAACCGGTGGCAGTGCACTCTAGGGCTCGTGTCCGTAAAAACACAGAAGCTC
	CAACTCGAGGCAAA	SEQ ID NO: 777	GCAGGAAGGGAAGGCACTGCGGTTTCGGTAAGAGGAAAGGTACAGCGAACGCTC
	AC	GCGTAATACGACTC	GTATGCCTCAAAAGGAACTATGGATCCAAAGGATGCGTGTCTTGAGGCGTCTCCT
	SEQ ID NO: 776	ACTATAGGCCTTTTG	GAAAAAATACAGGGAAGCCAAAAAGATCGACAGGCATCTGTACCACGCCCTGTAC
	CAACACCAACTCGA	CCTTCTGGCGTTAG	ATGAAGGCCAAGGGTAACGTGTTCAAGAACAAGAGAGTGTTGATGGAATACATCC
	GGCAAAAC		ACAAGAAGAAGGCTGAGAAGGCCCGTGCCAAGATGTTGGCCGACCAAGCTAACG
			CCAGAAGGCAAAAGG

AG010	SEQ ID NO: 779	SEQ ID NO: 780	SEQ ID NO: 778
	GCGTAATACGACTC	GAAGGATGCCTGGT	CAAACTTTCCAAAGGGTGTTCGCGAAGGACCAGAATGGACATTTGAAGATGGCTT
	ACTATAGGCAAACTT	CATCTTTG	TCAACGGTACTTTGGAGGTGAAGTGCTCTAGGGAATTAAAAGTTCAAGGCGGTAT
	TCCAAAGGGTGTTCG	SEQ ID NO: 782	TGGCTCATGCGTGTCGCTAAATGTAAAAAGTCCTTTGGTAGCGGACACGGAAATA
	SEQ ID NO: 781	GCGTAATACGACTC	GGCATGGGAAACACCGTGCAATGGAAGATGTGCACCTTCAACCCTAGCACGACG
	CAAACTTTCCAAAG	ACTATAGGGAAGGA	ATGGCGCTGTTTTTCGAGGTGGTCAATCAGCATTCGGCCCCCATTCCTCAAGGTG
	GGTGTTCG	TGCCTGGTCATCTTTG	GTAGAGGATGTATACAGTTTATTACACAATATCAGCACTCGAGTGGCCAAAGGAG
			GATAAGGGTGACGACGATAGCGAGAAATTGGGCGGACGCATCGGCGAATATTCA
			CCACATCAGCGCGGGTTTCGATCAGGAACGTGCCGCGGTGATTATGGCCCGGAT
			GGCTGTTTATAGAGCGGAGACCGATGAGAGTCCCGATGTTTTAAGATGGGTCGAT
			CGGATGCTGATTCGTTTGTGTCAAAAGTTTGGAGAATATAACAAAGATGACCAGG
			CATCCTTC

AG014	SEQ ID NO: 784	SEQ ID NO: 785	SEQ ID NO: 783
	GCGTAATACGACTC	CAACTGTTGCGAAA	GAAAAGGCCGAGGAAATTGATGCCAAGGCGGAAGAAGAATTTAACATTGAAAAGG
	ACTATAGGGAAAAG	TCAGGTCC	GCCGCCTTGTGCAACAACAAAGATTGAAGATCATGGAATACTATGAGAAGAAGGA
	GCCGAGGAAATTGA	SEQ ID NO: 787	GAAGCAAGTCGAACTACAAAAGAAAATTCAATCCTCCAACATGCTGAACCAAGCC
	TG	GCGTAATACGACTC	CGTCTTAAGGTTCTGAAAGTCCGCGAAGATCATGTTAGAGCTGTATTGGATGAGG
	SEQ ID NO: 786	ACTATAGGCAACTG	CTCGCAAGAAGCTTGGTGAAGTCACCAGGGATCAAGGCAAATATGCCCAGATTCT
	GAAAAGGCCGAGGA	TTGCGAAATCAGGT	GGAATCTTTGATCCTTCAGGGACTCTACCAGCTTTTCGAGGCAAACGTGACCGTA
	AATTGATG	CC	CGCGTCCGCCCACAAGACAGAACCTTAGTCCAATCAGTGCTGCCAACCATCGCAA
			CCAAATACCGTGACGTCACCGGCCGAGATGTACACCTGTCCATCGATGACGAAAC
			TCAACTGTCCGAATCCGTAACCGGCGGAATCGAACTTTTGTGCAAACAAAACAAA
			ATTAAGGTCTGCAACACCCTGGAGGCACGTTTGGACCTGATTTCGCAACAGTTG

AG016	SEQ ID NO: 789	SEQ ID NO: 790	SEQ ID NO: 788
	GCGTAATACGACTC	CGACCGGCTCTTTC	GTGTTCAACGGATCAGGAAAACCCATTGACAAAGGTCCTCCAATCTTAGCCGAAG
	ACTATAGGGTGTTC	GTAAATG	ATTTCTTGGACATCCAAGGTCAACCCATCAACCCATGGTCGCGTATCTACCCGGA
	AACGGATCAGGAAA	SEQ ID NO: 792	AGAAATGATCCAGACCGGTATCTCCGCCATCGACGTGATGAACTCCATCGCGCGT
	ACC	GCGTAATACGACTC	GGGCAAAAAATCCCCATTTTCTCCGCGGCCGGTTTACCGCACAACGAAATCGCCG
	SEQ ID NO: 791	ACTATAGGCGACCG	CCCAAATCTGTAGACAGGCCGGTTTAGTCAAACTGCCGGGCAAATCGGTAATCGA
	GTGTTCAACGGATC	GCTCTTTCGTAAATG	CGATCACGAGGACAATTTCGCCATCGTGTTCGCCGCCATGGGTGTCAACATGGAA
	AGGAAAACC		ACCGCCCGTTTCTTCAAGCAGGACTTCGAAGAAAACGGTTCCATGGAGAACGTGT
			GTCTCTTCTTGAATTTGGCCAACGATCCCACCATCGAGAGAATCATCACGCCCCG
			TTTGGCTCTGACCGCCGCCGAATTTTTGGCTTATCAATGCGAGAAACACGTGCTG
			GTTATCTTAACTGATATGTCTTCTTACGCCGAGGCTTTGCGTGAAGTATCCGCCGC
			CAGAGAAGAAGTACCCGGACGTCGTGGGTTCCCCGGTTACATGTACACCGATTTG
			GCCACCATTTACGAAAGAGCCGGTCG

TABLE 8-TC

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

TC001	SEQ ID NO: 864	SEQ ID NO: 865	SEQ ID NO: 863
	GCGTAATACGACTC	GGTGTGCCCATTTG	CTGCGAAACAGGCTGAAGTATGCCTTGACCAACTCAGAAGTGACGAAGATTGTTA
	ACTATAGGCTGCGA	CATCCT	TGCAAAGATTGATTAAAGTTGACGGAAAAGTTAGGACAGACCCCAACTACCCCGC
	AACAGGCTGAAGTA	SEQ ID NO: 867	GGGTTTCATGGATGTTGTGACTATTGAGAAAACTGGGGAATTCTTCCGCTTGATTT
	TGC	GCGTAATACGACTC	ATGATGTTAAGGGAAGGTTCACAATCCATCGCATTACTGGAGAAGAGGCCAAATA
	SEQ ID NO: 866	ACTATAGGGGTGTG	TAAATTGTGCAAAGTGAAGAAAGTACAGACAGGCCCCAAGGGCATTCCCTTCTTG
	CTGCGAAACAGGCT	CCCATTTGCATCCT	GTGACCCGCGACGGACGCACTATCAGATACCCAGACCCCATGATCAAAGTGAAT
	GAAGTATGC		GACACCATTCAATTGGAGATTGCCACTTCGAAAATTCTTGATTTTATCAAATTTGAG
			TCCGGTAATTTGTGTATGATTACTGGAGGTCGTAACTTGGGGCGTGTCGGTACAG
			TGGTGAGCCGAGAACGTCACCCAGGTTCCTTCGACATCGTTCATATTAAGGATGC
			AAATGGGCACACC

TC002	SEQ ID NO: 869	SEQ ID NO: 870	SEQ ID NO: 868
	GCGTAATACGACTC	CTTTGTGAACAGCG	CATCCATGTTGAGGTGGGCATTTTTGAGGGCGTCCGCTGCGTTTTTCATCGTTTT
	ACTATAGGCATCCAT	GCCATC	GAGTACGGCTGTGTTGGTGTTGGCCCCCTCGAGGGCCTCCCGCTGCATCTCGAT
	GTTGAGGTGGGCA	SEQ ID NO: 872	GGTGCTGAGGGTGCCATCGATCTGCTGGAGCTGCTTTTCGTAGCGTTTCTTCCTC
	SEQ ID NO: 871	GCGTAATACGACTC	TTGATGGCCTGGATGGCCGCTGTTCACAAAG
	CATCCATGTTGAGG	ACTATAGGCTTTGTG
	TGGGCA	AACAGCGGCCATC

TC010	SEQ ID NO: 874	SEQ ID NO: 875	SEQ ID NO: 873
	GCGTAATACGACTC	ATGTCCTGGTACTT	ATGTCCTGGTACTTGAGGTTCCTCCATTGGGCGATTGTCTCACCGTGGAAAATCA
	ACTATAGGATGTAC	GAGGTTCCTCC	AAATTTGGAAAAATGTGTCCATGAGAAGGATCCGATCGGGTTGAATGGAACTAGT
	CATTTGCGCCGCTC	SEQ ID NO: 877	GTCGAGGAGGACGGGTTCAGGGGGGCCGTTGAAACTATAACTGTACAAAATCGG
	SEQ ID NO: 876	GCGTAATACGACTC	CTGGATCATAATGAGACTTTGGGTGAGGTCCTCCCGCATCAGCATGTGGCGGTAG
	ATGTACCATTTGCG	ACTATAGGATGTCCT	AACGAGGTCTCGTCTGGGGAGTTGTTGAAAACTTGGAGGAATTGGGAGCGGCGC
	CCGCTC	GGTACTTGAGGTTC	AAATGGTACAT
		CTCC

TC014	SEQ ID NO: 879	SEQ ID NO: 880	SEQ ID NO: 878
	GCGTAATACGACTC	ACAAGGCCGTACGA	CAACAGCGCTTGAAGATCATGGAATATTACGAGAAGAAGGAGAAACCGGTGGAAT
	ACTATAGGCAACAG	ATTTCTGG	TGCAGAAGAAAATTCAGTCGTCAAACATGCTGAACCAAGCCCGTTTGAAAGTATTA
	CGCTTGAAGATCAT	SEQ ID NO: 882	AAAGTGCGTGAAGACCACGTCCACAATGTGCTGGATGACGCCCGCAAACGTCTG
	GG	GCGTAATACGACTC	GGCGAAATCACCAATGACCAGGCGAGATATTCACAACTTTTGGAGTCTCTTATCCT
	SEQ ID NO: 881	ACTATAGGACAAGG	CCAGAGTCTCTACCAGTACTTGGGAATCAGTGATGAGTTGTTTGAGAACAATATAG
	CAACAGCGCTTGAA	CCGTACGAATTTCT	TGGTGAGAGTCAGGCAACAGGACAGGAGTATAATCCAGGGCATTCTCCCAGTTGT
	GATCATGG	GG	TGCGACGAAATACAGGGACGCCACTGGTAAAGACGTTCATCTTAAAATCGACGAT
			GAGAGCCACTTGCCATCCGAAACCACCGGAGGAGTGGTTTTGTATGCGCAAAAG
			GGTAAAATCAAGATTGACAACACCTTGGAGGCTCGTTTGGATTTAATTGCACAGCA
			ACTTGTGCCAGAAATTCGTACGGCCTTGT

TC015	SEQ ID NO: 884	SEQ ID NO: 885	SEQ ID NO: 883
	GCGTAATACGACTC	TCGGATTCGCCGGCC	CGATACAGTGTTGCTGAAAGGGAAGCGGCGGAAAGAGACCGTCTGCATTGTGCT
	ACTATAGGCGATAC	TAATTTAC	GGCCGACGAAAACTGCCCCGATGAGAAGATCCGGATGAACAGGATCGTCAGGAA
	AGTGTTGCTGAAAG	SEQ ID NO: 887	TAATCTACGGGTTAGGCTCTCTGACGTCGTCTGGATCCAGCCCTGTCCCGACGTC
	GGAAG	GCGTAATACGACTC	AAATACGGGAAGAGGATCCACGTTTTGCCCATCGATGACACGGTCGAAGGGCTC
	SEQ ID NO: 886	ACTATAGGTCGGAT	GTCGGAAATCTCTTCGAGGTGTACTTAAAACCATACTTCCTCGAAGCTTATCGACC
	CGATACAGTGTTGC	TCGCCGGCTAATTT	AATCCACAAAGGCGACGTTTTCATCGTCCGTGGTGGCATGCGAGCCGTTGAATTC
	TGAAAGGGAAG	AC	AAAGTGGTGGAAACGGAACCGTCACCATATTGTATCGTCGCCCCCGATACCGTCA
			TCCATTGTGACGGCGATCCGATCAAACGAGAAGAAGAGGAGGAAGCCTTGAACG
			CCGTCGGCTACGACGATATCGGCGGTTGTCGCAAACAACTCGCACAAATCAAAGA
			AATGGTCGAATTACCTCTACGCCACCCGTCGCTCTTCAAGGCCATTGGCGTGAAA
			CCACCACGTGGTATCCTCTTGTACGGACCTCCAGGTACCGGTAAAACTTTAATCG
			CACGTGCAGTGGCCAACGAAACCGGTGCTTTCTTCTTCTTAATCAACGGTCCCGA
			AATTATGAGTAAATTAGCCGGCGAATCCGA

TABLE 8-MP

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

MP001	SEQ ID NO: 1042	SEQ ID NO: 1043	SEQ ID NO: 1041
	GCGTAATACGACTC	CAATACCAACACGC	GTTTAAACGCACCCAAAGCATGGATGTTGGACAAATCGGGGGGTGTCTTCGCTCC
	ACTATAGGGTTTAAA	CCTAAATTGC	ACGTCCAAGCACCGGTCCACACAAACTTCGTGAATCACTACCGTTATTGATCTTCT
	CGCACCCAAAGCAT	SEQ ID NO: 1045	TGCGTAATCGTTTGAAGTATGCACTTACTGGTGCCGAAGTCACCAAGATTGTCATG
	GG	GCGTAATACGACTC	CAAAGATTAATCAAGGTTGATGGCAAAGTCCGTACCGACCCTAATTATCCAGCCG
	SEQ ID NO: 1044	ACTATAGGCAATAC	GTTTTATGGATGTTATATCTATCCAAAAGACCAGTGAGCACTTTAGATTGATCTATG
	GTTTAAACGCACCC	CAACACGCCCTAAA	ATGTGAAAGGTCGTTTCACCATCCACAGAATTACTCCTGAAGAAGCAAAATACAAG
	AAAGCATGG	TTGC	TTGTGTAAAGTAAAGAGGGTACAAACTGGACCCAAAGGTGTGCCATTTTTAACTAC
			TCATGATGGCCGTACTATTCGCTACCCTGACCCTAACATCAAGGTTAATGACACTA
			TTAGATACGATATTGCATCATCTAAAATTTTGGATCATATCCGTTTTGAAACTGGAA
			ACTTGTGCATGATAACTGGAGGTCGCAATTTAGGGCGTGTTGGTATTG

MP002	SEQ ID NO: 1047	SEQ ID NO: 1048	SEQ ID NO: 1046
	GCGTAATACGACTC	GCTGATTTAAGTGC	GGTGGCAAAAAGGAAGAGAAGGGACCATCAACCGAAGATGCGATACAAAAGCTT
	ACTATAGGGGTGGC	ATCTGCTGC	CGATCCACTGAAGAGATGCTGATAAAGAAACAAGAATTTTTAGAAAAAAAAATTGA
	AAAAAGAAGAGAA	SEQ ID NO: 1050	ACAAGAAGTAGCGATAGCCAAAAAAAATGGTACAACTAATAAACGAGCTGCATTG
	GG	GCGTAATACGACTC	CAAGCATTGAAGCGTAAGAAACGGTACGAACAACAATTAGCCCAAATTGATGGTA
	SEO ID NO: 1049	ACTATAGGGCTGAT	CCATGTTAACTATTGAACAACAGCGGGAGGCATTAGAAGGTGCCAACACAAATAC
	GGTGGCAAAAAGGA	TTAAGTGCATCTGCT	AGCAGTATTGACTACCATGAAAACTGCAGCAGATGCACTTAAATCAGC
	AGAGAAGG	GC

MP010	SEQ ID NO: 1052	SEQ ID NO: 1053	SEQ ID NO: 1051
	GCGTAATACGACTC	GCATTGGGAATCGA	CAGACCCTGTTCAGAATATGATGCATGTTAGTGCTGCATTTGATCAAGAAGCATCT
	ACTATAGGCAGACC	GTTTTGAG	GCCGTTTTAATGGCTCGTATGGTAGTGAACCGTGCTGAAACTGAGGATAGTCCAG
	CTGTTCAGAATATG	SEQ ID NO: 1055	ATGTGATGCGTTGGGCTGATCGTACGCTTATACGCTTGTGTCAAAAATTTGGTGAT
	SEQ ID NO: 1054	GCGTAATACGACTC	TATCAAAAAGATGATCCAAATAGTTTCCGATTGCCAGAAAACTTCAGTTTATATCCA
	CAGACCCTGTTCAG	ACTATAGGGCATTG	CAGTTCATGTATCATTTAAGAAGGTCTCAATTTCTACAAGTTTTTAATAATAGTCCT
	AATATG	GGAATCGAGTTTTG	GATGAAACATCATATTATAGGCACATGTTGATGCGTGAAGATGTTACCCAAAGTTT
		AG	AATCATGATACAGCCAATTCTGTATAGCTATAGTTTTAATGGTAGGCCAGAACCTG
			TACTTTTGGATACCAGTAGTATTCAACCTGATAAAATATTATTGATGGACACATTTT
			TCCATATTTTGATATTCCATGGAGAGACTATTGCTCAATGGAGAGCAATGGATTAT
			CAAAATAGACCAGAGTATAGTAACCTCAAGCAGTTGCTTCAAGCCCCCGTTGATG
			ATGCTCAGGAAATTCTCAAAACTCGATTCCCAATGC

MP016	SEQ ID NO: 1057	SEQ ID NO: 1058	SEQ ID NO: 1056
	GCGTAATACGACTC	CGTGGTGTAATGAT	GTTTTCAATGGCAGTGGAAAGCCGATAGATAAAGGACCTCCTATTTTGGCTGAAG
	ACTATAGGGTTTTCA	ACGCTC	ATTATTTGGATATTGAAGGCCAACCTATTAATCCATACTCCAGAACATATCCTCAAG
	ATGGCAGTGGAAAGC	SEQ ID NO: 1060	AAATGATTCAAACTGGTATTTCAGCTATTGATATCATGAACTCTATTGCTCGTGGAC
	SEQ ID NO: 1059	GCGTAATACGACTC	AAAAAATTCCAATATTTTCAGCTGCAGGTTTACCACATAATGAGATTGCTGCTCAAA
	GTTTTCAATGGCAGT	ACTATAGGCGTGGT	TTTGTAGACAAGCTGGTCTCGTTAAAAAACCTGGTAAATCAGTTCTTGACGATCAT
	GGAAAGC	GTAATGATACGCTC	GAAGACAATTTTGCTATAGTATTTGCTGCTATGGGTGTTAATATGGAAACAGCCAG
			ATTCTTTTAAAACAAGATTTTGAGGAAAATGGTTCAATGGAGAATGTTTGTTTGTTCTT
			GAATTTAGCTAATGATCCTACTATTGAGCGTATCATTACACCACG

MP027	SEQ ID NO: 1062	SEQ ID NO: 1063	SEQ ID NO: 1061
	GCGTAATACGACTC	CCAAAAATACCATCT	GCTCGTTTGTTTCCATCCAGAACTTCCCATCGTGTTAACTGGCTCAGAAGATGGTA
	ACTATAGGGCTCGT	GCTCCACC	CCGTCAGAATTTGGCATTCTGGTACTTATCGATTAGAATCATCATTAAACTATGGG
	TTGTTTCCATCCAGA	SEQ ID NO: 1065	TTAGAACGTGTATGGACAATCTGTTGCTTACGGGGATCTAATAATGTAGCTCTAGG
	AC	GCGTAATACGACTC	TTATGATGAAGGAAGTATAATGGTTAAAGTTGGTCGTGAAGAGCCAGCAATGTCAA
	SEQ ID NO: 1064	ACTATAGGCCAAAA	TGGATGTTCATGGGGGTAAAATTGTTTGGGCACGTCATAGTGAAATTCAACAAGCT
	GCTCGTTTGTTTCCA	ATACCATCTGCTCCA	AACCTTAAAGCGATGCTTCAAGCAGAAGGAGCCGAAATCAAAGATGGTGAACGTT
	TCCAGAAC	CC	TACCAATACAAGTTAAAGACATGGGTAGCTGTGAAATTTATCCACAGTCAATATCT
			CATAATCCGAATGGTAGATTTTTAGTAGTATGTGGTGATGGAGAGTATATTATATAT
			ACATCAATGGCTTTTGCGTAATAAAGCATTTGGCTCCGCTCAGGATTTTGTATGGTC
			TTCTGATTCTGAGTATGCCATTAGAGAAAATTCTTCTACAATCAAAGTTTTTAAAAA
			TTTTAAAGAAAAAAAGTCTTTTAAACCAGAAGGTGGAGCAGATGGTATTTTTGG

TABLE 8-NL

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence
ID
	5′ → 3′	5′ → 3′	5′ → 3′

NL001	SEQ ID NO: 1573	SEQ ID NO: 1574	SEQ ID NO: 1572
	GCGTAATACGACTCA	ACTGAGCTTCACA	GAAATCATGGATGTTGGACAAATTGGGTGGTGTGTATGCACCCCGACCCAGCACA
	CTATAGGGAAATCAT	CCCTTGCCC	GGTCCACACAAGCTGCGAGAATCTCTCCCACTTGTCATATTTTTGCGTAATCGGCT
	GGATGTTGGACAAAT	SEQ ID NO: 1576	CAAGTACGCTTTAACTAACTGTGAAGTGAAGAAAATTGTGATGCAGCGTCTCATCA
	TGG	GCGTAATACGACT	AGGTTGACGGCAAAGTGAGGACTGACCCCAACTATCCTGCAGGTTTTATGGACGT
	SEQ ID NO: 1575	CACTATAGGACTG	TGTTCAAATCGAAAAGACAAACGAGTTCTTCCGTTTGATCTATGATGTTAAGGGAC
	GAAATCATGGATGTT	AGCTTCACACCCT	GTTTCACCATCCACAGGATCACAGCTGAAGAAGCTAAGTACAAGCTGTGCAAAGT
	GGACAAATTGG	TGCCC	GAAGAGGGTTCAGACAGGACCCAAGGGCATTCCATTTTTGACCACTCACGATGGA
			CGCACCATCAGGTATCCAGACCCCTTAGTAAAAGTCAATGACACCATCCAATTGG
			ACATTGCCACATCCAAAATCATGGACTTCATCAGATTCGACTCTGGTAACCTGTGT
			ATGATCACTGGAGGTCGTAACTTGGGTCGTGTGGGCACTGTCGTGAACAGGGAG
			CGACACCCGGGGTCTTTCGACATCGTGCACATCAAGGACGTGTTGGGACACACTT
			TTGCCACTAGGTTGAACAACGTTTTCATCATCGGCAAGGGTAGTAAAGCATACGT
			GTCTCTGCCCAAGGGCAAGGGTGTGAAGCTCAGT

NL002	SEQ ID NO: 1578	SEQ ID NO: 1579	SEQ ID NO: 1577
	GCGTAATACGACTCA	CTGATCCACATCC	GATGAAAAGGGCCCTACAACTGGCGAAGCCATTCAGAAACTACGCGAAACAGAG
	CTATAGGGATGAAAA	ATGTGTTGATGAG	GAAATGCTGATAAAGAAACAAGACTTTTAGAAAAGAAAATTGAAGTTGAAATTGG
	GGGCCCTACAACTGGC	SEQ ID NO: 1581	AGTTGCCAGGAAGAATGGAACAAAAAACAAAAGAGCCGCGATCCAGGCACTCAAA
	SEQ ID NO: 1580	GCGTAATACGACT	AGGAAGAAGAGGTATGAAAAGCAATTGCAGCAGATCGATGGAACGTTATCAACAA
	GATGAAAAGGGCCCT	CACTATAGGCTGA	TTGAGATGCAGAGAGAGGCCCTCGAAGGAGCCAACACGAATACGGCCGTACTGC
	ACAACTGGC	TCCACATCCATGT	AAACTATGAAGAACGCAGCAGATGCTCTCAAAGCGGCTCATCAACACATGGATGT
		GTTGATGAG	GGATCAG

NL003	SEQ ID NO: 1583	SEQ ID NO: 1584	SEQ ID NO: 1582
	GCGTAATACGACTCA	TTGACGCGACCAG	TCCGCGTCGTCCTTACGAGAAGGCACGTCTCGAACAGGAGTTGAAGATCATCGG
	CTATAGGTCCGCGTC	GTCGGCCAC	AGAGTATGGACTCCGTAACAAGCGTGAGGTGTGGAGAGTCAAATACGCCCTGGC
	GTCCTTACGAGAAGGC	SEQ ID NO: 1586	CAAGATTCGTAAGGCCGCTCGTGAGCTGTTGACTCTGGAAGAGAAGGACCAGAA
	SEQ ID NO: 1585	GCGTAATACGACT	ACGTTTGTTTGAAGGTAACGCCCTGCTGCGTCGCCTGGTGCGTATTGGAGTGTTG
	TCCGCGTCGTCCTTA	CACTATAGGTTGA	GACGAAGGAAGAATGAAGCTCGATTACGTCTTGGGTTTAAAAATTGAAGATTTCCT
	CGAGAAGGC	CGCGACCAGGTCG	TGAACGTCGTCTACAGACTCAGGTGTACAAACTCGGTTTGGCCAAGTCCATCCAT
		GCCAC	CACGCCCGTGTACTCATCAGACAAAGACATATCAGAGTGCGCAAACAAGTAGTGA
			ACATTCCGAGCTTTGTGGTGCGCCTGGACTCGCAGAAGCACATTGACTTCTCGCT
			GAAGTCGCCGTTCGGCGGTGGCCGACCTGGTCGCGTCAA

NL004	SEQ ID NO: 1588	SEQ ID NO: 1589	SEQ ID NO: 1587
	GCGTAATACGACTCA	CTGTTGTTGACTGT	GGAGTTGGCTGCTGTAAGAACTGTCTGCTCTCACATCGAAAACATGCTGAAGGGA
	CTATAGGGGAGTTGG	TGGATGAGG	GTCACAAAGGGATTCCTGTACAAGATGCGTGCCGTGTACGCCCATTTCCCCATCA
	CTGCTGTAAGAACTG	SEQ ID NO: 1591	ACTGTGTGACGACCGAGAACAACTCTGTGATCGAGGTGCGTAACTTCCTGGGCG
	SEQ ID NO: 1590	GCGTAATACGACT	AGAAGTACATCCGACGGGTGAGGATGGCGCCCGGCGTCACTGTTACCAACTCGA
	GGAGTTGGCTGCTGT	CACTATAGGCTGT	CAAAGCAGAAGGACGAGCTCATCGTCGAAGGAAACAGCATAGAGGACGTGTCAA
	AAGAACTG	TGTTGACTGTTGG	GATCAGCTGCCCTCATCCAACAGTCAACAACAG
		ATGAGG

NL005	SEQ ID NO: 1593	SEQ ID NO: 1594	SEQ ID NO: 1592
	GCGTAATACGACTCA	CCTTCGCTTCTTG	CGCAAACACAAATTCACGTCAAAGCATCAGGAAGCTGATCAAAGACGGTCTTATC
	CTATAGGCGCAAACA	GCCTCCTTGAC	ATCAAGAAACCGGTTGCAGTACATTCACGTGCTCGCGTTCGTAAAAACACTGAAG
	CAAATTCACGTCAAAGC	SEQ ID NO: 1596	CCAGGAGGAAAGGCAGACATTGTGGCTTTGGTAAGAGGAAAGGTACAGCCAACG
	SEQ ID NO: 1595	GCGTAATACGACT	CCCGTATGCCACAAAAGGTTCTATGGGTGAATCGTATGCGTGTCTTGAGAAGACT
	CGCAAACACAAATTCA	CACTATAGGCCTT	GTTGAAAAAATACAGACAAGATAAGAAAATCGACAGGCATCTGTACCATCACCTTT
	CGTCAAAGC	CGCTTCTTGGCCT	ACATGAAGGCTAAGGGTAACGTATTCAAGAACAAGCGTGTATTGATGGAGTTCATT
		CCTTGAC	CATAAGAAGAAGGCCGAGAAAGCAAGAATGAAGATGTTGAACGACCAGGCTGAA
			GCTCGCAGACAAAAGGTCAAGGAGGCCAAGAAGCGAAGG

NL006	SEQ ID NO: 1598	SEQ ID NO: 1599	SEQ ID NO: 1597
	GCGTAATACGACTCA	CGAGATGGGATAG	GTGCTTGTGTCAAGTGGTGTGGTGGAGTACATTGACACCCTGGAGGAGGAGACG
	CTATAGGGTGCTTGT	CGTGAGG	ACCATGATAGCGATGTCGCCGGATGACCTGCGTCAGGACAAGGAGTATGCCTAC
	GTCAAGTGGTGTGG	SEQ ID NO: 1601	TGTACCACCTACACGCACTGCGAGATCCACCCGGCCATGATACTCGGTGTGTGC
	SEQ ID NO: 1600	GCGTAATACGACT	GCCTCTATTATTCCCTTCCCCGATCACAACCAAAGTCCCAGGAACACCTATCAGA
	GTGCTTGTGTCAAGT	CACTATAGGCGAG	GCGCTATGGGGAAACAGGCGATGGGCGTGTACATCACCAACTTCCACGTGCGAA
	GGTGTGG	ATGGGATAGCGTG	TGGACACGCTGGCTCACGTGCTGTTCTACCCGCACAAGCCACTGGTCACCACTC
		AGG	GCTCCATGGAGTACCTGCGCTTCAGGGAGCTTCCTGCCGGCATCAACTCTGTGG
			TCGCCATCGCCTGCTACACTGGATACAACCAGGAGGACAGTGTCATTCTCAACGC
			CTCCGCTGTCGAGCGCGGATTCTTCAGATCGGTTTTCTTCCGATCTTACAAAGAT
			GCAGAATCGAAGCGTATTGGCGACCAAGAGGAGCAATTCGAGAAGCCCACCAGA
			CAGACGTGTCAGGGAATGAGGAATGCCATTTATGACAAATTGGACGATGATGGCA
			TCATTGCTCCCGGTCTGAGAGTGTCTGGTGACGATGTGGTTATTGGCAAAACCAT
			AACACTGCCCGATAATGATGACGAGCTGGAAGGTACAACAAAGAGGTTCACGAAG
			AGAGATGCCAGTACTTTCCTGCGTAACAGTGAGACGGGAATCGTCGACCAAGTCA
			TGTTAACCTTGAACTCTGAGGGTTACAAGTTCTGCAAAATTCGAGTCAGGTCTGTG
			CGTATCCCGCAGATTGGCGATAAGTTCGCTTCCCGACATGGCCAAAAAGGAACGT
			GTGGAATACAGTATCGTCAAGAGGACATGCCTTTTACAAGCGAGGGAATCGCACC
			GGATATTATTATCAATCCTCACGCTATCCCATCTCG

NL007	SEQ ID NO: 1603	SEQ ID NO: 1604	SEQ ID NO: 1602
	GCGTAATACGACTCA	CCACGGTGAATAG	TGAGAGCAATCCTTGACTGTGGTTTTGAACATCCATCTGAAGTACAACATGAATGC
	CTATAGGTGAGAGCA	CCACTGC	ATTCCTCAAGCTGTACTTGGAATGGACATATTGTGTCAAGCGAAATCCGGTATGG
	ATCCTTGACTGTGG	SEQ ID NO: 1606	GAAAAACTGCTGTATTTGTGTTGGCGACATTACAGCAAATTGAACCAACTGACAAC
	SEQ ID NO: 1605	GCGTAATACGACT	CAAGTCAGTGTATTGGTCATGTGTCATACCAGAGAGCTTGCATTCCAAATCAGCAA
	TGAGAGCAATCCTTG	CACTATAGGCCAC	AGAGTATGAACGATTTTCGAAATGTATGCCAAATATCAAGGTTGGAGTTTTCTTCG
	ACTGTGG	GGTGAATAGCCAC	GCGGACTGCCGATTCAGAGGGATGAGGAGACGTTGAAATTGAACTGTCCTCACAT
		TGC	CGTGGTTGGAACACCCGGACGAATTTTGGCGTTGGTACGCAACAAGAAGCTGGA
			CCTCAAGCATCTCAAGCACTTTGTCCTTGACGAATGTGACAAAATGTTGGAACTGT
			TAGATATGCGAAGAGATGTGCAGGAAATATTCCGAAACACGCCGCACAGCAAACA
			AGTCATGATGTTCAGTGCAACTCTCAGCAAAGAAATTCGTCCAGTCTGCAAGAAAT
			TCATGCAAGATCCGATGGAAGTGTACGTTGATGACGAGGCCAAGCTGACGCTTCA
			CGGCCTGCAGCAGCACTATGTCAAACTCAAAGAAAACGAAAAGAACAAAAAGTTA
			TTTGAATTACTTGACATACTTGAATTCAACCAGGTTGTTATATTTGTGAAGTCAGTG
			CAGCGCTGCATGGCCCTATCGCAACTCCTAACAGAGCAGAACTTCCCTGCAGTG
			GCTATTCACCGTGG

NL008	SEQ ID NO: 1608	SEQ ID NO: 1609	SEQ ID NO: 1607
	GCGTAATACGACTCA	GAGCGAGTCTACA	GATGCTGGAGACCTGGAGGTGTATTAGATGTTTCAAACAGTTTTGCAGTTCCATTT
	CTATAGGGATGCTGG	AAATTGCCG	GATGAGGACGACAAAGAAAAGAATGTTTGGTTCTTAGACCATGATTACTTGGAAAA
	AGACCTGGAGGTG	SEQ ID NO: 1611	CATGTTCGGGATGTTCAAGAAAGTTAATGCTAGAGAAAAGGTTGTGGGTTGGTAC
	SEQ ID NO: 1610	GCGTAATACGACT	CATACTGGACCCAAACTCCACCAAAACGATGTTGCAATCAATGAGTTGATTCGTCG
	GATGCTGGAGACCTG	CACTATAGGGAGC	TTACTGTCCAAACTGTGTCTTAGTCATAATCGATGCCAAGCCTAAAGATTTGGGTC
	GAGGTG	GAGTCTACAAAATT	TACCTACAGAGGCATACAGAGTCGTTGAAGAAATCCATGATGATGGATCGCCAAC
		GCCG	ATCAAAAACATTTGAACATGTGATGAGTGAGATTGGGGCAGAAGAGGCTGAGGAG
			ATTGGCGTTGAACATCTGTTGAGAGACATCAAAGATACAACAGTCGGGTCACTGT
			CACAGCGCGTCACAAATCAGCTGATGGGCTTGAAGGGCTTGCATCTGCAATTACA
			GGATATGCGAGACTATTTGAATCAGGTTGTCGAAGGAAAGTTGCCAATGAACCAT
			CAAATCGTTTACCAACTGCAAGACATCTTCAACCTTCTACCCGATATCGGCCACGG
			CAATTTTGTAGACTCGCTC

NL009	SEQ ID NO: 1613	SEQ ID NO: 1614	SEQ ID NO: 1612
	GCGTAATACGACTCA	GTGTAAGGGTAGA	GCGACTATGATCGACCGCCGGGACGCGGTCAGGTGTGCGACGTCGACGTCAAG
	CTATAGGGCGACTAT	AGTAGCCCGG	AACTGGTTTCCCTGCACCTCTGAGAACAATTTCAACTACCATCAATCGAGCCCTTG
	GATCGACCGCC	SEQ ID NO: 1616	TGTTTTTCTCAAACTGAACAAGATAATTGGTTGGCAACCGGAGTACTACAATGAGA
	SEQ ID NO: 1615	GCGTAATACGACT	CTGAAGGCTTTCCAGATAATATGCCAGGTGACCTCAAGCGACACATTGCCCAACA
	GCGACTATGATCGAC	CACTATAGGGTGT	GAAGAGTATCAACAAGCTGTTTATGCAACAATCTGGATAACTTGCGAAGGAGAG
	CGCC	AAGGGTAGAAGTA	GGTCCTCTAGACAAGGAGAATGCAGGGGAGATCCAGTACATCCCTAGACAGGGA
		GCCCGG	TTTCCGGGCTACTTCTACCCTTACAC

NL010	SEQ ID NO: 1618	SEQ ID NO: 1619	SEQ ID NO: 1617
	GCGTAATACGACTCA	GCAACTCCAGTAG	GCTTGTTGTTCCCGTTGGATGTCTGTATCAACCTTTGAAGGAGAGACCTGATCTAC
	CTATAGGGCTTGTTGT	ATCGGAGAGGTC	CGCCTGTACAGTACGATCCAGTTCTTTGTACTAGGAATACTTGTCGTGCAATTCTG
	TCCCGTTGGATGTC	SEQ ID NO: 1621	AATCCATTGTGCCAAGTCGACTATCGAGCCAAGCTATGGGTCTGCAACTTTTGTTT
	SEQ ID NO: 1620	GCGTAATACGACT	CCAGAGGAATCCTTCCCCCCTCAATATGCAGCTATTTCGGAGCAGCATCAACCA
	GCTTGTTGTTCCCGTT	CACTATAGGGCAA	GCAGAACTGATACCTTCATTTTCCACCATCGAATACATCATTACCAGAGCGCAAAC
	GGATGTC	CTCCAGTAGATCG	GATGCCGCCGATGTTCGTGCTGGTGGTGGACACATGTCTGGACGACGAGGAGCT
		GAGAGGTC	GGGAGCTTTGAAGGACTCACTGCAGATGTCGCTGTCGCTGCTGCCGCCCAATGC
			ACTCATCGGTCTCATCACGTTCGGCAAAATGGTGCAGGTGCACGAGCTTGGCTGC
			GACGGCTGCTCGAAGAGCTACGTGTTCCGTGGCGTGAAGGACCTGACTGCCAAG
			CAGATCCAGGACATGTTGGGCATTGGCAAGATGGCCGCCGCTCCACAGCCCATG
			CAACAGCGCATTCCCGGCGCCGCTCCCTCCGCACCTGTCAACAGATTTCTTCAGC
			CTGTCGGAAAGTGCGATATGAGTTTAACTGATCTGCTTGGGGAATTGCAAAGAGA
			TCCATGGAATGTGGCTCAGGGCAAGAGACCTCTCCGATCTACTGGAGTTGC

NL011	SEQ ID NO: 1623	SEQ ID NO: 1624	SEQ ID NO: 1622
	CCCACTTTCAAGTGY	GTCCATTGTGACC	GTTGCCACCCTTGGAGTTGAAGTTCACCCCCTTGTATTTCACACAAACAGAGGTG
	GTRYTRGTCGG	TCGGGAGG	TGATTAGGTTCAATGTGTGGGACACAGCTGGCCAGGAAAAGTTCGGTGGACTTCG
	SEQ ID NO: 1625	SEQ ID NO: 1626	TGATGGATATTACATTCAGGGACAATGCGCCATCATTATGTTTGACGTAACGTCAA
	GTTGCCACCCTTGGA	GCGTAATACGACT	GAGTCACCTACAAGAACGTTCCCAACTGGCACAGAGATTTAGTGAGGGTTTGCGA
	GTTGAAG	CACTATAGGGTCC	AAACATTCCCATTGTACTATGCGGCAACAAAGTAGACATCAAGGACAGGAAAGTC
		ATTGTGACCTCGG	AAGGCCAAGAGCATAGTCTTCCATAGGAAGAAGAACCTTCAGTACTACGACATCA
		GAGG	GTGCGAAAAGCAACTACAACTTCGAGAAGCCGTTCCTGTGGTTGGCAAAGAAGCT
			GATCGGTGACCCCAACCTGGAGTTCGTCGCCATGCCCGCCCTCCTCCCACCCGA
			GGTCACAATGGAC

NL012	SEQ ID NO: 1628	SEQ ID NO: 1629	SEQ ID NO: 1627
	GCGTAATACGACTCA	GAATTTCCTCTTGA	GCAGCAGACGCAGGCACAGGTAGACGAGGTTGTCGATATAATGAAAACAAACGTT
	CTATAGGGCAGCAGA	GTTTGCCAGCTTG	GAGAAAGTATTGGAGAGGGATCAAAAACTATCAGAATTGGATGATCGAGCAGATG
	CGCAGGCACAGGTAG	SEQ ID NO: 1631	CTCTACAGCAAGGCGCTTCACAGTTTGAACAGCAAGCTGGCAAACTCAAGAGGAA
	SEQ ID NO: 1630	GCGTAATACGACT	ATTC
	GCAGCAGACGCAGGC	CACTATAGGGAAT
	ACAGGTAG	TTCCTCTTGAGTTT
		GCCAGCTTG

NL013	SEQ ID NO: 1633	SEQ ID NO: 1634	SEQ ID NO: 1632
	GCGTAATACGACTCA	GGCAACGGCTCTC	CGCAGAGCAAGTCTACATCTCTTCACTGGCCTTATTGAAAATGCTTAAGCACGGTC
	CTATAGGCGCAGAGC	TTGGATAG	GCGCCGGTGTTCCCATGGAAGTTATGGGCCTAATGCTGGGCGAATTTGTAGACG
	AAGTCTACATCTCTTC	SEQ ID NO: 1636	ACTACACTGTGCGTGTCATTGATGTATTCGCTATGCCACAGAGTGGAACGGGAGT
	SEQ ID NO: 1635	GCGTAATACGACT	GAGTGTGGAGGCTGTAGACCCGGTGTTCCAAGCGAAGATGTTGGACATGCTAAA
	CGCAGAGCAAGTCTA	CACTATAGGGGCA	GCAGACAGGACGGCCCGAGATGGTGGTGGGCTGGTACCACTCGCACCCGGGCT
	CATCTCTTC	ACGGCTCTCTTGG	TCGGCTGCTGGCTGTCGGGTGTCGACATCAACACGCAGGAGAGCTTCGAGCAAC
		ATAG	TATCCAAGAGAGCCGTTGCC

NL014	SEQ ID NO: 1638	SEQ ID NO: 1639	SEQ ID NO: 1637
	GCGTAATACGACTCA	GAGCGCGACTCTA	CATTGAGCAAGAAGCCAATGAGAAAGCCGAAGAGATCGATGCCAAGGCCGAGGA
	CTATAGGCATTGAGC	ATCTCGG	AGAATTCAACATTGAAAAGGGAAGGCTCGTACAGCACCAGCGCCTTAAAATCATG
	AAGAAGCCAATGAG	SEQ ID NO: 1641	GAGTACTATGACAGGAAAGAGAAGCAGGTTGAGCTCCAGAAAAAAATCCAATCGT
	SEQ ID NO: 1640	GCGTAATACGACT	CAAACATGCTGAACCAAGCGCGTCTGAAGGCACTGAAGGTGCGCGAAGATCACG
	CATTGAGCAAGAAGC	CACTATAGGGAGC	TGAGAAGTGTGCTCGAAGAATCCAGAAAACGTCTTGGAGAAGTAACCAGAAACCC
	CAATGAG	GCGACTCTAATCT	AGCCAAGTACAAGGAAGTCCTCCAGTATCTAATTGTCCAAGGACTCCTGCAGCTG
		CGG	CTAGAATCAAACGTAGTACTGCGCGTGCGCGAGGCTGACGTGAGTCTGATCGAG
			GGCATTGTTGGCTCATGCGCAGAGCAGTACGCGAAGATGACCGGCAAAGAGGTG
			GTGGTGAAGCTGGACGCTGACAACTTCCTGGCCGCCGAGACGTGTGGAGGCGTC
			GAGTTGTTCGCCCGCAACGGCCGCATCAAGATCCCCAACACCCTCGAGTCCAGG
			CTCGACCTCATCTCCCAGCAACTTGTGCCCGAGATTAGAGTCGCGCTC

NL015	SEQ ID NO: 1643	SEQ ID NO: 1644	SEQ ID NO: 1642
	GCGTAATACGACTCA	GGCCAAAGCGCCT	CTGCGAGTGCGCTTGTCCGACATTGTCTCGATCCAGCCTTGCCCAGACGTCAAGT
	CTATAGGCTGCGAGT	AAGCGC	ATGGAAAGCGTATCCATGTGCTGCCCATTGATGATACCGTTGAGGGTCTTACAGG
	GCGCTTGTCCG	SEQ ID NO: 1646	AAATCTGTTCGAAGTGTATTTGAAGCCATACTTCCTGGAAGCATACAGGCCAATTC
	SEQ ID NO: 1645	GCGTAATACGACT	ACAAGGATGATGCATTCATTGTTCGCGGAGGTATGAGAGCGGTCGAATTCAAGGT
	CTGCGAGTGCGCTTG	CACTATAGGGGCC	GGTTGAAACAGATCCATCGCCCTACTGCATTGTCGCGCCAGACACCGTCATCCAT
	TCCG	AAAGCGCCTAAGC	TGTGAGGGAGACCCCATCAAACGTGAGGATGAAGAAGACGCAGCAAACGCAGTC
		GC	GGCTACGACGACATTGGAGGCTGCAGAAAGCAGCTGGCGCAGATCAAAGAGATG
			GTGGAGTTGCCGCTGAGACATCCCAGTCTGTTCAAGGCGATCGGCGTGAAGCCG
			CCACGAGGCATCCTGCTGTACGGACCACCGGGAACCGGAAAGACGTTGATAGCG
			CGCGCCGTCGCCAACGAAACGGGCGCCTTCTTCTTCCTCATCAACGGACCCGAG
			ATTATGAGCAAATTGGCCGGCGAGTCGGAGAGTAACCTGCGCAAAGCTTTCGAG
			GAAGCGGACAAAAACGCACCGGCCATCATCTTCATCGATGAGCTGGACGCAATC
			GCGCCAAAACGCGAGAAGACGCACGGCGAGGTGGAGCGACGCATCGTGTCGCA
			GCTGCTGACGCTGATGGACGGTCTCAAGCAGAGCTCGCACGTGATTGTCATGGC
			CGCCACCAATCGGCCCAACTCGATCGATGCCGCGCTTAGGCGCTTTGGCC

NL016	SEQ ID NO: 1648	SEQ ID NO: 1649	SEQ ID NO: 1647
	GCGTAATACGACTCA	GATGGAGCCGTTG	GACGCCAGTATCAGAAGACATGCTTGGTCGTGTATTCAACGGAAGTGGTAAGCCC
	CTATAGGGACGCCAG	CGACC	ATCGACAAAGGACCTCCCATTCTTGCTGAGGATTATCTCGACATTCAAGGTCAACC
	TATCAGAAGACATGC	SEQ ID NO: 1651	CATCAATCCTTGGTCGCGTATCTATCCCGAGGAAATGATCCAGACTGGAATTTCA
	SEQ ID NO: 1650	GCGTAATACGACT	GCCATCGACGTCATGAACTCGATTGCTCGTGGCCAGAAAATCCCCATCTTTTCAG
	GACGCCAGTATCAGA	CACTATAGGGATG	CTGCCGGTCTACCTCACAACGAAATTGCTGCTCAAATCTGTAGACAGGCTGGTCT
	AGACATGC	GAGCCGTTGCGACC	TGTCAACTGCCAGGAAAGTCAGTTCTCGATGACTCTGAGGACAACTTTGCTATTG
			TATTCGCAGCCATGGGAGTCAACATGGAAACTGCTCGATTCTTCAAACAGGATTTC
			GAGGAGAACGGCTCTATGGAGAACGTGTGCCTGTTCTTGAACCTGGCGAACGAC
			CCGACGATCGAGCGTATCATCACACCACGCCTGGCGCTGACGGCCGCCGAGTTC
			CTGGCCTACCAGTGCGAGAAGCACGTGCTCGTCATCCTCACCGACATGAGCTCC
			TACGCCGAGGCGCTGCGAGAGGTGTCCGCCGCCCGCGAGGAGGTGCCCGGCC
			GTCGTGGTTTCCCCGGTTACATGTACACCGATCTGGCCACCATCTACGAGCGCGC
			CGGACGAGTCGAGGGTCGCAACGGCTCCATC

NL018	SEQ ID NO: 1653	SEQ ID NO: 1654	SEQ ID NO: 1652
	GCGTAATACGACTCA	GCAATACAGCCGA	GCAAATGCCTGTGCCACGCCCACAAATAGAAAGCACACAACAGTTTATTCGATCC
	CTATAGGGCAAATGC	CCACTCCG	GAGAAAACAACATACTCGAATGGATTCACCACCATTGAGGAGGACTTCAAAGTAG
	CTGTGCCACGC	SEQ ID NO: 1656	ACACTTTCGAATACCGTCTTCTGCGCGAGGTGTCGTTCCGCGAATCTCTGATCAG
	SEQ ID NO: 1655	GCGTAATACGACT	AAACTACTTGCACGAGGCGGACATGCAGATGTCGACGGTGGTGGACCGAGCATT
	GCAAATGCCTGTGCC	CACTATAGGGCAA	GGGTCCCCCCTCGGCGCCACACATCCAGCAGAAGCCGCGCAACTCAAAAATCCA
	ACGC	TACAGCCGACCAC	GGAGGGCGGCGATGCCGTCTTTTCCATCAAGCTCAGCGCCAACCCCAAGCCTCG
		TCCG	GCTGGTCTGGTTCAAGAACGGTCAGCGCATCGGTCAGACGCAGAAACACCAGGC
			CTCCTACTCCAATCAGACCGCCACGCTCAAGGTCAACAAAGTCAGCGCTCAAGAC
			TCCGGCCACTACACGCTGCTTGCTGAAAATCCGCAAGGATGTACTGTGTCCTCAG
			CTTACCTAGCTGTCGAATCAGCTGGCACTCAAGATACAGGATACAGTGAGCAATA
			CAGCAGACAAGAGGTGGAGACGACAGAGGCGGTGGACAGCAGCAAGATGCTGG
			CACCGAACTTTGTTCGCGTGCCGGCCGATCGCGACGCGAGCGAAGGCAAGATGA
			CGCGGTTTGACTGCCGCGTGACGGGCCGACCCTACCCGGACGTGGCCTGGTTC
			ATCAACGGCCAACAGGTGGCTGACGACGCCACGCACAAGATCCTCGTCAACGAG
			TCTGGCAACCACTCGCTCATGATCACCGGCGTCACTCGCTTGGACCACGGAGTG
			GTCGGCTGTATTGC

NL019	SEQ ID NO: 1658	SEQ ID NO: 1659	SEQ ID NO: 1657
	GCGTAATACGACTCA	GAACGCCTGCTCC	GCTTCAGATTTGGGACACGGCCGGCCAGGAGCGGTTCCGCACGATCACATCGAG
	CTATAGGGCTTCAGA	ACATTGG	CTACTACCGGGGCGCCCACGGCATCATTGTGGTGTACGACTGCACCGACCAGGA
	TTTGGGACACGGC	SEQ ID NO: 1661	GTCGTTCAACAACCTCAAACAGTGGCTCGAGGAGATTGACCGCTACGCCTGTGAT
	SEQ ID NO: 1660	GCGTAATACGACT	AATGTCAACAAACTGCTCGTCGGCAACAAGTGTGATCAGACCAACAAAAAGGTCG
	GCTTCAGATTTGGGA	CACTATAGGGAAC	TCGACTATACACAGGCTAAGGAATACGCCGACCAGCTGGGCATTCCGTTCCTGGA
	CACGGC	GCCTGCTCCACAT	GACGTCGGCGAAGAACGCGACCAATGTGGAGCAGGCGTTC
		TGG

NL021	SEQ ID NO: 1663	SEQ ID NO: 1664	SEQ ID NO: 1662
	GCGTAATACGACTCA	CTTCTAGTTCATCC	CGTCAGTCTCAATTCTGTCACCGATATCAGCACCACGTTCATTCTCAAGCCACAAG
	CTATAGGCGTCAGTC	AGGTCGCG	AGAACGTGAAGATAACGCTTGAGGGCGCACAGGCCTGTTTCATTTCACACGAACG
	TCAATTCTGTCACCG	SEQ ID NO: 1666	ACTTGTGATCTCACTGAAGGGAGGAGAACTCTATGTTCTAACTCTCTATTCCGATA
	SEQ ID NO: 1665	GCGTAATACGACT	GTATGCGCAGTGTGAGGAGTTTTCATCTGGAGAAAGCTGCTGCCAGTGTCTTGAC
	CGTCAGTCTCAATTCT	CACTATAGGCTTCT	TACTTGTATCTGTGTTTGTGAGGAGAACTATCTGTTCCTTGGTTCCCGTCTTGGAA
	GTCACCG	AGTTCATCCAGGT	ACTCACTGTTGCTCAGGTTTACTGAGAAGGAATTGAACCTGATTGAGCCGAGGGC
		CGCG	CATCGAAAGCTCACAGTCCCAGAATCCGGCCAAGAAGAAAAAGCTGGATACTTTG
			GGAGATTGGATGGCATCTGACGTCACTGAAATACGCGACCTGGATGAACTAGAAG
			GGTGCTGAGAGCATATTCGGCGGCTACCTGCTGGGAGTTTGTTCGTTGTCTGGAC
			TGGCGCTGTACGACTGGGAGACCCTGGAGCTGGTGCGTCGCATCGAGATCCAAC
			CGAAACACGTGTACTGGTCGGAGAGTGGGGAGCTGGTGGCGCTGGCCACTGAT
			GACTCCTACTTTGTGCTCCGCTACGACGCACAGGCCGTGCTCGCTGCACGCGAC
			GCCGGTGACGACGCTGTCACGCCGGACGGCGTCGAGGATGCATTCGAGGTCCTT
			GGTGAAGTGCACGAAACTGTAAAAACTGGATTG

NL022	SEQ ID NO: 1668	SEQ ID NO: 1669	SEQ ID NO: 1667
	GCGTAATACGACTCA	CAGACGGAAGCAC	CTCACGAGAGGACGTTGCACACTGATATACTGTTCGGTTTGGTGAAAGATGTCGC
	CTATAGGCTCACGAG	TTGCCG	CCGATTCAGACCTGACTTGAAGCTGCTCATATCAAGCGCCACACTGGATGCTCAG
	AGGACGTTGCACAC	SEQ ID NO: 1671	AAATTCTCCGAGTTTTTCGACGATGCACCCATCTTCAGGATTCCGGGCCGTAGATT
	SEQ ID NO: 1670	GCGTAATACGACT	TCCGGTGGACATCTACTACACAAAGGCGCCCGAGGCTGACTACGTGGACGCATG
	CTCACGAGAGGACGT	CACTATAGGCAGA	TGTCGTTTCGATCCTGCAGATCCACGCCACTCAGCCGCTGGGAGACATCCTGGTC
	TGCACAC	CGGAAGCACTTGC	TTCCTCACCGGTCAGGAGGAGATCGAAACCTGCCAGGAGCTGCTGCAGGACAGA
		CG	GTGCGCAGGCTTGGGTCTCGTATCAAGGAGCTGCTCATATTGCCCGTCTATTCCA
			ACCTACCCAGTGATATGCAGGCAAAGATTTTCCTGCCCACTCCACCAAATGCTAG
			AAAGGTAGTATTGGCCACAAATATTGCAGAAACCTCATTGACCATCGACAATATAA
			TCTACGTGATTGATCCTGGTTTTTGTAAGCAGAATAACTTCAATTCAAGGACTGGA
			ATGGAATCGCTTGTTGTAGTGCCTGTTTCAAAGGCATCGGCCAATCAGCGAGCAG
			GGCGGGCGGGACGGGTGGCGGCCGGCAAGTGCTTCCGTCTG

NL023	SEQ ID NO: 1673	SEQ ID NO: 1674	SEQ ID NO: 1672
	GCGTAATACGACTCA	GCAATGTTGTCCTT	GTCCTCGGACGGGAGGTCCACGTGTTTACCGGGATTCCGTTTGCGAAACCTCCC
	CTATAGGGTCCTCGG	GAGCCAGC	ATCGGTCCGTTGCGATTCCGTAAACCGGTTCCCGTCGACCCGTGGCACGGCGTT
	ACGGGAGGTCC	SEQ ID NO: 1676	CTGGATGCGACCGCGCTTCCCAACAGCTGCTACCAGGAACGGTACGAGTATTTC
	SEQ ID NO: 1675	GCGTAATACGACT	CCGGGCTTCGAGGGAGAGGAAATGTGGAATCCGAATACGAATTTGTCCGAAGATT
	GTCCTCGGACGGGAG	CACTATAGGGCAA	GTCTGTATTTGAACATATGGGTGCCGCACCGGTTGAGAATCCGACACAGAGCCAA
	GTCC	TGTTGTCCTTGAG	CAGCGAGGAGAATAAACCAAGAGCGAAGGTGCCGGTGCTGATCTGGATCTACGG
		CCAGC	CGGGGGTTACATGAGCGGCACAGCTACACTGGACGTGTACGATGCTGACATGGT
			GGCCGCCACGAGTGACGTCATCGTCGCCTCCATGCAGTACCGAGTGGGTGCGTT
			CGGCTTCCTCTACCTCGCACAGGACTTGCCTCGAGGCAGCGAGGAGGCGCCGG
			GCAACATGGGGCTCTGGGACCAGGCCCTTGCCATCCGCTGGCTCAAGGACAACA
			TTGC

NL027	SEQ ID NO: 1678	SEQ ID NO: 1679	SEQ ID NO: 1677
	GCGTAATACGACTCA	CAATCCAGTTTTTA	AGAAGACGGCACGGTGCGTATTTGGCACTCGGGCACCTACAGGCTGGAGTCCTC
	CTATAGGAGAAGACG	CAGTTTCGTGC	GCTGAATTATGGCCTCGAAAGAGTGTGGACCATTTGCTGCATGCGAGGATCCAAC
	GCACGGTGCG	SEQ ID NO: 1681	AATGTGGCTCTTGGCTACGACGAAGGCAGCATAATGGTGAAGGTGGGTCGGGAG
	SEQ ID NO: 1680	GCGTAATACGACT	GAGCCGGCCATCTCGATGGATGTGAACGGTGAGAAGATTGTGTGGGCGCGCCAC
	AGAAGACGGCACGGT	CACTATAGGCAAT	TCGGAGATACAACAGGTCAACCTCAAGGCCATGCCGGAGGGCGTCGAAATCAAA
	GCG	CCAGTTTTTACAGT	GATGGCGAACGACTGCCGGTCGCCGTTAAGGATATGGGCAGCTGTGAAATATAT
		TTCGTGC	CCGCAGACCATCGCTCATAATCCCAACGGCAGATTCCTAGTCGTTTGTGGAGATG
			GAGAGTACATAATTCACACATCAATGGTGCTAAGAAATAAGGCGTTTGGCTCGGC
			CCAAGAGTTCATTTGGGGACAGGACTCGTCCGAGTATGCTATCAGAGAAGGAACA
			TCCACTGTCAAAGTATTCAAAAACTTCAAAGAAAAGAAATCATTCAAGCCAGAATTT

TABLE 8-CS

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

CS001	SEQ ID NO: 2041	SEQ ID NO: 2042	SEQ ID NO: 2040
	TAAAGCATGGATGTT	GCGTAATACGACTC	TAAAGCATGGATGTTGGACAAACTGGGTGGCGTGTACGCGCCGCGGCCGTCGAC
	GGACAAACTGGG	ACTATAGGGGTGAG	CGGCCCCCACAAGTTGCGCGAGTGCCTGCCGCTGGTGATCTTCCTCAGGAACCG
	SEQ ID NO: 2043	TCGCACGCCCTTGCC	GCTCAAGTACGCGCTCACCGGAAATGAAGTGCTTAAGATTGTAAAGCAGCGACTT
	GCGTAATACGACTC	SEQ ID NO: 2044	ATCAAAGTTGACGGCAAAGTCAGGACAGACCCCACATATCCCGCTGGATTTATGG
	ACTATAGGTAAAGC	GGTGAGTCGCACGC	ATGTTGTTTCCATTGAAAAGACAAATGAGCTGTTCCGTCTTATATATGATGTCAAAG
	ATGGATGTTGGACA	CCTTGCC	GCAGATTTACTATTCACCGTATTACTCCTGAGGAGGCTAAATACAAGCTGTGCAAG
	AACTGGG		GTGCGGCGCGTGGCGACGGGCCCCAAGAACGTGCCTTACCTGGTGACCCACGA
			CGGACGCACCGTGCGATACCCCGACCCACTCATCAAGGTCAACGACTCCATCCA
			GCTCGACATCGCCACCTCCAAGATCATGGACTTCATCAAGTTTGAATCTGGTAAC
			CTATGTATGATCACGGGAGGCCGTAACTTGGGGCGCGTGGGCACCATCGTGTCC
			CGCGAGCGACATCCCGGGTCCTTCGACATCGTGCATATACGGGACTCCACCGGA
			CATACCTTCGCTACCAGATTGAACAACGTGTTCATAATCGGCAAGGGCACGAAGG
			CGTACATCTCGCTGCCGCGCGGCAAGGGCGTGCGACTCACC

CS002	SEQ ID NO: 2046	SEQ ID NO: 2047	SEQ ID NO: 2045
	CAAGAAGGAGGAGA	GCGTAATACGACTC	CAAGAAGGAGGAGAAGGGTCCATCAACACACGAAGCTATACAGAAATTACGCGAA
	AGGGTCCATCAAC	ACTATAGGCTTGTCT	ACGGAAGAGTTATTGCAGAAGAAACAAGAGTTTCTAGAGCGAAAGATCGACACTG
	SEQ ID NO: 2048	ACATCGATATCCTTG	AATTACAAACGGCGAGAAAACATGGCACAAAGAATAAGAGAGCTGCCATTGCGGC
	GCGTAATACGACTC	TGGGC	ACTGAAGCGCAAGAAGCGTTATGAAAAGCAGCTTACCCAGATTGATGGCACGCTT
	ACTATAGGCAAGAA	SEQ ID NO: 2049	ACCCAAATTGAGGCCCAAAGGGAAGCGCTAGAAGGAGCTAACACCAATACACAG
	GGAGGAGAAGGGTC	CTTGTCTACATCGAT	GTGCTTAACACTATGCGAGATGCTGCTACCGCTATGAGACTCGCCCACAAGGATA
	CATCAAC	ATCCTTGTGGGC	TCGATGTAGACAAG

CS003	SEQ ID NO: 2051	SEQ ID NO: 2052	SEQ ID NO: 2050
	TGGTCTCCGCAACA	GCGTAATACGACTC	TGGTCTCCGCAACAAGCGTGAGGTGTGGAGGGTGAAGTACACGCTGGCCAGGAT
	AGCGTGAGG	ACTATAGGCGAACG	CCGTAAGGCTGCCCGTGAGCTGCTCACACTCGAGGAGAAAGACCCTAAGAGGTT
	SEQ ID NO: 2053	GAGACTTCAGCGAG	ATTCGAAGGTAATGCTCTCCTTCGTCGTCTGGTGAGGATCGGTGTGTTGGATGAG
	GCGTAATACGACTC	AAGTCA	AAGCAGATGAAGCTCGATTATGTACTCGGTCTGAAGATTGAGGACTTCTTGGAAC
	ACTATAGGTGGTCT	SEQ ID NO: 2054	GTCGTCTCCAGACTCAGGTGTTCAAGGCTGGTCTAGCTAAGTCTATCCATCATGC
	CCGCAACAAGCGTG	CGAACGGAGACTTC	CCGTATTCTTATCAGACAGAGGCACATCCGTGTCCGCAAGCAAGTTGTGAACATC
	AGG	AGCGAGAAGTCA	CCTTCGTTCATCGTGCGGCTGGACTCTGGCAAGCACATTGACTTCTCGCTGAAGT
			CTCCGTTCG

CS006	SEQ ID NO: 2056	SEQ ID NO: 2057	SEQ ID NO: 2055
	GGATGATGATGGTA	GCGTAATACGACTC	GGATGATGATGGTATAATTGCACCAGGGATTCGTGTATCTGGTGACGATGTAGTC
	TAATTGCACCAGGG	ACTATAGGCGTTAAA	ATTGGAAAAACTATAACTTTGCCAGAAAACGATGATGAGCTGGAAGGAACATCAA
	SEQ ID NO: 2058	TGGTGTAGCATCAC	GACGATACAGTAAGAGAGATGCCTCTACATTCTTGCGAAACAGTGAAACTGGTATT
	GCGTAATACGACTC	CTATTTCACC	GTTGACCAAGTTATGCTTACACTTAACAGCGAAGGATACAAATTTTGTAAAATACG
	ACTATAGGGGATGA	SEQ ID NO: 2059	TGTGAGATCTGTGAGAATCCCACAAATTGGAGACAAATTTGCTTCTCGTCATGGTC
	TGATGGTATAATTGC	CGTTAAATGGTGTA	AAAAAGGGACTTGTGGTATTCAATATAGGCAAGAAGATATGCCTTTCACTTGTGAA
	ACCAGGG	GCATCACCTATTTCA	GGATTGACACCAGATATTATCATCAATCCACATGCTATCCCCTCTCGTATGACAAT
		CC	TGGTCACTTGATTGAATGTATTCAAGGTAAGGTCTCCTCAAATAAAGGTGAAATAG
			GTGATGCTACACCATTTAACG

CS007	SEQ ID NO: 2061	SEQ ID NO: 2062	SEQ ID NO: 2060
	CTTGTTGAAACCAG	GCGTAATACGACTC	CTTGTTGAAACCAGAGATTTTGAGGGCTATCGTCGATTGCGGTTTCGAGCACCCT
	AGATTTTGAGGGC	ACTATAGGCGGCAT	TCAGAAGTTCAACATGAATGTATTCCCCAAGCTGTTTTGGGAATGGATATTCTTTG
	SEQ ID NO: 2063	GTCATAATTGAAGAC	TCAAAGCTAAATCCGGAATGGGAAAAACCGCCGTATTTGTTTTAGCAACACTGCAA
	GCGTAATACGACTC	TATGTTGACTC	CAGCTAGAACCTTCAGAAAACCATGTTTACGTATTAGTAATGTGCCATACAAGGGA
	ACTATAGGCTTGTTG	SEQ ID NO: 2064	ACTCGCTTTCCAAATAAGCAAGGAATATGAGAGGTTCTCTAAATATATGGCTGGTG
	AAACCAGAGATTTTG	CGGCATGTCATAATT	TTAGAGTATCTGTATTCTTTGGTGGGATGCCAATTCAGAAAGATGAAGAAGTATTG
	AGGGC	GAAGACTATGTTGA	AAGACAGCCTGCCCGCACATCGTTGTTGGTACTCCTGGCAGAATATTAGCATTGG
		CTC	TTAACAACAAGAAACTGAATTTAAAACACCTGAAACACTTCATCCTGGATGAATGT
			GACAAAATGCTTGAATCTCTAGACATGAGACGTGATGTGCAGGAAATATTCAGGA
			ACACCCCTCACGGTAAGCAGGTCATGATGTTTTCTGCAACATTGAGTAAGGAGAT
			CAGACCAGTCTGTAAGAAATTTATGCAAGATCCTATGGAAGTTTATGTGGATGATG
			AAGCTAAACTTACATTGCACGGTTTGCAGCAACATTATGTTAAACTCAAGGAAAAT
			GAAAAGAATAAGAAGTTATTTGAACTTTTGGATGTACTGGAGTTCAACCAAGTTGT
			CATATTTGTAAAGTCAGTGCAGCGCTGCATAGCTCTCGCACAGCTGCTGACAGAC
			CAAAACTTCCCAGCTATTGGTATACACCGAAATATGACTCAAGATGAGCGTCTCTC
			CCGCTATCAGCAGTTCAAAGATTTCCAGAAGAGGATCCTTGTTGCGACAAATCTTT
			TTGGACGGGGTATGGACATTGAAAGAGTCAACATAGT CTTCAATTAT
			GACATGCCG

CS009	SEQ ID NO: 2066	SEQ ID NO: 2067	SEQ ID NO: 2065
	ACGTTTCTGCAGCG	GCGTAATACGACTC	ACGTTTCTGCAGCGGCTGGACTCACGGGAGCCCATGTGGCAGCTGGACGAGAGC
	GCTGGACTC	ACTATAGGGATAATT	ATCATCGGCACCAACCCCGGGCTCGGCTTCCGGCCCACGCCGCCAGAGGTCGC
	SEQ ID NO: 2068	CTTATCGTACGCTGT	CAGCAGCGTCATCTGGTATAAAGGCAACGACCCCAACAGCCAACAATTCTGGGTG
	GCGTAATACGACTC	CATATTCCTG	CAAGAAACCTCCAACTTTCTAACCGCGTACAAACGAGACGGTAAGAAAGCAGGAG
	ACTATAGGACGTTTC	SEQ ID NO: 2069	CAGGCCAGAACATCCACAACTGTGATTTCAAACTGCCTCCTCCGGCCGGTAAGGT
	TGCAGCGGCTGGAC	GATAATTCTTATCGT	GTGCGACGTGGACATCAGCGCCTGGAGTCCCTGTGTAGAGGACAAGCACTTTGG
	TC	ACGCTGTCATATTCC	ATACCACAAGTCCACGCCCTGCATCTTCCTCAAACTCAACAAGATCTTCGGCTGG
		TG	AGGCCGCACTTCTACAACAGCTCCGACAGCCTGCCCACTGACATGCCCGACGAC
			TTGAAGGAGCACATCAGGAATATGACAGCGTACGATAAGAATTATC

CS011	SEQ ID NO 2071	SEQ ID NO: 2072	SEQ ID NO: 2070
	CGACACTTGACTGG	GCGTAATACGACTC	CGACACTTGACTGGAGAGTTCGAGAAAAGATATGTCGCCACATTAGGTGTCGAGG
	AGAGTTCGAGA	ACTATAGGCTCTAG	TGCATCCCTTAGTATTCCACACAAATAGAGGCCCTATAAGGTTTAATGTATGGGAT
	SEQ ID NO: 2073	GTTACCATCACCGA	ACTGCTGGCCAAGAAAAGTTTGGTGGTCTCCGAGATGGTTACTATATCCAAGGTC
	GCGTAATACGACTC	TCAACT	AATGTGCCATCATCATGTTCGATGTAACGTCTCGTGTCACCTACAAAAATGTACCC
	ACTATAGGCGACAC	SEQ ID NO: 2074	AACTGGCACAGAGATTTAGTGCGAGTCTGTGAAGGCATTCCAATTGTTCTTTGTG
	TTGACTGGAGAGTT	CTCTAGGTTACCATC	GCAACAAAGTAGATATCAAGGACAGAAAAGTCAAAGCAAAAACTATTGTTTTCCAC
	CGAGA	ACCGATCAACT	AGAAAAAAGAACCTTCAGTATTATGACATCTCTGCCAAGTCAAACTACAATTTCGA
			GAAACCCTTCCTCTGGTTAGCGAGAAAGTTGATCGGTGATGGTAACCTAGAG

CS013	SEQ ID NO: 2076	SEQ ID NO: 2077	SEQ ID NO: 2075
	TGCCGAACAGGTAT	GCGTAATACGACTC	TGCCGAACAGGTATACATCTCGTCTTTGGCCCTGTTGAAGATGTTAAAACACGGG
	ACATCTCGTCTTTGG	ACTATAGGCCACTA	CGCGCCGGTGTTCCAATGGAAGTTATGGGACTTATGTTAGGTGAATTTGTTGATG
	SEQ ID NO: 2078	CAGCTACAGCACGT	ATTACACGGTGCGTGTCATAGACGTATTTGCCATGCCTCAAACTGGCACAGGAGT
	GCGTAATACGACTC	TCAGAC	GTCGGTTGAAGCTGTAGATCCTGTCTTCCAAGCAAAGATGTTGGATATGTTGAAG
	ACTATAGGTGCCGA	SEQ ID NO: 2079	CAAACTGGACGACCTGAGATGGTAGTGGGATGGTACCACTCGCATCCTGGCTTTG
	ACAGGTATACATCTC	CCACTACAGCTACA	GATGTTGGTTATCTGGAGTCGACATTAATACTCAGCAGTCTTTCGAAGCTTTGTCT
	GTCTTTGG	GCACGTTCAGAC	GAACGTGCTGTAGCTGTAGTGG

CS014	SEQ ID NO: 2081	SEQ ID NO: 2082	SEQ ID NO: 2080
	CAGATCAAGCATAT	GCGTAATACGACTC	AGATCAAGCATATGATGGCCTTCATCGAACAAGAGGCTAATGAAAAGGCCGAGGA
	GATGGCCTTCATCGA	ACTATAGGGAACAA	AATCGATGCAAAGGCCGAAGAGGAGTTCAACATTGAAAAAGGCCGCCTGGTGCA
	SEQ ID NO: 2083	TGCGGTACGTATTT	GCAGCAGCGGCTCAAGATCATGGAATACTACGAAAAGAAAGAGAAACAAGTGGAA
	GCGTAATACGACTC	CGGGC	CTCCAGAAAAAGATCCAATCTTCGAACATGCTGAATCAAGCCCGTCTGAAGGTGC
	ACTATAGGCAGATC	SEQ ID NO: 2084	TCAAAGTGCGTGAGGACCACGTACGCAACGTTCTCGACGAGGCTCGCAAGCGCC
	AAGCATATGATGGC	GAACAATGCGGTAC	TGGCTGAGGTGCCCAAAGACGTGAAACTTTACACAGATCTGCTGGTCACGCTCGT
	CTTCATCGA	GTATTTCGGGC	CGTACAAGCCCTATTCCAGCTCATGGAACCCACAGTAACAGTTCGCGTTAGGCAG
			GCGGACGTCTCCTTAGTACAGTCCATATTGGGCAAGGCACAGCAGGATTACAAAG
			CAAAGATCAAGAAGGACGTTCAATTGAAGATCGACACCGAGAATTCCCTGCCCGC
			CGATACTTGTGGCGGAGTGGAACTTATTGCTGCTAGAGGGCGTATTAAGATCAGC
			AACACTCTGGAGTCTCGTCTGGAGCTGATAGCCCAACAACTGTTGCCCGAAATAC
			GTACCGCATTGTTC

CS015	SEQ ID NO: 2086	SEQ ID NO: 2087	SEQ ID NO: 2085
	ATCGTGCTTTCAGA	GCGTAATACGACTC	ATCGTGCTTTCAGACGATAACTGCCCCGATGAGAAGATCCGCATGAACCGCGTCG
	CGATAACTGCCCC	ACTATAGGCCATTAC	TGCGAAACAACTTGCGTGTACGCCTGTCAGACATAGTCTCCATAGCGCCTTGTCC
	SEQ ID NO: 2088	GATCACGTGCGATG	ATCGGTCAAATATGGGAAACGGGTACATATATTGCCCATTGATGATTCTGTCGAG
	GCGTAATACGACTC	ACTTC	GGTTTGACTGGAAATTTATTCGAAGTCTACTTGAAACCATACTTCATGGAAGCTTA
	ACTATAGGATCGTG	SEQ ID NO: 2089	TCGGCCTATCCATCGCGATGACACATTCATGGTTCGCGGGGGCATGAGGGCTGT
	CTTTCAGACGATAAC	CCATTACGATCACG	TGAATTCAAAGTGGTGGAGACTGATCCGTCGCCGTATTGCATCGTCGCTCCCGAC
	TGCCCC	TGCGATGACTTC	ACAGTGATACACTGCGAAGGAGACCCTATCAAACGAGAGGAAGAAGAAGAAGCC
			CTAAACGCCGTAGGGTACGACGACATCGGTGGCTGTCGTAAACAGCTCGCTCAG
			ATCAAAGAGATGGTCGAGTTGCCTCTAAGGCATCCGTCGCTGTTCAAGGCAATTG
			GTGTGAAGCCGCCACGTGGAATCCTCATGTATGGGCCGCCTGGTACCGGCAAAA
			CTCTCATTGCTCGGGCAGTGGCTAATGAAACTGGTGCATTCTTCTTTCTGATCAAC
			GGGCCGGAGATCATGTCCAAACTCGCGGGCGAGTCCGAATCGAACCTTCGCAAG
			GCATTCGAGGAAGCGGACAAGAACTCCCCGGCTATAATCTTCATCGATGAACTGG
			ATGCCATCGCACCAAAGAGGGAGAAGACTCACGGTGAAGTGGAGCGTCGTATTG
			TGTCGCAACTACTTACTCTTATGGATGGAATGAAGAAGTCATCGCACGTGATCGTA
			ATGG

CS016	SEQ ID NO: 2091	SEQ ID NO: 2092	SEQ ID NO: 2090
	AGGATGGAAGCGGG	GCGTAATACGACTC	AGGATGGAAGCGGGGATACGTTTGAGCATCTCCTTGGGGAAGATACGGAGCAGC
	GATACGTTTGAG	ACTATAGGGCACCC	TGCCAGCCGATGTCCAGCGACTCGAATACTGTGCGGTTCTCGTAGTTGCCCTGTG
	SEQ ID NO: 2093	CTGTCTCCGAAGAC	TGATGAAGTTCTTCTCGAACTTGGTGAGGAACTCGAGGTAGAGCAGATCGTCGGG
	GCGTAATACGACTC	ATGTT	TGTCAGGGCTTCCTCACCGACGACAGCCTTCATGGCCTGCACGTCCTTACCGATG
	ACTATAGGAGGATG	SEQ ID NO: 2094	GCGTAGCAGGCGTACAGCTGGTTGGAAACATCAGAGTGGTCCTTGCGGGTCATT
	GAAGCGGGGATACG	GCACCCCTGTCTCC	CCCTCACCGATGGCAGACTTCATGAGACGAGACAGGGAAGGCAGCACGTTTACA
	TTTGAG	GAAGACATGTT	GGCGGGTAGATCTGTCTGTTGTGGAGCTGACGGTCTACGTAGATCTGTCCCTCAG
			TGATGTAGCCCGTTAAATCGGGAATAGGATGGGTGATGTCGTCGTTGGGCATAGT
			CAAGATGGGGATCTGCGTGATGGATCCGTTTCTACCCTCTACACGCCCGGCTCTC
			TCGTAGATGGTGGCCAAATCGGTGTACATGTAACCTGGGAAACCACGTCGTCCG
			GGCACCTCCTCACGGGCGGCGGACACTTCACGCAGAGCCTCCGCGTACGAAGA
			CATGTCAGTCAAGATTACCAGCACGTGTTTCTCACACTGGTAGGCCAAGAACTCA
			GCAGCAGTCAAGGCCAAACGTGGTGTGATGATTCTCTCAATAGTGGGATCGTTGG
			CCAGATTCAAGAACAGGCACACGTTCTCCATGGAGCCGTTCTCCTCGAAGTCCTG
			CTTGAAGAACCGGGCCGTCTCCATGTTCACACCCATGGCGGCGAACACGATGGC
			AAAGTTGTCCTCGTGGTCGTCCAGCACAGATTTGCCGGGGATCTTTACAAGACCG
			GCTTGCCTACAGATCTGGGCGGCAATTTCGTTGTGTGGCAGACCGGCAGCCGAG
			AAAATGGGGATCTTTTGCCCGCGAGCAATGGAGTTCATCACGTCGATAGCGGAGA
			TACCAGTCTGGATCATTTCCTCAGGGTAGATACGGGACCAGGGGTTGATGGGCT
			GTCCCTGGATGTGTCCAAAAAGTCTTCAGCAAGGATTGGGGGACCTTTGTCAATGGG
			TTTTCCAGAGCCGTTGAATACGCGACCCAACATGTCTTCGGAGACAGGGGTGC

CS018	SEQ ID NO: 2096	SEQ ID NO: 2097	SEQ ID NO: 2095
	CGTCCCTGTACCTG	GCGTAATACGACTC	CGTCCCTGTACCTGCTCAGCAATCCCAACAGCAGCAGAGTTACCGCCACGTCAG
	CTCAGCAATCCCA	ACTATAGGCAGCGT	CGAGAGCGTCGAACACAAATCCTACGGCACGCAAGGGTACACCACTTCGGAACA
	SEQ ID NO: 2098	CGAGGCCCCACCTT	GACCAAGCAGACACAGAAGGTGGCGTACACCAACGGTTCCGACTACTCTTCCAC
	GCGTAATACGACTC	SEQ ID NO: 2099	GGACGACTTTAAGGTGGATACGTTCGAATACAGACTCCTCCGAGAAGTTTCGTTC
	ACTATAGGCGTCCC	CAGCGTCGAGGCCC	AGGGAATCCATCACGAAGCGGTACATTGGCGAGACAGACATTCAGATCAGCACG
	TGTACCTGCTCAGC	CACCTT	GAGGTCGACAAGTCTCTCGGTGTGGTGACCCCTCCTAAGATAGCACAAAAGCCTA
	AATCCCA		GGAATTCCAAGCTGCAGGAGGGAGCCGACGCTCAGTTTCAAGTGCAGCTGTCGG
			GTAACCCGCGGCCACGGGTGTCATGGTTCAAGAACGGGCAGAGGATAGTCAACT
			CGAACAAACACGAAATCGTCACGACACATAATCAAACAATACTTAGGGTAAGAAAC
			ACACAAAAGTCTGATACTGGCAACTACACGTTGTTGGCTGAAAATCCTAACGGAT
			GCGTCGTCACATCGGCATACCTGGCCGTGGAGTCGCCTCAAGAAACTTACGGCC
			AAGATCATAAATCACAATACATAATGGACAATCAGCAAACAGCTGTAGAAGAAAGA
			GTAGAAGTTAATGAAAAAGCTCTCGCTCCGCAATTCGTAAGAGTCTGCCAAGACC
			GCGATGTAACGGAGGGGAAAATGACGCGATTCGATTGCCGCGTCACGGGCAGAC
			CTTACCCAGAAGTCACGTGGTTCATTAACGATAGACAAATTCGAGACGATTATWAT
			CATAAGATATTAGTAAACGAATCGTGTAATCATGCACTTATGATTACAAACGTCGAT
			CTCAGTGATAGTGGCGTAGTATCATGTATAGCACGCAACAAGACCGGCGAAACTT
			CGTTTCAGTGTAGGCTGAACGTGATAGAGAAGGAGCAAGTGGTCGCTCCCAAATT
			CGTGGAGCGGTTCAGCACGCTCAACGTGCGCGAGGGCGAGCCCGTGCAGCTGC
			ACGCGCGCGCCGTCGGCACGCCTACGCCACGCATCACATGGCAGAAGGACGGC
			GTTCAAGTTATACCCAATCCAGAGCTACGAATAAATACCGAAGGTGGGGCCTCGA
			CGCTG

TABLE 8-PX

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

PX001	SEQ ID NO: 2340	SEQ ID NO: 2341	SEQ ID NO: 2339
	GCGTAATACGACTC	CTTGCCGATGATGA	CGAGGTGCTGAAGATCGTGAAGCAGCGCCTCATCAAGGTGGACGGCAAGGTCCG
	ACTATAGGCGAGGT	ACACGTTG	CACCGACCCCACCTACCCGGCTGGATTCATGGATGTTGTGTCGATTGAAAAGACC
	GCTGAAGATCGTGA	SEQ ID NO: 2343	AATGAGCTGTTCCGTCTGATCTACGATGTGAAGGGACGCTTCACCATCCACCGCA
	AG	GCGTAATACGACTC	TCACTCCCGAGGAGGCCAAGTACAAGCTGTGCAAGGTGAAGCGCGTGGCGACG
	SEQ ID NO: 2342	ACTATAGGCTTGCC	GGCCCCAAGAACGTGCCGTACATCGTGACGCACAACGGCCGCACGCTGCGCTAC
	CGAGGTGCTGAAGA	GATGATGAACACGT	CCCGACCCGCTCATCAAGGTCAACGACTCCATCCAGCTCGACATCGCCACCTGC
	TCGTGAAG	TG	AAGATCATGGACATCATCAAGTTCGACTCAGGTAACCTGTGCATGATCACGGGAG
			GGCGTAACTTGGGGCGAGTGGGCACCATCGTGTCCCGCGAGAGGCACCCCGGG
			AGCTTCGACATCGTCCACATCAAGGACACCACCGGACACACCTTCGCCACCAGGT
			TGAACAACGTGTTCATCATCGGCAAG

PX009	SEQ ID NO: 2345	SEQ ID NO: 2346	SEQ ID NO: 2344
	GCGTAATACGACTC	TGTTGATCACTATGC	CAGCTACAAGTATTGGGAGAACCAGCTCATTGACTTTTTGTCAGTATACAAGAAGA
	ACTATAGGCAGCTA	CGGTCCT	AGGGTCAGACAGCGGGTGCTGGTCAGAACATCTTCAACTGTGACTTCCGCAACC
	CAAGTATTGGGAGA	SEQ ID NO: 2348	CGCCCCCACACGGCAAGGTGTGCGACGTGGACATCCGCGGCTGGGAGCCCTGC
	ACCAG	GCGTAATACGACTC	ATTGATGAGAACCACTTCTCTTTCCACAAGTCTTCGCCTTGCATCTTCTTGAAGCT
	SEQ ID NO: 2347	ACTATAGGTGTTGAT	GAATAAGATCTACGGCTGGCGTCCAGAGTTCTACAACGACACGGCTAACCTGCCT
	CAGCTACAAGTATT	CACTATGCCGGTCCT	GAAGCCATGCCCGTGGACTTGCAGACCCACATTCGTAACATTACTGCCTTCAACA
	GGGAGAACCAG		GAGACTATGCGAACATGGTGTGGGTGTCGTGCCACGGCGAGACGCCGGCGGAC
			AAGGAGAACATCGGGCCGGTGCGCTACCTGCCCTACCCGGGCTTCCCCGGGTAC
			TTCTACCCGTACGAGAACGCCGAGGGGTATCTGAGCCCGCTGGTCGCCGTGCAT
			TTGGAGAGGCCGAGGACCGGCATAGTGATCAACA

PX010	SEQ ID NO: 2350	SEQ ID NO: 2351	SEQ ID NO: 2349
	GCGTAATACGACTC	CTGTATCAATGTACC	ACCAGCACTCTAGTGGACAACGTCGCGTTCGGGTCACCACTGTCGCGCGCAATT
	ACTATAGGACCAGC	GCGGCAC	GGGGCGACGCAGCCGCCAACTTACACCACATATCGGCGGGCTTCGACCAGGAG
	ACTCTAGTGGACAA	SEQ ID NO: 2353	GCGGCGGCGGTGGTGATGGCGCGGCTGGTGGTGTACCGCGCGGAGCAGGAGG
	CGTC	GCGTAATACGACTC	ACGGGCCCGACGTGCTGCGCTGGCTCGACCGCATGCTCATACGCCTGTGCCAGA
	SEQ ID NO: 2352	ACTATAGGCTGTATC	AGTTCGGCGAGTACGCGAAGGACGACCCGAACAGCTTCCGTCTGTCGGAGAACT
	ACCAGCACTCTAGT	AATGTACCGCGGCAC	TCAGCCTGTACCCGCAGTTCATGTACCACCTGCGCCGCTCGCAGTTCCTGCAGGT
	GGACAACGTC		CTTCAACAACTCGCCCGACGAGACCACCTTCTACAGACACATGCTGATGCGCGAA
			GACCTGACCCAATCCCTCATCATGATCCAGCCGATCCTCTACTCGTACAGCTTCG
			GAGGCGCGCCCGAACCCGTGCTGTTAGACACCAGCTCCATCCAGCCCGACCGCA
			TCCTGCTCATGGACACCTTCTTCCAGATCCTCATCTACCATGGAGAGACAATGGC
			GCAATGGCGCGCTCTCCGCTACCAAGACATGGCTGAGTACGAGAACTTCAAGCA
			GCTGCTGCGAGCGCCCGTGGACGACGCGCAGGAGATCCTGCAGACCAGGTTCC
			CCGTGCCGCGGTACATTGATACAG

PX015	SEQ ID NO: 2355	SEQ ID NO: 2356	SEQ ID NO: 2354
	GCGTAATACGACTC	GATGATGGCCGGAG	GACGAGAAGATCCGCATGAACCGCGTCGTCCGGAACAACCTGCGAGTGCGCCTG
	ACTATAGGGACGAG	AGTTCTTG	TCAGACATTGTGTCCATCGCTCCTTGCCCGTCAGTGAAGTACGGCAAGAGAGTTC
	AAGATCCGCATGAA	SEQ ID NO: 2358	ATATTCTGCCCATTGATGACTCTGTTGAGGGTTTGACTGGAAACCTGTTCGAAGTC
	CC	GCGTAATACGACTC	TACCTGAAGCCGTACTTCATGGAGGCGTACCGGCCCATCCACCGCGACGACACG
	SEQ ID NO: 2357	ACTATAGGGATGAT	TTCATGGTGCGCGGCGGCATGCGCGCCGTCGAGTTCAAGGTGGTGGAGACCGA
	GACGAGAAGATCCG	GGCCGGAGAGTTCT	CCCCTCGCCCTACTGCATCGTGGCCCCCGACACGGTCATTCATTGTGAGGGAGA
	CATGAACC	TG	GCCGATTAAACGCGAGGAAGAAGAGGAGGCTCTCAACGCCGTCGGCTACGACGA
			CATCGGCGGGTGCCGCAAGCAGCTGGCGCAGATCAAGGAGATGGTGGAGCTGC
			CGCTGCGCCACCCCTCGCTGTTCAAGGCCATCGGGGTCAAGCCGCCGCGGGGG
			ATACTGATGTACGGGCCCCCGGGGACGGGGAAGACCTTGATCGCTAGGGCTGTC
			GCTAATGAGACGGGCGCATTCTTCTTCCTCATCAACGGCCCCGAGATCATGTCGA
			AACTCGCCGGTGAATCCGAGTCGAACCTGCGCAAGGCGTTCGAGGAGGCGGACA
			AGAACTCTCCGGCCATCATC

PX016	SEQ ID NO: 2360	SEQ ID NO: 2361	SEQ ID NO: 2359
	GCGTAATACGACTC	AGTGATGTACCCGG	CTGGGTCGTATTTTCAACGGCTCCGGCAAGCCCATCGACAAGGGGCCCCCGATC
	ACTATAGGCTGGGT	TCAAGTCG	CTGGCCGAGGAGTACCTGGACATCCAGGGGCAGCCCATCAACCCGTGGTCCCGT
	CGTATTTTCAACGG	SEQ ID NO: 2363	ATCTACCCGGAGGAGATGATCCAGACTGGTATCTCCGCTATCGACGTGATGAACT
	CTC	GCGTAATACGACTC	CCATCGCCCGTGGTCAGAAGATCCCCATCTTCTCCGCCGCCGGTCTGCCCCACA
	SEQ ID NO: 2362	ACTATAGGAGTGAT	ACGAGATTGCTGCTCAGATCTGTAGGCAGGCTGGTCTTGTCAAGGTCCCCGGAAA
	CTGGGTCGTATTTTC	GTACCCGGTCAAGT	ATCCGTGTTGGACGACCACGAAGACAACTTCGCCATCGTGTTCGCCGCCATGGG
	AACGGCTC	CG	AGTCAACATGGAGACCGCCAGGTTCTTCAAGCAGGACTTCGAGGAGAACGGTTC
			CATGGAGAACGTCTGTCTGTTCTTGAACTTGGCCAATGACCCGACCATTGAGAGG
			ATTATCACGCCGAGGTTGGCGCTGACTGCTGCCGAGTTCTTGGCCTACCAGTGC
			GAGAAACACGTGTTGGTAATCTTGACCGACATGTCTTCATACGCGGAGGCTCTTC
			GTGAAGTGTCAGCCGCCCGTGAGGAGGTGCCCGGACGACGTGGTTTCCCAGGTT
			ACATGTACACGGATTTGGCCACAATCTACGAGCGCGCCGGGCGAGTCGAGGGCC
			GCAACGGCTCCATCACGCAGATCCCCATCCTGACCATGCCCAACGACGACATCA
			CCCACCCCATCCCCGACTTGACCGGGTACATCACT

TABLE 8-AD

Target	Primers Forward	Primers Reverse	dsRNA DNA Sequence (sense strand)
ID	5′ → 3′	5′ → 3′	5′ → 3′

AD001	SEQ ID NO: 2462	SEQ ID NO: 2463	SEQ ID NO: 2461
	GCGTAATACGACTC	CAATATCAAACGAG	GCTCCTAAAGCATGGATGTTGGACAAACTCGGAGGAGTATTCGCTCCTCGCCCCAG
	ACTATAGGGCTCCT	CCTGGGTG	TACTGGCCCCCACAAATTGCGTGAATGTTTACCTTTGGTGATTTTTCTTCGCAATCG
	AAAGCATGGATGTT	SEQ ID NO: 2465	GCTCAAGTATGCTCTGACGAACTGTGAAGTAACGAAGATTGTTATGCAGCGACTTAT
	GG	GCGTAATACGACTC	CAAAGTTGACGGCAAGGTGCGAACCGATCCGAATTATCCCGCTGGTTTCATGGATG
	SEQ ID NO: 2464	ACTATAGGCAATATC	TTGTCACCATTGAGAAGACTGGAGAGTTCTTCAGGCTGGTGTATGATGTGAAAGGC
	GCTCCTAAAGCATG	AAACGAGCCTGGGTG	CGTTTCACAATTCACAGAATTAGTGCAGAAGAAGCCAAGTACAAGCTCTGCAAGGTC
	GATGTTGG		AGGAGAGTTCAAACTGGGCCAAAAGGTATTCCATTCTTGGTGACCCATGATGGCCG
			TACTATCCGTTATCCTGACCCAGTCATTAAAGTTAATGACTCAATCCAATTGGATATT
			GCCACTTGTAAAATCATGGACCACATCAGATTTGAATCTGGCAACCTGTGTATGATT
			ACTGGTGGACGTAACTTGGGTCGAGTGGGGACTGTTGTGAGTCGAGAACGTCACC
			CAGGCTCGTTTGATATTG

AD002	SEQ ID NO: 2467	SEQ ID NO: 2468	SEQ ID NO: 2466
	GCGTAATACGACTC	CATCCATGTGCTGA	GAAGAAAGATGGAAAGGCTCCGACCACTGGTGAGGCCATTCAGAAACTCAGAGAAA
	ACTATAGGGAAGAA	TGAGCTGC	CAGAAGAAATGTTAATCAAAAAGCAGGAATTTTTAGAGAAGAAAATCGAACAAGAAA
	AGATGGAAAGGCTC	SEQ ID NO: 2470	TCAATGTTGCAAAGAAAAATGGAACGAAAAATAAGCGAGCTGCTATTCAGGCTCTGA
	CGAC	GCGTAATACGACTC	AAAGGAAAAAGAGGTATGAAAAACAATTGCAGCAAATTGATGGCACCTTATCCACAA
	SEQ ID NO: 2469	ACTATAGGCATCCAT	TTGAAATGCAAAGAGAAGCTTTGGAGGGTGCTAATACTAATACAGCTGTATTACAAA
	GAAGAAAGATGGAA	GTGCTGATGAGCTGC	CAATGAAATCAGCAGCAGATGCCCTTAAAGCAGCTCATCAGCACATGGATG
	AGGCTCCGAC

AD009	SEQ ID NO: 2472	SEQ ID NO: 2473	SEQ ID NO: 2471
	GCGTAATACGACTC	CGTGTTCATCTCCCT	GTCTTCTTCCAGACACTGGATCCTCGTATTCCCACCTGGCAGTTAGATTCTTCTATC
	ACTATAGGGTCTTCT	CGAGTTG	ATTGGCACATCACCTGGCCTAGGTTTCCGGCCAATGCCAGAAGATAGCAATGTAGA
	TCCAGACACTGGAT	SEQ ID NO: 2475	GTCAACTCTCATCTGGTACCGTGGAACAGATCGTGATGACTTCCGTCAGTGGACAG
	CCTC	GCGTAATACGACTC	ACACCCTTGATGAATTTCTTGCTGTGTACAAGACTCCTGGTCTGACCCCTGGTCGAG
	SEQ ID NO: 2474	ACTATAGGCGTGTT	GTCAGAACATCCACAACTGTGACTATGATAAGCCGCCAAAGAAAGGCCAAGTTTGC
	GTCTTCTTCCAGACA	CATCTCCCTCGAGT	AATGTGGACATCAAGAATTGGCATCCCTGCATrCAAGAGAATCACTACAACTACCAC
	CTGGATCCTC	TG	AAGAGCTCTCCATGCATATTCATCAAGCTCAACAAGATCTACAATTGGATCCCTGAA
			TACTACAATGAGAGTACGAATTTGCCTGAGCAGATGCCAGAAGACCTGAAGCAGTA
			CATCCACAACCTGGAGAGTAACAACTCGAGGGAGATGAACACG

AD015	SEQ ID NO: 2477	SEQ ID NO: 2478	SEQ ID NO: 2476
	GCGTAATACGACTC	AGAATTTCAAGGCG	GTTGAAGGACTAACCGGGAATTTGTTTGAGGTGTACTTAAAACCGTACTTTCTCGAA
	ACTATAGGGTTGAA	ACCAGTGG	GCATACCGACCCATTCACAAAGATGATGCGTTTATTGTTCGTGGTGGTATGCGAGCA
	GGACTAACCGGGAA	SEQ ID NO: 2480	GTAGAATTCAAAGTAGTGGAAACAGATCCTTCACCATATTGTATTGTTGCTCCTGATA
	TTTG	GCGTAATACGACTC	CTGTTATTCACTGTGAAGGTGATCCAATAAAACGTGAAGAGGAAGAAGAAGCATTAA
	SEQ ID NO: 2479	ACTATAGGAGAATTT	ATGCTGTTGGTTATGATGACATTGGGGGTTGCCGAAAACAGCTAGCACAGATCAAG
	GTTGAAGGACTAAC	CAAGGCGACCAGTGG	GAAATGGTGGAATTGCCATTACGGCACCCCAGTCTCTTTAAGGCTATTGGTGTTAAG
	CGGGAATTTG		CCACCGAGGGGAATACTGCTGTATGGACCCCCTGGAACTGGTAAAACCCTCATTGC
			CAGGGCTGTGGCTAATGAAACTGGTGCATTCTTCTTTTTAATAAATGGTCCTGAAATT
			ATGAGCAAGCTTGCTGGTGAATCTGAAAGCAACTTACGTAAGGCATTTGAAGAAGCT
			GATAAGAATGCTCCGGCAATTATATTTATTGATGAACTAGATGCAATTGCCCCTAAAA
			GAGAAAAAACTCATGGAGAGGTGGAACGTCGCATAGTTTCACAACTACTAACTTTAA
			TGGATGGTCTGAAGCAAAGTTCACATGTTATTGTTATGGCTGCCACAAATAGACCCA
			ACTCTATTGATGGTGCCTTGCGCCGCTTTGGCAGATTTGATAGGGAAAGATATTG
			GTATACCAGATGCCACTGGTCGCCTTGAAATTCT

AD016	SEQ ID NO: 2482	SEQ ID NO: 2483	SEQ ID NO: 2481
	GCGTAATACGACTC	ATGTAGCCTGGGAA	ACCCGGAAGAAATGATCCAGACGGGGATCTCGACCATCGACGTGATGACGTCCATC
	ACTATAGGACCCGG	GCCTCTTC	GCGCGAGGGCAGAAGATCCCCATCTTCTCGGGCGCAGGGCTGCCACACAACGAGA
	AAGAAATGATCCAG	SEQ ID NO: 2485	TCGCTGCGCAGATCTGCCGACAGGCGGGGCTGGTGCAGCACAAGGAGAACAAGGA
	AC	GCGTAATACGACTC	CGACTTCGCCATCGTGTTCGCGGCGATGGGCGTCAACATGGAGACGGCGCGCTTC
	SEQ ID NO: 2484	ACTATAGGATGTAG	TTCAAGCGCGAGTTCGCGCAGACGGGCGCGTGCAACGTGGTGCTGTTCCTCAACC
	ACCCGGAAGAAATG	CCTGGGAAGCCTCT	TGGCCAACGACCCCACCATCGAGCGCATCATCACCCCGCGCCTCGCGCTCACCGT
	ATCCAGAC	TC	GGCCGAGTTCCTGGCCTACCAGTGCAACAAGCACGTGCTCGTCATCATGACCGACA
			TGACCTCCTACGCGGAGGCGCTGCGCGAGGTGAGCGCGGCGCGCGAGGAGGTTC
			CTGGGCGAAGAGGCTTCCCAGGCTACAT

TABLE 9-LD

	Hairpin Sequence
Target ID
	5′ → 3′

LD002	SEQIDNO: 240
	GCCCTTGCAATGTCATCCATCATGTCGTGTACATTGTCCACGTCCAAGTTTATGGGCTTTCTTAAGAGCTTCAGCTGCATTTTTCAT
	AGATTCCAATACTGTGGTGTTCGTACTAGCTCCCTCCAGAGCTTCTCGTTGAAGTTCAATAGTAGTTAAAGTGCCATCTATTTGCAACT
	GATTTTTTTCTAATCGCTTCTTCCGCTTCAGCGCTTGCATGGCCGCTCAAGGGCGAATTCACCAGCTTTCTTGTACAAAGTGGTATATC
	ACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGA
	CCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACT
	CATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTC
	TACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAG
	CTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTT
	CAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTT
	TTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATA
	TGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCC
	GGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTTTCCCTAAAGGGTTTATTGAGAATATGTTT
	TTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCAT
	GGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGG
	CAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATA
	AAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAG
	ACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTA
	TGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCA
	TAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGA
	TCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTTGAGCGGCCATGCAAGCGCTGAAGCGGAAGAAGCG
	ATTAGAAAAAAATCAGTTGCAAATAGATGGCACTTTAACTACTATTGAACTTCAACGAGAAGCTCTGGAGGGAGCTAGTACGAACACC
	ACAGTATTGGAATCTATGAAAAATGCAGCTGAAGCTCTTAAGAAAGCCCATAAAAACTTGGACGTGGACAATGTACACGACATGATGG
	ATGACATTGCAAGGGC

LD006	SEQIDNO: 241
	GCCCTTGGAGCGAGACTACAACAACTATGGCTGGCAGGTGTTGGTTGCTTCTGGTGTGGTGGAATACATCGACACTCTTGAAGAAGA
	AACTGTCATGATTGCGATGAATCCTGAGGATCTTCGGCAGGACAAAGAATATGCTTATTGTACGACCTACACCCACTGCGAAATCCAC
	CCGGCCATGATCTTGGGCGTTTGCGCGTCTATTATACCTTTCCCCGATCATAACCAGAGCCCAAGGAACACCTACCAGAGCGCTATG
	GGTAAGCAAGCTATGGGGGTCTACATTACGAATTTCCACGTGCGGATGGACACCCTGGCCCACGTGCTATACTACCCGCACAAACCT
	CTGGTCACTACCAGGTCTATGGAGTATCTGCGGTTCAGAGAATTACCAGCCGGGATCAACAGTATAGTTGCTATTGCTTGTTATACTG
	GTTATAATCAAGAAGATTCTGTTATTCTGAACGCGTCTGCTGTGGAAAGAGGATTTTTCCGATCCGTGTTTTATCGTTCCTATAAAGAT
	GCCGAATCGAAGCGAATTGGCGATCAAGAAGAGCAGTTCGAGAAGGGCGAATTCACCAGCTTTCTTGTACAAAGTGGTATATCACTA
	GTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTGAAGCTGACCTG
	CAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATT
	AACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACA
	ATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAA
	GGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGT
	CAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTAT
	CCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGG
	GATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGC
	AGTTTCTACACATATATTCGGAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTC
	GTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGG
	GCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCA
	GAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAA
	GTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGAC
	GATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATG
	CTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATA
	GAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATC
	CCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGGCCCTTCTCGAACTGCTCTTCTTGATCGCCAATTCGCTTCGATTCGGC
	ATCTTTATAGGAACGATAAAACACGGATCGGAAAAATCCTCTTTCCACAGCAGACGCGTTCAGAATAACAGAATCTTCTTGATTATAAC
	CAGTATAACAAGCAATAGCAACTATACTGTTGATCCCGGCTGGTAATTCTCTGAACCGCAGATACTCCATAGACCTGGTAGTGACCAG
	AGGTTTGTGCGGGTAGTATAGCACGTGGGCCAGGGTGTCCATCCGCACGTGGAAATTCGTAATGTAGACCCCCATAGCTTGCTTACC
	CATAGCGCTCTGGTAGGTGTTCCTTGGGCTCTGGTTATGATCGGGGAAAGGTATAATAGACGCGCAAACGCCCAAGATCATGGCCG
	GGTGGATTTCGCAGTGGGTGTAGGTCGTACAATAAGCATATTCTTTGTCCTGCCGAAGATCCTCAGGATTCATCGCAATCATGACAGT
	TTCTTCTTCAAGAGTGTCGATGTATTCCACCACACCAGAAGCAACCAACACCTGCCAGCCATAGTTGTTGTAGTCTCGCTCCAAGGGC

LD007	SEQIDNO: 242
	GCCCTTCCGAAGAAGGATGTGAAGGGTACTTACGTATCCATACACAGTTCAGGCTTCAGAGATTTTTTATTGAAACCAGAAATTCTAA
	GAGCTATAGTTGACTGCGGTTTTGAACACCCTTCAGAAGTTCAGCACGAATGTATTCCTCAAGCTGTCATTGGCATGGACATTTTATGT
	CAAGCCAAATCTGGTATGGGCAAAACGGCAGTGTTTGTTCTGGCGACACTGCAACAATTGGAACCAGCGGACAATGTTGTTTACGTTT
	TGGTGATGTGTCACACTCGTGAACTGGCTTTCCAAATCAGCAAAGAGTACGAGAGGTTCAGTAAATATATGCCCAGTGTCAAGGTGG
	GCGTCTTTTTCGGAGGAATGCCTATTGCTAACGATGAAGAAGTATTGAAAAACAAATGTCCACACATTGTTGTGGGGACGCCTGGGC
	GTATTTTGGCGCTTGTCAAGTCTAGGAAGCTAGTCCTCAAGAACCTGAAACACTTCATTCTTGATGAGTGCGATAAAATGTTAGAACTG
	TTGGATATGAGGAGAGACGTCCAGGAAATCTACAGAAACACCCCTCACACCAAGCAAGTGATGATGTTCAGTGCCACACTCAGCAAA
	GAAATCAGGCCGGTGTGCAAGAAATTCATGCAAGATCCAATGGAGGTGTATGTAGACGATGAAGCCAAATTGACGTTGCACGGATTA
	CAACAGCATTACGTTAAACTCAAAGAAAATGAAAAGAATAAAAAATTATTTGAGTTGCTCGATGTTCTCGAATTTAATCAGGTGGTCATT
	TTTGTGAAGTCCGTTCAAAGGTGTGTGGCTTTGGCACAGTTGCTGACTGAACAGAATTTCCCAGCCATAGGAATTCACAGAGGAATG
	GACCAGAAAGAGAGGTTGTCTCGGTATGAGCAGTTCAAAGATTTCCAGAAGAGAATATTGGTAGCTACGAATCTCTTTGGGCGTGGC
	ATGGACATTGAAAGGGTCAACATTGTCTTCAACTATGATATGCCAGAGGACTCCGACACCTACTTGCATCGAAGGGCGAATTCACCAG
	CTTTCTTGTACAAAGTGGTATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGC
	TGTTATGTTCAGTGCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAG
	CTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGC
	TAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCT
	GATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATC
	GTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACC
	GTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAAT
	GAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGG
	AGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTA
	AAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAAC
	TTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCC
	GTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGG
	ATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTC
	TAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATAC
	AAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAG
	CATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCA
	TCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCGACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTTCGATGCAAGTA
	GGTGTCGGAGTCCTCTGGCATATCATAGTTGAAGACAATGTTGACCCTTTCAATGTCCATGCCACGCCCAAAGAGATTCGTAGCTACC
	AATATTCTCTTCTGGAAATCTTTGAACTGCTCATACCGAGACAACCTCTCTTTCTGGTCCATTCCTCTGTGAATTCCTATGGCTGGGAA
	ATTCTGTTCAGTCAGCAACTGTGCCAAAGCCACACACCTTTGAACGGACTTCACAAAAATGACCACCTGATTAAATTCGAGAACATCG
	AGCAACTCAAATAATTTTTTATTCTTTTCATTTTCTTTGAGTTTAACGTAATGCTGTTGTAATCCGTGCAACGTCAATTTGGCTTCATCGT
	CTACATACACCTCCATTGGATCTTGCATGAATTTCTTGCACACCGGCCTGATTTCTTTGCTGAGTGTGGCACTGAACATCATCACTTGC
	TTGGTGTGAGGGGTGTTTCTGTAGATTTCCTGGACGTCTCTCCTCATATCCAACAGTTCTAACATTTTATCGCACTCATCAAGAATGAA
	GTGTTTCAGGTTCTTGAGGACTAGCTTCCTAGACTTGACAAGCGCCAAAATACGCCCAGGCGTCCCCACAACAATGTGTGGACATTT
	GTTTTTCAATACTTCTTCATCGTTAGCAATAGGCATTCCTCCGAAAAAGACGCCCACCTTGACACTGGGCATATATTTACTGAACCTCT
	CGTACTCTTTGCTGATTTGGAAAGCCAGTTCACGAGTGTGACACATCACCAAAACGTAAACAACATTGTCCGCTGGTTCCAATTGTTG
	CAGTGTCGCCAGAACAAACACTGCCGTTTTGCCCATACCAGATTTGGCTTGACATAAAATGTCCATGCCAATGACAGCTTGAGGAATA
	CATTCGTGCTGAACTTCTGAAGGGTGTTCAAAACCGCAGTCAACTATAGCTCTTAGAATTTCTGGTTTCAATAAAAAATCTCTGAAGCC
	TGAACTGTGTATGGATACGTAAGTACCCTTCACATCCTTCTTCGGAAGGGC

LD010	SEQIDNO: 243
	GCCCTTCGCCATTGGGCGATGGTTTCGCCATGGAATATCAGAATCTGGAAGAACGTGTCCATGAGCAGAATTCTATCGGGTTGGATG
	GAACTCGTATCCAAAAGCACAGGTTCTGGTGGTCCATTGAAACTGTAGCTGTAGAGTATCGGCTGGATCATGATCAGCGACTGCGTG
	AGGTCTTCGCGCATAAGCATGTGCCTGTAGAAGGACGTTTCGTCGGGAGAATTGTTAAACACCTGCAGGAACTGTGACCTTCTCAAA
	TGGTACATGAACTGCGGGTAGAGGCTGAAGTTTTCGCCCAAGCGGAACGAATTCGGGTCGTCCTTGTTATATTCGCCGAATTTCTGG
	CACAGACGTATCAACATCCTATCGACCCATCTCAAAACATCAGGGCTATCGTCTGATTCCGCTCTGTAAACTGCCATCCTCGCCATTA
	TCACTGCGGCTGCCTCCTGATCGAATCCAGCACTGACATGATGTATATTAGCGGAAGCATCGGCCCAGTTTCTAGCAACTGTCGTTA
	CTCGGATCCTCTTCTGGCCACTAGCATGCTGATATTGCGTGATGAACTGTATGCAGCCCCTTCCCCCTTGAGGTATGGGAGCGGAAT
	GTTGGTTGACGACCTCGAAGAACAAGGCCATGGTAGTACTTGGAGTTACCGTACACATTTTCCACTGGACCGTGTTACCCATTCCTAT
	TTCGGTGTCGGAAACCAAAGGATTCTTCACATTCAACGAAACACAAGATCCAATACCGCCTTGAATTTTCAACTCCCTGGAACACTTG
	ACCCTCCAGAGTACCATTAAATGCCATCTTCAGCTCGTTTTCTGATCTTTCGAAAATATGCGCTGGAACGTTTGCTTGAACAGGGAA
	GAATTGAACGAGTCGCCCATGACCATATGTCCCCCTGTTGAATTACAACACTGTTTCATCTCCATCAATCCTGTCTGATCCAAAGCGC
	ATGAATATATGTCAACGCAGTGGCCATTCGTTGCTGCTCTCATCGCTAAATTATCATAGTGCTTGATTGCTTTCTTCATGTATTTGGCAT
	TGTCTTTTTGGATGTCGTGGTGAGATCTGATAGGTTGCTTCAGATCATCATTCAAGACTTGACCAGGGCCTTGAGAGCAAGGTCCTCC
	AACGAATAGCATGACCCTGGCACCAGTATTGGCGTATGTGCACTCCAACAACCCAATGGCTATCGATAAAGCTGTCCCGGTCGATCT
	AAGGGCGCATTTGCCTTGGTGGACAGGCCATGGGTCTCTTTGCAACTCTCCAATAAGATCAGTGAGGTTCATGTCGCATTTCGAGAT
	GGGTTGAAGGAACCTGCTTCCTGGTGGCGTAGGAGCTTGCTGGAGTGCTCCAGGCCTCATGGGTTGTCCTGGTTGTTGAGGAGCAG
	GTTGAGCACTTACTGCGGCTCTGCCCACTTCCAACATCTCTTGAACTTGCTTAGCTGTGAGGTCTTTCGTCCCTCGGAAAACGTAAGA
	TTTGCTGCAGCCCTCGGTACCTAGTTCGTGCACTTGGACCATCTTCCCAAAGGTAATCAACCCTATCAAGGCATTCGGGGGCAACAA
	GCTCAAAGACATCTGCAACGAATCCTTGAGAGAAGGGCGAATTCACCAGCTTTCTTGTACAAAGTGGTATATCACTAGTGCGGCCGC
	CTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTA
	AATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCAC
	CTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAA
	ACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
	GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAAT
	GTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATT
	CACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCAC
	CCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACAT
	ATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATC
	CCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATAC
	GCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGA
	ATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAAC
	AAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTT
	TTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCAT
	AATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTG
	AAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATC
	GACCACTTTGTACAAGAAAGCTGGTCGAATTCGCCCTTCTCTCAAGGATTCGTTGCAGATGTCTTTGAGCTTGTTGCCCCCGAATGCC
	TTGATAGGGTTGATTACCTTTGGGAAGATGGTCCAAGTGCACGAACTAGGTACCGAGGGCTGCAGCAAATCTTACGTTTTCCGAGGG
	ACGAAAGACCTCACAGCTAAGCAAGTTCAAGAGATGTTGGAAGTGGGCAGAGCCGCAGTAAGTGCTCAACCTGCTCCTCAACAACCA
	GGACAACCCATGAGGCCTGGAGCACTCCAGCAAGCTCCTACGCCACCAGGAAGCAGGTTCCTTCAACCCATCTCGAAATGCGACAT
	GAACCTCACTGATCTTATTGGAGAGTTGCAAAGAGACCCATGGCCTGTCCACCAAGGCAAATGCGCCCTTAGATCGACCGGGACAGC
	TTTATCGATAGCCATTGGGTTGTTGGAGTGCACATACGCCAATACTGGTGCCAGGGTCATGCTATTCGTTGGAGGACCTTGCTCTCAA
	GGCCCTGGTCAAGTCTTGAATGATGATCTGAAGCAACCTATCAGATCTCACCACGACATCCAAAAAGACAATGCCAAATACATGAAGA
	AAGCAATCAAGCACTATGATAATTTAGCGATGAGAGCAGCAACGAATGGCCACTGCGTTGACATATATTCATGCGCTTTGGATCAGAC
	AGGATTGATGGAGATGAAACAGTGTTGTAATTCAACAGGGGGACATATGGTCATGGGCGACTCGTTCAATTCTTCCCTGTTCAAGCAA
	ACGTTCCAGCGCATATTTTCGAAAGATCAGAAAAACGAGCTGAAGATGGCATTTAATGGTACTCTGGAGGGTCAAGTGTTCCAGGGA
	GTTGAAAATTCAAGGCGGTATTGGATCTTGTGTTTCGTTGAATGTGAAGAATCCTTTGGTTTCCGACACCGAAATAGGAATGGGTAAC
	ACGGTCCAGTGGAAAATGTGTACGGTAACTCCAAGTACTACCATGGCCTTGTTCTTCGAGGTCGTCAACCAACATTCCGCTCCCATAC
	CTCAAGGGGGAAGGGGCTGCATACAGTTCATCACGCAATATCAGCATGCTAGTGGCCAGAAGAGGATCCGAGTAACGACAGTTGCT
	AGAAACTGGGCCGATGCTTCCGCTAATATACATCATGTCAGTGCTGGATTCGATCAGGAGGCAGCCGCAGTGATAATGGCGAGGATG
	GCAGTTTACAGAGCGGAATCAGACGATAGCCCTGATGTTTTGAGATGGGTCGATAGGATGTTGATACGTCTGTGCCAGAAATTCGGC
	GAATATAACAAGGACGACCCGAATTCGTTCCGCTTGGGCGAAAACTTCAGCCTCTACCCGCAGTTCATGTACCATTTGAGAAGGTCA
	CAGTTCCTGCAGGTGTTTAACAATTCTCCCGACGAAACGTCCTTCTACAGGCACATGCTTATGCGCGAAGACCTCACGCAGTCGCTG
	ATCATGATCCAGCCGATACTCTACAGCTACAGTTTCAATGGACCACCAGAACCTGTGCTTTTGGATACGAGTTCCATCCAACCCGATA
	GAATTCTGCTCATGGACACGTTCTTCCAGATTCTGATATTCCATGGCGAAACCATCGCCCAATGGCGAAGGGC

LD011	SEQ ID NO: 244
	GCCCTTGTGGAAGCAGGGCTGGCATGGCGACAAATTCTAGATTGGGATCACCAATAAGCTTCCTAGCTAGCCATAGGAAAGGCTTCT
	CAAAGTTGTAGTTAGATTTGGCAGAGATATCATAGTACTGCAAATTCTTCTTCCTATGAAAGACAATACTTTTCGCTTTTACTTTTCTGT
	CTTTGATGTCAACCTTGTTCCCGCAAAGTACTATCGGGATATTTTCACAGACTCTGACAAGATCTCTGTGCCAATTTGGTACATTCTTG
	TATGTAACTCTGGAAGTTACATCAAACATGATAATAGCACACTGTCCCTGAATGTAATATCCATCACGGAGACCACCAAACTTCTCCTG
	ACCGGCAGTGTCCCATACATTGAACCGAATAGGGCCCCTGTTTGTATGGAAGACCAGAGGATGGACTTCAACTCCCAAAGTAGCTAC
	ATATCTTTTTTCAAATTCACCAGTCATATGACGTTTCACAAATGTCGTTTTTCCAGTACCTCCATCTCCGACCAACACACACTTGAAAGT
	GGGAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACC
	TGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAG
	ACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTC
	GACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGC
	CATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACC
	ACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCT
	GGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATG
	CTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCA
	AACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTAC
	GGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGA
	TTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCG
	CTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG
	CAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAG
	AAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTA
	AACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGAT
	AAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGG
	CAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCGGACCACTTTGTACAAGAAAGCTGGGT
	CGAATTCGCCCTTCCCACTTTCAAGTGTGTGTTGGTCGGAGATGGAGGTACTGGAAAAACGACATTTGTGAAACGTCATATGACTGGT
	GAATTTGAAAAAAGATATGTAGCTACTTTGGGAGTTGAAGTCCATCCTCTGGTCTTCCATACAAACAGGGGCCCTATTCGGTTCAATG
	TATGGGACACTGCCGGTCAGGAGAAGTTTGGTGGTCTCCGTGATGGATATTACATTCAGGGACAGTGTGCTATTATCATGTTTGATGT
	AACTTCCAGAGTTACATACAAGAATGTACCAAATTGGCACAGAGATCTTGTCAGAGTCTGTGAAAATATCCCGATAGTACTTTGCGGG
	AACAAGGTTGACATCAAAGACAGAAAAGTAAAAGCGAAAAGTATTGTCTTTCATAGGAAGAAGAATTTGCAGTACTATGATATCTCTGC
	CAAATCTAACTACAACTTTGAGAAGCCTTTCCTATGGCTAGCTAGGAAGCTTATTGGTGATCCCAATCTAGAATTTGTCGCCATGCCAG
	CCCTGCTTCCACAAGGGC

LD014	SEQ ID NO: 245
	GCCCTTCGCAGATCAAGCATATGATGGCTTTCATTGAACAAGAGGCAAACGAAAAGGCAGAAGAAATCGATGCCAAGGCCGAGGAAG
	AATTTAATATTGAAAAGGGGCGCCTTGTTCAGCAACAACGTCTCAAGATTATGGAATATTATGAGAAGAAAGAGAAACAGGTCGAACT
	CCAGAAAAAAATCCAATCGTCTAACATGTTGAATCAGGCTCGATTGAAAGTATTGAAGGTTAGGGAAGATCACGTTCGTACCGTACTA
	GAGGAGGCGCGTAAACGACTTGGTCAGGTCACAAACGACCAGGGAAAATATTCCCAAATCCTGGAAAGCCTCATTTTGCAGGGATTA
	TATCAGCTTTTTGAGAAAGATGTTACCATTCGAGTTCGGCCCCAGGACCGAGAACTGGTCAAATCCATCATTCCCACCGTCACGAACA
	AGTATAAAGATGCCACCGGTAAGGACATCCATCTGAAAATTGATGACGAAATCCATCTGTCCCAAGAAACCACCGGGGGAATCGACC
	TGCTGGCGCAGAAAAACAAAATCAAGATCAGCAATACTATGGAGGCTCGTCTGGAGCTGATTTCGCAGCAACTTCTGCCCGAGATCC
	GAAGGGCGAATTCACCAGCTTTCTTGTACAAAGTGGTATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGG
	CGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGT
	TAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAAT
	TTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAA
	ATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTT
	GATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT
	TACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATC
	CGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGA
	AACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAA
	AACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAA
	CGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGC
	GATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGG
	CGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAA
	AGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAA
	CTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACA
	TAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGA
	GTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCGACCACTTTGTACAAGAAAGCTGGGTCGAATT
	CGCCCTTCGGATCTCGGGCAGAAGTTGCTGCGAAATCAGCTCCAGACGAGCCTCCATAGTATTGCTGATCTTGATTTTGTTTTTCTGC
	GCCAGCAGGTCGATTCCCCCGGTGGTTTCTTGGGACAGATGGATTTCGTCATCAATTTTCAGATGGATGTCCTTACCGGTGGCATCTT
	TATACTTGTTCGTGACGGTGGGAATGATGGATTTGACCAGTTCTCGGTCCTGGGGCCGAACTCGAATGGTAACATCTTTCTCAAAAAG
	CTGATATAATCCCTGCAAAATGAGGCTTTCCAGGATTTGGGAATATTTTCCCTGGTCGTTTGTGACCTGACCAAGTCGTTTACGCGCC
	TCCTCTAGTACGGTACGAACGTGATCTTCCCTAACCTTCAATACTTTCAATCGAGCCTGATTCAACATGTTAGACGATTGGATTTTTTT
	CTGGAGTTCGACCTGTTTCTCTTTCTTCTCATAATATTCCATAATCTTGAGACGTTGTTGCTGAACAAGGCGCCCCTTTTCAATATTAAA
	TTCTTCCTCGGCCTTGGCATCGATTTCTTCTGCCTTTTCGTTTGCCTCTTGTTCAATGAAAGCCATCATATGCTTGATCTGCGAAGGGC

LD016	SEQIDNO: 246
	GCCCTTGGAATAGGATGGGTAATGTCGTCGTTGGGCATAGTCAATATAGGAATCTGGGTGATGGATCCGTTACGTCCTTCAACACGG
	CCGGCACGTTCATAGATGGTAGCTAAATCGGTGTACATGTAACCTGGGAAACCACGACGACCAGGCACCTCTTCTCTGGCAGCAGAT
	ACCTCACGCAAAGCTTCTGCATACGAAGACATATCTGTCAAGATGACCAAGACGTGCTTCTCACATTGGTAAGCCAAGAATTCGGCAG
	CTGTCAAAGCCAGACGAGGTGTAATAATTCTTTCAATGGTAGGATCGTTGGCCAAATTCAAGAACAGGCAGACATTCTCCATAGAACC
	GTTCTCTTCGAAATCCTGTTTGAAGAACCTAGCTGTTTCCATGTTAACACCCATAGCAGCGAAAACAATAGCAAAGTTATCTTCATGAT
	CATCAAGTACAGATTTACCAGGAATCTTGACTAAACCAGCCTGTCTACAGATCTGGGCAGCAATTTCATTGTGAGGCAGACCAGCTGC
	AGAGAAAATGGGGATCTTCTGACCACGAGCAATGGAGTTCATCACGTCAATAGCTGTAATACCCGTCTGGATCATTTCCTCAGGATAG
	ATACGGGACCACGGATTGATTGGTTGACCCTGGATGTCCAAGAAGTCTTCAGCCAAAATTGGGGGACCTTTGTCGATGGGTTTTCCT
	GATCCATTGAAAACACGTCCCAACATATCTTCAGAAACAGGAGTCCTCAAAATATCTCCTGTGAATTCACAAGCGGTGTTTTTGGCGT
	CGATTCCTGATGTGCCCTCGAACACTTGAACCACAGCTTTTGACCCACTGACTTCCAGAACTTGTCCCGAACGTATAGTGCCATCAGC
	CAGTTTGAGTTGTACGATTTCATTGTACTTGGGGAACTTAACATCTTCGAGGATTACCAGAGGACCGTTCACACCAGACACAGTCAAG
	GGCGAATTCACCAGCTTTCTTGTACAAAGTGGTATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGG
	CCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAA
	CTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTG
	ACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATT
	CAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGKAAAAAATCACTGGATATACCACCGTTGAT
	ATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTAC
	GGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGG
	AATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAAC
	GTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAAC
	CTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGT
	GGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGAT
	TCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGG
	GGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGC
	CAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTA
	AAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAA
	CTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGT
	ATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGC
	CCTTGACTGTGTCTGGTGTGAACGGTCCTCTGGTAATCCTCGAAGATGTTAAGTTCCCCAAGTACAATGAAATCGTACAACTCAAACT
	GGCTGATGGCACTATACGTTCGGGACAAGTTCTGGAAGTCAGTGGGTCAAAAGCTGTGGTTCAAGTGTTCGAGGGCACATCAGGAAT
	CGACGCCAAAAACACCGCTTGTGAATTCACAGGAGATATTTTGAGGACTCCTGTTTCTGAAGATATGTTGGGACGTGTTTTCAATGGA
	TCAGGAAAACCCATCGACAAAGGTCCCCCAATTTTGGCTGAAGACTTCTTGGACATCCAGGGTCAACCAATCAATCCGTGGTCCCGT
	ATCTATCCTGAGGAAATGATCCAGACGGGTATTACAGCTATTGACGTGATGAACTCCATTGCTCGTGGTCAGAAGATCCCCATTTTCT
	CTGCAGCTGGTCTGCCTCACAATGAAATTGCTGCCCAGATCTGTAGACAGGCTGGTTTAGTCAAGATTCCTGGTAAATCTGTACTTGA
	TGATCATGAAGATAACTTTGCTATTGTTTTCGCTGCTATGGGTGTTAACATGGAAACAGCTAGGTTCTTCAAACAGGATTTCGAAGAGA
	ACGGTTCTATGGAGAATGTCTGCCTGTTCTTGAATTTGGCCAACGATCCTACCATTGAAAGAATTATTACACCTCGTCTGGCTTTGACA
	GCTGCCGAATTCTTGGCTTACCAATGTGAGAAGCACGTCTTGGTCATCTTGACAGATATGTCTTCGTATGCAGAAGCTTTGCGTGAGG
	TATCTGCTGCCAGAGAAGAGGTGCCTGGTCGTCGTGGTTTCCCAGGTTACATGTACACCGATTTAGCTACCATCTATGAACGTGCCG
	GCCGTGTTGAAGGACGTAACGGATCCATCACCCAGATTCCTATATTGACTATGCCCAACGACGACATTACCCATCCTATTCCAAGGGC

LD027	SEQIDNO 2486
	GGGAGCAGACGATCGGTTGGTTAAAATCTGGGACTATCAAAACAAAACGTGTGTCCAAACCTTGGAAGGACACGCCCAAAACGTAAC
	CGCGGTTTGTTTCCACCCTGAACTACCTGTGGCTCTCACAGGCAGCGAAGATGGTACCGTTAGAGTTTGGCATACGAATACACACAG
	ATTAGAGAATTGTTTGAATTATGGGTTCGAGAGAGTGTGGACCATTTGTTGCTTGAAGGGTTCGAATAATGTTTCTCTGGGGTATGAC
	GAGGGCAGTATATTAGTGAAAGTTGGAAGAGAAGAACCGGCAGTTAGTATGGATGCCAGTGGCGGTAAAATAATTTGGGCAAGGCAC
	TCGGAATTACAACAAGCTAATTTGAAGGCGCTGCCAGAAGGTGGAGAAATAAGAGATGGGGAGCGTTTACCTGTCTCTGTAAAAGAT
	ATGGGAGCATGTGAAATATACCCTCAAACAATCCAACATAATCCGAATGGAAGATTCGTTGTAGTATGCGGAGACGGCGAATATATCA
	TTTACACAGCGATGGCTCTACGGAACAAGGCTTTTGGAAGCGCTCAAGAGTTTGTCTGGGCTCAGGACTCCAGCGAGTATGCCATTC
	GCGAGTCTGGTTCCACAATTCGGATATTCAAAAACTTCAAAGAAAGGAAGAACTTCAAGTCGGATTTCAGCGCGGAAGGAATCTACG
	GGGGTTTTCTCTTGGGGATTAAATCGGTGTCCGGTTTAACGTTTTACGATTGGGAAACTTTGGACTTGGTGAGACGGATTGAAATACA
	ACCGAGGGCGGTTTATTGGTCTGACAGTGGAAAATTAGTCTGTCTCGCAACGGAGGACAGCTACTTCATCCTTTCTTATGATTCGGAG
	CAAGTTCAGAAAGGCCAGGGAGAACAATCAAGTCGCAGAGGATGGCGTAGAGGCCGCTTTCGATGTGTTGGGGGAAATGAACGAGTC
	TGTCCGAACCCAGCTTTCTTGTACAAAGTGGTGATATCCCGCGGGATCAGAAGCAACCTCATGGAAATGATGAGGTAAGGTTTCATAC
	TCTTGCCTCTTCTTACGGCTTTCTGTGTCTTCACTGTAAGTTTCTATGATTTGAGCCACCAATATATATGCTCTGGTGTGCTGAGTTATG
	TTTATCTGGTCACGCTTAGTGGGTAAAATTATGCTTATTTTAGCATAAACTTTAATGAGATTAGGTTTTGTATCACACCGATCTTTAGTT
	GTTTAGTAAGATGACAGAAATTCTTGGTAAAACACTCTAAATCGTCTTTCTTTAGTGAAGTTTTCCTTAGAGTAGCATAAATTTTGGCTTT
	TTTCTTGATGGTTGAATAAGGTGGCACTTGTTGGTATGAGACTTTATTGAGAGTCATATTAAGCTGATCCACGCGTTTACGCCCCGCC
	CTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC
	AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTT
	AAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTT
	CACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTC
	AGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGA
	TGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAAT
	ATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAA
	CGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGCCGGATCAGCTTAGCGTTCATTGAATTTG
	ATGGCCATAGGGGTTAGATGCAACTGTTTCTTTGAACATTGTAGAAATATATAAAGATTTTACATTAGCCTACTCTTGAAAGTCAAATT
	GTCGAATTTGATTATATTATACTCTAGAGGTGATATTAGTTAATGAGTTTATACTCGGTTATTTACAGCTTATTCATATACCAGTTAACGT
	GTCTCATATATTCTAACTTCTTAGCATTTAACGTGTTTGCAGGTCAGCTTGACACTGAACATAACAGCATCACTAGTGCGGCCGCCTG
	CAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATATACCACTTTGTACAAGAAAGCTGGTCGAATTCGCCCTTTCGG
	ACAGACTCGTTCATTTCCCCCAACACATCGAAAGCGGCCTCTACGCCATCCTCTGCGACTTGATTGTTCTCCCTGGCCTTCTGAACTT
	GCTCCGAATCATAAGAAAGGATGAAGTAGCTGTCCTCCGTTGCGAGACAGACTAATTTTCCACTGTCAGACCAATAAACCGCCCTCG
	GTTGTATTTCAATCCGTCTCACCAAGTCCAAAGTTTCCCAATCGTAAAACGTTAAACCGGACACCGATTTAATCCCCAAGAGAAAACCC
	CCGTAGATTCCTTCCGCGCTGAAATCCGACTTGAAGTTCTTCCTTTCTTTGAAGTTTTTGAATATCCGAATTGTGGAACCAGACTCGCG
	AATGGCATACTCGCTGGAGTCCTGAGCCCAGACAAACTCTTGAGCGCTTCCAAAAGCCTTGTTCCGTAGAGCCATCGCTGTGTAAAT
	GATATATTCGCCGTCTCCGCATACTACAACGAATCTTCCATTCGGATTATGTTGGATTGTTTGAGGGTATATTTCACATGCTCCCATAT
	CTTTTACAGAGACAGGTAAACGCTCCCCATCTCTTATTTCTCCACCTTCTGGCAGCGCCTTCAAATTAGCTTGTTGTAATTCCGAGTGC
	CTTGCCCAAATTATTTTACCGCCACTGGCATCCATACTAACTGCCGGTTCTTCTCTTCCAACTTTCACTAATATACTGCCCTCGTCATA
	CCCCAGAGAAACATTATTCGAACCCTTCAAGCAACAAATGGTCCACACTCTCTCGAACCCATAATTCAAACAATTCTCTAATCTGTGTG
	TATTCGTATGCCAAACTCTAACGGTACCATCTTCGCTGCCTGTGAGAGCCACAGGTAGTTCAGGGTGGAAACAAACCGCGGTTACGT
	TTTGGGCGTGTCCTTCCAAGGTTTGGACACACGTTTTGTTTTGATAGTCCCAGATTTTAACCAACCGATCGTCTGCTCCC

TABLE 9-PC

	Hairpin Sequence
Target ID
	5′ → 3′

PC001	SEQ ID NO: 508
	AGATTCAAATTTGATGTAGTCAAGAATTTTAGATGTAGCAATTTCCATTTGAATTGTGTCATTCACTTTGATGTTGGGGTCAGGGTAACGA
	ATGGTTCTGCCATCATGTGTTACCAAAAATGGGATTCCTTTGGGACCAGTTTGGACTCTCCTTACTTTACACAACTTGTATTTTGCCTCTT
	CAGCTGTAATACGGTGCACAGCAAATCTTCCTTTAACATCATAGATCAGACGGAAAAATTCACCAGTCTTCTCAATAGTAATGACATCCA
	TGAAACCAGCAGGGTAATTAGAATCAGTCCTCACTTTACCATCAACTTTGATCAACCTTTGCATGACAATTTTAGTGACTTCACTGTTTGT
	AAGGGCATACTTCAGCCTGTTACGAAGGAAAATCACTAAAGGCAGGGATTCGCGCAACTTGTGAGGCCCGGTGGATGGACGAGGGGC
	GAAGACACCCCCCAATTTGTCCAACATCCATGCAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCC
	GCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTT
	AAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCAC
	CTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAA
	CAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGG
	AGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTA
	CCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACA
	TTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGT
	TACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCG
	CAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGT
	GAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGA
	CAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGT
	ACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTT
	ATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTC
	TGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAG
	CGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCG
	TAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAA
	GCTGGGTCGAATTCGCCCTTGCATGGATGTTGGACAAATTGGGGGGTGTCTTCGCCCCTCGTCCATCCACCGGGCCTCACAAGTTGCG
	CGAATCCCTGCCTTTAGTGATTTTCCTTCGTAACAGGCTGAAGTATGCCCTTACAAACAGTGAAGTCACTAAAATTGTCATGCAAAGGTT
	GATCAAAGTTGATGGTAAAGTGAGGACTGATTCTAATTACCCTGCTGGTTTCATGGATGTCATTACTATTGAGAAGACTGGTGAATTTTT
	CCGTCTGATCTATGATGTTAAAGGAAGATTTGCTGTGCACCGTATTACAGCTGAAGAGGCAAAATACAAGTTGTGTAAAGTAAGGAGAG
	TCCAAACTGGTCCCAAAGGAATCCCATTTTTGGTAACACATGATGGCAGAACCATTCGTTACCCTGACCCCAACATCAAAGTGAATGAC
	ACAATTCAAATGGAAATTGCTACATCTAAAATTCTTGACTACATCAAATTTGAATCT

PC010	SEQ ID NO: 509
	CTCTCAAGGATTCTTTGCAGATGTCGCTCAGCCTATTACCGCCCAACGCGTTGATTGGATTGATCACGTTCGGAAAAATGGTGCAAGTC
	CACGAACTGGGTACCGAAGGCTGCAGCAAGTCGTACGTGTTCTGTGGAACGAAAGATCTCACCGCCAAGCAAGTCCAGGAGATGTTG
	GGCATTGGAAAAGGGTCACCAAATCCCCAACAACAGCCAGGGCAACCTGGGCGGCCAGGGCAGAATCCCCAAGCTGCCCCTGTACCA
	CCGGGGAGCAGATTCTTGCAGCCCGTGTCAAAATGCGACATGAACTTGACAGATCTGATCGGGGAGTTGCAGAAAGACCCTTGGCCC
	GTACATCAGGGCAAAAGACCTCTTAGATCCACAGGCGCAGCATTGTCCATCGCTGTCGGCCTCTTAGAATGCACCTATCCGAATACGG
	GTGGCAGAATCATGATATTCTTAGGAGGACCATGCTCTCAGGGTCCCGGCCAGGTGTTGAACGACGATTTGAAGCAGCCCATCAGGTC
	CCATCATGACATACACAAAGACAATGCCAAGTACATGAAGAAGGCTATCAAACATTACGATCACTTGGCAATGCGAGCTGCCACCAACA
	GCCATTGCATCGACATTTACTCCTGCGCCCTGGATCAGACGGGACTGATGGAGATGAAGCAGTGCTGCAATTCCACCGGAGGGCACAT
	GGTCATGGGCGATTCCTTCAATTCCTCTCTATTCAAACAAACCTTCCAGCGAGTGTTCTCAAAAGACCCGAAGAACGACCTCAAGATGG
	CGTTCAACGCCACCTTGGAGGTGAAGTGTTCCAGGGAGTTAAAAGTCCAAGGGGGCATCGGCTCGTGCGTGTCCTTGAACGTTAAAAG
	CCCTCTGGTTTCCGATACGGAACTAGGCATGGGGAATACTGTGCAGTGGAAACTTTGCACGTTGGCGCCGAGCTCTACTGTGGCGCTG
	TTCTTCGAGGTGGTTAACCAGCATTCGGCGCCCATACCACAGGGAGGCAGGGGCTGCATCCAGCTCATCACCCAGTATCAGCACGCG
	AGCGGGCAAAGGAGGATCAGAGTGACCACGATTGCTAGAAATTGGGCGGACGCTACTGCCAACATCCACCACATTAGCGCTGGCTTC
	GACCAAGAAGCGGCGGCAGTTGTGATGGCCCGAATGGCCGGTTACAAGGCGGAATCGGACGAGACTCCCGACGTGCTCAGATGGGT
	GGACAGGATGTTGATCAGGCTGTGCCAGAAGTTCGGAGAGTACAATAAAGACGATCCGAATTCGTTCAGGTTGGGGGAGAACTTCAGT
	CTGTATCCGCAGTTCATGTACCATTTGAGACGGTCGCAGTTTCTGCAGGTGTTCAATAATTCTCCTGATGAAACGTCGTTTTATAGGCAC
	ATGCTGATGCGTGAGGATTTGACTCAGTCTTTGATCATGATCCAGCCGATTTTGTACAGTTACAGCTTCAACGGGCCGCCCGAGCCTGT
	GTTGTTGGACACAAGCTCTATTCAGCCGGATAGAATCCTGCTCATGGACACTTTCTTCCAGATACTCATTTTCCATGGAGAGACCATTGC
	CCAATGGCGAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTC
	GACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATAT
	GAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAAT
	TCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGG
	CCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACC
	ACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTG
	GATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCT
	CATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAAC
	TGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTG
	AAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAA
	CGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCG
	ATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGG
	GGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCA
	AAATTTATGCTACTCTAAGGAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAG
	ATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCA
	GCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAA
	CCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTTCGC
	CATTGGGCAATGGTCTCTCCATGGAAAATGAGTATCTGGAAGAAAGTGTCCATGAGCAGGATTCTATCCGGCTGAATAGAGCTTGTGTC
	CAACAACACAGGCTCGGGCGGCCCGTTGAAGCTGTAACTGTACAAAATCGGCTGGATCATGATCAAAGACTGAGTCAAATCCTCACGC
	ATCAGCATGTGCCTATAAAACGACGTTTCATCAGGAGAATTATTGAACACCTGCAGAAACTGCGACCGTCTCAAATGGTACATGAACTG
	CGGATACAGACTGAAGTTCTCCCCCAACCTGAACGAATTCGGATCGTCTTTATTGTACTCTCCGAACTTCTGGCACAGCCTGATCAACA
	TCCTGTCCACCCATCTGAGCACGTCGGGAGTCTCGTCCGATTCCGCCTTGTAACCGGCCATTCGGGCCATCACAACTGCCGCCGCTTC
	TTGGTCGAAGCCAGCGCTAATGTGGTGGATGTTGGCAGTAGCGTCCGCCCAATTTCTAGCAATCGTGGTCACTCTGATCCTCCTTTGCC
	CGCTCGCGTGCTGATACTGGGTGATGAGCTGGATGCAGCCCCTGCCTCCCTGTGGTATGGGCGCCGAATGCTGGTTAACCACCTCGA
	AGAACAGCGCCACAGTAGAGCTCGGCGCCAACGTGCAAAGTTTCCACTGCACAGTATTCCCCATGCCTAGTCCGTATCGGAAACCAG
	AGGGCTTTTAACGTTCAAGGACACGCACGAGCCGATGCCCCCTTGGACTTTTAACTCCCTGGAACACTCACCTCCAAGGTGGCGTTG
	AACGCCATCTTGAGGTCGTTCTTCGGGTCTTTTGAGAACACTCGCTGGAAGGTTTGTTTGAATAGAGAGGAATGAAGGTCGCCCAT
	GACCATGTGCCCTCCGGTGGAATTGCAGCACTGCTTCATCTCCATCAGTCCCGTCTGATCCAGGGCGCAGGAGTAAATGTCGATGCAA
	TGGCTGTTGGTGGCAGCTCGCATTGCCAAGTGATCGTAATGTTTGATAGCCTTCTTCATGTACTTGGCATTGTCTTTGTGTATGTCATGA
	TGGGACCTGATGGGCTGCTTCAAATCGTCGTTCAACACCTGGCCGGGACCCTGAGAGCATGGTCCTCCTAAGAATATCATGATTCTGC
	CACCCGTATTCGGATAGGTGCATTCTAAGAGGCCGACAGCGATGGACAATGCTGCGCCTGTGGATCTAAGAGGTCTTTTGCCCTGATG
	TACGGGCCAAGGGTCTTTCTGCAACTCCCCGATCAGATCTGTCAAGTTCATGTCGCATTTTGACACGGGCTGCAAGAATCTGCTCCCCG
	GTGGTACAGGGGCAGCTTGGGGATTCTGCCCTGGCCGCCCAGGTTGCCCTGGCTGTTGTTGGGGATTTGGTGACCCTTTTCCAATGC
	CCAACATCTCCTGGACTTGCTTGGCGGTGAGATCTTTCGTCCACAGAACACGTACGACTGCTGCAGCCTTCGGTACCCAGTTCGTG
	GACTTGCACCATTTTTCCGAACGTGATCAATCCAATCAACGCGTTGGGCGGTAATAGGCTGAGCGACATCTGCAAAGAATCCTGAGAG

PC014	SEQ ID NO: 510
	CGCAGATCAAACATATGATGGCTTTCATTGAACAAGAAGCCAATGAGAAAGCAGAAGAATCGATGCCAAGGCAGAGGAGGAATTCAAC
	ATTGAAAAAGGGCGTTTAGTCCAGCAACAGAGACTCAAGATCATGGAGTACTACGAGAAAAAGGAGAAGCAAGTCGAACTTCAAAAGAA
	AATTCAGTCCTCTAATATGTTGAATCAGGCTCGTTTGAAGGTGCTGAAAGTGAGAGAGGACCATGTCAGAGCAGTCCTGGAGGATGCTC
	GTAAAAGTCTTGGTGAAGTAACCAAAGACCAAGGAAAATACTCCCAAATTTTGGAGAGCCTAATCCTACAAGGACTGTTCCAGCTGTTC
	GAGAAGGAGGTGACGGTCCGCGTGAGACCGCAAGATAGGGACTTGGTTAGGTCCATCCTGCCCAACGTCGCTGCCAAATACAAGGAC
	GCCACCGGCAAAGACATCCTACTCAAGGTGGACGATGAGTCGCACCTGTCTCAGGAGATCACCGGAGGCGTCGATCTGCTCGCTCAG
	AAGAACAAGATCAAGATCAGCAACACGATGGAGGCTAGGTGGATCTGATCGCTCAGCAATTGGTGCCCGAGATCCGAAGGGCGAATT
	CGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACT
	AGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATAT
	GAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAG
	TAGGCTAATGTAAAATCTTTATATTTCTACAATGTTCAAAGAAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTA
	AGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGC
	ATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATACGGCCTTTTTAAAGA
	CCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAA
	TGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGG
	AGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAA
	GGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTC
	TTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCT
	GTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGC
	TTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAA
	AACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAA
	TCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATG
	GTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGA
	GGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTCGGATCTCGGGCACCAATTGCTGA
	GCGATCAGATCCAACCTAGCCTCCATCGTGTTGCTGATCTTGATCTTGTTCTTCTGAGCGAGCAGATCGACGCCTCCGGTGATCTCCTG
	AGACAGGTGCGACTCATCGTCCACCTGAGTAGGATGTCTTTGCCGGTGGCGTCCTTGTATTTGGCAGCGACGTTGGGCAGGATGGAC
	CTAACCAAGTCCCTATCTTGCGGTCTCACGCGGACCGTCACCTCCTTCTCGAACAGCTGGAACAGTCCTTGTAGGATTAGGCTCTCCAA
	AATTTGGGAGTATTTTCCTTGGTCTTTGGTTACTTCACCAAGACTTTTACGAGCATCCTCCAGGACTGCTCTGACATGGTCCTCTCTCAC
	TTTCAGCACCTTCAAACGAGCCTGATTCAACATATTAGAGGACTGAATTTTCTTTTGAAGTTCGACTTGCTTCTCCTTTTTCTCGTAGTAC
	TCCATGATCTTGAGTCTCTGTTGCTGGACTAAACGCCCTTTTTCAATGTTGAATTCCTCCTCTGCCTTGGCATCGATTTCTTCTGCTTTCT
	CATTGGCTTCTTGTTCAATGAAAGCCATCATATGTTTGATCTGCG

PC016	SEQ ID NO: 511
	TTGGGCATAGTCAAGATGGGGATCTGCGTGATGGAGCCGTTGCGGCCCTCCACACGACCGGCGCGCTCGTAAATGGTGGCCAGATCG
	GTGTACATGTAACCGGGGAAACCCCTACGGCCGGGCACTTCTTCTCGAGCGGCAGACACCTCACGCAACGCCTCCGCGTACGACGAC
	ATGTCGGTCAAGATGACCAGCACGTGCTTCTCGCACTGGTAGGCCAAGAATTCGGCGGCCGTCAGAGCCAAACGCGGCGTGATGATG
	CGCTCGATGGTCGGATCGTTGGCCAAGTTCAAGAACAGACACACGTTCTCCATCGAGCCGTTCTCTTCGAAGTCCTGCTTGAAGAACCT
	GGCAGTTTCCATGTTGACACCCATAGCAGCAAACACAATAGCAAAGTTGTCTTCATGGTCATCCAGCACAGACTTGCCAGGTACTTTGA
	CCAAGCCAGCCTGCCTACAAATCTGGGCTGCAATCTCATTGTGGGGCAGCCCAGCGGCGGAGAAGATCGGAATCTTCTGCCCTCTGG
	CGATAGAGTTCATCACGTCGATGGCCGTGATCCCAGTCTGGATCATTTCCTCGGGATAAATACGCGACCACGGGTTGATCGGCTGTCC
	TTGGATGTCGAGGTAGTCCTCAGCCAGGATCGGGGGACCTTTATCAATGGGTTTTCCTGATCCATTGAAGACACGTCCCAGCATATCTT
	CTGATACTGGAGTTCTTAGAATATCTCCAGTGAACTCACACACCGTGTTCTTAGCATCAATACCTGATGTGCCTCAAATACCTGAACAA
	CTGCCTTTGATCCACTGACTTCCAAAACTTGTCCAGATCGTAGAGTTCCATCTGCCAATTTGAGCTGGACAATTTCATTGAATTTTGGAA
	ACTTGACATCCTCAAGAATGACCAGTAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCA
	GGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCT
	AAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGA
	GTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGC
	ATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAA
	AATCACTGGATATACCACCGTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAA
	CCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGC
	CCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACC
	GTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGAT
	GTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTC
	ACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGT
	GCTGATGCCGCTGGCGATCAGGTCATCATGCCGTCTGTGATGGCTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCG
	ATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAAC
	CATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATC
	TACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGAC
	CAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAA
	GAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGG
	TCGAATTCGCCCTTACTGGTCATTCTTGAGGATGTCAAGTTTCCAAAATTCAATGAAATTGTCCAGCTCAAATTGGCAGATGGAACTCTA
	CGATCTGGACAAGTTTTGGAAGTCAGTGGATCAAAGGCAGTTGTTCAGGTATTTGAAGGCACATCAGGTATTGATGCTAAGAACACGGT
	GTGTGAGTTCACTGGAGATATTCTAAGAACTCCAGTATCAGAAGATATGCTGGGACGTGTCTTCAATGGATCAGGAAAACCCATTGATA
	AAGGTCCCCCGATCCTGGCTGAGGACTACCTCGACATCCAAGGACAGCCGATCAACCCGTGGTCGCGTATTTATCCCGAGGAAATGAT
	CCAGACTGGGATCACGGCCATCGACGTGATGAACTCTATCGCCAGAGGGCAGAAGATTCCGATCTTCTCCGCCGCTGGGCTGCCCCA
	CAATGAGATTGCAGCCCAGATTTGTAGGCAGGCTGGCTTGGTCAAAGTACCTGGCAAGTCTGTGCTGGATGACCATGAAGACAACTTT
	GCTATTGTGTTTGCTGCTATGGGTGTCAACATGGAAACTGCCAGGTTCTTCAAGCAGGACTTCGAAGAGAACGGCTCGATGGAGAACG
	TGTGTCTGTTCTTGAACTTGGCCAACGATCCGACCATCGAGCGCATCATCACGCCGCGTTTGGCTCTGACGGCCGCCGAATTCTTGGC
	CTACCAGTGCGAGAAGCACGTGCTGGTCATCTTGACCGACATGTCGTCGTACGCGGAGGCGTTGCGTGAGGTGTCTGCCGCTCGAGA
	AGAAGTGCCCGGCCGTAGGGGTTTCCCCGGTTACATGTACACCGATCTGGCCACCATTTACGAGCGCGCCGGTCGTGTGGAGGGCCG
	CAACGGCTCCATCACGCAGATCCCCATCTTGACTATGCCCAA

PC027	SEQ ID NO: 512
	GGGCCAAGCACAGCGAAATGCAGCAAGCTAACTTGAAAGCACTACCAGAAGGAGCTGAAATCAGAGATGGAGAACGTTTGCCAGTCAC
	AGTAAAGGACATGGGAGCATGCGAGATTTACCCACAAACAATCCAACACAACCCCAATGGGCGGTTTGTAGTGGTTTGTGGTGATGGA
	GAATACATAATATACACGGCTATGGCCCTTCGTAACAAAGCATTTGGTAGCGCTCAAGAATTTGTATGGGCACAGGACTCCAGTGAATA
	TGCCATCCGCGAATCCGGATCCACCATTCGAATCTTCAAGAATTTCAAAGAAAAAAAGAATTTCAAGTCCGACTTTGGTGCCGAAGGAAT
	CTATGGTGGTTTTCTCTTGGGTGTGAAATCAGTGTCTGGCTTAGCTTTCTATGACTGGGAAACGCTTGAGTTAGTAAGGCGCATTGAAAT
	ACAGCCTAGAGCTATCTACTGGTCAGATAGTGGCAAGTTGGTATGCCTTGCTACCGAAGATAGCTATTTCATATTGTCCTATGACTCTGA
	CCAAGTCCAGAAAGCTAGAGATAACAACCAAGTGCCGAAGATGGAGTGGAGGCTGCCTTTGATGTCCTAGGTGAAATAAATGAATCC
	GTAAGAACAGGTCTTTGGGTAGGAGACTGCTTCATTTACACAAACGCAGTCAACCGTATCAACTACTTTGTGGGTGGTGAATTGGTAAC
	TATTGCACATCTGGACCGTCCTCTATATGTCCTGGGCTATGTACCTAGAGATGACAGGTTATACTTGGTTGATAAAGAGTTAGGAGTAGT
	CAGCTATCAATTGCTATTATCTGTACTCGAATATCAGACTGCAGTCATGCGACGAGACTTCCCAACGGCTGATCGAGTATTGCCTTCAAT
	TCCAAAAGAACACCGCACTAGGGTGGCACAAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGC
	CTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAA
	ATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCT
	CTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACA
	GTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAG
	AAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACC
	TATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTC
	TTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTAC
	ACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAA
	GATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAG
	TTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAA
	GGTGCTGATGCCGCTGGCGATTCAGGTCATCATGCCGTCTGTGATGGCTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACT
	GCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATT
	CAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGT
	CATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCG
	TGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAA
	GAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCT
	GGGTCGAATTCGCCCTTTGTGCCACCCTAGTGCGGTGTTCTTTTGGAATTGAAGGCAATACTCGATCAGCCGTTGGGAAGTCTCGTCG
	CATGACTGCAGTCTGATATTCGAGTACAGATAATAGCAATTGATAGCTGACTACTCCTAACTCTTTATCAACCAAGTATAACCTGTCATCT
	CTAGGTACATAGCCCAGGACATATAGAGGACGGTCCAGATGTGCAATAGTTACCAATTCACCACCCACAAAGTAGTTGATACGGTTGAC
	TGCGTTTGTGTAAATGAAGCAGTCTCCTACCCAAAGACCTGTTCTTACGGATTCATTTATTTCACCTAGGACATCAAAGGCAGCCTCCAC
	TCCATCTTCGGCAACTTGGTTGTTATCTCTAGCTTTCTGGACTTGGTCAGAGTCATAGGACAATATGAAATAGCTATCTTCGGTAGCAAG
	GCATACCAACTTGCCACTATCTGACCAGTAGATAGCTCTAGGCTGTATTTCAATGCGCCTTACTAACTCAAGCGTTTCCCAGTCATAGAA
	AGCTAAGCCAGACACTGATTTCACACCCAAGAGAAAACCACCATAGATTCCTTCGGCACCAAAGTCGGACTTGAAATTCTTTTTTTCTTT
	GAAATTCTTGAAGATTCGAATGGTGGATCCGGATTCGCGGATGGCATATTCACTGGAGTCCTGTGCCCATACAAATTCTTGAGCGCTAC
	CAAATGCTTTGTTACGAAGGGCCATAGCCGTGTATATTATGTATTCTCCATCACCACAAACCACTACAAACCGCCCATTGGGGTTGTGTT
	GGATTGTTTGTGGGTAAATCTCGCATGCTCCCATGTCCTTTACTGTGACTGGCAAACGTTCTCCATCTCTGATTCAGCTCCTTCTGGTA
	GTGCTTTCAAGTTAGCTTGCTGCATTTCGCTGTGCTTGGCCC

TABLE 9-MP

	Hairpin Sequence
Target ID
	5′ → 3′

MP001	SEQ ID NO: 1066
	GTTTAAACGCACCCAAAGCATGGATGTTGGACAAATCGGGGGGTGTCTTCGCTCCACGTCCAAGCACCGGTCCACACAAACTTCGTG
	AATCACTACCGTTATTGATCTTGCGTAATCGTTTGAAGTATGCACTTACTGGTGCCGAAGTCACCAAGATTGTCATGCAAAGATTA
	ATCAAGGTTGATGGCAAAGTCCGTACCGACCCTAATTATCCAGCCGGTTTTATGGATGTTATATCTATCCAAAAGACCAGTGAGCACT
	TTAGATTGATCTATGATGTGAAAGGTCGTTTCACCATCCACAGAATTACTCCTGAAGAAGCAAAATACAAGTGTGTAAAGTAAAGAGG
	GTACAAACTGGACCCAAAGGTGTGCCATTTTTAACTACTCATGATGGCCGTACTATTCGCTACCCTGACCCTAACATCAAGGTTAATG
	ACACTATTAGATACGATATTGCATCATCTAAAATTTTGGATCATATCCGTTTTGAAACTGGAAACTTGTGCATGATAACTGGAGGTCGC
	AATTTAGGGCGTGTTGGTATTGAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGG
	TCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTA
	AGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGA
	GTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTCTACAATGTTCAAAGAAACAGTTG
	CATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAA
	AAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTA
	TAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTC
	TTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTT
	ACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCG
	CAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGG
	TGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGC
	GACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAA
	CAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCC
	ACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAA
	GAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTAC
	CCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACAC
	AGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTT
	GTACAAGAAAGCTGGGTCGAATTCGCCCTTCAATACCAACACGCCCTAAATTGCGACCTCCAGTTATCATGCACAAGTTTCCAGTTTC
	AAAACGGATATGATCCAAAATTTTAGATGATGCAATATCGTATCTAATAGTGTCATTAACCTTGATGTTAGGGTCAGGGTAGCGAATAG
	TACGGCCATCATGAGTAGTTAAAAATGGCACACCTTTGGGTCCAGTTTGTACCCTCTTTACTTTACACAACTTGTATTTTGCTTCTTCA
	GGAGTAATTCTGTGGATGGTGAAACGACCTTTCACATCATAGATCAATCTAAAGTGCTCACTGGTCTTTTGGATAGATATAACATCCAT
	AAAACCGGCTGGATAATTAGGGTCGGTACGGACTTTGCCATCAACCTTGATTAATCTTTGCATGACAATCTTGGTGACTTCGGCACCA
	GTAAGTGCATACTTCAAACGATTACGCAAGAAGATCAATAACGGTAGTGATTCACGAAGTTTGTGTGGACCGGTGCTTGGACGTGGA
	GCGAAGACACCCCCCGATTTGTCCAACATCCATGCTTTGGGTGCGTTTAAAC

MP002	SEQ ID NO: 1067
	GCTGATTTAAGTGCATCTGCTGCAGTTTTCATGGTAGTCAATACTGCTGTATTTGTGTTGGCACCTTCTAATGCCTCCCGCTGTTGTTC
	AATAGTTACATGGTACCATCAATTTGGGCTAATTGTTGTTCGTACCGTTTCTTACGCTTCAATGCTTGCAATGCAGCTCGTTTATTAGT
	TGTACCATTTTTTTTGGCTATCGCTACTTCTTGTTCAATTTTTTTTTCTAAAAATTCTTGTTTCTTTATCAGCATCTCTTCAGTGGATCGAA
	GCTTTTGTATCGCATCTTCGGTTGATGGTCCCTTCTCTTCCTTTTTGCCACCAAGGGCGAATTCGACCCAGCTTTCTTGTACAAAGTG
	GTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTTATGTTCAGTGT
	CAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGTAAATAACCGAG
	TATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTA
	TATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATCCGGCGAGATTT
	TCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTG
	AGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAG
	CACAAGTTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGC
	TGGTGATATGGGATAGTGTTCACCCTTGTTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGA
	CGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAG
	AATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGT
	TTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTT
	CCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCAGCTTAATATGA
	CTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTTATGCTACTCTAAGGAAAACTTCA
	CTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCTCAT
	TAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATATATATTGGTGG
	CTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGT
	TGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTTGGTGGCAAAAAGGAAGAGAAGGGACC
	ATCAACCGAAGATGCGATACAAAAGCTTCGATCCACTGAAGAGATGCTGATAAAGAAACAAGAATTTTTAGAAAAAAAAATTGAACAAG
	AAGTAGCGATAGCCAAAAAAAATGGTACAACTAATAAACGAGCTGCATTGCAAGCATTGAAGCGTAAGAAACGGTACGAACAACAATT
	AGCCCAAATTGATGGTACCATGTTAACTATTGAACAACAGCGGGAGGCATTAGAAGGTGCCAACACAAATACAGCAGTATTGACTACC
	ATGAAAACTGCAGCAGATGCACTTAAATCAGC

MP010	SEQ ID NO: 1068
	CAGACCCTGTTCAGAATATGATGCATGTTAGTGCTGCATTTGATCAAGAAGCATCTGCCGTTTTAATGGCTCGTATGGTAGTGAACCG
	TGCTGAAACTGAGGATAGTCCAGATGTGATGCGTTGGGCTGATCGTACGCTTATACGCTTGTGTCAAAAATTTGGTGATTATCAAAAA
	GATGATCCAAATAGTTTCCGATTGCCAGAAAACTTCAGTTTATATCCACAGTTCATGTATCATTTAAGAAGGTCTCAATTTCTACAAGTT
	TTTAATAATAGTCCTGATGAAACATCATATTATAGGCACATGTTGATGCGTGAAGATGTTACCCAAAGTTTAATCATGATACAGCCAATT
	CTGTATAGCTATAGTTTTAATGGTAGGCCAGAACCTGTACTTTTGGATACCAGTAGTATTCAACCTGATAAAATATTATTGATGGACAC
	ATTTTTCCATATTTTGATATTCCATGGAGAGACTATTGCTCAATGGAGAGCAATGGATTATCAAAATAGACCAGAGTATAGTAACCTCA
	AGCAGTTGCTTCAAGCCCCCGTTGATGATGCTCAGGAAATTCTCAAAACTCGATTCCCAATGCAAGGGCGAATTCGACCCAGCTTTCT
	TGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATGCTGTT
	ATGTTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGT
	AAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTCAAGAGTAGGCTAAT
	GTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTGATC
	CGGCGAGATTTTCAGGAGCTAAGGAAGCTTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAA
	AGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAA
	AGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAA
	GACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATTAGCAAACTGAAACGTTTTCATCGCTCTGGAGTG
	AATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTGACGGTGAAAACCTGGCCTATTTCCCTAAAGG
	GTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCT
	TCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCT
	GTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCA
	GCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCCAAAATTTATGCTACTCTAAG
	GAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAA
	CCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCAGCACACCAGAGCATA
	TATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCAT
	TTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTTGCATTGGGAATCGAG
	TTTTGAGAATTTCCTGAGCATCATCAACGGGGGCTTGAAGCAACTGCTTGAGGTTACTATACTCTGGTCTATTTTGATAATCCATTGCT
	CTCCATTGAGCAATAGTCTCTCCATGGAATATCAAAATATGGAAAAATGTGTCCATCAATAATATTTTATCAGGTTGAATACTACTGGTA
	TCCAAAAGTACAGGTTCTGGCCTACCATTAAAACTATAGCTATACAGAATTGGCTGTATCATGATTAAACTTTGGGTAACATCTTCACG
	CATCAACATGTGCCTATAATATGATGTTTTCATCAGGACTATTATTAAAAACTTGTAGAAATTGAGACCTTCTTAAATGATACATGAACTG
	TGGATATAAACTGAAGTTTTCTGGCAATCGGAAACTATTTGGATCATCTTTTTGATAATCACCAAATTTTTGACACAAGCGTATAAGCGT
	ACGATCAGCCCAACGCATCACATCTGGACTATCCTCAGTTTTCAGCACGGTTCACTACCATACGAGCCATTAAAACGGCAGATGCTTCT
	TGATCAAATGCAGCACTAACATGCATCATATTCTGAACAGGGTCTG

MP016	SEQ ID NO: 1069
	GTTTTCAATGGCAGTGGAAAGCCGATAGATAAAGGACCTCCTATTTTGGCTGAAGATTATTTGGATATTGAAGGCCAACCTATTAATCC
	ATACTCCAGAACATATCCTCAAGAAATGATTCAAACTGGTATTTCAGCTATTGATATCATGAACTCTATTGCTCGTGGACAAAAAATTCC
	AATATTTTCAGCTGCAGGTTTACCACATAATGAGATTGCTGCTCAAATTTGTAGACAAGCTGGTCTCGTTAAAAAACCTGGTAAATCAG
	TTCTTGACGATCATGAAGACAATTTTGCTATAGTATTTGCTGCTATGGGTGTTAATATGGAAACAGCCAGATTCTTTAAACAAGATTTTG
	AGGAAAATGGTTCAATGGAGAATGTTTGTTTGTTCTTGAATTTAGCTAATGATCCTACTATTGAGCGTATCATTACACCACGAAGGGCG
	AATTCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCC
	GCACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACT
	GGTATATGAATAAGCTGTAAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGAC
	TTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCA
	ATGAACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATAT
	ATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTTACG
	GCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGA
	ATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACG
	TTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACC
	TGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTG
	GCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATT
	CAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGG
	GCGTAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAAGCC
	AAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAA
	AGATCGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAAC
	TCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTAT
	GAAACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCC
	TTCGTGGTGTAATGATACGCTCAATAGTAGGATCATTAGCTAAATTCAAGAACAAACAAACATTCTCCATTGAACCATTTTCCTCAAAAT
	CTTGTTTAAAGAATCTGGCTGTTTCCATATTAACACCCATAGCAGCAAATACTATAGCAAAATTGTCTTCATGATCGTCAAGAACTGATT
	TACCAGGTTTTTTAACGAGACCAGCTTGTCTACAAATTTGAGCAGCAATCTCATTATGTGGTAAACCTGCAGCTGAAAATATTGGAATT
	TTTTGTCCACGAGCAATAGAGTTCATGATATCAATAGCTGAAATACCAGTTTGAATCATTTCTTGAGGATATGTTCTGGAGTATGGATT
	AATAGGTTGGCCTTCAATATCCAAATAATCTTCAGCCAAAATAGGAGGTCCTTTATCTATCGGCTTTTCCACTGCCATTGAAAAC

MP027	SEQ ID NO: 1070
	CCAAAAATACCATCTGCTCCACCTTCTGGTTTAAAAGACTTTTTTTCTTTAAAATTTTTAAAAACTTTGATTGTAGAAGAATTTTCTCTAA
	TGGCATACTCAGAATCAGAAGACCATACAAAATCCTGAGCGGAGCCAAATGCTTTATTACGCAAAGCCATTGATGTATATATAATATAC
	TCTCCATCACCACATACTACTAAAAATCTACCATTCGGATTATGAGATATTGACTGTGGATAAATTTCACAGCTACCCATGTCTTTAACT
	TGTATTGGTAAACGTTCACCATCTTTGATTTCGGCTCCTTCTGCTTGAAGCATCGCTTTAAGGTTAGCTTGTTGAATTTCACTATGACG
	TGCCCAAACAATTTTACCCCCATGAACATCCATTGACATTGCTGGCTCTTCACGACCAACTTTAACCATTATACTTCCTTCATCATAACC
	TAGAGCTACATTATTAGATCCCCGTAAGCAACAGATTGTCCATACACGTTCTAACCCATAGTTTAATGATGATTCTAATCGATAAGTAC
	CAGAATGCCAAATTCTGACGGTACCATCTTCTGAGCCAGTTAACACGATGGGAAGTTCTGGATGGAAACAAACGAGCAAGGGCGAAT
	TCGACCCAGCTTTCTTGTACAAAGTGGTGATATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGTCGACCTGCAGGCGGCCGC
	ACTAGTGATGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACACGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGT
	ATATGAATAAGCTGTAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATTCGACAATTTGACTTTC
	AAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTCAAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGA
	ACGCTAAGCTGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCC
	CAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCT
	TTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTC
	CGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTT
	CATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGG
	CCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTTCACCAGTTTTGATTTAAACGTGGCC
	AATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAG
	GTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCG
	TAAACGCGTGGATCAGCTTAATATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTTATTCAACCATCAAGAAAAAAGCCAAAA
	TTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTACCAAGAATTTCTGTCATCTTACTAAACAACTAAGAT
	CGGTGTGATACAAAACCTAATCTCATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACATAACTCA
	GCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGACACAGAAAGCCGTAAGAAGAGGCAAGAGTATGA
	AACCTTACCTCATCATTTCCATGAGGTTGCTTCTGATCCCGCGGGATATCACCACTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTT
	GCTCGTTTGTTTCCATCCAGAACTTCCCATCGTGTTAACTGGCTCAGAAGATGGTACCGTCAGAATTTGGCATTCTGGTACTTATCGAT
	TAGAATCATCATTAAACTATGGGTTAGAACGTGTATGGACAATCTGTTGCTTACGGGGATCTAATAATGTAGCTCTAGGTTATGATGAA
	GGAAGTATAATGGTTAAAGTTGGTCGTGAAGAGCCAGCAATGTCAATGGATGTTCATGGGGGTAAAATTGTTTGGGCACGTCATAGT
	GAAATTCAACAAGCTAACCTTAAAGCGATGCTTCAAGCAGAAGGAGCCGAAATCAAAGATGGTGAACGTTTACCAATACAAGTTAAAG
	ACATGGGTAGCTGTGAAATTTATCCACAGTCAATATCTCATAATCCGAATGGTAGATTTTTAGTAGTATGTGGTGATGGAGAGTATATT
	ATATATACATCAATGGCTTTGCGTAATAAAGCATTTGGCTCCGCTCAGGATTTTGTATGGTCTTCTGATTCTGAGTATGCCATTAGAGA
	AAATTCTTCTACAATCAAAGTTTTTAAAAATTTTAAAGAAAAAAAGTCTTTTAAACCAGAAGGTGGAGCAGATGGTATTTTTGG

	TABLES 10-NL (a)

	Mean % survival (days post start)	Survival

RNAi

	0	1	2	3	4	5	6	7	8	analysis¹

gfp	100	98	90	82	68	60	44	32	20	−
diet only	100	98	96	86	74	68	58	54	38	−
NL002	100	98	90	76	68	34	6	0	0	+
NL003	100	98	74	48	36	22	12	2	0	+
NL005	100	100	74	56	40	20	16	6	4	+
NL010	100	96	74	56	48	30	18	12	8	+

	Chi squared	P value	Sig. Dif.²

diet versus:
NL002	29.06	<0.0001	Yes
NL003	39.59	<0.0001	Yes
NL005	29.55	<0.0001	Yes
NL010	21.04	<0.0001	Yes
gfp dsRNA versus:
NL002	15.09	0.0001	Yes
NL003	22.87	<0.0001	Yes
NL005	15.12	<0.0001	Yes
NL010	8.838	0.0029	Yes
diet versus gfp dsRNA	4.030	0.0447 (~0.05)	No

¹= Data were analysed using Kaplan-Meier survival curve analysis
²alpha <0.05

	TABLES 10-NL (b)

	Mean % survival (days post start)	Survival

RNAi

	0	1	2	3	4	5	6	7	8	analysis¹

gfp	100	96	84	82	76	70	54	50	44	−
diet only	100	96	88	82	76	70	54	50	44	−
NL009	100	94	75	63	42	30	24	22	14	+
NL016	100	94	84	78	54	44	36	18	14	+

	Chi squared	P value	Sig. Dif.²

diet versus:
NL009	11.98	0.0005	Yes
NL016	8.98	0.0027	Yes
gfp dsRNA versus:
NL009	13.69	0.0002	Yes
NL016	11.37	0.0007	Yes
diet versus gfp dsRNA	0.03317	0.8555	No

¹= Data were analysed using Kaplan-Meier survival curve analysis
²alpha <0.05

	TABLES 10-NL (c)

	Mean % survival (days post start)	Survival

RNAi

	0	1	2	3	4	5	6	7	8	analysis¹

gfp	100	92	84	78	72	62	58	56	48	−
diet only	100	84	72	68	64	58	52	42	42	−
NL014	100	86	68	60	46	32	24	18	14	+
NL018	100	82	70	54	40	30	18	14	12	+

	Chi squared	P value	Sig. Dif.²

diet versus:
NL014	8.088	0.0045	Yes
NL018	10.47	0.0012	Yes
gfp dsRNA versus:
NL014	14.55	0.0001	Yes
NL018	17.64	<0.0001	Yes
diet versus gfp dsRNA	0.6548	0.4184	No

¹= Data were analysed using Kaplan-Meier survival curve analysis
²alpha <0.05

	TABLES 10-NL (d)

	Mean % survival (days post start)	Survival

RNAi

	0	1	2	3	4	5	6	7	8	9	analysis¹

gfp	100	96	84	84	72	68	68	66	66	62	−
diet only	100	96	86	82	74	72	70	70	66	58	−
NL013	100	94	82	68	50	40	30	28	20	20	+
NL015	100	100	72	30	18	12	8	6	6	6	+
NL021	100	100	84	58	50	44	40	34	34	22	+

	Chi squared	P value	Sig. Dif.²

diet versus:
NL013	15.73	<0.0001	Yes
NL015	39.44	<0.0001	Yes
NL021	12.75	0.0004	Yes
gfp dsRNA versus:
NL013	16.42	<0.0001	Yes
NL015	39.15	<0.0001	Yes
NL021	14.1	0.0002	Yes
diet versus gfp dsRNA	0.1031	0.7481	No

¹= Data were analysed using Kaplan-Meier survival curve analysis
²alpha <0.05

	TABLE 11-NL

	Mean % survival (days post start)	Survival

NL002 RNAi

	0	1	2	3	4	5	6	7	analysis¹

diet only	100	100	96	90	86	78	78	78	−
1 μg/μl	100	84	80	44	26	8	6	6	+
0.2 μg/μl	100	84	60	12	8	4	2	2	+
0.08 μg/μl	100	84	62	18	14	6	6	6	+
0.04 μg/μl	100	84	48	24	22	22	22	22	+

	Chi squared	P value	Sig. Dif.²

diet versus:
NL002 1 μg/μl	57.53	<0.0001	Yes
NL002 0.2 μg/μl	74.54	<0.0001	Yes
NL002 0.08 μg/μl	64	<0.0001	Yes
NL002 0.04 μg/μl	39.49	<0.0001	Yes

¹= Data were analysed using Kaplan-Meier survival curve analysis
²alpha <0.05

Claims

1. An isolated nucleotide sequence comprising a nucleic acid sequence selected from the group comprising:

(i) sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591, 596, 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783, 788, 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878, 883, 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, 1066 to 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672, 1677, 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090, 2095, 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354, 2359, 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476, 2481 or 2486, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 49 to 158, 275 to 472, 533 to 575, 621 to 767, 813 to 862, 908 to 1040, 1161 to 1571, 1730 to 2039, 2120 to 2338, 2384 to 2460, or a complement thereof.

2. A double stranded ribonucleotide sequence produced from the expression of a polynucleotide sequence of claim 1, wherein ingestion of said double stranded ribonucleotide sequence by a plant insect pest inhibits the growth of said insect pest.

3. The double stranded ribonucleotide sequence of claim 2, wherein ingestion of said sequence inhibits expression of a nucleotide sequence substantially complementary to said sequence.

4. A cell transformed with a polynucleotide comprising a nucleic acid sequence as defined in claim 1, optionally operably linked to a regulatory sequence.

5. The cell of claim 4, wherein said cell is a plant cell.

6. A plant transformed with a polynucleotide having a nucleic acid sequence as defined in claim 1, said nucleic acid sequence optionally operably linked to a regulatory sequence.

7. The plant of claim 6, wherein said sequence inhibits a pest biological activity.

8. The plant of claim 6, wherein said sequence inhibits expression of a target sequence.

9. The plant of claim 8 wherein said target sequence is an insect, nematode or fungal sequence.

10. The plant of claim 6, wherein said plant is cytoplasmic male sterile.

11. The plant of claim 6, wherein said plant further comprises or expresses a pesticidal agent selected from the group consisting of a patatin, a Bacillus thuringiensis insecticidal protein, a Xenorhabdus insecticidal protein, a Photorhabdus insecticidal protein, a Bacillus laterosporous insecticidal protein, and a Bacillus sphearicus insecticidal protein.

12. The plant of claim 11 wherein said Bacillus thuringiensis insecticidal protein is selected from the group consisting of a Cry1, a Cry3, a TIC851, a CryET170, a Cry22, a binary insecticidal protein CryET33 and CryET34, a binary insecticidal protein CryET80 and CryET76, a binary insecticidal protein TIC100 and TIC101, and a binary insecticidal protein PS149B1.

13. The plant of claim 6, wherein said plant is chosen from the group comprising alfalfa, apple, apricot, artichoke, asparagus, avocado, banana, barley, beans, beet, blackberry, blueberry, broccoli, brussel sprouts, cabbage, canola, carrot, cassaya, cauliflower, a cereal, celery, cherry, citrus, clemintine, coffee, corn, cotton, cucumber, eggplant, endive, eucalyptus, figes, grape, grapefruit, groundnuts, ground cherry, kiwifruit, lettuce, leek, lemon, lime, pine, maize, mango, melon, millet, mushroom, nut aot, okra, onion, orange, an ornamental plant or flower or tree, papaya, parsley, pea, peach, peanut, peat, pepper, persimmon, pineapple, plantain, plum, pomegranate, potato, pumpkin, radicchio, radish, rapeseed, raspberry, rice, rye, sorghum, soy, soybean, spinach, strawberry, sugarbeet, sugarcane, sunflower, sweet potato, tangerine, tea, tobacco, tomato, a vine, watermelon, wheat, yams and zucchini.

14. The plant of claim 6, wherein said plant is resistant against infestation by an insect chosen from the group comprising Leptinotarsa spp. (e.g. L. decemlineata (Colorado potato beetle), L. juncta (false potato beetle), or L. texana (Texan false potato beetle)); Lema spp. (e.g. L. trilineata (three-lined potato beetle)); Epitrix spp. (e.g. E. cucumeris (potato flea beetle), E. hirtipennis (flea beetle), or E. tuberis (tuber flea beetle)); Epicauta spp. (e.g. E. vittata (striped blister beetle)); Epilachna spp. (e.g. E. varivetis (mexican bean beetle)); Phaedon spp. (e.g. P. cochleariae (mustard leaf beetle)); Nilaparvata spp. (e.g. N. lugens (brown planthopper)); Laodelphax spp. (e.g. L. striatellus (small brown planthopper)); Nephotettix spp. (e.g. N. virescens or N. cincticeps (green leafhopper), or N. nigropictus (rice leafhopper)); Sogatella spp. (e.g. S. furcifera (white-backed planthopper)); Acheta spp. (e.g. A. domesticus (house cricket)); Blissus spp. (e.g. B. leucopterus leucopterus (chinch bug)); Scotinophora spp. (e.g. S. vermidulate (rice blackbug)); Acrosternum spp. (e.g. A. hilare (green stink bug)); Parnara spp. (e.g. P. guttata (rice skipper)); Chilo spp. (e.g. C. suppressalis (rice striped stem borer), C. auricilius (gold-fringed stem borer), or C. polychrysus (dark-headed stem borer)); Chilotraea spp. (e.g. C. polychrysa (rice stalk borer)); Sesamia spp. (e.g. S. inferens (pink rice borer)); Tryporyza spp. (e.g. T. innotata (white rice borer), or T. incertulas (yellow rice borer)); Cnaphalocrocis spp. (e.g. C. medinalis (rice leafroller)); Agromyza spp. (e.g. A. oryzae (leafminer), or A. parvicornis (corn blot leafminer)); Diatraea spp. (e.g. D. saccharalis (sugarcane borer), or D. grandiosella (southwestern corn borer)); Narnaga spp. (e.g. N. aenescens (green rice caterpillar)); Xanthodes spp. (e.g. X. transversa (green caterpillar)); Spodoptera spp. (e.g. S. frugiperda (fall armyworm), S. exigua (beet armyworm), S. littoralis (climbing cutworm), or S. praefica (western yellowstriped armyworm)); Mythimna spp. (e.g. Mythmna (Pseudaletia) seperata (armyworm)); Helicoverpa spp. (e.g. H. zea (corn earworm)); Colaspis spp. (e.g. C. brunnea (grape colaspis)); Lissorhoptrus spp. (e.g. L. oryzophilus (rice water weevil)); Echinocnemus spp. (e.g. E. squamos (rice plant weevil)); Diclodispa spp. (e.g. D. armigera (rice hispa)); Oulema spp. (e.g. O. oryzae (leaf beetle); Sitophilus spp. (e.g. S. oryzae (rice weevil)); Pachydiplosis spp. (e.g. P. oryzae (rice gall midge)); Hydrellia spp. (e.g. H. griseola (small rice leafminer), or H. sasakii (rice stem maggot)); Chlorops spp. (e.g. C. oryzae (stem maggot)); Diabrotica spp. (e.g. D. virgifera virgifera (western corn rootworm), D. barberi (northern corn rootworm), D. undecimpunctata howardi (southern corn rootworm), D. virgifera zeae (Mexican corn rootworm); D. balteata (banded cucumber beetle)); Ostrinia spp. (e.g. O. nubilalis (European corn borer)); Agrotis spp. (e.g. A. ipsilon (black cutworm)); Elasmopalpus spp. (e.g. E. lignosellus (lesser cornstalk borer)); Melanotus spp. (wireworms); Cyclocephala spp. (e.g. C. borealis (northern masked chafer), or C. immaculata (southern masked chafer)); Popillia spp. (e.g. P. japonica (Japanese beetle)); Chaetocnema spp. (e.g. C. pulicaria (corn flea beetle)); Sphenophorus spp. (e.g. S. maidis (maize billbug)); Rhopalosiphum spp. (e.g. R. maidis (corn leaf aphid)); Anuraphis spp. (e.g. A. maidiradicis (corn root aphid)); Melanoplus spp. (e.g. M. femurrubrum (redlegged grasshopper) M. differentialis (differential grasshopper) or M. sanguinipes (migratory grasshopper)); Hylemya spp. (e.g. H. platura (seedcorn maggot)); Anaphothrips spp. (e.g. A. obscrurus (grass thrips)); Solenopsis spp. (e.g. S. milesta (thief ant)); or spp. (e.g. T. urticae (twospotted spider mite), T. cinnabarinus (carmine spider mite); Helicoverpa spp. (e.g. H. zea (cotton bollworm), or H. armigera (American bollworm)); Pectinophora spp. (e.g. P. gossypiella (pink bollworm)); Earias spp. (e.g. E. vittella (spotted bollworm)); Heliothis spp. (e.g. H. virescens (tobacco budworm)); Anthonomus spp. (e.g. A. grandis (boll weevil)); Pseudatomoscelis spp. (e.g. P. seriatus (cotton fleahopper)); Trialeurodes spp. (e.g. T. abutiloneus (banded-winged whitefly) T. vaporariorum (greenhouse whitefly)); Bemisia spp. (e.g. B. argentifolii (silverleaf whitefly)); Aphis spp. (e.g. A. gossypii (cotton aphid)); Lygus spp. (e.g. L. lineolaris (tarnished plant bug) or L. hesperus (western tarnished plant bug)); Euschistus spp. (e.g. E. conspersus (consperse stink bug)); Chlorochroa spp. (e.g. C. sayi (Say stinkbug)); Nezara spp. (e.g. N. viridula (southern green stinkbug)); Thrips spp. (e.g. T. tabaci (onion thrips)); Frankliniella spp. (e.g. F. fusca (tobacco thrips), or F. occidentalis (western flower thrips)); Empoasca spp. (e.g. E. fabae (potato leafhopper)); Myzus spp. (e.g. M. persicae (green peach aphid)); Paratrioza spp. (e.g. P. cockerelli (psyllid)); Conoderus spp. (e.g. C. falli (southern potato wireworm), or C. vespertinus (tobacco wireworm)); Phthorimaea spp. (e.g. P. operculella (potato tuberworm)); Macrosiphum spp. (e.g. M. euphorbiae (potato aphid)); Thyanta spp. (e.g. T. pallidovirens (redshouldered stinkbug)); Phthorimaea spp. (e.g. P. operculella (potato tuberworm)); Helicoverpa spp. (e.g. H. zea (tomato fruitworm); Keiferia spp. (e.g. K. lycopersicella (tomato pinworm)); Limonius spp. (wireworms); Manduca spp. (e.g. M. sexta (tobacco hornworm), or M. quinquemaculata (tomato hornworm)); Liriomyza spp. (e.g. L. sativae, L. trifolli or L. huidobrensis (leafminer)); Drosophilla spp. (e.g. D. melanogaster, D. yakuba, D. pseudoobscura or D. simulans); Carabus spp. (e.g. C. granulatus); Chironomus spp. (e.g. C. tentanus); Ctenocephalides spp. (e.g. C. felis (cat flea)); Diaprepes spp. (e.g. D. abbreviatus (root weevil)); Ips spp. (e.g. I. pini (pine engraver)); Tribolium spp. (e.g. T. castaneum (red floor beetle)); Glossina spp. (e.g. G. morsitans (tsetse fly)); Anopheles spp. (e.g. A. gambiae (malaria mosquito)); Helicoverpa spp. (e.g. H. armigera (African Bollworm)); Acyrthosiphon spp. (e.g. A. pisum (pea aphid)); Apis spp. (e.g. A. melifera (honey bee)); Homalodisca spp. (e.g. H. coagulate (glassy-winged sharpshooter)); Aedes spp. (e.g. Ae. aegypti (yellow fever mosquito)); Bombyx spp. (e.g. B. mori (silkworm)); Locusta spp. (e.g. L. migratoria (migratory locust)); Boophilus spp. (e.g. B. microplus (cattle tick)); Acanthoscurria spp. (e.g. A. gomesiana (red-haired chololate bird eater)); Diploptera spp. (e.g. D. punctata (pacific beetle cockroach)); Heliconius spp. (e.g. H. erato (red passion flower butterfly) or H. melpomene (postman butterfly)); Curculio spp. (e.g. C. glandium (acorn weevil)); Plutella spp. (e.g. P. xylostella (diamondback moth)); Amblyomma spp. (e.g. A. variegatum (cattle tick)); Anteraea spp. (e.g. A. yamamai (silkmoth)); and Armigeres spp. (e.g. A. subalbatus).

15. A seed or reproductive or propagation material for a plant of claim 6, wherein said seed or reproductive or propagation material comprises said polynucleotide or wherein said seed comprises a double stranded ribonucleotide sequence produced from the expression of said polynucleotide.

16. A product produced from the plant of claim 6, wherein said product comprises said polynucleotide or a double stranded ribonucleotide sequence produced from the expression of said polynucleotide.

17. The product of claim 16, wherein said product is selected from the group consisting of food, feed, fiber, paper, meal, protein, starch, flour, silage, coffee, tea, and oil.

18. A pesticide comprising the product of claim 16, said product expressing said nucleic acid sequence.

19. A method for controlling or preventing insect growth comprising providing an insect pest with the product of claim 16, wherein said product comprises a polynucleotide sequence that inhibits an insect biological activity.

20. The method of claim 19, wherein said polynucleotide comprises said nucleic acid sequence as defined in claim 1.

21. A method for producing a plant resistant against a plant pathogenic organism comprising:

transforming a plant cell with a polynucleotide having a nucleic acid sequence as defined in claim 1, said nucleic acid sequence optionally operably linked to a regulatory sequence,

regenerating a plant from the transformed plant cell; and

growing the transformed plant under conditions suitable for the expression of an RNA molecule from said polynucleotide, said grown transformed plant resistant to said plant pathogenic organism compared to an untransformed plant.

22. A method for improving yield, comprising:

regenerating a plant from the transformed plant cell; and

growing the transformed plant under conditions suitable for the expression of an RNA molecule from said polynucleotide, said expression inhibiting feeding by a plant pathogenic organism and loss of yield due to pest infestation.

23. The method according to claim 50, wherein polynucleotide expression produces an RNA molecule that suppresses a target gene in an insect pest that has ingested a portion of said crop plant, wherein said target gene performs at least one essential function selected from the group consisting of feeding by the pest, viability of the pest, pest cell apoptosis, differentiation and development of the pest or any pest cell, sexual reproduction by the pest, muscle formation, muscle twitching, muscle contraction, juvenile hormone formation and/or reduction, juvenile hormone regulation, ion regulation and transport, maintenance of cell membrane potential, amino acid biosynthesis, amino acid degradation, sperm formation, pheromone synthesis, pheromone sensing, antennae formation, wing formation, leg formation, egg formation, larval maturation, digestive enzyme formation, haemolymph synthesis, haemolymph maintenance, neurotransmission, larval stage transition, pupation, emergence from pupation, cell division, energy metabolism, respiration, cytoskeletal structure synthesis and maintenance, nucleotide metabolism, nitrogen metabolism, water use, water retention, and sensory perception.

24. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160 to 163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 246, or 2486, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160 to 163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 246, or 2486, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160 to 163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 246, or 2486, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 49 to 158, or the complement thereof, and

the insect is chosen from the group comprising Leptinotarsa spp. (e.g. L. decemlineata (Colorado potato beetle), L. juncta (false potato beetle), and L. texana (Texan false potato beetle)).

25. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 512, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 512, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 247, 249, 251, 253, 255, 257, 259, 275 to 472, 473, 478, 483, 488, 493, 498, 503, 508 to 512, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 275 to 472, or the complement thereof, and

the insect is chosen from the group comprising Phaedon spp. (e.g. P. cochleariae (mustard leaf beetle)).

26. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591 or 596, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591 or 596, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 513, 515, 517, 519, 521, 533 to 575, 576, 581, 586, 591 or 596, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 533 to 575, or the complement thereof, and

the insect is chosen from the group comprising Epilachna spp. (e.g. E. varivetis (mexican bean beetle)).

27. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783 or 788, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783 or 788, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 601, 603, 605, 607, 609, 621 to 767, 768, 773, 778, 783 or 788, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 621 to 767, or the complement thereof, and

the insect is chosen from the group comprising Anthonomus spp. (e.g. A. grandis (boll weevil)).

28. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878 or 883, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878 or 883, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 793, 795, 797, 799, 801, 813 to 862, 863, 868, 873, 878 or 883, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 813 to 862, or the complement thereof, and

the insect is chosen from the group comprising Tribolium spp. (e.g. T. castaneum (red floor beetle)).

29. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, or 1066 to 1070, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, or 1066 to 1070, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, or 1066 to 1070, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 908 to 1040, or the complement thereof, and

the insect is chosen from the group comprising Myzus spp. (e.g. M. persicae (green peach aphid)).

30. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672 or 1677, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672 or 1677, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 1071, 1073, 1075, 1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103, 1105, 1107, 1109, 1111, 1113, 1161 to 1571, 1572, 1577, 1582, 1587, 1592, 1597, 1602, 1607, 1612, 1617, 1622, 1627, 1632, 1637, 1642, 1647, 1652, 1657, 1662, 1667, 1672 or 1677, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1161 to 1571, or the complement thereof, and

the insect is chosen from the group comprising Nilaparvata spp. (e.g. N. lugens (brown planthopper)).

31. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090 or 2095, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090 or 2095, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 1682, 1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1730 to 2039, 2040, 2045, 2050, 2055, 2060, 2065, 2070, 2075, 2080, 2085, 2090 or 2095, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1730 to 2039, or the complement thereof, and

the insect is chosen from the group comprising Chilo spp. (e.g. C. suppressalis (rice striped stem borer), C. auricilius (gold-fringed stem borer), or C. polychysus (dark-headed stem borer)).

32. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354 or 2359, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354 or 2359, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 2100, 2102, 2104, 2106, 2108, 2120 to 2338, 2339, 2344, 2349, 2354 or 2359, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 2120 to 2338, or the complement thereof, and

the insect is chosen from the group comprising Plutella spp. (e.g. P. xylostella (diamondback moth)).

33. The method according to claim 50 wherein:

the nucleic acid sequence is chosen from the group comprising:

(i) sequences represented by any of SEQ ID NOs 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 2364, 2366, 2368, 2370, 2372, 2384 to 2460, 2461, 2466, 2471, 2476 or 2481, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 2384 to 2460, or the complement thereof, and

the insect is chosen from the group comprising Acheta spp. (e.g. A. domesticus (house cricket)).

34. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

(i) sequences represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 246, or 2486, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 246, or 2486, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 49 to 158, 159, 160-163, 168, 173, 178, 183, 188, 193, 198, 203, 208, 215, 220, 225, 230, 240 to 246, or 2486, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 49 to 158, or the complement thereof.

35. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 275 to 472, or the complement thereof.

36. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 533 to 575, or the complement thereof.

37. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 621 to 767, or the complement thereof.

38. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 813 to 862, or the complement thereof.

39. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

(i) sequences represented by any of SEQ ID NOs 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, or 1066 to 1071, or the complement thereof,

(ii) sequences which are at least 70%, preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%, 96%, 97%, 98% or 99% identical to a sequence represented by any of SEQ ID NOs 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, or 1066 to 1071, or the complement thereof, and

(iii) sequences comprising at least 17 contiguous nucleotides of any of the sequences represented by SEQ ID NOs 888, 890, 892, 894, 896, 908 to 1040, 1041, 1046, 1051, 1056, 1061, or 1066 to 1071, or the complement thereof,

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 908 to 1040, or the complement thereof.

40. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1161 to 1571, or the complement thereof.

41. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 1730 to 2039, or the complement thereof.

42. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 2120 to 2338, or the complement thereof.

43. A transgenic plant resistant to an insect pest comprising a polynucleotide comprising a nucleic acid sequence selected from the group comprising:

or wherein said nucleic acid sequence is an orthologue of a gene comprising at least 17 contiguous nucleotides of any of SEQ ID NOs 2384 to 2460, or the complement thereof.

44. The transgenic plant according to claim 34 further comprising or expressing a pesticidal agent selected from the group consisting of a patatin, a Bacillus thuringiensis insecticidal protein, a Xenorhabdus insecticidal protein, a Photorhabdus insecticidal protein, a Bacillus laterosporous insecticidal protein, and a Bacillus sphearicus insecticidal protein.

45. The transgenic plant of claim 44 wherein said Bacillus thuringiensis insecticidal protein is selected from the group consisting of a Cry1, a Cry3, a TIC851, a CryET170, a Cry22, a binary insecticidal protein CryET33 and CryET34, a binary insecticidal protein CryET80 and CryET76, a binary insecticidal protein TIC100 and TIC101, and a binary insecticidal protein PS149B1.

46-48. (canceled)

49. A pesticide comprising a plant of claim 6 or reproductive or propagation material thereof, said plant or reproductive or propagation material thereof expressing said nucleic acid sequence.

50. A method for controlling or preventing insect growth comprising providing an insect pest with the plant of claim 6 or reproductive or propagation material thereof, wherein said plant reproductive or propagation material thereof comprises a sequence that inhibits an insect biological activity.

51. A method of claim 50, wherein said polynucleotide comprises said nucleic acid.