US20060115819A1 - Detection, identification and differentiation of eubacterial taxa using a hybridization assay - Google Patents

Detection, identification and differentiation of eubacterial taxa using a hybridization assay Download PDF

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Publication number
US20060115819A1
US20060115819A1 US10/537,787 US53778705A US2006115819A1 US 20060115819 A1 US20060115819 A1 US 20060115819A1 US 53778705 A US53778705 A US 53778705A US 2006115819 A1 US2006115819 A1 US 2006115819A1
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US
United States
Prior art keywords
seq
staphylococcus
probes
nucleic acid
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/537,787
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English (en)
Inventor
Sofie Claeys
Geert Jannes
Gerd Haberhausen
Thomas Emrich
Lia Verdoodt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Europe NV SA
Roche Diagnostics GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Assigned to ROCHE DIAGNOSTICS GMBH, INNOGENETICS N.V. reassignment ROCHE DIAGNOSTICS GMBH ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: EMRICH, THOMAS, HABERHAUSEN, GERD, CLAEYS, SOFIE, JANNES, GEERT, VERDOODT, LIA
Publication of US20060115819A1 publication Critical patent/US20060115819A1/en
Priority to US12/105,380 priority Critical patent/US8945850B2/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Definitions

  • the present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus , in a biological sample.
  • rRNA ribosomal ribonucleic acid
  • An aspect of the present invention relates to new polynucleotides for use as probes and/or primers, which have as target a particular region of the 16S-23S rRNA spacer region of Staphylococcus aureus , and which allow the detection and/or identification of Staphylococcus species, in particular of Staphylococcus aureus.
  • Another object of the present invention is a kit, in which said probes and/or primers are used, for the detection and/or identification Staphylococcus species, in particular of Staphylococcus aureus.
  • Preferred primers of the invention are single stranded DNA polynucleotides capable of acting as a point of initiation for synthesis of the target sequence of the invention.
  • the length and the sequence of a primer of the invention must be such that they allow to prime the synthesis of the extension products.
  • Another object of the invention relates to sets of 2 polynucleotide probes, also referred to as HybProbes, both HybProbes hybridizing to the same target sequence, adjacent to each other, with no more than 25 nucleotides between said 2 HybProbes, preferably with no more than 10 nucleotides, in particular with no more than 5 nucleotides.
  • the fluorescence may be measured during the amplification step, generating then amplification curves, or after the amplification step, for a melting curve analysis generating melting curves.
  • the ability to simultaneously generate hybridization results with a large number of probes is another benefit of the LiPA technology.
  • the amount of information which can be obtained by a particular combination of probes greatly outnumbers the data obtained by using single probe assays. Therefore the selection of probes on the membrane strip is of utmost importance since an optimized set of probes will generate the maximum of information possible.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
US10/537,787 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay Abandoned US20060115819A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/105,380 US8945850B2 (en) 2002-12-06 2008-04-18 Detection, identification and differentiation of Staphylococcus using a hybridization assay

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02447247.4 2002-12-06
EP02447247 2002-12-06
PCT/EP2003/013907 WO2004052606A2 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/105,380 Continuation US8945850B2 (en) 2002-12-06 2008-04-18 Detection, identification and differentiation of Staphylococcus using a hybridization assay

Publications (1)

Publication Number Publication Date
US20060115819A1 true US20060115819A1 (en) 2006-06-01

Family

ID=32479868

Family Applications (2)

Application Number Title Priority Date Filing Date
US10/537,787 Abandoned US20060115819A1 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
US12/105,380 Expired - Fee Related US8945850B2 (en) 2002-12-06 2008-04-18 Detection, identification and differentiation of Staphylococcus using a hybridization assay

Family Applications After (1)

Application Number Title Priority Date Filing Date
US12/105,380 Expired - Fee Related US8945850B2 (en) 2002-12-06 2008-04-18 Detection, identification and differentiation of Staphylococcus using a hybridization assay

Country Status (11)

Country Link
US (2) US20060115819A1 (de)
EP (1) EP1567668B1 (de)
JP (2) JP2006508671A (de)
AT (1) ATE430209T1 (de)
AU (1) AU2003289984A1 (de)
CA (1) CA2504911C (de)
DE (1) DE60327462D1 (de)
DK (1) DK1567668T3 (de)
ES (1) ES2326316T3 (de)
HK (1) HK1085159A1 (de)
WO (1) WO2004052606A2 (de)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080199878A1 (en) * 2002-12-06 2008-08-21 Innogenetics N.V. Detection, identification and differentiation of eubacterial taxa using a hybridization assay
US20090081663A1 (en) * 2007-04-19 2009-03-26 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
KR101065904B1 (ko) * 2003-09-02 2011-09-19 소니 에릭슨 모빌 커뮤니케이션즈 에이비 두 메모리 사이의 보안성 데이터 전송

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2393302T3 (es) * 2004-09-30 2012-12-20 Innogenetics N.V. Detección, identificación y diferenciación de la especie serratia utilizando la región intergénica
JP4967095B2 (ja) * 2005-08-12 2012-07-04 財団法人ヒューマンサイエンス振興財団 黄色ブドウ球菌濃度測定方法及び食中毒可能性判定方法
US20090286691A1 (en) * 2006-01-20 2009-11-19 Genein Co., Ltd. Oligonucleotide for Detection of Bacteria Associated with Sepsis and Microarrays and Method for Detection of the Bacteria Using the Oligonucleotide
EP2126138A4 (de) * 2007-01-08 2011-01-19 Medigenes Co Ltd Dna-chip zum nachweis von staphylococcus aureus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849488A (en) * 1996-02-27 1998-12-15 Oulutech Ltd. DNA-sequence-based diagnosis of mastitis from a milk sample
US6312903B1 (en) * 1994-06-24 2001-11-06 Innogenetics N.V. Simulataneous detection, identification and differentiation of eubacterial taxa using a hybridization assay

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6737248B2 (en) * 1996-01-05 2004-05-18 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences
JP3844996B2 (ja) * 2000-11-15 2006-11-15 エフ．ホフマン−ラ ロシュ アーゲー 反復性ｐｃｒ産物の融解曲線解析方法
EP1567668B1 (de) * 2002-12-06 2009-04-29 Innogenetics N.V. Nachweis, identifizierung und differenzierung von eubakterien unter verwendung eines hybridisierungs-assays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312903B1 (en) * 1994-06-24 2001-11-06 Innogenetics N.V. Simulataneous detection, identification and differentiation of eubacterial taxa using a hybridization assay
US5849488A (en) * 1996-02-27 1998-12-15 Oulutech Ltd. DNA-sequence-based diagnosis of mastitis from a milk sample

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080199878A1 (en) * 2002-12-06 2008-08-21 Innogenetics N.V. Detection, identification and differentiation of eubacterial taxa using a hybridization assay
US8945850B2 (en) 2002-12-06 2015-02-03 Fujirebio Europe N.V. Detection, identification and differentiation of Staphylococcus using a hybridization assay
KR101065904B1 (ko) * 2003-09-02 2011-09-19 소니 에릭슨 모빌 커뮤니케이션즈 에이비 두 메모리 사이의 보안성 데이터 전송
US20090081663A1 (en) * 2007-04-19 2009-03-26 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US20100255490A1 (en) * 2007-04-19 2010-10-07 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US20110151466A1 (en) * 2007-04-19 2011-06-23 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8017337B2 (en) 2007-04-19 2011-09-13 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8362228B2 (en) 2007-04-19 2013-01-29 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8512954B2 (en) 2007-04-19 2013-08-20 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8557974B2 (en) 2007-04-19 2013-10-15 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8557524B2 (en) 2007-04-19 2013-10-15 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US9074260B2 (en) 2007-04-19 2015-07-07 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria

Also Published As

Publication number Publication date
JP2010088447A (ja) 2010-04-22
JP2006508671A (ja) 2006-03-16
AU2003289984A1 (en) 2004-06-30
EP1567668B1 (de) 2009-04-29
WO2004052606A3 (en) 2004-07-29
ES2326316T3 (es) 2009-10-07
EP1567668A2 (de) 2005-08-31
DE60327462D1 (de) 2009-06-10
AU2003289984A8 (en) 2004-06-30
ATE430209T1 (de) 2009-05-15
HK1085159A1 (en) 2006-08-18
CA2504911A1 (en) 2004-06-24
JP5156726B2 (ja) 2013-03-06
CA2504911C (en) 2015-08-11
US8945850B2 (en) 2015-02-03
US20080199878A1 (en) 2008-08-21
DK1567668T3 (da) 2009-08-17
WO2004052606A2 (en) 2004-06-24

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Legal Events

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AS Assignment

Owner name: INNOGENETICS N.V., BELGIUM

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:CLAEYS, SOFIE;JANNES, GEERT;HABERHAUSEN, GERD;AND OTHERS;REEL/FRAME:017396/0252;SIGNING DATES FROM 20050426 TO 20050511

Owner name: ROCHE DIAGNOSTICS GMBH, GERMANY

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:CLAEYS, SOFIE;JANNES, GEERT;HABERHAUSEN, GERD;AND OTHERS;REEL/FRAME:017396/0252;SIGNING DATES FROM 20050426 TO 20050511

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION