US20060065544A1 - Process for preducing mixed electrolyzed water - Google Patents
Process for preducing mixed electrolyzed water Download PDFInfo
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- US20060065544A1 US20060065544A1 US10/545,341 US54534105A US2006065544A1 US 20060065544 A1 US20060065544 A1 US 20060065544A1 US 54534105 A US54534105 A US 54534105A US 2006065544 A1 US2006065544 A1 US 2006065544A1
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- water
- aqueous solution
- organic electrolyte
- electrolysis
- electrolyzed water
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 89
- 238000000034 method Methods 0.000 title description 3
- 230000008569 process Effects 0.000 title description 2
- 238000005868 electrolysis reaction Methods 0.000 claims abstract description 55
- 239000005486 organic electrolyte Substances 0.000 claims abstract description 45
- 239000007864 aqueous solution Substances 0.000 claims abstract description 44
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 claims abstract description 30
- 239000003643 water by type Substances 0.000 claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 15
- 238000004448 titration Methods 0.000 claims abstract description 11
- 238000007323 disproportionation reaction Methods 0.000 claims abstract description 8
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 7
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 85
- 235000010323 ascorbic acid Nutrition 0.000 claims description 43
- 239000011668 ascorbic acid Substances 0.000 claims description 43
- 229960005070 ascorbic acid Drugs 0.000 claims description 42
- 229930003935 flavonoid Natural products 0.000 claims description 7
- 235000017173 flavonoids Nutrition 0.000 claims description 7
- 150000002215 flavonoids Chemical class 0.000 claims description 7
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims description 6
- 235000005487 catechin Nutrition 0.000 claims description 6
- 150000001765 catechin Chemical class 0.000 claims description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 4
- 235000013824 polyphenols Nutrition 0.000 claims description 4
- 235000008216 herbs Nutrition 0.000 claims description 3
- 230000002427 irreversible effect Effects 0.000 claims description 2
- 238000010494 dissociation reaction Methods 0.000 description 30
- 230000005593 dissociations Effects 0.000 description 30
- 239000003792 electrolyte Substances 0.000 description 18
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 9
- 239000001301 oxygen Substances 0.000 description 9
- 229910052760 oxygen Inorganic materials 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- 239000008151 electrolyte solution Substances 0.000 description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 150000002500 ions Chemical class 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 235000002639 sodium chloride Nutrition 0.000 description 4
- -1 MDA Chemical compound 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 2
- SBJKKFFYIZUCET-JLAZNSOCSA-N Dehydro-L-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-JLAZNSOCSA-N 0.000 description 2
- 238000004435 EPR spectroscopy Methods 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 229910001260 Pt alloy Inorganic materials 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 229940072107 ascorbate Drugs 0.000 description 2
- 238000006701 autoxidation reaction Methods 0.000 description 2
- 239000012267 brine Substances 0.000 description 2
- 229950001002 cianidanol Drugs 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000005875 quercetin Nutrition 0.000 description 2
- 229960001285 quercetin Drugs 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 2
- 238000010408 sweeping Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- GJQWCDSAOUMKSE-STHAYSLISA-N 2,3-diketogulonic acid Chemical compound OC[C@H](O)[C@@H](O)C(=O)C(=O)C(O)=O GJQWCDSAOUMKSE-STHAYSLISA-N 0.000 description 1
- ZLZUSTRGWUZODW-UHFFFAOYSA-O C.C.C.C.[H+].[H+].[H]C(O)(CO)C1OC(=O)C(O)=C1[O-] Chemical compound C.C.C.C.[H+].[H+].[H]C(O)(CO)C1OC(=O)C(O)=C1[O-] ZLZUSTRGWUZODW-UHFFFAOYSA-O 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- SBJKKFFYIZUCET-UHFFFAOYSA-N Dehydroascorbic acid Natural products OCC(O)C1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-UHFFFAOYSA-N 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- HENCIVUQNZJCAI-UHFFFAOYSA-N O=C1C2=C(C=C(O)C=C2O)OC(C2=CC(O)=C(O)C=C2)C1O.OC1=CC2=C(CC(O)C(C3=CC(O)=C(O)C=C3)O2)C(O)=C1 Chemical compound O=C1C2=C(C=C(O)C=C2O)OC(C2=CC(O)=C(O)C=C2)C1O.OC1=CC2=C(CC(O)C(C3=CC(O)=C(O)C=C3)O2)C(O)=C1 HENCIVUQNZJCAI-UHFFFAOYSA-N 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910001514 alkali metal chloride Inorganic materials 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 125000003289 ascorbyl group Chemical group [H]O[C@@]([H])(C([H])([H])O*)[C@@]1([H])OC(=O)C(O*)=C1O* 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 235000020960 dehydroascorbic acid Nutrition 0.000 description 1
- 239000011615 dehydroascorbic acid Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000007772 electrode material Substances 0.000 description 1
- 238000003411 electrode reaction Methods 0.000 description 1
- 230000005592 electrolytic dissociation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 235000007352 monodehydroascorbic acid Nutrition 0.000 description 1
- 239000011744 monodehydroascorbic acid Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
- 235000020681 well water Nutrition 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/467—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis by electrochemical disinfection; by electrooxydation or by electroreduction
- C02F1/4672—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis by electrochemical disinfection; by electrooxydation or by electroreduction by electrooxydation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/46104—Devices therefor; Their operating or servicing
- C02F1/46109—Electrodes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/467—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis by electrochemical disinfection; by electrooxydation or by electroreduction
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/02—Non-contaminated water, e.g. for industrial water supply
- C02F2103/026—Treating water for medical or cosmetic purposes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2201/00—Apparatus for treatment of water, waste water or sewage
- C02F2201/46—Apparatus for electrochemical processes
- C02F2201/461—Electrolysis apparatus
- C02F2201/46105—Details relating to the electrolytic devices
- C02F2201/4618—Supplying or removing reactants or electrolyte
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/04—Oxidation reduction potential [ORP]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/05—Conductivity or salinity
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
Definitions
- This invention relates to a process for preparing a mixed electrolyzed water consisting of an anodic and a cathodic electrolyzed waters, which is capable of dismutating superoxide radical.
- this invention relates to a process for preparing a mixed electrolyzed water capable of dismutating superoxide radical, comprising electrolyzing an aqueous organic electrolyte solution containing virtually only an organic water-soluble electrolyte such a ascorbic acid as an electrolyte.
- aqueous dilute electrolyte solution of an alkali-metal chloride is electrolyzed, followed by removing an anodic electrolyzed water (acidic water) with a lower pH generated in the anodic side, which can be utilized for sterilization or disinfection.
- anodic electrolyzed water acidic water
- Examples of a diaphragm used include a charged membrane which is an ion-exchange resin film and a non-charged membrane having a microporous structure.
- hypochlorous acid In an anodic electrolyzed water, hypochlorous acid is generated.
- an anodic electrolyzed water is used for sterilization or disinfection.
- Such utilization is prevalent in medical institutions and so on.
- small amounts of ozone and dissolved oxygen present in the acidic water promotes granulation, it has been studied as an aid for surgical treatment.
- a cathodic electrolyzed water (alkaline water) in the cathodic side can be generated by electrolyzing tap water in place of a dilute electrolytic solution, and has been used for drinking. It has been also reported that to the aqueous electrolytic solution, an organic acid such as ascorbic acid and gallic acid is added, not as an electrolytic aid, but as an additive.
- ascorbic acid is used in the presence of an electrolytic aid. Ascorbic acid is added for controlling pH of a cathodic electrolyzed water and for removing free chlorine in an anodic electrolyzed water.
- Ascorbic acid has OH groups at 2- and 3-positions.
- the OH at 3-position is dissociated into —O ⁇ and H + , having acidity.
- the OH at 2-position is dissociated into —O ⁇ and H + , its dissociation ratio is too low to allow ascorbic acid to be used as an electrolytic aid.
- An electrolysis mechanism for an aqueous solution of ascorbic acid is so complicated that intermediates have not been identified, but the mechanism is observed a redox reaction in principle.
- Ascorbic acid generally undergoes autoxidation in the following manner.
- AsA, MDA, DHA and DKG represent ascorbic acid, monodehydroascorbic acid, dehydroascorbic acid and 2,3-diketogulonic acid, respectively.
- ascorbic acid can dismutate and eliminate superoxide radical which is generally known as “active oxygen”. Therefore, ascorbic acid has drawn attention as an antioxidant.
- Dismutation is a reaction where superoxide radical is decomposed and hydrogen peroxide generates as shown in equation (2): O ⁇ 2 ⁇ +O ⁇ 2 ⁇ +2H + ⁇ H 2 O 2 +O 2 (2)
- ascorbic acid is a useful compound for eliminating superoxide radical.
- the mixed electrolyzed water thus obtained has a lower DO and improved ability to dismutate superoxide radical.
- the mixed electrolyzed water can be, therefore, conveniently used in a variety of applications such as sterilization, disinfection, granulation, health maintenance and cosmetic applications.
- an anodic and a cathodic electrolyzed waters are prepared as a mixture in this preparation process, it requires a simpler manufacturing apparatus in comparison with a conventional process where an electrolyzed water in one electrode side is taken out.
- this invention employs a vitamin, ascorbic acid whose safety to a human body has been established, the cathodic electrolyzed water prepared using the vitamin as an electrolysis aid is also quite safe.
- a process for preparing a mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters comprising the step of electrolyzing an aqueous solution of an organic electrolyte containing a water-soluble inorganic salt in less than 0.1 mM and an organic electrolyte in 1 to 50 mM which is fed into a non-diaphragm electrolytic bath comprising at least a pair of inactive electrodes separated from each other by an inter-electrode distance of 2 mm or less, wherein the aqueous solution of an organic electrolyte with pH equal to that of the mixed electrolyzed water prepared by electrolysis is neutralized with a titration volume less than that for the raw aqueous solution in neutralization titration with an aqueous solution of sodium hydroxide or has a higher dismutating activity to superoxide radical per mole than the raw aqueous solution.
- an inorganic electrolyte such as sodium chloride, potassium chloride, calcium chloride and sodium sulfate is ionized, i.e., completely dissociated into a cation and an anion.
- an inorganic electrolyte such as sodium chloride, potassium chloride, calcium chloride and sodium sulfate
- Water dissolves and ionizes an electrolyte, i.e., a substance comprised of electrically positive and negative elements, and loosens association between molecules comprised of a positive and a negative elements.
- electrolytes inherently has lower freedom. Such an electrolyte may not be, although being dissolved to some degree, completely dissolved in water and some part of the electrolyte remains insoluble. Examples of a measure of freedom in an aqueous solution include a dissociation constant, a dissociation index and an electrolytic dissociation constant.
- a dissociation constant is defined as below.
- ABA + +B ⁇ (3) [ A + ] [B ⁇ ]/[AB] Ka (4)
- a dissociation ratio herein is called a dissociation constant, Ka, provided that in terms of conditions, a temperature and a pressure must be constant; specifically 25° C. and 1 atm, respectively, in a standard condition.
- a dissociation constant of a water-soluble electrolyte indicates a degree of its dissociation.
- This reaction proceeds in a strong electric-field layer in the electrode surface, so that the reaction is initiated by an extremely minute electric energy.
- Relative dissociation rates may be compared by neutralization titration of the raw aqueous solution before electrolysis and the resulting solution after electrolysis with an acid or base, followed by determination of a difference between the titration volumes. A smaller titration volume indicates a larger dissociation in an aqueous solution.
- AsA L-ascorbic acid
- AsA is dissociated in two dissociation sites as shown in formula (8).
- reactive sites are essentially OH groups attached at 2- and 3-positions, which are dissociated into H + and —O ⁇ .
- a dissociation index of 4.25 indicates that the OH group is dissociated into a positive and a negative ions in an extremely small amount of (1 ⁇ 2) ⁇ 10 ⁇ 4.25 M (2.8117 ⁇ 10 ⁇ 5 M) in an aqueous solution up to one liter at 1 atm and 25° C.
- a low-dissociating water-soluble substance can be dissolved in an electrolyzed water with improved dissociating ability, to improve dissociation of the substance, resulting in making the low-dissociating water-soluble substance more reactive.
- ascorbic acid has dissociable groups (—OH group) at 2- and 3-positions, which have dissociation indices, pKa, of 11.79 and 4.25, respectively, at 25° C. and 1 atm.
- pKa dissociation indices
- pKa dissociation indices
- a mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters prepared by electrolysis of a dilute aqueous solution of ascorbic acid contains no electrolyte ions other than ascorbate ions.
- the OH group at 3-position is, therefore, not used in neutralization of an alkali generated in the cathode, maintaining its reducibility.
- an anodic and a cathodic electrolyzed waters may be easily mixed.
- dissolved oxygen generated by anodic oxidation of water reacts with dissolved hydrogen generated by cathodic reduction of water, to give water.
- polarity of an electrolysis voltage can be alternated to further accelerate mixing of the anodic and the cathodic electrolyzed waters. Consequently, a concentration of dissolved oxygen generated by oxidation of water in the anode becomes 1 mg/L or less because the dissolved oxygen is consumed by AsA and reducing dissolved hydrogen.
- a dissociation ratio can be compared by a neutralization volume for a substance such as AsA which exhibits acidity by a 2,3-ene diol, while the dissociation ratio can be compared by determining dismutating ability for superoxide radical to a substance which does not exhibit acidity or basicity.
- water-soluble polyphenols, water-soluble flavonoids and catechins exhibit dismutating ability to superoxide radical.
- AsA proton from an OH group attached to a carbon atom having a double bond exhibits dismutating ability.
- a flavonoid a proton from the OH group exhibits antioxidation ability as shown in equation (9). O 2 ⁇ ⁇ +2H + ⁇ H 2 O 2 (9)
- quercetine and catechin are shown in formulas (10) and (11).
- FIG. 1 schematically shows a configuration of an apparatus for preparing a mixed electrolyzed water used in a process for preparing a mixed electrolyzed water of this invention.
- FIG. 2 schematically shows another configuration of an apparatus for preparing a mixed electrolyzed water used in a process for preparing a mixed electrolyzed water of this invention.
- FIG. 3 is a graph showing relationship between an electrolysis time for a mixed electrolyzed water in Example 1 and a neutralization volume of a 0.1 N aqueous solution of NaOH.
- FIG. 4 is a graph showing a relationship between an electrolysis time for a mixed electrolyzed water prepared by electrolyzing an aqueous solution of an organic electrolyte and a dismutating ability to superoxide radical.
- 2 raw electrolysis water reservoir
- 4 aqueous solution of an organic electrolyte
- 6 pump
- 8 feeding line for an aqueous solution of an organic electrolyte
- 10 non-diaphragm electrolytic bath
- 12 , 14 electrode
- 16 power source for electrolysis
- 18 , 20 interconnections
- 22 drain line for a mixed electrolyzed water
- 30 non-diaphragm electrolytic bath
- 32 , 34 electrode
- 36 power source for electrolysis
- 38 aqueous solution of an organic electrolyte.
- an aqueous solution of an organic electrolyte comprising substantially exclusively the organic electrolyte is electrolyzed to give a mixed electrolyzed water consisting of the resulting anodic and cathodic electrolyzed waters.
- the mixed electrolyzed water has an increased dissociation ratio of the organic electrolyte and improved ability to dismutate superoxide radical, and can be thus used in a variety of applications such as skin care.
- FIG. 1 schematically shows a configuration of an electrolysis apparatus used in a process for preparing a mixed electrolyzed water of this invention.
- 2 denotes a raw electrolysis water reservoir, which is filled with an aqueous solution of an organic electrolyte (raw electrolysis water) 4 .
- the aqueous solution of an organic electrolyte 4 contains 1 to 50 mM, preferably 2 to 20 mM of the organic electrolyte.
- a concentration of the organic electrolyte is less than 1 mM, an electric conductivity is too low to conduct electrolysis. If a concentration of the organic electrolyte is more than 50 mM, the mixed electrolyzed water obtained feels sticky, for example, when being applied to the skin for care, and may be inconvenient for some applications.
- Examples of an organic electrolyte include ascorbic acid and its water-soluble derivatives, water-soluble flavonoids, water-soluble polyphenols including water-soluble catechins, and water-soluble extract from herbs.
- the aqueous solution of the organic electrolyte 4 substantially contains, besides the organic electrolyte, no electrolytes such as water-soluble inorganic salts.
- a content of the water-soluble inorganic electrolytes is preferably up to 0.1 mM, particularly preferably up to 0.02 mM as the total of these water-soluble inorganic electrolytes.
- a concentration of the inorganic electrolytes is much lower than that of inorganic electrolytes in common well water, tap water or the like.
- Such an aqueous solution of an organic electrolyte 4 can be prepared, for example, by dissolving an organic electrolyte such as ascorbic acid in purified water (pure water) such as distilled water and deionized water within the above concentration range.
- purified water purified water
- 6 is a pump intervening a feeding line 8 through which an aqueous solution of an organic electrolyte is fed.
- the aqueous solution of an organic electrolyte 4 is fe d to a non-diaphragm electrolytic bath 10 via the feeding line 8 .
- a distance between the paired electrodes 12 , 14 is no more than 2 mm, preferably 1.5 to 0.05 mm, more preferably 1.0 to 0.1 mm. If the inter-electrode distance is more than 2 mm, an anodic and a cathodic electrolyzed waters generated by electrolysis cannot be adequately mixed, leading to inadequate increase in a dissociation ratio of hydroxyl group of the organic electrolyte in the resulting mixed electrolyzed water or inadequate dismutation of superoxide radical.
- a diaphragm between the electrodes 12 , 14 in the electrolytic bath 10 is undesirable because it causes inadequate mixing of the anodic and the cathodic electrolyzed waters generated by electrolysis.
- the electrodes 12 , 14 are made of an electrochemically inactive metal material.
- Preferable electrode materials include platinum and platinum alloys. Although a pair of electrodes has been used in the above description, multiple pairs of electrodes may be, without limitations, placed in an electrolytic bath for improving an electrolysis efficiency.
- 16 is a power source for electrolysis, whose plus and minus terminals are connected to the above electrodes 12 and 14 via interconnections 18 and 20 , respectively.
- Polarity of an electric power applied to each of the electrodes may be constant or alternated at an appropriate time interval. Alternation of polarity of an applied power at a certain time interval is particularly preferable because a cathodic and an anodic electrolyzed waters are alternately generated in one electrode, resulting in effective mixing of the anodic and the cathodic electrolyzed waters.
- An interval of polarity alternation is preferably 2 to 60 times/min.
- the aqueous solution of an organic electrolyte 4 passes through the feeding line for an aqueous solution of an organic electrolyte 8 and is fed into the non-diaphragm electrolytic bath 10 , in which the solution is then subjected to electrolysis.
- An electrolysis current density is preferably 0.003 to 0.03 A/cm 2 , particularly preferably 0.01 to 0.02 A/cm 2 .
- An electrolysis current density of less than 0.003 A/cm 2 may lead to inadequate increase in a dissociation ratio of hydroxyl group in the organic electrolyte in the resulting mixed electrolyzed water or inadequate dismutation for superoxide radical.
- an electrolysis current density of more than 0.03 A/cm 2 may be uneconomical because a dissociation ratio of hydroxyl group in the organic electrolyte in the mixed electrolyzed water is not increased or dismutation for superoxide radical is not increased in proportion to the higher current value.
- an electrolysis current density can be controlled within the above range to obtain an at least 1.25-fold dissociation ratio of hydroxyl group in the mixed electrolyzed water discharged from the electrolytic bath in comparison with that before electrolysis and an at least 1.2-fold dismutation for superoxide radical in comparison with that before electrolysis.
- Electrolysis as described above results in spontaneous mixing of an anodic and cathodic electrolyzed waters generated in an electrolytic bath during electrolysis.
- the mixed electrolyzed water as a mixture of both electrolyzed waters is continuously drained through a drain line for a mixed electrolyzed water 22 .
- Presence of ability to dismutating superoxide radical may be determined by observing a superoxide radical signal in ESR described later.
- FIG. 2 shows another embodiment of an electrolysis apparatus.
- an aqueous solution of an organic electrolyte is not continuously fed to an electrolytic bath.
- 30 is a non-diaphragm electrolytic bath, in which there are placed a pair of mutually facing electrodes 32 , 34 in parallel.
- 36 is a power source for electrolysis, which supplies electrolysis power to the electrodes 32 , 34 .
- the electrolytic bath is filled with an aqueous solution of an organic electrolyte 38 . Electric power is supplied from the power source for electrolysis 36 to these electrodes 32 , 34 , to initiate electrolysis of the aqueous solution of an organic electrolyte 38 . Details of the configuration is as described above, and thus are not described herein.
- a raw electrolysis water containing ascorbic acid (AsA) was electrolyzed using an electrolysis apparatus shown in FIG. 2 .
- Electrolysis was conducted while alternating polarity at an interval of 30 sec after the initiation of electrolysis.
- Table 1 shows pH, a oxidation-reduction potential (ORP), a dissolved oxygen content (DO) and an electrical conductivity (EC) of the mixed electrolyzed water generated by electrolysis.
- ORP oxidation-reduction potential
- DO dissolved oxygen content
- EC electrical conductivity
- Measurement temperature an ambient temperature
- Microwave output 3.7 mW
- Modulation width 0.1 mT
- an ORP was reduced, i.e., it moved toward a reducing side. Furthermore, a DO was reduced, indicating that an anodic and a cathodic electrolyzed waters were effectively mixed, resulting in a reduced DO. In addition, an EC did not vary very much, indicating that the number of electrolyte ions little varied.
- FIG. 3 demonstrates that along with elapse of an electrolysis time, a titration volume was reduced, indicating that a dissociation ratio of the mixed electrolyzed water generated by electrolysis was increased. Furthermore, table 1 shows that pH of the mixed electrolyzed water was little varied.
- FIG. 4 indicates that along with elapse of an electrolysis time, ability to dismutate superoxide radical was increased.
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Abstract
This invention discloses a process for preparing a mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters comprising the step of electrolyzing an aqueous solution of an organic electrolyte containing a water-soluble inorganic salt in less than 0.1 mM and an organic electrolyte in 1 to 50 mM which is fed into a non-diaphragm electrolytic bath comprising at least a pair of inactive electrodes separated from each other by an inter-electrode distance of 2 mm or less, wherein the aqueous solution of an organic electrolyte with pH equal to that of the mixed electrolyzed water prepared by electrolysis is neutralized with a titration volume less than that for the raw aqueous solution in neutralization titration with an aqueous solution of sodium hydroxide or has a higher dismutation activity to superoxide radical per mole than the raw aqueous solution.
Description
- This invention relates to a process for preparing a mixed electrolyzed water consisting of an anodic and a cathodic electrolyzed waters, which is capable of dismutating superoxide radical. In particular, this invention relates to a process for preparing a mixed electrolyzed water capable of dismutating superoxide radical, comprising electrolyzing an aqueous organic electrolyte solution containing virtually only an organic water-soluble electrolyte such a ascorbic acid as an electrolyte.
- It has been well-known that using an electrolytic bath within which inactive electrodes made of platinum or a platinum alloy are placed via a diaphragm, aqueous dilute electrolyte solution of an alkali-metal chloride is electrolyzed, followed by removing an anodic electrolyzed water (acidic water) with a lower pH generated in the anodic side, which can be utilized for sterilization or disinfection. Examples of a diaphragm used include a charged membrane which is an ion-exchange resin film and a non-charged membrane having a microporous structure.
- In an anodic electrolyzed water, hypochlorous acid is generated. Thus, because of strong oxidizing and chlorinating action of hypochlorous acid, an anodic electrolyzed water is used for sterilization or disinfection. Such utilization is prevalent in medical institutions and so on. Further, since small amounts of ozone and dissolved oxygen present in the acidic water promotes granulation, it has been studied as an aid for surgical treatment.
- Meanwhile, a cathodic electrolyzed water (alkaline water) in the cathodic side can be generated by electrolyzing tap water in place of a dilute electrolytic solution, and has been used for drinking. It has been also reported that to the aqueous electrolytic solution, an organic acid such as ascorbic acid and gallic acid is added, not as an electrolytic aid, but as an additive.
- In this technique, ascorbic acid is used in the presence of an electrolytic aid. Ascorbic acid is added for controlling pH of a cathodic electrolyzed water and for removing free chlorine in an anodic electrolyzed water.
- Ascorbic acid has OH groups at 2- and 3-positions. In an acidic range, the OH at 3-position is dissociated into —O− and H+, having acidity. In an alkaline range, although the OH at 2-position is dissociated into —O− and H+, its dissociation ratio is too low to allow ascorbic acid to be used as an electrolytic aid. An electrolysis mechanism for an aqueous solution of ascorbic acid is so complicated that intermediates have not been identified, but the mechanism is observed a redox reaction in principle.
- Ascorbic acid is a strong reducing agent by itself. It is, however, well-known that ascorbic acid undergoes autoxidation in an aqueous solution, leading to deteriorated reducing power.
-
- wherein AsA, MDA, DHA and DKG represent ascorbic acid, monodehydroascorbic acid, dehydroascorbic acid and 2,3-diketogulonic acid, respectively.
- It has been recently demonstrated that ascorbic acid can dismutate and eliminate superoxide radical which is generally known as “active oxygen”. Therefore, ascorbic acid has drawn attention as an antioxidant.
- Dismutation is a reaction where superoxide radical is decomposed and hydrogen peroxide generates as shown in equation (2):
O− 2·+O− 2·+2H+→H2O2+O2 (2) - As described above, ascorbic acid is a useful compound for eliminating superoxide radical.
- We have focused on dismutating effect of ascorbic acid on superoxide radical and have intensely studied to obtain an anodic electrolyzed water having the above ability. We have consequently found that an anodic electrolyzed water having ability of dismutating superoxide radical can be obtained by electrolyzing an aqueous solution containing only ascorbic acid in a relatively low concentration without using any inorganic electrolyte such as a water-soluble metal salt as an electrolytic aid. We have thus filed a patent application on a process for preparing an anodic electrolyzed water having improved ability to dismutate superoxide radical (Japanese Patent Application No. 20010-172538, claim 1).
- In the above process for preparing an anodic electrolyzed water having improved ability to dismutate superoxide radical, a cathodic electrolyzed water is discarded without being effectively utilized because it has been believed that OH—ion present in a cathodic electrolyzed water would react with dissociated ascorbate ion (AsA−) to oxidize ascorbic acid, as shown in equation (3):
AsA−+OH−→AsAO+H2 (3) - We have intensely attempted to solve the above problem, and have finally found that when mixing an anodic electrolyzed water with a cathodic electrolyzed water, oxygen generated in an anode side and hydrogen generated in a cathode side quickly react to form water so that oxidation of ascorbic acid actually fails to occur. Furthermore, it has been found that these electrolyzed waters can be effectively mixed by selecting a inter-electrode distance not more than a certain value; that thus a mixed electrolyzed water having improved ability of dismutating superoxide radical can be effectively prepared; and further that there is no need to discharge a cathodic electrolyzed water as waste.
- Based on the above findings, this invention has been achieved. An objective of this invention is to provide a process for preparing a mixed electrolyzed water having improved ability to dismutate superoxide radical.
- Since a raw electrolysis water comprising an organic electrolyte as an electrolysis aid is electrolyzed and resulting an anodic and a cathodic electrolyzed waters are mixed in this invention, the mixed electrolyzed water thus obtained has a lower DO and improved ability to dismutate superoxide radical. The mixed electrolyzed water can be, therefore, conveniently used in a variety of applications such as sterilization, disinfection, granulation, health maintenance and cosmetic applications. Furthermore, since an anodic and a cathodic electrolyzed waters are prepared as a mixture in this preparation process, it requires a simpler manufacturing apparatus in comparison with a conventional process where an electrolyzed water in one electrode side is taken out. Additionally, since as an organic electrolyte, this invention employs a vitamin, ascorbic acid whose safety to a human body has been established, the cathodic electrolyzed water prepared using the vitamin as an electrolysis aid is also quite safe.
- This invention will be described below.
- [1] A process for preparing a mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters comprising the step of electrolyzing an aqueous solution of an organic electrolyte containing a water-soluble inorganic salt in less than 0.1 mM and an organic electrolyte in 1 to 50 mM which is fed into a non-diaphragm electrolytic bath comprising at least a pair of inactive electrodes separated from each other by an inter-electrode distance of 2 mm or less, wherein the aqueous solution of an organic electrolyte with pH equal to that of the mixed electrolyzed water prepared by electrolysis is neutralized with a titration volume less than that for the raw aqueous solution in neutralization titration with an aqueous solution of sodium hydroxide or has a higher dismutating activity to superoxide radical per mole than the raw aqueous solution.
- [2] The process for preparing a mixed electrolyzed water as described in [1], wherein a polarity of electric power supplied between both electrodes at least one irreversible electrodes is alternated at least twice per minute.
- [3] The process for preparing a mixed electrolyzed water as described in [1], wherein the organic electrolyte is selected from the group consisting of ascorbic acid and its water-soluble derivatives, water-soluble flavonoids, water-soluble polyphenols including water-soluble catechins and a water-soluble extract from herbs.
- Generally, on being dissolved in water, an inorganic electrolyte such as sodium chloride, potassium chloride, calcium chloride and sodium sulfate is ionized, i.e., completely dissociated into a cation and an anion. Specific properties of water as a solvent is responsible to dissociation of these salts. Water dissolves and ionizes an electrolyte, i.e., a substance comprised of electrically positive and negative elements, and loosens association between molecules comprised of a positive and a negative elements. Once a solid electrolyte with limited freedom is dissolved in water, its freedom is so increased that the electrolyte can be attached to or detached from a variety of chemical species.
- Some of electrolytes inherently has lower freedom. Such an electrolyte may not be, although being dissolved to some degree, completely dissolved in water and some part of the electrolyte remains insoluble. Examples of a measure of freedom in an aqueous solution include a dissociation constant, a dissociation index and an electrolytic dissociation constant.
- A dissociation constant is defined as below. For example, when an electrolyte AB is dissociated or ionized into A+ and B− in water as shown in equation (3), a relationship shown in equation (4) holds true:
ABA + +B − (3)
[A + ] [B − ]/[AB]=Ka (4) - wherein [A+], [B−] and [AB] represent concentrations of the corresponding entities.
- A dissociation ratio herein is called a dissociation constant, Ka, provided that in terms of conditions, a temperature and a pressure must be constant; specifically 25° C. and 1 atm, respectively, in a standard condition.
- Thus, a dissociation constant of a water-soluble electrolyte indicates a degree of its dissociation.
-
- This reaction proceeds in a strong electric-field layer in the electrode surface, so that the reaction is initiated by an extremely minute electric energy.
- Furthermore, in electrolysis of a dilute aqueous electrolytic solution, water itself is electrolyzed while its dissociation is accelerated.
-
- As indicated in this equation, only 10−7 mols are ionized in one liter neutral water.
- However, electrolysis can accelerate ionization to increase the numbers of positive and negative ions (See, J. Appl. Electrochem. 31 (2001), 1307-1313).
- When applying an electric field such that a direct current flows through an aqueous solution of an organic electrolyte with a relatively lower ionization degree, substances having positive charge and having negative charge move toward a cathode and an anode, respectively. Then, they are involved in giving and receiving electrons in the surfaces, to give corresponding electrode-reaction products.
- Simultaneously, water itself is oxidized in the anode while being reduced in the cathode, and the resulting products react with the ionized organic electrolyte to give new products. In particular, in the course of oxidation and reduction of water, dissociation of water proceeds, resulting in a minor increase in a water ion product. Furthermore, the ionized organic electrolyte is also dissociated to a minor degree to reduce a dissociation index.
- Relative dissociation rates may be compared by neutralization titration of the raw aqueous solution before electrolysis and the resulting solution after electrolysis with an acid or base, followed by determination of a difference between the titration volumes. A smaller titration volume indicates a larger dissociation in an aqueous solution.
- The above principle will be more specifically described using L-ascorbic acid (AsA) as an organic electrolyte.
-
- In AsA, reactive sites are essentially OH groups attached at 2- and 3-positions, which are dissociated into H+ and —O−. For example, dissociation for the OH group at 3-position is expressed as pKa=4.25. A dissociation index of 4.25 indicates that the OH group is dissociated into a positive and a negative ions in an extremely small amount of (½)×10−4.25 M (2.8117×10−5 M) in an aqueous solution up to one liter at 1 atm and 25° C.
- When dissolving a low-dissociating substance such as AsA in a cathodic electrolyzed water prepared by electrolysis of a dilute aqueous electrolyte solution such as a brine, the dissociation is accelerated. As a result, it has been found that a dismutation activity (SOD activity) for superoxide radical is increased in comparison with an aqueous solution of AsA with an equal pH. Specifically, dissociation of the OH group at 2-position of AsA shown in formula (8) is accelerated so that the group becomes more reactive to a radical or active oxygen. The OH group at 3-position is consumed in neutralization of the alkaline electrolyzed water. The OH, therefore, exhibits lower SOD activity and does not act as a scavenger to superoxide radical. Thus, a low-dissociating water-soluble substance can be dissolved in an electrolyzed water with improved dissociating ability, to improve dissociation of the substance, resulting in making the low-dissociating water-soluble substance more reactive.
- As shown in formula (8), ascorbic acid has dissociable groups (—OH group) at 2- and 3-positions, which have dissociation indices, pKa, of 11.79 and 4.25, respectively, at 25° C. and 1 atm. In an aqueous solution, the OH group at 3-position in ascorbic acid is dissociated into —O− and H+, exhibiting acidity. A mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters prepared by electrolysis of a dilute aqueous solution of ascorbic acid contains no electrolyte ions other than ascorbate ions. The OH group at 3-position is, therefore, not used in neutralization of an alkali generated in the cathode, maintaining its reducibility.
- When an anode and a cathode are closely disposed to each other and these electrodes are not separated by a diaphragm, an anodic and a cathodic electrolyzed waters may be easily mixed. As a result, dissolved oxygen generated by anodic oxidation of water reacts with dissolved hydrogen generated by cathodic reduction of water, to give water. In addition, polarity of an electrolysis voltage can be alternated to further accelerate mixing of the anodic and the cathodic electrolyzed waters. Consequently, a concentration of dissolved oxygen generated by oxidation of water in the anode becomes 1 mg/L or less because the dissolved oxygen is consumed by AsA and reducing dissolved hydrogen.
- As described above, a dissociation ratio can be compared by a neutralization volume for a substance such as AsA which exhibits acidity by a 2,3-ene diol, while the dissociation ratio can be compared by determining dismutating ability for superoxide radical to a substance which does not exhibit acidity or basicity.
- Generally, water-soluble polyphenols, water-soluble flavonoids and catechins exhibit dismutating ability to superoxide radical. As in AsA, proton from an OH group attached to a carbon atom having a double bond exhibits dismutating ability. For example, in quercetine, a flavonoid, a proton from the OH group exhibits antioxidation ability as shown in equation (9).
O2 −·+2H+→H2O2 (9) - These water-soluble antioxidants release free proton to dismutate superoxide radical. As described above, proton dissociation is accelerated by electrolysis, resulting in increased dismutating ability per mol. It is, therefore, possible to estimate a dissolution degree by comparing dismutating ability.
-
-
FIG. 1 schematically shows a configuration of an apparatus for preparing a mixed electrolyzed water used in a process for preparing a mixed electrolyzed water of this invention. -
FIG. 2 schematically shows another configuration of an apparatus for preparing a mixed electrolyzed water used in a process for preparing a mixed electrolyzed water of this invention. -
FIG. 3 is a graph showing relationship between an electrolysis time for a mixed electrolyzed water in Example 1 and a neutralization volume of a 0.1 N aqueous solution of NaOH. -
FIG. 4 is a graph showing a relationship between an electrolysis time for a mixed electrolyzed water prepared by electrolyzing an aqueous solution of an organic electrolyte and a dismutating ability to superoxide radical. - In these drawings, the symbols have the following meanings; 2: raw electrolysis water reservoir; 4: aqueous solution of an organic electrolyte; 6: pump; 8: feeding line for an aqueous solution of an organic electrolyte; 10: non-diaphragm electrolytic bath; 12, 14: electrode; 16: power source for electrolysis; 18, 20: interconnections; 22: drain line for a mixed electrolyzed water; 30: non-diaphragm electrolytic bath; 32, 34: electrode; 36: power source for electrolysis; 38: aqueous solution of an organic electrolyte.
- In this invention, an aqueous solution of an organic electrolyte comprising substantially exclusively the organic electrolyte is electrolyzed to give a mixed electrolyzed water consisting of the resulting anodic and cathodic electrolyzed waters. The mixed electrolyzed water has an increased dissociation ratio of the organic electrolyte and improved ability to dismutate superoxide radical, and can be thus used in a variety of applications such as skin care.
-
FIG. 1 schematically shows a configuration of an electrolysis apparatus used in a process for preparing a mixed electrolyzed water of this invention. In this figure, 2 denotes a raw electrolysis water reservoir, which is filled with an aqueous solution of an organic electrolyte (raw electrolysis water) 4. - The aqueous solution of an
organic electrolyte 4 contains 1 to 50 mM, preferably 2 to 20 mM of the organic electrolyte. When a concentration of the organic electrolyte is less than 1 mM, an electric conductivity is too low to conduct electrolysis. If a concentration of the organic electrolyte is more than 50 mM, the mixed electrolyzed water obtained feels sticky, for example, when being applied to the skin for care, and may be inconvenient for some applications. - Examples of an organic electrolyte include ascorbic acid and its water-soluble derivatives, water-soluble flavonoids, water-soluble polyphenols including water-soluble catechins, and water-soluble extract from herbs.
- The aqueous solution of the
organic electrolyte 4 substantially contains, besides the organic electrolyte, no electrolytes such as water-soluble inorganic salts. A content of the water-soluble inorganic electrolytes is preferably up to 0.1 mM, particularly preferably up to 0.02 mM as the total of these water-soluble inorganic electrolytes. A concentration of the inorganic electrolytes is much lower than that of inorganic electrolytes in common well water, tap water or the like. - Such an aqueous solution of an
organic electrolyte 4 can be prepared, for example, by dissolving an organic electrolyte such as ascorbic acid in purified water (pure water) such as distilled water and deionized water within the above concentration range. - In this figure, 6 is a pump intervening a
feeding line 8 through which an aqueous solution of an organic electrolyte is fed. By actuating thispump 6, the aqueous solution of anorganic electrolyte 4 is fe d to a non-diaphragmelectrolytic bath 10 via thefeeding line 8. - Inside of the non-diaphragm
electrolytic bath 10, there are placed a pair of mutually facingelectrodes electrodes - Furthermore, a diaphragm between the
electrodes electrolytic bath 10 is undesirable because it causes inadequate mixing of the anodic and the cathodic electrolyzed waters generated by electrolysis. - The
electrodes - In the figure, 16 is a power source for electrolysis, whose plus and minus terminals are connected to the
above electrodes interconnections - The aqueous solution of an
organic electrolyte 4 passes through the feeding line for an aqueous solution of anorganic electrolyte 8 and is fed into the non-diaphragmelectrolytic bath 10, in which the solution is then subjected to electrolysis. An electrolysis current density is preferably 0.003 to 0.03 A/cm2, particularly preferably 0.01 to 0.02 A/cm2. An electrolysis current density of less than 0.003 A/cm2 may lead to inadequate increase in a dissociation ratio of hydroxyl group in the organic electrolyte in the resulting mixed electrolyzed water or inadequate dismutation for superoxide radical. On the other hand, an electrolysis current density of more than 0.03 A/cm2 may be uneconomical because a dissociation ratio of hydroxyl group in the organic electrolyte in the mixed electrolyzed water is not increased or dismutation for superoxide radical is not increased in proportion to the higher current value. - Therefore, an electrolysis current density can be controlled within the above range to obtain an at least 1.25-fold dissociation ratio of hydroxyl group in the mixed electrolyzed water discharged from the electrolytic bath in comparison with that before electrolysis and an at least 1.2-fold dismutation for superoxide radical in comparison with that before electrolysis.
- Electrolysis as described above results in spontaneous mixing of an anodic and cathodic electrolyzed waters generated in an electrolytic bath during electrolysis. The mixed electrolyzed water as a mixture of both electrolyzed waters is continuously drained through a drain line for a mixed
electrolyzed water 22. - Presence of ability to dismutating superoxide radical may be determined by observing a superoxide radical signal in ESR described later.
-
FIG. 2 shows another embodiment of an electrolysis apparatus. In this embodiment, an aqueous solution of an organic electrolyte is not continuously fed to an electrolytic bath. InFIG. 2, 30 is a non-diaphragm electrolytic bath, in which there are placed a pair of mutually facingelectrodes electrodes organic electrolyte 38. Electric power is supplied from the power source forelectrolysis 36 to theseelectrodes organic electrolyte 38. Details of the configuration is as described above, and thus are not described herein. - This invention will be more specifically described with reference to examples.
- A raw electrolysis water containing ascorbic acid (AsA) was electrolyzed using an electrolysis apparatus shown in
FIG. 2 . - In an electrolytic bath was placed a pair of electrodes which are a 10×10 cm titanium plate coated with platinum. An inter-electrode distance was 2 mm. The electrolytic bath was a rectangular solid with a size of 11 cm (length)×5 cm (width)×12 cm (height). A 30 mM aqueous solution of AsA was prepared and 600 mL of the solution was charged in the electrolytic bath. While stirring the aqueous AsA solution, a current of 0.25 A was applied to the electrodes to conduct electrolysis. Electrolysis was conducted while alternating polarity at an interval of 30 sec after the initiation of electrolysis. Table 1 shows pH, a oxidation-reduction potential (ORP), a dissolved oxygen content (DO) and an electrical conductivity (EC) of the mixed electrolyzed water generated by electrolysis. Mixed electrolyzed water generated at various electrolysis times were netralization-titrated with a 0.1 N NaOH solution and the titration amounts were compared. The results are shown in
FIG. 3 . Furthermore,FIG. 4 shows the results of superoxide-radical dismutating ability of AsA, a water-soluble flavonoid and a water-soluble catechin, which were measured by an electron spin resonance spectroscope. The conditions in signal measurement using the electron spin resonance spectroscope are as follows: - Measurement temperature: an ambient temperature;
- Microwave output: 3.7 mW;
- Sweeping field: 339.1 mT+5.5 mT;
- Field modulation: 100 kHz (external modulation mode);
- Modulation width: 0.1 mT;
- Time constant: 0.12 sec;
- Sweeping time: 1 min.
TABLE 1 Electrolysis time (sec) 0 30 90 PH 3.01 3.02 3.02 ORP (mV) 148 109 80 DO (mg/L) 6.62 0.5 0.01 EC (mS/m) 46.5 46.4 46.3 - As seen from Table 1, along with elapse of an electrolysis time, an ORP was reduced, i.e., it moved toward a reducing side. Furthermore, a DO was reduced, indicating that an anodic and a cathodic electrolyzed waters were effectively mixed, resulting in a reduced DO. In addition, an EC did not vary very much, indicating that the number of electrolyte ions little varied.
-
FIG. 3 demonstrates that along with elapse of an electrolysis time, a titration volume was reduced, indicating that a dissociation ratio of the mixed electrolyzed water generated by electrolysis was increased. Furthermore, table 1 shows that pH of the mixed electrolyzed water was little varied. -
FIG. 4 indicates that along with elapse of an electrolysis time, ability to dismutate superoxide radical was increased.
Claims (3)
1. A process for preparing a mixed electrolyzed water consisting of a cathodic and an anodic electrolyzed waters comprising the step of electrolyzing an aqueous solution of an organic electrolyte containing a water-soluble inorganic salt in less than 0.1 mM and an organic electrolyte in 1 to 50 mM which is fed into a non-diaphragm electrolytic bath comprising at least a pair of inactive electrodes separated from each other by an inter-electrode distance of 2 mm or less, wherein the aqueous solution of an organic electrolyte with pH equal to that of the mixed electrolyzed water prepared by electrolysis is neutralized with a titration volume less than that for the raw aqueous solution in neutralization titration with an aqueous solution of sodium hydroxide or has a higher dismutation activity to superoxide radical per mole than the raw aqueous solution.
2. The process for preparing a mixed electrolyzed water as claimed in claim 1 , wherein a polarity of electric power supplied between both electrodes at least one irreversible electrodes is alternated at least twice per minute.
3. The process for preparing a mixed electrolyzed water as claimed in claim 1 , wherein the organic electrolyte is selected from the group consisting of ascorbic acid and its water-soluble derivatives, water-soluble flavonoids, water-soluble polyphenols including water-soluble catechins and a water-soluble extract from herbs.
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JP2003046903A JP2004261656A (en) | 2003-02-25 | 2003-02-25 | Manufacturing method for mixed electrolytic water |
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PCT/JP2004/002083 WO2004076363A1 (en) | 2003-02-25 | 2004-02-23 | Method for producing mixed electrolyzed water |
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US10/545,341 Abandoned US20060065544A1 (en) | 2003-02-25 | 2004-02-23 | Process for preducing mixed electrolyzed water |
Country Status (7)
Country | Link |
---|---|
US (1) | US20060065544A1 (en) |
EP (1) | EP1598317A4 (en) |
JP (1) | JP2004261656A (en) |
KR (1) | KR20050107446A (en) |
CN (1) | CN100343179C (en) |
TW (1) | TW200424133A (en) |
WO (1) | WO2004076363A1 (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080292716A1 (en) * | 2006-04-28 | 2008-11-27 | Kokichi Hanaoka | Electrolytic Water and Process for Production Thereof |
US20080317664A1 (en) * | 2005-12-21 | 2008-12-25 | Shanghai Institute Of Applied Physics, Chinese Academy Of Sciences | Method for Gas Storage |
US20100276278A1 (en) * | 2009-04-30 | 2010-11-04 | Doug Bender | Modular electrolysis device |
WO2013162733A1 (en) * | 2012-04-23 | 2013-10-31 | Nbip, Llc | Dermal-appropriate compositions and methods of use |
WO2015042475A1 (en) * | 2013-09-19 | 2015-03-26 | Reoxcyn Discoveries Group, Inc. | Life enhancing beverages |
US9089602B2 (en) | 2008-07-25 | 2015-07-28 | Reven Pharmaceuticals, Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
US9572810B2 (en) | 2010-07-22 | 2017-02-21 | Reven Pharmaceuticals, Inc. | Methods of treating or ameliorating skin conditions with a magnetic dipole stabilized solution |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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ITMI20071863A1 (en) * | 2007-09-28 | 2009-03-29 | Industrie De Nora Spa | ELECTROCHEMICAL DEVICE FOR BIOCIDAL TREATMENT IN AGRICULTURAL APPLICATIONS |
CN101767848B (en) * | 2008-12-30 | 2012-07-18 | 肖志邦 | Functional water preparation method and device by undivided micro-current electrolysis |
JP2014200778A (en) * | 2013-04-10 | 2014-10-27 | 至明 松尾 | Antioxidative drinking water |
CN105217738A (en) * | 2014-06-24 | 2016-01-06 | 罗民雄 | A kind of method and apparatus having one or more water entrys also water inlet can be made brine electrolysis |
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US4895678A (en) * | 1987-09-16 | 1990-01-23 | Doryokuro Kakunenryo Kaihatsu Jigyodan | Method for thermal decomposition treatment of radioactive waste |
US6921743B2 (en) * | 2001-04-02 | 2005-07-26 | The Procter & Gamble Company | Automatic dishwashing compositions containing a halogen dioxide salt and methods for use with electrochemical cells and/or electrolytic devices |
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JPH1160429A (en) * | 1997-08-26 | 1999-03-02 | Matsushita Electric Works Ltd | Preparation of cleansing lotion, preparation apparatus for cleansing lotion and cleansing lotion |
JP3602773B2 (en) * | 2000-06-08 | 2004-12-15 | 株式会社ミクニ | Anode electrolyzed water and method for producing the same |
JP2002301476A (en) * | 2001-04-10 | 2002-10-15 | Mikuni Corp | Ascorbylglucosamine electrolyzed water and method for making the same |
-
2003
- 2003-02-25 JP JP2003046903A patent/JP2004261656A/en active Pending
-
2004
- 2004-02-20 TW TW093104321A patent/TW200424133A/en unknown
- 2004-02-23 KR KR1020057015543A patent/KR20050107446A/en not_active Application Discontinuation
- 2004-02-23 US US10/545,341 patent/US20060065544A1/en not_active Abandoned
- 2004-02-23 EP EP04713663A patent/EP1598317A4/en not_active Withdrawn
- 2004-02-23 WO PCT/JP2004/002083 patent/WO2004076363A1/en active Application Filing
- 2004-02-23 CN CNB2004800051243A patent/CN100343179C/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US4895678A (en) * | 1987-09-16 | 1990-01-23 | Doryokuro Kakunenryo Kaihatsu Jigyodan | Method for thermal decomposition treatment of radioactive waste |
US6921743B2 (en) * | 2001-04-02 | 2005-07-26 | The Procter & Gamble Company | Automatic dishwashing compositions containing a halogen dioxide salt and methods for use with electrochemical cells and/or electrolytic devices |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080317664A1 (en) * | 2005-12-21 | 2008-12-25 | Shanghai Institute Of Applied Physics, Chinese Academy Of Sciences | Method for Gas Storage |
US20080292716A1 (en) * | 2006-04-28 | 2008-11-27 | Kokichi Hanaoka | Electrolytic Water and Process for Production Thereof |
US11110053B2 (en) | 2008-07-25 | 2021-09-07 | Reven Pharmaceuticals Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
US9775798B2 (en) | 2008-07-25 | 2017-10-03 | Reven Pharmaceuticals, Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
US9089602B2 (en) | 2008-07-25 | 2015-07-28 | Reven Pharmaceuticals, Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
US9089511B2 (en) | 2008-07-25 | 2015-07-28 | Reven Pharmaceuticals, Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
US9101537B2 (en) | 2008-07-25 | 2015-08-11 | Reven Pharmaceuticals, Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
US20100276278A1 (en) * | 2009-04-30 | 2010-11-04 | Doug Bender | Modular electrolysis device |
US9572810B2 (en) | 2010-07-22 | 2017-02-21 | Reven Pharmaceuticals, Inc. | Methods of treating or ameliorating skin conditions with a magnetic dipole stabilized solution |
US9867849B2 (en) | 2010-07-22 | 2018-01-16 | Reven Pharmaceuticals, Inc. | Methods of treating or ameliorating skin conditions with a magnetic dipole stabilized solution |
US11202798B2 (en) | 2010-07-22 | 2021-12-21 | Reven Pharmaceuticals, Inc. | Method of treating or ameliorating skin conditions with a magnetic dipole stabilized solution |
AU2013252963B2 (en) * | 2012-04-23 | 2016-06-09 | Nbip, Llc | Dermal-appropriate compositions and methods of use |
WO2013162733A1 (en) * | 2012-04-23 | 2013-10-31 | Nbip, Llc | Dermal-appropriate compositions and methods of use |
WO2015042475A1 (en) * | 2013-09-19 | 2015-03-26 | Reoxcyn Discoveries Group, Inc. | Life enhancing beverages |
Also Published As
Publication number | Publication date |
---|---|
TW200424133A (en) | 2004-11-16 |
EP1598317A1 (en) | 2005-11-23 |
CN100343179C (en) | 2007-10-17 |
EP1598317A4 (en) | 2009-11-25 |
WO2004076363A1 (en) | 2004-09-10 |
JP2004261656A (en) | 2004-09-24 |
CN1753838A (en) | 2006-03-29 |
KR20050107446A (en) | 2005-11-11 |
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Legal Events
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AS | Assignment |
Owner name: KOKICHI HANAOKA, JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:HANAOKA, KOKICHI;REEL/FRAME:016811/0087 Effective date: 20050729 Owner name: MIKUNI CORPORATION, JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:HANAOKA, KOKICHI;REEL/FRAME:016811/0087 Effective date: 20050729 |
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STCB | Information on status: application discontinuation |
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