US20050159361A1 - Remedies for sex hormone-dependent disease - Google Patents

Remedies for sex hormone-dependent disease Download PDF

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US20050159361A1
US20050159361A1 US10/507,044 US50704404A US2005159361A1 US 20050159361 A1 US20050159361 A1 US 20050159361A1 US 50704404 A US50704404 A US 50704404A US 2005159361 A1 US2005159361 A1 US 2005159361A1
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receptor agonist
salt
antagonist
agent
androgen
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Takahito Hara
Masami Kusaka
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Takeda Pharmaceutical Co Ltd
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Takeda Pharmaceutical Co Ltd
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Assigned to TAKEDA PHARMACEUTICAL COMPANY LIMITED reassignment TAKEDA PHARMACEUTICAL COMPANY LIMITED CHANGE OF NAME (SEE DOCUMENT FOR DETAILS). Assignors: TAKEDA CHEMICAL INDUSTRIES, LTD.
Publication of US20050159361A1 publication Critical patent/US20050159361A1/en
Assigned to TAKEDA PHARMACEUTICAL COMPANY LIMITED reassignment TAKEDA PHARMACEUTICAL COMPANY LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: HARA, TAKAHITO, KUSAKA, MASAMI
Priority to US11/864,022 priority Critical patent/US8518890B2/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/08—Peptides having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones

Definitions

  • the present invention relates to a novel pharmaceutical agent comprising an LHRH (luteinizing hormone-releasing hormone) receptor agonist or antagonist or a salt thereof in combination with an androgen receptor agonist or a salt thereof, an agent for the prophylaxis or treatment of prostate cancer, which comprises a non-steroidal androgen receptor agonist or a salt thereof and a method for treating prostate cancer and the like.
  • LHRH leukinizing hormone-releasing hormone
  • Gonadotropin releasing hormone is a decapeptide consisting of 10 amino acids, which is produced in the hypothalamus and known to control secretion of luteinizing hormone (LH), follicle stimulating hormone (FSH) and the like through receptors considered to be present in the anterior pituitary, based on which GnRH shows a variety of physiological activities such as ovulation induction and the like. Therefore, specific and selective antagonists or agonists to these receptors control hormone action of GnRH produced from the hypothalamus, and control secretion of anterior pituitary hormone such as LH, FSH and the like. Therefore, secretion of estrogen in females and of testosterone in males is suppressed and a prophylactic or therapeutic effect on sex hormone-dependent diseases can be expected.
  • LH luteinizing hormone
  • FSH follicle stimulating hormone
  • leuprorelin acetate which is an agonist of gonadotropin releasing hormone receptor, has 20 to 100 times the activity of natural GnRH, and is a compound that is not easily metabolized. Repetitive administration thereof causes down regulation of GnRH receptors and decreases release and production of gonadotrophic hormone in the pituitary gland, which is demonstrated by, for example, reduced reactivity to gonadotrophic hormone and lowered productivity of testosterone to a sterilization level in the testes, and lowered productivity of estrogen in the ovaries.
  • hormone-dependent diseases such as prostate cancer, prostatic hypertrophy, masculinism, hypertrichiasis, male-pattern baldness, male infantile precocity, female infantile precocity, precocious puberty, breast cancer, uterine cancer, mastopathy, hysteromyoma, endometriosis and the like.
  • leuprorelin acetate has been used widely in clinical situations as a therapeutic drug for prostate cancer, breast cancer, endometriosis, hysteromyoma, precocious puberty and the like.
  • the present invention aims at provision of a pharmaceutical agent and a prophylactic or therapeutic method based on an LHRH agonist or antagonist or a salt thereof that improves a prophylactic or therapeutic effect on various hormone-dependent diseases.
  • the present inventors have conducted intensive studies in an attempt to solve the aforementioned problems and found that a combined use of an LHRH receptor agonist or antagonist or a salt thereof with an androgen receptor agonist or a salt thereof, which are exacerbating factors of hormone-dependent diseases, unexpectedly strikingly enhances a prophylactic or therapeutic effect on various diseases by an LHRH agonist or antagonist or a salt thereof, and reduces side effects. Based on these findings, the present inventors have further studied, which resulted in the completion of the present invention.
  • the present invention relates to
  • the present invention moreover relates to
  • FIG. 1 shows cell growth rates of LNCaP-FGC and LNCaP-hr (highly androgen sensitive cell lines) cultured in the presence of testosterone, wherein the transverse axis shows testosterone concentration and the vertical axis shows cell growth rates.
  • FIG. 2 shows cell growth rates of MDA PCa 2b and MDA PCa 2b-hr (highly androgen sensitive cell lines) cultured in the presence of testosterone, wherein the transverse axis shows testosterone concentration and the vertical axis shows cell growth rates.
  • the following are used as an LHRH receptor agonist or antagonist.
  • LHRH receptor agonist for example, peptides described in Treatment with LHRH analogs: Controversies and perspectives , The Parthenon Publishing Group Ltd. (1996), JP-A-3-503165, JP-A-3-101695, JP-A-7-97334 and JP-A-8-259460 and the like are used.
  • D type amino acid residue for R 3 for example, ⁇ -D-amino acid having up to 9 carbon atoms (e.g., D-Leu, Ile, Nle, Val, Nval, Abu, Phe, Phg, Ser, Thr, Met, Ala, Trp, ⁇ -Aibu) and the like are used.
  • substituent for R 3 for example, tert-butyl, tert-butoxy, tert-butoxycarbonyl, methyl, dimethyl, trimethyl, 2-naphthyl, indolyl-3-yl, 2-methylindolyl, benzyl-imidazo-2-yl and the like are used.
  • a C 1-4 alkyl group is preferable, which is exemplified by methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl and tert-butyl.
  • Peptide (I) is preferably any of the following formulas (a)-(j).
  • an amino acid corresponding to R 3 in the formula (I) is a D-form.
  • the peptide (I) or a salt thereof is particularly preferably leuprorelin or leuprorelin acetate.
  • leuprorelin acetate is an acetate of leuprorelin.
  • LHRH receptor antagonist for example, those disclosed in U.S. Pat. Nos. 4,086,219, 4,124,577, 4,253,997 and 4,317,815, or a peptide represented by the formula: wherein X is a hydrogen or tetrahydrofurylcarboxamide, Q is a hydrogen or methyl, A is nicotinoyl or N,N′-diethylamidino and B is isopropyl or N,N′-diethylamidino [hereinafter sometimes to be abbreviated as peptide (II)] or a salt thereof are used.
  • X is a hydrogen or tetrahydrofurylcarboxamide
  • Q is a hydrogen or methyl
  • A is nicotinoyl or N,N′-diethylamidino
  • B is isopropyl or N,N′-diethylamidino [hereinafter sometimes to be abbreviated as peptide (II)] or
  • X is preferably tetrahydrofurylcarboxamide, more preferably (2S)-tetrahydrofurylcarboxamide.
  • A is preferably nicotinoyl.
  • B is preferably isopropyl.
  • salt of peptide (II) a pharmacologically acceptable salt is preferably used.
  • salts with inorganic acids e.g., hydrochloric acid, sulfuric acid, nitric acid and the like
  • salts with organic acids e.g., carbonic acid, bicarbonic acid, succinic acid, acetic acid, propionic acid, trifluoroacetic acid and the like
  • the salt of peptide (II) is more preferably a salt with an organic acid (e.g., carbonic acid, bicarbonic acid, succinic acid, acetic acid, propionic acid, trifluoroacetic acid and the like).
  • the salt of peptide (II) is particularly preferably a salt with acetic acid. These salts may be a mono to tri salt.
  • the peptide (II) or a salt thereof preferably has the following formulas (1)-(4).
  • m is a real number of 1 to 3.
  • the aforementioned formulas (2) and (4) show salts or solvates.
  • the peptide (II) or a salt thereof is more preferably the aforementioned (1) or (2), which is particularly preferably an S-isomer.
  • the peptide (II) or a salt thereof can be produced by a known method, such as a method described in JP-A-3-101695 (EP-A 413209), Journal of Medicinal Chemistry , vol. 35, p. 3942 (1992) and the like, or a method analogous thereto.
  • linear peptide which is a derivative of LHRH (U.S. Pat. No. 5,140,009, U.S. Pat. No. 5,171,835), a cyclic hexapeptide derivative (JP-A-61-191698), a bicyclic peptide derivative ( Journal of Medicinal Chemistry , vol. 36, pp. 3265-3273 (1993)) and the like.
  • non-peptide compound having an LHRH antagonistic action compounds described in JP-A-62-116514, WO 95/28405 (JP-A-8-295693), WO 97/14697 (JP-A-9-169767), WO 97/14682 (JP-A-9-169735), WO 96/24597 (JP-A-9-169768) and the like, J. Med. Chem ., vol. 32, pp. 2036-2038 (1989), and the like, and the like can be used.
  • LHRH receptor antagonist abarelix, ganirelix, cetrorelix, 5-(N-benzyl-N-methylaminomethyl)-1-(2,6-difluorobenzyl)-6-[4-(3-methoxyureido)phenyl]-3-phenylthieno[2,3-d]pyrimidine-2,4(1H,3H)-dione, 5-(N-benzyl-N-methylaminomethyl)-1-(2,6-difluorobenzyl)-6-[4-(3-ethylureido)phenyl]-3-phenylthieno[2,3-d]pyrimidine-2,4(1H,3H)-dione, 5-(N-benzyl-N-methylaminomethyl)-1-(2,6-difluorobenzyl)-6-[4-(3-ethylureido)phenyl]-3-phenylthieno[2,3-d]
  • the androgen receptor agonist a steroidal androgen receptor agonist and a non-steroidal androgen receptor agonist can be used.
  • endogenous androgens such as dehydroepiandrosterone, testosterone, dihydrotestosterone (DHT), androstenedione and the like
  • synthetic androgens such as Mestanolone, Oxymesterone, Methandrostenolone, Fluoxymesterone, Chlorotestosterone acetate, Methenolone acetate, Oxymetholone, Stanozolol, Furazabol, Oxandrolone, 19-Nortestosterone, Norethandrolone, Ethylestrenol, Norbolethone and the like, and the like can be used.
  • LGD-2226 As the non-steroidal androgen receptor agonist, LGD-2226 and the like can be used.
  • the aforementioned compounds alone or 2 or more compounds in combination can be used, with preference given to testosterone, dihydrotestosterone and the like.
  • estrogen receptor agonist steroidal estrogen receptor agonists such as estradiol, estrone, estriol and the like; and synthetic estrogen (non-steroidal estrogen) receptor agonists such as stilbestrol, diethylstilbestrol, hexestrol and the like can be used.
  • the aforementioned compounds alone or 2 or more compounds in combination can be used, with preference given to estradiol, diethylstilbestrol and the like.
  • non-steroidal estrogen receptor agonist tamoxifen citrate, toremifene citrate; raloxifene, arzoxifene, lasofoxifene, TSE-424, SERM-3339, SPC-8490 and the like can be used.
  • the mode of administration of the pharmaceutical agent for combined use of an LHRH receptor agonist or antagonist or a salt thereof with an androgen receptor agonist or a salt thereof is not particularly limited, as long as an LHRH receptor agonist or antagonist is combined with an androgen receptor agonist when administered.
  • Examples of such administration mode include (1) administration of a single preparation obtained by simultaneous formulation of an LHRH receptor agonist or antagonist and an androgen receptor agonist, (2) simultaneous administration by the same administration route of two kinds of preparations obtained by separate formulation of an LHRH receptor agonist or antagonist and an androgen receptor agonist, (3) staggered administration by the same administration route of two kinds of preparations obtained by separate formulation of an LHRH receptor agonist or antagonist and an androgen receptor agonist, (4) simultaneous administration by different administration routes of two kinds of preparations obtained by separate formulation of an LHRH receptor agonist or antagonist and an androgen receptor agonist, (5) staggered administration by different administration routes of two kinds of preparations obtained by separate formulation of an LHRH receptor agonist or antagonist and an androgen receptor agonist, such as administration in the order of an LHRH receptor agonist or antagonist and then an androgen receptor agonist, or in the reversed order, and the like.
  • antiandrogen drug and antiestrogen drug steroidal or non-steroidal antiandrogen agents and antiestrogen agents can be used.
  • antiandrogen drug for example, flutamide, bicalutamide, nilutamide and the like
  • antiestrogen drug for example, tamoxifen citrate, toremifene citrate; SERM agents (selective estrogen receptor modulator) such as raloxifene, arzoxifene, lasofoxifene, TSE-424, SERM-3339 or SPC-8490 and the like
  • SERM agents selective estrogen receptor modulator
  • raloxifene such as raloxifene, arzoxifene, lasofoxifene, TSE-424, SERM-3339 or SPC-8490 and the like
  • ER down regulator and the like can be used.
  • the following pharmaceutical agents known to have an antiandrogen or antiestrogen action and the like can be used.
  • antiandrogen drug and antiestrogen drug in the present application moreover, for example, fosfestrol, diethylstilbestrol, chlorotrianisene, medroxyprogesterone acetate, megestrol acetate, chlormadinone acetate, cyproterone acetate, danazol, allylestrenol, gestrinone, mepartricin, raloxifene, ormeloxifene, levormeloxifene, mepitiostane, testrolactone, aminoglutethimide, droloxifene, epitiostanol, ethynylestradiol sulfonate, aromatase inhibitors (e.g., fadrozole hydrochloride, anastrozole, letrozole, Excemestane, vorozole, formestane, finzorole and the like), 5 ⁇ -reductase inhibitors (e.g., 5
  • steroidal compounds for example, the compounds described in WO 92/15404, WO 93/20097, EP-A288053, EP-A413270 and the like can be used, and as the non-steroidal compounds, for example, (1H-imidazol-1-yl)methyl substituted benzimidazole derivative disclosed in JP-A-64-85975, carbazole derivatives disclosed in WO 94/27989, WO 96/14090 and WO 97/00257, azole derivative disclosed in WO 95/09157, 1H-benzimidazole derivative disclosed in U.S. Pat. No.
  • antiandrogen agent preferred are bicalutamide, flutamide and the like.
  • antiestrogen drug preferred are tamoxifen, toremifene, anastrozole, letrozole and the like.
  • hormone-dependent disease examples include sex hormone dependent cancers (e.g., prostate cancer, uterine cancer, breast cancer, pituitary gland tumor and the like), prostatic hypertrophy, endometriosis, hysteromyoma, precocious puberty, dysmenorrhea, amenorrhea, premenstrual syndrome, multilocular ovarian syndrome, postoperative recurrence of the aforementioned cancers, dwarfism, Alzheimer's disease, climacteric disturbance, indefinite complaints, metastasis of the aforementioned cancers, sex hormone-dependent diseases such as calcium.phosphorus bone metabolism disorder and the like and contraception (or infertility when a rebound effect after drug withdrawal is utilized), benign or malignant tumor which is sex hormone non-dependent but LHRH sensitive, and the like can be mentioned.
  • sex hormone dependent cancers e.g., prostate cancer, uterine cancer, breast cancer, pituitary gland tumor and the like
  • prostatic hypertrophy
  • the pharmaceutical agent of the present invention can be used for the prophylaxis or treatment of the aforementioned hormone-dependent diseases, it is particularly preferably used for the prophylaxis or treatment of sex hormone-dependent cancer, more preferably for the prophylaxis or treatment of prostate cancer (including lymph metastatic prostate cancer and bone metastatic prostate cancer), uterine cancer and breast cancer.
  • the object of treating cancer naturally means disappearance and cure of a malignant tumor, which includes reduction and maintenance of the volume of a malignant tumor and the like.
  • Such treatment objects are appropriately determined in medical situations.
  • Mammal refers to vertebrata that is warm-blooded and lung-breathing and includes, for example, human, simian, mouse, rat, swine, bovine, sheep, dog, horse and the like.
  • the bone metastatic prostate cancer refers to a malignant tumor that spreads to a bone tissue where, for example, MDA PCa 2b has been established.
  • a prostate cancer cell highly sensitive to androgen can be obtained by culturing prostate cancer cell (e.g., LNCaP-FGC and MDA PCa 2b cell lines (these are available from the ATCC) and the like) in a culture medium containing a charcoal treated serum. Culture is preferably continued for 3-8 months.
  • prostate cancer cell e.g., LNCaP-FGC and MDA PCa 2b cell lines (these are available from the ATCC) and the like
  • Culture is preferably continued for 3-8 months.
  • a breast or uterine cancer cell highly sensitive to estrogen can be obtained by culturing a breast or uterine cancer cell (e.g., MCF-7 cell line (these are available from the ATCC) and the like) in a culture medium containing a charcoal treated serum. Culture is preferably continued for 3-8 months.
  • a breast or uterine cancer cell e.g., MCF-7 cell line (these are available from the ATCC) and the like
  • MCF-7 cell line (these are available from the ATCC) and the like
  • Culture is preferably continued for 3-8 months.
  • the androgen sensitivity of cancer cell can be measured by determining the reactivity of cancer cell to an androgen receptor agonist.
  • the cancer cell can be measured by culturing the cancer cell in the presence of an androgen.
  • an androgen When the growth of cancer cell is promoted in an environment corresponding to normal testosterone concentration in living organisms (though subject to change depending on the measurement method, for example, 2.5-11 ng/ml: Guide to clinical examination '95, Bunkodo), it is assumed to be typical sensitivity, and when the growth of cancer cell is suppressed in this environment or the growth is promoted in an environment corresponding to the testosterone concentration during sterilization, it is assumed that the androgen sensitivity is enhanced.
  • the estrogen sensitivity of cancer cell can be measured by determining the reactivity of cancer cell to an estrogen receptor agonist.
  • the cancer cell can be measured by culturing the cancer cell in the presence of an estrogen.
  • an estrogen When the growth of cancer cell is promoted in an environment corresponding to normal estrogen concentration in living organisms (though subject to change depending on the measurement method, for example, 20-500 pg/ml in the case of estradiol: Guide to clinical examination '95, Bunkodo), it is assumed to be typical sensitivity, and when the growth of cancer cell is suppressed in this environment or the growth is promoted in an environment corresponding to the estrogen concentration during menopause, it is assumed that the estrogen sensitivity is enhanced.
  • the important information to determine the timing of change in the administration of a pharmaceutical agent and the like can be decided by determining a therapeutic effect based on the measurement of the size and the like of prostate (tumor), breast tumor and uterine tumor, by rectal test, palpation, ultrasonic test, magnetic resonance imaging (MRI), X ray, intravenous pyelography, CAT scanning, biopsy and the like.
  • prostate cancer can be easily determined by detecting PSA (prostatic specific antigen), which is a prostate specific antigen.
  • PSA prostate specific antigen
  • each drug is administered in the following amounts, which can be used in an appropriately increased or decreased amount depending on the pharmaceutical agent to be used in combination, dosage form, condition, administration period and the like, and which can be administered once a day or in several portions a day.
  • a single dose of the LHRH receptor agonist can be appropriately selected from the range of, for example, about 0.01 mg to 100 mg/kg body weight, preferably about 0.02 mg to 50 mg/kg body weight, more preferably 0.05 mg to 20 mg/kg body weight.
  • an LHRH receptor agonist is subcutaneously or intramuscularly administered in a single dose of generally about 0.01 to about 50 mg, preferably about 0.1 to about 20 mg, more preferably about 0.1 to about 15 mg.
  • an injection comprising a sustained-release microcapsule comprising the above-mentioned LHRH receptor agonist is administered, the dose varies depending on the drug release period of a sustained-release microcapsule.
  • a single dose of generally about 0.01 to about 20 mg, preferably about 0.1 to about 10 mg, more preferably about 0.1 to about 5 mg, of an LHRH receptor agonist is subcutaneously or intramuscularly administered.
  • a single dose of generally about 0.1 to about 30 mg, preferably about 0.1 to about 20 mg, more preferably about 1 to about 15 mg, of an LHRH receptor agonist is subcutaneously or intramuscularly administered.
  • the dose of an LHRH receptor antagonist is preferably about 1-3000 ⁇ g/kg/day, more preferably about 1-2000 ⁇ g/kg/day, more preferably about 1-1000 ⁇ g/kg/day.
  • the dose of an androgen receptor agonist is 0.001 mg-2000 mg/kg/day, preferably about 0.01-500 mg, more preferably about 0.01-100 mg, which is generally administered in 1 to 4 portions a day.
  • the dose of an estrogen receptor agonist is 0.001 mg-2000 mg/kg/day, preferably about 0.01-500 mg, more preferably about 0.01-100 mg, which is generally administered in 1 to 4 portions a day.
  • the dose of an antiandrogen drug is 0.001 mg-2000 mg/kg/day, preferably about 0.01-500 mg, more preferably about 0.01-100 mg, which is generally administered in 1 to 4 portions a day.
  • the dose of an antiestrogen drug is 0.001 mg-2000 mg/kg/day, preferably about 0.01-500 mg, more preferably about 0.01-100 mg, which is generally administered in 1 to 4 portions a day.
  • the pharmaceutical agent of the present invention can be produced by adding an LHRH receptor agonist or antagonist, an androgen receptor agonist, an estrogen receptor agonist, an antiandrogen drug or antiestrogen drug and the like in generally 0.1-95% (w/w) of the total amount of the preparation according to a conventional method.
  • cancer by combining 1 to 3 kinds selected from the group consisting of (1) administering an effective amount of the pharmaceutical agent of the present invention, (2) (i) administering an effective amount of other anticancer agents, (ii) administering an effective amount of other hormone therapy agents, and (iii) therapies without medicine, cancer can be more effectively prevented or treated.
  • therapy without medicine for example, operation, radiation therapy, gene therapy, thermotherapy, cryotherapy, laser cauterization therapy and the like can be mentioned, and two or more of these can be combined.
  • the compound of the present invention can be used in combination with other hormone therapy agents, anticancer agents (e.g., chemotherapy agent, immunotherapy agent and pharmaceutical agent inhibiting the action of cell growth factor and receptor thereof) and the like (hereinafter to be briefly referred to as combination drug) [hereinafter to be briefly referred to as combination agent].
  • anticancer agents e.g., chemotherapy agent, immunotherapy agent and pharmaceutical agent inhibiting the action of cell growth factor and receptor thereof
  • combination drug hereinafter to be briefly referred to as combination drug
  • the compound of the present invention shows a superior anticancer effect even when used as a single agent, when it is used in combination with one or more of the above-mentioned combination drugs (combined use of plural agents), the effect can be reinforced furthermore.
  • the pharmaceutical agent of the present invention or the combination agent of the present invention may be combined with a therapy without medicine, such as (1) operation, (2) vasopressor chemotherapy using angiotensin II, (3) gene therapy, (4) thermotherapy, (5) cryotherapy, (6) laser cauterization therapy, (7) radiation therapy and the like.
  • a therapy without medicine such as (1) operation, (2) vasopressor chemotherapy using angiotensin II, (3) gene therapy, (4) thermotherapy, (5) cryotherapy, (6) laser cauterization therapy, (7) radiation therapy and the like.
  • the use of the pharmaceutical agent of the present invention or the combination agent of the present invention before or after operation and the like, or before or after a combined treatment of two or three kinds thereof affords effects such as prevention of expression of resistance, prolonged Disease-Free Survival time, suppression of metastasis or recurrence, life prolonging and the like.
  • a therapy with the pharmaceutical agent of the present invention or the combination agent of the present invention, with a supportive therapy such as (i) administration of antibiotics (e.g., ⁇ -lactam group such as pansporin, macrolide group such as clarithromycin etc.) against complication with various infectious diseases, (ii) administration of total parenteral nutrition, amino acid preparation, multivitamin preparation for improvement of malnutrition, (iii) administration of morphine for relieving pain, (iv) administration of medicine to improve side effects such as nausea, vomit, anorexia, diarrhea, hypoleukocytemia, thrombocytopenia, decreased hemoglobin concentration, alopecia, hepatopathy, nephropathy, DIC, onset of fever and the like, and (v) administration of medicine to suppress multiple drug resistance in cancer, and the like.
  • antibiotics e.g., ⁇ -lactam group such as pansporin, macrolide group such as clarithromycin etc.
  • a supportive therapy such as (i
  • the pharmaceutical agent of the present invention or an agent for the prophylaxis or treatment of the present invention be administered before or after the aforementioned treatment by oral administration (inclusive of sustained release administration), intravenous administration (inclusive of bolus, infusion and inclusion compound), subcutaneous or intramuscular injection (inclusive of bolus, infusion, sustained release administration), transdermal, intra-tumor and proximal administrations.
  • the pharmaceutical agent of the present invention or the combination agent of the present invention when administered before operation and the like, for example, it may be administered once in about 30 min. to 24 hrs. before the operation and the like, or may be administered in 1 to 3 cycles in about 3 to 6 months before the operation and the like.
  • the administration of the pharmaceutical agent of the present invention or the combination agent of the present invention before operation and the like can, for example, shrink cancer tissues, thereby facilitating the operation and the like.
  • the pharmaceutical agent of the present invention or the combination agent of the present invention When the pharmaceutical agent of the present invention or the combination agent of the present invention is administered after operation and the like, it may be administered repeatedly in about 30 min to 24 hrs. after the operation and the like, for example, for several weeks to 3 months.
  • the administration of the pharmaceutical agent of the present invention or the combination agent of the present invention after operation and the like enhances the effect of the operation and the like.
  • the treatment method of the present invention includes
  • an effective amount of an LHRH receptor agonist or antagonist or a salt thereof for a certain period (e.g., 3 months to 5 years), androgen sensitivity of the prostate cancer cell is enhanced. Thereafter, by administering an effective amount of an androgen receptor agonist or a salt thereof, the growth of the prostate cancer cell can be suppressed or the tumor can be shrunk.
  • a most suitable treatment of prostate cancer can be performed by changing to an administration of 1 or 2 compounds selected from an LHRH receptor agonist or antagonist and an antiandrogen drug, or a salt thereof, after which selecting based on such androgen sensitivity of the cancer cell and changing to an administration of 1 or 2 compounds selected from an LHRH receptor agonist or antagonist and an antiandrogen drug, or a salt thereof (when androgen sensitivity of the cancer cell is of the same level as normal cells [e.g., LNCaP 104-S cell (Cancer Res, 54, p 1566-1573), LNCaP-FGC cell and the like]) or an administration of androgen receptor agonist or a salt thereof (when androgen sensitivity of the cancer cell is higher than normal cells [e.g., LNCaP 104-R2 cell ( Cancer Res
  • the timing of changing these administrations can be appropriately set for each treatment, and, for example, from 3 months to 5 years, preferably from 6 months to 4 years, more preferably from 1 year to 3 years, still more preferably from 1 year to 2 years.
  • MAB Maximum androgen blockade
  • LHRH receptor agonist or antagonist e.g., LHRH receptor agonist and the like
  • a treatment method based on a combined use of the androgen receptor agonist or a salt thereof of the present invention proves effective.
  • an androgen receptor agonist can be administered while continuing the administration of an LHRH receptor agonist or antagonist (whereby a condition similar to an intermittent therapy with LHRH receptor agonist or antagonist can be performed while continuing administration of an LHRH receptor agonist or antagonist), or administration of an LHRH receptor agonist or antagonist may be stopped and changed to the administration of an androgen receptor agonist. Both cases are encompassed in the present invention.
  • the androgen sensitivity of prostate cancer cell can be measured by the aforementioned method for determining the reactivity with androgen, it can be also assumed based on the increase or decrease of tumor marker, physiological index, tumor volume and the like under administration of a certain drug.
  • the treatment method of the present invention includes
  • estrogen sensitivity of the breast or uterine cancer cell is enhanced. Thereafter, by administering an effective amount of an estrogen receptor agonist or a salt thereof, the growth of the breast or uterine cancer cell can be suppressed or the tumor can be shrunk.
  • a most suitable treatment of breast or uterine cancer can be performed by changing to an administration of 1 or 2 compounds selected from an LHRH receptor agonist or antagonist and an antiestrogen drug, or a salt thereof, after which selecting based on such estrogen sensitivity of the cancer cell and changing to an administration of 1 or 2 compounds selected from an LHRH receptor agonist or antagonist and an antiestrogen drug, or a salt thereof (when estrogen sensitivity of cancer cell is of the same level as normal cells) or an administration of estrogen receptor agonist or a salt thereof (when estrogen sensitivity of the cancer cell is higher than normal cells).
  • the timing of changing these administrations can be appropriately set for each treatment, and, for example, from 3 months to 5 years, preferably from 6 months to 4 years, more preferably from 1 year to 3 years, still more preferably from 1 year to 2 years.
  • LHRH receptor agonist or antagonist e.g., LHRH receptor agonist and the like
  • the estrogen sensitivity of breast or uterine cancer cell is highly likely to have been enhanced, and a treatment method based on a combined use of the estrogen receptor agonist or a salt thereof of the present invention proves effective.
  • an estrogen receptor agonist can be administered while continuing the administration of an LHRH receptor agonist or antagonist, or administration of an LHRH receptor agonist or antagonist may be stopped and changed to the administration of an estrogen receptor agonist. Both cases are encompassed in the present invention.
  • estrogen sensitivity of breast or uterine cancer cell can be measured by the aforementioned method for determining the reactivity with estrogen, it can be also assumed based on the increase or decrease of tumor marker, physiological index, tumor volume and the like under administration of a certain drug.
  • LHRH receptor agonist or antagonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • LHRH receptor agonist or antagonist can be administered orally as a tablet sugar coated as necessary, a capsule, an elixir, a sustained-release preparation and the like, or parenterally in the form of an injection such as a sterile solution, a suspension, a sustained-release preparation (particularly a sustained-release microcapsule) and the like with water or other pharmacologically acceptable solution, an embedded agent (a formed product with a biodegradable polymer as a base, one that is sealed in a tube of a biologically compatible metal such as titanium and the like and releases the active ingredient at a predetermined speed), an injection wherein a biodegradable polymer and a drug are dissolved or dispersed in an organic solvent administrable to living organisms, or a transnasal administration preparation of a solution, a suspension and the
  • sustained-release preparation is preferably administered as a sustained-release preparation, particularly preferably as a sustained-release injection.
  • sustained-release preparation is a sustained-release microcapsule, it is preferably a long-term sustained-release microcapsule that releases an LHRH receptor agonist or antagonist over the period of 2 months or longer.
  • the aforementioned preparation can be produced by admixing leuprorelin or a salt thereof, more preferably leuprorelin acetate with a physiologically known carrier, flavor, excipient, vehicle, preservative, stabilizer, binder and the like in a unit dosage form required for generally admitted preparation practice.
  • binders such as gelatin, cornstarch, tragacanth and gum arabic
  • excipients such as crystalline cellulose
  • swelling agents such as cornstarch, gelatin, alginic acid and the like
  • lubricants such as magnesium stearate
  • sweeteners such as sucrose, lactose and saccharin
  • flavors such as peppermint, akamono oil and cherry, and the like
  • the unit dosage form is a capsule
  • the aforementioned type of materials can further contain a liquid carrier such as fats and oils.
  • a sterile composition for injection can be produced according to conventional preparation practice such as dissolving or suspending an active substance, naturally occurring vegetable oil such as sesame oil, palm oil and the like, and the like in a vehicle such as water for injection, and the like.
  • aqueous solution for injection for example, isotonic solutions (e.g., D-sorbitol, D-mannitol, sodium chloride and the like) containing physiological saline, glucose and other auxiliary drugs and the like are used, which may be used concurrently with suitable dissolution aids, such as alcohol (e.g., ethanol), polyalcohol (e.g., propylene glycol, polyethylene glycol), non-ionic surfactants (e.g., polysorbate 80TM, HCO-50) and the like.
  • suitable dissolution aids such as alcohol (e.g., ethanol), polyalcohol (e.g., propylene glycol, polyethylene glycol), non-ionic surfactants (e.g., polysorbate
  • the aforementioned preparation may be blended with, for example, buffers (e.g., phosphate buffer, sodium acetate buffer), soothing agents (e.g., benzalkonium chloride, procaine hydrochloride and the like), stabilizers (e.g., human serum albumin, polyethylene glycol and the like), preservatives (e.g., benzyl alcohol, phenol and the like), antioxidants and the like.
  • buffers e.g., phosphate buffer, sodium acetate buffer
  • soothing agents e.g., benzalkonium chloride, procaine hydrochloride and the like
  • stabilizers e.g., human serum albumin, polyethylene glycol and the like
  • preservatives e.g., benzyl alcohol, phenol and the like
  • antioxidants e.g., benzyl alcohol, phenol and the like.
  • a sustained-release preparation (particularly a sustained-release microcapsule) containing the aforementioned LHRH receptor agonist or antagonist (preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate) can be produced according to a known method such as a method described in, for example, JP-A-60-100516, JP-A-62-201816, JP-A-4-321622, JP-A-6-192068, JP-A-9-132524, JP-A-9-221417, JP-A-11-279054, WO 99/360099 and the like.
  • sustained-release preparations “a long-term sustained-release microcapsule designed for zero order release of a physiological active substance over 2 months or longer” described in JP-A-4-321622 is particularly preferably used.
  • an LHRH receptor agonist or antagonist (preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate) is dissolved in water at about 20% to 70% (W/W), preferably 25-65% (W/W), more preferably 35-60% (W/W), and, if necessary, gelatin, or a drug retaining substance such as basic amino acid and the like is dissolved or suspended therein to give an internal aqueous phase.
  • the internal aqueous phase may be supplemented with a pH regulator for retaining stability or solubility of an LHRH receptor agonist or antagonist (e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate), such as carbonic acid, acetic acid, oxalic acid, citric acid, phosphoric acid, hydrochloric acid, sodium hydroxide, arginine, lysine or a salt thereof.
  • LHRH receptor agonist or antagonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • an LHRH receptor agonist or antagonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • albumin e.g., albumin, gelatin, citric acid, sodium ethylenediaminetetraacetate, dextrin, sodium hydrogen sulfite, polyol compounds such as polyethylene glycol and the like, or p-oxybenzoic acid esters (methylparaben, propylparaben and the like) conventionally used as preservatives, benzyl alcohol, chlorobutanol, thimerosal and the like may be added.
  • LHRH receptor agonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • albumin e.g., albumin, gelatin, citric acid, sodium ethylenediaminetetraacetate, dextrin, sodium hydrogen sulfit
  • the internal aqueous phase thus obtained is added to a solution (oil phase) containing a polymer and emulsified to give a W/O emulsion.
  • a known dispersing method is used, and, for example, an intermittent shaking method, a method using a mixer such as a propeller stirrer, a turbine stirrer and the like, a colloidal mill method, a homogenizer method, an ultrasonication method and the like are used.
  • microcapsulation step For this step, an in-water drying method or a phase separation method can be applied.
  • the W/O emulsion is further added to a third aqueous phase to form a W/O/W three-phase emulsion and a solvent in the oily phase is evaporated to give a microcapsule.
  • An emulsifier may be added to the aforementioned external aqueous phase, and an example thereof may be any as long as it forms a generally stable O/W emulsion.
  • anion surfactants sodium oleate, sodium stearate, sodium lauryl sulfate and the like
  • non-ionic surfactants sorbitan polyoxyethylene fatty acid ester [Tween 80, Tween 60, Atlas Powder], polyoxyethylene castor oil derivatives [HCO-60, HCO-50, Nikko Chemicals] and the like
  • polyvinylpyrrolidone polyvinyl alcohol, carboxymethyl cellulose, lecithin, gelatin and the like can be mentioned, wherein one or more kinds thereof may be used in combination.
  • concentration during use is appropriately selected from the range of about 0.01% to 20%, more preferably about 0.05% to 10%.
  • a method generally used for evaporation of the solvent from an oily phase. Such method includes gradual depressurization while stirring with a propeller stirrer, magnetic stirrer and the like, or evaporation using a rotary evaporator and the like while controlling the degree of vacuum.
  • a W/O/W emulsion is gradually heated to more completely perform desorption of the solvent, whereby the necessary time can be shortened.
  • microcapsule thus obtained is subjected to centrifugal separation or filtration and separated, and free LHRH receptor agonist or antagonist (preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate), a drug retaining substance, an emulsifier and the like adhered to the surface of microcapsule are repeatedly washed several times with distilled water, dispersed again in distilled water and the like and lyophilized.
  • a coagulation preventive e.g., mannitol, sorbitol, lactose, glucose and the like
  • Heating is applied if necessary, whereby water and organic solvent in microcapsule is more completely removed under reduced pressure.
  • a coacervating agent is gradually added to the w/o emulsion while the emulsion is stirred, to precipitate and solidify a polymer.
  • Any coacervating agent can be used, as long as it is a polymeric, mineral oil or vegetable oil compound miscible with the solvent for a polymer and that does not dissolve a polymer for capsulation.
  • coacervating agents include silicon oil, sesame oil, soybean oil, corn oil, cotton seed oil, coconut oil, linseed oil, mineral oil, n-hexane and n-heptane. Two or more of these may be used in combination.
  • microcapsule thus obtained is filtrated, separated, washed repeatedly with heptane and the like and a coacervating agent is removed.
  • a free drug is removed by a method similar to an in-water drying method and the solvent is eliminated.
  • a coagulation preventive may be added to prevent coagulation of particles during washing.
  • the microcapsule obtained above is lightly pulverized, if necessary, passed through a sieve, thereby removing microcapsules having large sizes.
  • the particle size of the microcapsule is desirably in the range of about 0.5-1000 ⁇ m in an average diameter, more preferably in the range of about 2-500 ⁇ m.
  • the range may be any as long as it can be dispersed and pass through a needle. For example, it is desirably in the range of about 2 to 100 ⁇ m.
  • the aforementioned polymer include, for example, biodegradable polymers such as polymers and copolymers that have been synthesized from one or more kinds of ⁇ -hydroxycarboxylic acids selected from ⁇ -hydroxymonocarboxylic acids (e.g., glycolic acid, lactic acid), ⁇ -hydroxydicarboxylic acids (e.g., malic acid), ⁇ -hydroxytricarboxylic acids (e.g., citric acid) etc., and that have a free carboxyl group, or mixtures thereof; poly- ⁇ -cyanoacrylic acid esters; polyamino acids (e.g., poly- ⁇ -benzyl-L-glutamic acid); and maleic anhydride copolymers (e.g., styrene-maleic acid copolymers).
  • biodegradable polymers such as polymers and copolymers that have been synthesized from one or more kinds of ⁇ -hydroxycarboxylic acids selected from ⁇ -hydroxymonocarboxylic acids (
  • the mode of monomer binding may be random, block, or graft.
  • ⁇ -hydroxymonocarboxylic acids, ⁇ -hydroxydicarboxylic acids, and ⁇ -hydroxytricarboxylic acids have an optically active center in their molecular structures, they may be of the D-, L- or DL-configuration.
  • lactic acid-glycolic acid polymers [hereinafter also referred to as poly(lactide-co-glycolide), poly(lactic acid-co-glycolic acid) or lactic acid-glycolic acid copolymer; generically refer to lactic acid-glycolic acid homopolymers and copolymers, unless otherwise specified; lactic acid homopolymers are also referred to as lactic acid polymer, polylactic acids, polylactides etc., and glycolic acid homopolymers as glycolic acid polymers, polyglycolic acids, polyglycolides etc.], with preference given to poly( ⁇ -cyanoacrylic esters) etc. Greater preference is given to lactic acid-glycolic acid polymers. More preferably, lactic acid-glycolic acid polymers having a free carboxyl group at one end are used.
  • the biodegradable polymer may be a salt.
  • Salts include, for example, salts with inorganic bases (e.g., alkali metals such as sodium and potassium, alkaline earth metals such as calcium and magnesium), organic bases. (e.g., organic amines such as triethylamine, basic amino acids such as arginine), and salts and complex salts with transition metals (e.g., zinc, iron, copper).
  • inorganic bases e.g., alkali metals such as sodium and potassium, alkaline earth metals such as calcium and magnesium
  • organic bases e.g., organic amines such as triethylamine, basic amino acids such as arginine
  • salts and complex salts with transition metals e.g., zinc, iron, copper.
  • the composition ratio (mol %) is preferably about 100/0 to about 40/60, more preferably about 100/0 to about 50/50.
  • a lactic acid homopolymer having a composition ratio of 100/0 is preferably used.
  • the optical isomer ratio of lactic acid, one of the minimum repeat units for said “lactic acid-glycolic acid polymer” is preferably between about 75/25 and about 25/75, as of the D-configuration/L-configuration ratio (mol/mol %). Lactic acid-glycolic acid polymers having a D-configuration/L-configuration ratio (mol/mol %) between about 60/40 and about 30/70 are commonly used.
  • the weight-average molecular weight of said “lactic acid-glycolic acid polymer” is normally about 3,000 to about 100,000, preferably about 3,000 to about 60,000, more preferably about 3,000 to about 50,000.
  • the degree of dispersion is normally about 1.2 to about 4.0, more preferably about 1.5 to 3.5.
  • the free carboxyl group content of said “lactic acid-glycolic acid polymer” is preferably about 20 to about 1,000 ⁇ mol (micromol), more preferably about 40 to about 1,000 ⁇ mol (micromol), per unit mass (gram) of the polymer.
  • Weight-average molecular weight, number-average molecular weight and degree of dispersion, as defined herein, are polystyrene-based molecular weights and degree of dispersion determined by gel permeation chromatography (GPC) with 15 polystyrenes as reference substances with weight-average molecular weights of 1,110,000, 707,000, 455, 645, 354,000, 189,000, 156,055, 98,900, 66,437, 37,200, 17,100, 9,830, 5,870, 2,500, 1,303, and 504, respectively.
  • GPC gel permeation chromatography
  • Measurements were taken using a high-speed GPC device (produced by Toso, HLC-8120GPC, detection: Refractory Index) and a GPC column KF804Lx2 (produced by Showa Denko), with chloroform as a mobile phase.
  • the flow rate is 1 ml/min.
  • free carboxyl group content is defined to be obtained by the labeling method (hereinafter referred to as “carboxyl group content as determined by the labeling method”). Specific procedures for determining this content in a polylactic acid are described below.
  • ONPH o-nitrophenylhydrazine hydrochloride
  • said “carboxyl group content” can also be obtained by dissolving the biodegradable polymer in a toluene-acetone-methanol mixed solvent, and titrating this solution for carboxyl groups with an alcoholic solution of potassium hydroxide, with phenolphthalein as indicator (value obtained by this method hereinafter referred to as “carboxyl group content as determined by the alkali titration method”), it is desirable that quantitation be achieved by the labeling method described above, since it is possible that the titration endpoint is made unclear as a result of competition of the hydrolytic reaction of the polyester main chain during titration.
  • Said “lactic acid-glycolic acid polymer” can be produced by, for example, the catalyst-free dehydration polymerization condensation method (JP-A-61-28521) from a lactic acid and a glycolic acid, or ring-opening polymerization from a lactide and a cyclic diester compound such as glycolide by means of a catalyst ( Encyclopedic Handbook of Biomaterials and Bioengineering Part A: Materials ; Volume 2, Marcel Dekker, Inc., 1995).
  • the polymer obtained by the above-mentioned known method of ring-opening polymerization does not always contain a free carboxyl group at one end, it can also be used after being modified to a polymer having a given amount of carboxyl groups per unit mass, by subjecting it to the hydrolytic reaction described in EP-A-0839525.
  • lactic acid-glycolic acid polymer having a free carboxyl group at one end can be produced by a commonly known method (e.g., catalyst-free dehydration polymerization condensation, JP-A-61-28521), or by a method analogous thereto.
  • the microcapsules are dispersed in an aqueous medium together with a dispersing agent (e.g. Tween 80, HCO-60, carboxymethylcellulose, sodium alginate, etc.), a preservative (e.g. methylparaben, propylparaben, etc.), an isotonizing agent (e.g. sodium chloride, mannitol, sorbitol, glucose, etc.), or suspended in an aqueous medium together with a vegetable oil such as sesame oil or corn oil.
  • a dispersing agent e.g. Tween 80, HCO-60, carboxymethylcellulose, sodium alginate, etc.
  • a preservative e.g. methylparaben, propylparaben, etc.
  • an isotonizing agent e.g. sodium chloride, mannitol, sorbitol, glucose, etc.
  • a vegetable oil such as sesame oil or corn oil.
  • the aforementioned agent comprising an LHRH receptor agonist or antagonist e.g., an LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • it can be formulated into the aforementioned various preparations and administered, and can be also used as a starting material substance to produce such preparation.
  • the dose of the aforementioned preparation may vary depending on the amount of LHRH receptor agonist or antagonist (e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate), dosage form, duration of release of LHRH receptor agonist or antagonist (e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate), recipient animal [e.g., warm-blooded mammal (e.g., human, mouse, rat, rabbit, sheep, swine, bovine, horse and the like)] but should be within the range of an effective dose of said LHRH receptor agonist or antagonist (e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate) as a pharmaceutical agent.
  • the single dose per said animal can adequately be selected within the range of about 0.01 mg to 100 mg/kg body weight,
  • an LHRH receptor agonist or antagonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • LHRH receptor agonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • the dose varies depending on the duration of the drug release of the sustained-release microcapsule, wherein, for example, for administration for once in about 1 month, generally about 0.01 to about 20 mg, preferably about 0.1 to about 10 mg, more preferably about 0.1 to about 5 mg of an LHRH receptor agonist or antagonist (e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate) only needs to be administered subcutaneously or intramuscularly for one dose an adult patient (body weight 60 kg) with prostate cancer, and, for example, for administration for once in about 3 months, generally about 0.1 to about 30 mg, preferably about 0.1 to about 20 mg, more preferably about 0.1 to about 15 mg
  • LHRH receptor agonist or antagonist e.g., LHRH receptor agonist, preferably leuprorelin or a salt thereof, more preferably leuprorelin acetate
  • an amount converted based on the body weight of 60 kg can be administered.
  • the Add-Back therapy means a treatment method employed when prophylaxis or treatment of a disease that exacerbates depending on sex hormone (testosterone, estrogen, estradiol and the like) in blood is desired by administering an LHRH receptor agonist to reduce these hormones, which comprises auxiliarily administering these hormone or pharmaceutical agents (e.g., SERM agents or SARM agents free of action on prostate cancer and breast cancer; hereinafter sometimes to be abbreviated as an Add-Back agent) that is considered to be equivalent to these hormones and the like, thereby to reduce these hormones, or alleviate the side effects (e.g., lower bone mass) caused by the efficacy.
  • the Add-Back agents are mainly preferably administered by oral administration.
  • the MAB therapy is a treatment method to block any androgen action in the prostate gland.
  • it refers to a surgical sterilization to block actions of androgen derived from testicular or a treatment method concurrently using an LHRH receptor agonist or antagonist and antiandrogen to block actions of an adrenogenic androgen.
  • an LHRH receptor agonist or antagonist for the combined use of an LHRH receptor agonist or antagonist, an androgen receptor agonist and combination drug(s), the time of administration of an LHRH receptor agonist or antagonist, an androgen receptor agonist and combination drug(s) is not limited.
  • the LHRH receptor agonist or antagonist and the androgen receptor agonist or a preparation thereof and the combination drug(s) or a preparation thereof may be simultaneously administered to an administration object or administered in a staggered manner.
  • the dose of the combination drug only needs to follow the dose clinically employed, and can be determined as appropriate depending on the administration object, administration route, disease, combination and the like.
  • the mode of administration of the pharmaceutical agent comprising the LHRH receptor agonist or antagonist and the androgen receptor agonist in combination with the combination drug is not particularly limited, and may be any as long as the LHRH receptor agonist or antagonist and the androgen receptor agonist and combination drug(s) are combined on administration.
  • Such administration mode is exemplified by (1) administration of a single preparation obtained by simultaneously formulating the LHRH receptor agonist or antagonist and the androgen receptor agonist, and combination drug(s) into a preparation, (2) simultaneous administration by the same administration route of two kinds of preparations obtained by separately formulating the LHRH receptor agonist or antagonist and the androgen receptor agonist, and combination drug(s) into preparations, (3) staggered administration by the same administration route of two kinds of preparations obtained by separately formulating the LHRH receptor agonist or antagonist and the androgen receptor agonist, and combination drug(s) into preparations, (4) simultaneous administration by different administration routes of two kinds of preparations obtained by separately formulating the LHRH receptor agonist or antagonist and the androgen receptor agonist, and combination drug(s) into preparations, (5) staggered administration by different administration routes of two kinds of preparations obtained by separately formulating the LHRH receptor agonist or antagonist and the androgen receptor agonist, and combination drug(s) into preparations (e.g., administration in the order of the
  • the 1) pharmaceutical agent comprising a LHRH receptor agonist or antagonist or a salt thereof in combination with an androgen receptor agonist or a salt thereof, 2) agent for the prophylaxis or treatment of bone metastatic prostate cancer comprising an androgen receptor agonist, 3) agent for the prophylaxis or treatment of prostate cancer comprising a non-steroidal androgen receptor agonist and 4) preparation thereof comprising the aforementioned combination drug(s) in combination of the present invention have low toxicity and can be administered safely by admixing these agent with, for example, a pharmacologically acceptable carrier according to a method known per se to give a pharmaceutical composition, such as tablets (inclusive of sugar-coated tablets and film-coated tablets), powders, granules, capsules, (inclusive of soft capsules), liquids, injections, suppositories, sustained release agents and the like, for oral or parenteral (e.g., topical, rectal or intravenous administration) administration.
  • An injection can be administered intravenously
  • the pharmacologically acceptable carrier usable for the production of the preparation there are mentioned various conventional organic or inorganic carriers as a material for the preparation.
  • various conventional organic or inorganic carriers include excipients, lubricants, binders and disintegrators for solid preparations, and solvents, solubilizing aids, suspending agents, isotonic agents, buffers and soothing agents for liquid preparations.
  • conventional additives such as antiseptics, antioxidants, coloring agents, sweeteners, absorbents, moistening agent and the like can be used.
  • excipient examples include lactose, sucrose, D-mannitol, starch, corn starch, crystalline cellulose, light anhydrous silicic acid and the like.
  • lubricant examples include magnesium stearate, calcium stearate, talc, colloidal silica and the like.
  • binder examples include crystalline cellulose, sucrose, D-mannitol, dextrin, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinylpyrrolidone, starch, sucrose, gelatin, methylcellulose, sodium carboxymethylcellulose and the like.
  • disintegrator examples include starch, carboxymethylcellulose, carboxymethylcellulose calcium, sodium carboxymethyl starch, L-hydroxypropylcellulose and the like.
  • solvent examples include injectable water, alcohol, propylene glycol, Macrogol, sesame oil, corn oil, olive oil and the like.
  • solubilizing aid examples include polyethylene glycol, propylene glycol, D-mannitol, benzyl benzoate, ethanol, tris-aminomethane, cholesterol, triethanolamine, sodium carbonate, sodium citrate and the like.
  • suspending agent examples include surfactants such as stearyl triethanolamine, sodium lauryl sulfate, lauryl aminopropionic acid, lecithin, benzalkonium chloride, benzethonium chloride, glyceryl monostearate and the like; hydrophilic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, sodium carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose and the like.
  • surfactants such as stearyl triethanolamine, sodium lauryl sulfate, lauryl aminopropionic acid, lecithin, benzalkonium chloride, benzethonium chloride, glyceryl monostearate and the like
  • hydrophilic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, sodium carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose and the like.
  • isotonicity agent examples include glucose, D-sorbitol, sodium chloride, glycerine, D-mannitol and the like.
  • buffers such as phosphate, acetate, carbonate, citrate and the like.
  • Examples of the soothing agent include benzyl alcohol and the like.
  • antiseptic examples include p-oxybenzoates, chlorobutanol, benzyl alcohol, phenethyl alcohol, dehydroacetic acid, sorbic acid and the like.
  • antioxidant examples include sulfite, ascorbic acid, ⁇ -tocopherol and the like.
  • the content of the combination drug in the preparation comprising combination drug(s) is generally about 0.01-100 wt %, preferably about 0.1-50 wt %, more preferably about 0.5-20 wt %, based on the preparation in total, though they may change depending on the preparation form.
  • the content of the additive such as carrier in the preparation comprising the combination drug varies depending on the form of the preparation. It is generally about 1-99.99 wt %, preferably about 10-90 wt %, based on the preparation in total.
  • a combination drug can be prepared into an aqueous injection together with a dispersant (e.g., Tween 80 (ATLASPOWDER USA), HCO60 (NIKKO CHEMICALS), polyethylene glycol, carboxymethylcellulose, sodium arginate, hydroxypropylmethylcellulose, dextrin etc.), a stabilizer (e.g., ascorbic acid, sodium pyrosulfite etc.), a surfactant (e.g., polysorbate 80, Macrogol etc.), a solubilizer (e.g., glycerine, ethanol etc.), a buffering agent (e.g., phosphoric acid, alkali metal salt thereof, citric acid, alkali metal salt thereof etc.), an isotonicity agent (e.g., sodium chloride, potassium chloride, mannitol, sorbitol, glucose etc.), a pH adjusting agent (hydrochloric acid, sodium hydroxide etc.), a preservative (e.g
  • glycerine, meglumine etc. a solubilizing aid
  • a solubilizing aid e.g., propylene glycol, sucrose etc.
  • a soothing agent e.g., glucose, benzyl alcohol etc.
  • an oil-based injection by dissolving, suspending or emulsifying in a vegetable oil (e.g., olive oil, sesame oil, cottonseed oil, corn oil etc.) or a solubilizing aid such as propylene glycol etc., and used as an injection.
  • a vegetable oil e.g., olive oil, sesame oil, cottonseed oil, corn oil etc.
  • a solubilizing aid such as propylene glycol etc.
  • An oral formulation can be produced by a method known per se by admixing a combination drug with an excipient (e.g., lactose, sucrose, starch and the like), a disintegrant (e.g., starch, calcium carbonate and the like), a binder (e.g., starch, gum arabic, carboxymethyl cellulose, polyvinyl pyrrolidone, hydroxypropyl cellulose and the like) or a lubricant (e.g., talc, magnesium stearate, polyethylene glycol 6000 and the like) and compressing the mixture, optionally followed by a coating process known per se for the purpose of masking a taste, forming an enteric coat, or achieving a sustained release.
  • an excipient e.g., lactose, sucrose, starch and the like
  • a disintegrant e.g., starch, calcium carbonate and the like
  • a binder e.g., starch, gum arabic,
  • Such coating may, for example, be hydroxypropylmethyl cellulose, ethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, polyoxyethylene glycol, Tween 80, Pluronic F68, cellulose acetate phthalate, hydroxypropylmethyl cellulose phthalate, hydroxymethyl cellulose acetate succinate, Eudragit (ROHME, Germany, a copolymer of methacrylic acid and acrylic acid), a dye (e.g., colcothar, titanium oxide etc.) and the like.
  • the preparation for oral administration may be either a rapid release preparation or a sustained release preparation.
  • a suppository can be produced by making a combination drug into an oily or aqueous solid, semisolid or liquid composition.
  • the oily base to be used for such a composition include glyceride of higher fatty acid (e.g., cacao butter, Witepsol (Dynamit Nobel, Germany etc.), medium fatty acid (e.g., miglyol (Dynamit Nobel, Germany etc.), vegetable oil (e.g., sesame oil, soybean oil, cottonseed oil etc.) and the like.
  • the aqueous base include polyethylene glycols, propylene glycol and the like.
  • the aqueous gel base include natural gums, cellulose derivative, vinyl polymer, acrylate polymer and the like.
  • sustained release preparation examples include sustained release microcapsule and the like.
  • a sustained release microcapsule can be prepared by a method known per se.
  • a sustained release preparation shown in the following [2] is preferably formed and administered.
  • An injection containing a combination drug dissolved in water is preferable.
  • the injection may contain benzoate and/or salicylate.
  • the injection is obtained by dissolving both a combination drug and, where desired, benzoate and/or salicylate in water.
  • the salt of the above-mentioned benzoic acid and salicylic acid includes, for example, alkali metal salts such as sodium, potassium and the like, alkaline earth metal salts such as calcium, magnesium and the like, ammonium salt, meglumine salt, and organic acid salt such as trometamol and the like, and the like.
  • the concentration of a combination drug in the injection is about 0.5-50 w/v %, preferably about 3-20 w/v %.
  • the concentration of the benzoate and/or salicylate is preferably 0.5-50 w/v %, more preferably 3-20 w/v %.
  • This agent may contain additives generally used for injections, such as a stabilizer (e.g., ascorbic acid, sodium pyrosulfite etc.), a surfactant (e.g., polysorbate 80, Macrogol etc.), a solubilizer (e.g., glycerine, ethanol etc.), a buffering agent (e.g., phosphoric acid, alkali metal salt thereof, citric acid, alkali metal salt thereof etc.), an isotonicity agent (e.g., sodium chloride, potassium chloride etc.), a dispersing agent (e.g., hydroxypropylmethylcellulose, dextrin), a pH adjusting agent (hydrochloric acid, sodium hydroxide etc.), a preservative (ethyl p-hydroxybenzoate, benzoic acid etc.), a solubilizer (e.g., conc.
  • a stabilizer e.g., ascorbic acid, sodium pyrosulfite
  • glycerine meglumine etc.
  • a solubilizing aid e.g., propylene glycol, sucrose etc.
  • a soothing agent e.g., glucose, benzyl alcohol etc.
  • the injection is preferably adjusted to pH 2-12, preferably 2.5-8.0, by the use of a pH adjusting agent.
  • the injection can be obtained by dissolving both LHRH receptor agonist or a combination drug and, where desired, benzoate and/or salicylate, and where necessary, the above-mentioned additives in water. These may be dissolved in any order in a suitable manner as in conventional production of injections.
  • the injectable aqueous solution is preferably heated and, in the same manner as with conventional injections, subjected to, for example, sterilization by filtration, high pressure sterilization by heating and the like to provide an injection.
  • the injectable aqueous solution is preferably subjected to high pressure sterilization by heating at, for example, 100° C.-121° C. for 5 min-30 min.
  • It may be prepared into an antibacterial solution, so that it can be used as a preparation for plural subdivided administrations.
  • a sustained release preparation for oral administration once a day is preferable.
  • the water-insoluble material to be used for the film forming agent is, for example, cellulose ethers such as ethylcellulose, butylcellulose and the like; cellulose esters such as cellulose acetate, cellulose propionate and the like; polyvinyl esters such as poly(vinyl acetate), poly(vinyl butyrate) and the like; acrylic polymers such as acrylic acid/methacrylic acid copolymer, methyl methacrylate copolymer, ethoxyethyl methacrylate/cinnamoethyl methacrylate/aminoalkyl methacrylate copolymer, polyacrylic acid, polymethacrylic acid, methacrylic acid alkylamide copolymer, poly(methyl methacrylate), polymethacrylate, polymethacryl amide, aminoalkyl methacrylate copolymer, poly(methacrylic anhydride) and glycidyl methacrylate copolymer, particularly Eudragits
  • a polymer having an acidic dissociable group, which shows pH-dependent swelling is preferable, and a polymer having an acidic dissociable group, which shows less swelling in an acidic range, such as in the stomach, but otherwise in a neutral range, such as in the small intestine and large intestine, is preferable.
  • polymer having an acidic dissociable group which shows pH-dependent swelling
  • crosslinking type polyacrylic acid polymers such as Carbomer 934P, 940, 941, 974P, 980, 1342 and the like, polycarbophil, calcium polycarbophil (all mentioned above are the product of BF Goodrich), HI-BIS-WAKO 103, 104, 105, 304 (all being products of Waco Pure Chemicals Industries, Ltd.) and the like.
  • the film forming agent to be used for the sustained release preparation may further contain a hydrophilic material.
  • hydrophilic material examples include polysaccharides optionally having a sulfuric acid group such as pullulan, dextrin, alkali metal salt of alginic acid and the like; polysaccharides having a hydroxy alkyl group or a carboxy alkyl group such as hydroxypropylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose and the like; methylcellulose, polyvinylpyrrolidone, polyvinyl alcohol, polyethylene glycol and the like.
  • a sulfuric acid group such as pullulan, dextrin, alkali metal salt of alginic acid and the like
  • polysaccharides having a hydroxy alkyl group or a carboxy alkyl group such as hydroxypropylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose and the like
  • methylcellulose polyvinylpyrrolidone
  • polyvinyl alcohol polyethylene glycol and the like.
  • the content of the water-insoluble material of the film forming agent for a sustained release preparation is about 30-about 90% (w/w), preferably about 35-about 80% (w/w), more preferably about 40-75% (w/w), and the content of the swellable polymer is about 3-about 30% (w/w), preferably about 3-about 15% (w/w).
  • the film forming agent may further contain a hydrophilic material, in which case the content of the hydrophilic material for film forming agent is not more than about 50% (w/w), preferably about 5-about 40% (w/w), more preferably about 5-about 35% (w/w).
  • the above-mentioned % (w/w) is a percentage relative to the film forming agent composition wherein the solvent (e.g., water, lower alcohol such as methanol, ethanol and the like) has been removed from the film forming liquid agent.
  • the solvent e.g., water, lower alcohol such as methanol, ethanol and the like
  • a sustained release preparation is produced by preparing a core containing a drug as exemplarily mentioned below, and coating the resulting core with a film forming liquid agent prepared by dissolving by heating or dissolving or dispersing in a solvent a water-insoluble material, a swellable polymer and the like.
  • the form of the core containing a drug (hereinafter sometimes simply referred to as a core) to be coated with a film forming agent is not particularly limited, but it is preferably formed into particles such as granules, fine granules and the like.
  • the average particle size thereof is preferably about 150-2,000 ⁇ m, more preferably about 500-about 1,400 ⁇ m.
  • the core can be prepared by a typical production method.
  • a drug is mixed with suitable excipients, binders, disintegrators, lubricants, stabilizers and the like, and subjected to wet extrusion granulation, fluidized bed granulation and the like.
  • the drug content of the core is about 0.5-about 95% (w/w), preferably about 5.0-about 80% (w/w), more preferably about 30-about 70% (w/w).
  • excipient to be contained in the core examples include saccharides such as sucrose, lactose, mannitol, glucose and the like, starch, crystalline cellulose, calcium phosphate, cornstarch and the like. Of these, crystalline cellulose and corn starch are preferable.
  • binder examples include polyvinyl alcohol, hydroxypropylcellulose, polyethylene glycol, polyvinylpyrrolidone, Pluronic F68, gum arabic, gelatin, starch and the like.
  • disintegrator examples include carboxymethylcellulose calcium (ECG505), crosscarmellose sodium (Ac-Di-Sol), crosslinked polyvinylpyrrolidone (Crospovidone), low substituted hydroxypropylcellulose (L-HPC) and the like. Of these, hydroxypropylcellulose, polyvinylpyrrolidone and low substituted hydroxypropylcellulose are preferable.
  • Examples of the lubricant and coagulation preventive include talc, magnesium stearate and inorganic salts thereof, and examples of the lubricant include polyethylene glycol and the like.
  • examples of the stabilizer include acids such as tartaric acid, citric acid, succinic acid, fumaric acid, maleic acid and the like.
  • the core can be also prepared by, besides the above-mentioned production methods, for example, rolling granulation wherein a drug or a mixture of a drug and an excipient, a lubricant and the like is added by small portions while spraying a binder dissolved in a suitable solvent such as water, lower alcohol (e.g., methanol, ethanol and the like) and the like on an inert carrier particles to be the center of the core, a pan coating method, a fluidized bed coating method or a melt granulating method.
  • a suitable solvent such as water, lower alcohol (e.g., methanol, ethanol and the like) and the like
  • the inert carrier particle include those prepared from sucrose, lactose, starch, crystalline cellulose and waxes, which preferably have an average particle size of about 100 ⁇ m-about 1,500 ⁇ m.
  • the surface of the core may be coated with a protective agent.
  • the protective agent include the aforementioned hydrophilic material, water-insoluble material and the like.
  • the protective agent preferably polyethylene glycol, polysaccharides having a hydroxy alkyl group or a carboxy alkyl group, more preferably hydroxypropylmethylcellulose and hydroxypropylcellulose are used.
  • the protective agent may contain, as a stabilizer, an acid such as tartaric acid, citric acid, succinic acid, fumaric acid, maleic acid and the like, and a lubricant such as talc and the like.
  • the amount to be coated is about 1-about 15% (w/w), preferably about 1-about 10% (w/w), more preferably about 2-about 8% (w/w), relative to the core.
  • the protective agent can be coated by a typical coating method.
  • the protective agent is, for example, spray-coated to the core by a fluidized bed coating method, a pan coating method, and the like.
  • the core obtained in the aforementioned I is coated with a film forming liquid agent prepared by dissolving by heating or dissolving or dispersing in a solvent the aforementioned water-insoluble material, a pH-dependent swellable polymer, and a hydrophilic material to provide a sustained release preparation.
  • a spray coating method and the like can be employed.
  • composition ratio of the water-insoluble material, swellable polymer or hydrophilic material in the film forming liquid agent is suitably determined such that each component of the coating film meets the aforementioned content.
  • the coating amount of the film forming agent is about 1-about 90% (w/w), preferably about 5-about 50% (w/w), more preferably about 5-35% (w/w), relative to the core (exclusive of the coating amount of protective agent).
  • water or organic solvents can be used alone or in a mixture of the both.
  • the mixing ratio (water/organic solvent: weight ratio) of water and the organic solvent in the mixture can vary within the range of 1-100%, which is preferably 1-about 30%.
  • the organic solvent is not subject to any particular limitation as long as it dissolves the water-insoluble material.
  • lower alcohols such as methyl alcohol, ethyl alcohol, isopropyl alcohol, n-butyl alcohol and the like, lower alkanone such as acetone and the like, acetonitrile, chloroform, methylene chloride and the like are used. Of these, lower alcohol is preferable, and ethyl alcohol and isopropyl alcohol are particularly preferable.
  • the film forming liquid agent may contain an acid such as tartaric acid, citric acid, succinic acid, fumaric acid, maleic acid and the like for the stabilization of the film forming liquid agent.
  • the method follows a conventional coating method, which specifically includes spray coating the core with a film forming liquid agent by, for example, a fluidized bed coating method, a pan coating method and the like.
  • a conventional coating method which specifically includes spray coating the core with a film forming liquid agent by, for example, a fluidized bed coating method, a pan coating method and the like.
  • talc, titanium oxide, magnesium stearate, calcium stearate, light anhydrous silicic acid and the like may be added as a lubricants and glycerine fatty acid ester, hydrogenated castor oil, triethyl citrate, cetyl alcohol, stearyl alcohol and the like may be added as a plasticizer.
  • an antistatic agent such as talc and the like may be added as necessary.
  • a rapid release preparation may be a liquid (solution, suspension, emulsion and the like) or a solid (particle, pill, tablet and the like).
  • An agent for oral administration, and an agent for parenteral administration, such as injection and the like, are used, with preference given to an agent for oral administration.
  • a rapid release preparation may generally contain, in addition to the drug, which is an active ingredient, carriers, additives and excipients (hereinafter sometimes simply referred to as excipient) conventionally used in the field of preparation.
  • excipient for a preparation is not subject to any particular limitation as long as it is conventionally employed as an excipient for a preparation.
  • the excipient for the oral solid preparation includes lactose, starch, corn starch, crystalline cellulose (Asahi Kasei Corporation, Avicel PH101 and the like), powder sugar, granulated sugar, mannitol, light anhydrous silicic acid, magnesium carbonate, calcium carbonate, L-cysteine and the like, preferably corn starch and mannitol and the like.
  • excipients may be used alone or in combination.
  • the content of the excipient is, for example, about 4.5-about 99.4 w/w %, preferably about 20-about 98.5 w/w %, more preferably about 30-about 97 w/w %, of the total amount of the rapid release preparation.
  • the drug content of the rapid release preparation is appropriately determined from the range of about 0.5-about 95%, preferably about 1-about 60%, of the total amount of the rapid release preparation.
  • the rapid release preparation When the rapid release preparation is an oral solid preparation, it generally contains a disintegrator in addition to the above-mentioned components.
  • the disintegrator include calcium carboxymethylcellulose (Gotoku Pharmaceutical Co., Ltd., ECG-505), crosscarmellose sodium (e.g., Asahi Kasei Corporation, acjizol), Crospovidone (e.g., colidone CL, BASF), low substituted hydroxypropylcellulose (Shin-Etsu Chemical Co., Ltd.), carboxymethyl starch (Matsutani Chemical Industry Co., Ltd.), sodium carboxymethyl starch (Kimura Sangyo, exprotab), partially a starch (PCS, Asahi Kasei Corporation) and the like.
  • the disintegrator include calcium carboxymethylcellulose (Gotoku Pharmaceutical Co., Ltd., ECG-505), crosscarmellose sodium (e.g., Asahi Kasei Corporation, acjizol), Crospovidone (e.
  • one capable of disintegrating granules by water absorption, swelling, forming a channel between the active ingredient constituting the core and an excipient upon contact with water and the like can be used.
  • These disintegrators can be used alone or in combination.
  • the amount of the disintegrator is appropriately determined depending on the kind of the combination drug to be used and amount thereof, design of the release preparation and the like. It is generally about 0.05-about 30 w/w %, preferably about 0.5-about 15 w/w %, relative to the total amount of the rapid release preparation.
  • the oral solid preparation may further contain, in addition to the above-mentioned composition, typical additives used for solid preparation on demand.
  • the additive include a binder (e.g., sucrose, gelatin, gum arabic powder, methylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, carboxymethylcellulose, polyvinylpyrrolidone, Pullulan, dextrin etc.), a lubricant (e.g., polyethylene glycol, magnesium stearate, talc, light anhydrous silicic acid (e.g., Aerosil (Nippon Aerosil)), a surfactant (e.g., anionic surfactant such as sodium alkylsulfate etc., non-ionic surfactant such as polyoxyethylene fatty acid ester and polyoxyethylenesorbitan fatty acid ester, polyoxyethylene castor oil derivative etc., and the like), a coloring agent (e.g., tar color, caramel, iron oxide, a coloring agent (e.g
  • binder examples include hydroxypropylcellulose, polyethylene glycol, polyvinylpyrrolidone and the like.
  • the rapid release preparation can be prepared based on the conventional preparation method, by mixing each of the aforementioned components, and where necessary, further kneading and forming.
  • the above-mentioned mixing can be performed by a conventional method, such as mixing, kneading and the like.
  • a vertical granulator when a rapid release preparation is formed into particles, a vertical granulator, a universal kneader (HATA Tekkohjo), a fluidized bed granulator FD-5S (Powrex Corporation) and the like are used for mixing, which is followed by granulating by wet extrusion granulation, fluidized bed granulation and the like, to give the preparation, as in the preparation of the core of the aforementioned sustained release preparation.
  • a vertical granulator a universal kneader (HATA Tekkohjo), a fluidized bed granulator FD-5S (Powrex Corporation) and the like are used for mixing, which is followed by granulating by wet extrusion granulation, fluidized bed granulation and the like, to give the preparation, as in the preparation of the core of the aforementioned sustained release preparation.
  • the rapid release preparation and the sustained release preparation thus obtained may be used as they are.
  • suitable separate preparation along with an excipient for a preparation and the like according to a conventional method they may be administered simultaneously or at optional administration intervals.
  • they may be each prepared into a single preparation for oral administration (e.g., granule, fine granule, tablet, capsule and the like) as they are or together with excipient for preparation and the like as appropriate.
  • the both preparations are converted to granules or fine granules and filled in a single capsule and the like to give a preparation for oral administration.
  • the sublingual tablet, buccal preparation and oral cavity rapid disintegrator may be a solid preparation such as tablet and the like or an oral cavity mucous membrane adhesion tablet (film).
  • a preparation containing a combination drug and an excipient is preferable. It may contain auxiliaries such as a lubricant, an isotonic agent, a hydrophilic carrier, a water dispersible polymer, a stabilizer and the like.
  • auxiliaries such as a lubricant, an isotonic agent, a hydrophilic carrier, a water dispersible polymer, a stabilizer and the like.
  • ⁇ -cyclodextrin or ⁇ -cyclodextrin derivative e.g., hydroxypropyl- ⁇ -cyclodextrin and the like
  • Examples of the above-mentioned excipient include lactose, sucrose, D-mannitol, starch, crystalline cellulose, light anhydrous silicic acid and the like.
  • Examples of the lubricant include magnesium stearate, calcium stearate, talc, colloidal silica and the like, particularly magnesium stearate and colloidal silica are preferable.
  • Examples of the isotonicity agent include sodium chloride, glucose, fructose, mannitol, sorbitol, lactose, saccharose, glycerine, urea and the like, particularly mannitol is preferable.
  • hydrophilic carrier examples include swellable hydrophilic carriers such as crystalline cellulose, ethylcellulose, crosslinked polyvinylpyrrolidone, light anhydrous silicic acid, silicic acid, dicalcium phosphate, calcium carbonate and the like, particularly crystalline cellulose (e.g., microcrystalline cellulose and the like) is preferable.
  • swellable hydrophilic carriers such as crystalline cellulose, ethylcellulose, crosslinked polyvinylpyrrolidone, light anhydrous silicic acid, silicic acid, dicalcium phosphate, calcium carbonate and the like, particularly crystalline cellulose (e.g., microcrystalline cellulose and the like) is preferable.
  • water dispersible polymer examples include gum (e.g., gum tragacanth, acacia gum, guar gum), alginate (e.g., sodium alginate), cellulose derivative (e.g., methylcellulose, carboxymethylcellulose, hydroxymethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose), gelatin, soluble starch, polyacrylic acid (e.g., carbomer), polymethacrylic acid, polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, polycarbofil, ascorbic palmitate and the like, with preference given to hydroxypropylmethylcellulose, polyacrylic acid, alginate, gelatin, carboxymethylcellulose, polyvinylpyrrolidone, polyethylene glycol and the like.
  • gum e.g., gum tragacanth, acacia gum, guar gum
  • alginate e.g., sodium alginate
  • cellulose derivative e.g., methylcellulose, carb
  • hydroxypropylmethylcellulose is preferable.
  • the stabilizer include cysteine, thiosorbitol, tartaric acid, citric acid, sodium carbonate, ascorbic acid, glycine, sodium sulfite and the like, particularly, citric acid and ascorbic acid are preferable.
  • the sublingual tablet, buccal and oral cavity rapid disintegrator can be produced by mixing a combination drug and an excipient by a method know per se.
  • the above-mentioned auxiliaries such as a lubricant, an isotonic agent, a hydrophilic carrier, a water dispersible polymer, a stabilizer, a coloring agent, a sweetener, an antiseptic and the like may be contained.
  • the mixture is compression formed under pressure to give sublingual tablet, buccal or oral cavity rapid disintegrator.
  • a solvent such as water, alcohol and the like is used to moisten or wet as necessary before and after the compression forming. After the forming, the tablets may be dried.
  • a combination drug and the above-mentioned water dispersible polymer preferably, hydroxypropylcellulose, hydroxypropylmethylcellulose
  • an excipient and the like are dissolved in a solvent such as water and the like, and the obtained solution is cast to give a film.
  • an additive such as a plasticizer, a stabilizer, an antioxidant, a preservative, a coloring agent, a buffer, a sweetener and the like may be added.
  • glycols such as polyethylene glycol, propylene glycol and the like may be added, and to increase adhesion of the film to the oral cavity mucous membrane lining, bioadhesive polymer (e.g., polycarbofil, carbopol) may be added.
  • the casting includes pouring the solution on a non-adhesive surface, spreading the solution in a uniform thickness (preferably about 10-1000 ⁇ ) with a coating tool such as doctor blade and the like and drying the solution to give a film.
  • the film thus formed may be dried at room temperature or under heating and cut into a desired surface area.
  • Examples of preferable oral cavity rapid disintegrator are a solid rapid diffusing administration agent having a net structure of a combination drug and water soluble or water diffusable carrier which are inert to a combination drug.
  • the net structure can be obtained by sublimation of a solvent from the solid composition consisting of a solution obtained by dissolving a combination drug in a suitable solvent.
  • the oral cavity rapid disintegrator preferably contains, in addition to a combination drug, a matrix forming agent and a secondary component.
  • the matrix forming agent examples include animal proteins or vegetable proteins such as gelatins, dextrins, soybeans, wheat, psyllium seed protein and the like; rubber substances such as gum arabic, guar gum, agar, xanthan and the like; polysaccharides; alginic acids; carboxymethylcelluloses; carrageenans; dextrans; pectins; synthetic polymers such as polyvinylpyrrolidone and the like; a material derived from a gelatin-gum arabic complex and the like.
  • animal proteins or vegetable proteins such as gelatins, dextrins, soybeans, wheat, psyllium seed protein and the like
  • rubber substances such as gum arabic, guar gum, agar, xanthan and the like
  • polysaccharides alginic acids
  • carboxymethylcelluloses carboxymethylcelluloses
  • carrageenans dextrans
  • pectins synthetic polymers
  • synthetic polymers such as polyvinylpyrrolidone and
  • saccharides such as mannitol, dextrose, lactose, galactose, trehalose and the like; cyclic saccharides such as cyclodextrin and the like; inorganic salts such as sodium phosphate, sodium chloride, aluminum silicate and the like; amino acid having 2 to 12 carbon atoms such as glycine, L-alanine, L-aspartic acid, L-glutamine acid, L-hydroxyproline, L-isoleucine, L-leucine, L-phenylalanine and the like are exemplified.
  • matrix forming agents may exist with a surfactant or without a surfactant.
  • the matrix forming agent can form a matrix, and also can help maintain the diffusion of a combination drug in the solution or suspension.
  • the composition may contain a secondary component such as a preservative, an antioxidant, a surfactant, a thickener, a coloring agent, a pH adjusting agent, a flavor, a sweetener, a taste masking reagent and the like.
  • a suitable coloring agent include red, black and yellow ferric oxides and FD&C dyes of Ellis & Everard, such as FD&C blue NO. 2, FD&C red No. 40 and the like.
  • a suitable flavor contains mint, raspberry, licorice, orange, lemon, grapefruit, caramel, vanilla, cherry, grape flavor and a combination of these.
  • Suitable pH adjusting agent includes citric acid, tartaric acid, phosphoric acid, hydrochloric acid and maleic acid.
  • Suitable sweetener includes aspartame, acesulfame K, thaumatin and the like.
  • Suitable taste masking agent includes sodium bicarbonate, ion exchange resin, cyclodextrin inclusion compound, adsorbent substance and microcapsuled apomorphine.
  • one containing a combination drug generally in a proportion of about 0.1-about 50 wt %, preferably about 0.1-about 30 wt %, which is capable of dissolving 90% or more of a combination drug in water for about 1 min-about 60 min, preferably about 1 min-about 15 min, more preferably about 2 min-about 5 min, such as the above-mentioned sublingual tablet, buccal and the like, and an oral cavity rapid disintegrator that disintegrates within 1-60 sec, preferably 1-30 sec, more preferably 1-10 sec, after being placed in an oral cavity, are preferable.
  • the content of the above-mentioned excipient in the whole preparation is about 10-about 99 wt %, preferably about 30-about 90 wt %.
  • the content of the ⁇ -cyclodextrin or ⁇ -cyclodextrin derivative relative to the whole preparation is 0-about 30 wt %.
  • the content of the lubricant relative to the whole preparation is about 0.01-about 10 wt %, preferably about 1-about 5 wt %.
  • the content of the isotonicity agent relative to the whole preparation is about 0.1-about 90 wt %, preferably about 10-about 70 wt %.
  • the content of the hydrophilic carrier relative to the whole preparation is about 0.1-about 50 wt %, preferably about 10-about 30 wt %.
  • the content of the water dispersible polymer relative to the whole preparation is about 0.1-about 30 wt %, preferably about 10-about 25 wt %.
  • the content of the stabilizer relative to the whole preparation is about 0.1-about 10 wt %, preferably about 1-about 5 wt %.
  • the above-mentioned preparation may contain additives such as a coloring agent, a sweetener, an antiseptic and the like as necessary.
  • the respective amounts of the combination drug may be, for example, about 0.01 to about 1000 mg/kg, preferably about 0.01 to about 100 mg/kg, more preferably about 0.1 to about 100 mg/kg, most preferably about 0.1 to about 50 mg/kg, and particularly about 1.5 to about 30 mg/kg per day, for a patient (adult weighing about 60 kg) with, for example, prostate cancer, said daily dose being given intravenously once or in several portions during a day. It is a matter of course that a lower daily dose may be sufficient or an excessive dose may be required since the dose may vary depending on various factors as discussed above.
  • the combination drug may be contained in any amount as long as a side effect does not pose a problem. While the daily dose of the combination drug may vary depending on the disease state, age, sex, body weight and difference in sensitivity of the administration object, timing and interval of administration, characteristics, dispensing and kind of the pharmaceutical preparation, the kind of the active ingredient and the like, and is not particularly limited, the amount of the drug is generally about 0.001-2000 mg, preferably about 0.01-500 mg, more preferably about 0.1-100 mg, per 1 kg body weight of mammal by oral administration, which is generally administered once or in 2 to 4 portions during a day.
  • the preparation comprising a combination drug When the preparation comprising a combination drug is administered, it may be administered at the same time. However, a combination drug may be administered first, and then the LHRH receptor agonist or antagonist and an androgen receptor agonist may be administered. Alternatively, the LHRH receptor agonist or antagonist and an androgen receptor agonist may be administered first, and then a combination drug may be administered. For time-staggered administration, the time difference varies depending on the active ingredient to be administered, dosage form and administration route. For example, when the combination drug is to be administered first, the LHRH receptor agonist or antagonist and an androgen receptor agonist are administered within 1 min-3 days, preferably 10 min-1 day, more preferably 15 min-1 hour, after the administration of the combination drug.
  • the combination drug is administered within 1 min-1 day, preferably 10 min-6 hrs., more preferably 15 min-1 hour, after the administration of the LHRH receptor agonist or antagonist and an androgen receptor agonist.
  • Leuprorelin acetate (5.8 g) was dissolved in 6.7 ml of distilled water. Thereto was added dichloromethane solution (138 g) containing polylactic acid (weight average molecular weight: 15000)(51.6 g) separately dissolved and filtered, and emulsified with stirring (rotation: about 6000 rpm) in an autominimixer for 9 min. and adjusted to 15° C. This was added to 0.1% aqueous polyvinyl alcohol (PVA) solution (13.5 L) dissolved, filtered and adjusted to the same temperature in advance and emulsified.
  • PVA polyvinyl alcohol
  • HOMOMIC LINE FLOW TOKUSHU KIKA KOGYO CO., LTD.
  • TOKUSHU KIKA KOGYO CO., LTD. TOKUSHU KIKA KOGYO CO., LTD.
  • This W/O/W emulsion was desolvented with gently stirring for about 3 hrs. (an in-water drying method).
  • microcapsules were passed through a 74 ⁇ m sieve to remove coarse particles, separated by filtration or centrifugal separation. This was washed with distilled water, and after removing a free drug and PVA and redispersed in a small amount of water.
  • D mannitol (8.7 g) was dissolved and the mixture was passed through a sieve and lyophilized. The shelf temperature during drying was gradually raised and finally dried at 52° C. for 69 hrs. This was pulverized by passing through a sieve to give a microcapsule powder. This operation gave 58 g of 15% D-mannitol-containing microcapsule powder.
  • Testosterone (5.0 mg) and sodium chloride (20.0 mg) are dissolved in distilled water and water is added to make the total amount 2.0 ml.
  • the solution is filtered and filled in a 2 ml ampoule under sterile conditions.
  • the ampoule is sterilized and sealed to give a solution for injection.
  • the aforementioned (1)-(6) were mixed and tableted by a tableting machine to give tablets.
  • LNCaP-FGC and MDA PCa 2b cell lines were cultured in a culture medium free of androgen (RPMI1640+10% Dextran Charcoal (DCC)-Fetal Bovine Serum (FBS) for LNCaP-FGC and Ham's F-12K+25 ng/ml cholera toxin+10 ng/ml EGF+0.005 mM phosphoethanolamine+100 pg/ml hydrocortisone+45 nM selenious acid+0.005 mg/ml insulin+20% DCC-FBS for MDA PCa 2b).
  • the lines did not grow at first but started to grow after continued culture for 3 to 8 months or longer.
  • the cells thereof were named LNCaP-hr and MDA PCa 2b-hr, respectively.
  • LNCaP-hr (cultured for 60 weeks in culture broth free of androgen) and LNCaP-FGC cells were plated on a 24 well plate at 40000 cells/mL/well, 0.01 to 10 ng/mL of testosterone was added the next day, and the cells were counted 3 days after the addition.
  • MDA PCa 2b-hr (cultured for 61 weeks in culture broth free of androgen) and MDA PCa 2b cells were plated on a 24 well plate at 40000 cells/mL/well, 0.1 to 100 ng/mL of testosterone was added the next day, and the cells were counted 4 days after the addition.

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US10/507,044 2002-03-11 2003-03-10 Remedies for sex hormone-dependent disease Abandoned US20050159361A1 (en)

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US20080032935A1 (en) * 2006-08-07 2008-02-07 Aeterna Zentaris Gmbh Application of initial doses of lhrh analogues and maintenance doses of lhrh antagonists for the treatment of hormone-dependent cancers and corresponding pharmaceutical kits
US20110312925A1 (en) * 2010-06-16 2011-12-22 Endorecherche, Inc. Methods of treating or preventing estrogen-related diseases
US11306146B2 (en) 2014-09-17 2022-04-19 Merck Patent Gmbh Method of treating bone metastasis diseases, medicaments therefore, and a method of predicting the clinical outcome of treating bone metastasis diseases
US11415581B2 (en) 2014-09-17 2022-08-16 Merck Patent Gmbh Method of treating solid cancers and/or metastases thereof with pan AV integrin inhibitor, medicaments therefore, and a method of predicting the clinical outcome of treating solid cancers and/or metastases thereof

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US20050159361A1 (en) * 2002-03-11 2005-07-21 Takahito Hara Remedies for sex hormone-dependent disease
KR20050044902A (ko) 2002-08-12 2005-05-13 다케다 야쿠힌 고교 가부시키가이샤 축합 벤젠 유도체 및 용도
KR101074462B1 (ko) 2003-01-29 2011-10-18 다케다 야쿠힌 고교 가부시키가이샤 티에노피리미딘 화합물 및 그 용도
US20060204588A1 (en) * 2005-03-10 2006-09-14 Elan Pharma International Limited Formulations of a nanoparticulate finasteride, dutasteride or tamsulosin hydrochloride, and mixtures thereof
US20080051375A1 (en) * 2006-08-25 2008-02-28 Auerbach Alan H Methods for treating cancer comprising the administration of a vitamin d compound and an additional therapeutic agent, and compositions containing the same
US20080051380A1 (en) 2006-08-25 2008-02-28 Auerbach Alan H Methods and compositions for treating cancer
US20090124587A1 (en) * 2007-07-12 2009-05-14 Auerbach Alan H METHODS FOR TREATING CANCER USING 17alpha-HYDROXYLASE/C17,20-LYASE INHIBITORS
BR112017021044A2 (pt) * 2015-04-03 2018-07-24 Santen Pharmaceutical Co., Ltd. agente terapêutico para o olho seco compreendendo nandrolona ou éster da mesma ou metenolona ou éster da mesma como um ingrediente ativo
CA3031705A1 (fr) 2016-07-29 2018-02-01 Janssen Pharmaceutica Nv Traitement du cancer de la prostate a l'aide de niraparib
US11977085B1 (en) 2023-09-05 2024-05-07 Elan Ehrlich Date rape drug detection device and method of using same

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US6113943A (en) * 1996-10-31 2000-09-05 Takeda Chemical Industries, Ltd. Sustained-release preparation capable of releasing a physiologically active substance
US5981550A (en) * 1997-06-05 1999-11-09 Merck & Co., Inc. Antagonists of gonadotropin releasing hormone
US6077847A (en) * 1998-04-02 2000-06-20 Merck & Co., Inc. Antagonists of gonadotropin releasing hormone

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080032935A1 (en) * 2006-08-07 2008-02-07 Aeterna Zentaris Gmbh Application of initial doses of lhrh analogues and maintenance doses of lhrh antagonists for the treatment of hormone-dependent cancers and corresponding pharmaceutical kits
US8921318B2 (en) 2006-08-07 2014-12-30 Aeterna Zentaris Gmbh Application of initial doses of LHRH analogues and maintenance doses of LHRH antagonists for the treatment of hormone-dependent cancers and corresponding pharmaceutical kits
US20110312925A1 (en) * 2010-06-16 2011-12-22 Endorecherche, Inc. Methods of treating or preventing estrogen-related diseases
US11576891B2 (en) * 2010-06-16 2023-02-14 Endorecherche, Inc. Methods of treating or preventing estrogen-related diseases
US11306146B2 (en) 2014-09-17 2022-04-19 Merck Patent Gmbh Method of treating bone metastasis diseases, medicaments therefore, and a method of predicting the clinical outcome of treating bone metastasis diseases
US11415581B2 (en) 2014-09-17 2022-08-16 Merck Patent Gmbh Method of treating solid cancers and/or metastases thereof with pan AV integrin inhibitor, medicaments therefore, and a method of predicting the clinical outcome of treating solid cancers and/or metastases thereof
US11851492B2 (en) 2014-09-17 2023-12-26 Merck Patent Gmbh Method of treating bone metastasis diseases, medicaments therefore, and a method of predicting the clinical outcome of treating bone metastasis diseases

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AU2003211877A1 (en) 2003-09-22
US20080138426A1 (en) 2008-06-12
CA2478827A1 (fr) 2003-09-18
WO2003075958A1 (fr) 2003-09-18
US8518890B2 (en) 2013-08-27
EP1488807A4 (fr) 2009-07-08

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