US20050157386A1 - Tissue specimen holder - Google Patents

Tissue specimen holder Download PDF

Info

Publication number
US20050157386A1
US20050157386A1 US11020387 US2038704A US2005157386A1 US 20050157386 A1 US20050157386 A1 US 20050157386A1 US 11020387 US11020387 US 11020387 US 2038704 A US2038704 A US 2038704A US 2005157386 A1 US2005157386 A1 US 2005157386A1
Authority
US
Grant status
Application
Patent type
Prior art keywords
tissue
tray
index
specimen
imaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
US11020387
Other versions
US9128024B2 (en )
Inventor
Roger Greenwald
James Zavislan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliber Imaging & Diagnostics Inc
Zavislan James M
Original Assignee
Lucid Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/0303Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0028Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/34Microscope slides, e.g. mounting specimens on microscope slides
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/043Hinged closures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0609Holders integrated in container to position an object
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0654Lenses; Optical fibres
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0672Integrated piercing tool
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0822Slides
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0677Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N2021/0339Holders for solids, powders
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N2021/0342Solid sample being immersed, e.g. equiindex fluid
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N2021/0364Cuvette constructions flexible, compressible

Abstract

A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system, especially a laser scanning confocal microscope system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. A stabilizing plate is attached to a surface of the bag which faces the window. An objective lens of the confocal imaging system receives and supports the plate, preferably by a magnetic coupling ring around the plate which is magnetically attached to the barrel of the objective lens. The lens may be fixed and a mechanism for positioning the tray with respect to the lens in a direction along the optical axis of the lens and in orthogonal directions perpendicular to that axis shares a common support with the lens. Wavefront distortion which may result from an effectively corrugated surface of the specimen is minimized by selecting an immersion liquid which equals the refractive index of the near surface tissues. The tissue determines the index of the immersion fluid. The thicknesses and refractive indices of the window and plate are then determined to correct the residual aberration of the optical system. Different trays having different window and plate thicknesses and indices may be provided for different specimen species such as kidney, liver, cervix, et cetera. Thus, a generic confocal imaging system with a generic objective lens is corrected for image aberration and wavefront distortion due to the surface corrugations of the tissue. The correction is simplified since the bag is compliant and compresses so that the optical thickness along the axis between the objective lens and the focal plane in or at the specimen is substantially invariant whether the beam is focused at the interface between the specimen and the window or within the specimen over a focusing range which can extend over several millimeters.

Description

  • [0001]
    This application claims the priority benefit of U.S. Provisional Application No. 60/120,470, filed Feb. 17, 1999, which is herein incorporated by reference.
  • [0002]
    The present invention relates to a tissue specimen holder or tray for use in microscopy and particularly in laser scanning confocal microscopy for imaging sections of surgically excised specimens. The invention is especially suitable for providing a tissue tray which aids in the imaging of a wide variety of tissue samples which may be excised tissues or biopsies of various tissues, such as liver, kidney, cervix, et cetera.
  • [0003]
    It has been proposed to provide for the imaging of specimens in an immersion liquid which matches the index of refraction of the tissue (See U.S. Pat. No. 5,719,700, issued Feb. 17, 1998 to P. Corcuff, et al and U.S. Pat. No. 4,208,101, issued Jun. 17, 1980 to L. Trapp, et al). Handling of the tissue specimens and the immersion liquid is difficult. The specimens are small and the liquid can run over a slide on which the specimen is mounted. In short, handling and preparation of specimens for imaging is, with the proposed systems, a messy operation. In addition, variations in optical path between the section of the specimen of interest and the imaging system can distort the image. Such distortions are exacerbated by the corrugated surface provided at the surface of the specimen. For high fidelity imaging, the immersion fluid must closely match the tissue index. Matching the refractive index of the immersion fluid to the tissue greatly reduces the optical refracting power of tissue and therefore the optical path difference introduced by the tissue.
  • [0004]
    It is a feature of the invention to provide specimen holders or tissue trays optimized for different tissues which are usable with the same imaging system, and without replacement or adjustment of objective lenses. The tray provided by the invention may be used to contain the specimen for short term or long term storage or to transport the tissue for additional processing. The tray may be disposable after use. The tray contains and also has associated therewith index matching liquids. The tray facilitates the use of such liquids without messy operations and without adversely affecting imaging (imparting distortion to the image because of the presence of the index matching liquids).
  • [0005]
    The confocal laser scanning microscope imaging systems having objective lenses which are capable of forming images of different sections having different orientations within a specimen may be of the type described in allowed U.S. patent application Ser. No. 08/683,607 filed Jul. 15, 1996 in the name of R. Rox Anderson et al., now U.S. Pat. No. 5,880,880, issued Mar. 9, 1999, and U.S. Pat. No. 5,788,639, filed in the names of James Zavislan and Jay Eastman and issued Aug. 4, 1998.
  • [0006]
    Briefly described, a specimen holder in accordance with the invention includes a container having a window through which an optical beam passes into a specimen disposed in the container over the window. The container may be in the form of a specimen tray. The specimen is preferably held in place by clamps which may be automatically applied when a cover of the container is closed. In use, the container includes an immersion liquid which closely matches the index of the tissue. Matching may be to the average index at the surface of the tissue which interfaces with the window. The tissue surface may be corrugated due to natural or surgically produced surface texture. Such corrugation may alter the wavefront of the beam (make the wavefront depart from a section of a sphere) which enters the tissue and is focused in the tissue section of interest. The distortion due to such variations is reduced in accordance with the invention by virtue of minimizing the variation of optical path lengths, notwithstanding that the section at which the beam is focused may be at the surface of the tissue or within the tissue (for example a distance of up to approximately 3 mm from the surface) by use of immersion liquids and coupling liquids and other transmissive elements, including the window, through which the imaging beam passes. The coupling medium is contained in a bag outside the container and facing the window. The bag is made of compliant transmissive material such as a polymer, for example polyethylene, but is of a minimum thickness so as not to have any material effect on the optical path through the bag. The bag contains a liquid coupling medium. Mounted on the bag is a stabilizing plate of transmissive material. The plate is opposed to the window, and preferably contains a lock-in unit such as a magnet or magnetic ring which is received in a notch at the top of a barrel containing the objective lens of the imaging system. The objective lens and the tray is supported on a fixture which is mounted on a common structure with the objective lens via a translation mechanism, which moves the fixture and the container in nominally orthogonal directions, one of which is along the optical axis of the lens. The index of the coupling medium is preferably the same as the index of the immersion liquid. The tray may have a passageway which is pierced by a protrusion on the cover of the tray so as to allow the coupling liquid to fill the tray and provide the immersion liquid. The stabilizing coupling of the plate to the objective lens prevents tilting and maintains the plate perpendicular to the optical axis notwithstanding of motion of the container so as to bring sections of the specimen of interest into focus and to scan the specimen in the plane of the section. In order to minimize optical distortion, due to changes in optical path between the exit pupil of the objective lens and the section of the specimen being imaged, in spite of the corrugations caused by the surface, the difference between the index of refraction of the immersion liquid and the average index of refraction of the tissue at it's surface, multiplied by the height between the peaks of the hills and bottoms of the valleys of the corrugations, is selected to be equal or less than a quarter wavelength at the wavelength of the laser beam used for imaging. Since the average index is a function of tissue type and clinical condition of the patient, the index matching fluids can be selected in the preparation of the tray for the particular tissue to be imaged. In addition, the thickness and index of the window and the plate may be varied depending upon the type of tissue in the tray in order to reduce spherical aberration for the objective lens used in the imaging system.
  • [0007]
    The foregoing and other features, objects and advantages of the invention will become more apparent from a reading of the following description taken in connection with the accompanying drawings in which:
  • [0008]
    FIG. 1 is a diagram schematically showing the imaging system, its objective lens and a container providing a tray with the tray in one of two alternative positions, where the section is near the surface of the tissue sample, while FIG. 1B shows the position where the section is within the tissue sample (specimen);
  • [0009]
    FIG. 1A is an enlarged view of the section shown within the dashed lines labeled 1A-1A in FIG. 1;
  • [0010]
    FIG. 1B shows the objective lens and tray where the beam is focused at an image plane within the specimen, where the section being imaged is located;
  • [0011]
    FIG. 2 is a diagram illustrating the fixturing for the tray and the mechanism for adjusting the position of the tray in three orthogonal directions, X, Y, and Z;
  • [0012]
    FIG. 3 is a diagram showing the tray in process of preparation;
  • [0013]
    FIG. 4 is an enlarged view illustrating one of the clamp mechanisms used in the tray;
  • [0014]
    FIG. 5 is a diagram illustrating the tray where the cover is in process of being closed;
  • [0015]
    FIG. 6 is a view similar to FIG. 5 showing the tray after closure of the tray;
  • [0016]
    FIG. 7 is a sectional top view taken along the line 7-7 in FIG. 6;
  • [0017]
    FIG. 8 is an enlarged view schematically illustrating the corrugations formed by surface texture at the surface of the tray.
  • [0018]
    Referring more particularly to FIGS. 1, 1A, 1B, 2 and 7, there is shown a tissue holder or tray 10 mounted on a support platform 12. The support platform 12 is moveable by a translation mechanism 14 which is mounted on a stand 16 which holds an objective lens assembly 18 in a nominally fixed position. The platform 12, translation mechanism 14 and stand 16 provide a tray support fixture which can receive various trays. The lens assembly 18 includes a lens barrel 20 and a lens 22 mounted within the barrel. The upper end of the barrel has an annular notch 24 in which a portion of the tray 10 is received and locked, as will be discussed in greater detail below.
  • [0019]
    The tissue tray or holder 10 is an assembly having a generally open specimen container or box with a plate providing a base 26, sidewalls 28 and a cover 30, which may be hinged to one of the side walls. The shape of the box is shown as rectangular but it may be circular or oblong. The base has an opening containing a window 32 of transparent material. The thickness and refractive index of this window is selected to accommodate the design of the lens 18 and the index of refraction of the tissue specimen 34. The specimen may be surgically excised.
  • [0020]
    A compliant bag 44 of thin, optically transparent material, is attached to the underside of the base 26 of the tray 10. This bag is initially filled with an optical coupling medium which may also provides an immersion medium for the specimen 34. To prepare the tray, the specimen 34 is placed on the window 32 as shown in FIG. 3. Clamping mechanisms 40 are then used to hold down the tissue specimen on the window for viewing, as shown in FIG. 5. The mechanisms are shown engaging the specimen 34 in FIG. 5, but may automatically engage the specimen when the cover is closed, as shown in connection with FIG. 4. When the cover is closed, a pin 50, carried on the cover, pierces a plug 45 in an opening 46 which provides a passageway for the flow of the coupling medium into the tray through the pierced opening 46. When the tray 10 is placed on the support platform, a transparent stabilizing plate 52 which is attached to the bag 44 in a location near the bottom of the bag opposite to the window 32, is captured in the notch 24 at the upper end of the lens barrel. The relative heights of the platform 12 and the lens barrel 24, both of which are supported in the fixture 16, is such that the bag 44 is compressed by the lens barrel and the shape thereof changes from the shape shown in FIG. 5 to the shape shown in FIG. 6. Then the liquid optical coupling medium 39 flows through the hole 46 and encompasses the specimen. The coupling liquid then serves as the immersion liquid. In another embodiment of the invention, the tray may be filled with the immersion liquid, and, when the plug is pierced, the immersion liquid flows into the bag. In a further embodiment, the bag and tray are independently filled. When plug 45 is opened the fluids can mix. In a further embodiment the bag and tray are independently filled. There is no plug 45 and the liquids remain separate.
  • [0021]
    In either case, the height of the liquid above the lens and above the base depends upon the relative position of the tray and may vary as the tray is moved to select the focus in the section of the specimen to be imaged. See FIG. 1B. The configuration of the bag also changes as the tray moves with respect to the objective lens 18 to scan the section.
  • [0022]
    The objective lens 18 may be a generic lens which is corrected for spherical aberrations for a cover slip of certain index NT and thickness T. The spherical aberration present in the objective lens is equal and opposite the spherical aberration introduced by the cover media or slip. When the focus is adjusted to the top surface of window 32, the cover medium includes plate 52, coupling medium 39, and window 32. The spherical aberration can be described by several representations such as longitudinal ray aberration, transverse ray aberrations or wavefront aberration. Using the longitudinal ray aberration, the spherical aberration=LAT. The longitudinal aberration can be minimized by a single plate of index NT and thickness T or a series of plates such that where the plate is in air, LA T ( N T , T ) = i = 1 i = N LA T ( N i , t i ) , where , LA ( N i , t i ) = t i Ni [ 1 - N i Cos U N i 2 - sin 2 U ]
    and where U is the angle in air with respect to the optical axis of the marginal ray in the converging beam. Ni and ti is the index and thickness of each of the plates. The relationships when the plate is liquid are similar. See Warren Smith, Optical Engineering, pages 96-99 published by McGraw Hill, (1990) for further information on the equations given above.
  • [0023]
    Aberration is introduced by the specimen and particularly by the surface texture of the specimen in the optical path (along the axis of the lens 18). A laser beam from a confocal imaging system 36 passes through the lens along the optical path and is focused in the specimen. The tissue defines a corrugated surface as shown in FIG. 8. There is index variation between the tissue surface and the window 32, which may be accommodated in part, by a selection of the index and thickness in the direction of the beam (along the optical axis) through the window 32. The other indices of refraction of the elements in the beam path are also taken into account in determining the thickness and index of either the window 32, plate 52, or both. The primary determinative of the index and thickness of the window 32 is the index of the tissue of the specimen 34. Thus, the index and thickness of the window 32 (or plate 52) will depend upon the type of specimen being imaged. Different trays 10 are provided for different types of specimens (kidney, liver, cervix, et cetera) and each will have a somewhat different window thickness and index in order to reduce spherical aberration.
  • [0024]
    An immersion liquid 38 having an index which generally matches the average index of the tissue of the specimen 34 is contained in the tray. This liquid may serve also as a tissue preservative or fixative.
  • [0025]
    When the tissue specimen 34 is placed in the tray in the base 26 and over the window 32 as shown in FIGS. 3 and 4, the specimen 34 is held down by the clamp mechanism 40. The clamp mechanism which is illustrated has hooked or barbed fingers which are hinged to the sidewalls 28 at spaced locations. The mechanisms include springs 42 which provide over center locks, such that when the fingers are pressed down beyond their axis of rotation, they are held down by the springs 42. Other clamping mechanisms may be used such as meshes or a membrane overlay or a permeable or perforated bag. Fiducial marks, which can be visualized or imaged, may be provided in the case of meshes or membranes. The use of a membrane or mesh may be preferable since the specimen 34 may be moved under the membrane. The membrane specimen tray or cassette is the subject matter of a companion application filed concurrently herewith in the name of Bill Fox, et al. Further information as to the use of the markings on the clamping mechanism (the mesh or membrane) to mark locations of the image tissue is contained in a co-pending International Patent Application No. PCT/US99/21116, and U.S. patent application, filed in the names of Roger J. Greenwald and James M. Zavislan, Ser. No. 60/100,176, filed Sep. 14, 1998. The purpose of the clamps is to keep the tissue stationary during examination and also provide a means to lightly compress the tissue surface against the window. Alternatively, the clamps may provide tension to pull the tissue surface taut. Holding the tissue with either compression normal to the window or in tension parallel to the window (or both) tends to reduce the surface texture, or corrugation, peak to valley depth.
  • [0026]
    As shown in FIG. 1, the thin compliant bag 44 is attached to the base 26 and encompasses the window 32 and the initially plugged opening 46. The bag is filled with the optical coupling medium 39, which may have an index selected in order to reduce image distortion due to the corrugations formed by the texture surface of the specimen 34 via which the optical imaging beam passes. Preferably the coupling liquid is the same as the immersion liquid 38 and the coupling liquid may flow through the opening 46 which may initially have a plug 45 and be plugged and then opened by the pin 50 carried by the cover 30, when the cover is closed as shown in FIG. 6.
  • [0027]
    The stabilizing plate 52 is attached to the bag 44 opposite to the window 32. This plate has an index of refraction and thickness which is taken into account in selecting the thickness and index of the window 32. A magnet or magnetic ring 56 surrounds the plate 52. See FIG. 1A. The plate 52 may be held by fusing, welding, cementing, friction or screw fit into the ring 56. The ring is permanently attached, as by a fused or cemented connection, to the bag 44, or to an opening in the bag. The diameter of the ring 52 is sized to fit into a notch 58 at the upper end of the barrel of the objective lens. The barrel may be made of magnetic material so as to lock the bag 44 in place on the assembly of the objective lens 18. The attachment is removable since the hold down force is magnetic. Other removable or releasable lock in mechanisms, such as snaps, may be used. The plate 52 may be curved or of a meniscus shape to provide optical power added to the power of the objective, if desired.
  • [0028]
    The confocal imaging system 36 may be of the type described in the above referenced Anderson and Zavislan patents. Imaging systems using two-photon microscopy or optical coherence tomography may also be used. See Denk et al., U.S. Pat. No. 5,034,613 and Schmitt et al., Proc. SPIE, volume 1889 (1993). Associated with the imaging system is a monitor or display 60 which provides a display of the image of the section. The image may also be stored digitally in memory shown as image storage 62. The location of the section being imaged is obtained by user controls 64 which may provide signals for actuating drive motors or other actuators in the translation mechanism 14 which selects the section to be imaged and can scan the section. Alternatively, the translator stage may be manually controlled by the use of micrometers. The image storage 62 or the imaging system may be connected through a switch 66 to a telepathology transmission system 68 which transmits the image to a remote location. Such a system 68 is the subject of U.S. Pat. No. 5,836,877 issued Nov. 17, 1988 to J. Zavislan.
  • [0029]
    As shown in FIGS. 1 and 1B, the lens 18 may focus the beam at the surface or within the specimen by moving the support 12 thereby varying the distance between the lens and the tray. As the distance along the optical axis to that inside the tissue changes, the thickness along that axis of the coupling and medium 39 varies in an opposite sense. This keeps the optical path constant, even though the section may be well within the specimen. This inverse relationship of bag thickness to focus depth is in a direction to compensate for aberration due to physical length variations between the lens and the section of the medium being imaged.
  • [0030]
    As shown in FIG. 8, the corrugations due to the surface texture of the specimen 34 creates corrugations having a depth (h) (from the apex of the corrugation peaks to the bottom of the valleys of the corrugations) which may be approximately 200 microns in length. The index of refraction of the tissue is nT, while the index of refraction of the immersion fluid, which fills the corrugations providing the surface texture of the specimen 34, have an index nI. The beam is focused at a focus f in the section to be imaged. The wavefront which may be spherical, can be distorted due to an optical path difference φ imprinted on the wavefront which converges to the focus F. This path difference is a function of the product of the corrugation height h and the difference between nT and nI. The use of the index matching fluid reduces the optical path difference so that the imprint is minimized. The optical path difference φ is shown enlarged at 63 in FIG. 8. This optical path difference may also be viewed as the wavefront which is propagating to the focus F. This wavefront may be spherical and part of a sphere as shown at 68 prior to passing through corrugations at the surface of the specimen 34. The optical path distortion after transmission through the tissue surface is approximated by the relation
    φ=h(n T −n I)
    where h is the mechanical depth of surface texture. In order to correct for the distortion of the beam wavefront (which may be a spherical wavefront) by virtue of the variation in index of refraction presented by corrugations, it is desirable that the difference in index of the immersion liquid 38 and the average index of refraction of the tissue multiplied by the corrugation height h that is the optical path distance between the hills and valleys of the corrugation), not exceed a quarter wavelength of the laser beam which is used for imaging in the imaging system 36. Thus, the immersion medium is selected for the tissue type which is placed in the tray and substantially corrects for optical distortion due to the surface texture of the specimen.
  • [0031]
    In operation, the tray and the specimen are prepared by placing the tissue therein, and positioning the tissue. The tray is then placed in the support 12 and the magnet lock in 56 connects the tray to the objective lens. The lid is closed, piercing the membrane or plug covering opening 46, allowing coupling media to flow upwardly from the bag 44 attached to the bottom portion of tray 10. In an alternative embodiment, the tray may be filled with the immersion liquid before closing the lid and flows down into bag 44 upon lid closing. Then, under operation of the user controls 64, the beam is focused at the section and scanned across the section so as to obtain images of that section. A bar code, or other indicia, may be applied to the tray 10, as on its cover or a side wall for identification and tracking of the specimen, which is especially useful when the tray and specimen are archived (stored for later examination or other use).
  • [0032]
    Variations and modifications in the herein described apparatus and its method of operation, within the scope of the invention, will undoubtedly suggest themselves to those skilled in the art. Accordingly, the foregoing description should be taken as illustrative and not in a limiting sense.

Claims (31)

  1. 1-8. (canceled)
  2. 9. An apparatus for imaging excised tissue having a refractive index comprising:
    a tray upon which excised tissue of at least several millimeters in thickness is disposed, the tray including a window;
    means for clamping said excised tissue upon said tray to keep said tissue stationary and against said window; and
    optics directed towards the window and the excised tissue for imaging the tissue through the window, said tray containing an immersion medium having a refractive index matching the refractive index of said excised tissue.
  3. 10. The apparatus according to claim 9 further comprising means disposed between said tray and said optics which presents a medium between said tray and said optics optically coupling said optics to said tray.
  4. 11. The apparatus according to claim 9 further comprising indicia applied to said tray for identification of said excised tissue disposed therein.
  5. 12-20. (canceled)
  6. 21. The apparatus according to claim 9 wherein said clamping means has a mesh capable of holding said excised tissue upon said tray.
  7. 22. The apparatus according to claim 9 wherein said clamping means has a membrane capable of holding said excised tissue upon said tray.
  8. 23. The apparatus according to claim 9 wherein said clamping means comprises a clamp having at least one finger for clamping said excised tissue to hold said tissue stationary and against said window.
  9. 24. A holder to support excised tissue during imaging of said excised tissue, said holder comprising a container having a window upon which said excised tissue is adapted to be disposed, and a clamping member in said container capable of restraining said excised tissue in a position against said window, wherein said excised tissue is imagable through said window.
  10. 25. The holder according to claim 24 wherein said clamping member comprises a finger capable of restraining said excised tissue in a position against said window.
  11. 26. The holder according to claim 24 wherein said clamping member comprises a mesh capable of restraining said excised tissue in a position against said window.
  12. 27. The holder according to claim 24 wherein said clamping member comprises a membrane capable of restraining said excised tissue in a position against said window.
  13. 28. A method for imaging excised tissue comprising the steps of:
    providing a container having a surface for placement of said tissue;
    restraining said tissue in said container against said surface to keep said tissue stationary and against said surface; and
    imaging said tissue through at least part of said surface against which said tissue is held.
  14. 29. The method according to claim 28 wherein said restraining step is carried out with the aid of a clamping member located within said container.
  15. 30. The method according to claim 29 wherein said clamping member comprises a finger.
  16. 31. The method according to claim 28 wherein said restraining step is carried out with the aid of a mesh in said container.
  17. 32. The method according to claim 28 wherein said restraining step is carried out with the aid of a membrane in said container.
  18. 33. The method according to claim 28 wherein a plurality of the containers of different container types are used to image respective different types of excised tissues, each container type having associated therewith a respective liquid having a refractive index, the respective liquid being positioned in an imaging path for imaging of the respective tissue and the refractive index of the respective liquid associated with a respective one of said plurality of containers being selected in accordance with the respective refractive index of the type of said excised tissue.
  19. 34. The method according to claim 33 wherein one of said container types is used to image excised tissue from a kidney and includes a respective first liquid having an index of refraction suitable for imaging excised tissue from the kidney.
  20. 35. The method according to claims 34 wherein a second of said container types is used to image excised tissue from a liver and includes a respective second liquid, of different refractive index from that of said first liquid, and suitable for imaging excised tissue from the liver.
  21. 36. The method according to claim 35 wherein a third of said container types is used to image excised tissue from a cervix and includes a respective third liquid, of different refractive index from that of said first liquid and said second liquid, and suitable for imaging excised tissue from the cervix.
  22. 37. The method according to claim 33 wherein the liquid also serves as a tissue preservative or fixative.
  23. 38. The method according to claim 28 wherein said container includes an immersion medium having a refractive index suitable as a match for the refractive index of said excised tissue.
  24. 39. The method according to claim 33 wherein each container type includes a window having a first surface upon which the respective excised tissue is engaged during imaging, the respective refractive indices of the windows of the different container types being different from each other and being dependent upon the type of tissue for imaging using the respective container type.
  25. 40. The method according to claim 39 wherein imaging is made by a laser beam.
  26. 41. The method according to claim 40 wherein imaging is made of portions of the tissue several millimeters from the first surface.
  27. 42. The method according to claim 28 wherein imaging is made of portions of the tissue several millimeters from the surface.
  28. 43. For use with an imaging system, a tray system for imaging excised tissues having different respective refractive indices, the tray system comprising:
    a plurality of trays of different tray types, each tray type having associated therewith a respective medium having a different refractive index from that of the other tray types, the respective medium being adapted to be positioned in an imaging path of the imaging system, the refractive index of a respective medium associated with a respective one of said plurality of trays being selected in accordance with the respective refractive index of the type of excised tissue.
  29. 44. The tray system of claim 43 wherein each tray includes a clamping member for holding a surface of the excised tissue against a surface of the tray for imaging of the surface of the excised tissue.
  30. 45. The tray system of claim 44 wherein the respective media of the different tray types comprise liquids of different refractive indices.
  31. 46. An imaging system for imaging excised tissues having different respective refractive indices, the imaging system comprising:
    a plurality of trays of different tray types, each tray of each tray type having a surface against which can excised tissue is adapted to be supported for imaging and each tray type having associated therewith a respective medium having a different refractive index from that of the other tray types, the respective medium being adapted to be positioned in an imaging path of the imaging system, the refractive index of a respective medium associated with a respective one of said plurality of trays being selected in accordance with the respective refractive index of the type of excised tissue; and
    optics directed towards an excised tissue and establishing the imaging path for imaging the excised tissue through the surface of the tray.
US11020387 1999-02-17 2004-12-22 Tissue specimen holder Active US9128024B2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12047099 true 1999-02-17 1999-02-17
US09506135 US6330106B1 (en) 1999-02-17 2000-02-17 Tissue specimen holder
US09973109 US6856458B2 (en) 1999-02-17 2001-10-09 Tissue specimen holder
US11020387 US9128024B2 (en) 1999-02-17 2004-12-22 Tissue specimen holder

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11020387 US9128024B2 (en) 1999-02-17 2004-12-22 Tissue specimen holder

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US09973109 Continuation US6856458B2 (en) 1999-02-17 2001-10-09 Tissue specimen holder

Publications (2)

Publication Number Publication Date
US20050157386A1 true true US20050157386A1 (en) 2005-07-21
US9128024B2 US9128024B2 (en) 2015-09-08

Family

ID=22390502

Family Applications (3)

Application Number Title Priority Date Filing Date
US09506135 Active US6330106B1 (en) 1999-02-17 2000-02-17 Tissue specimen holder
US09973109 Active US6856458B2 (en) 1999-02-17 2001-10-09 Tissue specimen holder
US11020387 Active US9128024B2 (en) 1999-02-17 2004-12-22 Tissue specimen holder

Family Applications Before (2)

Application Number Title Priority Date Filing Date
US09506135 Active US6330106B1 (en) 1999-02-17 2000-02-17 Tissue specimen holder
US09973109 Active US6856458B2 (en) 1999-02-17 2001-10-09 Tissue specimen holder

Country Status (5)

Country Link
US (3) US6330106B1 (en)
EP (1) EP1161702B1 (en)
JP (1) JP2002537579A (en)
ES (1) ES2520140T3 (en)
WO (1) WO2000049447A1 (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090046359A1 (en) * 2007-08-14 2009-02-19 Nikon Corporation Microscope
WO2010021744A1 (en) * 2008-08-21 2010-02-25 California Institute Of Technology Microscope coupled tissue sectioning system
EP2615443A1 (en) * 2012-01-16 2013-07-17 MAVIG GmbH Method for preparing a tissue sample
US8764681B2 (en) 2011-12-14 2014-07-01 California Institute Of Technology Sharp tip carbon nanotube microneedle devices and their fabrication
DE102013210269B3 (en) * 2013-06-03 2014-11-06 Laser Zentrum Hannover E.V. Apparatus and method for analyzing samples in a liquid
US8976507B2 (en) 2011-03-29 2015-03-10 California Institute Of Technology Method to increase the capacitance of electrochemical carbon nanotube capacitors by conformal deposition of nanoparticles
US9050444B2 (en) 2007-07-10 2015-06-09 California Institute Of Technology Drug delivery and substance transfer facilitated by nano-enhanced device having aligned carbon nanotubes protruding from device surface
US9115424B2 (en) 2010-04-07 2015-08-25 California Institute Of Technology Simple method for producing superhydrophobic carbon nanotube array
US9349543B2 (en) 2012-07-30 2016-05-24 California Institute Of Technology Nano tri-carbon composite systems and manufacture
US9449816B2 (en) 2010-12-10 2016-09-20 California Institute Of Technology Method for producing graphene oxide with tunable gap
US9484543B2 (en) 2007-07-10 2016-11-01 California Institute Of Technology Fabrication of anchored carbon nanotube array devices for integrated light collection and energy conversion
US9677869B2 (en) 2012-12-05 2017-06-13 Perimeter Medical Imaging, Inc. System and method for generating a wide-field OCT image of a portion of a sample

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7227630B1 (en) 1998-09-14 2007-06-05 Lucid, Inc. Imaging of surgical biopsies
EP1121052A4 (en) 1998-09-14 2006-05-10 Lucid Inc Imaging of surgical biopsies
EP1161702B1 (en) * 1999-02-17 2014-07-23 Lucid, Inc. Tissue specimen holder
JP4564664B2 (en) * 1999-02-17 2010-10-20 ルーシド インコーポレーテッド Cassette for forming an optical thin fragments retained tissue specimen
US7194118B1 (en) 2000-11-10 2007-03-20 Lucid, Inc. System for optically sectioning and mapping surgically excised tissue
GB2360842B (en) * 2000-03-31 2002-06-26 Toshiba Res Europ Ltd An apparatus and method for investigating a sample
US20040133112A1 (en) * 2002-03-08 2004-07-08 Milind Rajadhyaksha System and method for macroscopic and confocal imaging of tissue
JP4555568B2 (en) * 2001-11-09 2010-10-06 株式会社半導体エネルギー研究所 Laser processing apparatus, laser processing method, and a thin film transistor manufacturing method of
US20070255169A1 (en) * 2001-11-19 2007-11-01 Dune Medical Devices Ltd. Clean margin assessment tool
US7197355B2 (en) * 2002-04-19 2007-03-27 Visiongate, Inc. Variable-motion optical tomography of small objects
DE10239739B4 (en) * 2002-08-29 2006-05-11 Leica Mikrosysteme Gmbh Apparatus and method for performing immunological labeling techniques for thin tissue sections
KR20050114615A (en) * 2003-02-04 2005-12-06 제테틱 인스티튜트 Compensation for effects of mismatch in indices of refraction at a substrate-medium interface in non-confocal, confocal, and interferometric confocal microscopy
US7026259B2 (en) * 2004-01-21 2006-04-11 International Business Machines Corporation Liquid-filled balloons for immersion lithography
WO2005078503A1 (en) * 2004-02-16 2005-08-25 Olympus Corporation Immersion object lens, immersion medium retaining mechanism and production method therefor
JP4731847B2 (en) * 2004-07-15 2011-07-27 オリンパス株式会社 Petri dishes, chamber unit, an optical microscopy method and sample analyzing method
US7864996B2 (en) * 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
US8260401B2 (en) 2006-07-26 2012-09-04 University Of Rochester Non-invasive in-vivo imaging of mechanoreceptors in skin using confocal microscopy
US7973925B2 (en) * 2007-02-06 2011-07-05 C8 Medisensors Inc. Apparatus for stabilizing mechanical, thermal, and optical properties and for reducing the fluorescence of biological samples for optical evaluation
DE202008010411U1 (en) 2008-05-31 2008-10-16 Klinika Medical Gmbh Arrangement for transportation and / or storage of a human or animal tissue sample
JP5638793B2 (en) * 2009-12-03 2014-12-10 オリンパス株式会社 Microscope apparatus
US8411265B2 (en) * 2011-06-14 2013-04-02 C8 Medisensors Inc. Apparatus for stabilizing mechanical, thermal, and optical properties and for reducing the fluorescence of biological samples for optical evaluation
EP2817609A4 (en) * 2012-02-26 2015-10-28 Caliber Imaging & Diagnostics Inc Tissue specimen stage for an optical sectioning microscope
EP2873997A1 (en) * 2013-11-15 2015-05-20 Eppendorf Ag Counting chamber device, positioning device for this, counting device and method for counting microscopic particles
WO2016126861A1 (en) * 2015-02-04 2016-08-11 Apollo Medical Optics Inc. Optical coherence tomography apparatus and its application
DE102015204134A1 (en) * 2015-03-09 2016-09-15 Carl Zeiss Microscopy Gmbh Microscope for spherically corrected image three-dimensional objects
GB201515961D0 (en) * 2015-09-09 2015-10-21 Secr Defence A controlled atmosphere bag suitable for microscopy and spectroscopy
CN105784457B (en) * 2016-03-21 2018-04-20 中国医科大学附属第医院 Cryogenic storage of tissue samples embedded systems

Citations (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US568741A (en) * 1896-10-06 Henry r
US1002910A (en) * 1911-01-21 1911-09-12 Foote Mineral Co Display-mount.
US1991983A (en) * 1932-08-25 1935-02-19 Joseph S Newman Microscope slide
US3031924A (en) * 1959-03-12 1962-05-01 James C Lamal Observation slide
US3202049A (en) * 1961-11-29 1965-08-24 Bausch & Lomb Liquid dispenser for microscope objective
US3510194A (en) * 1965-08-09 1970-05-05 Robert F Connelly Particle count membrane filter mount
US3551023A (en) * 1969-01-17 1970-12-29 Ibm Pathology specimen processing method and article
US3556633A (en) * 1969-01-17 1971-01-19 Winifred Liu Mutschmann Specimen carrying slide with runoff trough
US3648587A (en) * 1967-10-20 1972-03-14 Eastman Kodak Co Focus control for optical instruments
US3904781A (en) * 1973-10-15 1975-09-09 Donald E Henry Method of preparing cells for inspection
US4159875A (en) * 1976-10-21 1979-07-03 Abbott Laboratories Specimen holder
US4208101A (en) * 1976-12-04 1980-06-17 Carl Zeiss-Stiftung Immersion objective for a microscope
US4257346A (en) * 1976-12-10 1981-03-24 Technicon Instruments Corp. Apparatus for mounting tissue sections with an U.V. light curable mounting medium
US4545831A (en) * 1982-09-13 1985-10-08 The Mount Sinai School Of Medicine Method for transferring a thin tissue section
US4744643A (en) * 1987-01-20 1988-05-17 Taylor Howard L Apparatus for restricting motion of living microscopic organisms during observation under a microscope
US4752347A (en) * 1986-10-03 1988-06-21 Rada David C Apparatus for preparing tissue sections
US4965441A (en) * 1988-01-27 1990-10-23 Commissariat A L'energie Atomique Method for the scanning confocal light-optical microscopic and indepth examination of an extended field and devices for implementing said method
US4974952A (en) * 1988-03-31 1990-12-04 Focht Daniel C Live cell chamber for microscopes
US5034613A (en) * 1989-11-14 1991-07-23 Cornell Research Foundation, Inc. Two-photon laser microscopy
US5120953A (en) * 1988-07-13 1992-06-09 Harris Martin R Scanning confocal microscope including a single fibre for transmitting light to and receiving light from an object
US5122653A (en) * 1989-08-22 1992-06-16 Nikon Corporation Confocal type laser scan microscope with integrated illumination, detection and waveguide system
USRE34214E (en) * 1984-03-15 1993-04-06 Molecular Dynamics, Inc. Method and apparatus for microphotometering microscope specimens
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5296963A (en) * 1991-02-27 1994-03-22 Hitachi, Ltd. Apparatus and method for applying a laser beam through a microscope
US5311358A (en) * 1992-10-08 1994-05-10 Time Surgical, Inc. Universal microscope drape
US5367401A (en) * 1990-11-23 1994-11-22 Perceptive Scientific Instruments, Inc. Microscope slide rotary stage
US5383472A (en) * 1993-07-22 1995-01-24 Devlin; Mark T. Method and apparatus for handling of biopsy tissue specimen
US5503741A (en) * 1993-09-22 1996-04-02 Pierce Chemical Company Dialysis device with hermetically sealed vacant chamber
US5532874A (en) * 1992-12-18 1996-07-02 Morphometrix Inc. Multiscanning confocal microscopy
US5675700A (en) * 1993-02-16 1997-10-07 The Perkin-Elmer Corporation Assembly system for in situ per amplification
US5719700A (en) * 1991-10-11 1998-02-17 L'oreal Apparatus for in vivo observation of the microscopic structure of the skin or of a similar tissue
US5788639A (en) * 1995-07-13 1998-08-04 Lucid Technologies, Inc. Confocal imaging through thick dermal tissue
US5812312A (en) * 1997-09-04 1998-09-22 Lorincz; Andrew Endre Microscope slide
US5836877A (en) * 1997-02-24 1998-11-17 Lucid Inc System for facilitating pathological examination of a lesion in tissue
US5843674A (en) * 1993-11-16 1998-12-01 Pola Chemical Industries Inc. Anti-human tyrosinase monoclonal antibody
US5870223A (en) * 1996-07-22 1999-02-09 Nikon Corporation Microscope system for liquid immersion observation
US5880880A (en) * 1995-01-13 1999-03-09 The General Hospital Corp. Three-dimensional scanning confocal laser microscope
US6048723A (en) * 1997-12-02 2000-04-11 Flexcell International Corporation Flexible bottom culture plate for applying mechanical load to cell cultures
US6272235B1 (en) * 1997-03-03 2001-08-07 Bacus Research Laboratories, Inc. Method and apparatus for creating a virtual microscope slide
US6330106B1 (en) * 1999-02-17 2001-12-11 Lucid, Inc. Tissue specimen holder
US6411434B1 (en) * 1999-02-17 2002-06-25 Lucid, Inc. Cassette for facilitating optical sectioning of a retained tissue specimen
US6493460B1 (en) * 1992-10-14 2002-12-10 Monogen, Inc. Method and apparatus for automatically detecting malignancy-associated changes

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1472294A1 (en) * 1966-09-07 1969-02-13 Leitz Ernst Gmbh Immersionskoerper
DE2210442A1 (en) * 1972-03-03 1973-09-06 Remy Ernst Dr tiefgekuehlter samples microscope arrangement for examination, having disposed between the microscope objective and the sample or probe cover immersionsfluessigkeit
JPS6116048B2 (en) * 1980-02-13 1986-04-28 Mitsubishi Electric Corp
DE3220702A1 (en) * 1982-06-02 1983-12-08 Manfred Fink Clamping device for samples to be examined microscopically, with background neutralisation
DE3718066A1 (en) * 1987-05-29 1988-12-08 Zeiss Carl Fa A method for microinjection into cells or for suction from individual cells or whole culture cells
US5681741A (en) * 1993-02-16 1997-10-28 The Perkin-Elmer Corporation In situ PCR amplification system
JPH07140393A (en) * 1993-11-22 1995-06-02 Ishikawajima Harima Heavy Ind Co Ltd Glass layer thickness correcting device for microscope
WO1996021938A1 (en) 1995-01-13 1996-07-18 The General Hospital Corporation Video-rate confocal scanning laser microscope
JPH1039220A (en) * 1996-07-22 1998-02-13 Nikon Corp microscope
JPH1039222A (en) * 1996-07-23 1998-02-13 Nikon Corp microscope

Patent Citations (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US568741A (en) * 1896-10-06 Henry r
US1002910A (en) * 1911-01-21 1911-09-12 Foote Mineral Co Display-mount.
US1991983A (en) * 1932-08-25 1935-02-19 Joseph S Newman Microscope slide
US3031924A (en) * 1959-03-12 1962-05-01 James C Lamal Observation slide
US3202049A (en) * 1961-11-29 1965-08-24 Bausch & Lomb Liquid dispenser for microscope objective
US3510194A (en) * 1965-08-09 1970-05-05 Robert F Connelly Particle count membrane filter mount
US3648587A (en) * 1967-10-20 1972-03-14 Eastman Kodak Co Focus control for optical instruments
US3551023A (en) * 1969-01-17 1970-12-29 Ibm Pathology specimen processing method and article
US3556633A (en) * 1969-01-17 1971-01-19 Winifred Liu Mutschmann Specimen carrying slide with runoff trough
US3904781A (en) * 1973-10-15 1975-09-09 Donald E Henry Method of preparing cells for inspection
US4159875A (en) * 1976-10-21 1979-07-03 Abbott Laboratories Specimen holder
US4208101A (en) * 1976-12-04 1980-06-17 Carl Zeiss-Stiftung Immersion objective for a microscope
US4257346A (en) * 1976-12-10 1981-03-24 Technicon Instruments Corp. Apparatus for mounting tissue sections with an U.V. light curable mounting medium
US4545831A (en) * 1982-09-13 1985-10-08 The Mount Sinai School Of Medicine Method for transferring a thin tissue section
USRE34214E (en) * 1984-03-15 1993-04-06 Molecular Dynamics, Inc. Method and apparatus for microphotometering microscope specimens
US4752347A (en) * 1986-10-03 1988-06-21 Rada David C Apparatus for preparing tissue sections
US4744643A (en) * 1987-01-20 1988-05-17 Taylor Howard L Apparatus for restricting motion of living microscopic organisms during observation under a microscope
US4965441A (en) * 1988-01-27 1990-10-23 Commissariat A L'energie Atomique Method for the scanning confocal light-optical microscopic and indepth examination of an extended field and devices for implementing said method
US4974952A (en) * 1988-03-31 1990-12-04 Focht Daniel C Live cell chamber for microscopes
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5120953A (en) * 1988-07-13 1992-06-09 Harris Martin R Scanning confocal microscope including a single fibre for transmitting light to and receiving light from an object
US5122653A (en) * 1989-08-22 1992-06-16 Nikon Corporation Confocal type laser scan microscope with integrated illumination, detection and waveguide system
US5034613A (en) * 1989-11-14 1991-07-23 Cornell Research Foundation, Inc. Two-photon laser microscopy
US5367401A (en) * 1990-11-23 1994-11-22 Perceptive Scientific Instruments, Inc. Microscope slide rotary stage
US5296963A (en) * 1991-02-27 1994-03-22 Hitachi, Ltd. Apparatus and method for applying a laser beam through a microscope
US5719700A (en) * 1991-10-11 1998-02-17 L'oreal Apparatus for in vivo observation of the microscopic structure of the skin or of a similar tissue
US5311358A (en) * 1992-10-08 1994-05-10 Time Surgical, Inc. Universal microscope drape
US6493460B1 (en) * 1992-10-14 2002-12-10 Monogen, Inc. Method and apparatus for automatically detecting malignancy-associated changes
US5532874A (en) * 1992-12-18 1996-07-02 Morphometrix Inc. Multiscanning confocal microscopy
US5675700A (en) * 1993-02-16 1997-10-07 The Perkin-Elmer Corporation Assembly system for in situ per amplification
US5383472A (en) * 1993-07-22 1995-01-24 Devlin; Mark T. Method and apparatus for handling of biopsy tissue specimen
US5503741A (en) * 1993-09-22 1996-04-02 Pierce Chemical Company Dialysis device with hermetically sealed vacant chamber
US5843674A (en) * 1993-11-16 1998-12-01 Pola Chemical Industries Inc. Anti-human tyrosinase monoclonal antibody
US5995283A (en) * 1995-01-13 1999-11-30 General Hospital Corporation Three-dimensional scanning confocal laser microscope
US5880880A (en) * 1995-01-13 1999-03-09 The General Hospital Corp. Three-dimensional scanning confocal laser microscope
US5788639A (en) * 1995-07-13 1998-08-04 Lucid Technologies, Inc. Confocal imaging through thick dermal tissue
US5870223A (en) * 1996-07-22 1999-02-09 Nikon Corporation Microscope system for liquid immersion observation
US5836877A (en) * 1997-02-24 1998-11-17 Lucid Inc System for facilitating pathological examination of a lesion in tissue
US6272235B1 (en) * 1997-03-03 2001-08-07 Bacus Research Laboratories, Inc. Method and apparatus for creating a virtual microscope slide
US5812312A (en) * 1997-09-04 1998-09-22 Lorincz; Andrew Endre Microscope slide
US6048723A (en) * 1997-12-02 2000-04-11 Flexcell International Corporation Flexible bottom culture plate for applying mechanical load to cell cultures
US6330106B1 (en) * 1999-02-17 2001-12-11 Lucid, Inc. Tissue specimen holder
US6411434B1 (en) * 1999-02-17 2002-06-25 Lucid, Inc. Cassette for facilitating optical sectioning of a retained tissue specimen
US6856458B2 (en) * 1999-02-17 2005-02-15 Lucid, Inc. Tissue specimen holder

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9050444B2 (en) 2007-07-10 2015-06-09 California Institute Of Technology Drug delivery and substance transfer facilitated by nano-enhanced device having aligned carbon nanotubes protruding from device surface
US9484543B2 (en) 2007-07-10 2016-11-01 California Institute Of Technology Fabrication of anchored carbon nanotube array devices for integrated light collection and energy conversion
US9352136B2 (en) 2007-07-10 2016-05-31 California Institute Of Technology Drug delivery and substance transfer facilitated by nano-enhanced device having aligned carbon nanotubes protruding from device surface
US20090046359A1 (en) * 2007-08-14 2009-02-19 Nikon Corporation Microscope
WO2010021744A1 (en) * 2008-08-21 2010-02-25 California Institute Of Technology Microscope coupled tissue sectioning system
US9115424B2 (en) 2010-04-07 2015-08-25 California Institute Of Technology Simple method for producing superhydrophobic carbon nanotube array
US9449816B2 (en) 2010-12-10 2016-09-20 California Institute Of Technology Method for producing graphene oxide with tunable gap
US8976507B2 (en) 2011-03-29 2015-03-10 California Institute Of Technology Method to increase the capacitance of electrochemical carbon nanotube capacitors by conformal deposition of nanoparticles
US8764681B2 (en) 2011-12-14 2014-07-01 California Institute Of Technology Sharp tip carbon nanotube microneedle devices and their fabrication
EP2615443A1 (en) * 2012-01-16 2013-07-17 MAVIG GmbH Method for preparing a tissue sample
WO2013107753A1 (en) * 2012-01-16 2013-07-25 Mavig Gmbh Method for preparing a tissue sample
US9349543B2 (en) 2012-07-30 2016-05-24 California Institute Of Technology Nano tri-carbon composite systems and manufacture
US9677869B2 (en) 2012-12-05 2017-06-13 Perimeter Medical Imaging, Inc. System and method for generating a wide-field OCT image of a portion of a sample
DE102013210269B3 (en) * 2013-06-03 2014-11-06 Laser Zentrum Hannover E.V. Apparatus and method for analyzing samples in a liquid
DE102013210269B8 (en) 2013-06-03 2015-01-29 Laser Zentrum Hannover E.V. Apparatus and method for analyzing samples in a liquid
WO2014195005A1 (en) 2013-06-03 2014-12-11 Laser Zentrum Hannover E.V. Device and method for examining samples in a liquid

Also Published As

Publication number Publication date Type
EP1161702A4 (en) 2007-10-24 application
JP2002537579A (en) 2002-11-05 application
US9128024B2 (en) 2015-09-08 grant
US6856458B2 (en) 2005-02-15 grant
US20020101655A1 (en) 2002-08-01 application
WO2000049447A1 (en) 2000-08-24 application
US6330106B1 (en) 2001-12-11 grant
ES2520140T3 (en) 2014-11-11 grant
JP2002537579U (en) application
EP1161702B1 (en) 2014-07-23 grant
EP1161702A1 (en) 2001-12-12 application

Similar Documents

Publication Publication Date Title
Neil et al. Method of obtaining optical sectioning by using structured light in a conventional microscope
US6320174B1 (en) Composing microscope
US5633504A (en) Inspection of optical components
US5777783A (en) Microscope
Shotton et al. Confocal scanning microscopy: three-dimensional biological imaging
US4887892A (en) Method and method and apparatus for control of light intensity for image analysis
US3820879A (en) Biomicroscopic eye contact glass
US5365562A (en) Digital imaging apparatus
Petráň et al. The tandem scanning reflected light microscope
Hibbs Confocal microscopy for biologists
US6301005B1 (en) Inspection system for optical components
US4777525A (en) Apparatus and method for a multi-resolution electro-optical imaging, display and storage/retrieval system
Mertz et al. Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection
US5212571A (en) Ultrasonic holographic imaging apparatus having zoom feature
US5859364A (en) Scanning probe microscope
US20050085721A1 (en) System and method for processing specimens and images for optical tomography
Eckard et al. In vivo investigations of the corneal epithelium with the confocal Rostock Laser Scanning Microscope (RLSM)
US20080247038A1 (en) Scanning confocal microscope
US5675145A (en) Scanning probe microscope having an optical system for enabling identification of the scanning region and sample observation during a scanning operation
US20040101210A1 (en) Miniaturized microscope array digital slide scanner
US5677525A (en) Ancillary module for making a spatially-resolved measurement of a focus volume
US5781303A (en) Method for determining the thickness of an optical sample
US20100177190A1 (en) Microscopy system with revolvable stage
US20010045506A1 (en) Electronic camera for microscope
US4836667A (en) Microscope

Legal Events

Date Code Title Description
AS Assignment

Owner name: NORTHEAST LCD CAPITAL, LLC, MAINE

Free format text: SECURITY AGREEMENT;ASSIGNOR:LUCID, INC.;REEL/FRAME:028533/0017

Effective date: 20120705

AS Assignment

Owner name: LUCID, INC., NEW YORK

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:GREENWALD, ROGER J.;ZAVISLAN, JAMES M.;SIGNING DATES FROM 20000428 TO 20000503;REEL/FRAME:037904/0865

AS Assignment

Owner name: CALIBER IMAGING & DIAGNOSTICS, INC., NEW YORK

Free format text: CHANGE OF NAME;ASSIGNOR:LUCID, INC.;REEL/FRAME:038226/0225

Effective date: 20141120