US20040038340A1 - Mutants of lactobacillus casei defective in carbon catabolism regulation - Google Patents

Mutants of lactobacillus casei defective in carbon catabolism regulation Download PDF

Info

Publication number
US20040038340A1
US20040038340A1 US10/240,280 US24028003A US2004038340A1 US 20040038340 A1 US20040038340 A1 US 20040038340A1 US 24028003 A US24028003 A US 24028003A US 2004038340 A1 US2004038340 A1 US 2004038340A1
Authority
US
United States
Prior art keywords
mutant
mutation
gene
casei
hpr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/240,280
Other languages
English (en)
Inventor
Josef Deutscher
Laurent Benbadis
Anne Pierson
Jean-Michel Faurie
Gaspar Perez
Vicente Monedero
Rosa Viana
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Centre National de la Recherche Scientifique CNRS
Gervais Danone SA
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Centre National de la Recherche Scientifique CNRS
Gervais Danone SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Centre National de la Recherche Scientifique CNRS, Gervais Danone SA filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Assigned to CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC), COMPAGNIE GERVAIS DANONE reassignment CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: MONEDERO GARCIA, VICENTE, PEREZ MARTINEZ, GASPAR, VIANA BALLESTER, ROSA, DEUTSCHER, JOSEF, PIERSON, ANNE, FAURIE, JEAN-MICHEL, BENBADIS, LAURENT
Publication of US20040038340A1 publication Critical patent/US20040038340A1/en
Priority to US12/414,246 priority Critical patent/US8551729B2/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/335—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/245—Lactobacillus casei

Definitions

  • Carbon catabolite repression is a regulatory mechanism allowing bacteria to choose between different carbon sources according to their metabolic value and to switch from a carbon source to another depending on their availability in the growth medium.
  • a well-known manifestation of catabolic repression is the diauxic growth that occurs when bacteria are grown in presence of both glucose and lactose. Diauxic growth curves show two distinct phases of exponential growth, separated by a lag phase. During the first phase of growth, glucose represses the synthesis of the enzymes necessary for lactose utilisation, and is therefore the only source of energy of the bacteria. When all the glucose is exhausted occurs the lag phase, during which the enzymes for lactose utilisation are synthesised, allowing lactose to be used as a source of energy during the second phase of growth.
  • a main target of catabolite repression is the transport of sugars into the bacterial cell. In L. casei , this transport is predominantly performed by the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS).
  • PTS phosphoenolpyruvate:carbohydrate phosphotransferase system
  • the PTS of gram-positive bacteria has been studied mainly in Bacillus subtilis ; it has been shown that it effects the phosphorylation of sugars and their transfer into the cell through a cascade of phosphorylations involving the general non-sugar-specific enzymes EI and HPr, and the sugar-specific enzymes EIIA, EIIB, and EIIC.
  • the first step is the phosphorylation of EI from phosphoenolpyruvate (PEP).
  • the phosphorylated EI (EI-P) catalyses the phosphorylation of HPr, at the catalytic His-15.
  • CcpA catabolite control protein
  • P-Ser-HPr catabolite response elements
  • nucleotidic sequence of the ptsHI operon, and the peptidic sequences of HPr and EI of L. casei are respectively disclosed in the enclosed sequence listing under the identifiers SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO: b 3 .
  • sequence of the hprK gene is available in GENBANK under the access number Y18948.
  • mutations in genes encoding antiterminators or transcriptional activators having the PTS regulation domain for instance any mutation impairing the phosphorylation of any of these antiterminators or transcriptional activators by P-His-HPr and/or by P-EIIB or mimicking the phosphorylated form of the antiterminator (for example phosphorylatable histidyl residues mutated to Asp or Glu);
  • This mutant results from transformation of L. casei wild-type strain BL23 with plasmid pVME800, as described in Example 1 above.
  • ohprKLc4 (5′-ATTGAAAAGAGCTCGGATTAAGTGCT-3′).
  • ohprKLc5 (5′-CCCCTCGAGGTCGACGGTATGGATAAGCTTGA-3′);
  • HPr kinase and P-Ser-HPr phosphatase activities were determined in crude extracts of L. casei wild-type and pHKLc208(Am) integrants.
  • P-Ser-HPr phosphatase assays were carried out by incubating a 20 ⁇ l assay mixture containing 10 ⁇ l crude extract, 2.5 ⁇ g B. subtilis P-Ser-HPr(His) 6 , 20 mM sodium phosphate, pH 7.2, 10 MM MgCl 2 and 50 mM Tris-HCl, pH 7.4, for 10 min at 37° C. The dephosphorylation reaction was stopped by heat inactivation at 65° C. for 5 min. An equal volume of sample buffer was added to the assay mixtures before separating HPr and P-Ser-HPr on a 12.5% non-denaturing polyacrylamide gel.
  • the fermented products obtained from standardized milk supplemented with glucose with the mutant strain ptsI have a gel-strength lower of about 15-25% than the fermented products obtained from the wild-type strain. This allows to obtain a more elastic gel of about 15-25% and to reduce syneresis.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • General Preparation And Processing Of Foods (AREA)
US10/240,280 2000-03-31 2001-03-30 Mutants of lactobacillus casei defective in carbon catabolism regulation Abandoned US20040038340A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/414,246 US8551729B2 (en) 2000-03-31 2009-03-30 Mutants of Lactobacillus casei defective in carbon catabolism regulation

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00400894 2000-03-31
EP00400894.2 2000-03-31
PCT/EP2001/003951 WO2001073039A1 (en) 2000-03-31 2001-03-30 Mutants of lactobacillus casei defective in carbon catabolism regulation

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/414,246 Division US8551729B2 (en) 2000-03-31 2009-03-30 Mutants of Lactobacillus casei defective in carbon catabolism regulation

Publications (1)

Publication Number Publication Date
US20040038340A1 true US20040038340A1 (en) 2004-02-26

Family

ID=8173623

Family Applications (2)

Application Number Title Priority Date Filing Date
US10/240,280 Abandoned US20040038340A1 (en) 2000-03-31 2001-03-30 Mutants of lactobacillus casei defective in carbon catabolism regulation
US12/414,246 Expired - Fee Related US8551729B2 (en) 2000-03-31 2009-03-30 Mutants of Lactobacillus casei defective in carbon catabolism regulation

Family Applications After (1)

Application Number Title Priority Date Filing Date
US12/414,246 Expired - Fee Related US8551729B2 (en) 2000-03-31 2009-03-30 Mutants of Lactobacillus casei defective in carbon catabolism regulation

Country Status (9)

Country Link
US (2) US20040038340A1 (de)
EP (2) EP1268808B1 (de)
AT (2) ATE435287T1 (de)
AU (1) AU2001250416A1 (de)
CA (1) CA2404389C (de)
DE (2) DE60125155T2 (de)
DK (2) DK1268808T3 (de)
ES (2) ES2278737T3 (de)
WO (1) WO2001073039A1 (de)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060194226A1 (en) * 2005-01-12 2006-08-31 Osel, Inc. Modified cyanovirin protein
US20100056926A1 (en) * 2008-08-26 2010-03-04 Gynesonics, Inc. Ablation device with articulated imaging transducer
US20110045134A1 (en) * 2007-12-04 2011-02-24 Louise Perrier Use of L. Casei Ssp. Paracasei as Antifungal Agent
JP2012528582A (ja) * 2009-06-03 2012-11-15 ヨープレイ・フランス 発酵乳製品の製造方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2877012B1 (fr) * 2004-10-22 2010-09-10 Gervais Danone Sa Souches de streptococcus thermophilus ami deficientes a post-acidification reduite
CN102041267B (zh) * 2009-10-16 2014-06-04 中国科学院上海生命科学研究院 一种解除丙酮丁醇梭菌葡萄糖抑制效应的方法
SI24570A (sl) 2013-12-23 2015-06-30 Medis D.O.O. Novi sevi rodu Lactobacillus in njihova uporaba

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491079A (en) * 1992-04-07 1996-02-13 Nestec S.A. Promoterless foreign gene integrated in Streptococcus thermophilus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE391786T1 (de) * 1995-05-05 2008-04-15 Genencor Int Anwendung von glukosetransportmutanten tur herstellung von verbindungen des aromatischen syntheseweges
ATE253640T1 (de) * 1998-06-17 2003-11-15 Nestle Sa Mobile genetische elemente als werkzeuge zur genetischen modifikation von l. delbrueckii oder l. helveticus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491079A (en) * 1992-04-07 1996-02-13 Nestec S.A. Promoterless foreign gene integrated in Streptococcus thermophilus

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060194226A1 (en) * 2005-01-12 2006-08-31 Osel, Inc. Modified cyanovirin protein
US7456011B2 (en) * 2005-01-12 2008-11-25 Osel, Inc. Modified cyanovirin-n polypeptide
US20090155304A1 (en) * 2005-01-12 2009-06-18 Osel, Inc. Modified Cyanovirin-N Polypeptide
US20110045134A1 (en) * 2007-12-04 2011-02-24 Louise Perrier Use of L. Casei Ssp. Paracasei as Antifungal Agent
US9439445B2 (en) * 2007-12-04 2016-09-13 Compagnie Gervais Danone Use of L. casei ssp. paracasei as antifungal agent
US20100056926A1 (en) * 2008-08-26 2010-03-04 Gynesonics, Inc. Ablation device with articulated imaging transducer
JP2012528582A (ja) * 2009-06-03 2012-11-15 ヨープレイ・フランス 発酵乳製品の製造方法

Also Published As

Publication number Publication date
US8551729B2 (en) 2013-10-08
DE60139167D1 (de) 2009-08-13
CA2404389A1 (en) 2001-10-04
EP1268808A1 (de) 2003-01-02
CA2404389C (en) 2011-11-22
ATE348166T1 (de) 2007-01-15
DE60125155D1 (de) 2007-01-25
DK1268808T3 (da) 2007-04-10
ES2334050T3 (es) 2010-03-04
WO2001073039A1 (en) 2001-10-04
EP1808485B1 (de) 2009-07-01
EP1808485A3 (de) 2007-07-25
EP1808485A2 (de) 2007-07-18
ATE435287T1 (de) 2009-07-15
US20110212223A1 (en) 2011-09-01
DE60125155T2 (de) 2007-11-22
AU2001250416A1 (en) 2001-10-08
DK1808485T3 (da) 2009-11-02
EP1268808B1 (de) 2006-12-13
ES2278737T3 (es) 2007-08-16

Similar Documents

Publication Publication Date Title
Viana et al. Enzyme I and HPr from Lactobacillus casei: their role in sugar transport, carbon catabolite repression and inducer exclusion
US8551729B2 (en) Mutants of Lactobacillus casei defective in carbon catabolism regulation
Reverchon et al. The cyclic AMP receptor protein is the main activator of pectinolysis genes in Erwinia chrysanthemi
Monedero et al. Catabolite repression in Lactobacillus casei ATCC 393 is mediated by CcpA
Ripio et al. A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes
Dossonnet et al. Phosphorylation of HPr by the bifunctional HPr kinase/P-Ser-HPr phosphatase from Lactobacillus casei controls catabolite repression and inducer exclusion but not inducer expulsion
Jennings et al. Analysis of the Escherichia coli gene encoding L-asparaginase II, ansB, and its regulation by cyclic AMP receptor and FNR proteins
Aldridge et al. Genetics of sorbitol metabolism in Erwinia amylovora and its influence on bacterial virulence
Baptista et al. Mutations leading to increased levels of resistance to glycopeptide antibiotics in VanB‐type enterococci
Monedero et al. The phosphotransferase system of Lactobacillus casei: regulation of carbon metabolism and connection to cold shock response
WO2019122365A1 (en) New lactic acid bacteria with sweetening properties and uses thereof
EP2551279A1 (de) Mittels intestinalen Lactobacillus salivarius hergestelltes antimikrobielles Peptid
EP0579730A1 (de) Nisin a aehnliche lantibiotika, diese produzierende milchsaeurebakterien und deren herstellung
Chapot-Chartier et al. Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene
Mayo et al. Cloning and expression of the plasmid encoded β-D-galactosidase gene from a Lactobacillus plantarum strain of dairy origin
EP3898655A1 (de) Neue milchsäurebakterien
Stentz et al. Molecular cloning and analysis of the ptsHI operon in Lactobacillus sake
WO2001001786A2 (en) Methods and means for producing improved dairy products
Beaufils et al. The cold shock response of Lactobacillus casei: relation between HPr phosphorylation and resistance to freeze/thaw cycles
Yebra et al. An esterase gene from Lactobacillus casei cotranscribed with genes encoding a phosphoenolpyruvate: sugar phosphotransferase system and regulated by a LevR-like activator and σ54 factor
KR101940785B1 (ko) 써모토가 페트로필라 유래의 헥수론산 c4-에피머화 변이체 효소 및 이의 용도
Schrögel et al. Expression of a pepT homologue from Bacillus subtilis
WO2005090396A1 (en) Novel mannose-specific adhesins and their use
van den Bogaard Catabolite control of sugar metabolism in Streptococcus thermophilus
WO2001014520A2 (en) Phage resistant lactic acid bacterial mutants

Legal Events

Date Code Title Description
AS Assignment

Owner name: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (C

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DEUTSCHER, JOSEF;BENBADIS, LAURENT;PIERSON, ANNE;AND OTHERS;REEL/FRAME:013695/0798;SIGNING DATES FROM 20021011 TO 20021108

Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DEUTSCHER, JOSEF;BENBADIS, LAURENT;PIERSON, ANNE;AND OTHERS;REEL/FRAME:013695/0798;SIGNING DATES FROM 20021011 TO 20021108

Owner name: COMPAGNIE GERVAIS DANONE, FRANCE

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DEUTSCHER, JOSEF;BENBADIS, LAURENT;PIERSON, ANNE;AND OTHERS;REEL/FRAME:013695/0798;SIGNING DATES FROM 20021011 TO 20021108

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION