US20020076450A1 - Cosmetic containing plant extracts, particularly with a depigmenting, anti-radical and anit-ageing action - Google Patents
Cosmetic containing plant extracts, particularly with a depigmenting, anti-radical and anit-ageing action Download PDFInfo
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- US20020076450A1 US20020076450A1 US09/455,402 US45540299A US2002076450A1 US 20020076450 A1 US20020076450 A1 US 20020076450A1 US 45540299 A US45540299 A US 45540299A US 2002076450 A1 US2002076450 A1 US 2002076450A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Definitions
- the invention concerns the cosmetics, more particularly the dermo-cosmetics field, and its subject is the use of plant extracts, found especially in Haut-Maroni (French Guiana), in the preparation of cosmetics for the skin, mucous membrane and/or exoskeleton (hair, nails, . . . ).
- the plants are prepared by decoction then used in a hip bath according to the stage in he menstrual cycle and the required effect.
- extracts of some of the above-mentioned plants have not only the fore-mentioned pharmacological properties but also clear anti-free-radical, anti-ageing and glutathione auto-synthesis reducing properties and action, which are at least equivalent—if not superior—to those of the active ingredients normally used in cosmetics.
- the main subject of the invention is thus the use, as an active ingredient for the preparation of a cosmetic for topical use on the skin, mucous membrane and/or exoskeleton, of at least one extract or mixture of extracts from a plant selected from the group made up of Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus , particularly with an enhanced anti-radical and glutathione auto-synthesis reducing action.
- This action may in particular be advantageously used in anti-ageing, anti-physical-stress (UV-R), cold, heat, wind and anti-chemical stress (especially pollution) and light-protective cosmetics, in anti-stress and light-protective capillary preparations or in sun or after-sun products.
- UV-R anti-physical-stress
- UV-R cold, heat, wind and anti-chemical stress
- light-protective cosmetics in anti-stress and light-protective capillary preparations or in sun or after-sun products.
- the above-mentioned extracts may for example be prepared from the whole plant or plants.
- the plant extracts are advantageously obtained from the aerial parts of the plant, for example the stems, leaves, flowers and/or buds.
- the extracting solvent used may advantageously be chosen from the group formed by water, aqueous solutions with different pH levels, C1-C6 alcohols, ketones (acetone, methylketone, diethylketone), halogenated hydrocarbons, esters (ethyl acetate, propyl actetate, butyl acetate or the like), polyhydric alcohols (glycols, diethylene glycol, propane diol, dipropylene glycol, butylene glycol) or mixtures of at least two of the fore-mentioned substances.
- the plant extract(s) comprise one or more separated, purified fractions extracted from one or more of the plants.
- the active ingredient incorporated in it is advantageously at least one plant extract selected from the group formed by Spondias mombin, Maprounea guianensis, Gouania blanchetiana, Waltheria indica and Cordia schomburgkii.
- the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by of Spondias mombin, Maprounea guianensis, Waltheria indica and Randia armata.
- the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis and Waltheria indica.
- the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana and Randia armata.
- the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis and Gouania blanchetiana.
- the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Gouania blanchetiana, Cordia schomburgkii and Hibiscus furcellatus,
- the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by Spondias mombin, Maprounea gulanensis, Gouania blanchetiana and Cordia schomburgkii.
- the active ingredient will preferably be an extract or a mixture of extracts obtained from the Spondias mombin plant and/or the Maprounea guianensis plant.
- a method of preparing an aqueous extract may for example comprise the following stages:
- a method of preparing an aqueous alcohol extract may for example comprise the following stages:
- a method of preparing an alcohol extract may for example comprise the following stages:
- Dry leaves of Spondias mombin were treated by the operating methods previously described, to obtain aqueous, alcoholic and aqueous alcohol extracts.
- Aggressive action by free radicals is one of the main skin-damaging mechanisms owing to the action of stress factors in the external environment.
- Free radicals particularly the reactive forms of oxygen (superoxide radical O 2 ., hydroxyl radical HO., singlet oxygen O 2 1 , hydrogen peroxide H 2 O 2 ), act on a cellular scale: particularly damaging the membranous lipids with the formation of lipoperoxides, deterioration of proteins, blocking of enzyme systems and deterioration of the nuclear genetic capital. Noxious effects are also produced in the macromolecules of the extra-cellular matrix of the dermis, namely in collagen, elastin and glycoaminoglycans.
- DPPH diphenylpicrylhydrazyl
- a substance with an anti-radical action stabilises DPPH to a leuco-derivative (colourless).
- Iron complexed by ethylene diaminetetracetci acid forms hydroxyl radicals HO. in aqueous solution in contact with H 2 O 2 .
- a substance with an anti-radical action absorbs the HO. radicals, thus reducing the formation of red-coloured compound.
- the iron is complexed with EDTA in Halliwell's method and not complexed in Aruoma's method in order to evaluate the capacity for capture by the substance being evaluated (for example desferrioxamine inactivates Fenton's reaction by capturing iron).
- hydroxyl radicals are revealed by a sugar, deoxyribose (present in ADN); when HO. is present this forms a compound which reacts with thiobarbituric acid (TBA) in the form of a pink fluorescent condensate.
- TAA thiobarbituric acid
- An enzyme system (hypoxanthine/xanthine oxidase: XOD) forms radical superoxide anions O 2 . which react with luminol to form an intermediate compound; when this compound stabilises it has a luminescence which is recorded with a photoelectron multiplier.
- a substance with an anti-radical action absorbs or destroys the O 2 . anion and thus reduces the formation of luminescence.
- An enzyme system (hypoxanthine/xanthine oxidase: XOD) forms radical superoxide anions O 2 . which gradually dismute to H 2 O 2 and O 2 . H 2 O 2 and O 2 . react with microperoxidase (M) to form O 2 1 (singlet oxygen) which degrades luminol (Lu) to form a luminescent composition, the light emission from which is recorded with a photoelectron multiplier.
- M microperoxidase
- Li luminol
- An anti-radical substance absorbs either H 2 O 2 or O 2 . or O 2 1 and thus reduces the formation of luminescence.
- An enzyme system (hypoxanthine/xanthine oxidase) forms radical superoxide anions I 2 ..
- the O 2 . anions react with NBT to form a red compound, formazan.
- An anti-radical substance absorbs or destroys the O 2 . anion and thus reduces the formation of formazan.
- UV-A radiation is a factor which determines ageing of the skin, either through deterioration of the dermic matrix or through cellular damage.
- MDA malonal dialdehyde
- the MRC5 cells were recovered in dilute sodium hydroxide to measure the proteins (Bradford method) and glutathione (GSH) by means of a fluorescent probe (Hissin, 1976).
- GSH gives cytoplasm a reducing potential which is necessary for correct operation of the cellular mechanism; GSH also captures the reactive forms of oxygen and enables peroxide lipids to be regenerated by GSH peroxidase.
- UVB rays cause inflammation (erythema, oedema) by activating an enzyme, phospholipase A2.
- A431 keratinocytes are seeded in a defined medium with foetal calf serum.
- the keratinocytes are immediately irradiated with a dose of UVB (30 mJ/cm 2 ; tubes of the DUKE FL40E type), incubated for 24 hours at 37° C., then the content of PGE2 and of LDH in the supernatant medium is measured.
- the number of adhering keratinocytes is determined (after trypsination) by a particle counter and the content of PGE2 is determined by an ELISA test.
- LDH lactate-dehydrogenase/cytoplasmic enzyme
- Elastin is a dermic macromolecule organised as a structured network, which gives the skin its elastic properties.
- the elastic network is degraded with age, with more serious damage to the uncovered parts exposed to solar radiation, and the result is loss of elasticity and great flaccidity of the skin.
- the anti-collagenase in tubo action of an extract is measured with a collagenase of clostridium hystoliticium and a synthetic chromogenic substrate, FALGPA.
- Incubation is carried out for 30 mn at room temperature and the optical density is measured at 324 nm.
- Non-enzymatic glycation of proteins is a process which determines the ageing of human tissues; it explains the cross-linking of extra-cellular matrices and basal membranes which is largely responsible for the pathology observed in diabetics.
- Tests in tubo were carried out on type I collagen incubated for 21 days at 45° C. in the presence of 1% glucose, and the content of Schiff's bases is evaluated by densitometry at 350 nm and by fluorimetry at 430 nm (excitation at 350 nm).
- the metabolism-stimulating action brings an increase in oxygen consumption.
- Active ingredients which have this property participate in improved cell regeneration and may be used successfully for treating skins which are tired, stressed or exposed to pollution and for combatting the effect of ageing.
- Oxygen consumption is determined by polarographic measurements on a homogenate of epithelial cells using an oxygraph of the type produced by Gilson, fitted with a so-called Clark electrode.
- Oxygen consumption is recorded first under control conditions (epithelial cells in a buffer), then when the substance to be tested has been added to the medium, the final concentration of that substance being 1%.
- Oxygen consumption is calculated from the polarographic curves and the effect of the product is expressed as a percentage increase in oxygen consumption relative to the control conditions (in the absence of the active ingredient to be tested).
- the invention also concerns a composition or a cosmetic product for external topical use on the skin, mucous membrane and/or exoskeleton containing as active ingredient(s), particularly with a high-strength anti-radical action and a high-strength action in stimulating reduced glutathione auto-synthesis, alone or combined with at least one other active ingredient, at least one extract from a plant chosen from the group comprising Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus.
- a plant chosen from the group comprising Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus.
- the active ingredient is at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis and Gouania blanchetiana.
- the active ingredient is at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis and Waltheria indica.
- the cosmetic composition advantageously contains as an active ingredient, alone or combined with other active ingredients, between 0.001 and 20% by weight, preferably between 0.1 and 3% by weight, of a plant extract or mixture of plant extracts as defined above.
- a cosmetic product in the form of a bleaching cream for the skin according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
- Fraction A Glycerol stearate (and) 15% Clusareth 20 Paraffin oil 3.00% Ascorbyl palmitate 3.00% Dimethicone 3.00% Cetyl alcohol 0.50% PEG 30 - glycerol isostearate 2.00%
- Fraction B Water 72.20% Methylparaben 0.20% Imidazolidinyl urea 0.30%
- Fraction C Perfume 0.30%
- the method of preparing and producing the above-mentioned cream comprises melting the fraction A ingredients with agitation at 75° C., preparing fraction B at 75° C., then pouring fraction A into fraction B with agitation by a turbine, cooling with agitation by a planetary gear and adding fraction C.
- a cosmetic product in the form of an anti-stain emulsion for the hands according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
- the emulsion may also be a multiple-action product particularly with an anti-radical, anti-UVA and anti-UVB, anti-protease, anti-glycation and cellular metabolism-stimulating action.
- Fraction A Glycerol stearate (and) 6.00% Stearate PEG 100 Oleic alcohol 1.50% Glycerol stearate 2.00% 2-steareth 2.00% Shea butter 3.00% Dimethicone 4.00% Caprylic/capric triglyceride 8.00% Propylparaben 0.10% Tocopherol acetate 0.10%
- Fraction B Water 62.30% Elestab 388 2.50% (Laboratoires Sérobiologiques) Aqueous extract of Maprounea 1.00% guianensis (as in Example 2) Ethanol extract of Spondias 0.50% mombin (as in Example 1) Propylene glycol 5.00%
- Fraction C Polyacrylamide (and) isoparaffin 2.00% (and) Laureth 7
- the method of preparing and producing the above-mentioned emulsion comprises preparing fractions A and B separately at 75° C., adding fraction A to fraction B with agitation by a turbine at 75° C., then cooling the mixture obtained to 50° C. and adding fraction C, and lastly cooling the final mixture to room temperature.
- a cosmetic product in the form of an anti-stress, light-protective conditioner for dry hair according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A and B.
- Fraction A Cetyl alcohol 2.00% Liquid paraffin 2.00% Sorbitol stearate 1.00% Isopropyl palmitace (and) 1.00% castor oil
- Fraction B Glycerin 2.00% Laureth 20 1.00% Cetrimonium chloride 2.00%
- Ethanol extract of Spondias 0.50% mombin (as in Example 1) Aqueous extract of Randia armata 0.10% Elestab 388 1.50% (Laboratoires Sérobiologiques) Water sufficient to make up 100.00%
- the method of preparing and producing the above-mentioned conditioner comprises preparing fractions A and B separately with agitation at 80° C., adding fraction A to fraction B with agitation by a turbine and finally cooling the mixture obtained to room temperature.
- a cosmetic product in the form of an anti-stress protective body cream according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
- Fraction A Glycerol stearate (and) Cdisputedareth 20 6.00% (and) Ccludeareth 10 (and) ketostearylic alcohol (and) cetyl palmitate Ketostearylic alcohol 1.00% Decyl oleate 3.00% Liquid paraffin 4.00% Shea butter 2.00%
- Fraction B Glycerin 3.00% Hydrolysed wheat proteins 0.50% Ethanol extract of Hibiscus furcellatus 1.25% (as in Example 7) 1 Aqueous extract of Randia armata 0.60% (as in Example 6) Ethanol extract of Cordia schomburgkii 0.20% (as in Example 4) Water 78.25%
- Fraction C Perfume 0.20%
- the method of preparing and producing the above-mentioned emulsion comprises preparing fractions A and B separately with agitation at 80° C., adding fraction A to fraction B with agitation by a turbine, then bringing the mixture obtained back to room temperature and adding fraction C.
- a cosmetic product in the form of an anti-ageing, multiple-action (anti-radical, anti-UVA and anti-elastase) day cream according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
- Fraction A Glycerol stearate 14.00% Octyl dodecanol 6.00% Dibutyl adipate 6.00% Cigiareth 12 1.50% Ccieareth 20 1.50%
- Fraction B Propylene glycol 5.00% Ethanol extract of Maprounea guianensis 0.75% (as in Example 2) Aqueous extract of Waltheria indica 1.00% (as in Example 5) Ethanol extract of Randia armata 0.50% (as in Example 6) Elestab 4112 0.40% (Laboratoires Sérobiologiques) Water sufficient to make up 100.00%
- Fraction C Perfume 0.20%
- the method of preparing and producing the above-mentioned cream comprises preparing fractions A and B separately with agitation at 80° C., adding fraction A to fraction B with agitation by a turbine, cooling the mixture obtained to 45° C., then adding fraction C and lastly bringing the final mixture back to room temperature.
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Abstract
The invention concerns a cosmetic product containing plant extracts in particular with depigmentation, antioxidant and anti-ageing action. The invention also concerns the use as active agent for preparing a cosmetic product for topical application on the skin, the mucous membranes and/or superficial body growth, of a least a plant extract selected in the group formed by Spondias mombin, Maprounea guianensis, Walteria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus.
Description
- The invention concerns the cosmetics, more particularly the dermo-cosmetics field, and its subject is the use of plant extracts, found especially in Haut-Maroni (French Guiana), in the preparation of cosmetics for the skin, mucous membrane and/or exoskeleton (hair, nails, . . . ).
- The Aluku women (Haut-Maroni, French Guiana) traditionally carry out their ritual intimate ablutions every morning and evening with a plant decoction specific to each stage of a woman's life, using a large number of local plants.
- The plants are prepared by decoction then used in a hip bath according to the stage in he menstrual cycle and the required effect.
- A study of these various plants has revealed some specific pharmaceutical properties, namely antiseptic, healing, astringent and tonic properties (see “Végétaux utilisés pour l'hygiène intime des femmes Aluku en Guyane Francaise: interprétation culturelle et intérêt pharmacologique” (Plants used for intimate hygiene of Aluku women in French Guiana: cultural interpretation and pharmacological importance), Marie FLEURY, Proceedings of the 2nd European Colloquium on Ethno-pharmacology and the 11th international Conference on Ethno-medicine, Heidelberg, Mar. 24-27, 1993).
- The inventors of this invention have made the unexpected and surprising discovery that extracts of some of the above-mentioned plants have not only the fore-mentioned pharmacological properties but also clear anti-free-radical, anti-ageing and glutathione auto-synthesis reducing properties and action, which are at least equivalent—if not superior—to those of the active ingredients normally used in cosmetics.
- The main subject of the invention is thus the use, as an active ingredient for the preparation of a cosmetic for topical use on the skin, mucous membrane and/or exoskeleton, of at least one extract or mixture of extracts from a plant selected from the group made up ofSpondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus, particularly with an enhanced anti-radical and glutathione auto-synthesis reducing action.
- This action may in particular be advantageously used in anti-ageing, anti-physical-stress (UV-R), cold, heat, wind and anti-chemical stress (especially pollution) and light-protective cosmetics, in anti-stress and light-protective capillary preparations or in sun or after-sun products.
- The above-mentioned extracts may for example be prepared from the whole plant or plants.
- However the plant extracts are advantageously obtained from the aerial parts of the plant, for example the stems, leaves, flowers and/or buds.
- Once collected the plants or parts of the plants in question, posssibly after being dried, are subjected to an extraction process, preferably in two successive operations producing two different extracts. The extracting solvent used may advantageously be chosen from the group formed by water, aqueous solutions with different pH levels, C1-C6 alcohols, ketones (acetone, methylketone, diethylketone), halogenated hydrocarbons, esters (ethyl acetate, propyl actetate, butyl acetate or the like), polyhydric alcohols (glycols, diethylene glycol, propane diol, dipropylene glycol, butylene glycol) or mixtures of at least two of the fore-mentioned substances.
- In a special embodiment of the invention the plant extract(s) comprise one or more separated, purified fractions extracted from one or more of the plants.
- To obtain a cosmetic composition which also has an enhanced anti-elastase action, protecting and repairing the elastic fibres of the dermis better than the anti-ageing active ingredients currently used in cosmetics, the active ingredient incorporated in it is advantageously at least one plant extract selected from the group formed bySpondias mombin, Maprounea guianensis, Gouania blanchetiana, Waltheria indica and Cordia schomburgkii.
- To obtain a cosmetic composition which also has a strong anti-collagenase action, the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed by ofSpondias mombin, Maprounea guianensis, Waltheria indica and Randia armata.
- To obtain a cosmetic composition which also has a strong anti-UVA effect, the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed bySpondias mombin, Maprounea guianensis and Waltheria indica.
- To obtain a cosmetic composition which additionally has a strong anti-UVB effect, the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed bySpondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana and Randia armata.
- To obtain a cosmetic composition which additionally has an enhanced anti-tyrosinase action, inducing a better de-pigmenting effect than current de-pigmenting ingredients, the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed bySpondias mombin, Maprounea guianensis and Gouania blanchetiana.
- To obtain a cosmetic composition which also has a strong cellular metabolism stimulating action, the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed bySpondias mombin, Maprounea guianensis, Gouania blanchetiana, Cordia schomburgkii and Hibiscus furcellatus,
- To obtain a cosmetic composition which also has an enhanced anti-glycosylation action, the active ingredient incorporated in it is advantageously at least one plant extract chosen from the group formed bySpondias mombin, Maprounea gulanensis, Gouania blanchetiana and Cordia schomburgkii.
- In a preferred embodiment of the invention producing an active ingredient which simultaneously has a good anti-tyrosinase action, a very strong anti-protease action (anti-elastase and anti-collagenase), a very strong anti-UVA and anti-UVB action, a significant cellular metabolism stimulating action and a significant anti-glycosylation affect, it may be envisaged that the active ingredient will preferably be an extract or a mixture of extracts obtained from theSpondias mombin plant and/or the Maprounea guianensis plant.
- As a non-restrictive illustration, various methods which are preferably used to make the above-mentioned plant extracts will now be described:
- Thus a method of preparing an aqueous extract may for example comprise the following stages:
- suspending the ground plant material in 5 to 10 volumes of distilled water in a reactor;
- extracting for one hour at 85-90° C. with agitation;
- cooling to room temperature;
- centrifuging for 15 min at 5000 g or coarse filtering;
- clarifying if necessary on deep filters, up to 0.5 μm;
- noting the volume of extract E1 collected and determining its content of dry material;
- extracting and treating the moist residue under the same conditions to obtain an extract E2;
- dehydrating the two extracts by spraying the plant extract, possibly after including an additive such as maltodextrin (⅔ additive to ⅓ extracted material). [Inlet temperature: 185-190° C./outlet temperature: 75-80° C.]
- A method of preparing an aqueous alcohol extract may for example comprise the following stages:
- suspending the ground plant material in 5 to 10 volumes of aqueous ethanol in a reactor;
- extracting with agitation for one hour under reflux;
- filtering through a Buchner funnel fitted with a deep filter;
- collecting the supernatant material, evaporating the ethanol phase at reduced pressure, centrifuging if necessary for 10 min at 5000 g to eliminate insoluble material, and filtering;
- extracting and treating the moist residue under the same conditions to obtain an extract E2;
- dehydrating the two extracts by direct spraying of the plant extract, possibly after including an additive such as maltodextrin (⅔ additive to ⅓ extracted material).
- A method of preparing an alcohol extract may for example comprise the following stages:
- suspending the ground plant material in 5 to 10 volumes of ethanol in a reactor;
- extracting with agitation for one hour under reflux;
- cooling to room temperature;
- filtering through a Buchner funnel fitted with a deep filter;
- evaporating the alcohol at reduced pressure at 45° C.;
- drying in an oven at 40° C.;
- extracting and treating the moist residue under the same conditions to obtain an extract E2.
- By carrying out the preparation processes described above various extracts have been obtained from the plants in question, as mentioned in Examples 1 to 7 below.
- Dry leaves ofSpondias mombin were treated by the operating methods previously described, to obtain aqueous, alcoholic and aqueous alcohol extracts.
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) Aqueous ethanol) Ethanol Weight of material 200 200 200 treated (g) First Colour dark brown green extract brown E1 Extraction yield (%) 17.12 23.00 Dry weight of extract 26.02 33.90 37.35 after drying (g) True yield after drying (%) 13.01 16.95 18.68 Second Colour brown brown dark extract brown E2 Extraction yield (%) 7.29 7.60 Dry weight of extract 8.14 9.23 9.38 after drying (g) True yield after drying (%) 4.07 4.62 14.69 Total yield (%) 17.08 21.57 23.37 - It will be noted that the extraction yield corresponds to the extracted material present in the solution, that is to say
- and that the true yield after drying corresponds to the yield of material obtained in dry form and allows for losses during the drying process.
- Dry leaves ofMaprounea guianensis were treated by the operating methods previously described.
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) Aqueous ethanol) Ethanol Weight of material 200 200 200 treated (g) First Colour orange- brown brown extract brown E1 Extraction yield (%) 6.87 nd Dry weight of extract 7.00 36.65 24.67 after drying (g) True yield after drying (%) 3.50 18.33 12.34 Second Colour orange- brown dark extract brown brown E2 Extraction yield (%) 13.25 nd Dry weight of extract 15.04 25.14 13.30 after drying (g) True yield after drying (%) 7.52 12.57 6.65 Total yield (%) 11.02 30.90 18.99 - Dry leaves ofGouania blanchetiana were treated by the operating methods previously described.
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) Aqueous ethanol) Ethanol Weight of material 200 200 200 treated (g) First Colour brown brown green extract Extraction yield (%) 6.10 11.86 E1 Dry weight of extract 8.90 13.28 13.87 after drying (g) True yield after drying (%) 14.45 6.64 6.94 Second Colour brown brown dark extract brown E2 Extraction yield (%) 5.05 5.00 Dry weight of extract 5.07 14.67 5.22 after drying (g) True yield after drying (%) 2.54 2.34 2.61 Total yield (%) 6.99 8.98 9.55 - Dry leaves ofCordia schomburgkii were treated by the operating methods previously described to obtain alcoholic and aqueous alcohol extracts.
- In this example extracts E1 and E2 of the alcoholic extract were combined before drying: (for aqueous alcohol extraction an additive is included before spraying: the corresponding weights are the weights of dry material not counting the weight of additive).
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) ethanol) Ethanol Weight of material 200 200 treated (g) First Colour green green extract Extraction yield (%) 14.60 E1 Dry weight of extract 20.81 17.16 after drying (g) True yield after drying (%) 10.41 8.58 Second Colour green extract Extraction yield (%) E2 Dry weight of extract 10.13 after drying (g) True yield after drying (%) 5.07 Total yield (%) 13.65 - A mixture of leaves and dry buds ofWaltheria indica was treated by the operating methods previously described to obtain aqueous, alcoholic and aqueous alcohol extracts.
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) Aqueous ethanol) Ethanol Weight of material 150 150 150 treated (g) First Colour brown brown green extract Extraction yield (%) 9.83 9.82 E1 Dry weight of extract 7.27 7.05 10.31 after drying (g) True yield after drying (%) 4.85 4.70 6.87 Second Colour brown brown green extract Extraction yield (%) 15.05 1.70 E2 Dry weight of extract 4.03 2.16 3.06 after drying (g) True yield after drying (%) 2.69 1.44 2.04 Total yield (%) 7.53 6.14 8.91 - Dry leaves ofRandia armata were treated by the operating methods previously described to obtain aqueous, alcoholic and aqueous alcohol extracts.
- In this example extracts E1 and E2 were combined before drying: (for aqueous and aqueous alcohol extraction an additive is included before spraying: the corresponding weights are the weights of extracted material not counting the weight of additive).
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) Aqueous ethanol) Ethanol Weight of material 200 200 200 treated (g) First Colour light brown green extract brown E1 Extraction yield (%) 25.50 36.50 Dry weight of extract 34.00 59.75 51.38 after drying (g) True yield after drying (%) 17.00 29.88 25.69 Second Colour green extract Extraction yield (%) E2 Dry weight of extract 15.15 after drying (g) True yield after drying (%) 7.58 Total yield (%) 33.27 - Dry leaf-bearing stems ofHibiscus furcellatus were treated by the operating methods previously described to obtain alcoholic and aqueous alcohol extracts.
- In this example extracts E1 and E2 of the aqueous alcohol extract were combined before drying: (for aqueous alcohol extraction an additive is included before spraying: the corresponding weights are the weights of extracted material not counting the weight of additive).
- The following results were obtained:
Aqueous alcohol (50% Extract (properties) ethanol) Ethanol Weight of material 150 150 treated (g) First Colour brownish green extract green E1 Extraction yield (%) 8.99 Dry weight or extract 7.96 3.78 after drying (g) True yield after drying (%) 5.31 2.52 Second Colour dark extract brown E2 Extraction yield (%) Dry weight of extract 1.22 after drying (g) True yield after drying (%) 0.81 Total yield (%) 3.33 - The cosmetically important properties and action of the above-mentioned plant extracts, discovered by the inventors, have been demonstrated and quantitatively evaluated by means of various tests known in the art, which are briefly explained below and the results of which are set out in Table I.
- 1) Evidence of a De-pigmenting Action
- To determine the de-pigmenting power of the extracts, their tyrosinase action was evaluated by measuring the product of oxidising L-DOPA to DOPACHROME (by HS Mason's method, 1948), carrying out the following stages:
- mixing tyrosinase with L-DOPA in the phosphate buffer with an automatic pipette
- recording the optical density at 475 nm for 2 minutes, then
- calculating the initial speed of the enzyme reaction and
- determining the C150 (concentration which halves the initial speed of DOPACHROME formation).
- Referring to Table I above, it will be noted that the results surprisingly and unexpectedly show a clear anti-tyrosinase effect of the plant extracts, comparable or even superior to that of the reference substance (hydroquinone).
- 2) Evidence of an Anti-radical Action
- Aggressive action by free radicals is one of the main skin-damaging mechanisms owing to the action of stress factors in the external environment.
- Free radicals, particularly the reactive forms of oxygen (superoxide radical O2., hydroxyl radical HO., singlet oxygen O2 1, hydrogen peroxide H2O2), act on a cellular scale: particularly damaging the membranous lipids with the formation of lipoperoxides, deterioration of proteins, blocking of enzyme systems and deterioration of the nuclear genetic capital. Noxious effects are also produced in the macromolecules of the extra-cellular matrix of the dermis, namely in collagen, elastin and glycoaminoglycans.
- An equally surprising and unexpected observation was the powerful anti-radical action of the various extracts obtained (see Table I below)
- This action was evaluated by the following chemical and biochemical tests in tubo:
- a) Anti-free-radical Action: DPPH Test (C Deby, 1970)
- DPPH (diphenylpicrylhydrazyl) is a stable radical which forms a violet-coloured solution when dissolved in a methanol.
- A substance with an anti-radical action stabilises DPPH to a leuco-derivative (colourless).
- b) Anti-HO. Action: Salicylic Acid Method (M Tien, 1982)
- Iron complexed by ethylene diaminetetracetci acid forms hydroxyl radicals HO. in aqueous solution in contact with H2O2.
- The hydroxyl radicals react with salicylic acid (S.A.) to form a reddish-coloured compound.
- A substance with an anti-radical action absorbs the HO. radicals, thus reducing the formation of red-coloured compound.
- c) Anti-HO. Action: Deoxyribose Method (Halliwell's Method, 1987 or Aruoma's Method, 1988)
- The hydroxyl radicals HO. are produced by Fenton's reaction:
- Fe+++H2O2→Fe++++HO.+OH−.
- The iron is complexed with EDTA in Halliwell's method and not complexed in Aruoma's method in order to evaluate the capacity for capture by the substance being evaluated (for example desferrioxamine inactivates Fenton's reaction by capturing iron).
- The hydroxyl radicals are revealed by a sugar, deoxyribose (present in ADN); when HO. is present this forms a compound which reacts with thiobarbituric acid (TBA) in the form of a pink fluorescent condensate.
- d) Anti-O2. Action: Luminol Method (G M Oyamburo, 1970)
- An enzyme system (hypoxanthine/xanthine oxidase: XOD) forms radical superoxide anions O2. which react with luminol to form an intermediate compound; when this compound stabilises it has a luminescence which is recorded with a photoelectron multiplier.
- A substance with an anti-radical action absorbs or destroys the O2. anion and thus reduces the formation of luminescence.
- e) Anti-O2 and H2O2 Action: Luminol Microperoxidase Method (M Israel, 1985)
- An enzyme system (hypoxanthine/xanthine oxidase: XOD) forms radical superoxide anions O2. which gradually dismute to H2O2 and O2. H2O2 and O2. react with microperoxidase (M) to form O2 1 (singlet oxygen) which degrades luminol (Lu) to form a luminescent composition, the light emission from which is recorded with a photoelectron multiplier.
- An anti-radical substance absorbs either H2O2 or O2. or O2 1 and thus reduces the formation of luminescence.
- f) Anti-O2. Action: Blue Neotetrazolium (NBT) Method (N Ohkuma, 1987)
- An enzyme system (hypoxanthine/xanthine oxidase) forms radical superoxide anions I2..
- The O2. anions react with NBT to form a red compound, formazan. An anti-radical substance absorbs or destroys the O2. anion and thus reduces the formation of formazan.
- 3) Evidence of an Anti-UV-A Action
- UV-A radiation is a factor which determines ageing of the skin, either through deterioration of the dermic matrix or through cellular damage.
- The surprising and unexpected observation has been made that the various plant extracts obtained are clearly active in protecting cells from UV-A.
- Human fibroblasts of the MRC5 type were cultivated in a growth medium until the tapetum cellulosum was saturated.
- The growth medium was then replaced with a standard medium (DMEM+SVF, 1%) containing each extract in the various quantities to be tested (see Table I).
- After incubating for 48 hours at 37° C. the various media were replaced with a saline solution (Hanks), then the MRC5 were irradiated on top with fluorescent tubes.
- As soon as the irradiation was over the saline solution was removed to measure the malonal dialdehyde (MDA) by reaction with warm thiobarbituric acid (fluorescence at 560 nm). MDA is a degradation product of the unsaturated lipids which make up biological membranes. It causes bridges which inhibit enzymes and form lipofuscins (ageing marks), and would be mutagenic.
- The MRC5 cells were recovered in dilute sodium hydroxide to measure the proteins (Bradford method) and glutathione (GSH) by means of a fluorescent probe (Hissin, 1976). GSH gives cytoplasm a reducing potential which is necessary for correct operation of the cellular mechanism; GSH also captures the reactive forms of oxygen and enables peroxide lipids to be regenerated by GSH peroxidase.
- 4) Evidence of the Anti-UVB Cell-protecting Effect on Human Keratinocytes Surviving in vitro
- UVB rays cause inflammation (erythema, oedema) by activating an enzyme, phospholipase A2.
- This enzyme releases arachidonic acid from the phospholipids of the plasma membrane: arachidonic acid is the precursor of prostaglandins, which are mediators of inflammation, and E2 prostaglandins (=PGE2) are thus formed by cyclooxygenase.
- A431 keratinocytes are seeded in a defined medium with foetal calf serum.
- After 72 hours at 37° C. with CO2=5% the culture medium is replaced by a saline solution containing the plant extract to be tested.
- The keratinocytes are immediately irradiated with a dose of UVB (30 mJ/cm2; tubes of the DUKE FL40E type), incubated for 24 hours at 37° C., then the content of PGE2 and of LDH in the supernatant medium is measured.
- The number of adhering keratinocytes is determined (after trypsination) by a particle counter and the content of PGE2 is determined by an ELISA test.
- The content of LDH (lactate-dehydrogenase/cytoplasmic enzyme), which is a marker of cellular strain, is also determined by an enzyme reaction.
- The action of the plant extract tested is expressed as a % variation of the two markers relative to the values obtained with the control substances (average of 2 tests, each in duplicate—see Table I).
- 5) Evidence of an Anti-ageing Action
- Elastin is a dermic macromolecule organised as a structured network, which gives the skin its elastic properties.
- The elastic network is degraded with age, with more serious damage to the uncovered parts exposed to solar radiation, and the result is loss of elasticity and great flaccidity of the skin.
- One of the mechanisms of this process is enzyme hyperactivity in the digestion of elastic fibres by endogenous elastases, and the beneficial effect of elastase inhibitors as an active anti-ageing substance is well known.
- The surprising and unexpected observation has been made that the extracts obtained have a clear anti-elastase action (cf. Table I).
- The method used to demonstrate the inhibition of elastase is the method with synthetic substrate, chiefly comprising the following stages:
- use of 1 mM N-succinyl-(Ala.)3-para-Nitroanilide in a TRIS buffer (pH=8);
- incubation of the substance with elastase and the substrate for 20 minutes at 20° C.;
- appearance of yellow colouring, which is measured by spectrophotometry at 410 nm.
- 6) Evidence of an Anti-collagenase Action in tubo (Van Wart's Method, 1981)
- Proteases secreted by polymorphonuclear neutrophilic leukocytes when there is inflammation or by fibroblasts subjected to irradiation by UVA cause degradation of the proteins which structure the extracellular matrix of the dermis.
- The anti-collagenase in tubo action of an extract is measured with a collagenase of clostridium hystoliticium and a synthetic chromogenic substrate, FALGPA.
- Incubation is carried out for 30 mn at room temperature and the optical density is measured at 324 nm.
- The results are expressed as % inhibition of the enzyme relative to the control substance without active ingredient (Table I).
- 7) Evidence of Action Inhibiting Non-enzymatic Glycation in tubo (Devi's “Anti-glycation” Action, 1990 and Monnier's, 1984)
- Non-enzymatic glycation of proteins is a process which determines the ageing of human tissues; it explains the cross-linking of extra-cellular matrices and basal membranes which is largely responsible for the pathology observed in diabetics.
- In addition, Schiff's bases catalyse the production of reactive forms of oxygen which aggravate the effects of non-enzymatic glycation.
- Tests in tubo were carried out on type I collagen incubated for 21 days at 45° C. in the presence of 1% glucose, and the content of Schiff's bases is evaluated by densitometry at 350 nm and by fluorimetry at 430 nm (excitation at 350 nm).
- The results are expressed as % Inhibition of the formation of Schiff's bases relative to the control specimens (Table I).
- 8) Evidence of Stimulation of Cellular Metabolism
- The metabolism-stimulating action brings an increase in oxygen consumption. Active ingredients which have this property participate in improved cell regeneration and may be used successfully for treating skins which are tired, stressed or exposed to pollution and for combatting the effect of ageing.
- Oxygen consumption is determined by polarographic measurements on a homogenate of epithelial cells using an oxygraph of the type produced by Gilson, fitted with a so-called Clark electrode.
- Oxygen consumption is recorded first under control conditions (epithelial cells in a buffer), then when the substance to be tested has been added to the medium, the final concentration of that substance being 1%.
- Oxygen consumption is calculated from the polarographic curves and the effect of the product is expressed as a percentage increase in oxygen consumption relative to the control conditions (in the absence of the active ingredient to be tested).
- The results correspond to the mean +/− SEM of 3 tests (Table I).
- As shown in Table I below, which contains the results of the 8 tests described above, all the plant extracts obtained also have a marked anti-radical action and a marked action in stimulating reduced glutathione auto-synthesis, for use n anti-ageing cosmetics, and cosmetics to counter physical stresses (UV-R, cold, heat, wind) or chemical stresses (for example pollution).
TABLE 1 Anti-UV-A Anti UV-B Anti-free-radical effect in % inhibition effect on effect on C150 anti- (0 03 or 0.1% doses) MRC 5 A431 tyrosinase Tests %/control %/control effect in DPPH Fenton reaction Lu. + 0.001 to 0.006% doses Extract Plant % test A.S. Desoxyribose Lu M NBT MDA GSH PGE2 LDH Ethanolic Spondias 0.017 92 40 46 71 100 97 68 61 30 −73 −88 extract mombin example 1 Aqueous Maprounea 0 015 92 35 55 76 100 99 74 34 38 −99 −100 extract guianensis example 2 Ethanolic Maprounea 0 015 91 47 32 79 100 99 70 53 45 −100 −100 extract guianensis example 2 50% Gouania 0 015 88 0 27 67 100 91 75 −13 14 −92 −100 ethanol blanche- extract tiana example 3 Aqueous Waltheria 0 08 88 16 8 47 100 96 62 −43 71 −25 −70 extract indica example 5 70% Cordia 0 09 89 30 3 19 99 97 31 −25 10 ethanol scomburgkii extract example 4 Aqueous Randia 0 18 92 12 0 34 100 96 40 −15 37 20 −70 extract armata example 6 50% Randia 0 13 92 18 0 42 100 96 38 −11 43 −70 −89 ethanol armata extract example 6 50% Hibiscus 0.83 86 19 0 14 99 42 10 0 42 −2 −17 ethanol furcellatus extract example 7 Hydro- 0 025 quinone Anti- glycation Stimulation effect % of Anti- Anti- inhibition metabolism elastase colagenase of Schiff's % increase effect at effect at base in O2 0.3% 0.3% formation/ consumption Inhibition Inhibition 0.01 to (m+/−SEM) Extract Plant in % in % 0.1% doses 1% doses Ethanolic Spondias 87 99 43 93 +/− 24 extract mombin example 1 Aqueous Maprounea 80 8 100 63 +/− 7 extract guianensis example 2 Ethanolic Maprounea 85 100 61 +/− 20 extract guianensis example 2 50% Gouania 56 39 68 151 +/− 48 ethanol blanche- extract tiana example 3 Aqueous Waitheria 48 83 3 57 +/− 29 extract indica example 5 70% Cordia 37 17 54 79 +/− 17 ethanol scomburgkii extract example 4 Aqueous Randia 16 97 11 −54 extract armata example 6 50% Randia 17 99 −41 ethanol armata extract example 6 50% Hibiscus 17 13 0 118 +/− 31 ethanol furcellarus extract example 7 - The invention also concerns a composition or a cosmetic product for external topical use on the skin, mucous membrane and/or exoskeleton containing as active ingredient(s), particularly with a high-strength anti-radical action and a high-strength action in stimulating reduced glutathione auto-synthesis, alone or combined with at least one other active ingredient, at least one extract from a plant chosen from the group comprisingSpondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus.
- In a first preferred embodiment of the invention and with a view to obtaining a cosmetic composition which concurrently has a strong anti-tyrosinase action, anti-elastase action, anti-UVB action and anti-glycation action in addition to the advantageous properties mentioned above, the active ingredient is at least one plant extract chosen from the group formed bySpondias mombin, Maprounea guianensis and Gouania blanchetiana.
- In a second preferred embodiment of the invention and with a view to obtaining a cosmetic composition which concurrently has a high-strength anti-protease (anti-elastase and anti-collagenase) action and strong anti-UVA and anti-UVB effects in addition to the advantageous properties mentioned above, the active ingredient is at least one plant extract chosen from the group formed bySpondias mombin, Maprounea guianensis and Waltheria indica.
- The cosmetic composition advantageously contains as an active ingredient, alone or combined with other active ingredients, between 0.001 and 20% by weight, preferably between 0.1 and 3% by weight, of a plant extract or mixture of plant extracts as defined above.
- As non-restrictive examples of embodiments of the invention various cosmetic products or preparations containing a plant extract or mixture of plant extracts as previously described will now be described.
- A cosmetic product in the form of a bleaching cream for the skin according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
Fraction A: Glycerol stearate (and) 15% Cétéareth 20 Paraffin oil 3.00% Ascorbyl palmitate 3.00% Dimethicone 3.00% Cetyl alcohol 0.50% PEG 30 - glycerol isostearate 2.00% Fraction B: Water 72.20% Methylparaben 0.20% Imidazolidinyl urea 0.30% Ethanol extract of Gouania 0.50% blanchetiana (as in Example 3) Fraction C: Perfume 0.30% - The method of preparing and producing the above-mentioned cream comprises melting the fraction A ingredients with agitation at 75° C., preparing fraction B at 75° C., then pouring fraction A into fraction B with agitation by a turbine, cooling with agitation by a planetary gear and adding fraction C.
- A cosmetic product in the form of an anti-stain emulsion for the hands according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
- In accordance with the invention the emulsion may also be a multiple-action product particularly with an anti-radical, anti-UVA and anti-UVB, anti-protease, anti-glycation and cellular metabolism-stimulating action.
Fraction A: Glycerol stearate (and) 6.00% Stearate PEG 100 Oleic alcohol 1.50% Glycerol stearate 2.00% 2-steareth 2.00% Shea butter 3.00% Dimethicone 4.00% Caprylic/capric triglyceride 8.00% Propylparaben 0.10% Tocopherol acetate 0.10% Fraction B: Water 62.30% Elestab 388 2.50% (Laboratoires Sérobiologiques) Aqueous extract of Maprounea 1.00% guianensis (as in Example 2) Ethanol extract of Spondias 0.50% mombin (as in Example 1) Propylene glycol 5.00% Fraction C: Polyacrylamide (and) isoparaffin 2.00% (and) Laureth 7 - The method of preparing and producing the above-mentioned emulsion comprises preparing fractions A and B separately at 75° C., adding fraction A to fraction B with agitation by a turbine at 75° C., then cooling the mixture obtained to 50° C. and adding fraction C, and lastly cooling the final mixture to room temperature.
- A cosmetic product in the form of an anti-stress, light-protective conditioner for dry hair according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A and B.
Fraction A: Cetyl alcohol 2.00% Liquid paraffin 2.00% Sorbitol stearate 1.00% Isopropyl palmitace (and) 1.00% castor oil Fraction B: Glycerin 2.00% Laureth 20 1.00% Cetrimonium chloride 2.00% Ethanol extract of Spondias 0.50% mombin (as in Example 1) Aqueous extract of Randia armata 0.10% Elestab 388 1.50% (Laboratoires Sérobiologiques) Water sufficient to make up 100.00% - The method of preparing and producing the above-mentioned conditioner comprises preparing fractions A and B separately with agitation at 80° C., adding fraction A to fraction B with agitation by a turbine and finally cooling the mixture obtained to room temperature.
- A cosmetic product in the form of an anti-stress protective body cream according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
Fraction A: Glycerol stearate (and) Cétéareth 20 6.00% (and) Cétéareth 10 (and) ketostearylic alcohol (and) cetyl palmitate Ketostearylic alcohol 1.00% Decyl oleate 3.00% Liquid paraffin 4.00% Shea butter 2.00% Fraction B: Glycerin 3.00% Hydrolysed wheat proteins 0.50% Ethanol extract of Hibiscus furcellatus 1.25% (as in Example 7)1 Aqueous extract of Randia armata 0.60% (as in Example 6) Ethanol extract of Cordia schomburgkii 0.20% (as in Example 4) Water 78.25% Fraction C: Perfume 0.20% - The method of preparing and producing the above-mentioned emulsion comprises preparing fractions A and B separately with agitation at 80° C., adding fraction A to fraction B with agitation by a turbine, then bringing the mixture obtained back to room temperature and adding fraction C.
- A cosmetic product in the form of an anti-ageing, multiple-action (anti-radical, anti-UVA and anti-elastase) day cream according to the invention may for example have a composition by weight as indicated below, made up of the following fractions A, B and C.
Fraction A: Glycerol stearate 14.00% Octyl dodecanol 6.00% Dibutyl adipate 6.00% Cétéareth 12 1.50% Cétéareth 20 1.50% Fraction B: Propylene glycol 5.00% Ethanol extract of Maprounea guianensis 0.75% (as in Example 2) Aqueous extract of Waltheria indica 1.00% (as in Example 5) Ethanol extract of Randia armata 0.50% (as in Example 6) Elestab 4112 0.40% (Laboratoires Sérobiologiques) Water sufficient to make up 100.00% Fraction C: Perfume 0.20% - The method of preparing and producing the above-mentioned cream comprises preparing fractions A and B separately with agitation at 80° C., adding fraction A to fraction B with agitation by a turbine, cooling the mixture obtained to 45° C., then adding fraction C and lastly bringing the final mixture back to room temperature.
- The invention is not of course limited to the embodiments described. Modifications are still possible, particularly in respect of the constitution of the various components or substitution of equivalent techniques, without going beyond the scope of protection of the invention.
Claims (16)
1. Use of at least one extract of a plant chosen from the group formed by Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus, as an active ingredient in the preparation of a cosmetic product for topical use on the skin, mucous membrane and/or exoskeleton, with an anti-radical action and an high-strength action in stimulating reduced glutathione auto-synthesis.
2. Use according to claim 1 , characterised in that the plant extract or extracts are obtained from the aerial parts of the plants.
3. Use according to either of claims 1 or 2, characterised in that the solvent used for effecting the extraction is chosen from the group formed by water, aqueous solutions with different pH levels, C1-C6 alcohols, ketones, halogenated hydrocarbons, esters, polyhydric alcohols and mixtures of at least two of the fore-mentioned substances.
4. Use according to any of claims 1 to 3 , characterised in that the plant extract or extracts comprise one or more purified, separated fractions extracted from one or more of said plants.
5. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also has a high-strength anti-elastase action, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Gouania blanchetiana, Waltheria indica and Cordia schomburgkii.
6. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also has a high-strength anti-collagenase action, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Waltheria indica and Randia armata.
7. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also produces a strong anti-UVA effect, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis and Waltheria indica.
8. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also has a strong anti-UVB effect, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana and Randia armata.
9. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also has a high-strength anti-tyrosinase effect, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Gouania blanchetiana and Waltheria indica.
10. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also has a strong cellular metabolism-stimulating action, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Gouania blanchetiana, Cordia schomburgkii and Hibiscus furcellatus.
11. Use according to any of claims 1 to 4 , characterised in that the active ingredient, which also has a high-strength anti-glycation action, comprises at least one plant extract chosen from the group formed by Spondias mombin, Maprounea guianensis, Gouania blanchetiana and Cordia schomburgkii.
12. Use according to any of claims 1 to 11 , characterised in that the active ingredient comprises an extract or a mixture of extracts obtained from the Spondias mombin plant and/or the Maprounea guianensis plant.
13. A cosmetic composition for topical use on the skin, mucous membrane or exoskeleton, characterised in that it includes at least one extract from a plant chosen from the group formed by Spondias mombin, Maprounea guianensis, Waltheria indica, Gouania blanchetiana, Cordia schomburgkii, Randia armata and Hibiscus furcellatus, as an active ingredient with an anti-radical action and a high-strength action stimulating reduced glutathione auto-synthesis, either alone or combined with at least one other active ingredient.
14. A cosmetic composition according to claim 13 , characterised in that it includes an active ingredient which in particular has a strong anti-tyrosinase, anti-elastase, anti-UVB and anti-glycation action and comprises at least one extract from a plant chosen from the group formed by Spondias mombin, Maprounea guianensis and Gouania blanchetiana.
15. A cosmetic composition according to claim 13 , characterised in that it includes an active ingredient which has a high-strength anti-protease, particularly anti-elastase and anti-collagenase action and strong anti-UVA and anti-UVB effects, and comprises at least one extract from a plant chosen from the group formed by Spondias mombin, Maprounea guianensis and Waltheria indica.
16. A cosmetic composition for topical use on the skin, particularly with a high-strength anti-radical action and a high-strength action stimulating reduced glutathione auto-synthesis, characterised in that it contains from 0.001% to 20% by weight, preferably from 0.1% to 3% by weight of a plant extract or mixture of plant extracts according to any of claims 13 to 15 as an active ingredient, either alone or combined with other active ingredients.
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US6406720B1 US6406720B1 (en) | 2002-06-18 |
US20020076450A1 true US20020076450A1 (en) | 2002-06-20 |
Family
ID=9507834
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/455,402 Expired - Fee Related US6406720B1 (en) | 1997-06-06 | 1999-12-06 | Cosmetic containing plant extracts |
US10/143,871 Abandoned US20030072820A1 (en) | 1997-06-06 | 2002-05-14 | Cosmetic containing plant extracts |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/143,871 Abandoned US20030072820A1 (en) | 1997-06-06 | 2002-05-14 | Cosmetic containing plant extracts |
Country Status (10)
Country | Link |
---|---|
US (2) | US6406720B1 (en) |
EP (1) | EP0986370B1 (en) |
JP (1) | JP2002503242A (en) |
KR (1) | KR20010013361A (en) |
AT (1) | ATE275387T1 (en) |
AU (1) | AU7924098A (en) |
CA (1) | CA2291289A1 (en) |
DE (1) | DE69826122D1 (en) |
FR (1) | FR2764190B1 (en) |
WO (1) | WO1998055087A1 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050025737A1 (en) * | 2003-07-30 | 2005-02-03 | Sebagh Jean Louis | Compositions containing melon extracts |
US20090110651A1 (en) * | 2006-03-23 | 2009-04-30 | Cognis Ip Management Gmbh | Cosmetic Composition Comprising A Combination of a Sugar Fatty Acid Ester with a Plant Extract of Waltheria Indica or Pisum Sativum for Skin Whitening |
US20100203175A1 (en) * | 2007-07-09 | 2010-08-12 | Basf Beauty Care Solutions France S.A.S. | DEGLYCATION OF AGEs |
US7879369B2 (en) | 2007-09-18 | 2011-02-01 | Selvamedica, Llc | Combretum laurifolium Mart. extract and methods of extracting and using such extract |
EP3995128A4 (en) * | 2019-07-04 | 2023-08-09 | Natura Cosméticos S.A. | Method for obtaining bioactive ingredients, use of subcritical-water extraction process, bioactive ingredient, use of bioactive ingredient and cosmetic composition |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1121926A1 (en) * | 2000-02-07 | 2001-08-08 | Laboratoires Serobiologiques(Societe Anonyme) | Cosmetic preparations containing Waltheria indica extracts |
AU2005258148B2 (en) * | 2004-06-21 | 2008-03-20 | Aveda Corporation | Solvent based plant extracts |
DE102005013380A1 (en) * | 2005-03-23 | 2006-10-05 | Merck Patent Gmbh | extraction process |
US20090011060A1 (en) * | 2007-07-06 | 2009-01-08 | Peter Koepke | Campsiandra angustifolia extract and methods of extracting and using such extract |
US20090017140A1 (en) * | 2007-07-09 | 2009-01-15 | Peter Koepke | Maytenus abenfolia extract and methods of extracting and using such extract |
US20090035395A1 (en) * | 2007-08-01 | 2009-02-05 | Peter Koepke | Spondias mombin l. extract and methods of extracting and using such extract |
FR2928548B1 (en) * | 2008-03-14 | 2015-07-03 | Basf Beauty Care Solutions F | SUBSTANCES INCREASING THRESHOLD OF ACTIVATION OF IMMUNE CELLS |
EP2280718B1 (en) * | 2008-04-01 | 2016-07-27 | Lucas Meyer Cosmetics Canada Inc. | Extracts from plants of the tsuga genus and uses thereof in the treatment of inflammation, irritation and/or infection |
JP4638963B2 (en) * | 2008-04-07 | 2011-02-23 | 敏 新川 | Slimming cosmetic composition |
JP4993391B2 (en) * | 2009-06-17 | 2012-08-08 | 敏 新川 | Slimming application agent for slimming method |
WO2012033634A2 (en) | 2010-09-09 | 2012-03-15 | Mary Kay Inc. | Topical skin care formulations comprising plant extracts |
BR112016022735B1 (en) | 2014-04-30 | 2020-12-01 | Kimberly-Clark Worldwide, Inc. | method to reduce the signs of skin aging, method to increase the adipogenesis of the face and body and method to increase the lipogenesis of the face and body |
AU2014392686B2 (en) * | 2014-04-30 | 2019-08-22 | Kimberly-Clark Worldwide, Inc. | Topical composition including a combination of extracts for reducing signs of skin aging |
-
1997
- 1997-06-06 FR FR9707207A patent/FR2764190B1/en not_active Expired - Fee Related
-
1998
- 1998-06-05 JP JP50175799A patent/JP2002503242A/en active Pending
- 1998-06-05 AU AU79240/98A patent/AU7924098A/en not_active Abandoned
- 1998-06-05 KR KR19997011357A patent/KR20010013361A/en not_active Application Discontinuation
- 1998-06-05 EP EP98929508A patent/EP0986370B1/en not_active Expired - Lifetime
- 1998-06-05 CA CA002291289A patent/CA2291289A1/en not_active Abandoned
- 1998-06-05 WO PCT/FR1998/001159 patent/WO1998055087A1/en not_active Application Discontinuation
- 1998-06-05 DE DE69826122T patent/DE69826122D1/en not_active Expired - Lifetime
- 1998-06-05 AT AT98929508T patent/ATE275387T1/en not_active IP Right Cessation
-
1999
- 1999-12-06 US US09/455,402 patent/US6406720B1/en not_active Expired - Fee Related
-
2002
- 2002-05-14 US US10/143,871 patent/US20030072820A1/en not_active Abandoned
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050025737A1 (en) * | 2003-07-30 | 2005-02-03 | Sebagh Jean Louis | Compositions containing melon extracts |
US20090110651A1 (en) * | 2006-03-23 | 2009-04-30 | Cognis Ip Management Gmbh | Cosmetic Composition Comprising A Combination of a Sugar Fatty Acid Ester with a Plant Extract of Waltheria Indica or Pisum Sativum for Skin Whitening |
US9949917B2 (en) * | 2006-03-23 | 2018-04-24 | Cognis Ip Management Gmbh | Cosmetic composition comprising a combination of a sugar fatty acid ester with a plant extract of Waltheria indica or Pisum sativum for skin whitening |
US20100203175A1 (en) * | 2007-07-09 | 2010-08-12 | Basf Beauty Care Solutions France S.A.S. | DEGLYCATION OF AGEs |
US7879369B2 (en) | 2007-09-18 | 2011-02-01 | Selvamedica, Llc | Combretum laurifolium Mart. extract and methods of extracting and using such extract |
EP3995128A4 (en) * | 2019-07-04 | 2023-08-09 | Natura Cosméticos S.A. | Method for obtaining bioactive ingredients, use of subcritical-water extraction process, bioactive ingredient, use of bioactive ingredient and cosmetic composition |
Also Published As
Publication number | Publication date |
---|---|
KR20010013361A (en) | 2001-02-26 |
CA2291289A1 (en) | 1998-12-10 |
DE69826122D1 (en) | 2004-10-14 |
FR2764190B1 (en) | 1999-10-15 |
JP2002503242A (en) | 2002-01-29 |
ATE275387T1 (en) | 2004-09-15 |
US20030072820A1 (en) | 2003-04-17 |
EP0986370B1 (en) | 2004-09-08 |
US6406720B1 (en) | 2002-06-18 |
FR2764190A1 (en) | 1998-12-11 |
AU7924098A (en) | 1998-12-21 |
WO1998055087A1 (en) | 1998-12-10 |
EP0986370A1 (en) | 2000-03-22 |
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