US11998016B2 - Use of Chaetomium globosum strain in controlling fusarium crown rot of wheat - Google Patents

Use of Chaetomium globosum strain in controlling fusarium crown rot of wheat Download PDF

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US11998016B2
US11998016B2 US17/233,732 US202117233732A US11998016B2 US 11998016 B2 US11998016 B2 US 11998016B2 US 202117233732 A US202117233732 A US 202117233732A US 11998016 B2 US11998016 B2 US 11998016B2
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wheat
chaetomium globosum
globosum strain
fcr
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US20220053771A1 (en
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Fei Xu
Yuli Song
Junmei Wang
Jiaojiao ZHANG
Zixing HAN
Yahong Li
Ruijie Shi
Lijuan Li
Lulu Liu
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Institute of Plant Protection of Henan Academy of Agricultural Sciences
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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12R2001/645—Fungi ; Processes using fungi

Definitions

  • the present disclosure relates to use of a Chaetomium globosum strain in controlling Fusarium crown rot (FCR) of wheat.
  • Fusarium crown rot an important root and crown rot of wheat, can reduce grain weight per ear and 1,000-grain weight.
  • the disease can infect at the seedling, tillering, jointing and adult stages of wheat.
  • Typical symptoms include the browning of the base of the wheat stem to appear the color of soy sauce, and scattered white ears appeared in the field during the filling stage.
  • Such microorganisms can produce a variety of antibiotics such as chaetocin and chaetoglobosin, and are widely studied as biocontrol inoculants for plant pathogens. At present, there is no report about the use of isolated C. globosum strain in controlling FCR of wheat.
  • the present disclosure provides use of a C. globosum strain in controlling FCR of wheat. Field experiments conclude that after coating of wheat seeds with Chaetomium. globosum strain 12XP1-2-3, diseased stem rate, disease rating, and disease index are reduced by 19-41.3%, 43.7%, and 4.7-45.4%, respectively.
  • the present disclosure provides use of a C. globosum strain in controlling FCR of wheat;
  • the present disclosure further provides use of a biocontrol agent containing the above C. globosum strain in controlling FCR of wheat.
  • the biocontrol agent may be prepared by: multiplying the C. globosum strain to obtain a spore suspension, and dispersing the spore suspension in a sodium carboxymethyl cellulose solution to obtain the biocontrol agent.
  • the sodium carboxymethyl cellulose solution may have a mass fraction of 4%, and the spore suspension and the sodium carboxymethyl cellulose solution may have a volume ratio of 3:1.
  • wheat seeds are coated at a coating concentration of 5.0-5.3 ⁇ 10 4 ascospores per seed.
  • Biomaterial Chaetomium globosum 12XP1-2-3; Taxonomic designation: Chaetomium globosum , deposited at the China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology Chinese Academy of Sciences, NO. 3, Yard 1, West Beichen Road, Chaoyang District, Beijing, with accession number of CGMCC No. 17183.
  • CGMCC General Microbiological Culture Collection Center
  • the C. globosum strain 12XP1-2-3 provided by the present disclosure may be used to control FCR of wheat. Field experiments conclude that after seed dressing with the C. globosum strain 12XP1-2-3, the diseased stem rate, disease rating, and disease index of wheat may be reduced by 19-41.3%, 43.7%, and 4.7-45.4%, respectively.
  • FIG. 1 shows the plate antagonistic effect of C. globosum strain 12XP1-2-3 on Fusarium pseudograminearum G14LY24-2.
  • globosum strain 12XP1-2-3 was picked on a PDA medium, and cultured at 20° C. for 5-7 days; five marginal hyphal pellets were picked and placed evenly on a PDA plate covered with sterilized cellophane. After 3-5 days, hyphae were scraped with a small sterilized iron shovel, quick-frozen with liquid nitrogen, and stored in a refrigerator at ⁇ 20° C.
  • primers ITS1 (5′-TCC GTA GGT GAA CCT GCG G-3′) and ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′) were used to amplify DNA fragments containing ITS1, 5.8S rDNA and ITS2 in the test strain.
  • Amplification conditions were as follows: PCR program was: 95° C. for 3 mM; 35 cycles of 95° C. for 45 s, 50° C. for 30 s, and 72° C. for 1 min; 72° C. for 10 mM.
  • BLAST sequence alignment analysis was performed in NCBI. The sequence alignment result concluded that the biocontrol inoculant 12XP1-2-3 was C. globosum .
  • the C. globosum strain 12XP1-2-3 was picked with a sterile toothpick and activated on a PDA; the culture condition was 25° C. on 12 h light/12 h dark cycle.
  • the wheat crown rot fungus G14LY24-2 was activated on a PDA; the culture condition was 25° C. without light.
  • a puncher with an inner diameter of 0.5c m was used to beat the edges of the colonies of C. globosum and wheat crown rot fungus into a plurality of mycelium discs. Two sections were marked 2.5 cm away from the center of the PDA Petri dish; mycelium discs of C.
  • Wheat grains are soaked for 12 h, sterilized for 60 min, allowed to stand for two days, sterilized for 30-60 min, and cooled for later use.
  • Fusarium pseudograminearum G14LY24-2 was selected on a PDA plate. After growing for three days, marginal hyphae were picked to grow on a fresh PDA plate. After three days, the hyphal pellets were plated into a wheat grain polyethylene bag with sterilized wheat grains. The hyphal pellets grew at room temperature for two weeks until the fungi grew all over wheat grains, and stored in a refrigerator at 4° C. after air-drying.
  • the wheat cultivar in 2018 was “Bainong 207”
  • the wheat cultivar in 2019 was “Aikang 58”
  • the control agent was 30 g/L difenoconazole
  • the seeds were dressed in an agent-to-seed ratio of 1:333.
  • Field sampling method At the seedling stage, the emergence rate was investigated, and the plant height, tap root length, fresh weight, dry weight, and colonization ability of C. globosum were measured; at the tillering stage, the morbidity was investigated, and the plant height, tap root length, fresh weight, dry weight, tiller number, and colonization ability of C. globosum were measured; the morbidity was investigated at the jointing stage; the white ear rate was investigated at the filling stage. The method was to investigate the number of white heads and total number of ears per 1 m2 plot and calculate the white ear rate of the plot. The diseased stem rate was investigated at the maturing stage; 20 stems were sampled at five points in each plot, a total of 100 stems for each treatment were brought back to the laboratory, and leaf sheaths were removed thoroughly to determine the disease rating.
  • the yield was determined during harvest. The results are shown in Table 2.
  • the wheat cultivar in 2018 is “Bainong 207”, in the control treatment, the diseased stem rate is 57.8%, and the average disease rating is 0.7; for the seed coating with C. globosum strain 12XP1-2-3, the diseased stem rate and the average disease rating are 33.9% and 0.7, respectively;
  • the wheat cultivar in 2019 is “Aikang 58”, in the control treatment, the diseased stem rate is 50.4%, and the average disease rating is 1.6; for the seed coating with C. globosum strain 12XP1-2-3, the diseased stem rate and the average disease rating are 40.8% and 1.6, respectively.
  • coating of seeds with the C. globosum strain 12XP1-2-3 provided by the present disclosure can not only reduce the white head rate, but also lower the disease rating and disease index.

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US17/233,732 2020-08-18 2021-04-19 Use of Chaetomium globosum strain in controlling fusarium crown rot of wheat Active 2041-08-22 US11998016B2 (en)

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CN202010832744.5A CN111996125B (zh) 2020-08-18 2020-08-18 一种球毛壳菌在防治小麦茎基腐病中的应用
CN202010832744.5 2020-08-18

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CN112080433A (zh) * 2020-08-19 2020-12-15 河南省农业科学院植物保护研究所 一种球毛壳菌及其在小麦促生增产中的应用
CN117512180B (zh) * 2023-12-01 2024-06-07 中国农业大学 小麦茎基腐抗病位点Qfcr.cau.2A的KASP分子标记及其应用

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US5041290A (en) * 1985-10-02 1991-08-20 Ciba-Geigy Corporation Method of protecting useful plants from diseases caused by soil-borne and seed-borne pathogens by treating seeds with cultures of microorganisms

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CN102311925B (zh) * 2011-08-03 2014-05-07 山东农业大学 一种植物内生真菌球毛壳菌株、微生物菌剂及其应用
CN105229141A (zh) * 2013-03-15 2016-01-06 拉勒曼德匈牙利流动管理有限责任公司 用于木质纤维素和相关低聚物的水解的β-葡糖苷酶的表达
CN104877919B (zh) * 2015-06-08 2017-12-29 鲁东大学 一种球毛壳菌及其应用
CN108676745B (zh) * 2018-05-22 2021-04-13 淮阴工学院 一株防治小麦茎基腐病和赤霉病的解木糖赖氨酸芽孢杆菌lx024及其应用
CN109593658B (zh) * 2018-12-27 2020-11-06 河南科技大学 一种具有抗生作用的内生真菌zxl-szy-r-9及其应用

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US5041290A (en) * 1985-10-02 1991-08-20 Ciba-Geigy Corporation Method of protecting useful plants from diseases caused by soil-borne and seed-borne pathogens by treating seeds with cultures of microorganisms

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M.R. Fernandez et al., Root and Crown Rot of Wheat ,Prairie Soils & Crops Journal, vol. 4⋅ 2011, pp. 151-157. *

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