Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
  • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
  • A61K31/404—Indoles, e.g. pindolol
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
  • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
  • A61P31/14—Antivirals for RNA viruses
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
  • C07D209/12—Radicals substituted by oxygen atoms
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
  • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/30—Indoles; Hydrogenated indoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to carbon atoms of the hetero ring
  • C07D209/42—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
  • G01N30/02—Column chromatography
  • G01N30/62—Detectors specially adapted therefor
  • G01N30/72—Mass spectrometers
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
  • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
  • C07B2200/13—Crystalline forms, e.g. polymorphs
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
  • G01N30/02—Column chromatography
  • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
  • G01N2030/027—Liquid chromatography
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
  • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
  • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

• the present invention relates to mono- or di-substituted indole compounds, methods to prevent or treat dengue viral infections by using said compounds and also relates to said compounds for use as a medicine, more preferably for use as a medicine to treat or prevent dengue viral infections.
• the present invention furthermore relates to pharmaceutical compositions or combination preparations of the compounds, to the compositions or preparations for use as a medicine, more preferably for the prevention or treatment of dengue viral infections.
• the invention also relates to processes for preparation of the compounds.
• Flaviviruses which are transmitted by mosquitoes or ticks, cause life-threatening infections in man, such as encephalitis and hemorrhagic fever.
• Four distinct, but closely related serotypes of the flavivirus dengue are known, so-called DENV1, -2, -3, and -4.
• Dengue is endemic in most tropical and sub-tropical regions around the world, predominantly in urban and semi-urban areas. According to the World Health Organization (WHO), 2.5 billion people of which 1 billion children are at risk of DENV infection (WHO, 2002).
• WHO World Health Organization
• dengue hemorrhagic fever and dengue shock syndrome [DSS]
• DHF dengue hemorrhagic fever
• DSS dengue shock syndrome
• AD antibody-dependent enhancement
• DHF dengue hemorrhagic fever
• the mosquitoes that carry dengue including Aedes aegypti and Aedes albopictus (tiger mosquito), are moving north on the globe. According to the United States (US) Centers for Disease Control and Prevention (CDC), both mosquitoes are currently omnipresent in southern Texas. The spread north of dengue-carrying mosquitoes is not confined to the US, but has also been observed in Europe.
• US United States
• CDC Centers for Disease Control and Prevention
• the present invention now provides compounds, mono- or di-substituted indole derivatives, which show high potent activity against all four (4) serotypes of the Dengue virus. Also the compounds according to the invention possess a good pharmacokinetic profile and surprisingly these specific compounds show an improved chiral stability.
• the present invention is based on the unexpected finding that at least one of the above-mentioned problems can be solved by the current compounds of the invention.
• the present invention provides compounds which have been shown to possess potent antiviral activity against all four (4) serotypes currently known.
• the present invention furthermore demonstrates that these compounds efficiently inhibit proliferation of Dengue virus (DENV). Therefore, these compounds constitute a useful class of potent compounds that can be used in the treatment and/or prevention of viral infections in animals, mammals and humans, more specifically for the treatment and/or prevention of infections with Dengue viruses.
• DEV Dengue virus
• the present invention furthermore relates to the use of such compounds as medicines and to their use for the manufacture of medicaments for treating and/or preventing viral infections, in particular with viruses belonging to the family of the Dengue viruses in animals or mammals, more in particular in humans.
• the invention also relates to methods for the preparation of all such compounds and to pharmaceutical compositions comprising them in an effective amount.
• the present invention also relates to a method of treatment or prevention of dengue viral infections in humans by the administration an effective amount of one or more such compounds, or a pharmaceutically acceptable salt thereof optionally in combination with one or more other medicines, like another antiviral agent, to a patient in need thereof.
• R 1 is H, R 2 is F and R 3 is H, F or CH 3 ;
• R 1 is F or CH 3 , R 2 is OCH 3 and R 3 is H;
• R 1 is H, R 2 is Cl and R 3 is H or CH 3 ;
• R 1 is F, R 2 is H and R 3 is CH 3 ;
• R 1 is H, R 2 is OCH 3 and R 3 is Cl,
• R 1 is F, R 2 is F and R 3 is H, R 1 is H, R 2 is OCH 3 and R 3 is Cl,
• R 1 is F, R 2 is F and R 3 is H,
• R 1 is H, R 2 is OCH 3 and R 3 is CH 3 or R 1 is CH 3 ,
• R 2 is H and R 3 is F.
• Part of the current invention is also a pharmaceutical composition
• a pharmaceutical composition comprising a compound of formula (I) or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof together with one or more pharmaceutically acceptable excipients, diluents or carriers.
• Pharmaceutically acceptable salts of the compounds of formula (I) include the acid addition and base salts thereof. Suitable acid addition salts are formed from acids which form non-toxic salts. Suitable base salts are formed from bases which form non-toxic salts.
• the compounds of the invention may also exist in un-solvated and solvated forms.
• solvate is used herein to describe a molecular complex comprising the compound of the invention and one or more pharmaceutically acceptable solvent molecules, for example, ethanol.
• polymorph refers to the ability of the compound of the invention to exist in more than one form or crystal structure.
• the compounds of the present invention may be administered as crystalline or amorphous products. They may be obtained for example as solid plugs, powders, or films by methods such as precipitation, crystallization, freeze drying, spray drying, or evaporative drying. They may be administered alone or in combination with one or more other compounds of the invention or in combination with one or more other drugs. Generally, they will be administered as a formulation in association with one or more pharmaceutically acceptable excipients.
• excipient is used herein to describe any ingredient other than the compound(s) of the invention. The choice of excipient depends largely on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature of the dosage form.
• compositions of the present invention may be formulated into various pharmaceutical forms for administration purposes.
• compositions there may be cited all compositions usually employed for systemically administering drugs.
• an effective amount of the particular compound, optionally in addition salt form, as the active ingredient is combined in intimate admixture with a pharmaceutically acceptable carrier, which carrier may take a wide variety of forms depending on the form of preparation desired for administration.
• a pharmaceutically acceptable carrier which carrier may take a wide variety of forms depending on the form of preparation desired for administration.
• These pharmaceutical compositions are desirably in unitary dosage form suitable, for example, for oral or rectal administration.
• any of the usual pharmaceutical media may be employed such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs, emulsions, and solutions; or solid carriers such as starches, sugars, kaolin, diluents, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules, and tablets. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit forms, in which case solid pharmaceutical carriers are obviously employed. Also included are solid form preparations that can be converted, shortly before use, to liquid forms.
• Unit dosage form refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
• unit dosage forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, suppositories, injectable solutions or suspensions and the like, and segregated multiples thereof.
• an effective daily amount would be from 0.01 mg/kg to 50 mg/kg body weight, more preferably from 0.1 mg/kg to 10 mg/kg body weight. It may be appropriate to administer the required dose as two, three, four or more sub-doses at appropriate intervals throughout the day. Said sub-doses may be formulated as unit dosage forms, for example, containing 1 to 1000 mg, and in particular 5 to 200 mg of active ingredient per unit dosage form.
• the exact dosage and frequency of administration depends on the particular compound of formula (I) used, the particular condition being treated, the severity of the condition being treated, the age, weight and general physical condition of the particular patient as well as other medication the individual may be taking, as is well known to those skilled in the art. Furthermore, it is evident that the effective amount may be lowered or increased depending on the response of the treated subject and/or depending on the evaluation of the physician prescribing the compounds of the instant invention. The effective amount ranges mentioned above are therefore only guidelines and are not intended to limit the scope or use of the invention to any extent.
• isotopes of hydrogen include tritium and deuterium and isotopes of carbon include C-13 and C-14.
• the present compounds used in the current invention may also exist in their stereo-chemically isomeric form, defining all possible compounds made up of the same atoms bonded by the same sequence of bonds but having different three-dimensional structures, which are not interchangeable. Unless otherwise mentioned or indicated, the chemical designation of compounds encompasses the mixture of all possible stereo-chemically isomeric forms, which said compounds might possess.
• Said mixture may contain all diastereomers and/or enantiomers of the basic molecular structure of said compound.
• All stereo-chemically isomeric forms of the compounds used in the present invention either in pure form or in admixture with each other are intended to be embraced within the scope of the present invention including any racemic mixtures or racemates.
• stereoisomerically pure concerns compounds or intermediates having a stereoisomeric excess of at least 80% (i. e. minimum 90% of one isomer and maximum 10% of the other possible isomers) up to a stereoisomeric excess of 100% (i.e.
• Pure stereoisomeric forms of compounds and intermediates used in this invention may be obtained by the application of art-known procedures.
• enantiomers may be separated from each other by the selective crystallization of their diastereomeric salts with optically active acids or bases. Examples thereof are tartaric acid, dibenzoyltartaric acid, ditoluoyltartaric acid and camphosulfonic acid.
• enantiomers may be separated by chromatographic techniques using chiral stationary phases.
• Said pure stereochemically isomeric forms may also be derived from the corresponding pure stereochemically isomeric forms of the appropriate starting materials, provided that the reaction occurs stereospecifically.
• a specific stereoisomer is desired, said compound will be synthesized by stereospecific methods of preparation. These methods will advantageously employ enantiomerically pure starting materials.
• the introduction of an aniline moiety in alpha position to the carbonyl moiety of the compounds of general formula V can be accomplished by a reaction sequence that involves for example bromination of V with a reagent like for example phenyltrimethylammonium tribromide in a suitable solvent like for example THF, to provide the compounds of general formula VI, and subsequent reaction of the compounds of general formula VI with 2-(3-amino-5-methoxyphenoxy)ethanol (VII) in a suitable solvent like for example CH 3 CN, and typically using a base like for example TEA or DIPEA, to provide the compounds of general formula I as racemic mixtures.
• a reaction sequence that involves for example bromination of V with a reagent like for example phenyltrimethylammonium tribromide in a suitable solvent like for example THF, to provide the compounds of general formula VI, and subsequent reaction of the compounds of general formula VI with 2-(3-amino-5-methoxyphenoxy)ethanol (VII) in a suitable solvent like for
• Chiral separation of the compounds of general formula I can be performed by for example chiral chromatography to provide the Enantiomers A and B of general formula I.
• HPLC High Performance Liquid Chromatography
• MS Mass Spectrometer
• SQL Single Quadrupole Detector
• MSD Mass Selective Detector
• RT room temperature
• BEH bridged ethylsiloxane/silica hybrid
• DAD Diode Array Detector
• HSS High Strength silica
• the SFC measurement was performed using an Analytical Supercritical fluid chromatography (SFC) system composed by a binary pump for delivering carbon dioxide (CO 2 ) and modifier, an autosampler, a column oven, a diode array detector equipped with a high-pressure flow cell standing up to 400 bars. If configured with a Mass Spectrometer (MS) the flow from the column was brought to the (MS). It is within the knowledge of the skilled person to set the tune parameters (e.g. scanning range, dwell time . . . ) in order to obtain ions allowing the identification of the compound's nominal monoisotopic molecular weight (MW). Data acquisition was performed with appropriate software.
• SFC Analytical Supercritical fluid chromatography
• Values are either peak values or melt ranges, and are obtained with experimental uncertainties that are commonly associated with this analytical method.
• DSC823e (Indicated as DSC)
• melting points were determined with a DSC823e (Mettler-Toledo). Melting points were measured with a temperature gradient of 10° C./minute. Maximum temperature was 300° C.
• [ ⁇ ] ⁇ T (100 ⁇ )/(l ⁇ c): where l is the path length in dm and c is the concentration in g/100 ml for a sample at a temperature T (° C.) and a wavelength ⁇ (in nm). If the wavelength of light used is 589 nm (the sodium D line), then the symbol D might be used instead.
• the sign of the rotation (+ or ⁇ ) should always be given. When using this equation the concentration and solvent are always provided in parentheses after the rotation. The rotation is reported using degrees and no units of concentration are given (it is assumed to be g/100 ml).
• Example 1 Synthesis of 1-(6-fluoro-1H-indol-3-yl)-2-(4-fluoro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 1) and Chiral Separation into Enantiomers 1A and 1B
• 2-(4-Fluoro-2-methoxyphenyl)acetic acid [CAS 886498-61-9] (28.9 g, 157 mmol) was added in small portions to thionyl chloride (150 mL) and the resulting solution was stirred overnight at room temperature. The solvent was concentrated under reduced pressure and co-evaporated with toluene to give 2-(4-fluoro-2-methoxyphenyl)acetyl chloride 1a (31.8 g) as an oily residue that was used without further purification in the next step.
• Enantiomer 1A (2.4 g) as an amorphous white powder.
• Enantiomer 1B (4.6 g) was solidified by trituration of the oily residue with a mixture of MeOH (10 mL) and water (15 mL) under vigorous stirring for 25 min. The solids were filtered off and subsequently crystallized from a mixture of MeOH (70 mL) and water (30 mL) under stirring at room temperature for 3 h. A white precipitate was filtered off and dried under vacuum at 50° C. to provide Enantiomer 1B (2.50 g) as an amorphous white powder.
• Example 1.1 Chiral Stability of Enantiomer 1A at pH 7.4
• the chiral stability of Enantiomer 1A was evaluated by determination of the enantiomeric excess (ee %) after incubation for 24 h and 48 h in a buffered solution at pH 7.4 at 40° C. and 60° C. To assess the influence of the methoxy-substituent of Enantiomer 1A (R ⁇ OMe) on the stability against racemization, the chiral stability of Enantiomer 1′A (R ⁇ H) was tested under the same conditions.
• Example 2 synthesis 2-(4-fluoro-2-methoxyphenyl)-1-(6-fluoro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 2) and Chiral Separation into Enantiomers 2A and 2B
• the reaction mixture was diluted by the addition of 2-methyl-THF (150 mL) and stirred for 30 min at room temperature.
• Na 2 SO 4 (30 g) was added and after stirring for 30 min, the mixture was filtered over Dicalite®.
• the filter cake was washed several times with 2-methyl-THF and the combined filtrates were concentrated in vacuum to a residual volume of 25 mL. After standing for 2 h, a precipitate was filtered off and dried under vacuum to provide 1-(6-chloro-1H-indol-3-yl)-2-(4-fluoro-2-methoxyphenyl)ethanone 4a (2.85 g).
• the residue was purified by flash chromatography on silica (Stationary phase: Biotage® SnapUltra Silica 50 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 60/30/10).
• the desired fractions were combined and evaporated and subsequently purified via Preparative HPLC (Stationary phase: Uptisphere® C18 ODB-10 ⁇ m, 200 g, 5 cm, Mobile phase: 0.25% NH 4 HCO 3 solution in water, CH 3 CN).
• the product fractions were combined and evaporated.
• the second eluted product was further purified by flash chromatography on silica (stationary phase: Biotage® SnapUltra GraceReveleris® Silica 12 g, Mobile phase: heptane/EtOAc gradient 100/0 to 0/100).
• the product fractions were combined and evaporated under reduced pressure.
• the residue was solidified by lyophilization from CH 3 CN/water to provide Enantiomer 4B (160 mg) as an amorphous powder.
• Example 5 Synthesis of 1-(6,7-difluoro-1H-indol-3-yl)-2-(4-fluoro-2-methoxy-phenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 5) and Chiral Separation into Enantiomers 5A and 5B
• Enantiomer 5A (414 mg) as an amorphous white powder.
• the second eluted product was further purified by column chromatography on silica (Stationary phase: Silica 25 g HP-Spher 25 ⁇ m, Mobile phase: CH 2 Cl 2 /MeOH gradient 100/0 to 0/100).
• the product fractions were concentrated to a residual amount of 2 mL.
• a white precipitate was filtered off, washed several times with CH 2 Cl 2 and dried under vacuum at 50° C. to provide Enantiomer 5B (437 mg) as an amorphous white powder.
• phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.8 g, 10.1 mmol) in THF (90 mL) was added dropwise to a mixture of 2-(4-fluoro-2-methoxyphenyl)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone 6a (3.15 g, 9.6 mmol) in THF (90 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure.
• Fraction 1 was purified again by flash chromatography on silica gel (15-40 ⁇ m, 12 g, CH 2 Cl 2 /CH 3 OH 99.5/0.5). The pure fractions were collected and evaporated to dryness to give 207 mg. The compound was solidified from Et 2 O/heptane and a few drops of CH 3 CN to afford Enantiomer 6A (173 mg) as an amorphous powder.
• Fraction 2 was purified again by flash chromatography on silica gel (15-40 ⁇ m, 12 g, CH 2 Cl 2 /CH 3 OH 99.5/0.5). The pure fractions were collected and evaporated to dryness to afford 204 mg. The compound was solidified from Et 2 O/heptane and a few drops of CH 3 CN to afford Enantiomer 6B (158 mg) as an amorphous powder.
• Example 7 Synthesis of 2-(4-fluoro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 7) and Chiral Separation into Enantiomers 7A and 7B
• phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.22 g, 8.56 mmol) in THF (80 mL) was added dropwise to a mixture of 2-(4-fluoro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)ethanone 7a (2.7 g, 8.15 mmol) in THF (80 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h.
• the organic layer was separated, dried over MgSO 4 , filtered, and the solvent was evaporated under reduced pressure.
• the crude compound was purified by column chromatography on silica gel (15-40 ⁇ m, 40 g in CH 2 Cl 2 /MeOH/NH 4 OH (99/1/0.1)). The fractions containing Compound 7 were combined and the solvent was evaporated under reduced pressure. The residue was dissolved in CH 2 Cl 2 and washed with 1N HCl. The organic layer was separated, dried over MgSO 4 , filtered and the solvent was concentrated under reduced pressure.
• the Enantiomers were separated via Preparative Chiral SFC (Stationary phase: Chiralpak® IC 5 ⁇ m 250 ⁇ 30 mm, Mobile phase: 60% CO 2 , 40% MeOH) to give 70 mg of Compound 2 as a racemic mixture, 104 mg of the first eluted enantiomer and 100 mg of the second eluted enantiomer.
• the first eluted enantiomer was crystallized from CH 3 CN/Et 2 O to afford Enantiomer 7A (76 mg).
• the second eluted enantiomer was crystallized from CH 3 CN/Et 2 O to afford Enantiomer 7B (62 mg).
• Example 8 Synthesis of 1-(7-chloro-6-methoxy-1H-indol-3-yl)-2-(4-fluoro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 8) and Chiral Separation into Enantiomers 8A and 8B
• phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.06 g, 8.15 mmol) in THF (80 mL) was added dropwise to a mixture of 1-(7-chloro-6-methoxy-1H-indol-3-yl)-2-(4-fluoro-2-methoxyphenyl)ethanone 8a (2.7 g, 7.76 mmol) in THF (80 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h. The precipitate was filtered off, washed with EtOAc.
• the enantiomers were separated via Chiral SFC (Stationary phase: Chiralpak® IC 5 ⁇ m 250 ⁇ 30 mm, Mobile phase: 55% CO 2 , 45% MeOH) to give 184 mg of the first eluted enantiomer and 190 mg of the second eluted enantiomer.
• the first eluted enantiomer was crystallized from heptane/diisopropylether/CH 3 CN to afford Enantiomer 8A (135 mg) as an amorphous solid.
• the second eluted enantiomer was crystallized from heptane/diisopropylether/CH 3 CN to afford Enantiomer 8B (150 mg) as an amorphous solid.
• Example 10 Synthesis of 1-(5,6-difluoro-1H-indol-3-yl)-2-(4-fluoro-2-methoxy-phenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 10) and Chiral Separation into Enantiomers 10A and 10B
• a second purification was performed via Reverse Phase HPLC (Stationary phase: X-Bridge®-C18 5 ⁇ m 19 ⁇ 100 mm, Mobile phase: gradient from 80% formic acid 0.1%/20% CH 3 CN to 10% formic acid 0.1%/90% CH 3 CN) to give 1-(5,6-difluoro-1H-indol-3-yl)-2-(4-fluoro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxy-phenyl)amino)ethanone (Compound 10, 499 mg) as a racemic mixture.
• the enantiomers of Compound 10 (459 mg) were separated via Preparative Chiral SFC (Stationary phase: Chiralcel® OD-H 5 ⁇ m 250 ⁇ 30 mm, Mobile phase: 60% CO 2 , 40% MeOH) to give 208 mg of the first eluted enantiomer and 221 mg of the second eluted enantiomer.
• the first eluted enantiomer was solidified from heptane to afford Enantiomer 10A (168 mg).
• the second eluted enantiomer was solidified from heptane to afford Enantiomer 10B (174 mg).
• Example 11 Synthesis of 2-(4-fluoro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)-1-(6-methoxy-7-methyl-1H-indol-3-yl)ethanone (Compound 11) and Chiral Separation into Enantiomers 11A and 11B
• Enantiomer 11A 255 mg
• Enantiomer 11B was further purified by column chromatography on silica gel (Stationary phase: SNAP Ultra 10 g, Mobile Phase CH 2 Cl 2 /MeOH gradient 100/0 to 98/2). The product fractions were combined, the solvent was evaporated under reduced pressure and the residue was dried under vacuum at 50° C. to provide Enantiomer 11B (270 mg) as an amorphous white powder.
• Example 12 Synthesis of 2-(4-fluoro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 12) and Chiral Separation into Enantiomers 12A and 12B
• the antiviral activity of all the compounds of the invention was tested against DENV-2 16681 strain which was labeled with enhanced green fluorescent protein (eGPF).
• the culture medium consists of minimal essential medium supplemented with 2% of heat-inactivated fetal calf serum, 0.04% gentamycin (50 mg/mL) and 2 mM of L-glutamine.
• Vero cells, obtained from ECACC, were suspended in culture medium and 25 ⁇ L was added to 384-well plates (2500 cells/well), which already contain the antiviral compounds. Typically, these plates contain a 4-fold serial dilution of 9 dilution steps of the test compound at 200 times the final concentration in 100% DMSO (200 nL).
• each compound concentration is tested in quadruplicate (final concentration range: 25 ⁇ M-0.00038 ⁇ M).
• each plate contains wells which are assigned as virus controls (containing cells and virus in the absence of compound), cell controls (containing cells in the absence of virus and compound) and medium controls (containing medium in the absence of cells, virus and compounds).
• virus controls containing cells and virus in the absence of compound
• cell controls containing cells in the absence of virus and compound
• medium controls containing medium in the absence of cells, virus and compounds.
• 25 ⁇ L of culture medium was added instead of Vero cells. Once the cells were added to the plates, the plates were incubated for 30 minutes at room temperature to allow the cells to distribute evenly within the wells. Next, the plates were incubated in a fully humidified incubator (37° C., 5% CO 2 ) until the next day.
• DENV-2 strain 16681 labeled with eGFP, was added at a multiplicity of infection (MOI) of 0.5. Therefore, 15 ⁇ L of virus suspension was added to all the wells containing test compound and to the wells assigned as virus control. In parallel, 15 ⁇ L of culture medium was added to the medium and cell controls. Next, the plates were incubated for 3 days in a fully humidified incubator (37° C., 5% CO 2 ). At the day of the read out, the eGFP fluorescence was measured using an automated fluorescence microscope at 488 nm (blue laser). Using an in-house LIMS system, inhibition dose response curves for each compound were calculated and the half maximal effective concentration (EC 50 ) was determined.
• MOI multiplicity of infection
• the EC 50 represents the concentration of a compound at which the virus replication is inhibited with 50%, as measured by a 50% reduction of the eGFP fluorescent intensity compared to the virus control.
• the EC 50 is calculated using linear interpolation (Table 1).
• resazurin a cell viability stain
• the resazurin assay is based on the reduction of the blue resazurin by NADH, produced by the cells, into the highly fluorescent product, resorufin.
• the formation of pink fluorescent resorufin is directly related to the number of viable cells in the well.
• the plates were incubated for an additional 5 hours in a fully humidified incubator (37° C., 5% CO 2 ).
• the antiviral activity of the compounds of the invention was tested against DENV-1 strain TC974#666 (NCPV), DENV-2 strain 16681, DENV-3 strain H87 (NCPV) and DENV-4 strains H241 (NCPV) and EDEN (SG/06K2270DK1/2005; GenBank accession number QG398256) in a RT-qPCR assay. Therefore, Vero cells were infected with either DENV-1, or -2, or -3, or -4 in the presence or absence of test compounds.
• the cells were lysed and cell lysates were used to prepare cDNA of both a viral target (the 3′UTR of DENV; table 2) and a cellular reference gene ( ⁇ -actin, table 2). Subsequently, a duplex real time PCR was performed on a Lightcycler480 instrument. The generated Cp value is inversely proportional to the amount of RNA expression of these targets. Inhibition of DENV replication by a test compound result in a shift of Cp's for the 3′UTR gene. On the other hand, if a test compound is toxic to the cells, a similar effect on ⁇ -actin expression will be observed.
• the comparative ⁇ Cp method is used to calculate EC 50 , which is based on the relative gene expression of the target gene (3′UTR) normalized with the cellular housekeeping gene ( ⁇ -actin). In addition, CC 50 values were determined based on the Cp values acquired for the housekeeping gene ⁇ -actin.
• FAM, HEX HEX
• BHQ1 elements are indicated in bold and italics.
• nucleotide sequence of the primers and probes were selected from the conserved region in the 3′UTR region of the dengue virus genome, based on the alignment of 300 nucleotide sequences of the four dengue serotypes deposited in Genbank (Gong et al., 2013, Methods Mol Biol, Chapter 16).
• the culture medium consisted of minimal essential medium supplemented with 2% of heat-inactivated fetal calf serum, 0.04% gentamycin (50 mg/mL) and 2 mM of L-glutamine.
• Vero cells obtained from ECACC, were suspended in culture medium and 75 ⁇ L/well was added in 96-well plates (10000 cells/well), which already contain the antiviral compounds. Typically, these plates contain a 4-fold serial dilution of 9 dilution steps of the test compound at 200 times the final concentration in 100% DMSO (500 nL; final concentration range: 25 ⁇ M-0.00038 ⁇ M).
• each plate contains wells which are assigned as virus controls (containing cells and virus in the absence of compound) and cell controls (containing cells in the absence of virus and compound).
• virus controls containing cells and virus in the absence of compound
• cell controls containing cells in the absence of virus and compound.
• mix A (table 3A) was prepared and 7.57 ⁇ L/well was dispensed in a 96-well plate.
• a RT-qPCR mix was prepared, mix C (table 4A), dispensed in 96-well LightCycler qPCR plates to which 3 ⁇ L of cDNA was added and the qPCR was performed according to the conditions in table 4B on a LightCycler 480.
• dose response curves for each compound were calculated and the half maximal effective concentration (EC 50 ) and the half maximal cytotoxic concentration (CO 50 ) were determined (Tables 6-9).
• Vero cells (4 ⁇ 10 4 ) were seeded in 96-well plates. After one day, cell culture medium was replaced with 100 ⁇ L assay medium containing a 2 ⁇ , 3 ⁇ or 5 ⁇ serial dilution of the compound (concentration range: 50 ⁇ g/mL-0.00038 ⁇ g/mL, 50 ⁇ g/mL-0.0076 ⁇ g/mL, and 50 ⁇ g/mL-0.00013 ⁇ g/mL, respectively) and 100 ⁇ L of dengue virus dilution, where a Ct of ⁇ 20 was achieved in RTqPCR.
• the cell monolayer was washed 3 times with assay medium to remove residual, non-adsorbed virus and cultures were further incubated for either 4 days (DENV-2 NGC-Tongalike) or 7 days (DENV-1 Djibouti strain D1/H/IMTSSA/98/606, DENV-3 strain H87 prototype, DENV-4 strain H241′) in the presence of the inhibitor.
• Supernatant was harvested and viral RNA load was determined by real-time quantitative RT-PCR.
• the 50% effective concentration (EC 50 ) which is defined as the compound concentration that is required to inhibit viral RNA replication by 50%, was determined using logarithmic interpolation (Tables 7 and 8).
• RNA was isolated from 100 ⁇ L supernatant with the NucleoSpin 96 Virus kit (Filter Service, Duren, Germany) as described by the manufacturer.
• the sequences of the TaqMan primers (DENV-For, DENV-Rev; Table 5) and TaqMan probes (DENV-Probe Table 5) were selected from non-structural gene 3 (NS3) or NS5, of the respective flaviviruses using Primer Express software (version 2.0; Applied Biosystems, Lennik, Belgium).
• the TaqMan probe was fluorescently labelled with 6-carboxyfluorescein (FAM) at the 5′ end as the reporter dye, and with minor groove binder (MGB) at the 3′ end as the quencher (Table 5).
• RT-PCR was performed in a total volume of 25 ⁇ L, containing 13.9375 ⁇ L H 2 O, 6.25 ⁇ L master mix (Eurogentec, Seraing, Belgium), 0.375 ⁇ L forward primer, 0.375 ⁇ L reverse primer, 1 ⁇ L probe, 0.0625 ⁇ L reverse transcriptase (Eurogentec) and 3 ⁇ L sample.
• RT-PCR was performed using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Branchburg, N.J., USA) using the following conditions: 30 min at 48° C. and 10 min at 95° C., followed by 40 cycles of 15 s at 95° C. and 1 min at 60° C. The data was analyzed using the ABI PRISM 7500 SDS software (version 1.3.1; Applied Biosystems). For absolute quantification, standard curves were generated using 10-fold dilutions of template preparations of known concentrations.
• the 50% cytotoxic concentration i.e., the concentration that reduces the total cell number with 50%; CC 50 ) was calculated using linear interpolation (Tables 7 and 8).

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