UA89398U - Immunoperoxidase test for virologic diagnostics of porcine circovirus infection - Google Patents
Immunoperoxidase test for virologic diagnostics of porcine circovirus infectionInfo
- Publication number
- UA89398U UA89398U UAU201309587U UAU201309587U UA89398U UA 89398 U UA89398 U UA 89398U UA U201309587 U UAU201309587 U UA U201309587U UA U201309587 U UAU201309587 U UA U201309587U UA 89398 U UA89398 U UA 89398U
- Authority
- UA
- Ukraine
- Prior art keywords
- cells
- microplate
- cell
- virologic
- diagnostics
- Prior art date
Links
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
An immunoperoxidase test for virologic diagnostics of porcine circovirus infection involves inoculation of sensitive culture cells onto 96-cell plastic microplate with flat bottom, dilution of test virus-containg material by ten-fold dilution technique in 96-cell plastic microplates with round bottom followed by transferring thereof into microplate cells with monolayer of cell culture, incubation in COconditions of temperature-regulated chamber for 7 days, removal of culture medium from microplate cells with washing monolayer of cell culture, addition of fixing buffer into all microplate cells, stepwise addition of working dilution of specific blood serum, working dilution of conjugate, substrate solution into all microplate cells, termination of reaction by addition of stop solution into each microplate cell and reaction recording by means of inverted light microscope wherein infected cells have the appearance of isolated cells or cell clumps with deep red colored cytoplasma and pale nucleus. The method is used for virologic diagnostics of porcine circovirus infection in domestic and wild pigs by investigation of biological material (blood, suspension of organs, biological fluids) or by determination of infectious activity titer of vaccine, reference strains or field isolates of II type circovirus.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UAU201309587U UA89398U (en) | 2013-07-31 | 2013-07-31 | Immunoperoxidase test for virologic diagnostics of porcine circovirus infection |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UAU201309587U UA89398U (en) | 2013-07-31 | 2013-07-31 | Immunoperoxidase test for virologic diagnostics of porcine circovirus infection |
Publications (1)
Publication Number | Publication Date |
---|---|
UA89398U true UA89398U (en) | 2014-04-25 |
Family
ID=52281837
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UAU201309587U UA89398U (en) | 2013-07-31 | 2013-07-31 | Immunoperoxidase test for virologic diagnostics of porcine circovirus infection |
Country Status (1)
Country | Link |
---|---|
UA (1) | UA89398U (en) |
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2013
- 2013-07-31 UA UAU201309587U patent/UA89398U/en unknown
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