UA89399U - Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome - Google Patents

Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome

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Publication number
UA89399U
UA89399U UAU201309588U UAU201309588U UA89399U UA 89399 U UA89399 U UA 89399U UA U201309588 U UAU201309588 U UA U201309588U UA U201309588 U UAU201309588 U UA U201309588U UA 89399 U UA89399 U UA 89399U
Authority
UA
Ukraine
Prior art keywords
cells
microplate
cell
respiratory syndrome
porcine reproductive
Prior art date
Application number
UAU201309588U
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Николай Петрович Ситюк
Инна Васильевна Артеменко
Валерий Иванович Билокинь
Сергей Анатолиевич Нычик
Original Assignee
Институт Ветеринарной Медицины Наан Украины
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт Ветеринарной Медицины Наан Украины filed Critical Институт Ветеринарной Медицины Наан Украины
Priority to UAU201309588U priority Critical patent/UA89399U/en
Publication of UA89399U publication Critical patent/UA89399U/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

An immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome involves inoculation of sensitive culture cells onto 96-cell plastic microplate with flat bottom, dilution of test virus-containg material by ten-fold dilution technique in 96-cell plastic microplates with round bottom followed by transferring thereof into microplate cells with monolayer of cell culture, incubation in COconditions of temperature-regulated chamber for 7 days, removal of culture medium from microplate cells with washing monolayer of cell culture, addition of fixing buffer into all microplate cells, stepwise addition of working dilution of specific blood serum, working dilution of conjugate, substrate solution into all microplate cells, termination of reaction by addition of stop solution into each microplate cell and reaction recording by means of inverted light microscope wherein infected cells have the appearance of isolated cells or cell clumps with deep red colored cytoplasma and pale nucleus. The method is used for virologic diagnostics of porcine reproductive and respiratory syndrome in domestic and wild pigs by investigation of biological material (blood, suspension of organs, biological fluids) or by determination of infectious activity titer of vaccine, reference strains or field isolates of porcine reproductive and respiratory syndrome virus.
UAU201309588U 2013-07-31 2013-07-31 Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome UA89399U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UAU201309588U UA89399U (en) 2013-07-31 2013-07-31 Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UAU201309588U UA89399U (en) 2013-07-31 2013-07-31 Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome

Publications (1)

Publication Number Publication Date
UA89399U true UA89399U (en) 2014-04-25

Family

ID=52281838

Family Applications (1)

Application Number Title Priority Date Filing Date
UAU201309588U UA89399U (en) 2013-07-31 2013-07-31 Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome

Country Status (1)

Country Link
UA (1) UA89399U (en)

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