UA89399U - Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome - Google Patents
Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndromeInfo
- Publication number
- UA89399U UA89399U UAU201309588U UAU201309588U UA89399U UA 89399 U UA89399 U UA 89399U UA U201309588 U UAU201309588 U UA U201309588U UA U201309588 U UAU201309588 U UA U201309588U UA 89399 U UA89399 U UA 89399U
- Authority
- UA
- Ukraine
- Prior art keywords
- cells
- microplate
- cell
- respiratory syndrome
- porcine reproductive
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
An immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome involves inoculation of sensitive culture cells onto 96-cell plastic microplate with flat bottom, dilution of test virus-containg material by ten-fold dilution technique in 96-cell plastic microplates with round bottom followed by transferring thereof into microplate cells with monolayer of cell culture, incubation in COconditions of temperature-regulated chamber for 7 days, removal of culture medium from microplate cells with washing monolayer of cell culture, addition of fixing buffer into all microplate cells, stepwise addition of working dilution of specific blood serum, working dilution of conjugate, substrate solution into all microplate cells, termination of reaction by addition of stop solution into each microplate cell and reaction recording by means of inverted light microscope wherein infected cells have the appearance of isolated cells or cell clumps with deep red colored cytoplasma and pale nucleus. The method is used for virologic diagnostics of porcine reproductive and respiratory syndrome in domestic and wild pigs by investigation of biological material (blood, suspension of organs, biological fluids) or by determination of infectious activity titer of vaccine, reference strains or field isolates of porcine reproductive and respiratory syndrome virus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201309588U UA89399U (en) | 2013-07-31 | 2013-07-31 | Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201309588U UA89399U (en) | 2013-07-31 | 2013-07-31 | Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA89399U true UA89399U (en) | 2014-04-25 |
Family
ID=52281838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU201309588U UA89399U (en) | 2013-07-31 | 2013-07-31 | Immunoperoxidase test for virologic diagnostics of porcine reproductive and respiratory syndrome |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA89399U (en) |
-
2013
- 2013-07-31 UA UAU201309588U patent/UA89399U/en unknown
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