UA84669C2 - Теплостабильные мутанты ферментов биосинтеза крахмала - Google Patents

Теплостабильные мутанты ферментов биосинтеза крахмала

Info

Publication number
UA84669C2
UA84669C2 UA2003098455A UA2003098455A UA84669C2 UA 84669 C2 UA84669 C2 UA 84669C2 UA 2003098455 A UA2003098455 A UA 2003098455A UA 2003098455 A UA2003098455 A UA 2003098455A UA 84669 C2 UA84669 C2 UA 84669C2
Authority
UA
Ukraine
Prior art keywords
mutant
adp
glucose pyrophosphorylase
amino acid
enzyme
Prior art date
Application number
UA2003098455A
Other languages
English (en)
Ukrainian (uk)
Inventor
Кертис Л. Ханнан
Томас У. ГРИН
Браян Берджер
Original Assignee
Юніверсити Оф Флорида Рисерч Фаундейшн, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юніверсити Оф Флорида Рисерч Фаундейшн, Инк. filed Critical Юніверсити Оф Флорида Рисерч Фаундейшн, Инк.
Publication of UA84669C2 publication Critical patent/UA84669C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Заявлен полинуклеотид, кодирующий мутантную большую субъединицу полипептида растительной эндоспермной АДФ-глюкозопирофосфорилазы или ее биологически активный фрагмент. Мутантный полипептид включает аминокислотные мутации в двух или больше сайтах аминокислотной последовательности, а именно в положениях 333, 177 и 396 аминокислотной последовательности большой субъединицы дикого типа АДФ-глюкозопирофосфорилазы кукурузы. В случае экспрессии мутантного полипептида с малой субъединицей АДФ-глюкозопирофосфорилазы с образованием мутантного фермента АДФ-глюкозопирофосфорилазы, мутантный фермент или его биологически активный фрагмент демонстрирует повышенную теплостабильность и/или повышенную ферментативную активность в сравнении с ферментом АДФ-глюкозопирофосфорилазы дикого типа.
UA2003098455A 2001-03-14 2002-03-14 Теплостабильные мутанты ферментов биосинтеза крахмала UA84669C2 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27576801P 2001-03-14 2001-03-14

Publications (1)

Publication Number Publication Date
UA84669C2 true UA84669C2 (ru) 2008-11-25

Family

ID=23053711

Family Applications (1)

Application Number Title Priority Date Filing Date
UA2003098455A UA84669C2 (ru) 2001-03-14 2002-03-14 Теплостабильные мутанты ферментов биосинтеза крахмала

Country Status (17)

Country Link
US (1) US6969783B2 (ru)
EP (2) EP2107111A1 (ru)
CN (1) CN1503842A (ru)
AR (1) AR032990A1 (ru)
AT (1) ATE430192T1 (ru)
AU (1) AU2002305047A1 (ru)
BR (1) BR0208079A (ru)
CA (1) CA2441207A1 (ru)
DE (1) DE60232138D1 (ru)
DK (1) DK1373488T3 (ru)
ES (1) ES2325984T3 (ru)
MX (1) MXPA03008350A (ru)
PT (1) PT1373488E (ru)
RU (1) RU2303633C2 (ru)
UA (1) UA84669C2 (ru)
WO (1) WO2002072784A2 (ru)
ZA (1) ZA200306963B (ru)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6809235B2 (en) * 1996-11-18 2004-10-26 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
AU3993299A (en) 1998-05-14 1999-11-29 University Of Florida Heat stable mutants of starch biosynthesis enzymes
WO2005019425A2 (en) 2003-08-18 2005-03-03 University Of Florida Research Foundation, Inc. Heat stable variants of adenosine diphosphate glucose pyrophosphorylase
US9074193B2 (en) * 2008-04-09 2015-07-07 University Of Florida Research Foundation, Inc. Heat resistant plants and plant tissues and methods and materials for making and using same
US8362321B2 (en) * 2009-02-02 2013-01-29 University Of Florida Research Foundation, Inc. Methods and materials for increasing starch biosynthesis in plants
US20160194652A1 (en) * 2013-08-14 2016-07-07 Kansas State University Research Foundation Expression of thermostable starch synthase genes improves the yield in heat stress
CN106459933B (zh) * 2014-05-19 2021-05-18 佛罗里达大学研究基金公司 用于增加谷物产量的方法
CN106337056B (zh) * 2016-09-23 2018-10-02 中国热带农业科学院热带生物技术研究所 一种改良植物果实支链淀粉品质的基因及其编码产物与应用
CN108642199B (zh) * 2018-04-16 2021-12-21 张家口市农业科学院 与谷子旗叶长性状相关的snp标记及其检测引物和应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2002A (en) * 1841-03-12 Tor and planter for plowing
US6013861A (en) 1989-05-26 2000-01-11 Zeneca Limited Plants and processes for obtaining them
DE4013144A1 (de) 1990-04-20 1991-10-24 Inst Genbiologische Forschung Neue plasmide, enthaltend dna-sequenzen, die veraenderungen der karbohydrat- und proteinkonzentration und der karbohydrat- und proteinzusammensetzung in kartoffelknollen hervorrufen, sowie zellen einer kartoffelpflanze, enthaltend diese plasmide
JP3234598B2 (ja) 1990-11-23 2001-12-04 プラント・ジエネテイツク・システムズ・エヌ・ベー 単子葉植物の形質転換方法
EP0664835B1 (en) 1992-10-14 2004-05-19 Syngenta Limited Novel plants and processes for obtaining them
GB9223454D0 (en) 1992-11-09 1992-12-23 Ici Plc Novel plants and processes for obtaining them
GB9307408D0 (en) 1993-04-08 1993-06-02 Danisco Transgenic plants
US5498831A (en) 1993-07-23 1996-03-12 Dna Plant Technology Corporation Pea ADP-glucose pyrophosphorylase subunit genes and their uses
GB9412018D0 (en) 1994-06-16 1994-08-03 Cambridge Advanced Tech Modification of starch content in plants
US5872216A (en) 1994-09-01 1999-02-16 University Of Florida Research Foundation, Inc. Materials and methods for increasing corn seed weight
US5589618A (en) 1994-09-01 1996-12-31 University Of Florida Materials and methods for increasing corn seed weight
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
US6403863B1 (en) 1996-11-18 2002-06-11 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
BR9712771A (pt) 1996-11-18 1999-10-26 Univ Florida Mutantes estáveis ao calor de enzimas de biossìntese de amido
US6809235B2 (en) 1996-11-18 2004-10-26 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
AU3993299A (en) 1998-05-14 1999-11-29 University Of Florida Heat stable mutants of starch biosynthesis enzymes
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6013881A (en) * 1999-03-30 2000-01-11 Honeywell Inc. Key operated safety interlock switch
US6378968B1 (en) * 1999-06-07 2002-04-30 Kuo-Chan Weng Sliding track assembly
AR035395A1 (es) 2000-03-01 2004-05-26 Res & Dev I Inc Plantas transgenicas que presentan un mayor rendimiento en semillas, una mayor biomasa y un mayor indice de cosecha

Also Published As

Publication number Publication date
DE60232138D1 (de) 2009-06-10
ZA200306963B (en) 2004-05-03
WO2002072784A2 (en) 2002-09-19
MXPA03008350A (es) 2004-10-15
DK1373488T3 (da) 2009-08-17
EP1373488A2 (en) 2004-01-02
WO2002072784A3 (en) 2003-08-07
EP2107111A1 (en) 2009-10-07
RU2003130272A (ru) 2005-04-20
AU2002305047A1 (en) 2002-09-24
ES2325984T3 (es) 2009-09-28
CA2441207A1 (en) 2002-09-19
RU2303633C2 (ru) 2007-07-27
US6969783B2 (en) 2005-11-29
CN1503842A (zh) 2004-06-09
EP1373488B1 (en) 2009-04-29
PT1373488E (pt) 2009-07-31
ATE430192T1 (de) 2009-05-15
BR0208079A (pt) 2004-07-27
EP1373488A4 (en) 2004-09-15
AR032990A1 (es) 2003-12-03
US20030056248A1 (en) 2003-03-20

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