UA50339A - Method for production of lactoferrine concentrate and cow milk protein containing copper - Google Patents

Method for production of lactoferrine concentrate and cow milk protein containing copper

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Publication number
UA50339A
UA50339A UA2001128953A UA2001128953A UA50339A UA 50339 A UA50339 A UA 50339A UA 2001128953 A UA2001128953 A UA 2001128953A UA 2001128953 A UA2001128953 A UA 2001128953A UA 50339 A UA50339 A UA 50339A
Authority
UA
Ukraine
Prior art keywords
lactoferrine
containing copper
protein containing
temperature
during
Prior art date
Application number
UA2001128953A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Валентина Дмитріївна Отт
Валентина Дмитриевна Отт
Світлана Володимирівна Дюкарева
Светлана Владимировна Дюкарева
Олег Ростиславович Мельников
Original Assignee
Інститут Педіатрії, Акушерства Та Гінекології Амн України
Институт Педиатрии, Акушерства И Гинекологии Амн Украины
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Інститут Педіатрії, Акушерства Та Гінекології Амн України, Институт Педиатрии, Акушерства И Гинекологии Амн Украины filed Critical Інститут Педіатрії, Акушерства Та Гінекології Амн України
Priority to UA2001128953A priority Critical patent/UA50339A/en
Publication of UA50339A publication Critical patent/UA50339A/en

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Abstract

Method for production of lactoferrine concentrate and cow milk protein containing copper, at which the mild is separated into fats and milk plasma by centrifugation at 5000 g during 20-25 minutes at the temperature of 3-5°C. Casein is removed from milk plasma by acidification method 1 M HCl up to the isoelectric point of protein pH 4.6 followed by incubation during 18-24 minutes at the temperature of 23-26 °C and centrifugation during 1 hour at 19000 g and at the temperature of 2-4°C. From lactoserum (supernatant) after adding of alkali 1 M NaOH up to pH 7.2 by solution of ammonium sulphate in the range of saturation 30-80 per cent a fraction is precipitated in which is lactoferrine and protein containing copper. Obtained deposit is dissolved in distilled water and pasteurized at the temperature of 55-65 °C during 30 minutes in order to make microbiological purification of preparation. Water dialysis is made followed by vacuum lyophilization. Amount of lactoferrine and protein containing copper in volume unit of lyophilizate is determined by electrophoresis method in polyacrylamide gel with sodium dodecylsulphate. Obtained concentrate of lactoferrine and protein containing copper is subject to microbiological analysis for pathogenic flora.
UA2001128953A 2001-12-24 2001-12-24 Method for production of lactoferrine concentrate and cow milk protein containing copper UA50339A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UA2001128953A UA50339A (en) 2001-12-24 2001-12-24 Method for production of lactoferrine concentrate and cow milk protein containing copper

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UA2001128953A UA50339A (en) 2001-12-24 2001-12-24 Method for production of lactoferrine concentrate and cow milk protein containing copper

Publications (1)

Publication Number Publication Date
UA50339A true UA50339A (en) 2002-10-15

Family

ID=74235684

Family Applications (1)

Application Number Title Priority Date Filing Date
UA2001128953A UA50339A (en) 2001-12-24 2001-12-24 Method for production of lactoferrine concentrate and cow milk protein containing copper

Country Status (1)

Country Link
UA (1) UA50339A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2763552C1 (en) * 2021-05-31 2021-12-30 Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО Астраханский ГМУ Минздрава России) Method for isolation of camel lactoferrin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2763552C1 (en) * 2021-05-31 2021-12-30 Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО Астраханский ГМУ Минздрава России) Method for isolation of camel lactoferrin

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