RO103682B1 - Isolation method of human ceruloplasmin - Google Patents
Isolation method of human ceruloplasminInfo
- Publication number
- RO103682B1 RO103682B1 RO137849A RO13784989A RO103682B1 RO 103682 B1 RO103682 B1 RO 103682B1 RO 137849 A RO137849 A RO 137849A RO 13784989 A RO13784989 A RO 13784989A RO 103682 B1 RO103682 B1 RO 103682B1
- Authority
- RO
- Romania
- Prior art keywords
- isolation method
- human ceruloplasmin
- human
- ceruloplasmin
- chromatographing
- Prior art date
Links
- 101000908019 Homo sapiens Ceruloplasmin Proteins 0.000 title 1
- 238000002955 isolation Methods 0.000 title 1
- 229920002271 DEAE-Sepharose Polymers 0.000 abstract 1
- RHMFKDJJAZWJLR-UHFFFAOYSA-J [O-]P([O-])(=O)OP(=O)([O-])[O-].[Na+].C(C)(=O)O.[Na+].[Na+].[Na+] Chemical compound [O-]P([O-])(=O)OP(=O)([O-])[O-].[Na+].C(C)(=O)O.[Na+].[Na+].[Na+] RHMFKDJJAZWJLR-UHFFFAOYSA-J 0.000 abstract 1
- YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 abstract 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Abstract
Procedeul conform inventiei consta în aceea ca serul sau plasma umana se trateaza cu solutie CuSO4 1 M (100:0,4) si apoi se mentine la 68 degree C, timp de 30 min, se centrifugheaza, se cromatografiaza întâi pe DEAE-Sepharose CL-68 în tampon 0,02 M pirofosfat de sodiu - acid acetic pH=7,5 si apoi pe Cibacron - Blue F3GA - Bio_gel A5 m în TFS 0,05 M pH=7.The process according to the invention consists in treating the human serum or plasma with 1 M CuSO4 solution (100: 0.4) and then at 68 DEG C. for 30 minutes, centrifuging, first chromatographing on DEAE-Sepharose CL -68 in buffer 0.02 M sodium pyrophosphate-acetic acid pH = 7.5 and then Cibacron-Blue F3GA-Bio_gel A5 m in TMS 0.05 M pH = 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO137849A RO103682B1 (en) | 1989-01-20 | 1989-01-20 | Isolation method of human ceruloplasmin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO137849A RO103682B1 (en) | 1989-01-20 | 1989-01-20 | Isolation method of human ceruloplasmin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO103682A2 RO103682A2 (en) | 1991-12-09 |
| RO103682B1 true RO103682B1 (en) | 1992-03-26 |
Family
ID=20123823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO137849A RO103682B1 (en) | 1989-01-20 | 1989-01-20 | Isolation method of human ceruloplasmin |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO103682B1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6964764B2 (en) | 1999-11-13 | 2005-11-15 | Talecris Biotherapeutics, Inc. | Method of thrombolysis by local delivery of reversibly inactivated acidified plasmin |
| US6969515B2 (en) | 1999-11-13 | 2005-11-29 | Talecris Biotherapeutics, Inc. | Method of thrombolysis by local delivery of reversibly inactivated acidified plasmin |
| US6355243B1 (en) | 1999-11-13 | 2002-03-12 | Bayer Corporation | Method of thrombolysis by local delivery of active plasmin |
| US7544500B2 (en) | 1999-11-13 | 2009-06-09 | Talecris Biotherapeutics, Inc. | Process for the production of a reversibly inactive acidified plasmin composition |
-
1989
- 1989-01-20 RO RO137849A patent/RO103682B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO103682A2 (en) | 1991-12-09 |
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