UA28003C2 - Chimeric polypeptide having piruvate ortophosphate activity and increases plant cold-resistance, DNA Fragment , recombinant vector, a method for obtaining polypeptide - Google Patents

Chimeric polypeptide having piruvate ortophosphate activity and increases plant cold-resistance, DNA Fragment , recombinant vector, a method for obtaining polypeptide

Info

Publication number
UA28003C2
UA28003C2 UA96031191A UA96031191A UA28003C2 UA 28003 C2 UA28003 C2 UA 28003C2 UA 96031191 A UA96031191 A UA 96031191A UA 96031191 A UA96031191 A UA 96031191A UA 28003 C2 UA28003 C2 UA 28003C2
Authority
UA
Ukraine
Prior art keywords
polypeptide
activity
recombinant vector
resistance
amino acid
Prior art date
Application number
UA96031191A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Схозо ОХТА
Сатору УСАМІ
Сатору Усами
Джеймс Нігель БУРНЕЛЛ
Джеймс Нигель БУРНЕЛЛ
Original Assignee
Джапан Тобако Інк
Джапан Тобако Инк
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/JP1994/002022 external-priority patent/WO1995015385A1/en
Application filed by Джапан Тобако Інк, Джапан Тобако Инк filed Critical Джапан Тобако Інк
Publication of UA28003C2 publication Critical patent/UA28003C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1294—Phosphotransferases with paired acceptors (2.7.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This invention relates to a new cold-resistant salt or ester of piruvic acid, coding cloned DNA of the same and recombinant vector containing DNA as agent for providing cold resistance of plants. It is proposed a method for creating cold-resistant salt or ester of piruvic acid , orthophosphate dikinase which has amino acid sequence, which is the same as amino acid sequence of 1/6 of all the field from C-ending the next polypeptide. Salt or ester of piruvic acid, orthophosphate dikinase have amino acid sequence. Polypeptide has amino acid sequence, which has homology of not less than 50 % with amino acid sequence. The said polypeptide has cold-resistant salt or ester of piruvic acid, activity of orthophosphate dikinase.
UA96031191A 1994-07-29 1995-05-30 Chimeric polypeptide having piruvate ortophosphate activity and increases plant cold-resistance, DNA Fragment , recombinant vector, a method for obtaining polypeptide UA28003C2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP19778094 1994-07-29
PCT/JP1994/002022 WO1995015385A1 (en) 1993-12-03 1994-12-01 Polypeptide having cold-resistant pyruvate phosphate dikinase activity, dna coding for the same, and recombinant vector and transformed plant both containing said dna
PCT/JP1995/001040 WO1996004369A1 (en) 1994-07-29 1995-05-30 Polypeptide having cold-resistant pyruvate phosphate dikinase activity, dna coding for the polypeptide, recombinant vector containing the dna, and transformed plant

Publications (1)

Publication Number Publication Date
UA28003C2 true UA28003C2 (en) 2000-10-16

Family

ID=16380231

Family Applications (1)

Application Number Title Priority Date Filing Date
UA96031191A UA28003C2 (en) 1994-07-29 1995-05-30 Chimeric polypeptide having piruvate ortophosphate activity and increases plant cold-resistance, DNA Fragment , recombinant vector, a method for obtaining polypeptide

Country Status (6)

Country Link
CN (1) CN1174093C (en)
BR (1) BR9506291A (en)
RO (1) RO118451B1 (en)
RU (1) RU2136748C1 (en)
UA (1) UA28003C2 (en)
WO (1) WO1996004369A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0690128A4 (en) * 1993-12-03 2004-03-03 Japan Tobacco Inc Polypeptide having cold-resistant pyruvate phosphate dikinase activity, dna coding for the same, and recombinant vector and transformed plant both containing said dna
CN102653727A (en) * 2012-01-18 2012-09-05 江南大学 Construction method and application of pyruvic acid-phosphoric acid double-kinase recombinant expression strain

Also Published As

Publication number Publication date
WO1996004369A1 (en) 1996-02-15
CN1174093C (en) 2004-11-03
BR9506291A (en) 1997-08-05
RO118451B1 (en) 2003-05-30
MX9601212A (en) 1997-09-30
CN1135770A (en) 1996-11-13
RU2136748C1 (en) 1999-09-10

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