UA26668U - Method for identifying raw products (flowers, roots, or leaves) of medical dandelion - Google Patents

Abstract

The proposed method for identifying raw products (flowers, roots, or leaves) of medical dandelion consists in determining the presence and the content of chicory acid in the flowers, roots, or leaves.

Description

Description of the invention

A useful model belongs to the field of pharmacy, in particular to phytochemistry and can be used for 2 standardization of medicinal plant raw materials.

It is known that the raw materials of dandelion have been widely used in phytotherapy for a long time, both in the form of monopreparations and in the form of constituent parts of multicomponent phytocompositions |11.

There is a known method of identifying dandelion flowers based on the presence of the flavonoid luteolin in the plant material, using the TLC method |21. 70 The closest analogue is the process of identifying dandelion flowers by the presence of luteolin, which is carried out using the TLC method |21.

According to the closest analogue, the identification of luteolin is carried out by the TLC method. A chromatographic plate "KiezeIdeI-6O E254, size 10 cmx15 cm, mobile phase benzene-ethyl acetate-formic acid (40:10:5 ml) is used. Spots are developed using a borate-lemon mixture (21.

A significant drawback of the existing method of identification of raw materials is that, according to the method of the closest analogue, it is possible to determine the biologically active substance luteolin only in flowers for which this component is specific.

The object to be improved is the process of identification and determination of the content of biologically active substances in raw materials (flowers, leaves, roots) of dandelion. The known definition cannot be used for all parts of the plant, as it cannot confirm the presence of the main biologically active substances in other organs of the dandelion, in particular in the leaf and root, and does not provide quantitative characteristics of BAR content in raw materials.

The nature of the improvement made to the object. According to the literature, all parts of the dandelion (roots, leaves, flowers) largely contain oxycinnamic acid derivatives, in particular, the biologically active 29 substance chicory acid, the content of which in the dandelion is quite high |Z). The process of identification and quantitative determination of chicory acid in raw materials of dandelion (roots, leaves, flowers) consists in finding conditions for chromatographic separation of chicory acid from other oxycinnamic acids. The useful model "Method of identification of raw materials (roots, leaves, flowers) of dandelion" is based on the task of improving the identification of raw materials of dandelion (roots, leaves, flowers) by confirming the presence of chicory acid and determining its content, and thereby ensuring ( Se) the possibility of standardization of raw materials.

The task is solved by the fact that, according to a useful model, the identification and quantitative determination of chicory acid using the method of high-performance liquid chromatography (HPLC) is used in the presence of other derivatives of oxycinnamic acid, with preliminary purification from interfering substances

From the chromatography of substances. high school

Example: 1 g (exact weight) of the researched crushed raw material was placed in a conical flask equipped with a reflux condenser, 100 ml of a mixture of methanol - water (70:30) was added and kept on a boiling water heater for 15 minutes. The extract was then cooled to room temperature and filtered through a red ribbon filter. An amount of water was added to 5 ml of the resulting solution so that the concentration of methanol in Z7Z 70 was 2095, and the resulting sample was passed through a pre-activated bml with methanol and a washed 1 Oml of water cartridge for solid-phase extraction "Zyregsieap Is-148 5RE Tybez 1ti" produced by the company Zireiso (USA). The cartridge was washed with ZOml 2095 methanol solution. The resulting solution was evaporated to dryness in a vacuum, and the dry residue was dissolved in 5 ml of 7095 methanol, filtered through a filter with a pore diameter of 0.45 μm. 15 bmcl of the studied solution and the chicory acid standard solution were alternately chromatographed under the following conditions: C18 X-Teith column, size 250mmx4, bmm, particle size 5μm; mobile phase: acetonitrile - (o) water - acetic acid (20:77:3); the temperature of the column is 302C, the wavelength of detection is ZZOnm; flow rate

Fu mobile phase ml/min.

The content of chicory acid in the studied raw material (in 95) is calculated according to the following formula:

FU o m Av "Sy 001 00100 so Ast zhar k00- M de Arr - the plane of the peak of chicory acid on the chromatogram of the studied solution;

Ast - the plane of the chicory acid peak on the chromatogram of the standard chicory acid solution;

Set - concentration of chicory acid in a standard solution of chicory acid, g/ml; c Tpr - weight of the studied raw material, g;

MU - mass loss during drying in the studied raw material.

Preparation of a standard solution of chicory acid: 0.010 g of a reliable standard of chicory acid is placed in a volumetric flask with a capacity of 25 ml, dissolved in 30 ml of a methanol-water mixture (70:30), brought to the mark with the same mixture and mixed. ml of the obtained solution is placed in a volumetric flask with a capacity of 50 ml, brought up to the mark with a methanol-water mixture (70:30) and mixed.

There should be a peak on the chromatogram of the tested solution, the time of which corresponds to the time of the peak of chicory acid on the chromatogram of the standard solution of chicory acid (Fig. 1-4).

Based on experimental data, we recommend the following maximum content of chicory acid for dandelion raw materials: for leaves not less than 195, for flowers not less than 0.595, and for roots not less than 0.295 in terms of dried raw materials.

The useful model makes it possible to identify and determine the quantitative content of dandelion raw materials (roots, leaves, flowers) according to the presence and content of chicory acid as a physiologically active component present in all parts of dandelion. A useful model contributes to the improvement of the quality of promising raw materials. A comparison of the dandelion identification process in the prototype and the invention is shown in the table.

Characteristics of the dandelion standardization process 76 1. Closest analogue of Luteolin Flower 1. TLC method - qualitative specific determination. 2. Possibility of semi-quantitative standardization of raw materials. 2 Invention Chicory acid Flowers, leaves, roots 1. HPLC method - qualitative specific determination. 2. The possibility of quantitative standardization of raw materials

References: 1) Medicinal plants: Encyclopedic reference / Resp. ed. A.M. Grodzinsky - Kyiv: Head, editor. URE, -1989.-544 p.: ill. 2) Pat. Mo16657 OA, MPK B01M 30/02 / O. O. Tsurkan, T. V. Kovalchuk, S. A. Shklyaev, A. V. Gudzenko /

Mo200602243; Application 01.03.2006; Imprint 15.08.2006. The method of identification of dandelion flowers.

Z) UMPSHate S. A., Soidziope RG., Sgeeppat I. Riamopoidv, sippatis asiiy apa sostagipz iot (She ayegepi ivvce apa teaisipa! rgeragayopz oi Tagahasit oiPisipa|e. // Rpuospetivigu. - 1996. - 42 (1). - R.121-127.

Claims (1)

The formula of the invention is the method of identifying raw materials of dandelion (flowers, roots, leaves), which includes the determination of the presence and content of biologically active substances, which differs in that the presence and content of chicory acid is determined by the method of high-performance liquid chromatography (HPLC) in the presence of other oxycinnamic acids . (ee) (Se) (Se) (Se) p - and? name) (o) (o) (o) IR e) 60 b5