CN106198832B - A kind of quality of production control method of intensified loquet distillate - Google Patents

A kind of quality of production control method of intensified loquet distillate Download PDF

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CN106198832B
CN106198832B CN201610481241.1A CN201610481241A CN106198832B CN 106198832 B CN106198832 B CN 106198832B CN 201610481241 A CN201610481241 A CN 201610481241A CN 106198832 B CN106198832 B CN 106198832B
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廖展苇
丘伟业
周凯华
陈辉
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Guangxi Gehongtang Pharmaceutical Co ltd
Jinji Pharmaceutical Co ltd
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LINGFENG PHARMACEUTICAL CO Ltd GUANGXI
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
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    • G01N2030/027—Liquid chromatography

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Abstract

The invention discloses a kind of quality of production control method of intensified loquet distillate, this method includes：Production control method, method of quality control.This method is respectively adopted production control method, discrimination method, content detection and carries out quality of production control to intensified loquet distillate, and it has the characteristics that, and stability is strong, measurement is accurate, speed is fast, more comprehensively, is a kind of excellent intensified loquet distillate quality of production control method.

Description

A kind of quality of production control method of intensified loquet distillate
Technical field
The present invention relates to medicine quality of production detection technique field, and in particular to a kind of quality of production control of intensified loquet distillate Method processed.
Background technology
Intensified loquet distillate main component is：Loquat leaf, pappy shell, the tuber of stemona, Cynanchum glaucescens, the root bark of white mulberry, balloonflower root, menthol.Auxiliary material For sodium benzoate, Steviosin, citric acid, flavoring pineapple essence, it is mainly used in yin-nourishing and astringes the lung, cough-relieving apophlegmatic is used for bronchitis cough, Chinese medicines quasi-word Z45020167
The content of the invention
It is an object of the invention to provide a kind of quality of production control method of intensified loquet distillate, so as to more comprehensive The accurately quality of production, detection and control intensified loquet distillate.
The present invention is achieved by the following technical programs：
A kind of quality of production control method of intensified loquet distillate：This method includes production control method, method of quality control；
Production control method：
Loguat leaf 69g, pappy shell 50g, tuber of stemona 15g, Cynanchum glaucescens 9g, root bark of white mulberry 6g, balloonflower root 6g is taken to add water to cook three times, every time 1.5h, collecting decoction, filtration, about 750ml is concentrated into, adds sodium benzoate 2.5g, is stirred to dissolve, adds sucrose 600g, continue to add Heat is kept for 20 minutes to boiling, and is stood, filtration, is added citric acid 0.5g, the essence and menthol 0.15g that are dissolved with ethanol, is stirred Mix, mix, stand, add water to 1000ml, mix, produce；
Method of quality control：
A, discrimination method：Intensified loquet distillate 80ml is taken, is extracted 2 times, each 50ml with the n-butanol shaking of ammonia solution saturation, Merge n-butanol extracting liquid, washed with the water 50ml of n-butanol saturation, discard water lotion, divide and take n-butanol layer, be evaporated, residue adds Ethanol 1ml makes dissolving, as need testing solution；Loguat leaf control medicinal material 2g is taken, adds water 50ml, is heated to reflux 1h, let cool, use cotton Flower filtration, takes filtrate, with the n-butanol shaking extraction 2 times, each 30ml of ammonia solution saturation, is merging the same of n-butanol extracting liquid When, using being made in the same way of loguat leaf control medicinal material solution；Cynanchum glaucescens control medicinal material 1g is taken, is made in the same way of Cynanchum glaucescens control medicinal material solution； Above-mentioned each 4-8 μ l of three kinds of solution are drawn according to thin-layered chromatography, are put respectively on same silica gel g thin-layer plate, with 5：2 toluene- Acetone is solvent, is deployed, and takes out, dries, be placed under 365nm ultraviolet lamp and inspect；In test sample chromatogram, with Cynanchum glaucescens pair According on the corresponding position of medicinal material chromatogram, show the fluorescence spot of same color；Spray is added with 20% phosphomolybdic acid ethanol solution at 105 DEG C Heat is clear to spot development, is inspected under daylight, in test sample chromatogram, on position corresponding with loguat leaf control medicinal material chromatogram, The spot of aobvious same color；Pappy shell control medicinal material 2g is taken, is made in the same way of control medicinal material solution.Tested according to thin-layered chromatography, Cynanchum glaucescens control medicinal material solution and each 4-8 μ l of pappy shell medicinal material solution are taken, is put respectively on same silica gel g thin-layer plate, with 5：2 first Benzene-acetone is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；In test sample chromatogram, with comparison medicine wood color Compose on corresponding position, show the spot of same color；
B, content detection：It is measured according to high performance liquid chromatography, including it is chromatographic condition and system suitability, right The preparation of preparation, need testing solution according to product solution, measure.
In the step b chromatographic condition and system suitability be using octyl silane group silica gel as filler, with Acetonitrile -0.01mol/L dipotassium hydrogen phosphate solution -0.005mol/L heptane sulfonic acid sodium salts are according to 20：40：40 volume ratio is stream Dynamic phase, Detection wavelength 220nm, number of theoretical plate calculate >=2000 by morphine peak.
The preparation of reference substance solution is to take morphine reference substance is accurately weighed to put in brown measuring bottle in the step b, is added containing 5% The solution that every 1ml contains 24 μ g is made in 20% methanol solution of acetic acid, produces.
The preparation of need testing solution is that precision measures intensified loquet distillate 3ml in the step b, is put in 10ml brown measuring bottles, 20% methanol solution containing 5% acetic acid is added to shake up to scale, filtered, produced with 0.45 μm of miillpore filter.
Measure is that precision draws reference substance solution and each 10 μ l of need testing solution respectively in the step b, injects liquid phase color Spectrometer, determine and produce.
The intensified loquet distillate per 1ml containing pappy shell in terms of morphine >=15 μ g.
The beneficial effects of the present invention are：Production control method, discrimination method, content detection is respectively adopted to strong in this method Power loquat dew carries out quality of production control, and it has the characteristics that, and stability is strong, measurement is accurate, speed is fast, more comprehensively, is a kind of excellent Intensified loquet distillate quality of production control method.
Embodiment
Technical scheme is further described below in conjunction with specific embodiment, but claimed scope is not It is confined to described.
Embodiment one
A kind of quality of production control method of intensified loquet distillate：This method includes production control method, method of quality control；
Production control method：
Loguat leaf 69g, pappy shell 50g, tuber of stemona 15g, Cynanchum glaucescens 9g, root bark of white mulberry 6g, balloonflower root 6g is taken to add water to cook three times, every time 1.5h, collecting decoction, filtration, about 750ml is concentrated into, adds sodium benzoate 2.5g, is stirred to dissolve, adds sucrose 600g, continue to add Heat is kept for 20 minutes to boiling, and is stood, filtration, is added citric acid 0.5g, the essence and menthol 0.15g that are dissolved with ethanol, is stirred Mix, mix, stand, add water to 1000ml, mix, produce；
Method of quality control：
A, discrimination method：Intensified loquet distillate 80ml is taken, is extracted 2 times, each 50ml with the n-butanol shaking of ammonia solution saturation, Merge n-butanol extracting liquid, washed with the water 50ml of n-butanol saturation, discard water lotion, divide and take n-butanol layer, be evaporated, residue adds Ethanol 1ml makes dissolving, as need testing solution；Loguat leaf control medicinal material 2g is taken, adds water 50ml, is heated to reflux 1h, let cool, use cotton Flower filtration, takes filtrate, with the n-butanol shaking extraction 2 times, each 30ml of ammonia solution saturation, is merging the same of n-butanol extracting liquid When, using being made in the same way of loguat leaf control medicinal material solution；Cynanchum glaucescens control medicinal material 1g is taken, is made in the same way of Cynanchum glaucescens control medicinal material solution； Above-mentioned each 4 μ l of three kinds of solution are drawn according to thin-layered chromatography, are put respectively on same silica gel g thin-layer plate, with 5：2 toluene-the third Ketone is solvent, is deployed, and takes out, dries, be placed under 365nm ultraviolet lamp and inspect；In test sample chromatogram, compareed with Cynanchum glaucescens On the corresponding position of medicinal material chromatogram, show the fluorescence spot of same color；Spray is with 20% phosphomolybdic acid ethanol solution, in 105 DEG C of heating It is clear to spot development, inspect under daylight, in test sample chromatogram, on position corresponding with loguat leaf control medicinal material chromatogram, show The spot of same color；Pappy shell control medicinal material 2g is taken, is made in the same way of control medicinal material solution.Test, take according to thin-layered chromatography Cynanchum glaucescens control medicinal material solution and each 4 μ l of pappy shell medicinal material solution, put respectively on same silica gel g thin-layer plate, with 5：2 toluene- Acetone is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；In test sample chromatogram, with control medicinal material chromatogram On corresponding position, show the spot of same color；
B, content detection：It is measured according to high performance liquid chromatography, chromatographic condition and system suitability：With octane Base silane bonded silica gel is filler, molten with acetonitrile -0.01mol/L dipotassium hydrogen phosphate solution -0.005mol/L sodium heptanesulfonates Liquid is according to 20：40：40 volume ratio is mobile phase, and Detection wavelength 220nm, number of theoretical plate is by morphine peak calculating >=2000；It is right According to the preparation of product solution；Take morphine reference substance is accurately weighed to put in brown measuring bottle, add 20% methanol solution containing 5% acetic acid to be made Contain 24 μ g solution per 1ml, produce；The preparation of need testing solution：Precision measures intensified loquet distillate 3ml, puts 10ml brown measuring bottles In, add 20% methanol solution containing 5% acetic acid to shake up to scale, filtered, produced with 0.45 μm of miillpore filter；Measure：Essence respectively Close absorption reference substance solution and each 10 μ l of need testing solution, inject liquid chromatograph, determine and produce, intensified loquet distillate contains per 1ml Pappy shell in terms of morphine >=15 μ g.
Embodiment two
A kind of quality of production control method of intensified loquet distillate：This method includes production control method, method of quality control；
Production control method：
Loguat leaf 69g, pappy shell 50g, tuber of stemona 15g, Cynanchum glaucescens 9g, root bark of white mulberry 6g, balloonflower root 6g is taken to add water to cook three times, every time 1.5h, collecting decoction, filtration, about 750ml is concentrated into, adds sodium benzoate 2.5g, is stirred to dissolve, adds sucrose 600g, continue to add Heat is kept for 20 minutes to boiling, and is stood, filtration, is added citric acid 0.5g, the essence and menthol 0.15g that are dissolved with ethanol, is stirred Mix, mix, stand, add water to 1000ml, mix, produce；
Method of quality control：
A, discrimination method：Intensified loquet distillate 80ml is taken, is extracted 2 times, each 50ml with the n-butanol shaking of ammonia solution saturation, Merge n-butanol extracting liquid, washed with the water 50ml of n-butanol saturation, discard water lotion, divide and take n-butanol layer, be evaporated, residue adds Ethanol 1ml makes dissolving, as need testing solution；Loguat leaf control medicinal material 2g is taken, adds water 50ml, is heated to reflux 1h, let cool, use cotton Flower filtration, takes filtrate, with the n-butanol shaking extraction 2 times, each 30ml of ammonia solution saturation, is merging the same of n-butanol extracting liquid When, using being made in the same way of loguat leaf control medicinal material solution；Cynanchum glaucescens control medicinal material 1g is taken, is made in the same way of Cynanchum glaucescens control medicinal material solution； Above-mentioned each 6 μ l of three kinds of solution are drawn according to thin-layered chromatography, are put respectively on same silica gel g thin-layer plate, with 5：2 toluene-the third Ketone is solvent, is deployed, and takes out, dries, be placed under 365nm ultraviolet lamp and inspect；In test sample chromatogram, compareed with Cynanchum glaucescens On the corresponding position of medicinal material chromatogram, show the fluorescence spot of same color；Spray is with 20% phosphomolybdic acid ethanol solution, in 105 DEG C of heating It is clear to spot development, inspect under daylight, in test sample chromatogram, on position corresponding with loguat leaf control medicinal material chromatogram, show The spot of same color；Pappy shell control medicinal material 2g is taken, is made in the same way of control medicinal material solution.Test, take according to thin-layered chromatography Cynanchum glaucescens control medicinal material solution and each 6 μ l of pappy shell medicinal material solution, put respectively on same silica gel g thin-layer plate, with 5：2 toluene- Acetone is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；In test sample chromatogram, with control medicinal material chromatogram On corresponding position, show the spot of same color；
B, content detection：It is measured according to high performance liquid chromatography, chromatographic condition and system suitability：With octane Base silane bonded silica gel is filler, molten with acetonitrile -0.01mol/L dipotassium hydrogen phosphate solution -0.005mol/L sodium heptanesulfonates Liquid is according to 20：40：40 volume ratio is mobile phase, and Detection wavelength 220nm, number of theoretical plate is by morphine peak calculating >=2000；It is right According to the preparation of product solution；Take morphine reference substance is accurately weighed to put in brown measuring bottle, add 20% methanol solution containing 5% acetic acid to be made Contain 24 μ g solution per 1ml, produce；The preparation of need testing solution：Precision measures intensified loquet distillate 3ml, puts 10ml brown measuring bottles In, add 20% methanol solution containing 5% acetic acid to shake up to scale, filtered, produced with 0.45 μm of miillpore filter；Measure：Essence respectively Close absorption reference substance solution and each 10 μ l of need testing solution, inject liquid chromatograph, determine and produce, intensified loquet distillate contains per 1ml Pappy shell in terms of morphine >=15 μ g.
Embodiment three
A kind of quality of production control method of intensified loquet distillate：This method includes production control method, method of quality control；
Production control method：
Loguat leaf 69g, pappy shell 50g, tuber of stemona 15g, Cynanchum glaucescens 9g, root bark of white mulberry 6g, balloonflower root 6g is taken to add water to cook three times, every time 1.5h, collecting decoction, filtration, about 750ml is concentrated into, adds sodium benzoate 2.5g, is stirred to dissolve, adds sucrose 600g, continue to add Heat is kept for 20 minutes to boiling, and is stood, filtration, is added citric acid 0.5g, the essence and menthol 0.15g that are dissolved with ethanol, is stirred Mix, mix, stand, add water to 1000ml, mix, produce；
Method of quality control：
A, discrimination method：Intensified loquet distillate 80ml is taken, is extracted 2 times, each 50ml with the n-butanol shaking of ammonia solution saturation, Merge n-butanol extracting liquid, washed with the water 50ml of n-butanol saturation, discard water lotion, divide and take n-butanol layer, be evaporated, residue adds Ethanol 1ml makes dissolving, as need testing solution；Loguat leaf control medicinal material 2g is taken, adds water 50ml, is heated to reflux 1h, let cool, use cotton Flower filtration, takes filtrate, with the n-butanol shaking extraction 2 times, each 30ml of ammonia solution saturation, is merging the same of n-butanol extracting liquid When, using being made in the same way of loguat leaf control medicinal material solution；Cynanchum glaucescens control medicinal material 1g is taken, is made in the same way of Cynanchum glaucescens control medicinal material solution； Above-mentioned each 8 μ l of three kinds of solution are drawn according to thin-layered chromatography, are put respectively on same silica gel g thin-layer plate, with 5：2 toluene-the third Ketone is solvent, is deployed, and takes out, dries, be placed under 365nm ultraviolet lamp and inspect；In test sample chromatogram, compareed with Cynanchum glaucescens On the corresponding position of medicinal material chromatogram, show the fluorescence spot of same color；Spray is with 20% phosphomolybdic acid ethanol solution, in 105 DEG C of heating It is clear to spot development, inspect under daylight, in test sample chromatogram, on position corresponding with loguat leaf control medicinal material chromatogram, show The spot of same color；Pappy shell control medicinal material 2g is taken, is made in the same way of control medicinal material solution.Test, take according to thin-layered chromatography Cynanchum glaucescens control medicinal material solution and each 8 μ l of pappy shell medicinal material solution, put respectively on same silica gel g thin-layer plate, with 5：2 toluene- Acetone is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；In test sample chromatogram, with control medicinal material chromatogram On corresponding position, show the spot of same color；
B, content detection：It is measured according to high performance liquid chromatography, chromatographic condition and system suitability：With octane Base silane bonded silica gel is filler, molten with acetonitrile -0.01mol/L dipotassium hydrogen phosphate solution -0.005mol/L sodium heptanesulfonates Liquid is according to 20：40：40 volume ratio is mobile phase, and Detection wavelength 220nm, number of theoretical plate is by morphine peak calculating >=2000；It is right According to the preparation of product solution；Take morphine reference substance is accurately weighed to put in brown measuring bottle, add 20% methanol solution containing 5% acetic acid to be made Contain 24 μ g solution per 1ml, produce；The preparation of need testing solution：Precision measures intensified loquet distillate 3ml, puts 10ml brown measuring bottles In, add 20% methanol solution containing 5% acetic acid to shake up to scale, filtered, produced with 0.45 μm of miillpore filter；Measure：Essence respectively Close absorption reference substance solution and each 10 μ l of need testing solution, inject liquid chromatograph, determine and produce, intensified loquet distillate contains per 1ml Pappy shell in terms of morphine >=15 μ g.

Claims (5)

  1. A kind of 1. quality of production control method of intensified loquet distillate, it is characterised in that：This method includes production control method, quality Control method；
    Production control method：
    Loguat leaf 69g, pappy shell 50g, tuber of stemona 15g, Cynanchum glaucescens 9g, root bark of white mulberry 6g, balloonflower root 6g is taken to add water to cook three times, every time 1.5h, collecting decoction, filtration, about 750ml is concentrated into, adds sodium benzoate 2.5g, is stirred to dissolve, adds sucrose 600g, continue to add Heat is kept for 20 minutes to boiling, and is stood, filtration, is added citric acid 0.5g, the essence and menthol 0.15g that are dissolved with ethanol, is stirred Mix, mix, stand, add water to 1000ml, mix, produce；
    Method of quality control：
    A, discrimination method：Intensified loquet distillate 80ml is taken, with the n-butanol shaking extraction 2 times, each 50ml of ammonia solution saturation, is merged N-butanol extracting liquid, washed with the water 50ml of n-butanol saturation, discard water lotion, divided and take n-butanol layer, be evaporated, residue adds ethanol 1ml makes dissolving, as need testing solution；Loguat leaf control medicinal material 2g is taken, adds water 50ml, is heated to reflux 1h, let cool, filtered with cotton Cross, take filtrate, extracted 2 times, each 30ml with the n-butanol shaking of ammonia solution saturation, while n-butanol extracting liquid is merged, Using being made in the same way of loguat leaf control medicinal material solution；Cynanchum glaucescens control medicinal material 1g is taken, is made in the same way of Cynanchum glaucescens control medicinal material solution；According to Thin-layered chromatography draws above-mentioned each 4-8 μ l of three kinds of solution, puts respectively on same silica gel g thin-layer plate, with 5：2 toluene-acetone For solvent, deploy, take out, dry, be placed under 365nm ultraviolet lamp and inspect；In test sample chromatogram, with Cynanchum glaucescens comparison medicine Wood color is composed on corresponding position, shows the fluorescence spot of same color；Spray is heated to 20% phosphomolybdic acid ethanol solution at 105 DEG C Spot development is clear, is inspected under daylight, in test sample chromatogram, on position corresponding with loguat leaf control medicinal material chromatogram, shows phase With the spot of color；Pappy shell control medicinal material 2g is taken, control medicinal material solution is made in the same way of, is tested according to thin-layered chromatography, take confession Test sample solution and each 4-8 μ l of pappy shell medicinal material solution, put respectively on same silica gel g thin-layer plate, with 5：2 toluene-acetone is Solvent, deploy, take out, dry, spray with dilute bismuth potassium iodide test solution；In test sample chromatogram, corresponding with control medicinal material chromatogram On position, show the spot of same color；
    B, content detection：The morphine in intensified loquet distillate is measured according to high performance liquid chromatography, including chromatographic condition with System suitability, the preparation of reference substance solution, the preparation of need testing solution, measure；The chromatographic condition is applicable with system Property experiment be using octyl silane group silica gel as filler, with acetonitrile -0.01mol/L dipotassium hydrogen phosphate solutions -0.005mol/ L heptane sulfonic acid sodium salts are according to 20：40：40 volume ratio is mobile phase, Detection wavelength 220nm, and number of theoretical plate presses morphine peak Calculate >=2000.
  2. 2. the quality of production control method of intensified loquet distillate according to claim 1, it is characterised in that：In the step b The preparation of reference substance solution is to take morphine reference substance is accurately weighed to put in brown measuring bottle, adds 20% methanol solution containing 5% acetic acid The solution that every 1ml contains 24 μ g is made, produces.
  3. 3. the quality of production control method of intensified loquet distillate according to claim 1, it is characterised in that：In the step b The preparation of need testing solution is that precision measures intensified loquet distillate 3ml, is put in 10ml brown measuring bottles, adds 20% first containing 5% acetic acid Alcoholic solution shakes up to scale, is filtered, produced with 0.45 μm of miillpore filter.
  4. 4. the quality of production control method of intensified loquet distillate according to claim 1, it is characterised in that：In the step b Measure is that precision draws reference substance solution and each 10 μ l of need testing solution respectively, injects liquid chromatograph, determines and produce.
  5. 5. the quality of production control method of intensified loquet distillate according to claim 1, it is characterised in that：The intensified loquet Dew per 1ml containing pappy shell in terms of morphine >=15 μ g.
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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109374815B (en) * 2018-11-01 2021-02-02 贵州大隆药业有限责任公司 Quality detection method of medicinal composition for treating cough, asthma and fullness sensation in chest
CN112730724B (en) * 2020-12-29 2022-08-09 江阴天江药业有限公司 Thin-layer identification method for cynanchum glaucescens formula granules
CN113009040B (en) * 2021-03-15 2022-10-04 玉林市食品药品检验检测中心 Detection method of fingerprint of strong loquat syrup and fingerprint thereof
CN114081934B (en) * 2022-01-20 2022-04-12 江西省中医药研究院 Traditional Chinese medicine composition and quality detection method thereof
CN115845006A (en) * 2022-12-05 2023-03-28 哈尔滨市康隆药业有限责任公司 Preparation method of strong loquat syrup

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1739755A (en) * 2005-09-14 2006-03-01 穆滨 strong loquat syrup and preparation method thereof
CN1857652A (en) * 2006-03-16 2006-11-08 江西滕王阁药业有限公司 Preparing process and inspection method for reinforced loquat distillate with refined honey
WO2011103398A1 (en) * 2010-02-18 2011-08-25 Aegis Sciences Corporation Detection and quantitation of pain medications in oral fluid specimens
CN102370921A (en) * 2010-08-26 2012-03-14 江西济民可信集团有限公司 Detection method of strong loquat dew traditional Chinese preparation
CN102462754A (en) * 2010-10-28 2012-05-23 广西壮族自治区花红药业股份有限公司 Detection method for Chinese medicine preparation for cough relief, phlegm elimination and asthma relief
CN102854281A (en) * 2012-08-29 2013-01-02 哈尔滨市康隆药业有限责任公司 Detection method of sugar-free strong loquat syrup
CN104034838A (en) * 2013-03-07 2014-09-10 广西灵峰药业有限公司 Quality detection method of Corsvenor Momordica Fruit cough-relieving syrup

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080206883A1 (en) * 2007-02-26 2008-08-28 Cody Laboratories, Inc. Hplc method for separation and detection of hydromorphone and related opioid pharmacophores
US8067243B2 (en) * 2008-09-03 2011-11-29 Oregon Medical Laboratories Methods and systems for analyzing medication levels in a sample

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1739755A (en) * 2005-09-14 2006-03-01 穆滨 strong loquat syrup and preparation method thereof
CN1857652A (en) * 2006-03-16 2006-11-08 江西滕王阁药业有限公司 Preparing process and inspection method for reinforced loquat distillate with refined honey
WO2011103398A1 (en) * 2010-02-18 2011-08-25 Aegis Sciences Corporation Detection and quantitation of pain medications in oral fluid specimens
CN102370921A (en) * 2010-08-26 2012-03-14 江西济民可信集团有限公司 Detection method of strong loquat dew traditional Chinese preparation
CN102462754A (en) * 2010-10-28 2012-05-23 广西壮族自治区花红药业股份有限公司 Detection method for Chinese medicine preparation for cough relief, phlegm elimination and asthma relief
CN102854281A (en) * 2012-08-29 2013-01-02 哈尔滨市康隆药业有限责任公司 Detection method of sugar-free strong loquat syrup
CN104034838A (en) * 2013-03-07 2014-09-10 广西灵峰药业有限公司 Quality detection method of Corsvenor Momordica Fruit cough-relieving syrup

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HPLC法测定强力枇杷露(煎膏剂) 中吗啡的含量;周建衡 等;《中医药导报》;20080930;第14卷(第9期);第71-72页 *
蜜炼强力枇杷膏质量标准研究;刘文志 等;《中药材》;20120131;第35卷(第1期);第144-147页 *
高效液相色谱法测定罂黄散中吗啡的含量;马维平 等;《武警医学》;20120630;第23卷(第6期);第480-482页 *

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