TWM616018U - Detecting device - Google Patents

Abstract

A detecting equipment which includes a sample plate, a cell-culture unit, a filter, and a slidable cover. The cell-culture unit is disposed on the sample plate. The cell-culture unit has a first area and a second area therein which are in fluid communication with each other. The filter is disposed in the second area of the cell-culture unit. The slidable cover is slidably disposed on the sample plate and covers the cell-culture unit so as to switchable between a first position and a second position. The slidable cover has an injection hole and an air hole. When the slidable cover is in the first position, the injection hole corresponds to the first area of the cell-culture unit, and when the slidable cover is in the second position, the air hole corresponds to the filter within the second area.

Description

Testing Equipment

This creation is about a detection device, especially a detection device for cell culture.

In order to indirectly detect the influence of external factors on individual cells, "cell culture technology", which cultures cells in an artificially controlled environment, plays a very important role in modern biology. In the implementation, the cell samples collected from the living body will be transferred to a suitable vessel for culture. Moreover, in order to help cells grow quickly and interact, it is necessary to disturb the cell sample.

To this end, a general practice is to insert a pipette into the culture tank of the culture tray to repeatedly inhale and blow the cell sample in it, so as to disturb the cell sample. However, during the process, the cell sample easily splashes and adheres to the pipette, thereby contaminating the pipette. If it is not cleaned, the blowing pipe will take the attached cell samples to different culture tanks, which will affect the culture environment of other cell samples.

According to an embodiment of the present invention, a detection device is disclosed, which includes a sample plate, a culture unit, a filter, and a sliding cover. The culture unit is arranged on the sample tray, and the culture unit has a first accommodating area and a second accommodating area that are connected to each other. The filter is accommodated in the second accommodating area of the culture unit. The sliding cover is slidably arranged on the sample tray and covers the culture unit to switch between a first position and a second position. The sliding cover has an injection hole and a blowing hole. When the sliding cover is in the first position, the injection hole corresponds to the first accommodating area of the culture unit. When the sliding cover is in the second position, the blowing hole corresponds to the filter element in the second accommodating area.

Applying the detection device of this creative embodiment, since the second accommodating area of the culture unit is provided with a filter element, the filter element corresponds to the blowing hole of the sliding cover when the sliding cover is switched, so that the filter element can be blown and sucked. When the air flow disturbs the cell sample, the cell sample is prevented from splashing up to the suction nozzle of the suction device, thereby preventing the cell sample from contaminating the suction nozzle during the disturbance.

The above description of the disclosure of this creation and the description of the following implementation manners are used to demonstrate and explain the spirit and principle of this creation, and to provide a further explanation of the scope of patent application for this creation.

Please refer to Figures 1 to 4. Figure 1 is a three-dimensional schematic diagram showing the detection device of an embodiment of this creation, Figure 2 is an exploded schematic diagram showing the detection device of this creation embodiment, and Figure 3 is a diagram showing the detection device of this creation embodiment Fig. 4 is a schematic cross-sectional view of the culture unit of this creative embodiment.

An embodiment of the present invention proposes a detection device 1 that can be used for cell culture and detection. As shown in the figure, the detection device 1 may include a single plate 20, a plurality of culture units 30a, a plurality of filters F, and a滑盖40。 Slide 40. In addition, the detection device 1 can also be equipped with a blowing device 50 to perform non-contact perturbation on the cultured cell sample (the cell sample 9 shown in FIG. 6 or FIG. 8).

The sample tray 20 can be, but is not limited to, an integrally formed single structure. The sample tray 20 may be in the shape of a box with the top open to the outside and has an accommodating space S, and the accommodating space S can be used for accommodating the culture unit 30a. The culturing unit 30a can be used for holding and culturing cell samples. It should be noted that the arrangement and number of the cultivation units 30a in the accommodating space S as shown in the figure are only for the purpose of facilitating the description, and can be adjusted according to actual needs and are not used to limit the creation.

In addition, in order to facilitate the observation of the culture unit 30a in the accommodating space S from the outside of the sample tray 20, the sample tray 20 and the culture unit 30a can be made of any suitable light-permeable material. In addition, in order to facilitate the positioning of the culture unit 30a provided on the sample tray 20, in this embodiment, the sample tray 20 may further include a plurality of alignment grooves 21 located in the accommodating space S, and the alignment grooves 21 are self-contained The bottom surface of the space S is recessed downward and has substantially the same contour as the culture unit 30a, so that at least part of the culture unit 30a can be accommodated in the alignment groove 21, which helps users to quickly place the culture unit 30a. 30a is removably placed at a specific position in the accommodating space S. It should be noted that the arrangement, number and shape of the alignment slots of the sample plate of this creation can be adjusted according to actual needs, and are not used to limit this creation.

The culture unit 30a can be, but is not limited to, an integrally formed single structure. In this embodiment, the culture unit 30a may include a shell part 310a and a partition wall part 320a. The shell part 310a may be a hollow structure with one end open to the outside, and the partition wall part 320a may be a hollow cylindrical shape with one end open to the outside. Structure, the partition wall part 320a is located in the shell part 310a and the interior of the shell part 310a can be divided into a first accommodating area A1 and a second accommodating area A2, wherein the second accommodating area A2 is the partition wall part 320a The outer portion of the housing portion 310a except for the second housing area A2 belongs to the first housing area A1. In this configuration, the housing portion 310a may be formed with a connecting area connected to the first housing area A1. The injection port 311a, and the partition wall portion 320a may be formed with a blowing and suction port 321a communicating with the second accommodating area A2. In addition, the partition wall portion 320a may further have a plurality of drainage holes 322a, and the drainage holes 322a penetrate the partition wall portion 320a to connect the first accommodating area A1 and the second accommodating area A2. It should be noted that, as long as the first accommodating area A1 and the second accommodating area A2 can reach a certain degree of connectivity, the number and arrangement of the drainage holes can be adjusted according to actual needs, rather than limiting the creation.

The filter F is accommodated in the culture unit 30a. Specifically, the filter element F is accommodated in the partition wall 320a of the culture unit 30a and is located in the second accommodating area A2. The filter element F is a porous structure with micro air-permeable pores, which can allow air flow but has a waterproof effect. The filter F can be made of any suitable material (such as PE polyethylene), as long as it can achieve the required air permeability and waterproof purpose. In addition, in order to stabilize the position of the filter element F, in the present embodiment, the partition wall portion 320a may also have an inner convex pattern 323 formed on the inner surface of the partition wall portion 320a to clamp the filter element F, thereby strengthening the filter element Fixed effect of F. In addition, the number of filter elements is not used to limit the creation, but can be adjusted according to the number of culture units used in combination, for example.

The sliding cover 40 is slidably disposed on the sample tray 20 and covers the top of the culture unit 30a. In this embodiment, the sliding cover 40 is slidable to switch to a first position (as shown in FIGS. 5 and 6). (Shown) and a second position (shown in FIGS. 7 and 8), thereby exposing different parts of the culture unit 30a. Specifically, in this embodiment, the sliding cover 40 may have a plurality of injection holes 41 and a plurality of blowing holes 42. The injection holes 41 and the blowing holes 42 penetrate the sliding cover 40 and communicate with the accommodating space of the sample tray 20. S, and the injection holes 41 and the blowing holes 42 are arranged in groups of two or two, or arranged in a staggered manner, so as to be able to communicate with the injection ports 311a of the culture unit 30a when the sliding cover 40 switches between different positions. With blowing and sucking mouth 321a. It is understandable that the arrangement and number of the injection holes and the blowing holes of the slide cover are not used to limit the creation, but can be adjusted according to the number and arrangement of the culture units that the sample tray is intended to carry.

Here, please refer to Figures 5 to 8. Figure 5 shows a partial enlarged schematic view of the detection device of this creative embodiment when its sliding cover is in the first position, and Figure 6 shows the detection device of this creative embodiment on its sliding cover A partial enlarged schematic side sectional view at the first position. Figure 7 shows a partial enlarged schematic diagram of the detection device of this creative embodiment when its slide cover is switched to the second position. Figure 8 shows the detection device of this creative embodiment in the second position. The partial enlarged schematic side sectional view of the sliding cover when the sliding cover is switched to the second position.

First, as shown in FIGS. 5-6, the sliding cover 40 is in the first position. At this time, the injection hole 41 of the sliding cover 40 is aligned with the injection port 311a of the culture unit 30a so that the injection port 311a is exposed to the outside, but the culture unit 30a At least part or all of the blowing port 321a can still be covered by the sliding cover 40 without being exposed to the outside, whereby the user can inject the cell sample 9 to be cultured into the culture unit 30a from the injection hole 41 of the sliding cover 40 The injection port 311a allows the cell sample 9 to enter the first accommodating area A1 of the culture unit 30a from the injection port 311a, and flow into the second accommodating area A2 surrounded by the partition wall 320a through the drainage hole 322a. Since the drainage hole 322a connects the first accommodating area A1 and the second accommodating area A2, the cell sample 9 can be evenly distributed in the first accommodating area A1 and the second accommodating area A2.

Then, as shown in FIGS. 7-8, the sliding cover 40 can be slid in the direction shown by the arrow to switch to the second position. At this time, the blowing hole 42 of the sliding cover 40 is aligned with the blowing and sucking port 321a of the culture unit 30a. The blowing port 321a is exposed to the outside, and the blowing hole 42 corresponds to the filter F in the second accommodating area A2, whereby one of the blowing nozzles 51 of the blowing device 50 can be docked or inserted into the blowing and sucking of the sliding cover 40 The hole 42 is aligned with the blowing and suction opening 321a of the culture unit 30a to perform repeated suction and blowing operations on the second accommodating area A2 of the culture unit 30a. The airflow generated by the second accommodating area A2 of 30a can penetrate the filter F, thereby disturbing the cell sample 9 at the bottom of the culture unit 30a, so as to help the cell sample 9 to grow rapidly and interact with each other. However, at the same time, due to filtration The member F has a waterproof effect, so even if the cell sample 9 splashes upwards due to airflow disturbance, the filter member F can ensure that the cell sample 9 will not touch or attach to the suction nozzle 51 of the suction device 50, that is, the filter member F can prevent the cell sample 9 from contaminating the blowing nozzle 51 during the process of disturbing the cell sample 9.

In addition, in order to monitor the process of culturing the cell sample 9, as shown in FIGS. 1 and 2, in this embodiment, the detection device 1 may further include a base 60, a circuit board P, and a plurality of image capturing units C. The base 60 can be disposed under the sample tray 20, the circuit board P can be disposed on the base 60 and under the sample tray 20, and the image capturing unit C can be disposed on the circuit board P. The arrangement of the image capturing units C is the same as The number can correspond to the culture unit 30a on the sample tray 20, so as to monitor, record, observe and analyze the culture process of the cell sample 9 in the culture unit 30a in real time.

However, the arrangement and number of image capturing units are not used to limit this creation. For example, it can be adjusted according to the number and arrangement of culture units that the sample tray is scheduled to carry, or it can be adjusted as a single according to other requirements. For example, please refer to FIG. 9. FIG. 9 is an exploded schematic diagram of a detection device according to another embodiment of the present creation. 'Can include a mobile module 80, the mobile module 80 can be set on the base 60, the image capture unit C can be set on the mobile module 80, through the mobile module 80 to perform a biaxial plane under the sample tray 20 Movement to achieve the purpose of a single image capturing unit C correspondingly monitoring multiple cultivation units 30a.

In addition, the cultivating unit 30a of the above-mentioned embodiment is only an example of this creation, and is not intended to limit this creation. The following will list some of the training units of other embodiments of this creation, but it should be stated first that, for the purpose of brief description, the content of the following embodiments is only explained for the parts that are different from the previous embodiments, and the same is repeated. Part of it can be understood from the corresponding paragraphs that have already been described, so please do not repeat them here.

Please refer to FIGS. 10A to 10B. FIG. 10A is a three-dimensional schematic diagram showing the culture unit of another embodiment of the present creation, and FIG. 10B is a cross-sectional schematic diagram showing the culture unit of FIG. 10A. This embodiment proposes a culture unit 30b, the outer shell portion 310b of which may be slightly convex, and the partition wall portion 320b may be slightly hollow and square column shape.

Please refer to FIG. 11. FIG. 11 is a schematic cross-sectional view of a culture unit according to another embodiment of the present creation. This embodiment proposes a culture unit 30c, which can use the housing portion 310b of the previous embodiment, and the drainage hole 322c of the partition wall portion 320c can be expanded to increase the first accommodating area A1c and the second accommodating area A2c The connection efficiency.

Please refer to FIG. 12, which is a schematic cross-sectional view of a culture unit according to another embodiment of the present creation. This embodiment proposes a culture unit 30d, which can use the housing part 310b of the previous embodiment, and the partition wall part 320d may have only a single drainage hole 322d, and a blade member 70 is pivotally connected to the partition wall part 320d. It is located at the drainage hole 322d, so that when the cell sample is disturbed, the blade member 70 can be driven to rotate at the drainage hole 322d, so as to achieve the effect of enhancing the degree of disturbance of the cell sample.

Please refer to FIGS. 13A to 13B. FIG. 13A shows a three-dimensional schematic diagram of the culture unit of another embodiment of the present creation, and FIG. 13B shows a schematic cross-sectional view of the culture unit of FIG. 13A. This embodiment proposes a culture unit 30e, the housing part 310e of which can be slightly quadrangular, and the partition wall part 320e is hollow cylindrical and has a plurality of first accommodating areas A1e and a second accommodating area A2e. The drainage hole 322e and a plurality of second drainage holes 324e, wherein the distance between the first drainage hole 322e and the second drainage hole 324e to the filter F is different. As shown in the figure, the second drainage hole 324e is larger than the first drainage hole 322e. It is closer to the filter F, and the second drainage hole 324e can be, but is not limited to, a hole with a longer extension length than the first drainage hole 322e, so that the cell sample can pass through the second drainage hole 324e when the water level is too high due to airflow disturbance Flowing back to the first accommodating area A1e not only reduces the amount of splashing of the cell sample 9 to the filter F, but also enhances the overall disturbance effect.

In summary, according to the detection device disclosed in this creative embodiment, since the second accommodating area of the culture unit is provided with a filter, the filter is made to correspond to the blowing hole of the sliding cover when the sliding cover switches positions, thereby , The filter element can prevent the cell sample from splashing up to the suction nozzle of the suction device during the process of disturbing the cell sample by the blowing and suction airflow, thereby preventing the cell sample from contaminating the suction nozzle during the disturbance process.

In addition, the culture units are all detachably installed, so users can selectively simultaneously culture a small amount or a large amount of multiple or single types of cell samples on the same sample tray according to requirements.

In addition, the detection equipment is also provided with an image capture unit to monitor, record, and observe the culture process of the cell sample in real time.

Although this creation is disclosed above with the aforementioned embodiments, it is not intended to limit this creation. Without departing from the spirit and scope of this creation, all changes and modifications made are within the scope of patent protection of this creation. For the scope of protection defined by this creation, please refer to the attached scope of patent application.

1. 1’: Testing equipment 9: Cell sample 20: sample tray 21: Alignment slot 30a, 30b, 30c, 30d, 30e: culture unit 40: Slide cover 41: Injection hole 42: Blow hole 50: Blow and suction device 51: Blow nozzle 60: Base 70: blade parts 80: mobile module 310a, 310b, 310e: shell part 311a: Injection port 320a, 320b, 320c, 320d, 320e: Partition wall 321a: blowing and sucking mouth 322a, 322c, 322d: drainage hole 323: Inner Convex 322e: first drainage hole 324e: second drainage hole A1, A1c, A1e: the first housing area A2, A2c, A2e: second housing area C: Image capture unit F: Filter P: Circuit board S: accommodating space

Fig. 1 is a three-dimensional schematic diagram showing a detection device according to an embodiment of the present invention. Fig. 2 is an exploded schematic diagram showing the detection device of this creative embodiment. Fig. 3 is a partial enlarged and exploded schematic diagram showing the detection device of this creative embodiment. Fig. 4 is a schematic cross-sectional view showing the cultivation unit of this creative embodiment. Fig. 5 is a partial enlarged schematic diagram showing the detection device of this creative embodiment, in which the sliding cover is located at the first position. Fig. 6 is a partial enlarged schematic side sectional view showing the detection device of this creative embodiment, in which the sliding cover is located at the first position. FIG. 7 is a partial enlarged schematic diagram showing the detection device of this creative embodiment, in which the sliding cover is located in the second position. Fig. 8 is a partial enlarged schematic side sectional view showing the detection device of this creative embodiment, in which the sliding cover is located at the second position. Fig. 9 is an exploded schematic diagram showing the detection device of another embodiment of the present creation. Fig. 10A is a three-dimensional schematic diagram showing the cultivation unit of another embodiment of the present creation. Fig. 10B is a schematic cross-sectional view showing the culture unit of Fig. 10A. Fig. 11 is a schematic cross-sectional view showing the cultivation unit of another embodiment of the present creation. Fig. 12 is a schematic cross-sectional view of the cultivation unit of another embodiment of the present creation. Fig. 13A is a three-dimensional schematic diagram showing the cultivation unit of another embodiment of the present creation. Fig. 13B shows a schematic cross-sectional view of the culture unit of Fig. 13A.

1: Testing equipment

20: sample tray

30a: Culture unit

40: Slide cover

50: Blow and suction device

51: Blow nozzle

60: Base

Claims (11)

A testing equipment, including: The same dish; a culture unit, set on the sample tray, wherein the culture unit has a first containing area and a second containing area connected to each other; a filter element is accommodated in the first of the culture unit Two accommodating areas; and a sliding cover slidably disposed on the sample tray and covering the culture unit to switch between a first position and a second position, wherein the sliding cover has an injection hole and a blowing Suction hole; wherein the injection hole corresponds to the first accommodating area of the culture unit when the sliding cover is in the first position, and the blowing hole corresponds to the second accommodating area when the sliding cover is in the second position The filter element in the area. The detection device according to claim 1, wherein the culture unit further includes an injection port and a blowing and suction port, and when the sliding cover is in the first position, the first accommodating area is connected to the sliding cover via the injection port For the injection hole, when the sliding cover is in the second position, the second accommodating area is connected to the blowing hole of the sliding cover via the blowing and suction opening. The detection device according to claim 2, wherein the blowing hole of the sliding cover and the blowing hole of the culture unit have substantially the same size. The detection device according to claim 2, wherein the filter element has a plurality of micro vents, and the filter element and the blowing port of the culture unit have substantially the same size. The detection device according to claim 2, wherein when the sliding cover is in the second position, the sliding cover covers the injection port of the culture unit. The detection device according to claim 1, wherein the culture unit includes a housing part and a partition wall part, and the partition wall part is located in the housing part to divide the housing part into the first accommodating area and the second accommodating area. In the accommodating area, the partition wall surrounds the second accommodating area, and the partition wall includes a drainage hole to make the first accommodating area communicate with the second accommodating area. The detection device according to claim 6, wherein the partition wall includes a plurality of drainage holes, surrounding and communicating with the first accommodating area and the second accommodating area. The detection device according to claim 6, further comprising a blade member pivotally connected to the partition wall and rotatably adjacent to the drainage hole. The detection device according to claim 6, wherein the partition wall includes a first drainage hole and a second drainage hole, and the distance between the first drainage hole and the second drainage hole to the filter element is different. The detection device according to claim 1, wherein the sample tray has an alignment groove, the culture unit is accommodated in the alignment groove, and the alignment groove and the culture unit have substantially the same outline. The detection device according to claim 1, further comprising an image capturing unit located on a side of the sample tray opposite to the culture unit for capturing images of the culture unit, wherein the sample tray is light-transmissive .

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