TWM468519U - Sweet potato protein purification and extraction system - Google Patents

Description

Sweet potato protein purification extraction system

The present invention relates to a purification extraction system, in particular to a purified sweet potato protein purification extraction system.

Sweet potato, also known as sweet potato, sweet potato, sweet potato, sweet potato, etc., is an annual plant of the genus Convolvulaceae, which is a kind of healthy food for both medicine and food. Sweet potato contains dietary fiber, carotene, vitamin A, B, C, E and more than 10 kinds of trace elements such as potassium, iron, copper, selenium and calcium. It has high nutritional value and can be called the most balanced food.

In the past, the sweet potato protein contained in sweet potato was known to have a natural trypsin inhibitor (TI) and a glycoprotein. Sweet potato protein stimulates the proximal small intestine to secrete cholecystokinin (CCK), which binds to the CCK-A receptor (CCKA) in the liver and central nervous system, and transmits the signal of satiety to the hypothalamus of the brain. The human body begins to release CCK after eating. Therefore, sweet potato protein can increase satiety and effectively reduce food intake. In addition, sweet potato protein can reduce blood lipids (triglycerides, cholesterol) or increase leptin secreted by adipocytes by activating peroxisome Proliferators Activated Receptor (PPAR). Reduce appetite and stimulate peripheral tissues to consume calories. Therefore, in conjunction with the release of CCK, weight control is achieved by reducing food intake and calorie consumption.

However, for a long time, the process of extracting sweet potato protein is easy to destroy its biological activity, so that even if it is known that the sweet potato protein contained in the sweet potato extract has multiple beneficial effects on health, it is still difficult to retain sweet potato protein during the extraction process. Biological activity.

In view of the above, an object of the present invention is to provide a sweet potato protein purification and extraction system capable of retaining the biological activity of sweet potato protein, which comprises a pretreatment unit for stirring, pulverizing and homogenizing a sweet potato raw material into a sweet potato powder; An extraction unit, connected to the pretreatment unit, is an extraction solvent added to the sweet potato powder to produce a sweet potato extract, wherein the extraction solvent is composed of acetic acid and sodium chloride, preferably 1% to 5% acetic acid. And 1% to 10% of sodium chloride; a centrifugation unit, connected to the extraction unit, the sweet potato extract is separated by centrifugation to separate a sweet potato liquid and a sweet potato precipitate, wherein the sweet potato liquid contains a protease inhibitor II (Trypsin inhibitor II); a heating unit, connected to the centrifugal unit, the temperature of the sweet potato liquid is warmed to a first temperature range to remove components other than the protease inhibitor II in the sweet potato liquid, and then heated The sweet potato liquid is cooled to room temperature; and at least one filtration concentration unit is connected to the heating unit to remove the macromolecule in the cooled sweet potato liquid to produce a sweet potato protein Taking the protein molecular system of the sweet potato protein extract from 20KD to 200KD; wherein the centrifugation method is a centrifuge speed of 1,500 rpm to 2,500 rpm; the heating unit is a heating tank; the first temperature range is 72 ° C to 80 ° C; and the extraction unit is subjected to extraction at a second temperature range, the second temperature range is 0 ° C to 10 ° C, so the novel sweet potato protein purification extraction system is beneficial to the biological activity of the sweet potato protein extract Extraction.

In an embodiment of the present invention, the pretreatment unit of the sweet potato protein purification and extraction system comprises: a raw material tank for storing the sweet potato raw material; a washing tank, connecting the raw material tank, and washing the sweet potato raw material; And connecting the washing tank to grind the sweet potato raw material into a sweet potato fine particle, wherein the grinding machine is a serrated grinder; and a homogenizer connected to the grinder to pulverize the sweet potato fine particles into sweet potato powder. In a preferred embodiment, the grinder is a serrated grinder.

In an embodiment of the present invention, the filtration concentration unit of the sweet potato protein purification extraction system comprises: a first heavy filtration concentration device and a second heavy filtration concentration device, wherein the first heavy filtration concentration device is a filtration Membrane filtration concentrating device, with 0.5 atmosphere pressure to 2 Removing the larger pore size of the sweet potato liquid under atmospheric pressure; the second heavy filtration concentration device is a microfiltration membrane filtration and concentration device, wherein the microfiltration membrane filtration and concentration device removes the macromolecule in the sweet potato liquid, and The macromolecular system is a molecule having a relatively large molecular weight, and the protein molecule of the finally produced sweet potato protein extract is 20KD to 200KD.

In an embodiment of the present invention, the sweet potato protein purification extraction system further comprises a freeze drying unit connected to the filtration concentration unit to facilitate removal of moisture from the sweet potato protein extract to maintain the sweet potato protein extract organism. active.

The novel sweet potato protein purification and extraction system provides the sweet potato protein extract, which not only retains the activity of the sweet potato protein, but also enables the sweet potato protein extract to regulate blood sugar and blood fat, and has the advantages of accelerating fat decomposition, promoting metabolism and increasing satiety. Efficacy, so that the general public can use the sweet potato protein extract extracted by the novel sweet potato protein purification extraction system to obtain rich nutrients in sweet potato. The sweet potato protein purification and extraction system provided by the novel has the advantages that the conventional traditional extraction system lacks, because the conventional technology cannot extract the sweet potato protein with the active ingredient, so the sweet potato protein extracted by the novel system has good stability and Extend the shelf life of the active ingredient.

1‧‧‧Sweet potato protein purification extraction system

10‧‧‧Pretreatment unit

20‧‧‧Extraction unit

30‧‧‧ Centrifugal unit

40‧‧‧heating unit

50‧‧‧Filter concentration unit

100‧‧‧Material tank

110‧‧‧cleaning trough

120‧‧‧ Grinder

130‧‧‧Homogeneous machine

The first figure is a schematic diagram of a sweet potato protein purification system according to a preferred embodiment of the present invention.

The second figure is a schematic diagram of a pretreatment unit of a sweet potato protein purification system according to a preferred embodiment of the present invention.

The present invention provides a sweet potato protein purification extraction system, which is a complete and clear disclosure of the technical contents and the purpose of the present invention. The following detailed description, together with reference to the first figure, shows a preferred embodiment of the present invention. Schematic diagram of the sweet potato protein purification extraction system, the novel sweet potato protein purification extraction system 1 comprises a pretreatment unit 10 for mixing, pulverizing and homogenizing the sweet potato into a sweet potato powder; an extraction unit 20 , connected to the pretreatment Unit 10 , adding an extraction solvent to the sweet potato powder to produce a sweet potato extract, wherein the extraction solvent is composed of acetic acid and sodium chloride, preferably 1% to 5% acetic acid and 1% to 10%. Sodium chloride; a centrifugation unit 30 , connected to the extraction unit 20 , and separating the sweet potato extract into a sweet potato liquid and a sweet potato sediment by centrifugation at a centrifuge speed of 1,500 rpm to 2,500 rpm, wherein the sweet potato liquid a protease inhibitor II (Trypsin inhibitor II); a heating unit 40 , connected to the centrifugal unit 30, wherein the heating unit is preferably a heating tank, the sweet potato liquid The temperature is heated to a first temperature range to remove components other than the protease inhibitor II in the sweet potato liquid, and then the warmed sweet potato liquid is cooled to room temperature, and the first temperature range is 72 ° C to 80 ° C. And at least one filtration concentration unit 50 , connected to the heating unit 40 , removing the macromolecule in the cooled sweet potato liquid to produce a sweet potato protein extract, the protein molecule of the sweet potato protein extract is 20KD to 200KD; The extraction unit 20 performs extraction at a second temperature range of 0 ° C to 10 ° C. The novel sweet potato protein purification extraction system is beneficial to the biologically active extraction of sweet potato protein extract.

In a preferred embodiment of the present invention, as shown in the second figure, the pretreatment unit 10 of the sweet potato protein purification and extraction system comprises: a raw material tank 100 for storing the sweet potato raw material; a washing tank 110 for connecting the raw material The tank 100 is used to wash the sweet potato raw material; a grinder 120 is connected to the washing tank 110 to grind the sweet potato raw material into sweet potato fine particles, wherein the grinder 120 is preferably a serrated grinder; and a homogenizer 130 The grinder 120 is connected to the sweet potato fine particles to be pulverized into sweet potato powder.

In an embodiment of the present invention, the centrifugation unit 30 of the sweet potato protein purification and extraction system comprises: a feed port, a drain port and a discharge port, wherein the feed port communicates with the extracting unit 20 for The sweet potato extract produced by the extraction unit is introduced into the centrifugation unit 30 , and the sweet potato extract is separated into a sweet potato liquid and a sweet potato sediment by centrifugation at a rotation speed of 1,500 rpm to 2,500 rpm, and the sweet potato liquid is discharged through the liquid discharge port. The sweet potato sediment is discharged through the discharge port, and the liquid discharge port is further connected to the heating unit 40 , and the sweet potato liquid is introduced into the heating unit 40 .

In an embodiment of the present invention, the filtration concentration unit 50 of the sweet potato protein purification extraction system comprises: a first heavy filtration concentration device and a second heavy filtration concentration device, wherein the first heavy filtration concentration device is filtered. The membrane filtration concentrating device removes particles having a larger pore diameter in the sweet potato liquid at a pressure of 0.5 atmosphere to 2 atmosphere; the second heavy filtration concentrating device is a microfiltration membrane filtration concentrating device, wherein the microfiltration membrane filtration concentrating device is The macromolecule in the sweet potato liquid is removed, and the macromolecular system is a molecular weight larger molecule, and the protein molecule of the finally produced sweet potato protein extract is 20KD to 200KD.

In an embodiment of the present invention, the sweet potato protein purification extraction system further comprises a freeze-drying unit connected to the filtration concentration unit, and the sweet potato protein extract is lyophilized to remove moisture of the sweet potato protein extract, thereby forming The sweet potato protein extract is concentrated and dried. The relevant conditions for the freeze-drying are: drying temperature -40 ° C to -60 ° C, vacuum degree 2 to 30 millitorr, freeze-drying for 12 hours, and the sweet potato protein extract is concentrated and dried to maintain the sweet potato. Biological activity of protein extracts.

In summary, the present invention fully utilizes the centrifugation unit of the sweet potato protein purification extraction system to separate the sweet potato extract into a sweet potato liquid and a sweet potato precipitate by centrifugation at a centrifuge speed of 1,500 rpm to 2,500 rpm, and utilize the sweet potato protein. At least one filtration concentration unit of the purification extraction system is subjected to a multiple filtration concentration step to obtain a biologically active sweet potato protein extract. The present invention proposes a sweet potato protein extraction system different from the past, which makes the sweet potato protein extracted by the novel sweet potato protein extraction system have the advantages of good stability and prolonging the shelf life of the active ingredients.

However, the above description is only the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modifications or alterations made by those skilled in the art without departing from the spirit and scope of the present invention are It should be covered by the scope of this new patent.

1‧‧‧Sweet potato protein purification extraction system

10‧‧‧Pretreatment unit

20‧‧‧Extraction unit

30‧‧‧ Centrifugal unit

40‧‧‧heating unit

50‧‧‧Filter concentration unit

Claims (15)

A sweet potato protein purification and extraction system comprises at least: a pretreatment unit, a sweet potato raw material is stirred, pulverized and homogenized into a sweet potato powder; an extraction unit is connected to the pretreatment unit, and an extraction solvent is added to the sweet potato powder. To produce a sweet potato extract; a centrifugation unit, connected to the extraction unit, and centrifuging the sweet potato extract to separate a sweet potato liquid and a sweet potato precipitate, wherein the sweet potato liquid contains a protease inhibitor II (Trypsin inhibitor II) a heating unit, connected to the centrifugal unit, the temperature of the sweet potato liquid is warmed to a first temperature range to remove components other than the protease inhibitor II in the sweet potato liquid, and then the warmed sweet potato liquid is cooled to And at least one filtration concentration unit connected to the heating unit to remove macromolecules in the cooled sweet potato liquid to produce a sweet potato protein extract. The sweet potato protein purification and extraction system according to claim 1, wherein the pretreatment unit comprises: a raw material tank for storing the sweet potato raw material; a washing tank, connecting the raw material tank, and washing the sweet potato raw material; The grinder communicates with the washing tank to grind the sweet potato raw material into sweet potato fine particles, and a homogenizer that communicates with the grinder to pulverize the sweet potato fine particles into sweet potato powder. The sweet potato protein purification extraction system according to claim 2, wherein the grinding machine is a sawtooth grinder. The sweet potato protein purification extraction system according to claim 1, wherein the extraction solvent is composed of acetic acid and sodium chloride. The sweet potato protein purification and extraction system according to claim 4, wherein the acetic acid and The sodium chloride content is 1% to 5% acetic acid and 1% to 10% sodium chloride. The sweet potato protein purification extraction system according to claim 1, wherein the centrifugation method is a centrifuge rotation speed of 1,500 rpm to 2,500 rpm. The sweet potato protein purification extraction system according to claim 1, wherein the heating unit is a heating tank. The sweet potato protein purification extraction system of claim 1, wherein the first temperature range is from 72 ° C to 80 ° C. The sweet potato protein purification extraction system according to claim 1, wherein the filtration concentration unit comprises a first heavy filtration concentration device and a second heavy filtration concentration device. The sweet potato protein purification extraction system according to claim 9, wherein the first heavy filtration concentration device is a filtration membrane filtration concentration device. The sweet potato protein purification and extraction system according to claim 10, wherein the filtration membrane filtration and concentration device removes particles having a larger pore diameter in the sweet potato liquid at a pressure of 0.5 atmosphere to 2 atmospheres. The sweet potato protein purification and extraction system according to claim 9, wherein the second heavy filtration concentration device is a microfiltration membrane filtration and concentration device. The sweet potato protein purification extraction system according to claim 12, wherein the microfiltration membrane filtration concentration device removes macromolecules from the sweet potato liquid. The sweet potato protein purification extraction system according to claim 1, wherein the sweet potato protein extract has a protein molecular weight of 20 KD to 200 KD. The sweet potato protein purification extraction system according to claim 1, further comprising a freeze-drying unit connected to the filtration concentration unit to remove water in the sweet potato protein extract to maintain biological activity of the sweet potato protein extract.