CN104509631A - All-coffee-peptide nutrition and preparation method thereof - Google Patents
All-coffee-peptide nutrition and preparation method thereof Download PDFInfo
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- CN104509631A CN104509631A CN201410848904.XA CN201410848904A CN104509631A CN 104509631 A CN104509631 A CN 104509631A CN 201410848904 A CN201410848904 A CN 201410848904A CN 104509631 A CN104509631 A CN 104509631A
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Abstract
The invention discloses an all-coffee-peptide nutrition prepared from coffee beans as main materials and soybeans as auxiliary materials, and a preparation method of the nutrition. The nutrition contains 1-60% by weight of coffee peptides absorbable by a human body and further contains linoleic acid, caffeine, bifidus factor, saponin, VB, Ca, Fe, P, Se, glucose and the like. The preparation method comprises the following steps: roasting the coffee beans at 80-150 DEG C, crushing, adding water, and grinding to prepare pulp; soaking the soybeans, adding water, and grinding to prepare pulp; mixing the coffee pulp with the soybean pulp, homogenizing under the high pressure of 40-60 MPa for 2-4 times, performing grinding and heating at 135-160 DEG C under the pressure of 7-8 kg, adding lipase and cellulase, standing for biochemical treatment, and adding bromelain for constant-temperature biochemical treatment; performing enzyme deactivation and sterilization on the pulp subjected to the biochemical treatment, separating through a plate frame separation system with a food-grade diatomite filter aid to obtain all-coffee-peptide raw liquid, and preparing the all-coffee-peptide raw liquid into the all-coffee-peptide nutrition.
Description
Technical field
The present invention is a kind of biotechnology and modern physics nanometer technology principle used take coffee bean as primary raw material full coffee peptide nutrient food being aided with Soybean production and preparation method thereof.
Background technology
The nutritional labeling that coffee contains is very abundant: moisture content 2.2 grams, 12.6 grams, protein, 16 grams, fat, carbohydrate 46.7 grams, cellulose 9 grams, ash content 4.2 grams, calcium 120 milligrams, 170 milligrams, phosphorus, iron 42 milligrams, 3 milligrams, sodium, vitamin B2 0.12 gram, 3.5 milligrams, nicotinic acid, caffeine 1.3 grams, tannin 8 grams in every 100 grams of coffee beans.And every 100 grams of coffee extract fluid moisture contents 99.5 grams, 0.2 gram, protein, fat 0.1 gram, ash content 0.1 gram, carbohydrate trace, calcium 3 milligrams, 4 milligrams, phosphorus, 2 milligrams, sodium, vitamin B2 0.01 milligram, 0.3 milligram, nicotinic acid.10 grams of coffees are dissolved in hot water, content of caffeine is 0.04 gram, tannin content is 0.06 gram.Caffeine behaves most in all compositions of coffee to gaze at, and it can accelerate the metabolism of human body, makes people keep clearheaded sensitive with thinking; Fat: the fat that coffee includes, local flavor accounts for very important role, and volatile fat is coffee aroma main source; Due to the not easily stripping of protein in coffee, can not be absorbed by the body utilization, and people can only have a taste of its delicious food and stimulation and even refresh oneself when drinking coffee pastime, and the complete nutrients matter of coffee can not be absorbed.
Summary of the invention
It is that primary raw material is aided with a small amount of soybean again through overbaking or after overbaking with coffee bean that problem to be solved by this invention is to provide a kind of, full coffee peptide nutrient food using the nanometer principle in enzyme engineering, histone engineering and modern physics in biotechnology to prepare and preparation method thereof, can turn to the nutritional labelings such as the peptide that people's physical efficiency directly absorbs by coffee protein transduction.Described peptide is the mixture of the compounds such as oligopeptide, small peptide and polypeptide.
Need for achieving the above object to solve following difficult scientific problems: one is solve protein in coffee to be difficult to the problem of stripping; Two is solve coffee the protein not easily digested disconnected and stubborn difficult problem of protein chain; Three is solve usual protein degradation to be easily converted into a large amount of amino acid and the difficult problem be converted under the low conversion rate of peptide that is condensed into a small amount of peptide; Four is solve the problem that coffee for drinking easily causes the three large side effects such as painstaking effort brain pipe disease, induced osteoporosis and carcinogenic danger.
Full coffee peptide nutrient food provided by the invention contains the peptide of 1 ~ 60% percentage by weight, also containing linoleic acid, leukotrienes, oleic acid, caffeine, Oilgosaccharkdes, saponin, Flavonoid substances, phosphatide, VB, calcium, iron, phosphorus, selenium and glucose, also containing a certain amount of water; Described full coffee peptide nutrient food is made by the following method: the ratio of weight and number of coffee bean and soybean is 100 ~ 60: 0 ~ 40; First toasted 30 ~ 50 minutes at 80 ~ 150 DEG C by coffee bean, add water after fragmentation grinding slurrying, and fineness reaches 260 ~ 500 orders; Again by the grinding slurrying that adds water after soybeans soaking, fineness reaches 200 ~ 300 orders; Coffee slurries are mixed latter high-pressure homogeneous 2 ~ 4 times with large soybean milk liquid, homogenization pressure is 40 ~ 60Mpa; By the grinding segmentation at the temperature of 135 ~ 160 DEG C and 7 ~ 8kg pressure of the slurries after homogeneous, and heat 35 ~ 40 minutes; Slurries are cooled to 30 ~ 55 DEG C, add lipase and cellulase and leave standstill biochemical 1.5 ~ 2.5 hours, then temperature is adjusted to 45 ~ 50 DEG C, add bromelain constant temperature biochemistry 4 ~ 8 hours again; Slurries after biochemistry being warming up to 80 ~ 100 DEG C of enzyme sterilizings of going out, is finally under the condition of 68 ~ 85 DEG C in temperature, separating out, obtaining full coffee peptide stoste, and then making full coffee peptide nutrient food by separating out system containing the sheet frame of food grade kieselguhr filter aid.
Full coffee peptide nutrient food of the present invention can be: full coffee Gly-His-Lys, full coffee peptide capsule, full coffee peptide tablet, full coffee peptide oral liquid or full coffee height peptide active motor drink.Can up to 60% for complete its peptide content of coffee Gly-His-Lys, and the peptide content of full coffee peptide oral liquid or active motor beverage is more than 4%.
Its step of preparation method of full coffee peptide nutrient food provided by the invention is as follows:
1) prepare burden: select fresh, free from admixture, without the coffee bean gone mouldy and soybean, the ratio of weight and number of coffee bean and soybean is 100 ~ 60: 0 ~ 40;
2) coffee slurries are prepared
2.1) first coffee bean is toasted 30 ~ 50 minutes at 80 ~ 150 DEG C, be dark brown and aromatic flavour to color, make its albuminous degeneration;
2.2) coffee bean after baking is broken, then add water grinding 3 ~ 5 times, and fineness reaches 260 ~ 500 orders, obtains coffee slurries, makes protein denaturation, more easily degraded and condensation;
3) large soybean milk liquid is prepared
3.1) soak: be soak 8 ~ 10 hours in the water of 20 ~ 35 DEG C in water temperature by soybean;
3.2) grinding segmentation: soaked soybean use water cleans up (usually cleaning 3 ~ 5 times), add water defibrination, large soybean milk liquid is obtained after screenings is separated, again large soybean milk liquid is at least ground 2 times in colloid mill, fineness reaches 200 ~ 300 orders, make protein denaturation, more easily degraded and condensation;
4) mix: stir after being mixed with large soybean milk liquid by coffee slurries, mixing speed is 2700 ~ 3000 revs/min;
5) homogeneous: by mixed serum homogeneous 2 ~ 4 times in high pressure homogenizer, homogenization pressure is 40 ~ 60Mpa, the protein dispersibility of the two is blended evenly, overcome while being conducive to biochemical degradation and fall coffee and easily cause the three large side effects such as painstaking effort brain pipe disease, induced osteoporosis and carcinogenic danger;
6) de-raw meat goes evil: by the grinding segmentation at the temperature of 135 ~ 160 DEG C and 7 ~ 8kg pressure of the slurries after homogeneous, and heat 35 ~ 40 minutes, fineness is 300-350 orders, harmful substance in coffee and large soybean milk liquid is made to be able to passivation, thoroughly overcome and fall coffee and easily bring out cardio-cerebrovascular diseases and the intrinsic anti-trophism of soybean, cause Thyroid Gland Swell, digestive discomfort is felt sick, five large side effects such as flatulence and the easy gout of beans raw meat bitter taste and make coffee and the further sex change of soybean protein;
7) biochemistry: above-mentioned slurries are cooled to 30 ~ 55 DEG C, adds lipase and cellulase stirs, and leaves standstill biochemical 1.5 ~ 2.5 hours, then temperature is adjusted to 45 ~ 50 DEG C, add bromelain to stir, enzyme preparation is fully mixed with slurries, then constant temperature biochemistry 4 ~ 8 hours;
8) to go out enzyme, sterilizing: the slurries after biochemistry are warming up to 80 ~ 100 DEG C, go out 15 ~ 30 seconds enzyme time;
9) separate out: be under the condition of 68 ~ 85 DEG C in temperature by the material after the enzyme that goes out, sterilizing, separating out by separating out system containing the sheet frame of food grade kieselguhr filter aid, obtaining full coffee peptide stoste, and then making full coffee peptide nutrient food.
The peptide amount that contains of above-mentioned full coffee peptide stoste is 0.8 ~ 1.5% (percentage by weight) left and right, can do multistage concentrated according to the actual requirements through multistage concentration systems, peptide content can be made to reach more than 4%, then through multistage filtering process, by vacuum spray drying system, full coffee Gly-His-Lys can be drawn or make full coffee peptide capsule or tablet again, or making full coffee peptide oral liquid or full coffee height peptide active motor beverage by aseptic subpackaged.
Above-mentioned steps 1) in the ratio of weight and number of coffee bean and soybean be 100 ~ 60: 0.5 ~ 40;
Above-mentioned steps 2.2) in the ratio of weight and number of coffee bean and water be preferably 1: 6 ~ 8; Step 3.2) in the ratio of weight and number of soybean (dry soybean) and water be preferably 1: 6 ~ 8.
Step 7) preferred version be: above-mentioned slurries are cooled to 30 ~ 35 DEG C, add and account for the lipase of slurry weight 0.03 ~ 0.05% and the cellulase of 0.01 ~ 0.025% stirs, leave standstill biochemical 1.5 ~ 2 hours, then temperature is adjusted to 45 ~ 50 DEG C, add the bromelain accounting for slurry weight 0.1 ~ 0.5% to stir, enzyme preparation is fully mixed with slurries, then constant temperature biochemistry 6 ~ 8 hours.
Step 9) in separate out the preferred plan of effect and be: the weight containing the food grade kieselguhr filter aid be coated on sheet frame in the sheet frame precipitation system of food grade kieselguhr filter aid is calculated as 0.5 ~ 0.8 kilogram/square metre by sheet frame screen area.
The present invention adopts biotechnology to pass through unique technique and the enforcement of crucial actual parameter, not only thoroughly overcome the 3 large side effects that coffee is intrinsic, remain the original trophic function of coffee, have also obtained and be rich in as human body is direct, the peptide absorbed completely, and can not to human liver, kidney, spleen, the organs such as brain cause hyperosmosis, have and directly strengthen human life vigor and immunologic function and non-oxidizability, scavenging free radicals, norcholesterol, anallergic promotes lipid metaboli, reducing blood lipid, hypotensive, anti-inflammatory, beauty treatment, antifatigue, anti-ageing, anticancer, suppress harmful bacteria infringement and promote the special dietary health cares such as growth of probiotics breeding, solve this difficult problem that coffee albumen for a long time is not easily absorbed by the body, coffee protein once-used degradeable is made to be condensed into the conversion ratio of peptide up to more than 80%.
Detailed description of the invention
Below in conjunction with specific embodiment, content of the present invention is described in detail.
1) choose 70kg without going mouldy, free from admixture, fresh coffee bean and 30kg soybean;
2) coffee bean of 70kg is put the oven for baking 50 minutes that temperature is 80 DEG C;
3) by coffee bean crusher in crushing, then add pure water 280kg, carry out grinding 3 times, fineness reaches 260 orders, obtains coffee slurries;
4) 30kg soybean is placed in water temperature be 20 DEG C water soak 8 hours;
5) soaked soybean pure water is cleaned 3 times, then add pure water 180kg and carry out screenings separating treatment, the slurries after separation grind 2 times in colloid mill, and fineness reaches 200 orders, obtain large soybean milk liquid;
6) coffee slurries and large soybean milk liquid are mixed, fully stir in the agitator tank of 2700 revs/min, make the two mix;
7) above-mentioned mixed serum to be entered in high pressure homogenizer homogeneous 2 times, homogenization pressure is 400kg;
8) by the process 35 minutes at the temperature of 135 DEG C and 7 atmospheric pressures of the slurries after homogeneous, fineness of grind is 300 orders;
9) above-mentioned slurries are done 10 second time by pipe heat exchanger and be cooled to 30 DEG C; Add in slurries after the cooling period and account for the lipase of slurry weight 0.03% and the cellulase of 0.01% stirs, leave standstill biochemical 1.5 hours, then temperature is adjusted to 45 DEG C, add the bromelain of 0.1%, through high-speed stirred, enzyme preparation is fully mixed with slurries, keeps constant temperature biochemical 8 hours;
10) material is warming up to 100 DEG C of enzymes that go out, sterilizing through pipe heat exchanger by the slurries after biochemistry, goes out 15 seconds enzyme time;
11) cross the diatomaceous sheet frame of food-grade by precoating to separate out system and separate out full coffee peptide about stoste 450kg (containing peptide amount 0.8 ~ 1.5%), wherein the precoating amount of food grade kieselguhr filter aid presses the calculation of sheet frame screen area is 0.5%kg/ square metre, sheet frame filter screen fineness is 600 ~ 3000 orders, and the sheet frame filter screen fineness of this example is 1000 orders;
12) the peptide liquid of above-mentioned precipitation is full coffee peptide stoste, carry out multistage full coffee peptide dietary liquid about the 200kg of concentrated more than 4% according to need, now, aseptic subpackagedly then after multistage filtering and sterilizing full coffee peptide oral liquid or full coffee height peptide active motor drink is made; Or obtain 20kg full coffee Gly-His-Lys by vacuum spray drying system or make full coffee peptide capsule or tablet again.
Claims (7)
1. a full coffee peptide nutrient food, it is characterized in that the coffee peptide containing 1 ~ 60% weight ratio, also containing linoleic acid, leukotrienes, oleic acid, caffeine, Oilgosaccharkdes, saponin, Flavonoid substances, phosphatide, VB, calcium, iron, phosphorus, selenium and glucose, also containing a certain amount of water; Described full coffee peptide nutrient food is made by the following method: the ratio of weight and number of coffee bean and soybean is 100 ~ 60: 0 ~ 40; First toasted 30 ~ 50 minutes at 80 ~ 150 DEG C by coffee bean, add water after fragmentation grinding slurrying, and fineness reaches 260 ~ 500 orders; Again by the grinding slurrying that adds water after soybeans soaking, fineness reaches 200 ~ 300 orders; Coffee slurries are mixed latter high-pressure homogeneous 2 ~ 4 times with large soybean milk liquid, homogenization pressure is 40 ~ 60Mpa; By the grinding segmentation at the temperature of 135 ~ 160 DEG C and 7 ~ 8kg pressure of the slurries after homogeneous, and heat 35 ~ 40 minutes; Slurries are cooled to 30 ~ 55 DEG C, add lipase and cellulase and leave standstill biochemical 1.5 ~ 2.5 hours, then temperature is adjusted to 45 ~ 50 DEG C, add bromelain constant temperature biochemistry 4 ~ 8 hours again; Slurries after biochemistry being warming up to 80 ~ 100 DEG C of enzyme sterilizings of going out, is finally under the condition of 68 ~ 85 DEG C in temperature, separating out, obtaining full coffee peptide stoste, and then making full coffee peptide nutrient food by separating out system containing the sheet frame of food grade kieselguhr filter aid.
2. full coffee peptide nutrient food according to claim 1, is characterized in that full coffee peptide nutrient food is: full coffee Gly-His-Lys, full coffee peptide capsule, full coffee peptide tablet, full coffee peptide oral liquid or full coffee height peptide active motor beverage.
3. the preparation method of full coffee peptide nutrient food described in claim 1, is characterized in that step is as follows:
1) prepare burden: the ratio of weight and number of coffee bean and soybean is 100 ~ 60: 0 ~ 40;
2) coffee slurries are prepared
2.1) first coffee bean is toasted 30 ~ 50 minutes at 80 ~ 150 DEG C;
2.2) coffee bean after baking is broken, then add water grinding 3 ~ 5 times, and fineness reaches 260 ~ 500 orders, obtains coffee slurries;
3) large soybean milk liquid is prepared
3.1) soak: be soak 8 ~ 10 hours in the water of 20 ~ 35 DEG C in water temperature by soybean;
3.2) grinding segmentation: soaked soybean use water cleans up, and add water defibrination, obtain large soybean milk liquid after screenings is separated, larger soybean milk liquid is at least ground 2 times in colloid mill, fineness reaches 200 ~ 300 orders;
4) mix: stir after being mixed with large soybean milk liquid by coffee slurries, mixing speed is 2700 ~ 3000 revs/min;
5) homogeneous: by mixed serum homogeneous 2 ~ 4 times in high pressure homogenizer, homogenization pressure is 40 ~ 60Mpa;
6) de-raw meat goes evil: by the grinding segmentation at the temperature of 135 ~ 160 DEG C and 7 ~ 8kg pressure of the slurries after homogeneous, and heat 35 ~ 40 minutes, fineness reaches 300 ~ 350 orders;
7) biochemistry: above-mentioned slurries are cooled to 30 ~ 55 DEG C, adds lipase and cellulase stirs, and leaves standstill biochemical 1.5 ~ 2.5 hours, then temperature is adjusted to 45 ~ 50 DEG C, add bromelain to stir, enzyme preparation is fully mixed with slurries, then constant temperature biochemistry 4 ~ 8 hours;
8) to go out enzyme, sterilizing: the slurries after biochemistry are warming up to 80 ~ 100 DEG C, go out 15 ~ 30 seconds enzyme time; 9) separate out: be under the condition of 68 ~ 85 DEG C in temperature by the material after the enzyme that goes out, sterilizing, separating out by separating out system containing the sheet frame of food grade kieselguhr filter aid, obtaining full coffee peptide stoste, and then making full coffee peptide nutrient food.
4. preparation method according to claim 3, is characterized in that: step 1) in the ratio of weight and number of coffee bean and soybean be 100 ~ 60: 0.5 ~ 40.
5. the preparation method according to claim 3 or 4, is characterized in that:
Step 2.2) in the ratio of weight and number of coffee bean and water be 1: 6 ~ 8;
Step 3.2) in the ratio of weight and number of soybean and water be 1: 6 ~ 8.
6. the preparation method according to claim 3 or 4, is characterized in that:
Step 7) above-mentioned slurries are cooled to 30 ~ 35 DEG C, add and account for the lipase of slurry weight 0.03 ~ 0.05% and the cellulase of 0.01 ~ 0.025% stirs, leave standstill biochemical 1.5 ~ 2 hours, then temperature is adjusted to 45 ~ 50 DEG C, add the bromelain accounting for slurry weight 0.1 ~ 0.5% to stir, enzyme preparation is fully mixed with slurries, then constant temperature biochemistry 6 ~ 8 hours.
7. the preparation method according to claim 3 or 4, is characterized in that:
Step 9) in be calculated as 0.5 ~ 0.8 kilogram/square metre containing the weight of food grade kieselguhr filter aid be coated on sheet frame in the sheet frame precipitation system of food grade kieselguhr filter aid by sheet frame screen area.
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Cited By (5)
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| CN105368642A (en) * | 2015-12-15 | 2016-03-02 | 贵州大学 | Production method for preparing coffee compound wine with assistance of enzyme method |
| CN109007191A (en) * | 2018-09-06 | 2018-12-18 | 张家界金驰天问农业科技有限公司 | giant salamander active peptide coffee |
| CN111248327A (en) * | 2020-01-19 | 2020-06-09 | 北京工商大学 | Processing method for improving quality of coffee beans by using soybean extract and coffee beans |
| CN114480536A (en) * | 2021-12-17 | 2022-05-13 | 云南文山坤七药业股份有限公司 | Preparation method and application of soybean, coffee and sealwort bioactive peptide |
| CN114517219A (en) * | 2021-12-17 | 2022-05-20 | 云南文山坤七药业股份有限公司 | Preparation method and application of soybean, coffee and gastrodia elata bioactive peptide |
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