CN114517219A - Preparation method and application of soybean, coffee and gastrodia elata bioactive peptide - Google Patents
Preparation method and application of soybean, coffee and gastrodia elata bioactive peptide Download PDFInfo
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- CN114517219A CN114517219A CN202111550111.6A CN202111550111A CN114517219A CN 114517219 A CN114517219 A CN 114517219A CN 202111550111 A CN202111550111 A CN 202111550111A CN 114517219 A CN114517219 A CN 114517219A
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- gastrodia elata
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- 230000000975 bioactive effect Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
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- URLZCHNOLZSCCA-VABKMULXSA-N Leu-enkephalin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)CNC(=O)CNC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=CC=C1 URLZCHNOLZSCCA-VABKMULXSA-N 0.000 claims description 2
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- 240000002319 Citrus sinensis Species 0.000 description 4
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- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
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- JEKMKNDURXDJAD-HWUKTEKMSA-N kahweol Chemical compound C([C@@H]1C[C@]2(C[C@@]1(CO)O)CC1)C[C@H]2[C@@]2(C)[C@H]1C(C=CO1)=C1C=C2 JEKMKNDURXDJAD-HWUKTEKMSA-N 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
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- 235000010447 xylitol Nutrition 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Engineering & Computer Science (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Nutrition Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Birds (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Seasonings (AREA)
Abstract
A preparation method and application of soybean, coffee and rhizoma gastrodiae bioactive peptides are disclosed, wherein the raw materials comprise soybean, rhizoma gastrodiae and coffee, and the specific method comprises the following steps: mixing 30-50 parts of soybean, 5-15 parts of gastrodia elata and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the gastrodia elata and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, and inactivating enzymes after enzymolysis; blending with purified water according to the proportion of 1-5%, adding flavoring agent, plant preservative and purified water to obtain raw materials, and making into cosmetics, foods or medicines and health products. The gastrodia elata has the effects of improving eyesight and remarkably enhancing memory, has obvious protection and regulation effects on the cerebral nervous system of a human body, can enhance the resolution capability of optic nerves, and simultaneously can play the roles of easing pain, inhibiting excitation and resisting convulsion.
Description
Technical Field
The invention relates to the field of bioactive peptides, in particular to a preparation method and application of a soybean, coffee and gastrodia elata bioactive peptide.
Background
Bioactive Peptides (BAP) are a generic term for various Peptides ranging from dipeptides to complex linear, cyclic structures, composed of 20 natural amino acids in different compositions and arrangements in proteins, and are multifunctional compounds derived from proteins. The bioactive peptide has various human metabolism and physiological regulation functions, is easy to digest and absorb, has the effects of promoting immunity, hormone regulation, antibiosis, antivirus, blood pressure reduction, blood fat reduction and the like, and is a functional factor with the hottest research topic and great development prospect in the international food industry at present.
The existing bioactive peptide types do not take soybean, gastrodia elata and coffee as raw materials, so that the bioactive peptide types are not rich enough, the nutritional ingredients are not diverse enough, and the requirements of consumers cannot be met.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a preparation method and application of a soybean, coffee and gastrodia elata bioactive peptide.
In order to achieve the aim of the invention, the invention adopts the specific scheme that:
a preparation method of soybean coffee rhizoma gastrodiae bioactive peptide comprises the following steps of preparing raw materials of soybean, rhizoma gastrodiae and coffee: mixing 30-50 parts of soybean, 5-15 parts of gastrodia elata and 15-40 parts of coffee in proportion, firstly preparing and separating alkaline protein solution, separating the soybean, the gastrodia elata and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, and inactivating enzymes after enzymolysis. .
Further, the protein raw materials of the soybeans, the gastrodia elata and the coffee are prepared by enzymolysis of pepsin and trypsin.
Further, the protein raw materials of the soybeans, the gastrodia elata and the coffee are prepared by papain or compound enzyme Protamex enzymolysis.
Further, the mass ratio of the soybean to the gastrodia elata to the caffeine and enkephalin is (1.3-3.1): (1.3-2.2): (1.8-4.1).
Further, the mass ratio of the soybean, the gastrodia elata and the coffee composite peptide is (1.3-4.0): 0.42.
further, removing surface impurities from the composite micromolecular ionic peptide liquid, centrifuging to obtain supernatant, and thus obtaining the composite soybean gastrodia elata coffee micromolecular (ionic) peptide.
The application of soybean, coffee and gastrodia elata bioactive peptide is characterized in that 1% -5% of the ratio is blended with purified water, and then a flavoring agent, a plant preservative and purified water are added to obtain a raw material, so that the raw material is prepared into cosmetics, foods or medicines and health-care products.
The invention has the beneficial effects that:
the compound plant micromolecule soybean coffee gastrodia elata peptide liquid in a specific molecular weight range is prepared by a specific process, and the three or four components are compounded in a proper proportion, so that the compound can calm the liver, tonify qi, benefit waist and knees, strengthen bones and muscles with a small using amount, increase peripheral and coronary blood flow and protect the heart. Gastrodia elata has the functions of improving eyesight and obviously enhancing memory. The rhizoma Gastrodiae has effects of protecting and regulating brain nervous system, enhancing optic nerve resolution, improving eyesight, and improving intelligence. Simultaneously, the effects of easing pain, inhibiting excitation and resisting convulsion can be achieved.
Detailed Description
The present invention is further described below by way of specific examples, but the present invention is not limited to only the following examples. Variations, combinations, or substitutions of the invention, which are within the scope of the invention or the spirit, scope of the invention, will be apparent to those of skill in the art and are within the scope of the invention.
A composite health-care process using the health-care function of peptide, the fragrance of coffee and the health-care function of gastrodia tuber includes such steps as preparing the alkaline solution of protein, separating from soybean, coffee and gastrodia tuber by mixing (30-70, 5-15 and 15-40). Adding Alcalase protease into the alkaline solution obtained by separating the soybeans, the coffee and the gastrodia elata, carrying out enzymolysis, inactivating enzyme, and centrifuging to obtain supernatant, namely the composite soybean coffee gastrodia elata peptide solution. Carrying out reflux ultrafiltration on the soybean gastrodia elata kahweol peptide liquid in a greenhouse, and carrying out adsorption deodorization on activated carbon during the ultrafiltration. And performing series connection on 9 ultrafiltration membranes in the ultrafiltration operation to intercept molecular weight of 2500 Da-4500 Da and pressure of 0.2 Mpa-1.2 Mpa, treating for 5-20 min, removing inorganic salt ions with relative molecular mass less than or equal to 2500Da, and collecting and filtering the soybean, coffee and rhizoma gastrodiae compound peptide liquid. And adding flavoring agents such as orange and strawberry liquid, fructo-oligosaccharide, xylitol, fruit sugar and the like in a greenhouse to improve the taste of the finished product of the compound peptide liquid. The oral liquid with health care and treatment functions is produced.
Example 1
The raw materials comprise the following components in parts by weight: 80 parts of compound soybean, tall gastrodia tuber and coffee compound peptide, 2 parts of crystalline fructose and food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
s1, dissolving the composite soybean gastrodia elata coffee peptide in water (the mass ratio of materials to water is 1:10), adjusting the pH of the solution to 8.5 by using 1mol/L NaOH solution, adding alkaline protease for enzymolysis, then adjusting the pH to 7 by using 1mol/L HC1 solution, adding neutral protease for enzymolysis, wherein the addition amount is 2500U/kg, inactivating enzyme after the enzymolysis is finished, centrifuging, taking supernate, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 1000U to obtain filtrate with the molecular weight of less than 1000U, thereby obtaining the soybean gastrodia elata coffee composite oligopeptide concentrated solution.
S2, mixing soybean gastrodia elata coffee oligopeptides, dissolving the soybean gastrodia elata coffee oligopeptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 7 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzymes after the reaction is finished, centrifuging, taking supernate, dialyzing, precooling for 50min at the temperature of-10 ℃ for 50min to obtain a composite peptide concentrated solution, adding crystalline fructose and a food additive, and dissolving and mixing uniformly to obtain the plant peptide oral liquid.
Example 2
The raw materials comprise the following components in parts by weight: 75 parts of soybean gastrodia elata coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
s1, dissolving soybean gastrodia elata coffee peptide in water (the mass ratio of materials to water is 1:10), adjusting the pH of the solution to 8.5 by using 1mol/L NaOH solution, adding alkaline protease for enzymolysis, then adjusting the pH to 7 by using 1mol/L HC1 solution, adding neutral protease for enzymolysis, adding 2500U/kg of the neutral protease for enzymolysis, inactivating enzyme after the enzymolysis is finished, centrifuging, taking supernate, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 1000U to obtain filtrate with the molecular weight of less than 1000U, thereby obtaining the soybean gastrodia elata coffee composite oligomeric liquid.
S2, mixing soybean gastrodia elata coffee oligopeptides, dissolving the soybean gastrodia elata coffee oligopeptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 6 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzymes after the reaction is finished, centrifuging, taking supernate to obtain a compound peptide concentrated solution, adding crystalline fructose and a food additive, and dissolving and mixing uniformly to obtain the plant peptide oral liquid.
Comparative example 1
The raw materials comprise the following components in parts by weight: 75 parts of soybean gastrodia elata coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
adding crystalline fructose and food additive into the soybean, rhizoma Gastrodiae and coffee compound peptide, dissolving, and mixing to obtain plant peptide oral liquid.
Comparative example 2
The raw materials comprise the following components in parts by weight: 75 parts of soybean gastrodia elata coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
mixing and dissolving soybean gastrodia elata coffee peptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 6 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzymes after the reaction is finished, centrifuging, taking supernate, adding crystalline fructose and food additives, and uniformly dissolving and mixing to obtain the plant peptide liquid oral liquid.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (7)
1. A preparation method of soybean, coffee and rhizoma gastrodiae bioactive peptides is characterized in that the raw materials comprise soybean, rhizoma gastrodiae and coffee, and the specific method comprises the following steps: mixing 30-50 parts of soybean, 5-15 parts of gastrodia elata and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the gastrodia elata and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, carrying out enzymolysis, and then carrying out enzyme deactivation.
2. The method for preparing bioactive peptide of soybean, coffee and gastrodia elata as claimed in claim 1, wherein the bioactive peptide is prepared by the following steps: the protein raw materials of the soybean, the gastrodia elata and the coffee are prepared by enzymolysis of pepsin and trypsin.
3. The method for preparing bioactive peptide of soybean, coffee and gastrodia elata as claimed in claim 1, wherein the bioactive peptide is prepared by the following steps: the protein raw materials of the soybeans, the gastrodia elata and the coffee are prepared by papain or compound enzyme Protamex enzymolysis.
4. The method for preparing bioactive peptide from soybean, coffee and gastrodia elata as claimed in any one of claims 1-3, wherein the bioactive peptide comprises the following components: the mass ratio of the soybean to the gastrodia elata to the caffeine and enkephalin is (1.3-3.1): (1.3-2.2): (1.8-4.1).
5. The method for preparing bioactive peptide from soybean, coffee and gastrodia elata as claimed in any one of claims 1-3, wherein the bioactive peptide comprises the following components: the mass ratio of the soybean, the gastrodia elata and the coffee compound peptide is (1.3-4.0): 0.42.
6. the method for preparing the bioactive peptide of the soybean, the coffee and the tall gastrodia tuber as claimed in claim 1, wherein the method comprises the following steps: and removing surface impurities from the composite micromolecular ionic peptide liquid, and centrifuging to obtain supernatant, namely the composite soybean gastrodia elata coffee micromolecular (ionic) peptide.
7. The application of the soybean, coffee and gastrodia elata bioactive peptide is characterized in that: blending with purified water according to the proportion of 1-5%, adding flavoring agent, plant preservative and purified water to obtain raw materials, and making into cosmetics, foods or medicines and health products.
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