CN114438155A - Preparation method and application of soybean, coffee and Chinese wolfberry bioactive peptide - Google Patents

Preparation method and application of soybean, coffee and Chinese wolfberry bioactive peptide Download PDF

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CN114438155A
CN114438155A CN202210030629.5A CN202210030629A CN114438155A CN 114438155 A CN114438155 A CN 114438155A CN 202210030629 A CN202210030629 A CN 202210030629A CN 114438155 A CN114438155 A CN 114438155A
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coffee
soybean
medlar
bioactive peptide
parts
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屈浩
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Yunnan Wenshan Kunqi Pharmaceutical Corp ltd
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Yunnan Wenshan Kunqi Pharmaceutical Corp ltd
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/01—Hydrolysed proteins; Derivatives thereof
    • A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The preparation method of the soybean, coffee and medlar bioactive peptide comprises the following steps of preparing raw materials of soybean, medlar and coffee: mixing 30-50 parts of soybean, 5-15 parts of medlar and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the medlar and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, and inactivating enzyme after enzymolysis; the application is as follows: blending with purified water according to the proportion of 1-5%, adding flavoring agent, plant preservative and purified water to obtain raw materials, and making into cosmetics, foods or medicines and health products. The situation of insufficient vital essence is well nourished. The long-term administration can also clear fire, improve eyesight, tranquilize mind, improve sleep quality, and has effects of caring skin and prolonging life; the health condition of the body is effectively improved; it also has effects in regulating immunity, inhibiting tumor growth and cell mutation, delaying aging, preventing fatty liver, regulating blood lipid and blood sugar, promoting hematopoiesis, invigorating qi, and tonifying kidney.

Description

Preparation method and application of soybean, coffee and Chinese wolfberry bioactive peptide
Technical Field
The invention relates to the field of preparation of bioactive peptides, in particular to a preparation method and application of bioactive peptides containing soybean, coffee and medlar.
Background
Bioactive Peptides (BAP) are a generic term for various Peptides ranging from dipeptides to complex linear, cyclic structures, composed of 20 natural amino acids in different compositions and arrangements in proteins, and are multifunctional compounds derived from proteins. The bioactive peptide has various human metabolism and physiological regulation functions, is easy to digest and absorb, has the effects of promoting immunity, hormone regulation, antibiosis, antivirus, blood pressure reduction, blood fat reduction and the like, and is a functional factor with the hottest research topic and great development prospect in the international food industry at present.
The existing bioactive peptide types do not take soybeans, medlar and coffee as raw materials, so that the bioactive peptide types are not rich enough, the nutritional ingredients are not diverse enough, and the requirements of consumers cannot be met.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a preparation method and application of a bioactive peptide containing soybean, coffee and medlar.
In order to achieve the aim of the invention, the invention adopts the specific scheme that:
a preparation method of soybean, coffee and medlar bioactive peptide comprises the following steps of raw materials of soybean, medlar and coffee: mixing 30-50 parts of soybean, 5-15 parts of medlar and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the medlar and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, carrying out enzymolysis, and then inactivating enzyme.
Furthermore, the protein raw materials of the soybeans, the medlar and the coffee are prepared by enzymolysis of pepsin and trypsin.
Further, the protein raw materials of the soybeans, the medlar and the coffee are prepared by papain or compound enzyme Protamex enzymolysis.
Further, the mass ratio of the soybean to the medlar to the caffeine and enkephalin is (1.3-3.1): (1.3-2.2): (1.8-4.1).
Further, the mass ratio of the soybean, medlar and coffee compound peptide is (1.3-4.0): 0.42.
further, removing surface impurities from the composite micromolecular ionic peptide liquid, centrifuging to obtain supernatant, and thus obtaining the composite soybean-medlar-coffee micromolecular (ionic) peptide.
The application of the bioactive peptide of soybean, coffee and medlar is to mix 1 to 5 percent of the ratio with purified water, add flavoring agent, plant preservative and purified water to obtain raw materials and prepare the raw materials into cosmetics, foods or medicines and health care products.
The invention has the beneficial effects that:
the invention prepares the composite plant micromolecule soybean, coffee and medlar peptide with a specific molecular weight range by a specific process, and the three or four of the composite plant micromolecule soybean, coffee and medlar peptide form a compound in a proper proportion, so that the composite plant micromolecule soybean, coffee and medlar peptide can well nourish the situation of insufficient essence and qi with a small using amount. The long-term administration can also clear fire, improve eyesight, tranquilize mind, improve sleep quality, and has effects of caring skin and prolonging life; the health-care food can be taken all the year round, so that the health condition is effectively improved; it also has effects in regulating immunity, inhibiting tumor growth and cell mutation, delaying aging, preventing fatty liver, regulating blood lipid and blood sugar, promoting hematopoiesis, invigorating qi, and tonifying kidney.
Detailed Description
The present invention is further described below by way of specific examples, but the present invention is not limited to only the following examples. Variations, combinations, or substitutions of the invention, which are within the scope of the invention or the spirit, scope of the invention, will be apparent to those of skill in the art and are within the scope of the invention.
A compound health promotion process using peptide health promotion function, coffee aroma and fructus Lycii health promotion effect is provided. Firstly, 30-70 parts of soybean, 5-15 parts of coffee and 15-40 parts of medlar are mixed to separate protein alkaline solution. Adding Alcalase protease into the alkaline solution obtained by separating soybean, coffee and medlar, performing enzymolysis, inactivating enzyme, centrifuging, and obtaining supernatant, namely the composite soybean coffee medlar peptide solution. Carrying out reflux ultrafiltration on the soybean, medlar, caffeine and enkephalin liquid in a greenhouse, and carrying out adsorption deodorization on activated carbon during the ultrafiltration. And performing series connection on 9 ultrafiltration membranes in the ultrafiltration operation to intercept molecular weight of 2500Da-4500Da and pressure of 0.2Mpa-1.2Mpa, treating for 5-20min, removing inorganic salt ions with relative molecular mass less than or equal to 2500Da, and collecting and filtering the soybean, coffee and Chinese wolfberry composite peptide liquid. And adding flavoring agents such as orange and strawberry liquid, fructo-oligosaccharide, xylitol, fruit sugar and the like in a greenhouse to improve the taste of the finished product of the compound peptide liquid. The oral liquid with health care and treatment functions is produced.
Example 1
The raw materials comprise the following components in parts by weight: 80 parts of compound soybean, medlar and coffee peptide, 2 parts of crystalline fructose and food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
s1, dissolving the composite soybean medlar coffee peptide in water (the mass ratio of materials to water is 1:10), adjusting the pH of the solution to 8.5 by using 1mol/L NaOH solution, adding alkaline protease for enzymolysis, then adjusting the pH to 7 by using 1mol/L HC1 solution, adding neutral protease for enzymolysis, adding 2500U/kg of the neutral protease for enzymolysis, inactivating the enzyme after the enzymolysis is finished, centrifuging, taking supernate, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 1000U to obtain filtrate with the molecular weight of less than 1000U, thereby obtaining the soybean medlar coffee composite oligopeptide concentrated solution.
S2, mixing soybean, medlar and coffee oligopeptides, dissolving the soybean, medlar and coffee oligopeptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 7 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzymes after the reaction is finished, centrifuging, taking supernate, dialyzing, precooling for 50min at the temperature of-10 ℃ to obtain a composite peptide concentrated solution, adding crystalline fructose and a food additive, and dissolving and mixing uniformly to obtain the plant peptide oral liquid.
Example 2
The raw materials comprise the following components in parts by weight: 75 parts of soybean, medlar and coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
s1, dissolving soybean and medlar coffee peptide in water (the mass ratio of materials to water is 1:10), adjusting the pH of the solution to 8.5 by using 1mol/L NaOH solution, adding alkaline protease for enzymolysis, then adjusting the pH to 7 by using 1mol/L HC1 solution, adding neutral protease for enzymolysis, adding 2500U/kg of the neutral protease for enzymolysis, inactivating enzyme after the enzymolysis is finished, centrifuging, taking supernate, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 1000U to obtain filtrate with the molecular weight of less than 1000U, thereby obtaining the soybean and medlar coffee composite oligomeric liquid.
S2, mixing soybean, medlar and coffee oligopeptides, dissolving the soybean, medlar and coffee oligopeptides in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 6 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzyme after the reaction is finished, centrifuging, taking supernate to obtain a compound peptide concentrated solution, adding crystalline fructose and a food additive, and dissolving and mixing uniformly to obtain the plant peptide oral liquid.
Comparative example 1
The raw materials comprise the following components in parts by weight: 75 parts of soybean, medlar and coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
adding crystalline fructose and food additive into the soybean, medlar and coffee compound peptide, dissolving and mixing uniformly to prepare the plant peptide oral liquid.
Comparative example 2
The raw materials comprise the following components in parts by weight: 75 parts of soybean, medlar and coffee compound peptide, 15 parts of crystalline fructose and 1.5 parts of food additive.
The food additive is prepared by mixing the following raw materials in parts by weight: 0.5 part of sweet orange essence, 0.5 part of bitter taste reducing essence, 0.4 part of mogroside and 0.05 part of stevioside.
A preparation method of plant peptide comprises the following steps:
mixing soybean and medlar coffee peptides, dissolving the mixture in water (the mass ratio of the materials to the water is 1:10), adjusting the pH to 6 by using 1mol/L HC1 solution, adding a-chymosin and flavourzyme for reaction, wherein the addition amount of the a-chymosin is 2000U/kg, the addition amount of the flavourzyme is 3000U/kg, inactivating enzyme after the reaction is finished, centrifuging, taking supernate, adding crystalline fructose and food additives, and dissolving and mixing uniformly to prepare the plant peptide liquid oral liquid.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (7)

1. A preparation method of soybean, coffee and medlar bioactive peptide is characterized in that the raw materials comprise soybean, medlar and coffee, and the specific method comprises the following steps: mixing 30-50 parts of soybean, 5-15 parts of medlar and 15-40 parts of coffee in proportion, firstly preparing a separated alkaline protein solution, separating the soybean, the medlar and the coffee, adding pepsin, trypsin, papain and cassava amylase into the alkaline solution, carrying out enzymolysis, and then inactivating enzyme.
2. The method for preparing the bioactive peptide of soybean, coffee and Chinese wolfberry fruit according to claim 1, wherein the bioactive peptide comprises the following components: the protein raw materials of the soybeans, the medlar and the coffee are prepared by enzymolysis of pepsin and trypsin.
3. The method for preparing the bioactive peptide of soybean, coffee and Chinese wolfberry fruit according to claim 1, wherein the bioactive peptide comprises the following components: the protein raw materials of the soybeans, the medlar and the coffee are prepared by papain or compound enzyme Protamex enzymolysis.
4. The method for preparing bioactive peptide from soybean, coffee and medlar according to any one of claims 1 to 3, wherein the bioactive peptide comprises the following components: the mass ratio of the soybeans to the medlar to the caffeine and enkephalin is (1.3-3.1): (1.3-2.2): (1.8-4.1).
5. The method for preparing bioactive peptide from soybean, coffee and medlar according to any one of claims 1 to 3, wherein the bioactive peptide comprises the following components: the mass ratio of the soybean, the medlar and the coffee compound peptide is (1.3-4.0): 0.42.
6. the method for preparing the bioactive peptide of soybean, coffee and Chinese wolfberry fruit according to claim 1, wherein the bioactive peptide comprises the following components: and removing surface impurities from the composite micromolecular ionic peptide liquid, and centrifuging to obtain supernatant, namely the composite soybean-medlar coffee micromolecular (ionic) peptide.
7. The application of the soybean, coffee and medlar bioactive peptide is characterized in that: blending with purified water according to the proportion of 1-5%, adding flavoring agent, plant preservative and purified water to obtain raw materials, and making into cosmetics, foods or medicines and health products.
CN202210030629.5A 2022-01-12 2022-01-12 Preparation method and application of soybean, coffee and Chinese wolfberry bioactive peptide Pending CN114438155A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1489923A (en) * 2003-09-16 2004-04-21 北京陶正生物工程技术有限公司 Method for producing soya protein bioactive peptide
CN104431208A (en) * 2014-12-29 2015-03-25 陕西天宝大豆食品技术研究所 Peptide coffee and preparation method thereof
CN106868081A (en) * 2016-12-13 2017-06-20 国肽生物科技(北京)有限公司 The extracting method of lbp peptide
CN107997186A (en) * 2018-01-31 2018-05-08 天津天肽生物科技有限公司 A kind of preparation method of matrimony vine polypeptide
CN110037163A (en) * 2019-04-24 2019-07-23 王书敏 A kind of preparation method of compound plant protein peptide
CN111358009A (en) * 2020-03-16 2020-07-03 赵秋萍 Plant peptide and preparation method and application thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1489923A (en) * 2003-09-16 2004-04-21 北京陶正生物工程技术有限公司 Method for producing soya protein bioactive peptide
CN104431208A (en) * 2014-12-29 2015-03-25 陕西天宝大豆食品技术研究所 Peptide coffee and preparation method thereof
CN106868081A (en) * 2016-12-13 2017-06-20 国肽生物科技(北京)有限公司 The extracting method of lbp peptide
CN107997186A (en) * 2018-01-31 2018-05-08 天津天肽生物科技有限公司 A kind of preparation method of matrimony vine polypeptide
CN110037163A (en) * 2019-04-24 2019-07-23 王书敏 A kind of preparation method of compound plant protein peptide
CN111358009A (en) * 2020-03-16 2020-07-03 赵秋萍 Plant peptide and preparation method and application thereof

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Application publication date: 20220506