M344034M344034
八、新型說明： 【新型所屬之技術領域】 • 本新型係提供一妹盖& 口口 .y 口養谷态防菌入侵的結構，即在容器 的頂盍内側上設一擔止緩，窄κ 會吸含於容器體盘頂蓋=門壤各器體内所蒸發的水氣’不 i入侵到培養容器内。 幻、、、田 【先前技術】 研究植物組織培養，斟 裨益無窮，雖然到近作物的育種及大量無性繁殖 看重，麸而研#亡門與年來，植物組織培養才重新被人 …、而研錢Η學問的人部越來越多，且研究 更=泛，這是因為借著它們，我們不但能 :組，、器官等的生長與分化，也可應用於營養生理: 恶形成及病理學上，因护六入 曲 ^ ¥殖方農學上的育種及大量無性 I殖方面，居有不可或缺的地位。 而前述的植物組織培養必需在無菌狀態下進行 =培養的組織材料必需是域的，不^ 菌 f呈’比如把殺菌過的種子培養在無菌狀態下，J = 成整株錢之植㈣，科此㈣所需制部份，如欲^ 野外植物體或組織來加以培養，則先經過表面^ 物另在組織培養過程中的污染來源包括：植物的本身手= 物所帶入的π染、操作室外在的污染源（ 滅菌設備或技術、人員、細鲦&在/ γ /、不凡王的 乾、事、雜及幻=、 操作臺上的I網不 W、瞒及到馬的存在）以及栽培過程中的 ί培室裡，培養瓶（罐）外和外界氣體交換時，、^有、、亏 木進入’心染機率取決於空氣中帶菌密度與培養瓶（罐 5 M344034 )空氣交換率。 所以，一般我們所常見的培養瓶（罐）的容器口處， 通常會以一頂蓋覆蓋，並在頂蓋上開設一透氣孔，且又在 透氣孔上覆設一透氣膜，藉此讓容器内外的空氣得以交換 ，但外界的細菌可被阻隔於外，同時容器口與頂蓋之間雖 然呈密合之狀態，但兩者密合處之間仍存有微小缝隙，當 培養瓶（罐）内之培養基所含的水份因溫度而蒸發時，待 外界溫度低於容器内之溫度時，原來蒸發的水氣即會在頂 蓋上凝結成水滴，直到水滴集聚至相當的重量時，就會向 下滴流，而仍凝結附著在頂蓋上的其它水滴，也可能會沿 著頂蓋表面而朝著較低處或任意方向流動，並當水滴流至 培養瓶（罐）口與頂蓋之間的密合處時，其間的縫隙則會 因虹吸原理，而將水滴吸入該缝隙内，導致培養瓶（罐） 口與頂蓋之間整個縫隙被凝結水所填滿。 如此，不但影響外界空氣無法經由該縫隙進行交換， 更會導致外界的細菌透過該缝隙間凝結水而進入培養瓶（ 罐）内，造成培養的植物遭到污染，而若發生前述的污 染時，將會造成下列的影響： (1) 生產成本的提高。 (2) 對植株造成傷害。 (3) 增加接種源及污染的機率。 【新型内容】 緣是，為了能有效地改善培養容器可能遭到外界細菌 入侵的問題，本新型所實行的技術手法，系令密合於容器 6 M344034 體上的頂蓋内側，在與容器體内壁相距一預定距離處，設 有一向下延伸的擋止緣，使與容器體之内侧斜壁面之間形 成阻斷虹吸作用之空隙者。 另，上述本新型之容器體頂端的開口周緣與頂蓋周緣 處，兩者係呈能相互密合的门形斷面者。 因此，藉由上述本新型之容器體的頂蓋上所設置的擋 止緣，讓容器體内所產生的蒸發水氣，在頂蓋上凝結成水 滴後，因擋止緣上端處與容器體内侧斜壁面之間形成一預 定較大之阻斷虹吸作用的空隙，進而讓凝結的水滴沿著擋 止緣而向下滴，而不會被吸入容器體與頂蓋之間縫隙中， 所以外界的細菌就不易沿著容器體與頂蓋之間的縫隙，而 入侵到培養容器内。 是以，本新型可藉由下列的實施例並配合附圖，而獲 得充分的了解，並據以實施： 【實施方式】 敬請參閱第一、二圖所示，本新型之培養容蓋係包括 一容器體1及一密合於容器體1上的頂蓋2，其中該容器 體1頂端的開口周緣處，設有呈门形斷面的杯緣11，並在 内側的稍低處再設一階部12，另該頂蓋2的周緣亦設有呈 相對而能密合於杯緣11上之门形斷面的蓋緣21，並使頂 蓋2的底面能夠密合於容器體1的階部12上，讓容器體1 與頂蓋2之間的密合處形成一更為彎曲縫隙；然後在頂蓋 2的底面稍内侧處，再設一向下伸的擋止緣22，並令該擋 止緣22的下端與容器體1之斜設的内壁面之間接觸，同時 7 M344034 也讓擂止緣22的外侧面與容器體1之内壁面之間形成一預 定的空隙；其次，在蓋緣21外侧邊的内側處再凸設一凸擋 部25，其目的係當頂蓋2覆蓋於容器體1上時，該凸擋部 25正好可以擋止於杯緣21之外侧面的下端。 是以，當外界與容器體1内產生較大的溫差時，容器 體1内之培養基的水份就會蒸發，並會在頂蓋2内侧凝結 成水滴，而水滴除了會向下滴流外，也會沿著頂蓋2底面 向任意方向流動，直至受到擋止緣22的阻擋後，水滴即會 聚集後而沿著擋止緣22向下滴，同時因為擋止緣22的外 侧面與容器體1的内側斜設壁面之間，形成一能夠阻斷虹 吸作用的空隙，所以水滴就不會被吸入容器體1與頂蓋2 之間密合的縫隙中，進而讓容器體1與頂蓋2之間的縫隙 保持乾燥，不但可提供培養容器内與外界空氣的交換，並 使外界的細菌就不易沿著該縫隙而入侵到培養容器内，所 以可減少培養容器受到外界污染源的污染。 其次，為增加外界與培養容器内之空氣的交換，亦可 如第三圖所示，在頂蓋2上開設透氣孔23，並在透氣孔23 上覆設的透氣膜24，進而讓培養容器之内外空氣的交換率 提南。 儘管，上述的實施例已對本新型有較佳之具體化的揭 露與詳述，皆為熟悉該項技術人士者所能清楚了解，而其 所做的各種型式及方法局部的改變，應都是沒有脫離本新 型精神與範疇。 【圖式簡單說明】 8 M344034 第一圖係本新型的立體示意圖。 第二圖係本新型的側面剖視圖。 第三圖係本新型頂蓋的另一實施例示意圖 【主要元件符號說明】 1 容器體 11 杯緣 12 2 頂蓋 21 蓋緣 22 23 透氣孔 24 透氣膜 25 階部 擔止緣 凸擋部Eight, new description: [New technology field] • This new type provides a sister cover & mouth mouth. y mouth corn-type anti-bacterial invasion structure, that is, a load on the inside of the top of the container is set to slow down, The narrow κ will be absorbed in the top of the container body cover = the water vapor evaporated in the body of the door is not invaded into the culture vessel.幻,,,田 [Previous technology] Research on plant tissue culture, endless benefits, although to the near crop breeding and a large number of asexual reproduction, bran research and death, and plant tissue culture has been re-applied... There are more and more people studying and studying, and the research is more general. This is because we can not only grow and differentiate groups, organs, but also nutrition and physiology: malignant formation and pathology. In school, because of the protection of the six into the song ^ ¥ agronomic breeding and a large number of asexuality, has an indispensable position. The above-mentioned plant tissue culture must be carried out under aseptic conditions = the cultured tissue material must be in the domain, and the bacteria f are 'for example, the sterilized seeds are cultured under aseptic conditions, and J = is the whole plant (4). In this case, if you want to cultivate the wild plant or tissue, you should first pass through the surface and the other sources of pollution in the tissue culture process include: the plant's own hand = the π dye brought by the object The source of pollution outside the operation (sterilization equipment or technology, personnel, fineness & in / γ /, the extraordinary king's dry, things, miscellaneous and magic =, the I network on the console is not W, 瞒 and the presence of the horse ) and in the cultivation room during the cultivation process, when the culture bottle (can) is exchanged with the outside air, the ^, and the loss of wood enters the 'heart dyeing rate depends on the density of the bacteria in the air and the culture bottle (can 5 M344034) air Exchange rate. Therefore, in general, the bottle mouth of the culture bottle (can) that we commonly use is usually covered with a top cover, and a vent hole is opened in the top cover, and a gas permeable membrane is placed on the vent hole, thereby allowing The air inside and outside the container can be exchanged, but the bacteria outside can be blocked, and the container mouth and the top cover are in a tight state, but there are still small gaps between the two tight places, when the culture bottle ( When the water contained in the medium in the tank is evaporated due to temperature, when the outside temperature is lower than the temperature in the container, the originally evaporated water will condense into water droplets on the top cover until the water droplets are concentrated to a considerable weight. , it will drip down, and other water droplets still attached to the top cover may also flow down the surface of the top cover or in any direction, and when the water drops to the mouth of the culture bottle (can) When it is in close contact with the top cover, the gap between them is sucked into the gap due to the siphon principle, and the entire gap between the culture bottle (can) port and the top cover is filled with condensed water. In this way, not only does the outside air not be exchanged through the gap, but also the outside bacteria can enter the culture bottle (can) through the condensed water between the gaps, causing the cultured plants to be contaminated, and if the aforementioned pollution occurs, Will have the following effects: (1) Increase in production costs. (2) Injury to plants. (3) Increase the probability of inoculation source and pollution. [New content] The reason is that in order to effectively improve the problem that the culture container may be invaded by external bacteria, the technical method of the present invention is to make the inner side of the top cover close to the container 6 M344034, and the container body The inner wall is spaced apart from the inner wall by a predetermined distance, and a downwardly extending stop edge is formed to form a gap between the inner side inclined wall surface of the container body and the siphon effect. Further, at the periphery of the opening of the top end of the container body of the present invention and the periphery of the top cover, both of them are gate-shaped sections which can be closely adhered to each other. Therefore, by the stopper edge provided on the top cover of the container body of the present invention, the evaporated water generated in the container body is condensed into water droplets on the top cover, and the upper end of the stopper edge is opposite to the container body. A predetermined larger gap for blocking the siphon is formed between the inner inclined wall surfaces, so that the condensed water drops downward along the stopping edge without being sucked into the gap between the container body and the top cover, so the outside world The bacteria are less likely to invade into the culture vessel along the gap between the container body and the top cover. Therefore, the present invention can be fully understood by the following embodiments and with reference to the accompanying drawings, and can be implemented accordingly: [Embodiment] Please refer to the first and second figures, the culture cover system of the present invention. The utility model comprises a container body 1 and a top cover 2 which is adhered to the container body 1. wherein the periphery of the opening of the top end of the container body 1 is provided with a cup edge 11 having a gate-shaped cross section, and is slightly lower at the inner side. The first step portion 12 is provided, and the periphery of the top cover 2 is also provided with a cover edge 21 which is opposite to the door-shaped cross section of the cup edge 11, and the bottom surface of the top cover 2 can be closely adhered to the container body. The step portion 12 of the first portion 12 forms a more curved gap between the container body 1 and the top cover 2; then, at a slightly inner side of the bottom surface of the top cover 2, a downwardly extending stop edge 22 is provided. And the lower end of the retaining edge 22 is in contact with the inclined inner wall surface of the container body 1, and 7 M344034 also forms a predetermined gap between the outer side surface of the retaining edge 22 and the inner wall surface of the container body 1; Next, a convex portion 25 is further protruded at the inner side of the outer side of the cover edge 21 for the purpose of covering the container body 1 when the top cover 2 is covered. The portion 25 can just stop at the lower end of the outer side of the cup edge 21. Therefore, when a large temperature difference is generated between the outside and the container body 1, the moisture of the culture medium in the container body 1 evaporates, and condensation water is formed on the inside of the top cover 2, and the water droplets are dripping downward. , it will also flow in any direction along the bottom of the top cover 2, until after being blocked by the blocking edge 22, the water droplets will gather and then drop down along the blocking edge 22, and because the outer side of the blocking edge 22 is The inner side of the container body 1 is obliquely disposed between the wall surfaces to form a gap capable of blocking the siphoning action, so that the water droplets are not sucked into the gap between the container body 1 and the top cover 2, thereby allowing the container body 1 and the top. The gap between the lids 2 is kept dry, which not only provides exchange of the outside air with the outside of the culture vessel, but also makes it difficult for bacteria outside to invade the culture vessel along the gap, so that the culture vessel can be reduced from the pollution of external sources. Secondly, in order to increase the exchange of the outside air with the air in the culture container, as shown in the third figure, a venting hole 23 is formed in the top cover 2, and a gas permeable membrane 24 is placed on the vent hole 23, thereby allowing the culture container to be The exchange rate of air inside and outside is raised. The above description of the embodiments of the present invention has been disclosed and described in detail as well as those skilled in the art, and the various changes in the various types and methods are not Leaving the spirit and scope of this new type. [Simple diagram of the diagram] 8 M344034 The first diagram is a three-dimensional diagram of the novel. The second drawing is a side cross-sectional view of the present invention. The third figure is a schematic view of another embodiment of the novel top cover. [Main component symbol description] 1 container body 11 cup edge 12 2 top cover 21 cover edge 22 23 vent hole 24 gas permeable membrane 25 step portion shoulder edge convex portion