TWI856468B - Smart controlled cultivation method to improve the growth of Antrodia cinnamomea - Google Patents

Abstract

A smart controlled cultivation method for improving the growth of Antrodia cinnamomea uses several light sources of specific different wavelengths to intermittently irradiate Antrodia cinnamomea cultivated on Antrodia cinnamomea basswood, and can instantly adjust the environmental humidity and temperature, and can also supplement nutrients to simulate the optimal environmental conditions for the growth of Antrodia cinnamomea in the wild. The analysis results of the growth and functional substances of Antrodia cinnamomea cultivated in the smart cultivation box are shown in Table 1. After 18 months of continuous cultivation, the weight of Antrodia cinnamomea in the experimental group increased by 26% compared with the control group, and the triterpenoid compounds increased by 12%, and the polysaccharides increased by 4%.

Description

Intelligent control cultivation method to improve the growth of Antrodia cinnamomea

The present invention relates to a smart controlled cultivation method for improving the growth of Antrodia cinnamomea, especially involving the cultivation of Antrodia cinnamomea, Ganoderma lucidum and even most plants, and particularly refers to a method that can successfully improve the growth of Antrodia cinnamomea and effectively increase the content of functional substances in Antrodia cinnamomea.

Current technology has proposed using LED specific light sources to increase the production of triterpenes or polysaccharides in Antrodia cinnamomea, but the use of a single specific light source cannot increase multiple functional substances at the same time. Another proposal is to use a specific culture solution for solid-state culture of Antrodia cinnamomea, and the cultured Antrodia cinnamomea ingredients are closer to wild species or artificial species cultured by basswood. Although Antrodia cinnamomea cultivated artificially can produce fruiting bodies when cultured in a solid state, and the ingredients are similar to those of wild species or artificial species cultured by basswood, the content of its functional substances is still different from the two.

In view of the various problems in the known technology, in order to be able to take into account and solve them, it is necessary to develop an invention that can solve the problem of the quantity and content of functional substances and the shortcomings of the previous technology.

The main purpose of the present invention is to overcome the above problems encountered by the prior art and provide a method for intelligent controlled cultivation that can successfully improve the growth of Antrodia cinnamomea and effectively increase the content of functional substances in Antrodia cinnamomea, so as to reduce the current situation of illegal mining of wild Antrodia cinnamomea.

Another purpose of the present invention is to provide a method for improving the production of Antrodia cinnamomea and thus reducing the overall price, so as to facilitate universal use. This method can be applied to the cultivation of Antrodia cinnamomea, Ganoderma lucidum and even most plants to improve the growth of Antrodia cinnamomea through intelligent controlled cultivation.

To achieve the above purpose, the present invention is a smart control cultivation method for improving the growth of Antrodia cinnamomea, which is applied to a smart control cultivation box device and executed on a smart cultivation box, a nutrient supply module, a temperature sensing and regulating module, a humidity sensing and regulating module, an LED light source module, a microprocessor module, and a cloud monitoring module in the smart control cultivation box device. The method at least includes the following steps: Step 1: In the smart cultivation box, A culture chamber is formed to place the culture medium inoculated with Antrodia cinnamomea strains, and to contain the nutrient source injected from the outside into the culture chamber for replenishment. The nutrient source comes from the microalgae fermentation liquid of several algae, and the carbon-nitrogen ratio is 1:5-1:6. The environmental temperature range of the culture chamber in the smart culture box is controlled between 24.5-25.5°C, and the humidity range is between 91-95%. Step 2: The temperature sensing and regulating module senses the temperature of the culture chamber. The temperature of the culture chamber is sensed by the humidity sensing and regulating module to generate an environmental temperature information, and the humidity of the culture chamber is sensed by the humidity sensing and regulating module to generate an environmental humidity information, and the environmental temperature information and the environmental humidity information are transmitted to the cloud monitoring module through the network by the microprocessing module; and step three: receiving the environmental temperature information and the environmental humidity information from the microprocessing module through the network, and the cloud monitoring module remotely controls the temperature and humidity information through the microprocessing module. The temperature sensing and regulating module and the humidity sensing and regulating module dynamically and instantly adjust the ambient temperature and humidity, or remotely control the LED light source module to intermittently irradiate the inoculated and cultured Antrodia cinnamomea with red light, green light, and blue light of specific wavelengths during the culture period, or remotely control the nutrient source supply module to fixedly replenish the nutrient source for the inoculated and cultured Antrodia cinnamomea during the culture period, or a combination thereof, to simulate the optimal environmental conditions for the growth of Antrodia cinnamomea in the wild.

In the above embodiments of the present invention, the nutrient source is derived from the microalgae fermentation liquid of green algae, brown algae and red algae.

In the above embodiment of the present invention, the culture medium in step 1 is a Cinnamomum tinctorium basswood, which is washed and sterilized at high temperature and high pressure, and then the Antrodia cinnamomea fungus is inoculated on the surface of the Cinnamomum tinctorium basswood by plane spore washing liquid inoculation.

In the above embodiment of the present invention, in step 3, the cloud monitoring module remotely controls the LED light source module to intermittently irradiate with red light of 6 μmol/m ² /s, green light of 10 μmol/m ² /s, and blue light of 9 μmol/m ² /s during the culture period.

In the above-mentioned embodiment of the present invention, in step 3, the cloud monitoring module remotely controls the nutrient source supply module to perform fixed replenishment of the nutrient source every three weeks during the culture period.

In the above embodiment of the present invention, the intelligent control culture chamber device further includes a carbon dioxide sensing and regulating module for sensing the carbon dioxide concentration in the culture chamber and generating environmental carbon dioxide concentration information accordingly. The microprocessing module receives the environmental carbon dioxide concentration information and transmits it to the cloud monitoring module via the network.

100: Intelligent control culture box device

1: Smart incubator

11: Cultivation room

12: Cinnamomum camphora

2: Nutrient supply module

21: Nutritional source

3: Temperature sensing and adjustment module

31: Thermometer

32: Heater

4: Humidity sensing and adjustment module

41: Humidity meter

42: Humidifier

5:LED light source module

6: Microprocessor module

61: Internet

7: Cloud monitoring module

71: Internet of Things Unit

8: Antrodia cinnamomea

9: Carbon dioxide sensing and regulating module

s11~s13: Steps

Figure 1 is a schematic diagram of the block structure of a specific embodiment of the intelligent control culture box device of the present invention.

Figure 2 is a schematic diagram of the steps of a specific embodiment of the intelligent control culture box device of the present invention.

Figure 3 is a three-dimensional schematic diagram of a specific embodiment of the intelligent control culture box device of the present invention.

Please refer to "Figures 1 to 3", which are respectively a block diagram of a specific embodiment of the intelligent control culture box device of the present invention, a step flow diagram of a specific embodiment of the intelligent control culture box device of the present invention, and a three-dimensional diagram of a specific embodiment of the intelligent control culture box device of the present invention. As shown in the figure: The present invention is a method for intelligent control culture to enhance the growth of Antrodia cinnamomea, which is applied to an intelligent control culture box device and executes an intelligent culture box 1, a nutrient supply module 2, a temperature sensing and regulating module 3, a humidity sensing and regulating module 4, an LED light source module 5, a microprocessor module 6, and a cloud monitoring module 7 in the intelligent control culture box device. The method at least includes the following steps:

Step s11, the smart culture box 1 forms a culture chamber 11 in the box, and the culture medium inoculated with Antrodia cinnamomea is placed, and the nutrient source 21 injected from the outside into the culture chamber 11 for replenishment can be contained. The nutrient source 21 comes from the microalgae fermentation liquid of several algae, and its carbon-nitrogen ratio is 1:5~1:6. The environmental temperature range of the culture chamber 11 in the smart culture box 1 is controlled between 24.5~25.5℃, and the humidity range is controlled between 91~95%; wherein, the culture medium is Antrodia cinnamomea basswood 12.

Step s12, the temperature sensing and regulating module 3 senses the temperature of the culture chamber 11 to generate an environmental temperature information, and the humidity sensing and regulating module 4 senses the humidity of the culture chamber 11 to generate an environmental humidity information, and transmits the environmental temperature information and the environmental humidity information to the cloud monitoring module 7 through the network 61 via the microprocessor module 6.

Step s13, receiving the ambient temperature information and the ambient humidity information from the microprocessor module 6 via the network 61, the cloud monitoring module 7 then remotely controls the temperature sensing and regulating module 3 and the humidity sensing and regulating module 4 through the microprocessor module 6 to dynamically and instantly adjust the ambient temperature and humidity, or remotely controls the LED light source module 5 to intermittently irradiate red light, green light, and blue light during the culture period, or remotely controls the nutrient source supply module 2 to fixedly replenish the nutrient source 21 during the culture period, or a combination thereof, to simulate the optimal environmental conditions for the growth of Antrodia cinnamomea in the wild.

The above-mentioned nutrient supply module 2 can be a sprayer, which is arranged on the smart culture box 1; the temperature sensing and regulating module 3 is arranged in the smart culture box 1, and includes a thermometer 31 and a heater 32; the humidity sensing and regulating module 4 is arranged in the smart culture box 1, and includes a humidity meter 41 and a humidifier 42; the LED light source module 5 is arranged in the culture chamber 11; the microprocessor module 6 can be a single board computer, which is respectively connected to the nutrient supply module 2, the temperature sensing and regulating module 3, the humidity sensing and regulating module 4 and the LED light source module 5; the cloud monitoring module 7 is provided with an Internet of Things unit 71.

The above-mentioned intelligent control culture chamber device 100 for improving the growth of Antrodia cinnamomea further includes a carbon dioxide sensing and regulating module 9, which is arranged in the culture chamber 11 and connected to the microprocessing module 6, for sensing the carbon dioxide concentration in the culture chamber 11 and generating environmental carbon dioxide concentration information accordingly, which is received by the microprocessing module 6 and transmitted to the cloud monitoring module 7 through the network 61.

In a preferred specific embodiment of the present invention, the first Antrodia cinnamomea smart culture box 1 of the present invention includes a temperature sensing and regulating module 3, a humidity sensing and regulating module 4, a carbon dioxide sensing and regulating module 9, and is equipped with an LED light source module 5 with specific wavelengths such as red, green, and blue, and is further provided with a nutrient supply module 2. All of the above equipment adjustments can be remotely controlled through a microprocessor module 6 and a cloud monitoring module 7. When used, several light sources with specific wavelengths are used to intermittently irradiate Antrodia cinnamomea 8 that have been cultured in Cinnamomum camphora 12, and the environmental humidity and temperature can be adjusted in real time, and nutrients can be supplemented 21 to simulate the optimal environmental conditions for the growth of Antrodia cinnamomea in the wild. The analysis results of the growth and functional substances of Antrodia cinnamomea cultured in the smart culture box are shown in Table 1. After 18 months of continuous cultivation, the weight of Antrodia cinnamomea in the experimental group increased by 26% compared with the control group, and the triterpenoid compounds increased by 12%, and the polysaccharides increased by 4%.

[Implementation Method 1] Inoculation of Antrodia cinnamomea strains

First, clean the Antrodia cinnamomea basswood 12, and then sterilize it at a high temperature and high pressure for 60 minutes at a sterilization temperature of 121°C. Then, inoculate the Antrodia cinnamomea fungus on the surface of the Antrodia cinnamomea basswood 12 by plane spore washing liquid inoculation, and control the ambient temperature at 25±0.5°C and the humidity at 93±2%. Place it in the culture chamber 11 of the smart culture box 1 for culture.

[Implementation Method 2] Control of Smart Cultivation Box

The smart culture box 1 can remotely control the temperature of the culture chamber 11 in the box at 25±0.5℃ and the humidity at 93±2% through the microprocessing module 6 and the cloud monitoring module 7, and can be assisted by an automatic humidifier 42 to increase the humidity, so that the temperature and humidity in the culture chamber 11 can be stably controlled for a long time, and during the culture period, the LED light source module 5 is controlled to emit red light at 6μmol/ ^m2 /s, green light at 10μmol/ ^m2 /s, and blue light at 9μmol/m2/ ^s. /s for intermittent irradiation, and at the same time, the nutrient source supply module 2 is configured to spray the microalgae fermentation liquid nutrient source 21 regularly every three weeks.

[Implementation Method Three] Nutritional Source Composition

The above-mentioned nutrient source 21 comes from the fermented microalgae of several algae such as green algae, brown algae and red algae, and its carbon-nitrogen ratio is 1:5~1:6.

Thus, the present invention aims to improve the growth of Antrodia cinnamomea to reduce the current situation of illegal exploitation of wild Antrodia cinnamomea. Therefore, a smart controlled culture box device for improving the growth of Antrodia cinnamomea is proposed. The present invention has successfully improved the growth of Antrodia cinnamomea and effectively increased the content of functional substances in Antrodia cinnamomea. By increasing the production of Antrodia cinnamomea, the overall price will be reduced, which is conducive to the universal use. It can be applied to the cultivation of Antrodia cinnamomea, Ganoderma lucidum and even most plants.

In summary, the present invention is a smart controlled cultivation method for improving the growth of Antrodia cinnamomea, which can effectively improve the various shortcomings of the conventional method, successfully improve the growth of Antrodia cinnamomea, and effectively increase the content of functional substances in Antrodia cinnamomea. By improving the production of Antrodia cinnamomea, the overall price will be reduced, which is conducive to the universal use. It can be applied to the cultivation of Antrodia cinnamomea, Ganoderma lucidum and even most plants, thereby making the production of the present invention more advanced, more practical, and more in line with the needs of users. It has indeed met the requirements for invention patent application, and a patent application is filed in accordance with the law.

However, the above is only a preferred embodiment of the present invention, and should not be used to limit the scope of implementation of the present invention; therefore, all simple equivalent changes and modifications made according to the scope of the patent application of the present invention and the content of the invention specification should still fall within the scope of the present invention patent.

100: Intelligent control culture box device

1: Smart incubator

11: Cultivation room

12: Cinnamomum camphora

2: Nutrient supply module

21: Nutritional source

3: Temperature sensing and adjustment module

31: Thermometer

32: Heater

4: Humidity sensing and adjustment module

41: Humidity meter

42: Humidifier

5:LED light source module

6: Microprocessor module

61: Internet

7: Cloud monitoring module

71: Internet of Things Unit

8: Antrodia cinnamomea

9: Carbon dioxide sensing and regulating module

Claims (6)

A smart control culture method for improving the growth of Antrodia cinnamomea is applied to a smart control culture box device and executed on a smart culture box, a nutrient supply module, a temperature sensing and regulating module, a humidity sensing and regulating module, an LED light source module, a microprocessor module, and a cloud monitoring module in the smart control culture box device. The method at least comprises the following steps: Step 1: forming a culture chamber in the smart culture box, placing The culture medium inoculated with Antrodia cinnamomea strains can contain nutrients injected from the outside into the culture chamber for replenishment. The nutrients come from microalgae fermentation liquid of several algae species, and the carbon-nitrogen ratio is 1:5~1:6. The environmental temperature range of the culture chamber in the smart culture box is controlled between 24.5~25.5℃, and the humidity range is between 91~95%. Step 2: The temperature sensing and regulating module senses the temperature of the culture chamber to generate The humidity sensing and regulating module senses the humidity of the culture chamber to generate the environmental humidity information, and transmits the environmental temperature information and the environmental humidity information to the cloud monitoring module through the network through the microprocessing module; and step three: receiving the environmental temperature information and the environmental humidity information from the microprocessing module through the network, and the cloud monitoring module remotely controls the temperature sensing and regulating module through the microprocessing module. The regulating module and the humidity sensing regulating module dynamically and instantly adjust the ambient temperature and humidity, or remotely control the LED light source module to intermittently irradiate the inoculated and cultured Antrodia cinnamomea with red light, green light, and blue light of specific wavelengths during the culture period, or remotely control the nutrient source supply module to fixedly replenish the nutrient source for the inoculated and cultured Antrodia cinnamomea during the culture period, or a combination thereof, to simulate the optimal environmental conditions for the growth of Antrodia cinnamomea in the wild. According to the intelligent controlled cultivation method for improving the growth of Antrodia cinnamomea described in Item 1 of the patent application, the nutrient source is the microalgae fermentation liquid from green algae, brown algae and red algae. According to the intelligent controlled cultivation method for improving the growth of Antrodia cinnamomea described in Item 1 of the patent application, in the step 1, the culture medium is a Antrodia cinnamomea basswood, which is washed and sterilized at high temperature and high pressure, and then the Antrodia cinnamomea strain is inoculated on the surface of the Antrodia cinnamomea basswood by plane spore washing liquid inoculation. According to the intelligent controlled cultivation method for improving the growth of Antrodia cinnamomea described in Item 1 of the patent application, in step 3, the cloud monitoring module remotely controls the LED light source module to intermittently irradiate with red light of 6 μmol/m ² /s, green light of 10 μmol/m ² /s, and blue light of 9 μmol/m ² /s during the cultivation period. According to the intelligent controlled cultivation method for improving the growth of Antrodia cinnamomea described in Item 1 of the patent application, in step 3, the cloud monitoring module remotely controls the nutrient supply module to perform fixed replenishment of the nutrient source every three weeks during the cultivation period. According to the intelligent control cultivation method for improving the growth of Antrodia cinnamomea described in Item 1 of the patent application, the intelligent control cultivation chamber device further includes a carbon dioxide sensing and regulating module for sensing the carbon dioxide concentration in the cultivation chamber and generating environmental carbon dioxide concentration information accordingly, which is received by the microprocessor module and transmitted to the cloud monitoring module via the network.

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