TWI812941B - Fluorescence detection constant temperature oscillating mixer - Google Patents
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 26
- 239000012472 biological sample Substances 0.000 claims abstract description 28
- 230000005540 biological transmission Effects 0.000 claims abstract description 19
- 230000010355 oscillation Effects 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 10
- 230000004308 accommodation Effects 0.000 claims description 5
- 238000001514 detection method Methods 0.000 abstract description 14
- 230000001427 coherent effect Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 4
- 241000700605 Viruses Species 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005485 electric heating Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
一種螢光偵測恆溫振盪混勻儀，主要係包含一機體及設於該機體之盤座、複數之螢光偵測器。其中，該機體係設有溫度控制裝置及一傳動件；該盤座係設有複數間隔排列之承架，其底部係樞接於傳動件；該等螢光偵測器係對應於盤座之承架。俾將容置有待檢測生物性樣本之微量管置於盤座之承架，藉由傳動件的帶動，使盤座產生快速轉動及來回轉動，將較不易溶解之生物性樣本以離心力甩出及來回攪動，以達到確實混勻之目的，並藉由螢光偵測器偵測螢光變化，使偵測曲線得以連貫，進而確保螢光偵測之精準性。A fluorescence detection constant-temperature oscillation mixer mainly includes a machine body, a tray installed on the machine body, and a plurality of fluorescence detectors. Among them, the machine system is equipped with a temperature control device and a transmission component; the tray base is provided with a plurality of spaced-apart supports, the bottom of which is pivotally connected to the transmission component; the fluorescent detectors are corresponding to the tray base Bearing frame. In order to place the microtube containing the biological sample to be tested on the holder of the tray, driven by the transmission component, the tray will rotate rapidly and back and forth, and the biological samples that are less soluble will be thrown out by centrifugal force. Stir back and forth to achieve the purpose of accurate mixing, and use the fluorescence detector to detect changes in fluorescence to make the detection curve coherent, thereby ensuring the accuracy of fluorescence detection.
Description
本發明係有關一種螢光偵測恆溫振盪混勻儀，尤指一種可供將較不易溶解之生物性樣本快速轉動及來回攪動，並藉由螢光偵測器偵測螢光變化，使偵測曲線得以連貫，進而確保螢光偵測之精準性之螢光偵測恆溫振盪混勻儀。The present invention relates to a fluorescence detection constant temperature oscillation mixer, in particular, a device that can quickly rotate and stir back and forth biological samples that are difficult to dissolve, and detect changes in fluorescence through a fluorescence detector. Fluorescence detection constant temperature oscillation mixer that makes the measurement curve consistent and ensures the accuracy of fluorescence detection.
按；近年來，醫學實驗室為提升檢驗品質的一致性，改善傳統人工檢驗方式的限制，因此於分子醫學的檢驗領域中，不斷的有自動化儀器設備被成功開發且實施運用。而通常分子醫學的檢驗過程中，係將待測生物樣本密封於微量管中，以供螢光偵測儀導入300-800nm波長之各種光源，激發管內生物樣本所含的螢光物質，同時可導出放射螢光，以供進行光學偵測之用。Press; In recent years, in order to improve the consistency of testing quality and improve the limitations of traditional manual testing methods, medical laboratories have continuously successfully developed and implemented automated instruments and equipment in the field of molecular medicine testing. In the usual testing process of molecular medicine, the biological sample to be tested is sealed in a microtube, so that the fluorescence detector can introduce various light sources with wavelengths of 300-800nm to excite the fluorescent substances contained in the biological sample in the tube. Emission fluorescence can be exported for optical detection.
習知於螢光偵測過程中，需先將微量管內之待測生物樣本予以攪動使其混勻，再藉由適當的轉動，於轉動之同時由螢光偵測儀偵測螢光值的變化（酵素反應），惟由於其混勻過程與轉動過程並非連續動作，因此會導致偵測曲線不連貫，相對可能造成偵測結果的不精準性。It is commonly known that in the process of fluorescence detection, the biological sample to be tested in the microtube needs to be stirred and mixed first, and then through appropriate rotation, the fluorescence value is detected by the fluorescence detector while rotating. changes (enzyme reaction), but because the mixing process and the rotating process are not continuous actions, it will cause the detection curve to be incoherent, which may lead to inaccuracy in the detection results.
申請人有鑑於此，經不斷研究、實驗，遂萌生設計一種螢光偵測恆溫振盪混勻儀，可供將較不易溶解之生物性樣本快速轉動及來回攪動，並藉由螢光偵測器偵測螢光變化，進而確保螢光偵測之精準性。In view of this, after continuous research and experiments, the applicant came up with the idea of designing a fluorescence detection constant temperature oscillation mixer, which can quickly rotate and stir back and forth biological samples that are less easily dissolved, and use the fluorescence detector to Detect fluorescence changes to ensure the accuracy of fluorescence detection.
本發明之主要目的，即在提供一種螢光偵測恆溫振盪混勻儀，可供將較不易溶解之生物性樣本快速轉動及來回攪動，將較不易溶解之生物性樣本以離心力甩出，並藉由螢光偵測器偵測螢光變化，使偵測曲線得以連貫，進而確保螢光偵測之精準性。The main purpose of the present invention is to provide a fluorescence detection constant temperature oscillation mixer that can quickly rotate and stir back and forth biological samples that are less easily soluble, and throw out the less easily soluble biological samples with centrifugal force, and The fluorescent detector detects changes in fluorescence to make the detection curve consistent, thereby ensuring the accuracy of fluorescence detection.
前述之螢光偵測恆溫振盪混勻儀，係包含一機體及設於該機體之盤座、複數之螢光偵測器。其中，該機體係設有溫度控制裝置及一傳動件；該盤座係設有複數間隔排列之承架，其底部係樞接於傳動件；該等螢光偵測器係對應於盤座之承架。俾將容置有待檢測生物性樣本之微量管置於盤座之承架，藉由傳動件的帶動，使盤座產生快速轉動及來回轉動，將較不易溶解之生物性樣本以離心力甩出及來回攪動，以達到確實混勻之目的，並藉由螢光偵測器偵測螢光變化，使偵測曲線得以連貫，進而確保螢光偵測之精準性。The above-mentioned fluorescence detection constant temperature oscillation mixer includes a body, a tray installed on the body, and a plurality of fluorescence detectors. Among them, the machine system is equipped with a temperature control device and a transmission component; the tray base is provided with a plurality of spaced-apart supports, the bottom of which is pivotally connected to the transmission component; the fluorescent detectors are corresponding to the tray base Bearing frame. In order to place the microtube containing the biological sample to be tested on the holder of the tray, driven by the transmission component, the tray will rotate rapidly and back and forth, and the biological samples that are less soluble will be thrown out by centrifugal force. Stir back and forth to achieve the purpose of accurate mixing, and use the fluorescence detector to detect changes in fluorescence to make the detection curve coherent, thereby ensuring the accuracy of fluorescence detection.
前述之螢光偵測恆溫振盪混勻儀，其中該機體上方係設有一槽部，於該槽部設有溫度控制裝置，該盤座係設於機體之槽部，藉由溫度控制裝置提供恆定之溫度。In the aforementioned fluorescence detection constant temperature oscillation mixer, a groove is provided above the body, and a temperature control device is provided in the groove. The tray is located in the groove of the body, and the temperature control device provides a constant temperature. the temperature.
前述之螢光偵測恆溫振盪混勻儀，其中該機體之槽部體１係概呈梯形，其一端係設為半圓狀，另端係設為水平狀，由該半圓狀端兩側向外延伸適當寬度，以形成對稱之兩個容置空間，以供容置螢光偵測器。In the above-mentioned fluorescence detection constant temperature oscillation mixer, the tank body 1 of the body is generally trapezoidal, with one end set in a semicircular shape and the other end set in a horizontal shape, outward from both sides of the semicircular end. Extend the appropriate width to form two symmetrical accommodation spaces for accommodating fluorescent detectors.
前述之螢光偵測恆溫振盪混勻儀，其中該盤座所設之承架，係由盤座以適當之傾斜角度向上延伸，於該等承架分別設有通孔，以供插置容置有待檢測生物性樣本之微量管。In the aforementioned fluorescence detection constant temperature oscillation mixer, the racks provided on the tray extend upward from the tray at an appropriate tilt angle, and through holes are respectively provided in the racks for insertion and accommodation. Set up microtubes containing biological samples to be tested.
請參閱圖1及圖2，並配合圖3，係為本發明之立體圖及部分剖視圖。如圖所示，本發明係包含一機體１及設於該機體１之盤座２、複數之螢光偵測器３。其中，該機體１係設有一傳動件１１，上方係設有一槽部１２，於該槽部１２設有溫度控制裝置１３。於本實施例，該槽部１係概呈梯形，其一端係設為半圓狀，另端係設為水平狀，由該半圓狀端兩側向外延伸適當寬度，以形成對稱之兩個容置空間。該溫度控制裝置１３係為可提供恆定溫度之電熱片或電熱元件。Please refer to Figures 1 and 2, together with Figure 3, which are a perspective view and a partial cross-sectional view of the present invention. As shown in the figure, the present invention includes a body 1, a tray 2 provided on the body 1, and a plurality of fluorescent detectors 3. Among them, the machine body 1 is provided with a transmission member 11, a groove portion 12 is provided above, and a temperature control device 13 is provided in the groove portion 12. In this embodiment, the groove portion 1 is generally trapezoidal, with one end being semicircular and the other end being horizontal. An appropriate width extends outward from both sides of the semicircular end to form two symmetrical volumes. placement space. The temperature control device 13 is an electric heating plate or electric heating element that can provide constant temperature.
該盤座２係設於機體１之槽部１２，其底部係樞接於傳動件１１，藉由傳動件１１帶動其產生快速轉動及來回攪動之動作，其係設有複數間隔排列之承架２１，該等承架２１係由盤座２的周緣以適當之傾斜角度向上延伸，於該等承架２１分別設有通孔２１１。The tray base 2 is installed in the groove portion 12 of the machine body 1, and its bottom is pivotally connected to the transmission member 11, which is driven by the transmission member 11 to produce rapid rotation and back-and-forth stirring. It is provided with a plurality of spaced-apart supports. 21. The supports 21 extend upward from the periphery of the tray base 2 at an appropriate inclination angle, and are respectively provided with through holes 211 on the supports 21 .
該等螢光偵測器３，係對應於盤座２之承架２１。於本實施例，該等螢光偵測器３係設於機體１槽部１２之兩個容置空間，以適當之偵測角度對應於盤座２之承架２１，並以三角狀之遮板１４固定於機體１槽部１２上方構成適當的遮蔽。The fluorescent detectors 3 correspond to the bracket 21 of the tray base 2 . In this embodiment, the fluorescent detectors 3 are installed in two accommodation spaces in the groove portion 12 of the body 1, corresponding to the bracket 21 of the tray 2 at appropriate detection angles, and covered with triangular shields. The plate 14 is fixed above the groove 12 of the body 1 to form a suitable shield.
藉由前述構件的組合，構成螢光偵測恆溫振盪混勻儀。俾將容置有血液、體液、動物組織、植物組織、病毒、游離核酸，以及真核或原核細胞等待檢測生物性樣本之微量管置於盤座之承架，藉由傳動件１１的帶動，使盤座２產生快速轉動及來回轉動，將較不易溶解之生物性樣本以離心力甩出及來回攪動，以達到確實混勻之目的，並藉由螢光偵測器３偵測螢光變化，使偵測曲線得以連貫，進而確保螢光偵測之精準性。Through the combination of the aforementioned components, a fluorescence detection constant temperature oscillation mixer is constructed. In order to place the microtubes containing blood, body fluids, animal tissues, plant tissues, viruses, free nucleic acids, and eukaryotic or prokaryotic cells waiting for detection of biological samples on the tray holder, driven by the transmission member 11, The tray 2 is caused to rotate rapidly and back and forth, and the less soluble biological samples are thrown out by centrifugal force and stirred back and forth to achieve the purpose of accurate mixing, and the fluorescent detector 3 is used to detect changes in fluorescence. This makes the detection curve coherent and ensures the accuracy of fluorescence detection.
請同時參閱圖4及圖5，係為本發明之使用狀態圖。如圖所示，本發明於使用時，係將容置有血液、體液、動物組織、植物組織、病毒、游離核酸，以及真核或原核細胞等待檢測生物性樣本之微量管４，插置於於盤座２之承架２１所設之通孔２１１，使一個或一個以上之微量管４分別固定於不同之承架２１。Please refer to Figure 4 and Figure 5 at the same time, which are diagrams of the usage status of the present invention. As shown in the figure, when the present invention is used, microtubes 4 containing blood, body fluids, animal tissues, plant tissues, viruses, free nucleic acids, and eukaryotic or prokaryotic cells awaiting detection of biological samples are inserted into The through hole 211 provided in the support frame 21 of the tray base 2 allows one or more microtubes 4 to be fixed to different supports 21 respectively.
請參閱圖6，係為本發明之動作狀態圖。如圖所示，本發明將容置有待檢測生物性樣本之微量管４分別置於盤座２之承架２１後,，係藉由機體１之傳動件１１帶動該盤座２，使盤座２快速的旋轉。其中，由於該盤座２的承架２１係以適當之傾斜角度向上延伸，俾當盤座２快速旋轉時得以產生一定之離心力，進而將微量管４內之待檢測生物性樣本向外甩動，使較不易溶的待檢測生物性樣本達到混勻之目的。Please refer to Figure 6, which is an action state diagram of the present invention. As shown in the figure, in the present invention, the microtubes 4 containing the biological samples to be detected are placed on the rack 21 of the tray 2, and the tray 2 is driven by the transmission member 11 of the body 1, so that the tray 2 is 2 Rapid rotation. Among them, because the support 21 of the tray 2 extends upward at an appropriate tilt angle, a certain centrifugal force can be generated when the tray 2 rotates rapidly, thereby swinging the biological sample to be detected in the microtube 4 outwards. , to achieve the purpose of mixing the less soluble biological samples to be tested.
請參閱圖7，係為本發明之另一動作狀態圖。敬請配合圖3，如圖所示，本發明於前述使盤座２快速旋轉，使微量管４內之待檢測生物性樣本甩動後，係再藉由機體１之傳動件１１帶動該盤座２，使盤座２來回的轉動，進而使微量管４內之待檢測生物性樣本構成來回的攪動，以達到確實混勻之目的。Please refer to Figure 7, which is another action state diagram of the present invention. Please cooperate with Figure 3. As shown in the figure, in the present invention, after the disk holder 2 is rapidly rotated to shake the biological sample to be detected in the microtube 4, the disk is then driven by the transmission member 11 of the body 1. The base 2 is rotated back and forth, thereby causing the biological sample to be detected in the microtube 4 to be stirred back and forth to achieve the purpose of accurate mixing.
前述本發明於快速旋轉及來回攪動的動作中，設於機體１之溫度控制裝置１３係提供一恆定的溫度，使待檢測生物性樣本得以於恆溫狀態下完成混勻之流程。而當快速旋轉及來回攪動動作完成後，係藉由設於機體１槽部１２之兩個容置空間之螢光偵測器３，對微量管４內之待檢測生物性樣本進行螢光變化偵測（酵素反應）。由於該待檢測生物性樣本於混勻過程與螢光偵測過程，係於不中斷之連續動作中所完成，從而使偵測曲線得以連貫，進而可確保螢光值偵測之精準性。In the aforementioned invention, during the rapid rotation and back-and-forth stirring action, the temperature control device 13 provided in the body 1 provides a constant temperature, so that the biological sample to be detected can complete the mixing process at a constant temperature. After the rapid rotation and back-and-forth stirring are completed, the fluorescence detector 3 provided in the two accommodation spaces of the groove 12 of the body 1 changes the fluorescence of the biological sample to be detected in the microtube 4 Detection (enzyme reaction). Since the mixing process and fluorescence detection process of the biological sample to be detected are completed in uninterrupted continuous actions, the detection curve can be coherent, thereby ensuring the accuracy of fluorescence value detection.
前述實施例，僅為說明本發明之較佳實施方式，而非限制本發明之範圍，凡經由些微修飾、變更，仍不失本發明之要義所在，亦不脫本發明之精神範疇。The foregoing embodiments are only for illustrating the preferred embodiments of the present invention, rather than limiting the scope of the present invention. Slight modifications and changes may be made without losing the gist of the present invention and without departing from the spirit and scope of the present invention.
綜上所述，本發明以設有傳動件之機體，配合樞接於傳動件，且設有複數間隔排列承架之盤座，構成螢光偵測恆溫振盪混勻儀。以供將較不易溶解之生物性樣本快速轉動及來回攪動，將較不易溶解之生物性樣本以離心力甩出，並藉由螢光偵測器偵測螢光變化，使偵測曲線得以連貫，進而確保螢光偵測之精準性。為一實用之設計，誠屬一俱新穎性之發明，爰依法提出專利之申請，祈 鈞局予以審查，早日賜准專利，至感德便。To sum up, the present invention uses a body equipped with a transmission element, which is pivotally connected to the transmission element, and is provided with a plurality of tray seats arranged at intervals to form a fluorescence detection constant temperature oscillation mixer. It is used to quickly rotate and stir back and forth the less easily soluble biological samples, throw out the less easily soluble biological samples with centrifugal force, and detect the fluorescence changes through the fluorescence detector, so that the detection curve can be consistent. This ensures the accuracy of fluorescence detection. It is a practical design and is truly a novel invention. I submit a patent application in accordance with the law and pray that the Bureau will review it and grant the patent as soon as possible.
1:機體 11:傳動件 12:槽部 13:溫度控制裝置 14:遮板 2:盤座 21:承架 211:通孔 3:螢光偵測器 4:微量管 1: Body 11: Transmission parts 12: Groove 13:Temperature control device 14: Shutter 2: Tray seat 21:bearing frame 211:Through hole 3: Fluorescence detector 4: Microtube
圖1係本發明之立體圖。 圖2係本發明之另一立體圖。 圖3係本發明之部分剖視圖。 圖4及圖5係本發明之使用狀態圖。 圖6係本發明之動作狀態圖。 圖7係本發明之另一動作狀態圖。 Figure 1 is a perspective view of the present invention. Figure 2 is another perspective view of the present invention. Figure 3 is a partial cross-sectional view of the present invention. Figures 4 and 5 are diagrams of the usage state of the present invention. Figure 6 is an operation state diagram of the present invention. Figure 7 is another operating state diagram of the present invention.
1:機體 1: Body
11:傳動件 11: Transmission parts
12:槽部 12: Groove
14:遮板 14: Shutter
2:盤座 2: Tray seat
21:承架 21:bearing frame
211:通孔 211:Through hole
3:螢光偵測器 3: Fluorescence detector
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Citations (9)
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|---|---|---|---|---|
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| TW200710381A (en) * | 2005-07-05 | 2007-03-16 | 3M Innovative Properties Co | Heating element for a rotating multiplex fluorescence detection device |
| US20150132185A1 (en) * | 2010-07-14 | 2015-05-14 | Chromoplas Pty Ltd | Multi vessel ring |
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| CN212188855U (en) * | 2020-02-28 | 2020-12-22 | 江苏美诚生物科技有限公司 | Auxiliary equipment for assisting blood sample judgment and analysis |
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| Publication number | Publication date |
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| TW202242387A (en) | 2022-11-01 |
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