TWI780480B - Magnetic extraction type biological sample collection method and system for water - Google Patents

Abstract

A biological sample collection method includes: arranging a magnetic extraction sample collecting device in a water tank unit or a sample water, with combining the magnetic extraction sample collecting device with the water tank unit; providing a ultra-sound generator in the sample water for generating ultra sound waves therein; transmitting a ultra sound wave to the sample water for shocking at least one material which is dropped in ultra-sound processed water; and utilizing the magnetic extraction sample collecting device to magnetically attract biological samples in the ultra-sound processed water to extract at least one attached biological sample.

Description

Magnetic suction type water body biometric sample collection method and system

The present invention relates to a biological sample collection [biological sample collection] method and system thereof; in particular to a magnetic extraction type [magnetic extraction type] biological sample collection method and system thereof; more particularly to an ultrasonic type 〔ultrasound type〕A collection method and system for biological characteristics samples of water bodies.

For example, the conventional magnetic bead biosensing method and its device, for example: US Patent Publication No. US-20090206825 "Excitation and Measurement Method for a Magnetic Biosensor" invention patent application, which discloses a digital magnetic sensor system. The digital magnetic sensor system includes at least one digital magnetic sensor element and a processor.

Continuing from the above, the digital magnetic sensor element of the aforementioned US-20090206825 includes a magnetic element, a bit line and a word line, and the word line is oriented to be orthogonal to the bit line, and the A digital magnetic sensor element is used to sense the presence of a magnetic bead proximate to a top surface. The processor communicates with the digital magnetic sensor element, and the processor is used for executing program data, and when executing the program data, a method for sensing the presence of magnetic beads is generated.

As mentioned above, when the processor of the aforementioned US-20090206825 executes the program data, its execution items include: measuring an initial state of the magnetic element of the digital magnetic sensor element; controlling to provide a predetermined current pulse to the The bit line of the digital magnetic sensor element and each of the word lines, the current pulse can switch the magnetic element of the digital magnetic sensor element After providing the current pulse, measuring a first state of the magnetic element of the digital magnetic sensor element; and comparing the measurement between the first state and the initial state, and providing a comparison result.

Another conventional magnetic bead biosensing method and its device, for example: US Patent Publication No. US-20160146798 "Biosensor Based on the Measurements of Clustering Dynamics of Magnetic Particles Using a Double Pass Setup" invention patent application, which discloses A biosensor based on dynamic measurement of magnetic particle clusters with dual-pass configuration and its operating method. The biosensor is a biosensor for optical detection of Brownian relaxation dynamics of magnetic particles measured by light transmission.

As mentioned above, the biosensor of the aforementioned US-20160146798 includes several magnetic particles, and is functionalized with biological ligands of the several magnetic particles, so that it detects a target analyte in a sample , and the biosensor can be implemented in the configuration of a magnetic disk and an optical pickup head.

Another conventional magnetic bead biosensing method and its device, for example: US Patent Publication No. US-20180067175 "Biomolecule Magnetic Sensor" invention patent application, which discloses a biomolecular magnetic sensor, and the biomolecular magnetic The sensor is used for sensing at least one biomolecule to be tested, and several nano magnetic beads are attached to the biomolecule to be tested. The biomolecular magnetic sensor includes an adsorption piece and a magnetic force line generator.

As mentioned above, the adsorption sheet of the aforementioned US-20180067175 is used to attach the nano magnetic beads, and the magnetic force generator is used to generate several first magnetic force lines, and at least one of the first magnetic force lines passes through the nanometer along a first direction. magnetic beads, so that the magnetic nano beads induce one of the first lines of magnetic force to generate several second lines of magnetic force. The magnetic flux generator is located between the adsorption sheet and at least one magnetic sensing element in the first direction, and the magnetic sensing element and the adsorption sheet have a first offset and a second offset in different directions. displacement.

As above, the magnetic sensing element of the aforementioned US-20180067175 is disposed on at least one second magnetic force line, and the magnetic sensing element senses a magnetic field component of at least one second magnetic force line in a second direction. The magnetic sensing element is arranged at a position where the magnetic field component of at least one of the second magnetic force lines in the second direction is not zero, and the first direction and the second direction are different, and the magnetic sensing element and the adsorption piece are in the position where The second direction has the second offset.

Another conventional ultrasonic biosensing method and its system, for example: US Patent Publication No. US-20100167412 "Sensor System for Determining Concentration of Chemical and Biological Analyzes" invention patent application, which discloses a method for measuring chemical and biological Sensing system for concentration of biological analytes. The sensor system includes a disposable reagent delivery piece, at least one light source, at least one light detector and an electronic circuit component, and an ultrasonic generator is embedded in the sensor system.

Based on the foregoing, the disposable reagent transporter of US-20100167412 can be detachably assembled into a liquid disposal unit, and the liquid disposal unit can pump liquid back into the disposable reagent transporter. The photodetector can generate an electronic signal response indicative of light passing through or generating light from an interior space of the disposable reagent transport member, and the electronic circuit member is used for processing, storing and sending the electronic signal in response, and the electronic circuit component controls the light source.

Another conventional ultrasonic biosensing method and its system, for example: US Patent No. US-7972866 "Biosensor and ultrasonic method of making a biosensor" invention patent, which discloses a test strip for testing fluid samples And a method for manufacturing a biosensor.

As mentioned above, the test strip for testing fluid samples of the aforementioned US-7972866 includes a sample cavity and an ultrasonic dispersion reagent layer. The sample chamber is configured to receive a fluid sample, and the ultrasonic dispersion test The agent layer is configured on at least one surface of the sample chamber.

As mentioned above, the method for manufacturing a biosensor of the aforementioned US-7972866 includes: providing a substrate, and the substrate has a sample receiving area, and the sample receiving area is used to receive a sample; distributing the liquid substance on at least one surface of the sample receiving area; and vibrating the base material with an ultrasonic wave so as to quickly and uniformly disperse the liquid substance on at least one surface of the sample receiving area.

Another conventional ultrasonic biosensing method and its device, for example: Republic of China Patent Announcement No. TW-202024630 "Magnetic Biosensing Device and Biosensing Method" invention patent application, which discloses a magnetic biosensing device. The magnetic bio-sensing device includes a channel, a magnetic sensor and an acoustic wave emitter.

As mentioned above, the magnetic sensor of the aforementioned TW-202024630 is correspondingly arranged on the flow channel, and the sound wave transmitter includes two sound wave generating units, and the flow channel is arranged between the two sound wave generating units. The acoustic wave emitter is used to generate an ultrasonic standing wave in the flow channel, and the magnetic sensor corresponds to a node position of the ultrasonic standing wave.

However, the aforementioned U.S. Patent Publications No. US-20090206825, No. US-20160146798, No. US-20180067175, No. US-20100167412, U.S. Patent Publication No. US-7972866 and the Republic of China Patent Publication No. TW-202024630 The biosensing method and its system are not suitable for water biometric sample collection technology, so there must be a potential demand for further improvement of the overall structure.

The aforementioned U.S. Patent Publications No. US-20090206825, No. US-20160146798, No. US-20180067175, No. US-20100167412, U.S. Patent Publication No. US-7972866 and the Republic of China Patent Publication No. TW-202024630 are invention patents And the patent application for invention is only for the reference of the technical background of the present invention and to illustrate the current state of technological development, and it is not intended to limit the scope of the present invention.

In view of this, in order to meet the above needs, the present invention provides a magnetic suction type water body biological characteristic sample collection method and its system, which configures a magnetic suction type biological sample collection device in a water body container unit or a water body to be collected samples, And an ultrasonic generating device is combined with the sample water body to be collected, and at least one of the water body to be treated is processed by ultrasonic vibration using the ultrasonic generating device, and then collected by the magnetic suction type biological sample collection device At least one ultrasonically treated water body of the sample water to be collected is adsorbed to facilitate the collection of at least one adsorbed biological sample by the magnetic suction type biological sample collection device, so as to improve the problems of various conventional biological sensing methods and systems thereof.

The main purpose of the preferred embodiment of the present invention is to provide a magnetic suction type water body biological characteristic sample collection method and system thereof, which configures the water body of a magnetic suction type biological sample collection device in a water body container unit or a sample to be collected, and An ultrasonic generating device is combined with the sample water body to be collected, and the ultrasonic generating device is used to perform ultrasonic vibration treatment on at least one of the sample water body to be treated, and then the magnetic suction type biological sample collection device is used for collection and adsorption treatment At least one of the water bodies to be sampled has been ultrasonically treated to facilitate the collection of at least one adsorbed biological sample by the magnetic suction biological sample collection device, so as to achieve the purpose of improving the magnetic suction biological sample collection of water bodies.

In order to achieve the above purpose, the magnetic suction type water biological characteristic sample collection system of the preferred embodiment of the present invention includes:

A water body container unit, which is used for accommodating a water body to be sampled;

At least one magnetic suction type biological sample collection device, which is configured in the water body container unit or the water body to be sampled; and

at least one ultrasonic generating device, which is combined with the water body to be sampled;

Wherein, the ultrasonic wave generating device is used to perform ultrasonic vibration treatment on at least one water body to be treated of the sample water body to be collected, so that at least one crushed material of the sample water body to be collected falls into at least one ultrasonically treated water body, and The magnetic suction type biological sample collection device is used to collect and absorb at least one ultrasonically treated water body of the sample water to be collected, so that the magnetic suction type biological sample collection device collects and obtains at least one adsorbed biological sample.

The water body container unit in a preferred embodiment of the present invention is selected from an aquaculture water tank unit, an aquarium display water tank unit, an aquaculture pond unit, an aquarium display pond unit or any combination thereof.

The magnetic suction biological sample collection device in a preferred embodiment of the present invention is selected from a magnetic bead chamber device, a magnetic carrier combined with magnetic beads device, a recirculating fluid body or any combination thereof.

The circulatory fluid body of the preferred embodiment of the present invention has a circulatory fluid area or a circulatory fluid channel.

In a preferred embodiment of the present invention, the magnetic suction type biological sample collection device is connected to the water body container unit through a delivery pipeline.

In a preferred embodiment of the invention the delivery line has a valve device or a switching valve device.

The delivery pipeline in a preferred embodiment of the present invention is combined with a gas delivery device.

In order to achieve the above purpose, the magnetic suction type water biological characteristic sample collection method of the preferred embodiment of the present invention includes:

A magnetic suction type biological sample collection device is arranged in a water body container unit or a water body to be sampled, and the magnetic suction type biological sample collection device is combined with the water body container unit or the water body to be sampled;

combining an ultrasonic generating device with the water body to be sampled so as to transmit an ultrasonic wave to the water body to be sampled;

Ultrasonic vibration treatment using the ultrasonic generating device At least one water body to be treated of the water body to be sampled, so that at least one crushed material of the water body to be sampled falls into at least one water body that has been ultrasonically treated; and

The magnetic suction type biological sample collection device collects and absorbs the ultrasonically treated water body of the sample water to be collected, so that the magnetic suction type biological sample collection device collects and obtains at least one adsorbed biological sample.

In a preferred embodiment of the present invention, the adsorbed biological sample is obtained from at least one adsorbed biological sample magnet, at least one adsorbed biological sample magnetic bead or any combination thereof.

In a preferred embodiment of the present invention, the magnetic suction type biological sample collection device is submerged in the ultrasonically treated water body of the water body container unit.

The magnetic suction type biological sample collection device in a preferred embodiment of the present invention is connected to the water body container unit through a delivery pipeline, so as to transport the ultrasonically treated water to the magnetic suction type biological sample collection device through the delivery pipeline .

The delivery pipeline in a preferred embodiment of the present invention is combined with a gas delivery device, so as to use the gas delivery device to deliver a gas bubble flow to the water body container unit or the water body to be sampled through the delivery pipeline.

1: Water body container unit

10: Water body to be sampled

11: Tissue debris

12: Bubble flow

2: Magnetic suction biological sample collection device

20:Magnetic carrier

20a: Recyclable fluid body

20b: Recirculating fluid zone

201:Magnetic area

21: magnetic beads

22: Gas delivery device

23: Delivery pipeline

3: Ultrasonic generating device

31: Buoyancy device

Figure 1: A schematic diagram of a magnetic suction type water body biological characteristic sample collection system according to the first preferred embodiment of the present invention.

Fig. 2: a flow block diagram of the magnetic suction type water body biometric sample collection method in a preferred embodiment of the present invention.

Figure 3: A schematic diagram of the first magnetic suction biological sample collection device selected for the magnetic suction water body biological characteristic sample collection system in a preferred embodiment of the present invention.

Figure 4: The magnetic suction type water body biological characteristic sample collection system of the preferred embodiment of the present invention chooses to use the second magnetic suction type biological sample collection device intention.

Fig. 5: A schematic diagram of a magnetic suction type water body biometric sample collection system according to the second preferred embodiment of the present invention.

In order to fully understand the present invention, preferred embodiments will be described below in detail together with the accompanying drawings, which are not intended to limit the present invention.

First of all, the magnetic suction type water body biological characteristic sample collection method and its system of the preferred embodiment of the present invention can be applied to various aquatic (or marine) biological restoration industries, various aquatic (or marine) biological seedling breeding industries, various aquatic (or marine) Marine) biological seedling or fish transportation industry, various aquatic (or marine) biological seedling or adult sales business, various aquatic (or marine) biological seedling or adult display business or related aquatic (or marine) biological seedling industry, But it is not intended to limit the scope of application of the present invention.

For example, the magnetic suction type water body biological characteristic sample collection method and its system of the preferred embodiment of the present invention can be applied to the collection of seedlings or adult samples of various aquatic organisms, the collection of seedlings or adult samples of various aquaculture aquatic organisms, various The collection of algae samples in fresh water or marine waters, the collection of various fresh water or seawater cultured algae samples, or the collection of samples in various fresh water or sea waters, but it is not intended to limit the scope of application of the present invention.

Fig. 1 discloses a schematic diagram of a magnetic suction type water body biometric sample collection system according to a first preferred embodiment of the present invention. Please refer to Fig. 1, for example, the magnetic suction type water body biological characteristic sample collection system of the first preferred embodiment of the present invention mainly includes a water body container unit 1, at least one magnetic suction type biological sample collection device 2 and at least one An ultrasonic generating device 3 .

Please refer to Figure 1 again. For example, the water body container unit 1 is used to accommodate a water body 10 to be sampled and several aquatic organisms [not marked], and the water body container unit 1 is selected from a culture water tank unit. , an aquarium display tank unit, an aquaculture tank unit, an aquarium display tank unit or any combination thereof.

Please refer to Fig. 1 again, for example, the magnetic suction type biological sample collection device 2 is arranged in the appropriate position of the water body container unit 1 or the water body 10 to be sampled by appropriate technical means, and the magnetic suction type biological sample The sample collection device 2 is selected from a magnetic bead chamber device, a magnetically attractable carrier combined with a magnetic bead device, a recyclable fluid body or any combination thereof.

Please refer to Fig. 1 again, for example, the ultrasonic generating device 3 is selected to generate a predetermined frequency ultrasonic [for example: 25KHz to 200KHz], and output a predetermined power ultrasonic [for example: continuous or pulsed type ultrasonic wave] to a predetermined time, and the ultrasonic wave generating device 3 is combined with the sample water body 10 to be collected in the water body container unit 1 by appropriate technical means.

Please refer to Figure 1 again. For example, the magnetic suction type biological sample collection device 2 and the ultrasonic wave generating device 3 are selected and assembled by appropriate technical means to form a composite device assembly unit, and the composite device is assembled. The unit can be selected from a single integrated unit, a detachable assembly unit or other various forms of assembly units.

Please refer to Fig. 1 again, for example, when the predetermined frequency ultrasonic wave of the ultrasonic wave generating device 3 can be used to impact the inner wall material of the water body container unit 1 (not shown), the sample water body 10 to be collected Contains impurities (not shown) and surface germs (not shown) of aquatic organisms, parasites (not shown) or tissue debris (tissue) 11, and can clean the inner wall surface of the water body container unit 1 at the same time.

FIG. 2 discloses a flow block diagram of a magnetic suction type water body biometric sample collection method in a preferred embodiment of the present invention, which is correspondingly applicable to the magnetic suction type water body biometric sample collection system in FIG. 1 . Please refer to Figures 1 and 2, the magnetic suction type water body biometric sample collection method in a preferred embodiment of the present invention includes step S1: first, for example, with appropriate technical means [for example: automatic mode, semi-automatic mode or Manual mode] The magnetic suction type biological sample collection device 2 is arranged on the water body container unit 1 or its Collect the sample water body 10, and combine the magnetic biological sample collection device 2 with the water body container unit 1 or the sample water body 10 to be collected by appropriate technical means (for example: fixed or movable).

Please refer to Figures 1 and 2 again, the magnetic suction type water biometric sample collection method in a preferred embodiment of the present invention includes step S2: using appropriate technical means (for example: automatic, semi-automatic or manual) The ultrasonic generating device 3 is combined with the sample water body 10 of the water body container unit 1 to properly transmit an ultrasonic wave to the sample water body 10 of the water body container unit 1 .

Please refer to Figures 1 and 2 again, the magnetic suction type water body biometric sample collection method of the preferred embodiment of the present invention includes step S3: then, for example, with appropriate technical means [such as: automatic mode, semi-automatic mode or manual mode] utilize the ultrasonic generating device 3 to carry out ultrasonic vibration treatment of at least one water body to be treated in the sample water body 10 to be collected, so as to drop at least one crushed material of the sample water body 10 into at least one Ultrasonic treatment of water in the body.

Please refer to Figures 1 and 2 again, the magnetic suction type water body biometric sample collection method of the preferred embodiment of the present invention includes step S4: then, for example, with appropriate technical means [for example: automatic mode, semi-automatic mode or manual mode] utilize the magnetic suction type biological sample collection device 2 to collect, absorb and process the ultrasonically treated water body of the sample water body 10 of the water body container unit 1, so that the magnetic suction type biological sample collection device 2 collects and obtains at least - Adsorbed biological samples.

Please refer to FIG. 1 and FIG. 2 again. For example, the adsorbed biological sample is obtained from at least one adsorbed biological sample magnet, at least one adsorbed biological sample magnetic bead or any combination thereof. In addition, the magnetic suction type biological sample collection device 2 can optionally be submerged in the ultrasonically treated water body of the water body container unit 1 .

Please refer to Figures 1 and 2 again, for example, the magnetic attraction The type biological sample collection device 2 can be selected to sink into the sample water body 10 to be collected or the water body treated with ultrasonic waves to a predetermined sampling depth and a predetermined sampling position; similarly, the ultrasonic wave generating device 3. It can be selected to properly sink into the water body 10 to be sampled or the water body treated with ultrasonic waves arranged in the water body container unit 1 to a predetermined sampling depth and a predetermined sampling position.

Please refer to Figures 1 and 2 again. For example, the biological sample adsorbed by the magnetic suction type biological sample collection device 2 includes the water body container unit produced by the ultrasonic shock or vibration treatment performed by the ultrasonic wave generating device 3. 1 Inner wall surface material, impurities contained in the water body 10 to be collected, and surface bacteria, parasites or tissue debris 11 of aquatic organisms.

Figure 3 shows a schematic diagram of the first magnetic biological sample collection device selected for the magnetic suction water biological sample collection system in a preferred embodiment of the present invention, which corresponds to the magnetic suction biological sample collection device in Figure 1 . Please refer to Figure 3, for example, the magnetic suction type water body biological characteristic sample collection system of the preferred embodiment of the present invention selects a first magnetic suction type biological sample collection device, and the first magnetic suction type biological sample The collection device includes at least one or several magnetically attractable carriers [magnetically attractable carrier] 20 and several magnetic beads [magnetic beads] 21, and optionally includes a magnetic bead removal device [not shown], a sample dissolution device [ not shown] or a device with a similar function.

Please refer to Fig. 3 again, for example, the magnetically attractable carrier 20 has at least one or several magnetically attractable regions 201, and at least one or severally magnetically attractable regions 201 correspond to several of the magnetic beads twenty one. The magnetically attractable carrier 20 in another preferred embodiment of the present invention is selected from a rod, a piece, a sphere, an ellipsoid (rugby ball), a block, an object of any geometric shape or any combination thereof.

Please refer to FIG. 3 again, for example, the magnetic beads 21 can be properly adsorbed and disposed on the magnetically attractable region 201 of the magnetically attractable carrier 20 . Other In addition, the magnetic bead 21 is selected to be made of an appropriate material (for example: ferric oxide or other magnetic materials), and the magnetic bead 21 is selected to have a diameter of an appropriate size, and the magnetic bead 21 has an appropriate appearance shape, for example: irregular surface.

Please refer to Fig. 3 again. For example, the magnetic bead 21 (shown in the schematic frame of Fig. 3 ) can be selected from a magnetic bead with a chemically modified surface, and the chemically modified surface of the magnetic bead 21 is properly selected. It has various functional groups such as amine, carboxyl or hydroxyl. In this way, the surface of the magnetic beads 21 can adsorb different microorganisms or biomolecules, but this is not intended to limit the scope of application of the present invention.

Figure 4 discloses a schematic diagram of the second magnetic suction biological sample collection device selected for the magnetic suction water biological sample collection system of a preferred embodiment of the present invention, which corresponds to the first magnetic suction biological sample collection in Figure 3 device. Please refer to Figure 4, for example, the magnetic suction type water body biological characteristic sample collection system of the preferred embodiment of the present invention selects a second magnetic suction type biological sample collection device, and the second magnetic suction type biological sample The collection device includes at least one or several recirculating fluid bodies [fluid-circulating body] 20a and several magnetic beads [magnetic beads] 21, and optionally includes a magnetic bead removal device [not shown], a sample dissolution device [not shown] or a device with a similar function.

Please refer to Fig. 4 again, for example, the recirculating fluid body 20a has at least one or several recirculating fluid regions 20b or at least one or several recirculating fluid passages [fluid passage], and at least one or several A plurality of the recirculating fluid regions 20b or recirculating fluid channels corresponds to a plurality of the magnetic beads 21 .

Please refer to Fig. 4 again, for example, the at least one or several recirculating fluid regions 20b or at least one or several recirculating fluid passages can be selectively arranged on the outer surface and side end surface of the recirculating fluid body 20a , inner wall surface, interior or other appropriate locations, but it is not intended to limit the scope of the present invention.

Please refer to Fig. 4 again, for example, the recirculating fluid body 20a of another preferred embodiment of the present invention is selected from a bottle, a box, a pipe, a cavity, a sphere, a An ellipsoid (rugby ball), a floating sphere, a buoy, a container body that can be placed in water (for example: a closed or semi-open container body) or any combination thereof.

Please refer to Fig. 4 again, for example, the recirculating fluid area 20b or the recirculating fluid channel of the recirculating fluid body 20a can be configured with at least one or several magnetically attractive areas or similar magnetically attractive areas. The functional device is used to magnetically adsorb several magnetic beads 21 arbitrarily or regularly, but it is not intended to limit the scope of the present invention.

Please refer to Fig. 4 again, for example, the recirculating fluid body 20a can be selected from a buoyant recirculating fluid body, a movable recirculating fluid body or a fixed recirculating fluid body in the system setting , but it is not intended to limit the scope of the present invention.

Please refer to Fig. 4 again, for example, in structure, this recirculating fluid body 20a can choose to configure at least one or several chambers [chamber], at least one or several fluid inlets [fluid inlet] or fluid An input pipe and at least one or several fluid outlets (fluid outlet) or fluid output pipes, but it is not intended to limit the scope of the present invention.

Fig. 5 discloses a schematic diagram of a magnetic suction type water body biometric sample collection system according to a second preferred embodiment of the present invention. Please refer to Fig. 5, compared with the first embodiment, the magnetic suction type water body biometric sample collection system of the second preferred embodiment of the present invention further includes a gas delivery device 22 and a delivery pipeline 23, and the delivery The pipeline 23 is combined with the gas delivery device 22, so that the gas delivery device 22 is used to deliver a bubble flow 12 to the water body container unit 1 or the sample water body to be collected through the delivery pipeline 23, so that in the water body container unit 1 to be collected A circulating water flow is generated in the sample water body, and the circulating water flow is guided through the interior of the magnetic suction type biological sample collection device 2 . In addition, the delivery pipeline 23 has a valve device (not shown) or a Switching valve device [not shown].

Please refer to shown in Fig. 5 again, for example, this ultrasonic generation device 3 has a buoyancy device 31 or other device that has provided buoyancy, and this buoyancy device 31 is combined with this ultrasonic generation device 3 by appropriate technical means, In order to arrange the ultrasonic wave generating device 3 in the water body container unit 1 or the water body to be sampled in a floating manner.

The above-mentioned preferred embodiments only illustrate the present invention and its technical characteristics, and the technology of this embodiment can still be implemented in various substantially equivalent modifications and/or replacements; therefore, the scope of rights of the present invention depends on the appended patent application The scope defined by the scope shall prevail. The copyright in this case is restricted to be used for patent applications in the Republic of China.

1: Water body container unit

10: Water body to be sampled

11: Tissue debris

2: Magnetic suction biological sample collection device

3: Ultrasonic generating device

Claims (10)

A magnetic suction type water body biological characteristic sample collection system, which includes: a water body container unit, which is used to accommodate a water body to be collected; at least one magnetic suction type biological sample collection device, which is configured in the water body container unit or to be collected A sample water body; and at least one ultrasonic generating device, which is combined with the sample water body to be collected; wherein the ultrasonic wave generating device is used to perform ultrasonic vibration treatment on at least one of the sample water body to be processed, so that the sample to be collected At least one crushed substance of the water body falls into at least one ultrasonically treated water body, and the magnetic suction type biological sample collection device is used to collect and absorb at least one ultrasonically treated water body of the sample water body to be collected, so as to facilitate the magnetic The suction biological sample collection device collects and obtains at least one adsorbed biological sample, and the adsorbed biological sample includes the wall surface material of the water body container unit, impurities contained in the sample water to be collected, and surface bacteria, parasites or tissue fragments of aquatic organisms. crumbs. According to the magnetic suction type water biological sample collection system described in item 1 of the scope of patent application, wherein the magnetic suction type biological sample collection device is selected from a magnetic bead chamber device, a magnetically attractable carrier combined with a magnetic bead device, a Circulating fluid body or any combination thereof. According to the magnetic suction type water body biological sample collection system described in item 1 of the scope of the patent application, the magnetic suction type biological sample collection device is connected to the water body container unit through a delivery pipeline. According to the magnetic suction type water body biometric sample collection system described in item 3 of the scope of the patent application, the delivery pipeline has a valve device or a switch valve device. According to the magnetic suction water body biometric sample collection system described in item 3 of the scope of the patent application, the delivery pipeline is combined with a gas delivery device. A magnetic suction type biological sample collection method of water body, which comprises: a magnetic suction type biological sample collection device is arranged in a water body container unit or a water body to be sampled, and the magnetic suction type biological sample collection device is combined fit to the water body container unit or the water body to be sampled; combine an ultrasonic generating device with the sample water body to be collected so as to transmit an ultrasonic wave to the sample water body to be collected; use the ultrasonic generating device to perform ultrasonic vibration treatment At least one water body to be treated of the sample water body to be collected, so that at least one crushed substance of the sample water body to be collected falls into at least one ultrasonically treated water body; The water body of the water body to be sampled has been treated with ultrasonic waves, so that the magnetic suction type biological sample collection device can collect at least one adsorbed biological sample, and the adsorbed biological sample includes the inner wall material of the water body container unit, the water body of the sampled water body to be collected Body surface germs, parasites or tissue debris containing impurities and aquatic organisms. According to the magnetic suction water biological sample collection method described in item 6 of the patent application, the adsorbed biological sample is taken from at least one adsorbed biological sample magnet, at least one adsorbed biological sample magnetic bead or any combination thereof. According to the magnetic suction type water body biological sample collection method described in item 6 of the scope of the patent application, the magnetic suction type biological sample collection device is submerged in the ultrasonically treated water body of the water body container unit. According to the magnetic suction type water body biological sample collection method described in item 6 of the scope of the patent application, wherein the magnetic suction type biological sample collection device is connected to the water body container unit through a delivery pipeline, so that the ultrasonically treated water body can be passed through The delivery pipeline is delivered to the magnetic suction type biological sample collection device. According to the magnetic suction type water body biometric sample collection method described in item 9 of the scope of the patent application, wherein the delivery pipeline is combined with a gas delivery device, so as to use the gas delivery device to deliver an air bubble flow to the water body container through the delivery pipeline Unit or water body to be sampled.

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