TWI728559B - Circulating type microorganism or biomolecule collecting system and method thereof for environment - Google Patents

Abstract

A circulating type microorganism or biomolecule collecting method includes: providing a plurality of magnetic beads in a fluid-circulating body to form a circulating bead collector body; arranging the fluid-circulating body and magnetic beads thereof in an environment to fluid-circularly collect a plurality of microorganism or biomolecule samples to form a plurality of microorganism or biomolecule-binding beads; releasing or drawing out the plurality of microorganism or biomolecule-binding beads from the fluid-circulating body; and extracting the plurality of microorganism or biomolecule samples from the plurality of microorganism or biomolecule-binding beads.

Description

Microbe or biomolecule collection system and method in circulating environment

The present invention relates to a circulating type environment (environment) microorganism (microorganism) or biomolecule (biomolecule) collection system and method thereof; in particular, it relates to a portable (portable) circulating environment of microorganisms or organisms Molecular collection system and method.

For example, the conventional non-circulating magnetic bead type specimen separation technology and its device, such as the invention patent of "magnetic bead type specimen separation device" of the Republic of China Patent Publication No. TW-I296713, which discloses a magnetic bead type specimen separation device体分器。 Body separation device. The magnetic bead sample separation device includes a body, a first reaction tank, a second reaction tank, a third reaction tank, a first micro flow channel and a second micro flow channel. The first reaction tank, the second reaction tank, the third reaction tank, the first micro flow channel and the second micro flow channel are arranged in the body.

In addition, the aforementioned No. TW-I296713 injects a mixed solution into the first reaction tank, and the mixed solution includes a plurality of magnetic beads and a sample extract, and the sample extract is bound to the magnetic beads. Inject a washing buffer [washing buffer] into the second reaction tank, and inject a separation buffer [elution buffer] into the third reaction tank and the third reaction tank, and the second reaction tank is located in the first reaction tank and Between the third reaction tank. The first micro flow channel is connected to the first reaction tank and the second reaction tank, and the second micro flow channel is connected to the second reaction tank and the third reaction tank.

Continuing, the bead of the aforementioned No. TW-I296713 consists of a magnetic The force drives the movement from the first reaction tank to the second reaction tank via the first micro flow channel, so that the washing buffer can clean the magnetic beads. The magnetic beads are then driven by the magnetic force to move from the second reaction tank to the third reaction tank via the second microfluidic channel, so that the separation buffer can separate the magnetic beads and the sample extract.

However, the structure of the main body, the first reaction tank, the second reaction tank, the third reaction tank, the first microfluidic channel and the second microfluidic channel of the magnetic bead sample separation device of No. TW-I296713 mentioned above is only simple It is only suitable for general non-circulating magnetic bead sample separation technology, so it is not suitable for the collection technology of microorganisms or biomolecules in a circulating environment.

Another conventional non-circulating biomolecule experiment with test-tube type micro-magnetic bead immobilization technology and its device, for example: the new patent of "test-tube type micro-magnetic bead immobilization device" of Republic of China Patent Publication No. TW-M536587, which discloses a test-tube type Micro-magnetic bead fixing device. The test tube type micro-magnetic bead fixing device includes an upper seat, a lower seat, a middle column, a ring magnet and two rings.

On the other hand, the upper base of No. TW-M536587 is a disc shape, and the outer ring of the upper base is provided with a plurality of fixing holes, and the fixing holes penetrate the upper base, and the fixing holes can be inserted separately A test tube, and an upper seat groove is provided on the bottom surface of the upper seat.

In addition, the lower seat of No. TW-M536587 mentioned above is in the shape of a disc, and the outer ring of the lower seat is provided with a plurality of fixing grooves, and the fixing hole corresponds to the fixing groove, and a plurality of the fixing grooves are available for The test tubes after being inserted are pressed against each other, and the top surface of the lower seat is provided with a lower seat groove, and the upper seat groove corresponds to the lower seat groove.

In addition, the center column of No. TW-M536587 is set between the upper seat and the lower seat, and the upper end surface and the lower end surface of the center column abut against the groove of the upper seat and the groove of the lower seat, respectively. The ring magnet is sleeved on the center column, and the two loops are sleeved on the center column, and are respectively located between the upper seat and the ring magnet, and between the lower seat and the ring magnet.

However, the above-mentioned structure of the test-tube type micro-magnetic bead fixing device of No. TW-M536587 above, the lower base, the middle column, the ring magnet and the ring is only suitable for the general non-circulating biomolecule experiment. The bead fixation technology is only, so it is not suitable for the collection technology of microorganisms or biomolecules in the environment.

Another conventional non-circulating magnetic bead sample nucleic acid extraction technology and its equipment, such as the new patent of "Magnetic Bead Nucleic Acid Extraction Equipment" of the Republic of China Patent Publication No. TW-M445583, which discloses a magnetic bead type nucleic acid extraction equipment . The magnetic bead nucleic acid extraction equipment includes a first test tube cassette, a magnetic bead sample purification device, a detection test tube, and a detection device.

In addition, the first test tube cassette of the aforementioned No. TW-M445583 has a plurality of test tubes, and the test tubes are arranged adjacently, and the test tube is used for accommodating a plurality of magnetic beads and at least one specimen. The magnetic bead type specimen purification device is used to perform a separation process and an extraction process on the specimen. The magnetic bead type sample purification device includes a frame and a magnetic control device, and the frame carries the first test tube cassette, and the magnetic control device provides a magnetic force to drive the magnetic beads to move for the extraction process to obtain One extract.

In addition, the aforementioned detection test tube No. TW-M445583 is used to contain the extract, and the detection device includes an accommodating space, an ultraviolet light source and a sensing unit, and the accommodating space accommodates the extract. Detection test tube. The ultraviolet light source outputs a light beam, and the light beam has a plurality of different wavelengths of ultraviolet light, and the light beam is projected to the detection test tube, and the sensing unit is arranged on the traveling path of the light beam to receive and pass the detection The beam of the extract in the test tube.

However, the structure of the first test tube cassette, magnetic bead sample purification device, detection test tube, and detection device of the magnetic bead nucleic acid extraction equipment of No. TW-M445583 mentioned above is only suitable for general non-circulating biomolecular experiments. The test tube type micro-magnetic bead fixation technology is only used, so it is not suitable for the collection technology of microorganisms or biomolecules in the environment.

Obviously, the aforementioned magnetic bead sample separation device of No. TW-I296713, the test tube type micro-magnetic bead fixing device of No. TW-M536587, and the magnetic bead-type nucleic acid extraction device of No. TW-M445583 must have further requirements. The potential demand for improving its overall technology.

The aforementioned inventions or new patents of Republic of China Patent No. TW-I296713, Announcement No. TW-M536587 and Announcement No. TW-M536587 are only a reference for the technical background of the present invention and illustrate the current state of technological development, and they are not intended to limit The scope of the invention.

In view of this, in order to meet the above needs, the present invention provides a system and method for collecting microorganisms or biomolecules in a circulating environment, which utilizes a circulated fluid body to configure several magnetic beads to form a magnetic bead circulating collection body , And place the circulated fluid body and its magnetic beads in an environment to collect several samples of microorganisms or biomolecules in a circulating flow manner to form several microorganisms or biomolecules adsorbed magnetic beads, and the plurality of microorganisms or biomolecules The adsorbed magnetic beads are taken out of the recirculating fluid body, and several microbes or biomolecule samples are subsequently taken out from several microbes or biomolecules adsorbed magnetic beads, so as to improve the technical problems of microbes or biomolecule collection in the conventional environment.

The main purpose of the preferred embodiments of the present invention is to provide a system and method for collecting microorganisms or biomolecules in a circulating environment, which utilizes a circulated fluid body to configure several magnetic beads to form a magnetic bead circulating collecting body, and The circulated fluid body and its magnetic beads are placed in an environment to collect samples of microorganisms or biomolecules in a circulating flow mode to form a plurality of microorganisms or biomolecules adsorbed magnetic beads, and the microorganisms or biomolecules are adsorbed magnetically. Beads are taken out from the circulated fluid body, and several microbes or biomolecule samples are subsequently taken out from several microbes or biomolecule adsorption magnetic beads, so as to achieve the purpose of recycling microbes or biomolecules in the environment.

In order to achieve the above objectives, the collection system for microorganisms or biomolecules in a circulating environment in a preferred embodiment of the present invention includes:

At least one circulated fluid body, which has a circulated fluid area or a circulated fluid channel; and

A plurality of first magnetic beads, which are arranged in the circulating fluid area or the circulating fluid channel of the circulating fluid body to form a first magnetic bead circulating collecting body;

The circulated fluid body and its first magnetic beads are placed in an environment to collect a number of microbes or biomolecule samples in a circulating flow manner to form a number of first microbes or biomolecules adsorbed magnetic beads, and a number of the The first microbe or biomolecule adsorption magnetic beads are taken out from the circulated fluid body, and a plurality of the microbe or biomolecule samples are subsequently taken out from a plurality of the first microbes or biomolecule adsorption magnetic beads.

In another preferred embodiment of the present invention, the system for collecting microorganisms or biomolecules in a circulating environment includes:

At least one circulated fluid body, which has a first circulated fluid zone or a first circulated fluid channel and a second circulated fluid zone or a second circulated fluid channel;

A plurality of first magnetic beads, which are arranged in the first circulated fluid area or the first circulated fluid channel of the circulated fluid body; and

A plurality of second magnetic beads are arranged in the second circulated fluid area or the second circulated fluid channel of the circulated fluid body, so that a first magnetic bead and a second magnetic bead are formed by the first magnetic bead and the second magnetic bead Two magnetic beads circulating collection body;

The circulated fluid body, its first magnetic bead and its second magnetic bead are placed in an environment to collect several samples of microorganisms or biomolecules in a circulating flow manner to form a plurality of first microorganisms or biomolecule adsorption magnetic beads And a plurality of second microorganisms or biomolecules to adsorb the magnetic beads, and a plurality of the first microorganisms or biomolecules to adsorb the magnetic beads and a plurality of the second microorganisms or biological molecules The sub-adsorbed magnetic beads are taken out from the circulated fluid body, and several samples of the microorganisms or biomolecules are subsequently taken out from a plurality of the first microorganisms or biomolecules adsorbed magnetic beads and a plurality of the second microorganisms or biomolecules adsorbed magnetic beads .

The circulated fluid body of the preferred embodiment of the present invention is selected from a bottle body, a box body, a tube body, a cavity, a sphere, an ellipsoidal sphere, a floating sphere, a buoy body or any combination thereof.

The circulated fluid body of the preferred embodiment of the present invention has at least one or several fluid inlets and at least one or several fluid discharge ports.

In a preferred embodiment of the present invention, the first magnetic bead or the second magnetic bead is selected from a magnetic bead with a chemically modified surface.

The surface of the first magnetic bead or the second magnetic bead in the preferred embodiment of the present invention can adsorb different microorganisms or biological molecules.

In order to achieve the above objective, the method for collecting microorganisms or biomolecules in a circulating environment in a preferred embodiment of the present invention includes:

A circulated fluid body is used to configure a plurality of first magnetic beads to form a first magnetic bead circulating collection body;

Placing the circulated fluid body and its first magnetic beads in an environment to collect a plurality of microorganisms or biomolecule samples in a circulating flow manner to form a plurality of first microorganisms or biomolecule adsorption magnetic beads;

Taking out a plurality of the first microbe or biomolecule adsorption magnetic beads from the recirculating fluid body; and

A plurality of samples of the microorganisms or biomolecules are subsequently taken out from a plurality of the first microorganisms or biomolecule adsorption magnetic beads.

The method for collecting microorganisms or biomolecules in a circulating environment according to another preferred embodiment of the present invention includes:

A circulated fluid body is used to configure a plurality of first magnetic beads and a plurality of second magnetic beads, so that the first magnetic beads and the second magnetic beads form a first magnetic bead and a second magnetic bead circulating collection body;

The circulated fluid body, its first magnetic beads and its second magnetic beads are placed in an environment to collect samples of microorganisms or biomolecules in a circulating flow mode to form a plurality of first microorganisms or biomolecule adsorption magnetic beads and Several second microbes or biomolecules adsorbed magnetic beads;

Taking out a plurality of the first microbe or biomolecule adsorption magnetic beads and a plurality of the second microbe or biomolecule adsorption magnetic beads from the circulated fluid body; and

A plurality of samples of the microorganisms or biomolecules are subsequently taken out from a plurality of the first microorganisms or biomolecules adsorbed magnetic beads and a plurality of the second microorganisms or biomolecules adsorbed magnetic beads.

The circulated fluid body of the preferred embodiment of the present invention is selected from a bottle body, a box body, a tube body, a cavity, a sphere, an ellipsoidal sphere, a floating sphere, a buoy body or any combination thereof.

The circulated fluid body of the preferred embodiment of the present invention has at least one or several fluid inlets and at least one or several fluid discharge ports.

In a preferred embodiment of the present invention, the first magnetic bead or the second magnetic bead is selected from a magnetic bead with a chemically modified surface.

The surface of the first magnetic bead or the second magnetic bead in the preferred embodiment of the present invention can adsorb different microorganisms or biological molecules.

1‧‧‧Circulating fluid body

10‧‧‧Circulating fluid area

1a‧‧‧Circulating fluid body

11‧‧‧The first circulating fluid zone

12‧‧‧Second Circulating Fluid Zone

2‧‧‧Magnetic beads

2a‧‧‧The first magnetic bead

2b‧‧‧Second magnetic bead

3‧‧‧DNA

a‧‧‧Fluid inlet

b‧‧‧Fluid discharge port

Figure 1: A schematic diagram of a collection system for microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention.

Figure 2: The flow block diagram of the method for collecting microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention.

Figure 3: A schematic diagram of a collection system for microorganisms or biomolecules in a circulating environment according to a second preferred embodiment of the present invention.

Figure 4: Microorganisms in a circulating environment according to the second preferred embodiment of the present invention Or the flow block diagram of the biomolecule collection method.

In order to fully understand the present invention, preferred embodiments are exemplified below and described in detail with the accompanying drawings, and they are not intended to limit the present invention.

First of all, the collection system and method for microorganisms or biomolecules in a circulating environment of the preferred embodiment of the present invention can be applied to various waters (for example: freshwater waters, estuarine waters, intertidal or ocean waters) or microorganisms or biomolecules in ecosystems Collection operations or water quality inspection operations, various aquatic (or marine) biological seedling breeding industries or related aquatic (or marine) biological seedling industries, but they are not intended to limit the scope of application of the present invention.

For example, the collection system and method for microorganisms or biomolecules in a circulating environment in a preferred embodiment of the present invention adopts the technical term "microorganism" to define viruses, bacteria, fungus, or other microorganisms; The collection system and method for microorganisms or biomolecules in a circulating environment in a preferred embodiment of the present invention uses the technical term "biomolecule" to define DNA, RNA, protein or other biological molecules, such as biological macromolecules, but it is not used for Limit the scope of application of the present invention.

Fig. 1 shows a schematic diagram of a collection system for microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention. Please refer to Figure 1. For example, the microbial or biomolecule collection system in the circulating environment of the first preferred embodiment of the present invention includes at least one or several fluid-circulating bodies (fluid-circulating bodies) 1 and Several magnetic beads 2 to form a portable collection system for microorganisms or biomolecules, and optionally include a magnetic bead removal device (not shown), a sample dissolution device (not shown), or a Devices with similar functions.

Please refer to Figure 1 again. For example, the circulated fluid body 1 has at least one or more circulated fluid regions 10 or at least one or more circulated fluid passages (fluid passages), and at least one or more Each of the circulated fluid areas 10 or circulated fluid channels corresponds to a number of the magnetic beads 2.

Please refer to Figure 1 again. For example, the at least one or more circulated fluid areas 10 or at least one or more circulated fluid channels can be optionally configured on the outer surface and side end surfaces of the circulated fluid body 1 , The inner wall surface, the interior or other suitable positions, but it is not used to limit the scope of the present invention.

Please refer to Figure 1 again. For example, the circulated fluid body 1 of another preferred embodiment of the present invention is selected from a bottle body, a box body, a tube body, a cavity, a sphere, a Elliptical sphere (football), a floating sphere, a buoy body, a container body that can be placed in the water (for example, a closed or semi-open container body) or any combination thereof.

Please refer to Figure 1 again. For example, in terms of the structure, the circulatory fluid area 10 or the circulatory fluid channel of the circulatory fluid body 1 can optionally be configured with at least one or several magnetically attractable areas or have similar magnetic attraction. It is a functional device to magnetically adsorb several magnetic beads 2 randomly or regularly, but it is not intended to limit the scope of the present invention.

Please refer to Figure 1 again. For example, the circulated fluid body 1 can be selected from a floatable circulatory fluid body, a movable circulatory fluid body or a stationary circulatory fluid body on the system setup. , But it is not used to limit the scope of the present invention.

Please refer to Figure 1 again. For example, the circulated fluid body 1 can be configured with at least one or more chambers, at least one or more fluid inlets, or fluid inlets. The input pipe and at least one or several fluid outlets (fluid outlets) or fluid output pipes are not used to limit the scope of the present invention.

Please refer to FIG. 1 again. For example, the magnetic beads 2 can be appropriately adsorbed and arranged in the circulated fluid area 10 or the circulated fluid channel of the circulated fluid body 1. In addition, the magnetic beads 2 are selected from appropriate materials (for example: It is made of ferroferric oxide or other magnetic materials], and the magnetic bead 2 is selected to have a diameter of an appropriate size and size, and the magnetic bead 2 has an appropriate appearance shape, such as an irregular surface.

Please refer to Figure 1 again. For example, the magnetic bead 2 (shown in the schematic frame of Figure 1) can be selected from a magnetic bead with a chemically modified surface, and the chemically modified surface of the magnetic bead 2 is appropriately selected It has various functional groups, such as amino, carboxyl or hydroxyl. In this way, the surface of the magnetic beads 2 can adsorb different microorganisms or biomolecules, but it is not used to limit the scope of application of the present invention.

Fig. 2 shows a flow block diagram of the method for collecting microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention, which corresponds to the microorganisms or biomolecules collecting system in a circulating environment of Fig. 1. Please refer to Figures 1 and 2, the method for collecting microorganisms or biomolecules in the environment of the first preferred embodiment of the present invention includes step S1: First, for example, by appropriate technical means (such as direct implantation or magnetic Suction method] A number of the magnetic beads 2 are arranged by using the circulating fluid area 10 or the circulating fluid channel of the circulating fluid body 1 to form a magnetic bead circulating collecting body (that is, the magnetic beads are equipped with a chamber).

Please refer to Figures 1 and 2, the method for collecting microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention includes step S2: Then, for example, the circulating fluid body 1 and its magnetic The beads 2 are placed in an environment (such as a microbe or biomolecule sampling operation environment) by appropriate technical means to collect several microbes or biomolecule samples in a circulating flow mode to form several microbes or biomolecule adsorption magnetic beads.

Please refer to Figures 1 and 2, the method for collecting microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention includes step S3: Then, for example, a plurality of the microorganisms or biomolecules are adsorbed magnetically. Beads can be circulated from the circulated fluid body 1 by appropriate technical means (for example: magnetic operation with a magnetic base removal device or a magnetic ring removal device) The fluid 10 can be released or drawn out from the circulating fluid channel.

Please refer to Figures 1 and 2 again. The method for collecting microorganisms or biomolecules in a circulating environment according to the first preferred embodiment of the present invention includes step S4: Then, for example, by appropriate technical means (e.g., using a lysis solution) , Rinse solution and return solution method] A plurality of samples of the microorganisms or biomolecules are subsequently taken out from a plurality of the first microorganisms or biomolecules adsorbed magnetic beads.

Please refer to Figures 1 and 2, the method for collecting microorganisms or biomolecules in a circulating environment in the first preferred embodiment of the present invention can choose to use a magnetic bead removal tube, a magnetic bead removal bucket, and a magnetic bead. The unloading chamber or any combination thereof can be selected from an automatic device, a semi-automatic device or a manual device.

Fig. 3 shows a schematic diagram of a collection system for microorganisms or biomolecules in a circulating environment according to a second preferred embodiment of the present invention. Please refer to Fig. 3, for example, compared to the first embodiment, the microorganism or biomolecule collection system in the circulating environment of the second preferred embodiment of the present invention includes a recirculating fluid body 1a and a plurality of first The magnetic beads 2a and several second magnetic beads 2b form a portable microorganism or biomolecule collection system.

Please refer to Fig. 3 again. For example, the circulated fluid body 1a has at least one or more first circulated fluid areas 11 or first circulatory fluid channels and at least one or more second circulatory fluids. Zone 12 or the second circulated fluid channel, and at least one or several of the first circulated fluid zone 11 or first circulated fluid channel corresponds to a plurality of the first magnetic beads 2a, and at least one or several of the first magnetic beads 2a The two circulated fluid areas 12 or the second circulated fluid channels correspond to a plurality of the second magnetic beads 2b.

Please refer to Fig. 3 again. For example, the first circulated fluid zone 11 or the first circulated fluid channel and the second circulated fluid zone 12 or the second circulated fluid zone of the circulated fluid body 1 are structurally The fluid channel can be equipped with at least one or several magnetically attractable areas or a device with similar magnetic attraction function. It is arranged so as to magnetically adsorb several magnetic beads 2 randomly or regularly, but it is not used to limit the scope of the present invention.

Please refer to Figure 3 again. For example, in another preferred embodiment of the present invention, the surfaces of the first magnetic beads 2a and the second magnetic beads 2b can be selected to adsorb different microorganisms or biological molecules to form a A collection system for microorganisms or biomolecules in a compound circulating environment.

FIG. 4 shows a flow block diagram of the method for collecting microorganisms or biomolecules in a circulating environment according to the second preferred embodiment of the present invention, which corresponds to the microorganisms or biomolecules collecting system in a circulating environment in FIG. 3. Please refer to Figures 3 and 4, the method for collecting microorganisms or biomolecules in a circulating environment according to the second preferred embodiment of the present invention includes step S1A: First, for example, the recyclable fluid body 1a is utilized by appropriate technical means. The first circulating fluid zone 11 or the circulating fluid channel and the second circulating fluid zone 12 or the circulating fluid channel are configured with a plurality of the first magnetic beads 2a and a plurality of the second magnetic beads 2b, so that the The magnetic beads 2a and the second magnetic beads 2b form a first magnetic bead and a second magnetic bead circulating collecting body, that is, a composite magnetic bead circulating collecting body.

Please refer to Figures 3 and 4 again. The method for collecting microorganisms or biomolecules in a circulating environment according to the second preferred embodiment of the present invention includes step S2A: Then, for example, the recirculating fluid body 1a and the second preferred embodiment of the method include step S2A. A magnetic bead 2a and its second magnetic bead 2b are placed in an environment by appropriate technical means to collect several samples of microorganisms or biomolecules in a circulating flow manner to form a plurality of first microorganisms or biomolecule adsorption magnetic beads and a plurality of second magnetic beads. 2. Microbes or biomolecules adsorb magnetic beads.

Please refer to Figures 3 and 4 again. The method for collecting microorganisms or biomolecules in a circulating environment according to the second preferred embodiment of the present invention includes step S3A: Then, for example, a plurality of the first microorganisms or biomolecules The magnetic adsorption beads and a plurality of the second microbe or biomolecule adsorption magnetic beads are removed from the first circulating fluid zone 11 of the circulating fluid body 1a by appropriate technical means. Or the recirculating fluid channel and the second recirculating fluid zone 12 or the recirculating fluid channel are unloaded or taken out.

Please refer to Figures 3 and 4 again. The method for collecting microorganisms or biomolecules in a circulating environment according to the second preferred embodiment of the present invention includes step S4A: Then, for example, use appropriate technical means to collect the first Microbes or biomolecules adsorbed magnetic beads and a plurality of the second microbes or biomolecules adsorbed magnetic beads are subsequently taken out of a plurality of samples of the microorganisms or biomolecules.

Please refer to Figure 3 again. For example, the circulated fluid body 1a of another preferred embodiment of the present invention has a first side and a second side, and the first side of the circulated fluid body 1a The side is optionally configured as a fluid inlet a, and the second side of the circulated fluid body 1a is optionally configured as a fluid discharge port b, and the circulatory fluid body 1a can optionally be configured with at least one fluid drive element (for example: underwater Motor or other fluid drive], but it is not intended to limit the scope of application of the present invention.

The foregoing preferred embodiments only illustrate the present invention and its technical features. The technology of this embodiment can still be implemented with various substantially equivalent modifications and/or alternatives; therefore, the scope of rights of the present invention is subject to a patent application. The scope defined by the scope shall prevail. The copyright in this case is restricted to the use of patent applications in the Republic of China.

1‧‧‧Magnetic-attractable carrier

10‧‧‧Circulating fluid area

2‧‧‧Magnetic beads

3‧‧‧DNA

Claims (10)

A collection system for microorganisms or biomolecules in a circulating environment, which comprises: at least one circulatory fluid body having a circulatory fluid area or a circulatory fluid channel; and a plurality of first magnetic beads arranged in the circulatory fluid body The circulating fluid area or the circulating fluid channel of the fluid body forms a first magnetic bead circulating collection body; wherein the circulating fluid body and the first magnetic beads are placed in an environment to collect a number of them in a circulating flow manner A sample of microorganisms or biomolecules to form a plurality of first microorganisms or biomolecule adsorption magnetic beads, and a plurality of the first microorganisms or biomolecule adsorption magnetic beads are taken out of the recirculating fluid body, and from a plurality of the first microorganisms Or biomolecules adsorb magnetic beads to subsequently take out several samples of the microorganisms or biomolecules. A collection system for microorganisms or biomolecules in a circulating environment, comprising: at least one circulated fluid body having a first circulated fluid zone or a first circulated fluid channel and a second circulated fluid zone or a first Two circulated fluid channels; a plurality of first magnetic beads arranged in the first circulated fluid area or the first circulated fluid channel of the circulated fluid body; and a plurality of second magnetic beads arranged in the circulated fluid The second circulating fluid zone or the second circulating fluid channel of the fluid body, so that the first magnetic bead and the second magnetic bead form a first magnetic bead and a second magnetic bead circulating collection body; wherein the circulating collecting body is formed by the first magnetic bead and the second magnetic bead; The fluid body, its first magnetic beads and its second magnetic beads are placed in an environment to collect several microorganisms or biomolecule samples in a circulating flow manner to form a plurality of first microorganisms or biomolecule adsorption magnetic beads and a plurality of second Microbes or biomolecules adsorb the magnetic beads, and a plurality of the first microbes or biomolecules adsorbed magnetic beads and a plurality of the second microbes or biomolecules adsorbed magnetic beads are taken out from the circulated fluid body, and taken out from the plurality of first Microorganisms or health The second microbes or biomolecules adsorbed magnetic beads and a plurality of the second microbes or biomolecules adsorbed magnetic beads are subsequently taken out of the microbes or biomolecule samples. According to the circulatory environment microorganism or biomolecule collection system described in item 1 or 2 of the scope of patent application, the circulated fluid body is selected from a bottle, a box, a tube, a cavity, a sphere, An ellipsoidal sphere, a floating sphere, a buoy body or any combination thereof. According to the recirculating environment microorganism or biomolecule collection system described in item 1 or 2 of the scope of patent application, the circulated fluid body has at least one or several fluid inlets and at least one or several fluid discharge ports. According to the recirculating environment microorganism or biomolecule collection system described in item 1 or 2 of the scope of patent application, the first magnetic bead is selected from a magnetic bead with a chemically modified surface, and the surface of the first magnetic bead can be adsorbed Different microorganisms or biomolecules. A method for collecting microorganisms or biomolecules in a circulating environment, comprising: arranging a plurality of first magnetic beads with a circulating fluid body to form a first magnetic bead circulating collecting body; the circulating fluid body and the second magnetic beads A magnetic bead is placed in an environment to collect several samples of microorganisms or biomolecules in a circulating flow mode to form a plurality of first microorganisms or biomolecules adsorbed magnetic beads; a plurality of the first microorganisms or biomolecules are adsorbed from the magnetic beads Taking out the circulating fluid body; and subsequently taking out several samples of the microorganisms or biomolecules from a plurality of the first microorganisms or biomolecule adsorption magnetic beads. A method for collecting microorganisms or biomolecules in a circulating environment, comprising: arranging a plurality of first magnetic beads and a plurality of second magnetic beads using a circulated fluid body, so that the first magnetic beads and the second magnetic beads form a The first magnetic bead and the second magnetic bead circulating collection body; the recirculating fluid body, the first magnetic bead and the second magnetic bead are placed in one Collect several samples of microorganisms or biomolecules in a circulating flow in the environment to form several first microorganisms or biomolecules adsorbed magnetic beads and several second microorganisms or biomolecules adsorbed magnetic beads; Molecular adsorption magnetic beads and a plurality of the second microorganisms or biomolecule adsorption magnetic beads are taken out from the recirculating fluid body; and a plurality of the first microorganisms or biomolecule adsorption magnetic beads and a plurality of the second microorganisms or biomolecules are adsorbed The magnetic beads subsequently take out several samples of the microorganisms or biomolecules. According to the method for collecting microorganisms or biomolecules in a circulating environment according to item 6 or 7 of the scope of patent application, the body of the circulating fluid is selected from a bottle, a box, a tube, a cavity, a sphere, An ellipsoidal sphere, a floating sphere, a buoy body or any combination thereof. According to the method for collecting microorganisms or biomolecules in a circulating environment according to item 6 or 7 of the scope of the patent application, the circulating fluid body has at least one or several fluid inlets and at least one or several fluid discharge ports. According to the method for collecting microorganisms or biomolecules in a circulating environment according to the scope of patent application 6 or 7, wherein the first magnetic bead is selected from a magnetic bead with a chemically modified surface, and the surface of the first magnetic bead can be adsorbed Different microorganisms or biomolecules.

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