TWI706790B - Hair treatment composition - Google Patents

Abstract

Provided is a hair treatment composition which contains 0.0005 wt% or more of a melanin synthesis stimulating peptide, 0.00069 wt% or more of a hair regrowth peptide, 0.00001 wt% or more of an anti-aging peptide, 0.01525 wt% or more of a stem cell lysate, and 0.00563 wt% or more of an anti-inflammatory plant extract.

Description

Hair treatment composition

The present invention relates to a hair treatment composition.

Hair color is formed by melanin. There are two types of melanin: brown to black eumelanin and yellow to red brown melanin. Hair color is determined by the ratio of eumelanin to pheomelanin in the hair. For example, when there is more eumelanin and less brown melanin, the hair becomes black. When there is less eumelanin and more brown melanin, the hair becomes blond or red. If the melanin contained in the hair decreases or disappears, the hair becomes gray hair or gray hair (for example, refer to Patent Documents 1 to 3).

There are pigment stem cells in the bulge area of the hair follicle. Pigment stem cells produce pigment cells (melanocytes). Pigment cells produce melanin and supply it to hair mother cells (keratinocytes). Hair mother cells supplied with melanin divide into hair and grow out. It is believed that pigment stem cells will decrease or their activity will decrease due to age. It is believed that the decrease in pigment stem cells and the decrease in activity are the cause of gray hair or gray hair. [Prior Technical Literature] [Patent Literature]

[Patent Document 1] Japanese Patent No. 3289975 [Patent Document 2] Japanese Patent No. 4971072 [Patent Document 3] Japanese Patent No. 5850266

[The problem to be solved by the invention]

One of the objectives of the present invention is to provide a hair treatment composition which can efficiently increase the melanin of hair. [Technical means to solve the problem]

According to an aspect of the present invention, there is provided a hair treatment composition comprising more than 0.0005 wt% melanin synthesis promoting peptide, more than 0.00069 wt% hair regeneration peptide, more than 0.00001 wt% anti-aging peptide, and more than 0.01525 wt% stem cells Solubles and anti-inflammatory plant extracts of 0.00563% by weight or more.

In the aforementioned hair treatment composition, the melanin synthesis promoting peptide may be palmitoyltetrapeptide-20 or palmitoyltetrapeptide-20amide.

In the aforementioned hair treatment composition, palmitoyltetrapeptide-20 amide may be contained in Greyverse (registered trademark).

In the above hair treatment composition, the hair regeneration peptide may be acetotetrapeptide-3.

In the above hair treatment composition, the hair regrowth peptide may be contained in Capixyl (registered trademark).

In the above hair treatment composition, the anti-aging peptide may be trifluoroacetyl tripeptide-2.

In the above hair treatment composition, the anti-aging peptide may be contained in Progeline (registered trademark).

In the above hair treatment composition, the stem cell lysate may include apple stem cell lysate.

In the above hair treatment composition, the apple stem cell lysate may be included in an amount of 0.009% by weight or more.

In the above hair treatment composition, the stem cell lysate may include bamboo stem cell lysate.

In the above hair treatment composition, the bamboo stem cell lysate may be included in an amount of 0.00625% by weight or more.

In the above-mentioned hair treatment composition, the anti-inflammatory plant extract may include a Liulan extract.

The above hair treatment composition may contain 0.005% by weight or more of the above willow orchid extract.

In the aforementioned hair treatment composition, the Liulan extract may be contained in Defenscalp (registered trademark).

In the aforementioned hair treatment composition, the anti-inflammatory plant extract may include a flower extract of red clover.

In the above hair treatment composition, the red clover flower extract may be included in an amount of 0.00063% by weight or more.

In the above hair treatment composition, the flower extract of red clover may be contained in Capixyl (registered trademark).

The above hair treatment composition can prevent gray hair or gray hair.

The above hair treatment composition can increase the melanin of the hair.

The aforementioned hair treatment composition may be a shampoo.

The aforementioned hair treatment composition may be a beauty liquid.

According to an aspect of the present invention, there is provided a method for preventing gray hair or gray hair, the method comprising applying the aforementioned hair treatment composition to the scalp.

According to an aspect of the present invention, there is provided a method for increasing the melanin of hair, the method comprising applying the hair treatment composition to the scalp.

According to an aspect of the present invention, there is provided a hair treatment composition comprising 1.0% by weight or more of Greyverse (registered trademark), 2.5% by weight or more of Capixyl (registered trademark), 0.05% by weight or more of Progeline (registered trademark), 0.009 More than weight% of apple stem cell lysate, 0.00625 weight% or more of bamboo stem cell lysate, and 0.1 weight% or more of Defenscalp (registered trademark).

The above hair treatment composition can prevent gray hair or gray hair.

The above hair treatment composition can increase the melanin of the hair.

The aforementioned hair treatment composition may be a shampoo.

The aforementioned hair treatment composition may be a beauty liquid. [Effects of Invention]

According to the present invention, a hair treatment composition can be provided, which can efficiently increase the melanin of hair.

Hereinafter, embodiments of the present invention will be described in detail. In addition, the embodiment shown below is an example of a device or method for embodying the technical idea of the invention, and the technical idea of the invention does not limit the combination of constituent materials and the like to the following. Various changes can be added to the technical idea of the invention within the scope of the patent application.

The hair treatment composition of the embodiment includes 0.0005 wt% or more of melanin synthesis promoting peptide, 0.00069 wt% or more of hair regeneration peptide, 0.00001 wt% or more of anti-aging peptide, 0.01525 wt% or more of dry cell lysate, and 0.00563 wt% or more. Anti-inflammatory plant extracts.

The concentration of the melanin synthesis promoting peptide in the hair treatment composition of the embodiment may be 0.0005% by weight or more, 0.00065% by weight or more, or 0.0008% by weight or more. In addition, the concentration of the melanin synthesis promoting peptide may be 0.0025% by weight or less, 0.002% by weight or less, or 0.0015% by weight or less. Melanin synthesis promoting peptide promotes the synthesis of melanin. The melanin synthesis promoting peptide binds to melanocortin 1 receptor (MC1-R), for example. In addition, the melanin synthesis promoting peptide can reduce the oxidative pressure of the melanin that causes gray hair or gray hair. For example, melanin synthesis promoting peptides can activate deacetylase (SIRT), which is a long-lived protein, and tyrosine kinase-related protein (TRP)-2, which is an antioxidant enzyme.

Examples of melanin synthesis promoting peptides include palmitoyl tetrapeptide-20, palmitoyl tetrapeptide-20 amide, and acethexapeptide-1. Palmitoyl tetrapeptide-20 is a reaction product of palmitic acid and a synthetic tetrapeptide composed of arginine, histidine, phenylalanine and tryptophan. Palmitoyl Tetrapeptide-20 is a biomimetic peptide of α-melanocyte-stimulating hormone (MSH). Palmitoyl tetrapeptide-20 amide can be included in Greyverse (registered trademark, AH&NS).

The concentration of Greyverse (registered trademark, AH&NS) in the hair treatment composition of the embodiment may be 1.0% by weight or more, 1.25% by weight or more, or 1.5% by weight or more. In addition, the concentration of Greyverse (registered trademark, AH&NS) may be 6.0% by weight or less, 5.5% by weight or less, or 5.0% by weight or less.

The concentration of the hair regenerative peptide in the hair treatment composition of the embodiment may be 0.00069% by weight or more, 0.0009% by weight or more, or 0.0011% by weight or more. In addition, the concentration of the hair regenerative peptide may be 0.00345% by weight or less, 0.00276% by weight or less, or 0.00207% by weight or less. Hair regeneration peptides can be peptides that protect hair follicles and hair roots. In addition, the hair regenerative peptide can have an anti-inflammatory effect that inhibits inflammation that causes gray hair or gray hair. The hair regeneration peptide can be a peptide that inhibits 5α reductase and inhibits male hormones.

As examples of hair regeneration peptides, acetotetrapeptide-3 and tripeptide-1 copper can be cited. Acetyltetrapeptide-3 is obtained by acetylating synthetic tetrapeptides including lysine, glycine, histidine and lysine. Acetatetetrapeptide-3 can be contained in Capixyl (registered trademark, AH&NS).

The concentration of Capixyl (registered trademark, AH&NS) in the hair treatment composition of the embodiment may be 2.5% by weight or more, 3.125% by weight or more, or 3.75% by weight or more. In addition, the concentration of Capixyl (registered trademark, AH&NS) may be 15.0% by weight or less, 13.75% by weight or less, or 12.5% by weight or less.

The concentration of the anti-aging peptide in the hair treatment composition of the embodiment can be 0.00001% by weight or more, 0.00001% by weight or more, or 0.00002% by weight or more. In addition, the concentration of the anti-aging peptide may be 0.00005% by weight or less, 0.00004% by weight or less, or 0.00003% by weight or less. Anti-aging peptides, for example, increase the tolerance of hair follicle cells and dermal papilla cells to stress hormones. Anti-aging peptides may have the function of inhibiting inflammation that causes gray hair or gray hair. Due to the presence of hair regenerative peptides and anti-aging peptides, it has a tendency to inhibit neutrophil elastase and protect hair follicles from stress hormones.

As an example of the anti-aging peptide, trifluoroacetyl tripeptide-2 can be cited. Trifluoroacetate tripeptide-2 can be obtained from tripeptide-2 containing valine, tyrosine and valine and trifluoroacetic acid. Trifluoroacetyl tripeptide-2 can be included in Progeline (registered trademark, AH&NS).

The concentration of Progeline (registered trademark, AH&NS) in the hair treatment composition of the embodiment may be 0.05% by weight or more, 0.0625% by weight or more, or 0.075% by weight or more. In addition, the concentration of Progeline (registered trademark, AH&NS) may be 0.3% by weight or less, 0.275% by weight or less, or 0.25% by weight or less.

The concentration of the stem cell lysate in the hair treatment composition of the embodiment can be 0.01525% by weight or more, 0.0176% by weight or more, or 0.02432% by weight or more. In addition, the concentration of the dry cell lysate may be 0.076% by weight or less, 0.061% by weight or less, or 0.0456% by weight or less. The stem cell lysate may be an extract of stem cells. The stem cell lysate contains at least the lysate of the cytoplasmic component of stem cells. In addition to the lysate of the cytoplasmic components of the stem cells, the stem cell lysate may also include the lysate of the cell wall components of the stem cells.

The stem cell lysate may include apple stem cell lysate. The apple stem cell lysate can be an extract of apple stem cells. Apple stem cells have the effect of maintaining melanin in the hair roots. The apple stem cell lysate contains at least the lysate of the cytoplasmic components of apple stem cells. In addition to the lysate of the cytoplasmic components of apple stem cells, the lysate of apple stem cells may also include the lysate of the cell wall components of apple stem cells. Apple stem cell lysate protects the scalp, protects hair follicles, and activates bulge stem cells. Due to the presence of hair regeneration peptides and apple stem cell lysates, there is a tendency to increase the protective effect of hair roots and the promotion of collagen 17 production. The method for producing the apple stem cell lysate is arbitrary. For example, the apple stem cell lysate can be obtained by applying high pressure to the apple stem cells. The lysate of apple stem cells can be contained in a solvent containing lecithin, glycerin, sanxian gum and phenoxyethanol.

The concentration of the lysate of apple stem cells in the hair treatment composition of the embodiment may be 0.009% by weight or more, 0.0117% by weight or more, or 0.0144% by weight or more. In addition, the concentration of the lysate of apple stem cells may be 0.045% by weight or less, 0.036% by weight or less, or 0.027% by weight or less. As an apple stem cell lysate, for example, PhytoCellTec Malus Domestica (registered trademark, AH&NS) can be used.

The stem cell lysate may include bamboo stem cell lysate. The bamboo stem cell lysate can be an extract of bamboo stem cells. As bamboo stem cells, Mengzong bamboo growth point stem cells can be used. The bamboo stem cell lysate contains at least the lysate of the cytoplasmic components of bamboo stem cells. In addition to the lysate of the cytoplasmic components of the bamboo stem cells, the bamboo stem cell lysate may also include the lysate of the cell wall components of the bamboo stem cells. The method of manufacturing bamboo stem cell lysate is arbitrary, for example, the cytoplasmic water solubles of bamboo stem cells can be extracted.

The concentration of the bamboo stem cell lysate in the hair treatment composition of the embodiment may be 0.00625% by weight or more, 0.00813% by weight or more, or 0.01% by weight or more. In addition, the concentration of the bamboo stem cell lysate may be 0.03125 wt% or less, 0.025 wt% or less, or 0.01875 wt% or less. The bamboo stem cell lysate has anti-glycation effect, promotes the production of collagen, and derives keratin. Furthermore, if the scalp is glycated, the scalp tends to become hard. Due to the presence of bamboo stem cell lysate, hair regeneration peptide and apple stem cell lysate, there is a tendency to increase anti-glycation and keratin-derived effects. The bamboo stem cell lysate can be obtained from AH&NS, for example.

The concentration of the anti-inflammatory plant extract in the hair treatment composition of the embodiment can be 0.00563% by weight or more, 0.00732% by weight or more, or 0.00901% by weight or more. In addition, the concentration of the anti-inflammatory plant extract may be 0.02815% by weight or less, 0.02252% by weight or less, or 0.01689% by weight or less. Anti-inflammatory plant extracts can protect hair roots. Due to the presence of hair regenerative peptides and anti-inflammatory plant extracts, there is a tendency for anti-inflammatory effects and hair root protection to increase.

As an example of anti-inflammatory plant extracts, Liulan extract can be cited. The Liulan extract may be the flower extract of Liulan, the leaf extract of Liulan, and the stem extract of Liulan, and mixtures of these. Willow orchid extract maintains the proper amount of Malassezia, which is an inherent scalp bacteria, and protects the hair roots. Willow orchid extract contains Oenothein B (Oenothein B) shown in the following chemical formula as an active ingredient. [化1]

The concentration of the Liulan extract in the hair treatment composition of the embodiment may be 0.005% by weight or more, 0.0065% by weight or more, or 0.008% by weight or more. In addition, the concentration of the Liulan extract in the hair treatment composition of the embodiment may be 0.025% by weight or less, 0.02% by weight or less, or 0.015% by weight or less. Willow orchid extract can be contained in Defenscalp (registered trademark, AH&NS).

The concentration of Defenscalp (registered trademark, AH&NS) in the hair treatment composition of the embodiment may be 0.1% by weight or more, 0.125% by weight or more, or 0.15% by weight or more. In addition, the concentration of Defenscalp (registered trademark, AH&NS) may be 0.6% by weight or less, 0.55% by weight or less, or 0.5% by weight or less.

In addition, as examples of anti-inflammatory plant extracts, red clover flower extracts and avocado oil fatty acid butyl ester can be cited. Preferably, in the hair treatment composition of the embodiment, a red clover flower extract or avocado oil fatty acid butyl ester is formulated together with the hair regeneration peptide. Red clover has the effect of inhibiting interleukin 8, which can damage dermal papilla cells. In addition, red clover contains biochanin A represented by the following chemical formula. [化2]

The concentration of the red clover flower extract in the hair treatment composition of the embodiment may be 0.00063% by weight or more, 0.00082% by weight or more, or 0.001% by weight or more. In addition, the concentration of the flower extract of red clover may be 0.00315% by weight or less, 0.00232% by weight or less, and 0.00189% by weight or less. The flower extract of red clover can be contained in Capixyl (registered trademark, AH&NS) or Miniporyl (registered trademark, AH&NS).

The hair treatment composition of the embodiment can be shampoo, beauty lotion, hair conditioner, gel, hair care agent, hair cream, tincture, spray, mousse, cleansing composition, hair conditioner, hair care lotion , Hair cream and foam, etc. The hair treatment composition of the embodiment is applied to the scalp more than once a day, for example.

The hair treatment composition of the embodiment may include, for example, water, C1-C4-alkanol and other water-soluble organic solvents; oils; fatty substances; waxes; esters of C6-C30 monocarboxylic acids and mono-, di-, or tri-alcohols ; Saturated acyclic and cyclic hydrocarbons; fatty acids; aliphatic alcohols; silicone oils; surface active ingredients; defoamers; propellants; defoamers; emulsifiers; foaming agents; and co-solvents. The interface active ingredients can be anionic, cationic, amphoteric and neutral. The emulsifier can be anionic, cationic, amphoteric and neutral.

The hair treatment composition of the embodiment may include, for example, a water-soluble or water-dispersible hair polymer. Examples of hair polymers include modeling polymers and conditioning polymers. More specifically, examples of hair polymers include: copolymers of vinyl acetate and crotonic acid, copolymers of methyl vinyl ether and maleic anhydride, copolymers of acrylic acid or methacrylic acid and other monomers Polyurethane, polyurethane, N-vinylpyrrolidone and silicone polymer.

The hair treatment composition of the embodiment may include, for example, water-insoluble polysiloxane such as polydimethylsiloxane. The hair treatment composition of the embodiment may contain wax such as fatty amide, for example.

The hair treatment composition of the embodiment may contain an antioxidant, for example. The above-mentioned Liulan extract and red clover flower extract can also function as antioxidants. Examples of other antioxidants include hemoglobin, vitamin C, and vitamin E.

The hair treatment composition of the embodiment may include, for example, a preservative, an antioxidant, an aromatic oil, a lipid re-fatification agent, an active ingredient, and a care ingredient. For example, the hair treatment composition of the embodiment may include panthenol, collagen, vitamins, protein hydrolysates, α and β hydroxycarbonic acid, stabilizers, pH adjusters, opacifiers, colorants, dyes, gel forming agents, Salt, humectant, complexing agent, viscosity regulator and sunscreen.

The hair treatment composition of the embodiment may include, for example, polyalkylsiloxane, polyarylsiloxane, polyaralkylsiloxane, polysiloxane resin, polyethersiloxane, and dimethylpolysiloxane copolymer. Alcohol (CTFA) and amine functionalized polysiloxane compound.

When the hair treatment composition of the embodiment includes a propellant, the hair treatment composition of the embodiment may include, for example, propane/butane, pentane, dimethyl ether, 1,1-difluoroethane (HFC-152a) , Carbon dioxide, nitrogen and compressed air mixture.

When the hair treatment composition of the embodiment includes a nonionic emulsifier, the hair treatment composition of the embodiment may include, for example, laureth-4 such as laureth-4, ceteth-1, etc. Alcohol, polyethylene glycol cetyl ether, ceteareth-25 and other cetearyl alcohol polyethers, polyglycol fatty acid glycerides, hydroxylated lecithin, lactic acid esters and alkyl groups of fatty acids Polyglycoside.

When the hair treatment composition of the embodiment includes a nonionic surfactant, the hair treatment composition of the embodiment may include, for example, aliphatic alcohol or a linear or branched alkyl chain having 6 to 20 carbon atoms. The reaction product of alkylphenol and ethylene oxide and/or propylene oxide.

The hair treatment composition of the embodiment may include, for example, alkyl amine oxide, mono- or dialkyl alkanol amide, fatty acid ester of polyethylene glycol, alkyl polyglycoside, and sorbitan ester.

When the hair treatment composition of the embodiment includes a cationic emulsifier and/or a cationic surfactant, the hair treatment composition of the embodiment may include, for example, a quaternized ammonium compound. The hair treatment composition of the embodiment may include, for example, cetyl trimethyl ammonium chloride or bromide, stearyl benzyl dimethyl ammonium chloride, distearyl dimethyl ammonium chloride, and stearyl amine Propyl dimethyl amine, hydroxyethyl cetyl dimethyl ammonium phosphate, poly(oxy-1,2-ethanediyl), and (octadecyl nitrogen) tri-2,1-( Ethane diyl) tris-(hydroxy)-phosphate. The hair treatment composition of the embodiment may include, for example, a cationic guar derivative such as guar hydroxypropyltrimethylammonium chloride.

When the hair treatment composition of the embodiment includes an anionic emulsifier and/or an anionic surfactant, the hair treatment composition of the embodiment may include, for example, alkyl sulfate, alkyl ether sulfate, and alkyl sulfonic acid. Salt, alkyl aryl sulfonate, alkyl succinate, alkyl sulfosuccinate, N-alkyl sarcosine, acylotaurate, acylate isethionate, alkane Alkyl phosphate, alkyl ether phosphate, alkyl ether carboxylate, and α-olefin sulfonate.

When the hair treatment composition of the embodiment includes an anionic surfactant, the hair treatment composition of the embodiment may include, for example, sodium lauryl sulfate, ammonium lauryl sulfate, sodium laureth sulfate, ammonium laureth sulfate, and laurel Sodium sarcosine, sodium oleyl succinate, ammonium lauryl sulfosuccinate, sodium dodecyl benzene sulfonate and triethanolamide decyl benzene sulfonate.

When the hair treatment composition of the embodiment includes an amphoteric surfactant, the hair treatment composition of the embodiment may include, for example, alkyl betaine, alkyl amidopropyl betaine, alkyl sulfobetaine, and alkyl sulfobetaine. Glycine, alkylcarboxyglycine, alkyl amphoteric acetate or propionate, and alkyl amphoteric diacetate or dipropionate.

The hair treatment composition of the embodiment can increase the melanin of the hair and prevent gray hair or gray hair. The hair treatment composition of the embodiment can restore the hair color to the color before turning white or gray hair.

By applying the hair treatment composition of the embodiment to the scalp, the melanin synthesis promoting peptide in the hair follicle can promote the synthesis of melanin, but is not bound by theory. Even if the synthesis of melanin is promoted, if thick hair is not grown, gray or gray hair cannot be effectively restored to hair containing melanin. In this regard, hair regenerative peptides and stem cell lysates promote thickening and growth of hair containing melanin. Furthermore, anti-aging peptides increase the tolerance of hair follicle cells and dermal papilla cells to stress hormones, and anti-inflammatory plant extracts inhibit inflammation in hair follicles, thereby effectively returning gray or gray hair to hair containing melanin.

As described above, the present invention has been described in the embodiments, but it should not be understood that the description and drawings as part of the disclosure limit the invention. For those in the industry, it should be possible to understand various alternative embodiments, examples, and operating technologies from the disclosure content. It should be understood that the present invention includes various embodiments that are not described herein.

(Example)

Prepare the fragrance (beauty lotion) of the example, which contains 0.001% by weight of palmitoyl tetrapeptide-20, 0.00138% by weight of acetyltetrapeptide-3, 0.00125% by weight of red clover flower extract, and 0.00002% by weight of trifluoride Acetyl tripeptide-2, 0.018% by weight of apple fruit cultured stem cell extract, 0.0125% by weight of Mengzong bamboo growing point stem cell lysate, and 0.01% by weight of willow flower/leaf/stem extract.

Palmitoyl Tetrapeptide-20 is included in Greyverse (registered trademark). The content of Greyverse (registered trademark) in the flavors of the examples is 2.0% by weight. Acetotetrapeptide-3 and red clover flower extract are contained in Capixyl (registered trademark). The content of Capixyl (registered trademark) in the flavors of the examples is 5.0% by weight. Trifluoroacetate tripeptide-2 is included in Progeline (registered trademark). The content of Progeline (registered trademark) in the flavors of the examples is 0.1% by weight. Apple fruit culture stem cell extract and Mengzong bamboo growing point stem cell lysate were purchased from AH&NS. Willow flower/leaf/stem extract is contained in Defenscalp (registered trademark). The content of Defenscalp (registered trademark) in the flavors of the examples is 0.2% by weight.

Twenty adults (average age 43) between 20 and 69 years old are selected as subjects. Before the subject starts to use the fragrance of the embodiment, the doctor and the hair diagnostician observe the white hair on the top and side of the subject's head. In addition, doctors and hair diagnosticians use microscopes to observe the hair color at the roots of the subject's head (the hairline at the top of the helix).

Thereafter, the subjects used shampoo containing antioxidants once a day for 3 months to wash their hair, and applied the fragrance of the example to the entire scalp. During this period, the subjects were prohibited from using other shampoos, hair cosmetics, white hair dyes and hair dyes. After starting to use the fragrance of the embodiment, the doctor and the hair diagnostician observe the subject's hair in the first month, the second month, and the third month. Furthermore, two months after starting to use the fragrance of the example, one subject did not conduct the test due to personal reasons.

Fig. 1 and Table 1 show the transition of the degree of improvement in the amount of gray hair of the subject observed by the doctor and the hair diagnostician. Furthermore, when the amount of gray hair decreases, it is evaluated as improvement. Regarding the amount of gray hair on the top of the head, compared with before use, one month after the start of use, "unchanged" was 8 of 20 (40%), and "slightly improved" was 5 (25%) of 20. , "Improved" is 7 out of 20 (35%). Regarding the amount of gray hair on the top of the head, compared with before use, after 2 months of use, "unchanged" was 5 out of 20 (25%), and "slightly improved" was 10 out of 20 (50%). , "Improved" is 5 out of 20 (25%). Regarding the amount of gray hair on the top of the head, compared with before use, after 3 months of use, "slightly improved" was 8 out of 19 (42%), and "improved" was 11 out of 19 (58%). [Table 1] A table showing the transition of the rate of improvement in the amount of gray hair evaluated by the physicians and hair diagnosticians in the examples Gray hair Top of head Side head ID 0M 1M 2M 3M 0M 1M 2M 3M E-01 - improve constant Slightly improved - improve Slightly improved Slightly improved E-02 - improve constant Slightly improved - improve Slightly improved Slightly improved E-03 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved E-04 - constant Slightly improved improve - constant Slightly improved Slightly improved E-05 - improve improve improve - improve Slightly improved improve E-06 - improve Slightly improved improve - Slightly improved improve improve E-07 - improve Slightly improved improve - improve Slightly improved Slightly improved E-08 - constant Slightly improved Slightly improved - constant constant improve E-09 - constant Slightly improved improve - constant Slightly improved Slightly improved E-10 - improve improve - - improve Slightly improved - E-11 - Slightly improved constant Slightly improved - Slightly improved Slightly improved Slightly improved E-12 - constant constant Slightly improved - constant Slightly improved Slightly improved E-13 - constant constant Slightly improved - constant Slightly improved Slightly improved E-14 - Slightly improved Slightly improved improve - improve Slightly improved Slightly improved E-15 - Slightly improved Slightly improved improve - Slightly improved Slightly improved improve E-16 - constant improve improve - constant Slightly improved improve E-17 - Slightly improved Slightly improved improve - Slightly improved improve improve E-18 - constant improve improve - constant improve improve E-19 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved improve E-20 - improve improve improve - improve Slightly improved improve n % n % n % n % n % n % Significant improvement - 0 0 0 0 0 0 - 0 0 0 0 0 0 improve - 7 35 5 25 11 58 - 7 35 3 15 9 47 Slightly improved - 5 25 10 50 8 42 - 5 25 16 80 10 53 constant - 8 40 5 25 0 0 - 8 40 1 5 0 0 deterioration - 0 0 0 0 0 0 - 0 0 0 0 0 0 M = months later

Regarding the amount of gray hair on the side of the head, compared with before use, one month after the start of use, "unchanged" was 8 out of 20 (40%), and "slightly improved" was 5 out of 20 (25%) ), "Improved" is 7 out of 20 (35%). Regarding the amount of gray hair on the lateral head, compared with before use, 2 months after the start of use, "unchanged" was 1 out of 20 (5%), and "slightly improved" was 16 out of 20 (80%) ), "improvement" is 3 out of 20 (15%). Regarding the amount of gray hair on the side of the head, compared with before use, after 3 months of use, "slightly improved" was 10 out of 19 (53%), and "improved" was 9 out of 19 (47%). .

Fig. 2 and Table 2 show the transition of the degree of improvement of the subject’s gray hair color observed by the physician and the hair diagnostician. In addition, when the white hair is colored, it is evaluated as an improvement. Regarding the white hair color on the top of the head, compared with before use, after 1 month of use, the "unchanged" was 8 out of 20 (40%), and "slightly improved" was 7 out of 20 (35%). , "Improved" is 5 out of 20 (25%). Regarding the white hair color on the top of the head, compared with before use, after 2 months of use, "unchanged" was 2 of 20 (10%), and "slightly improved" was 15 of 20 (75%). , "Improved" is 3 out of 20 (15%). Regarding the white hair color on the top of the head, compared with before use, after 3 months of use, "slightly improved" was 10 out of 19 (53%), and "improved" was 9 out of 19 (47%). [Table 2] A table showing the transition of the gray hair color improvement rate evaluated by the physicians and hair diagnosticians in the examples Gray hair color Top of head Side head ID 0M 1M 2M 3M 0M 1M 2M 3M E-01 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved E-02 - improve Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved E-03 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved E-04 - constant Slightly improved improve - constant Slightly improved improve E-05 - improve improve improve - improve improve improve E-06 - improve improve improve - Slightly improved Slightly improved Slightly improved E-07 - improve Slightly improved improve - improve Slightly improved improve E-08 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved E-09 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved E-10 - improve Slightly improved - - improve Slightly improved - E-11 - Slightly improved constant Slightly improved - Slightly improved constant Slightly improved E-12 - constant Slightly improved improve - constant Slightly improved Slightly improved E-13 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved E-14 - Slightly improved Slightly improved improve - Slightly improved Slightly improved Slightly improved E-15 - Slightly improved constant Slightly improved - Slightly improved Slightly improved Slightly improved E-16 - constant Slightly improved improve - constant Slightly improved Slightly improved E-17 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved E-18 - constant improve improve - constant improve improve E-19 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved improve E-20 - Slightly improved Slightly improved improve - Slightly improved Slightly improved improve n % n % n % n % n % n % Significant improvement - 0 0 0 0 0 0 - 0 0 0 0 0 0 improve - 5 25 3 15 9 47 - 3 15 2 10 6 32 Slightly improved - 7 35 15 75 10 53 - 9 45 17 85 13 68 constant - 8 40 2 10 0 0 - 8 40 1 5 0 0 deterioration - 0 0 0 0 0 0 - 0 0 0 0 0 0 M = months later

Regarding the white hair color on the side of the head, compared with before use, after 1 month of use, "unchanged" was 8 of 20 (40%), and "slightly improved" was 9 of 20 (45%) ), "improvement" is 3 out of 20 (15%). Regarding the white hair color on the side of the head, compared with before use, after 2 months of use, "unchanged" was 1 in 20 (5%), and "slightly improved" was 17 in 20 (85%) ), "improvement" is 2 out of 20 (10%). Regarding the white hair color on the side of the head, compared with before use, after 3 months of use, "slightly improved" was 13 out of 19 (68%), and "improved" was 6 out of 19 (32%). .

Fig. 3 and Table 3 show the progress of the improvement rate of the white hair roots of the subjects observed by the doctor and the hair diagnostician. Furthermore, when the roots of white hair turned gray, it was evaluated as improvement. Regarding the condition of the white hair roots on the top of the head, compared with before use, one month after the start of use, "unchanged" was 8 of 20 (40%), and "slightly improved" was 5 of 20 (25 %), "improvement" is 7 out of 20 (35%). Regarding the condition of the roots of the white hair on the top of the head, compared with the condition before use, after 2 months of use, the "unchanged" was 3 out of 20 (15%), and "slightly improved" was 14 out of 20 (70 %), "improvement" is 3 out of 20 (15%). Regarding the condition of the white hair roots on the top of the head, compared with before use, after 3 months of starting use, "slightly improved" was 10 out of 19 (53%), and "improved" was 9 out of 19 (47%) ). [Table 3] A table showing the transition of the rate of improvement of the gray hair roots evaluated by the physicians and hair diagnosticians of the examples The state of white hair roots Top of head Side head ID 0M 1M 2M 3M 0M 1M 2M 3M E-01 - improve Slightly improved Slightly improved - improve Slightly improved Slightly improved E-02 - improve Slightly improved improve - improve improve improve E-03 - constant Slightly improved Slightly improved - constant improve improve E-04 - constant improve improve - constant improve improve E-05 - improve Slightly improved improve - improve Slightly improved improve E-06 - improve Slightly improved improve - Slightly improved Slightly improved Slightly improved E-07 - improve improve Slightly improved - improve Slightly improved Slightly improved E-08 - constant Slightly improved Slightly improved - constant Slightly improved improve E-09 - constant Slightly improved improve - constant Slightly improved Slightly improved E-10 - improve Slightly improved - - improve improve - E-11 - Slightly improved constant Slightly improved - Slightly improved Slightly improved Slightly improved E-12 - constant Slightly improved improve - constant improve improve E-13 - constant constant Slightly improved - constant improve improve E-14 - Slightly improved Slightly improved improve - constant Slightly improved improve E-15 - Slightly improved constant Slightly improved - Slightly improved constant Slightly improved E-16 - constant Slightly improved improve - constant Slightly improved Slightly improved E-17 - Slightly improved Slightly improved Slightly improved - Slightly improved improve improve E-18 - constant Slightly improved Slightly improved - constant Slightly improved improve E-19 - Slightly improved Slightly improved improve - Slightly improved improve improve E-20 - improve improve Slightly improved - improve Slightly improved Slightly improved n % n % n % n % n % n % Significant improvement - 0 0 0 0 0 0 - 0 0 0 0 0 0 improve - 7 35 3 15 9 47 - 6 30 8 40 11 58 Slightly improved - 5 25 14 70 10 53 - 5 25 11 55 8 42 constant - 8 40 3 15 0 0 - 9 45 1 5 0 0 deterioration - 0 0 0 0 0 0 - 0 0 0 0 0 0 M = months later

Regarding the condition of the white hair roots on the side head, compared with before use, after 1 month of use, "unchanged" was 9 out of 20 (45%), and "slightly improved" was 5 out of 20 ( 25%), "improvement" is 6 out of 20 (30%). Regarding the condition of the white hair roots on the side head, compared with before use, after 2 months of use, "unchanged" was 1 in 20 (5%), and "slightly improved" was 11 in 20 ( 55%), "Improved" is 8 out of 20 (40%). Regarding the condition of the white hair roots on the side head, compared with before use, after 3 months of use, "slightly improved" was 8 out of 19 (42%), and "improved" was 11 out of 19 (58 %).

不擔心（10）]白髮） ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS平均值 0M 1.3 8.5 2.3 9.7 1.5 5.1 5.0 5.2 1.7 1.9 0.0 0.0 8.4 4.5 8.9 9.2 5.5 9.9 0.4 2.4 4.6 1M 1.4 8.8 2.1 9.6 1.4 4.6 5.3 4.4 2.7 1.1 0.0 0.0 8.4 8.6 9.0 9.5 5.2 10.0 1.9 3.2 4.9 2M 2.0 8.9 3.7 9.6 2.8 6.9 6.6 6.7 4.9 2.7 0.9 0.4 8.8 8.8 9.4 9.6 6.4 10.0 3.0 4.1 5.8 3M 3.2 9.3 6.3 9.4 6.1 8.7 8.0 8.2 7.5 - 3.2 2.4 9.5 9.4 9.5 9.7 8.7 10.0 6.1 5.5 7.4 Regarding concerns about gray hair, a questionnaire survey using the Visual Analog Scale (VAS) was conducted on the subjects. In the VAS, the evaluation value when you are worried about gray hair is set to 0, and the evaluation value when you are not worried about gray hair is set to 10. As a result, as shown in Figure 4 and Table 4, it is shown that after starting to use the fragrance of the example, there is no longer worry about gray hair. Also, regarding the rate of change (%) of VAS, when it is greater than 105%, it means improvement, and when it is less than 95%, it means deterioration. As shown in Fig. 5, it shows that after starting to use the fragrance of the embodiment, the condition of gray hair is worried about improvement. [Table 4] A table showing the transition of the subject's concerns about gray hair obtained by the questionnaire survey of the embodiment ([Worry (0)

Don't worry (10)] Gray hair) ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS average 0M 1.3 8.5 2.3 9.7 1.5 5.1 5.0 5.2 1.7 1.9 0.0 0.0 8.4 4.5 8.9 9.2 5.5 9.9 0.4 2.4 4.6 1M 1.4 8.8 2.1 9.6 1.4 4.6 5.3 4.4 2.7 1.1 0.0 0.0 8.4 8.6 9.0 9.5 5.2 10.0 1.9 3.2 4.9 2M 2.0 8.9 3.7 9.6 2.8 6.9 6.6 6.7 4.9 2.7 0.9 0.4 8.8 8.8 9.4 9.6 6.4 10.0 3.0 4.1 5.8 3M 3.2 9.3 6.3 9.4 6.1 8.7 8.0 8.2 7.5 - 3.2 2.4 9.5 9.4 9.5 9.7 8.7 10.0 6.1 5.5 7.4

減少（10）]） ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS平均值 0M 1.2 5.0 4.3 4.8 4.9 3.9 1.4 3.9 2.5 5.2 4.8 0.0 2.9 5.7 5.0 5.0 5.3 5.1 0.4 3.7 3.8 1M 1.4 4.9 4.8 4.5 4.8 4.7 1.1 3.5 3.4 3.9 2.8 4.3 3.3 8.2 5.7 5.6 5.2 4.9 1.7 4.0 4.1 2M 1.8 6.0 5.4 5.7 5.2 7.2 2.7 5.0 4.9 3.5 2.6 4.3 4.2 8.8 7.3 7.1 5.9 6.8 3.8 4.9 5.2 3M 3.3 8.0 7.3 8.1 8.5 8.7 4.2 7.0 7.6 - 4.0 6.3 7.5 9.3 8.8 8.6 8.5 8.0 5.9 5.7 7.1 Regarding the amount of gray hair, a questionnaire using VAS was conducted on the subjects. In VAS, the evaluation value when the amount of gray hair increases is set to 0, and the evaluation value when the amount of gray hair decreases is set to 10. As a result, as shown in Fig. 6 and Table 5, it was shown that the amount of gray hair decreased after starting to use the fragrance of the example. Moreover, as shown in Fig. 7, it is shown that the amount of gray hair has been improved after starting to use the fragrance of the example. [Table 5] A table showing the transition of the amount of gray hair of the subject obtained by the questionnaire survey of the embodiment (the amount of gray hair [increase (0)

Reduce(10)]) ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS average 0M 1.2 5.0 4.3 4.8 4.9 3.9 1.4 3.9 2.5 5.2 4.8 0.0 2.9 5.7 5.0 5.0 5.3 5.1 0.4 3.7 3.8 1M 1.4 4.9 4.8 4.5 4.8 4.7 1.1 3.5 3.4 3.9 2.8 4.3 3.3 8.2 5.7 5.6 5.2 4.9 1.7 4.0 4.1 2M 1.8 6.0 5.4 5.7 5.2 7.2 2.7 5.0 4.9 3.5 2.6 4.3 4.2 8.8 7.3 7.1 5.9 6.8 3.8 4.9 5.2 3M 3.3 8.0 7.3 8.1 8.5 8.7 4.2 7.0 7.6 - 4.0 6.3 7.5 9.3 8.8 8.6 8.5 8.0 5.9 5.7 7.1

變深（10）]） ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS平均值 0M 3.5 5.1 5.0 4.9 5.0 4.8 1.3 4.9 8.7 4.5 4.8 4.7 4.6 1.1 4.9 4.8 5.3 5.2 7.5 2.5 4.7 1M 1.3 4.9 5.2 4.6 4.9 5.0 1.6 5.9 7.2 4.8 6.4 4.5 4.8 0.9 4.9 5.5 5.3 5.1 7.3 3.5 4.7 2M 2.2 5.8 6.5 6.0 5.4 7.2 2.8 6.6 7.7 5.4 6.7 4.6 5.1 3.1 6.7 7.0 6.6 6.5 7.6 5.8 5.8 3M 3.9 7.5 8.0 8.3 8.4 8.6 5.2 8.1 9.1 - 8.2 6.8 8.0 6.5 8.4 8.5 8.4 7.8 8.3 6.7 7.6 Regarding the color of gray hair, a questionnaire survey using VAS was conducted on the subjects. In VAS, the evaluation value when the white hair color becomes lighter is set to 0, and the evaluation value when the white hair color becomes darker is set to 10. The results, as shown in Fig. 8 and Table 6, indicate that the white hair became darker after starting to use the fragrance of the example. In addition, as shown in Fig. 9, it is shown that the white hair color has improved after starting to use the fragrance of the example. [Table 6] A table showing the transition of the white hair color of the subject obtained by the questionnaire survey of the embodiment (white hair color [to light (0)

Deeper (10)]) ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS average 0M 3.5 5.1 5.0 4.9 5.0 4.8 1.3 4.9 8.7 4.5 4.8 4.7 4.6 1.1 4.9 4.8 5.3 5.2 7.5 2.5 4.7 1M 1.3 4.9 5.2 4.6 4.9 5.0 1.6 5.9 7.2 4.8 6.4 4.5 4.8 0.9 4.9 5.5 5.3 5.1 7.3 3.5 4.7 2M 2.2 5.8 6.5 6.0 5.4 7.2 2.8 6.6 7.7 5.4 6.7 4.6 5.1 3.1 6.7 7.0 6.6 6.5 7.6 5.8 5.8 3M 3.9 7.5 8.0 8.3 8.4 8.6 5.2 8.1 9.1 - 8.2 6.8 8.0 6.5 8.4 8.5 8.4 7.8 8.3 6.7 7.6

減少（10）]） ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS平均值 0M 2.2 5.1 3.3 4.9 5.2 4.5 2.4 7.8 3.2 4.5 4.8 0.0 4.1 7.9 5.0 4.9 5.3 5.2 0.8 2.9 4.2 1M 1.4 5.1 3.3 4.6 5.0 5.0 2.5 5.1 3.5 4.9 5.0 4.6 5.2 8.6 5.3 5.5 5.4 5.2 1.8 3.7 4.5 2M 2.1 5.4 4.1 6.2 6.9 7.2 3.7 5.6 4.8 5.7 6.1 4.7 6.5 8.6 6.5 6.7 6.5 6.3 4.0 5.5 5.7 3M 4.2 7.0 6.5 7.5 8.1 8.3 5.9 7.3 8.8 - 7.7 6.8 7.9 9.4 8.3 8.1 8.4 7.5 6.5 6.6 7.4 Regarding the scope of gray hair, a questionnaire survey on the use of VAS was conducted on the subjects. In VAS, the evaluation value when the gray hair range increases is set to 0, and the evaluation value when the gray hair range decreases is set to 10. As a result, as shown in Fig. 10 and Table 7, it is shown that the gray hair range is reduced after starting to use the fragrance of the example. Moreover, as shown in Fig. 11, it is shown that after starting to use the fragrance of the example, the gray hair range has been improved. [Table 7] A table showing the transition of the gray hair range of the subject obtained by the questionnaire survey of the embodiment (white hair range [increase (0)

Reduce(10)]) ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS average 0M 2.2 5.1 3.3 4.9 5.2 4.5 2.4 7.8 3.2 4.5 4.8 0.0 4.1 7.9 5.0 4.9 5.3 5.2 0.8 2.9 4.2 1M 1.4 5.1 3.3 4.6 5.0 5.0 2.5 5.1 3.5 4.9 5.0 4.6 5.2 8.6 5.3 5.5 5.4 5.2 1.8 3.7 4.5 2M 2.1 5.4 4.1 6.2 6.9 7.2 3.7 5.6 4.8 5.7 6.1 4.7 6.5 8.6 6.5 6.7 6.5 6.3 4.0 5.5 5.7 3M 4.2 7.0 6.5 7.5 8.1 8.3 5.9 7.3 8.8 - 7.7 6.8 7.9 9.4 8.3 8.1 8.4 7.5 6.5 6.6 7.4

不再顯眼（10）]） ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS平均值 0M 0.3 5.0 2.7 4.8 4.2 4.5 3.4 7.0 3.5 4.9 2.5 0.0 4.8 8.3 5.0 8.0 5.4 5.3 0.9 2.8 4.2 1M 1.1 5.0 2.5 5.3 5.2 4.5 3.4 5.3 3.4 4.4 3.4 4.8 5.5 8.9 5.4 8.1 5.5 5.1 2.0 3.9 4.6 2M 1.8 5.7 4.4 6.7 7.1 6.9 4.8 6.2 4.8 5.7 3.9 5.5 6.2 8.8 6.7 7.8 6.5 6.5 3.1 4.9 5.7 3M 4.2 7.6 6.9 8.0 8.5 8.5 6.5 7.6 8.7 - 6.3 6.9 7.8 9.2 8.5 8.7 8.6 7.6 4.9 6.2 7.4 Regarding the conspicuous gray hair, a questionnaire survey of the subjects using VAS was conducted. In VAS, the evaluation value when gray hair becomes conspicuous is set to 0, and the evaluation value when gray hair is no longer conspicuous is set to 10. The results, as shown in Fig. 12 and Table 8, indicate that the gray hair is no longer conspicuous after starting to use the fragrance of the example. Moreover, as shown in Fig. 13, it is shown that the conspicuous gray hair has improved after starting to use the fragrance of the example. [Table 8] A table showing the transition of the conspicuous white hair of the subject obtained by the questionnaire survey of the embodiment (white hair [becomes conspicuous (0)

No longer conspicuous (10)]) ID E-01 E-02 E-03 E-04 E-05 E-06 E-07 E-08 E-09 E-10 E-11 E-12 E-13 E-14 E-15 E-16 E-17 E-18 E-19 E-20 VAS average 0M 0.3 5.0 2.7 4.8 4.2 4.5 3.4 7.0 3.5 4.9 2.5 0.0 4.8 8.3 5.0 8.0 5.4 5.3 0.9 2.8 4.2 1M 1.1 5.0 2.5 5.3 5.2 4.5 3.4 5.3 3.4 4.4 3.4 4.8 5.5 8.9 5.4 8.1 5.5 5.1 2.0 3.9 4.6 2M 1.8 5.7 4.4 6.7 7.1 6.9 4.8 6.2 4.8 5.7 3.9 5.5 6.2 8.8 6.7 7.8 6.5 6.5 3.1 4.9 5.7 3M 4.2 7.6 6.9 8.0 8.5 8.5 6.5 7.6 8.7 - 6.3 6.9 7.8 9.2 8.5 8.7 8.6 7.6 4.9 6.2 7.4

The doctor confirmed to the subject that the scalp was itchy and abnormal. As a result, as shown in Table 9, the state of the scalp was not confirmed. [Table 9] A table showing the diagnosis results of the physician and the hair diagnostician of the embodiment on the subject Itchy scalp Scalp abnormalities Scalp abnormalities caused by the test product ID 0M 1M 2M 3M 0M 1M 2M 3M 0M 1M 2M 3M E-01 no no no no no no no no - no no no E-02 no no no no no no no no - no no no E-03 no no no no no no no no - no no no E-04 no no no no no no no no - no no no E-05 no no no no no no no no - no no no E-06 no no no no no no no no - no no no E-07 no no no no no no no no - no no no E-08 no no no no no no no no - no no no E-09 no no no no no no no no - no no no E-10 no no no - no no no - - no no - E-11 no no no no no no no no - no no no E-12 no no no no no no no no - no no no E-13 no no no no no no no no - no no no E-14 no no no no no no no no - no no no E-15 no no no no no no no no - no no no E-16 no no no no no no no no - no no no E-17 no no no no no no no no - no no no E-18 no no no no no no no no - no no no E-19 no no no no no no no no - no no no E-20 no no no no no no no no - no no no Number of cases (people) Have 0 0 0 0 0 0 0 0 0 0 0 0 no 20 20 20 19 20 20 20 19 0 20 20 19 ratio(%) Have 0 0 0 0 0 0 0 0 0 0 0 0 no 100 100 100 100 100 100 100 100 0 100 100 100 M = months later

(Comparative example) Prepare the fragrance (beauty lotion) of the comparative example, which contains 0.001% by weight of palmitoyltetrapeptide-20, but does not contain acetotetrapeptide-3, red clover flower extract, trifluoroacetyl tripeptide-2, and apple fruit Cultivation stem cell extract, Mengzong bamboo growing point stem cell lysate and Willow flower/leaf/stem extract, and other compositions are the same as the flavor of the embodiment.

Twenty adults (average age 44) between 20 and 69 years old are selected as subjects. Before the subject started using the fragrance of the comparative example, the doctor and the hair diagnostician observed the gray hair on the top and side of the subject's head. In addition, doctors and hair diagnosticians use microscopes to observe the hair color at the roots of the subject's head (the hairline at the top of the helix).

Thereafter, the subjects (one subject did not conduct the test due to personal reasons) used shampoo containing antioxidants once a day for 3 months to wash their hair, and applied the fragrance of the comparative example to the entire scalp. During this period, the subjects were prohibited from using other shampoos, hair cosmetics, white hair dyes and hair dyes. After starting to use the fragrance of the comparative example, the doctor and the hair diagnostician observed the subject's hair in the first month, the second month, and the third month.

Fig. 14 and Table 10 show the transition of the degree of improvement in the amount of gray hair of the subject observed by the doctor and the hair diagnostician. The observation criteria are the same as the examples. Regarding the amount of gray hair on the top of the head, compared with before use, one month after the start of use, "unchanged" was 6 out of 19 (31%), and "slightly improved" was 10 out of 19 (53%). , "Improved" is 3 out of 19 (16%). Regarding the amount of gray hair on the top of the head, compared with before use, after 2 months of use, "unchanged" was 6 of 19 (32%), and "slightly improved" was 12 (63%) of 19 , "Improved" is 1 out of 19 (5%). Regarding the amount of gray hair on the top of the head, compared with before use, after 3 months of use, "slightly improved" was 13 of 19 (68%), and "improved" was 6 of 19 (32%). [Table 10] A table showing the transition of the rate of improvement in the amount of gray hair evaluated by the physician and the hair diagnostician of the comparative example Gray hair Top of head Side head ID 0M 1M 2M 3M 0M 1M 2M 3M C-01 - constant Slightly improved improve - constant Slightly improved Slightly improved C-02 - constant constant Slightly improved - constant constant Slightly improved C-03 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved C-04 - constant Slightly improved improve - constant Slightly improved Slightly improved C-05 - improve improve Slightly improved - Slightly improved constant Slightly improved C-06 - constant constant Slightly improved - constant Slightly improved Slightly improved C-07 - Slightly improved Slightly improved improve - Slightly improved Slightly improved Slightly improved C-08 - Slightly improved constant Slightly improved - Slightly improved constant Slightly improved C-09 - constant constant Slightly improved - constant Slightly improved Slightly improved C-10 - improve Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-11 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved improve C-12 - Slightly improved Slightly improved Slightly improved - improve Slightly improved Slightly improved C-13 - - - - - - - - C-14 - Slightly improved Slightly improved Slightly improved - constant Slightly improved Slightly improved C-15 - Slightly improved constant Slightly improved - Slightly improved Slightly improved improve C-16 - Slightly improved Slightly improved improve - Slightly improved Slightly improved Slightly improved C-17 - Slightly improved Slightly improved improve - Slightly improved Slightly improved Slightly improved C-18 - Slightly improved constant Slightly improved - Slightly improved Slightly improved Slightly improved C-19 - Slightly improved Slightly improved improve - Slightly improved improve improve C-20 - improve Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved n % n % n % n % n % n % Significant improvement - 0 0 0 0 0 0 - 0 0 0 0 0 0 improve - 3 16 1 5 6 32 - 1 5 1 5 3 16 Slightly improved - 10 53 12 63 13 68 - 11 58 15 79 16 84 constant - 6 31 6 32 0 0 - 7 37 3 16 0 0 deterioration - 0 0 0 0 0 0 - 0 0 0 0 0 0 M = months later

Therefore, in the case of using the fragrance of the comparative example, compared with the case of using the fragrance of the example, the degree of improvement in the amount of gray hair on the top of the head is lower.

Regarding the amount of gray hair on the lateral head, compared with before use, after 1 month of use, "unchanged" was 7 of 19 (37%), and "slightly improved" was 11 of 19 (58%) ), "improvement" is 1 out of 19 (5%). Regarding the amount of gray hair on the side head, compared with before use, after 2 months of use, "unchanged" was 3 out of 19 (16%), and "slightly improved" was 15 out of 19 (79%) ), "improvement" is 1 out of 19 (5%). Regarding the amount of gray hair on the side of the head, compared with before use, after 3 months of use, "slightly improved" was 16 out of 19 (84%), and "improved" was 3 out of 19 (16%). .

Therefore, in the case of using the fragrance of the comparative example, compared with the case of using the fragrance of the example, the degree of improvement in the amount of gray hair on the side head is lower.

Fig. 15 and Table 11 show the transition of the degree of improvement of the subject’s white hair color observed by the physician and the hair diagnostician. The observation criteria are the same as the examples. Regarding the white hair color on the top of the head, compared with before use, one month after the start of use, "unchanged" was 8 of 19 (42%), and "slightly improved" was 9 of 19 (47%). , "Improved" is 2 out of 19 (11%). Regarding the white hair color on the top of the head, compared with before use, after 2 months of use, "unchanged" was 5 out of 19 (26%), and "slightly improved" was 12 out of 19 (63%). , "Improved" is 2 out of 19 (11%). Regarding the white hair color on the top of the head, compared with before use, after 3 months of use, "slightly improved" was 12 of 19 (63%), and "improved" was 7 of 19 (37%). [Table 11] A table showing the transition of the gray hair color improvement rate evaluated by the physician and the hair diagnostician of the comparative example Gray hair color Top of head Side head ID 0M 1M 2M 3M 0M 1M 2M 3M C-01 - constant Slightly improved improve - constant Slightly improved Slightly improved C-02 - constant Slightly improved improve - constant Slightly improved Slightly improved C-03 - constant Slightly improved improve - constant Slightly improved Slightly improved C-04 - constant improve improve - constant Slightly improved improve C-05 - improve Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-06 - constant constant Slightly improved - constant constant constant C-07 - Slightly improved Slightly improved improve - Slightly improved Slightly improved improve C-08 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-09 - constant constant Slightly improved - constant constant Slightly improved C-10 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-11 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-12 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved improve C-13 - - - - - - - - C-14 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved C-15 - Slightly improved constant Slightly improved - Slightly improved constant Slightly improved C-16 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-17 - constant constant Slightly improved - constant Slightly improved Slightly improved C-18 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-19 - Slightly improved improve improve - Slightly improved Slightly improved improve C-20 - improve constant improve - Slightly improved constant Slightly improved n % n % n % n % n % n % Significant improvement - 0 0 0 0 0 0 - 0 0 0 0 0 0 improve - 2 11 2 11 7 37 - 0 0 0 0 4 twenty one Slightly improved - 9 47 12 63 12 63 - 11 58 15 79 14 74 constant - 8 42 5 26 0 0 - 8 42 4 twenty one 1 5 deterioration - 0 0 0 0 0 0 - 0 0 0 0 0 0 M = months later

Therefore, in the case of using the fragrance of the comparative example, compared with the case of using the fragrance of the example, the degree of improvement of the white hair color on the top of the head is lower.

Regarding the white hair color on the side of the head, compared with before use, after 1 month of use, "unchanged" was 8 of 19 (42%), and "slightly improved" was 11 of 19 (58%) ). Regarding the white hair color on the side of the head, compared with before use, after 2 months of use, "unchanged" was 4 of 19 (21%), and "slightly improved" was 15 of 19 (79%) ). Regarding the white hair color on the side of the head, compared with before use, after 3 months of use, "unchanged" was 1 of 19 (5%), and "slightly improved" was 14 of 19 (74%) ), "Improved" is 4 out of 19 (21%).

Therefore, in the case of using the fragrance of the comparative example, the degree of improvement in the white hair color of the side head is lower than the case of using the fragrance of the example.

Fig. 16 and Table 12 show the progress of the improvement rate of the white hair roots of the subjects observed by the doctor and the hair diagnostician. The observation criteria are the same as the examples. Regarding the condition of the white hair roots on the top of the head, compared with before use, one month after the start of use, "unchanged" was 8 of 19 (42%), and "slightly improved" was 11 of 19 (58) %). Regarding the condition of the white hair roots on the top of the head, compared with before use, after 2 months of use, the "unchanged" was 8 out of 19 (42%), and "slightly improved" was 11 out of 19 (58 %). Regarding the condition of the white hair roots on the top of the head, compared with before use, after 3 months of use, "unchanged" was 1 out of 19 (5%), and "slightly improved" was 13 out of 19 (69 %), "improvement" is 5 out of 19 (26%). [Table 12] A table showing the transition of the rate of improvement of the gray hair roots evaluated by the physicians and hair diagnosticians of the comparative example The state of white hair roots Top of head Side head ID 0M 1M 2M 3M 0M 1M 2M 3M C-01 - constant Slightly improved Slightly improved - constant improve improve C-02 - constant Slightly improved Slightly improved - constant improve improve C-03 - constant Slightly improved improve - constant Slightly improved Slightly improved C-04 - constant Slightly improved improve - constant improve improve C-05 - Slightly improved constant Slightly improved - Slightly improved Slightly improved Slightly improved C-06 - constant Slightly improved Slightly improved - constant Slightly improved Slightly improved C-07 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved improve C-08 - Slightly improved constant constant - Slightly improved Slightly improved Slightly improved C-09 - constant constant Slightly improved - constant constant Slightly improved C-10 - Slightly improved constant Slightly improved - improve constant Slightly improved C-11 - Slightly improved Slightly improved improve - Slightly improved Slightly improved Slightly improved C-12 - Slightly improved constant Slightly improved - Slightly improved Slightly improved Slightly improved C-13 - - - - - - - - C-14 - constant constant Slightly improved - constant Slightly improved improve C-15 - Slightly improved constant Slightly improved - Slightly improved Slightly improved improve C-16 - Slightly improved constant Slightly improved - Slightly improved constant Slightly improved C-17 - constant Slightly improved improve - constant improve improve C-18 - Slightly improved Slightly improved Slightly improved - Slightly improved Slightly improved Slightly improved C-19 - Slightly improved Slightly improved Slightly improved - Slightly improved improve improve C-20 - Slightly improved Slightly improved improve - improve improve improve n % n % n % n % n % n % Significant improvement - 0 0 0 0 0 0 - 0 0 0 0 0 0 improve - 0 0 0 0 5 26 - 2 11 6 31 9 47 Slightly improved - 11 58 11 58 13 69 - 9 47 10 53 10 53 constant - 8 42 8 42 1 5 - 8 42 3 16 0 0 deterioration - 0 0 0 0 0 0 - 0 0 0 0 0 0 M = months later

Therefore, in the case of using the fragrance of the comparative example, the degree of improvement of the white hair root on the top of the head is lower than that of the fragrance of the example.

Regarding the condition of the white hair roots on the side head, compared with before use, one month after the start of use, "unchanged" was 8 of 19 (42%), and "slightly improved" was 9 of 19 ( 47%), "Improved" is 2 out of 19 (11%). Regarding the condition of the white hair roots on the lateral head, compared with before use, after 2 months of use, "unchanged" was 3 out of 19 (16%), and "slightly improved" was 10 out of 19 ( 53%), "Improved" is 6 out of 19 (31%). Regarding the condition of the white hair roots on the lateral head, compared with before use, after 3 months of use, "slightly improved" was 10 out of 19 (53%), and "improved" was 9 out of 19 (47 %).

Therefore, in the case of using the fragrance of the comparative example, compared with the case of using the fragrance of the example, the degree of improvement of the white hair roots on the side head is lower.

no

Fig. 1 is a graph showing the transition of the rate of improvement in the amount of gray hair evaluated by the physician and the hair diagnostician of the embodiment. Fig. 2 is a graph showing the transition of the gray hair color improvement rate evaluated by the physician and the hair diagnostician of the embodiment. Fig. 3 is a graph showing the transition of the rate of improvement of the gray hair roots evaluated by the physicians and hair diagnosticians of the examples. FIG. 4 is a diagram showing the transition of the subject's worry about gray hair obtained by the questionnaire survey of the embodiment. Fig. 5 is a graph showing the transition of the VAS change rate when the subject is worried about gray hair obtained by the questionnaire survey of the embodiment. Fig. 6 is a graph showing the transition of the amount of gray hair of the subject obtained by the questionnaire survey of the embodiment. Fig. 7 is a graph showing the transition of the VAS change rate of the gray hair amount of the subject obtained by the questionnaire survey of the embodiment. Fig. 8 is a diagram showing the transition of the white hair color of the subject obtained by the questionnaire survey of the embodiment. Fig. 9 is a graph showing the transition of the VAS change rate of the white hair color of the subject obtained by the questionnaire survey of the embodiment. Fig. 10 is a diagram showing the transition of the gray hair range of the subject obtained by the questionnaire survey of the embodiment. FIG. 11 is a graph showing the transition of the VAS change rate of the gray hair range of the subject obtained by the questionnaire survey of the embodiment. Fig. 12 is a diagram showing the transition of the conspicuous white hair of the subject obtained by the questionnaire survey of the embodiment. FIG. 13 is a graph showing the transition of the VAS change rate of the subject's gray hair conspicuously obtained by the questionnaire survey of the embodiment. Fig. 14 is a graph showing the transition of the rate of improvement in the amount of gray hair evaluated by a physician and a hair diagnostician in a comparative example. Fig. 15 is a graph showing the transition of the gray hair color improvement ratio evaluated by the physician and the hair diagnostician of the comparative example. Fig. 16 is a graph showing the transition of the rate of improvement of the gray hair roots evaluated by the doctor and the hair diagnostician of the comparative example.

Claims (21)

A hair treatment composition, comprising: 1.0% by weight or more Greyverse (registered trademark); 2.5% by weight or more Capixyl (registered trademark); 0.05% by weight or more Progeline (registered trademark); 0.009% by weight or more of apple stem cell lysis Material; Bamboo stem cell lysate of 0.00625% by weight or more; and Defenscalp (registered trademark) of 0.1% by weight or more. The composition according to claim 1, which prevents gray hair or gray hair. The composition according to claim 1 or 2, which increases the melanin of hair. The composition according to claim 1 or 2, which is a shampoo. The composition according to claim 1 or 2, which is a beauty liquid. A hair treatment composition comprising: more than 0.0005 wt% of palmitoyltetrapeptide-20 or palmitoyltetrapeptide-20 amide; more than 0.00069 wt% of acetyltetrapeptide-3; more than 0.00001 wt% of trifluoroethylene Tripeptide-2; a stem cell lysate of 0.01525 wt% or more; and an anti-inflammatory plant extract of 0.00563 wt% or more, the stem cell lysate includes apple stem cell lysate, and the anti-inflammatory plant extract includes a Willow orchid extract. The composition according to claim 6, which contains 0.009% by weight or more of the above-mentioned apple stem cell lysate. A hair treatment composition comprising: more than 0.0005% by weight of palmitoyl tetrapeptide-20 or palmitoyl tetrapeptide-20 amide; 0.00069% by weight or more of acetyltetrapeptide-3; 0.00001% by weight or more of trifluoroacetate tripeptide-2; 0.01525% by weight or more of stem cell lysate; and 0.00563% by weight or more of anti-inflammatory plant extracts, the above-mentioned stem cell lysis The extract includes bamboo stem cell lysate, and the anti-inflammatory plant extract includes Liulan extract. The composition according to claim 6 or 8, which contains 0.005% by weight or more of the above-mentioned Liulan extract. The composition according to claim 6 or 8, wherein the Liulan extract is contained in Defenscalp (registered trademark). A hair treatment composition comprising: more than 0.0005 wt% of palmitoyltetrapeptide-20 or palmitoyltetrapeptide-20 amide; more than 0.00069 wt% of acetyltetrapeptide-3; more than 0.00001 wt% of trifluoroethylene Tripeptide-2; stem cell lysate of more than 0.01525 wt%; and anti-inflammatory plant extract of more than 0.00563 wt%, said stem cell lysate contains bamboo stem cell lysate, and said anti-inflammatory plant extract contains red clover flower extract Things. The composition according to claim 8 or 11, which contains 0.00625% by weight or more of the above-mentioned bamboo stem cell lysate. The composition according to claim 11, which contains more than 0.00063% by weight of the red clover flower extract. The composition according to claim 11 or 13, wherein the red clover flower extract is contained in Capixyl (registered trademark). The composition according to any one of claims 6 to 8, 11, and 13, wherein the palmitoyl tetrapeptide-20 amide is contained in Greyverse (registered trademark). The composition according to any one of claims 6 to 8, 11, and 13, wherein the acetotetrapeptide-3 is contained in Capixyl (registered trademark). The composition according to any one of claims 6 to 8, 11, and 13, wherein the above-mentioned trifluoroacetyl tripeptide-2 is contained in Progeline (registered trademark). The composition according to any one of claims 6 to 8, 11, and 13, which prevents gray hair or gray hair. The composition according to any one of claims 6 to 8, 11, and 13, which increases the melanin of hair. The composition according to any one of claims 6 to 8, 11, and 13, which is a shampoo. The composition according to any one of claims 6 to 8, 11, and 13, which is a beauty liquid.

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