CN116172935A

CN116172935A - Use for producing a hair care composition for preventing white or grey hair

Info

Publication number: CN116172935A
Application number: CN202211719514.3A
Authority: CN
Inventors: 大宫理成
Original assignee: Nature Research Co ltd
Current assignee: Nature Research Co ltd
Priority date: 2019-02-20
Filing date: 2019-08-13
Publication date: 2023-05-30
Also published as: TW202031244A; TWI706790B; WO2020170476A1; TW202031243A; SG11202108852PA; JP6556392B1; CN113645948A; CN114432177A; JP2020132577A; TWI821493B; MY192292A

Abstract

The present invention provides a use for the manufacture of a hair care composition for preventing white or grey hair, the composition comprising: 1.0% by weight or more of Greyverse registered trademark, 2.5% by weight or more of Capixyl registered trademark, 0.05% by weight or more of Progeline registered trademark, 0.009% by weight or more of apple stem cell lysate, 0.00625% by weight or more of bamboo stem cell lysate, and 0.1% by weight or more of Defenscalp registered trademark.

Description

Use for producing a hair care composition for preventing white or grey hair

The present application is a divisional application of the invention patent application with international application number PCT/JP2019/031876, international application date 2019, 8 months 13, and application number 201980092609.7, named "hair care composition", entering the national stage of china.

Technical Field

The present invention relates to the use for the manufacture of a hair care composition for the prevention of white or grey hair.

Background

Hair color is formed by melanin. Among melanin, there are 2 kinds of eumelanin from brown to black and brownish melanin from yellow to red. The hair color is determined by the ratio of eumelanin to eumelanin in the hair. For example, when there is a lot of eumelanin and less brown melanin, the hair becomes black. In the case of less eumelanin and more brownish melanin, the hair becomes gold or red. When melanin contained in hair is reduced or eliminated, the hair becomes white or grey hair (for example, see patent documents 1 to 3).

Pigment stem cells are present in the convex region of the hair follicle. Pigment stem cells produce pigment cells (melanocytes). Pigment cells produce melanin and supply hair mother cells (keratinocytes). The hair mother cells supplied with melanin divide into hair and grow. Pigment stem cells are thought to decrease or decrease in activity with age. The decrease in pigment stem cells and the decrease in activity are considered to be the cause of white hair or grey hair.

Prior art literature

Patent literature

Patent document 1 japanese patent No. 3289975

Patent document 2 japanese patent No. 4971072

Patent document 3 japanese patent No. 5850266

Disclosure of Invention

Problems to be solved by the invention

It is an object of the present invention to provide a use for manufacturing a hair care composition for preventing white or grey hair, which hair care composition can effectively increase melanin of hair.

Means for solving the problems

According to an embodiment of the present invention, there is provided a hair care composition comprising: 0.0005 wt% or more of melanin synthesis-promoting peptide, 0.00069 wt% or more of hair-regenerating peptide, 0.00001 wt% or more of anti-aging peptide, 0.01525 wt% or more of stem cell lysate, and 0.00563 wt% or more of anti-inflammatory plant extract.

In the above hair care composition, the melanin synthesis-promoting peptide may be palmitoyl tetrapeptide-20 or palmitoyl tetrapeptide-20 amide.

In the above hair care composition, palmitoyl tetrapeptide-20 amide may be contained in Greyverse (registered trademark).

In the above hair care composition, the hair regenerating peptide may be acetyl tetrapeptide-3.

In the above hair care composition, the hair-regrowing peptide may be contained in Cap i xy l (registered trademark).

In the hair care composition described above, the anti-aging peptide may be trifluoroacetyl tripeptide-2.

In the above hair care composition, the anti-aging peptide may be contained in progel i ne (registered trademark).

In the hair care composition described above, the stem cell lysate may comprise apple stem cell lysate.

The hair care composition may contain 0.009 wt% or more of apple stem cell lysate.

In the hair care composition described above, the stem cell lysate may comprise bamboo stem cell lysate.

The hair care composition may contain 0.00625 wt% or more of bamboo stem cell lysate.

In the above hair care composition, the anti-inflammatory plant extract may comprise a willow herb extract.

The hair care composition may contain the willow herb extract in an amount of 0.005 wt% or more.

In the above hair care composition, the willow herb extract may be contained in the safenca l p (registered trademark).

In the above hair care composition, the anti-inflammatory plant extract may comprise an extract of flowers of red clover.

The hair care composition may contain an extract of flowers of red clover in an amount of 0.00063 wt% or more.

In the above hair care composition, the flower extract of red clover may be contained in Cap i xy l (registered trademark).

The hair care composition can prevent white hair or grey hair.

The hair care composition can increase melanin of hair.

The hair care composition may be a shampoo.

The hair care composition may be a cosmetic liquid.

According to an embodiment of the present invention, there is provided a method of preventing white or grey hair, the method comprising applying the hair care composition described above to the scalp.

According to an embodiment of the present invention, there is provided a method of increasing melanin of hair, the method comprising applying the hair care composition described above to the scalp.

According to an embodiment of the present invention, there is provided a hair care composition comprising: 1.0% by weight or more of Greyverse (registered trademark), 2.5% by weight or more of Cap i xy l (registered trademark), 0.05% by weight or more of Proge i ne (registered trademark), 0.009% by weight or more of apple stem cell lysate, 0.00625% by weight or more of bamboo stem cell lysate, and 0.1% by weight or more of Defensca l p (registered trademark).

The hair care composition can prevent white hair or grey hair.

The hair care composition can increase melanin of hair.

The hair care composition may be a shampoo.

The hair care composition may be a cosmetic liquid.

ADVANTAGEOUS EFFECTS OF INVENTION

The present invention may provide a use for manufacturing a hair care composition for preventing white or grey hair, which hair care composition may be effective in increasing melanin of hair.

Drawings

Fig. 1 is a graph showing the evolution of the improvement ratio of the white hair volume evaluated by the doctor and the hair diagnostician according to the example.

Fig. 2 is an evolution table showing the improvement ratio of the white hair amount evaluated by the doctor and the hair diagnostician according to the example.

Fig. 3 is a graph showing the evolution of the improvement ratio of white hair color evaluated by the doctor and the hair diagnostician according to the example.

Fig. 4 is an evolution table showing the improvement ratios of white hair color evaluated by physicians and hair diagnosticians according to the examples.

Fig. 5 is a graph showing the evolution of the improvement ratio of the white hair root evaluated by the doctor and the hair diagnostician according to the example.

Fig. 6 is an evolution table showing the improvement ratio of the white hair root evaluated by the doctor and the hair diagnostician according to the example.

Fig. 7 is a graph and table showing the evolution of the attention status of the white hair by the questionnaire of the experimental participants according to the examples.

Fig. 8 is a graph and table showing the evolution of the VAS change rate of the deliberate condition of the white hair obtained by the questionnaire of the experimental participants according to the example.

Fig. 9 is a graph and table showing the evolution of the amount of white hair reflected by a questionnaire of the experimental participants according to the examples.

Fig. 10 is a graph and table showing the evolution of the VAS change rate of the white hair amount obtained by the questionnaire of the experimental participants according to the example.

Fig. 11 is a graph and table showing the evolution of white hair color obtained from a questionnaire of an experimental participant according to the example.

Fig. 12 is a graph and table showing the evolution of the VAS change rate of the white hair color obtained by the questionnaire of the panelist according to the example.

Fig. 13 is a graph and table showing the evolution of the white hair range obtained by the questionnaire of the experimental participants according to the examples.

Fig. 14 is a graph and table showing the evolution of the VAS change rate in the white hair range obtained by the questionnaire of the experimental participants according to the example.

Fig. 15 is a graph and table showing the development of the conspicuity of the white hair obtained by the questionnaire of the experimental participants according to the examples.

Fig. 16 is a graph and table showing the evolution of the VAS change rate of the conspicuous degree of the white hair obtained by the questionnaire of the experimental participants according to the example.

Fig. 17 is a table showing the results of diagnosis of the experimental participants by the doctor and the hair diagnostician according to the example.

Fig. 18 is a graph showing the evolution of the improvement ratio of the white hair amount evaluated by the doctor and the hair diagnostician according to the comparative example.

Fig. 19 is a table showing the evolution of the improvement degree ratio of the white hair amount evaluated by the doctor and the hair diagnostician according to the comparative example.

Fig. 20 is a graph showing the evolution of the improvement ratio of white hair color evaluated by a doctor and a hair diagnostician according to the comparative example.

Fig. 21 is a table showing the evolution of the improvement ratio of the white hair color evaluated by the doctor and the hair diagnostician according to the comparative example.

Fig. 22 is a graph showing the evolution of the improvement ratio of the white hair root evaluated by the doctor and the hair diagnostician according to the comparative example.

Fig. 23 is a table showing the evolution of the improvement ratio of the white hair root evaluated by the doctor and the hair diagnostician according to the comparative example.

Detailed Description

Embodiments of the present invention are described in detail below. The embodiments described below are examples of an apparatus and a method for embodying the technical idea of the present invention, and combinations of constituent elements and the like in the technical idea of the present invention are not limited to the following. The technical idea of the present invention can be variously modified within the scope of patent application protection.

The hair care composition according to the embodiment comprises: 0.0005 wt% or more of melanin synthesis-promoting peptide, 0.00069 wt% or more of hair-regenerating peptide, 0.00001 wt% or more of anti-aging peptide, 0.01525 wt% or more of stem cell lysate, and 0.00563 wt% or more of anti-inflammatory plant extract.

The concentration of the melanin synthesis-promoting peptide in the hair care composition according to the embodiment may be 0.0005 wt% or more, 0.00065 wt% or more, or 0.0008 wt% or more. The concentration of the melanin synthesis-promoting peptide may be 0.0025 wt% or less, 0.002 wt% or less, or 0.0015 wt% or less. Melanin synthesis-promoting peptides promote melanin synthesis. The melanin synthesis-promoting peptide binds to, for example, melanocortin receptor 1 (MC 1-R). In addition, the melanin synthesis-promoting peptide can also reduce the oxidative stress of melanin that causes the generation of grey hair or grey hair. For example, the melanin synthesis-promoting peptide activates deacetylase (S I RT) as a long-acting protein and activates tyrosine kinase-related protein (TRP) -2 as an antioxidant enzyme.

Examples of the melanin synthesis-promoting peptide include: palmitoyl tetrapeptide-20, palmitoyl tetrapeptide-20 amide, and acetyl hexapeptide-1. Palmitoyl tetrapeptide-20 is the reaction product of palmitic acid and a synthetic tetrapeptide composed of arginine, histidine, phenylalanine, and tryptophane. Palmitoyl tetrapeptide-20 is a biomimetic peptide of alpha-Melanocyte Stimulating Hormone (MSH). Palmitoyl tetrapeptide-20 amide may be contained in Greyverse (registered trademark of AH & NS).

The concentration of Greyverse (registered trademark of AH & NS corporation) in the hair care composition according to the embodiment may be 1.0 wt% or more, 1.25 wt% or more, or 1.5 wt% or more. The concentration of Greyverse (registered trademark of AH & NS corporation) may be 6.0 wt% or less, 5.5 wt% or less, or 5.0 wt% or less.

The concentration of the hair-regenerating peptide in the hair care composition according to the embodiment may be 0.00069 wt% or more, 0.0009 wt% or more, or 0.0011 wt% or more. The concentration of the hair-regenerating peptide may be 0.00345 wt% or less, 0.00276 wt% or less, or 0.00207 wt% or less. The hair regenerating peptide may be a peptide that protects hair follicles and hair roots. In addition, the hair-regenerating peptide may have an anti-inflammatory effect of inhibiting inflammation leading to the generation of grey or grey hair. The hair regenerating peptide may be a peptide that inhibits 5α reductase and inhibits androgens.

Examples of the hair-regenerating peptide include acetyl tetrapeptide-3 and tripeptide-1 copper. Acetyl tetrapeptide-3 is obtained by acetylating synthesized tetrapeptides including lysine, glycine, histidine and lysine. Acetyl tetrapeptide-3 may be contained in Cap i xy l (registered trademark of AH & NS corporation).

The concentration of Cap i xy l (registered trademark of AH & NS corporation) in the hair care composition according to the embodiment may be 2.5 wt% or more, 3.125 wt% or more, or 3.75 wt% or more. The concentration of Cap i xy l (registered trademark of AH & NS corporation) may be 15.0 wt% or less, 13.75 wt% or less, or 12.5 wt% or less.

The concentration of the anti-aging peptide in the hair care composition according to the embodiment may be 0.00001 wt% or more, or 0.00002 wt% or more. The concentration of the anti-aging peptide may be 0.00005 wt% or less, 0.00004 wt% or less, or 0.00003 wt% or less. Anti-aging peptides, for example, increase the tolerance of hair follicle cells and hair papilla cells to stress hormones. The anti-aging peptide may have a function of inhibiting inflammation that causes the generation of grey hair or grey hair. The presence of the hair-regenerating peptide and the anti-aging peptide tends to inhibit neutrophil elastase and protect hair follicles from stress hormone.

As an example of the anti-aging peptide, trifluoroacetyl tripeptide-2 may be mentioned. Trifluoroacetyl tripeptide-2 may be obtained from valinic acid, tyrosine, tripeptide-2 comprising valinic acid and trifluoroacetic acid. Trifluoroacetyl tripeptide-2 may be contained in progel i ne (registered trademark of AH & NS company).

The concentration of progel i ne (registered trademark of AH & NS corporation) in the hair care composition according to the embodiment may be 0.05 wt% or more, 0.0625 wt% or more, or 0.075 wt% or more. The concentration of progel i ne (registered trademark of AH & NS corporation) may be 0.3 wt% or less, 0.275 wt% or less, or 0.25 wt% or less.

The concentration of stem cell lysate in the hair care composition according to the embodiment may be 0.01525 wt% or more, 0.01976 wt% or more, or 0.02432 wt% or more. The concentration of the stem cell lysate may be 0.076 wt% or less, 0.061 wt% or less, or 0.0456 wt% or less. The stem cell lysate may be an extract of stem cells. The stem cell lysate comprises at least a lysate of cytoplasmic components of the stem cells. The stem cell lysate may contain a lysate of a cell wall component of stem cells in addition to a lysate of a cytoplasmic component of stem cells.

The stem cell lysate may comprise apple stem cell lysate. The apple stem cell lysate may be an extract of apple stem cells. Apple stem cells have the effect of maintaining melanin in the hair roots. Apple stem cell lysate comprises at least a lysate of cytoplasmic components of apple stem cells. The apple stem cell lysate may also comprise a lysate of a cell wall component of apple stem cells in addition to a lysate of a cytoplasmic component of apple stem cells. Apple stem cell lysate, for example, protects the scalp, protects hair follicles, activates raised stem cells. The presence of hair-regenerating peptides and apple stem cell lysate tends to enhance the hair root protecting effect and the collagen 17 production promoting effect. The method for producing the apple stem cell lysate is any method, and for example, the apple stem cell lysate can be obtained by applying high pressure to apple stem cells. Apple stem cell lysate may be contained in a solvent containing lecithin, glycerol, xanthan gum and phenoxyethanol.

The concentration of apple stem cell lysate in the hair care composition according to the embodiment may be 0.009% by weight or more, 0.0117% by weight or more, or 0.0144% by weight or more. In addition, the concentration of apple stem cell lysate may be 0.045 wt% or less, 0.036 wt% or less, or 0.027 wt% or less. As apple stem cell lysate, for example, phytoCe l l Tec Ma l us Domest i ca (registered trademark of AH & NS company) can be used.

The stem cell lysate may comprise bamboo stem cell lysate. The bamboo stem cell lysate may be an extract of bamboo stem cells. As the bamboo stem cells, the stem cells of the growth point of the phyllostachys praecox can be used. The bamboo stem cell lysate comprises at least a lysate of cytoplasmic components of bamboo stem cells. The bamboo stem cell lysate may contain a lysate of a cell wall component of bamboo stem cells in addition to a lysate of a cytoplasmic component of bamboo stem cells. The method for producing the bamboo stem cell lysate is any method, and for example, an aqueous solution of cytoplasm of bamboo stem cells can be extracted.

The concentration of bamboo stem cell lysate in the hair care composition according to the embodiment may be 0.00625 wt% or more, 0.00813 wt% or more, or 0.01 wt% or more. The concentration of the bamboo stem cell lysate may be 0.03125 wt% or less, 0.025 wt% or less, or 0.01875 wt% or less. Bamboo stem cell lysate has anti-glycation effect, and can promote collagen production and induce keratin. In addition, when the scalp is saccharified, the scalp tends to become hard. Bamboo stem cell lysate, hair regenerating peptide and apple stem cell lysate are present, and thus have a tendency to improve anti-glycation and keratin-inducing effects. Bamboo stem cell lysates are available, for example, from AH & NS company.

The concentration of the anti-inflammatory plant extract in the hair care composition according to the embodiment may be 0.00563 wt% or more, 0.00732 wt% or more, or 0.00901 wt% or more. The concentration of the anti-inflammatory plant extract may be 0.02815 wt% or less, 0.02252 wt% or less, or 0.01689 wt% or less. The anti-inflammatory plant extract can protect hair root. The presence of hair regenerating peptides and anti-inflammatory plant extracts tends to improve the anti-inflammatory effect and the hair root protecting effect.

As an example of the anti-inflammatory plant extract, willow herb extract is cited. The extract of Liriosa may be an extract of flowers of Liriosa, an extract of leaves of Liriosa and an extract of stems of Liriosa, and mixtures thereof. The willow herb extract maintains the amount of malassezia, which is a resident strain of the scalp, in a moderate state to protect the hair roots. The willow herb extract contains evening primrose extract B shown in the following chemical formula as an active ingredient.

[ chemical formula 1]

The concentration of the willow herb extract in the hair care composition according to the embodiment may be 0.005 wt% or more, 0.0065 wt% or more, or 0.008 wt% or more. The concentration of the willow herb extract in the hair care composition according to the embodiment may be 0.025 wt% or less, 0.02 wt% or less, or 0.015 wt% or less. The willow herb extract may be contained in Defensca l p (registered trademark of AH & NS company).

The concentration of the Defensca l p (registered trademark of AH & NS corporation) in the hair care composition according to the embodiment may be 0.1% by weight or more, 0.125% by weight or more, or 0.15% by weight or more. The concentration of Defensca l p (registered trademark of AH & NS corporation) may be 0.6 wt% or less, 0.55 wt% or less, or 0.5 wt% or less.

Further, as examples of the anti-inflammatory plant extract, there are flower extracts of red clover and avocado fatty acid butyl ester. In the hair care composition according to the embodiment, it is preferable that a flower extract of red clover or avocado fatty acid butyl ester is blended together with the hair-regenerating peptide. The red clover has the effect of inhibiting interleukin 8 which can cause injury to papilla cells. In addition, red clover contains biochanin a represented by the following chemical formula.

[ chemical formula 2]

The concentration of the red clover flower extract in the hair care composition according to the embodiment may be 0.00063 wt% or more, 0.00082 wt% or more, or 0.001 wt% or more. The concentration of the red clover flower extract may be 0.00315 wt% or less, 0.00232 wt% or less, or 0.00189 wt% or less. The flower extract of red clover may be contained in Cap i xy l (registered trademark of AH & NS company) or M i n i point l (registered trademark of AH & NS company).

The hair care composition according to the embodiment may be a shampoo, a skin care liquid, a conditioner, a gel, a hair care agent, a hair cream, a tincture, a spray, a mousse, a cleaning composition, a hair cream, a hair lotion, a hair cream, a foam, or the like. The hair care composition according to the embodiment is applied to the scalp 1 or more times per day, for example.

Hair care compositions according to embodiments may comprise, for example: water, water-soluble organic solvents such as C1-C4-alkanols, oils, fatty substances, waxes, esters of C6-C30 monocarboxylic acids with mono-, di-or triols, saturated acyclic and cyclic hydrocarbons, fatty acids, aliphatic alcohols, silicone oils, interface-active ingredients, defoamers, propellants, defoamers, emulsifiers, foaming agents, and solubilizers. The interfacial active ingredient may be anionic, cationic, amphoteric and neutral. Emulsifiers may be anionic, cationic, amphoteric and neutral.

The hair care composition according to the embodiment may comprise, for example, a water-soluble or water-dispersible hair polymer. Examples of the hair polymer include styling polymers and conditioning polymers. More specifically, examples of the hair polymer include: copolymers of vinyl acetate and butenoic acid, copolymers of methyl vinyl ether and anhydrous maleic anhydride, copolymers of acrylic or methacrylic acid and other monomers, polyurethane, N-vinylpyrrolidone and silicone polymers.

The hair care composition according to the embodiment may include, for example, a water-insoluble silicone such as polydimethylsiloxane. The hair care composition according to the embodiment may contain, for example, a wax such as fatty amide.

The hair care composition according to the embodiment may comprise an antioxidant, for example. The above willow herb extract and flower extract of Trifolium pratense can also act as antioxidant. Examples of other antioxidants include: heme, vitamin C and vitamin E.

The hair care composition according to the embodiment may contain, for example, a protectant, an antioxidant, hair oil, a lipid re-fatting agent, an active ingredient, and a care ingredient. For example, hair care compositions according to embodiments may include panthenol, collagen, vitamins, protein hydrolysates, alpha and beta hydroxy carbonic acid, stabilizers, pH adjusters, creamers, colorants, dyes, gel formers, salts, humectants, complexing agents, viscosity modifiers, and opacifiers.

The hair care compositions according to embodiments may comprise, for example, polyalkylsiloxanes, polyarylsiloxanes, polyarylalkylsiloxanes, polysilicone resins, polyether siloxanes, dimethicone Copolyols (CTFA) and amine functional polysilicone compounds.

In the case where the hair care composition according to the embodiment includes a propellant, the hair care composition according to the embodiment may include, for example: propane/butane, pentane, dimethyl ether, 1-difluoroethane (HFC-152 a), carbon dioxide, nitrogen and compressed air.

In the case where the hair care composition according to the embodiment includes a nonionic emulsifier, the hair care composition according to the embodiment may include, for example: laureth such as laureth-4, cetyl polyether such as cetyl polyether-1, polyethylene glycol cetyl ether, cetostearyl alcohol polyether such as cetostearyl alcohol polyether-25, polyglycol fatty acid glyceride, hydroxylated lecithin, lactoyl esters of fatty acids, and alkyl polyglycoside.

In the case where the hair care composition according to the embodiment includes a nonionic surfactant, the hair care composition according to the embodiment may include, for example: reaction products of aliphatic alcohols or alkylphenols having a linear or branched alkyl chain of 6 to 20 carbon atoms with ethylene oxide and/or propylene oxide.

Hair care compositions according to embodiments may comprise, for example: alkyl amine oxides, mono-or dialkyl alkanolamides, fatty acid esters of polyethylene glycols, alkyl polyglycosides and sorbitan esters.

When the hair care composition according to the embodiment includes a cationic emulsifier and/or a cationic surfactant, the hair care composition according to the embodiment may include, for example, a quaternary ammonium compound. Hair care compositions according to embodiments may comprise, for example: cetyltrimethylammonium chloride or bromide, stearyl benzyldimethylammonium chloride, distearyldimethylammonium chloride, stearamidopropyl dimethylamine, hydroxyethyl cetyldimethylammonium phosphate, poly (oxy-1, 2-ethanediyl), and (octadecyl-nitrogen) tris-2, 1- (ethanediyl) tris- (hydroxy) -phosphate. The hair care composition according to the embodiment may include, for example, a cationic guar derivative such as guar hydroxypropyl trimethylammonium chloride.

In the case where the hair care composition of the embodiment contains an anionic emulsifier and/or an anionic surfactant, the hair care composition according to the embodiment may contain, for example: alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkylaryl sulfonates, alkyl succinates, alkyl sulfosuccinates, N-alkyl creatine amine salts, acyl taurates, acyl isethionates, alkyl phosphates, alkyl ether carboxylates, and alpha-olefin sulfonates.

In the case where the hair care composition according to the embodiment includes an anionic surfactant, the hair care composition according to the embodiment may include, for example: sodium lauryl sulfate, ammonium lauryl sulfate, sodium lauryl ether sulfate, ammonium lauryl ether sulfate, sodium lauroyl sarcosinate, sodium oleyl succinate, ammonium laurylsulfosuccinate, sodium dodecylbenzenesulfonate, and triethanolamine decylbenzenesulfonate.

In the case where the hair care composition according to the embodiment includes an amphoteric surfactant, the hair care composition according to the embodiment may include, for example: alkyl betaines, alkyl amidopropyl betaines, alkyl sulfobetaines, alkyl glycinates, alkyl carboxyglycinates, alkyl amphoacetates or propionates, and alkyl amphodiacetates or dipropionates.

The hair care composition according to the embodiment can increase melanin of hair and prevent white hair or grey hair. The hair care composition according to the embodiment can restore the hair color to a color that turns into white hair or grey hair.

While not being bound by theory, by applying the hair care composition of the embodiments to the scalp, the melanin synthesis-promoting peptide promotes melanin synthesis in the hair follicle. Even if melanin synthesis is promoted, white or grey hair cannot be effectively restored to hair containing melanin without growing thick hair. In this regard, hair regenerating peptides and stem cell lysates promote thickening and lengthening of hair containing melanin. Furthermore, the anti-aging peptide increases the tolerance of hair follicle cells and hair papilla cells to stress hormones, and the anti-inflammatory plant extract suppresses inflammation in hair follicles, thereby effectively restoring white or gray hair to hair containing melanin.

The present invention has been described in the embodiments described above, but the description and drawings, which are part of the disclosure, should not be construed as limiting the invention. Various alternative implementations, examples, and techniques of use will be apparent to those skilled in the art from this disclosure. It should be understood that the present invention encompasses various embodiments and the like not described herein.

Example (example)

The essence (cosmetic liquid) according to the preparation example comprises: 0.001 wt% palmitoyl tetrapeptide-20, 0.00138 wt% acetyl tetrapeptide-3, 0.00125 wt% red clover extract, 0.00002 wt% trifluoroacetyl tripeptide-2, 0.018 wt% apple fruit culture stem cell extract, 0.0125 wt% Phyllostachys Pubescens growth point stem cell lysate, and 0.01 wt% Liu Lanhua/leaf/stem extract.

Palmitoyl tetrapeptide-20 is contained within Greyverse (registered trademark). The content of Greyverse (registered trademark) in the essence according to examples was 2.0 wt%. Acetyl tetrapeptide-3 and red clover extract are contained in Cap i xy l (registered trademark). The content of Cap i xy l (registered trademark) in the essence according to examples was 5.0 wt%. Trifluoroacetyl tripeptide-2 is contained in progel i ne (registered trademark). The content of progel i ne (registered trademark) in the essence according to examples was 0.1 wt%. Apple fruit culture stem cell extract and Phyllostachys pubescens growth point stem cell lysate were purchased from AH & NS company. Liu Lanhua the leaf/stem extract is contained in Defensca l p (registered trademark). The content of defloccuca l p (registered trademark) in the essence according to examples was 0.2 wt%.

20 adults 20 years of age 20 to 69 years of age (average age 43 years) were selected as test participants. Before the experimenter began to use the essence according to the examples, the doctor and the hair diagnostician observed the white hair on the top of the experimenter's head and on the lateral head. In addition, doctors and hair diagnosticians observe the hair color of the hair root of the lateral head (hairline at the top of the helix) of the experimenter using a microscope.

Thereafter, the panelists shampoo with 1 shampoo containing an antioxidant every day during 3 months, and the essence according to the examples was applied to the entire scalp. Meanwhile, the investigator is prohibited from using other shampoos, hair cosmetics, white hair dyes, and the like. After starting the use of the essence according to the examples, the physician and the hair diagnostician observe the experimenters' hair at month 1, month 2 and month 3. Furthermore, after 2 months from the start of the use of the essence according to the examples, 1 experimenter was no longer involved in the test for personal reasons.

The evolution of the improvement ratio of the white hair amount of the test subjects observed by the doctor and the hair diagnostician is schematically shown in fig. 1 and 2. Further, the improvement was evaluated in the case of a decrease in the amount of white hair. For the amount of white hair at the top of the head, 8 out of 20 (40%) were "unchanged", 5 out of 20 (25%) were "slightly improved", and 7 out of 20 (35%) were "improved" after 1 month of initial use, as compared to before use. For the amount of white hair at the top of the head, 5 out of 20 (25%) were "unchanged", 10 out of 20 (50%) were "slightly improved", and 5 out of 20 (25%) were "improved" after starting to use for 2 months as compared to before use. For the amount of white hair at the top of the head, 8 out of 19 (42%) were "slightly improved" and 11 out of 19 (58%) were "improved" after 3 months of initial use, as compared to before use.

For the white hair amount of the lateral head, 8 out of 20 (40%) were "unchanged", 5 out of 20 (25%) were "slightly improved", and 7 out of 20 (35%) were "improved" after 1 month of starting use, as compared with before use. For the white hair amount of the lateral head, 1 out of 20 (5%) was "unchanged", 16 out of 20 (80%) was "slightly improved", and 3 out of 20 (15%) was "improved" after starting to use for 2 months as compared with before use. For the white hair amount of the lateral head, 10 out of 19 (53%) were "slightly improved" and 9 out of 19 (47%) were "improved" after 3 months of initial use, as compared to before use.

The evolution of the improvement ratio of the white hair color of the experimenter, which is observed by the doctor and the hair diagnostician, is schematically shown in fig. 3 and 4. Further, the improvement was evaluated in the case of the white hair having a color. For the white hair color at the top of the head, 8 out of 20 (40%) were "unchanged", 7 out of 20 (35%) were "slightly improved", and 5 out of 20 (25%) were "improved" after 1 month of initial use, as compared to before use. For the white hair color at the top of the head, 2 out of 20 (10%) were "unchanged", 15 out of 20 (75%) were "slightly improved", and 3 out of 20 (15%) were "improved" after 2 months of initial use compared to before use. For the white hair color at the top of the head, 10 out of 19 (53%) were "slightly improved" and 9 out of 19 (47%) were "improved" after 3 months of initial use, as compared to before use.

For the white hair color of the lateral head, 8 out of 20 (40%) were "unchanged", 9 out of 20 (45%) were "slightly improved", and 3 out of 20 (15%) were "improved" after 1 month of initial use compared to before use. For the white hair color of the lateral head, 1 out of 20 (5%) was "unchanged", 17 out of 20 (85%) was "slightly improved", and 2 out of 20 (10%) was "improved" after starting to use for 2 months as compared to before use. For the white hair color of the lateral head, 13 out of 19 (68%) were "slightly improved" and 6 out of 19 (32%) were "improved" after 3 months of initial use, as compared to before use.

The evolution of the improvement ratio of the white hair root of the experimenter, which is observed by the doctor and the hair diagnostician, is schematically shown in fig. 5 and 6. In addition, the grey hair root was evaluated as improvement in the case of grey hair. For the state of the white hair root at the top of the head, 8 out of 20 (40%) were "unchanged", 5 out of 20 (25%) were "slightly improved", and 7 out of 20 (35%) were "improved" after 1 month of initial use, as compared with before use. For the state of the white hair root at the top of the head, 3 out of 20 (15%) were "unchanged", 14 out of 20 (70%) were "slightly improved", and 3 out of 20 (15%) were "improved" after starting to use for 2 months, as compared with before use. For the state of the white hair root at the top of the head, 10 out of 19 (53%) were "slightly improved" and 9 out of 19 (47%) were "improved" after 3 months of initial use, as compared to before use.

For the state of the white hair root of the lateral head, 9 out of 20 (45%) were "unchanged", 5 out of 20 (25%) were "slightly improved", and 6 out of 20 (30%) were "improved" after 1 month of initial use, as compared with before use. For the state of the white hair root of the lateral head, 1 out of 20 (5%) was "unchanged", 11 out of 20 (55%) was "slightly improved", and 8 out of 20 (40%) was "improved" after starting to use for 2 months compared to before use. For the state of the white hair root of the lateral head, 8 out of 19 (42%) were "slightly improved" and 11 out of 19 (58%) were "improved" after 3 months of initial use compared to before use.

A questionnaire using Visual Analogue Scoring (VAS) was conducted on the experimenter's degree of care about poliosis. In the VAS, the evaluation value in the case of careless white hair was set to 0, and the evaluation value in the case of careless white hair was set to 10. As a result, as shown in fig. 7, it was revealed that the essence of the examples was not intended for white hair after the use. In addition, the change rate (%) of VAS means improvement in the case of more than 105% and deterioration in the case of less than 95%. As shown in fig. 8, it is shown that the attention of the white hair was improved after starting to use the essence according to the examples.

A questionnaire using VAS was conducted on the experimenter regarding the amount of white hair. In the VAS, the evaluation value in the case of increasing the amount of white hair was set to 0, and the evaluation value in the case of decreasing the amount of white hair was set to 10. As a result, as shown in fig. 9, the amount of white hair was reduced after starting to use the essence according to the example. Further, as shown in fig. 10, it is shown that the amount of white hair was improved after starting to use the essence according to the examples.

A questionnaire using VAS was conducted on the experimenter regarding white hair color. In the VAS, the evaluation value in the case of lightening the white hair color was set to 0, and the evaluation value in the case of lightening the white hair color was set to 10. As a result, as shown in fig. 11, the white hair color became dark after the use of the essence according to the example was started. Further, as shown in fig. 12, it was revealed that the white hair color was improved after starting to use the essence according to the examples.

The experimenter conducted a questionnaire using VAS on the white hair range. In the VAS, the evaluation value in the case where the white hair range is increased is set to 0, and the evaluation value in the case where the white hair range is decreased is set to 10. As a result, as shown in fig. 13, the range of white hair was reduced after starting to use the essence according to the examples. As shown in fig. 14, the range of white hair was improved after starting the use of the essence according to the examples.

A questionnaire using VAS was conducted on the subjects regarding the level of white hair. In the VAS, the evaluation value in the case where white hair became conspicuous was set to 0, and the evaluation value in the case where white hair was no longer conspicuous was set to 10. As a result, as shown in fig. 15, it was revealed that the white hair was no longer noticeable after the use of the essence according to the examples was started. As shown in fig. 16, the improvement of the graying hair level after the start of the use of the essence according to the examples was demonstrated.

The practitioner confirms scalp itching and scalp abnormality of the examined area to the experimenter. As a result, as shown in fig. 17, the scalp state was not confirmed.

Comparative example

An essence (cosmetic liquid) according to comparative example was prepared which contained 0.001 wt% of palmitoyl tetrapeptide-20, but contained no acetyl tetrapeptide-3, red clover extract, trifluoroacetyl tripeptide-2, apple fruit culture stem cell extract, monzona growth point stem cell lysate and Liu Lanhua/leaf/stem extract, and other compositions were the same as those according to examples.

20 adults 20 years of age 20 to 69 years of age (average age 44 years) were selected as test participants. Before the experimenter started to use the essence of the comparative example, the doctor and the hair diagnostician observed white hair on the top and side of the experimenter's head. In addition, doctors and hair diagnosticians observe the hair color of the hair root of the lateral head (hairline at the top of the helix) of the experimenter using a microscope.

After that, the experimenters (1 experimenter no longer participated in the test for personal reasons) shampoo with 1 shampoo containing an antioxidant every day for 3 months, and the essence related to the comparative example was applied to the entire scalp. Meanwhile, the experimenter is prohibited from using other shampoos, hair cosmetics, white hair dyes, and the like. After starting to use the essence of the comparative example, the physician and the hair diagnostician observe the experimenters' hair on

months

1, 2 and 3.

Fig. 18 and 19 show the evolution of the improvement ratio of the white hair amount of the experimenter observed by the doctor and the hair diagnostician. The observation reference is the same as the example. For the amount of white hair at the top of the head, 6 out of 19 (31%) were "unchanged", 10 out of 19 (53%) were "slightly improved", and 3 out of 19 (16%) were "improved" after 1 month of initial use, as compared to before use. For the amount of white hair at the top of the head, 6 out of 19 (32%) were "unchanged", 12 out of 19 (63%) were "slightly improved", and 1 out of 19 (5%) were "improved" after starting to use for 2 months, as compared with before use. For the amount of white hair on the top of the head, 13 out of 19 (68%) were "slightly improved" and 6 out of 19 (32%) were "improved" after 3 months of initial use, as compared to before use.

Therefore, the use of the essence according to the comparative example results in a lower improvement in the amount of white hair at the top of the head than the use of the essence according to the example.

For the white hair amount of the lateral head, 7 out of 19 (37%) were "unchanged", 11 out of 19 (58%) were "slightly improved", and 1 out of 19 (5%) were "improved" after 1 month of starting use, as compared with before use. For the white hair amount of the lateral head, 3 out of 19 (16%) were "unchanged", 15 out of 19 (79%) were "slightly improved", and 1 out of 19 (5%) were "improved" after starting to use for 2 months, as compared with before use. For the white hair amount of the lateral head, 16 out of 19 (84%) were "slightly improved" and 3 out of 19 (16%) were "improved" after 3 months from before use.

Therefore, the improvement degree of the white hair amount of the lateral head portion is lower in the case of using the essence of the comparative example than in the case of using the essence of the example.

The evolution of the improvement ratio of the white hair color of the experimenter, which is observed by the doctor and the hair diagnostician, is schematically shown in fig. 20 and 21. The observation reference is the same as the example. For the white hair color at the top of the head, 8 out of 19 (42%) were "unchanged", 9 out of 19 (47%) were "slightly improved", and 2 out of 19 (11%) were "improved" after 1 month of initial use, as compared to before use. For the white hair color at the top of the head, 5 out of 19 (26%) were "unchanged", 12 out of 19 (63%) were "slightly improved", and 2 out of 19 (11%) were "improved" after 2 months of initial use, as compared to before use. For the white hair color at the top of the head, 12 out of 19 (63%) were "slightly improved" and 7 out of 19 (37%) were "improved" after 3 months of initial use, as compared to before use.

Therefore, the use of the essence according to the comparative example results in a lower improvement in the white hair color at the top of the head than the use of the essence according to the example.

For the white hair color of the lateral head, 8 out of 19 (42%) were "unchanged" and 11 out of 19 (58%) were "slightly improved" after 1 month of initial use compared to before use. For the white hair color of the lateral head, 4 out of 19 (21%) were "unchanged" and 15 out of 19 (79%) were "slightly improved" after 2 months of initial use compared to before use. For the white hair color of the lateral head, 1 out of 19 (5%) was "unchanged", 14 out of 19 (74%) was "slightly improved", and 4 out of 19 (21%) was "improved" after 3 months of initial use, as compared to before use.

Therefore, the improvement degree of the white hair color of the lateral head portion is lower in the case of using the essence of the comparative example than in the case of using the essence of the example.

Fig. 22 and 23 show the evolution of the improvement ratio of the white hair root of the experimenter observed by the doctor and the hair diagnostician. The observation reference is the same as the example. For the state of the white hair root at the top of the head, 8 out of 19 (42%) were "unchanged" and 11 out of 19 (58%) were "slightly improved" after 1 month of initial use compared to before use. For the state of the white hair root at the top of the head, 8 out of 19 (42%) were "unchanged" and 11 out of 19 (58%) were "slightly improved" after starting to use for 2 months compared to before use. For the state of the white hair root at the top of the head, 1 out of 19 (5%) was "unchanged", 13 out of 19 (69%) was "slightly improved", and 5 out of 19 (26%) was "improved" after 3 months of initial use, as compared with before use.

Therefore, the improvement degree of the white hair root at the top of the head is lower in the case of using the essence of the comparative example than in the case of using the essence of the example.

For the state of the white hair root of the lateral head, 8 out of 19 (42%) were "unchanged", 9 out of 19 (47%) were "slightly improved", and 2 out of 19 (11%) were "improved" after 1 month of initial use, as compared with before use. For the state of the white hair root of the lateral head, 3 out of 19 (16%) were "unchanged", 10 out of 19 (53%) were "slightly improved", and 6 out of 19 (31%) were "improved" after starting to use for 2 months, as compared with before use. For the state of the white hair root of the lateral head, 10 out of 19 (53%) were "slightly improved" and 9 out of 19 (47%) were "improved" after 3 months of initial use compared to before use.

Therefore, the improvement degree of the white hair root of the lateral head portion was lower in the case of using the essence of the comparative example than in the case of using the essence of the example.

Claims

1. Use of a Greyverse registered trademark, a Capixyl registered trademark, a Progeline registered trademark, an apple stem cell lysate, a bamboo stem cell lysate, and a deflenscap registered trademark for the manufacture of a hair care composition for preventing white or grey hair, characterized in that the composition comprises:

1.0% by weight or more of Grey verse registered trademark,

More than 2.5 weight percent of Capixyl registered trademark,

0.05 wt% or more of Progeline registered trademark,

0.009 wt% or more of apple stem cell lysate,

0.00625 wt% or more bamboo stem cell lysate

And 0.1 wt% or more of a Defenscalp registered trademark.

2. The use according to claim 1, characterized in that,

the composition can increase melanin in hair.

3. The use according to claim 1 or 2, characterized in that,

the composition is shampoo.

4. The use according to claim 1 or 2, characterized in that,

the composition is a skin caring liquid.

5. Use of palmitoyl tetrapeptide-20 or palmitoyl tetrapeptide-20 amide, acetyl tetrapeptide-3, trifluoroacetyl tripeptide-2, bamboo stem cell lysate, and willow herb extract for the manufacture of a hair care composition for preventing white or grey hair, characterized in that the composition comprises:

0.0005 wt% or more of palmitoyl tetrapeptide-20 or palmitoyl tetrapeptide-20 amide,

0.00069% by weight or more of acetyl tetrapeptide-3,

0.00001 wt% or more of trifluoroacetyl tripeptide-2,

0.00625 wt% or more bamboo stem cell lysate

0.005 wt% or more of an extract of willow herb.

6. Use of palmitoyl tetrapeptide-20 or palmitoyl tetrapeptide-20 amide, acetyl tetrapeptide-3, trifluoroacetyl tripeptide-2, bamboo stem cell lysate, and flower extract of red clover for the manufacture of a hair care composition for preventing white or grey hair, characterized in that the composition comprises:

0.00069% by weight or more of acetyl tetrapeptide-3,

0.00001 wt% or more of trifluoroacetyl tripeptide-2,

0.00625 wt% or more bamboo stem cell lysate

0.00063% by weight or more of a flower extract of Trifolium pratense.

7. The use according to claim 5, characterized in that,

the willow herb extract is contained in the Defenscalp registered trademark.

8. The use according to claim 6, characterized in that,

the flower extract of red clover is contained in the registered trademark of Capixyl.

9. The use according to claim 5 or 6, characterized in that,

palmitoyl tetrapeptide-20 amides are included in the Greyverse registered trademark.

10. The use according to claim 5 or 6, characterized in that,

acetyl tetrapeptide-3 is included within the Capixyl registered trademark.

11. The use according to claim 5 or 6, characterized in that,

trifluoroacetyl tripeptide-2 is included within the Progeline registered trademark.

12. The use according to claim 5 or 6, characterized in that,

the composition can increase melanin in hair.

13. The use according to claim 5 or 6, characterized in that,

the composition is shampoo.

14. The use according to claim 5 or 6, characterized in that,

the composition is a skin caring liquid.