TWI679210B - Method for obtaining keratin from feathers - Google Patents

Abstract

A method for obtaining keratin from feathers, which uses feather material as a raw material, uses a reaction tank containing inner and outer tanks, and an appropriate pharmaceutical ratio to sequentially soften and dissolve the feathers, so that the reaction can be completed quickly. , Dissolving, centrifuging, filtering and other processes, high-efficiency production of keratin solution, helps the reuse of feather waste, and improves the problem of low added value.

Description

Method for obtaining keratin from feathers

The invention relates to a method for extracting keratin from feathers, in particular to a method for extracting keratin suitable for use in the field of chemical engineering of the people's livelihood. The energy-saving, environmental protection, and high-efficiency mode enables the use of existing feather fine-washing equipment at a low cost. Production will help relevant operators to handle and utilize feather waste.

The material of down products is mainly taken from the feathers of waterfowl poultry (such as ducks and geese), and the feathers of waterfowl poultry are divided into Down and Feather with feather stems / feather shafts. Feathers are not as effective as feathers in keeping warm and have a large proportion (about 90% of the total body of a duck or goose). Most of them are not suitable as fillings for down products, and they are mostly waste or are subjected to high temperature. After high-pressure hydrolysis, it is made into livestock feed, but because the feathers of ducks and geese are full of keratin and disulfide bonds, the animal's digestive system will not be digested without high hydrolysis, and the added value is low.

However, feathers grow from the skin of poultry. During the growth process, the skin's keratin is covered with feathers and grows. Therefore, feathers mostly contain about 80% of keratin; currently known techniques for extracting keratin from feathers and producing energy are mostly Disulfide bond (disulfide bond) is thermally cracked by high temperature (about 120 ℃ ~ 200 ℃) and pressurized by hydrolysis method, but the utilization rate of amino acid is not ideal. Carbonization reaction is easy to occur during hydrolysis. The protein product is brownish black.

A method for extracting keratin from feathers was proposed in U.S. Patent No. 8575513. Although the content disclosed in it discloses that the yield can be improved, the potassium cyanide (KCN) used is highly toxic, which increases the operation or use of Risks, and harm to the environment, and its use of ammonium sulfate (NH ₄ ) ₂ SO ₄ precipitation method will make the wastewater produced contain a large amount of NH ₄ ⁺ and SO ₄ ^- ions, making wastewater treatment operations or environmental protection more costly work.

In addition, Japanese Patent No. 2009298737 discloses a method for producing keratin from poultry feathers. The disclosed processes and the use of medicaments are cumbersome, and the precipitate must be continuously adjusted by adjusting the pH value. The overall proportion of water bath used is high, 1g Feathers with 50ml of mercaptoacetic acid solution (alias TGA) require a lot of water resources and formulated chemicals; finally, the pH value is adjusted to 3.5 with acetic acid, and the resulting keratin product is acidic, which is not suitable for use as a consumer product (such as makeup, hair , Skin care products).

In addition, the above two methods collect the sediment by centrifugation, and the keratin products need to be separated at a high speed (10000 rpm). Such high-speed centrifuge equipment is laboratory-grade equipment, which not only has a small capacity and a limited output (only about Load 1L of solution), and the high equipment cost is not conducive to mass production. Furthermore, the previously known methods for extracting keratin from feathers, from the reaction system to the filtration system to the centrifugal system, are all operated by independent equipment, so the equipment used High cost, easy to form wear and tear in the program, and cleaning, maintenance and other operations are not easy.

Keratin is the main purchased material for human skin, hair and nails, so keratin has high value in cosmetics, biomedicine and other industries of the people's livelihood and chemical industry. Therefore, how can a large amount of feather waste be added in an efficient manner? Value, is the subject of continuous improvement and improvement of down related industry.

Methods for extracting keratin include mechanical methods such as heat and pressure, reduction methods that use a reducing agent to destroy the bonding structure, and ionic liquid methods that are relatively safe and harmless to the biological environment. Depending on the application, suitable extraction methods can be adopted. For example, feed can be produced by simple mechanical methods with low purity, and ionic liquid methods can be used in biomedical applications. The invention is mainly used in the field of chemical industry for people's livelihood, and combines oxidation and reduction to achieve the effects of softening feather keratin and accelerating dissolution, and is a method for dissolving feathers with high efficiency and large yield, thereby obtaining keratin rich in feathers; Therefore, the present invention can be applied to existing equipment (such as a large-capacity down washing machine), and can quickly complete the processes of reaction, dissolution, centrifugation, and filtration in a single container. The keratin solution can be efficiently produced and can be effectively improved. A large amount of feather waste is difficult to use and has low added value.

In addition, the present invention utilizes a non-toxic drug commonly used by Minsheng Chemicals, and uses a sulfide-containing mode to open the disulfide bond of feathers without causing harm to human health. The keratin produced can be safely used in Minsheng products. In addition, the present invention applies a method of extracting different pH and molecular weight by oxidation and reduction, which can greatly reduce the reaction time and obtain high-yield keratin (the total operation time does not exceed 2 hours) to obtain keratin with a single interval of molecular weight. The process used Simple and low energy consumption and low medicine cost.

The method for obtaining keratin from feathers according to the present invention mainly includes a reaction tank, which includes an inner tank and an outer tank with a receiving space. The inner tank is arranged in the outer tank, is provided with a plurality of fine holes and can be driven in The outer tank rotates; in general, the reaction tank can be made by the existing fine cleaning equipment of the down / feather fine cleaning factory, with a simple modification of the medicine supply system, etc., and can be applied to the mass production process of the present invention.

During the operation of the present invention, the main process includes: 1. placing a selected feather material into an inner groove; 2. Inject water and softening agent into the reaction tank according to the ratio, and mix to form an aqueous solution containing the softening agent to soak the feather material; 3. soften the feather material with a certain time to soften the feather material; 4. add the dissolved agent to the aqueous solution, The temperature of the aqueous solution in the reaction tank was raised to 85 ° C; 5. The dissolution reaction was performed within a certain time to dissolve the feather material in the aqueous solution; 6. The inner tank was driven to rotate and discharged from the outer tank discharge port, and centrifuged to obtain Keratin in water.

The feather material obtained by the present invention is mainly selected from feathers that are not suitable for use as down products. These feathers are poultry feathers with peduncles or shafts that have been finely washed. Preferably, these feathers may also be cut or The processed feather scraps are pulverized, and feather scraps of an appropriate length (for example, 1 to 3 cm) are selected as the feather material for making a keratin solution according to the present invention.

The weight ratio of feather material to aqueous solution used in each operation can be adjusted between 1: 5 to 1:12. With sufficient reaction tank capacity, even more than 200 kg of feather material can be put into each operation to facilitate Mass production and disposal of large quantities of feather waste.

During the softening reaction, the inner tank is driven to operate properly (about one rotation in about 2 seconds), constantly stirring the feather material and the aqueous solution for about 30 minutes, so that the softening agent is evenly distributed and can fully react with the feather material; The softening agent can be H ₂ O ₂ or NaOH, and the aqueous solution prepared by the two can accelerate the oxidation reaction with feathers at the appropriate temperature and concentration, so as to initially destroy the outer structure of the feathers and soften the keratin. Among them, the temperature of the aqueous solution is preferably 45 to 60 ° C, and the concentration is preferably 15 to 50% H ₂ O ₂ solution or 0.5 to 1 N NaOH solution. Among them, H ₂ O ₂ has a bleaching effect and helps the final A milky white keratin solution is formed, and the reaction time of 30 minutes can reduce the concentration of H ₂ O ₂ in the aqueous solution to less than 2 mg / L without disturbing the subsequent dissolution reaction (reduction reaction). Similarly, the NaOH component does not Can interfere with subsequent dissolution reactions.

During the softening reaction, it can also drive the inner tank to operate properly (about one rotation in about 2 seconds), constantly agitating the softened feather material and the aqueous solution added with the dissolving agent, and maintaining it for 30 to 60 minutes to dissolve the agent. Spread evenly and fully react with softened feather material. The main components of the dissolving agent include: (1) SDS (Sodium dodecyl sulfate), (2) Urea (Carbamide), (3) βME (β-mercaptoethanol, β-mercaptoethanol, 2- Mercaptoethanol), DTT (D, L-Dithiothreitol, Dithiothreitol) or Thioura Dioxide (Thiourea Dioxide); these three ingredients are added to the aqueous solution in an appropriate ratio, which can quickly promote softened feathers The material (the keratin structure on the surface is destroyed) is dissolved in the aqueous solution to form an aqueous solution rich in keratin; preferably, the concentration of the first component SDS in the aqueous solution can be adjusted between 0.003 ~ 0.02M, and the second component Urea is at The concentration in the aqueous solution can be adjusted between 0.06 ~ 0.6M, and the concentration of the third component in the aqueous solution can be adjusted between 0.08 ~ 0.1M using βME, and can be adjusted between 0.01 ~ 0.03M using DTT. Thiourea dioxide can be adjusted between 0.02 ~ 0.05M.

During the centrifugal separation of the aqueous solution in the inner tank, the inner tank is driven to rotate at 800 to 1000 rpm for about 5 minutes, and the 20 to 50 mesh pores (the pore size is equivalent to between 0.3 to 0.85 mm) opened in the inner tank. To separate undissolved feather material from keratin-containing water solutions.

Due to the hard texture of the peduncle / pinnacle, it usually occupies a large volume. If the bath is to cover all the raw materials for reaction, it will consume too much water and chemicals. In the present invention, the softening step can be used to destroy the peduncle / pindle. The outer stratum corneum allows the dissolving agent to infiltrate smoothly and accelerates the efficiency of the dissolving reaction, and the volume of the feather material is reduced after the softening of the material, which is more likely to be wet by the agent, reducing the amount of water and Dosing of medicament dose.

The method for obtaining keratin from feathers according to the present invention described above has the following advantages:

(1) Mass production, applicable to the existing equipment of the feather cleaning plant. According to the currently known equipment, more than 200 kilograms of feather material can be put in each batch, which can improve the problem of large feather waste disposal by the down industry and greatly increase Its added value.

(2) Efficiency. The process of each batch of the present invention is far lower than the process of more than 6 hours (US-8575313) of known feather dissolution, and the currently measured yield can reach 95 kg / h.

(3) Energy saving. In addition to the formulation of the medicine, the temperature of the feather dissolution rate is also a factor affecting the dissolution rate. The temperature of the overall process of the present invention is lower than 85 ° C, and the operation time is short, the heating time does not exceed 2 hours, and the energy consumption is much lower than Currently known processes.

(4) Environmental protection. The medicaments used in the present invention are all currently non-toxic toxic medicaments commonly used by Minsheng Chemicals. Keratin products can be safely applied to Minsheng products.

(5) Low cost, combining the aforementioned features, and the equipment used can meet the needs of the overall process. Its reaction tank, centrifugal, and filter functions can be combined into one. There is no need to use most equipment to respond to the process. The mechanism is simple and easy to clean and maintain. It is equipped with automation. Equipment can reduce personnel costs.

10‧‧‧ reaction tank

11‧‧‧ Outer trough

12‧‧‧Inner slot

13‧‧‧Feeding port

14‧‧‧Discharge port

15‧‧‧Drive System

16‧‧‧ medicine supply system

17‧‧‧Circulation system

S1‧‧‧Softening reaction

S2‧‧‧ Dissolution reaction

S3‧‧‧ Centrifugal Filtration

FIG. 1 is a schematic flowchart of the method of the present invention.

Fig. 2 is a schematic diagram of a reaction tank of the present invention.

Keratins are alpha keratin (α-keratin) and beta keratin (β-keratin), alpha keratin The protein is mainly found in mammals, and β-keratin is mainly distributed in birds and reptiles. The feather stems / feather shafts of bird feathers have a honeycomb-like tissue in the center and have a dense, strong outer layer. Tough cuticle, under normal circumstances, it is difficult to dissolve the dissolving agent, and it often takes a lot of time and medicine to dissolve the feathers; as shown in Figure 1, the main concept of the method of the present invention is to soften the reaction (pre-dissolving treatment) S1 first. Destroy the outer structure and soften the feathers. The dissolving agent can quickly penetrate the center during the subsequent dissolution reaction S2 to achieve the effect of rapid dissolution. It can be used for a large amount of feather waste with low added value (with stems / feathers, not suitable for down products). , And dissolve it to obtain β-keratin.

Please refer to FIG. 1 and FIG. 2 together. This embodiment mainly uses a reaction tank 10 to dissolve feathers, and simultaneously completes the processes (softening reaction S1, dissolution reaction S2), and centrifugal filtration S3 to quickly obtain a keratin solution; The reaction tank 10 has an outer tank 11 and an inner tank 12. The inner tank 12 is disposed in the outer tank 11 and is provided with a plurality of fine holes (about 20 to 50 mesh), and the inner tank 12 can be driven by one. The system 15 is driven to rotate relative to the outer tank 11.

In this method, the raw materials (feather materials) are selected first. In this embodiment, the feather pieces that have been cut and washed are finely washed, and then the selected feather materials are placed in the inner tank from the feed port 13. In 12, the appropriate amount of water is injected according to the weight of the feather material. The drug supply system 16 is used to inject a softening agent with a suitable concentration, which is mixed to form an aqueous solution containing the softening agent to soak the feather material. When the softening reaction S1 is performed, it is transmitted through the drive system. 15 Drive the inner tank 12 to rotate continuously and in small amounts (about two seconds per revolution) to form a state of constant agitation. At the same time, with the circulation system 17, the aqueous solution flowing out of the inner tank 12 series of holes is repeatedly injected into the inner tank 12 to form a cycle to accelerate the medicine. It diffuses and can react with feathers. After a period of time, the softening reaction S1 is completed to form a softened feather material.

After the softening reaction S1, the medicine supply system 16 is used to dissolve the prepared dissolving medicine. Agent is added to the aqueous solution, and the aqueous solution in the reaction tank 10 is heated to 85 ° C. to perform the dissolution reaction S2; when the dissolution reaction S2 is performed, the inner tank 12 is also driven by the driving system 15 for a small amount of rotation (about two seconds per revolution) ) To form a state of constant agitation. At the same time, the aqueous solution flowing out of the pores is repeatedly injected with the circulation system 17 to accelerate the diffusion of the agent and the feather reaction. After a period of time, the dissolution reaction S2 is completed and most of the feather material (more than 90%) is dissolved In aqueous solution.

After the dissolution reaction S2, the process of centrifugal filtration S3 is performed. At this time, the inner tank 12 is driven to rotate rapidly (about 800 rpm) through the drive system 15 and the discharge port 14 of the outer tank 11 is opened for discharge for about five minutes. Then, the aqueous solution discharged from the discharge port 14 can be collected to obtain a keratin solution after the feather material is dissolved, and the feather material that is not completely dissolved is left in the inner tank 12.

In this method, the softening agent used in the pre-dissolving treatment (softening reaction S1) is hydrogen peroxide or sodium hydroxide (NaOH), which is an oxidant, which can achieve the effect of destroying the outer layer of the plume / feather shaft and softening the feathers. The effect of removing grease and impurities; among them, the chemical nature of hydrogen peroxide is unstable, so the concentration, temperature, and time are the main factors that affect the chemical reaction. After the softening reaction S1, the hydrogen peroxide concentration is reduced to about 2m / L without affecting subsequent dissolution. The reaction causes interference. Through experiments, hydrogen peroxide has a better concentration of 15-50% aqueous hydrogen peroxide solution. The main factor affecting the chemical reaction of NaOH is concentration. If it is too low, it will not respond well. If it is too high, it will overreact and it is easy to make feathers. Coking. After experiments, NaOH acts on NaOH solutions with a better concentration of 0.5-1N.

The β-keratin in the feather is folded into the main chain. The method is to extract the keratin by opening the side chain bond without breaking the main chain. The dissolution process is to open the disulfide bond of the side chain of the feather molecule by reduction method, and use a reducing agent ( (Such as β-mercaptoethanol, thiourea dioxide, and dithiothreitol) reduce the disulfide bond in the protein molecule to a thiol group. Because the thiol group is unstable, it is easy to form new disulfide bonds in the air. Combined with a surfactant (such as sodium lauryl sulfate) to prevent the aggregation of different keratin chain molecules and avoid secondary bonding; in addition, the use of urea can destroy non-polar functional groups, increase the hydrophilicity of keratin, and improve the dissolution rate. Through experiments, the preferred concentration of reducing agent on feathers is 0.08 ~ 0.1M β-mercaptoethanol (βME), 0.01 ~ 0.03M dithiothreitol (DTT) or 0.02 ~ 0.05M thiourea dioxide as The preferred surfactant is 0.003 ~ 0.02M sodium lauryl sulfate (SDS); the urea (Urea) is preferably 0.06 ~ 0.6M.

During the reaction process, turning the inner groove 12 can accelerate the feathers to be wetted by the medicine and react. The automatic medicine supply system 16 is used to accurately measure the required medicine dose. The liquid circulation system 17 helps to accelerate the medicine diffusion and soften the reaction. Or continuously agitate during the dissolution reaction, so that the required agent (softening agent, dissolving agent) can be evenly distributed at any time, and the most suitable concentration of the stroke reacts with the feather. After the disulfide bond of the feather is opened, it can be dissolved in water to form a keratin solution; After that, the inner tank 12 is rotated at a high speed, and the keratin solution and the undissolved matter are filtered by using the centrifugal force and the inner pores of the inner tank. The keratin solution can be obtained from the discharge port 14 of the outer tank 11; the final keratin solution can be reused for purification. (Such as salting out, dialysis, chromatography, etc.) The chemicals used are separated to obtain a high-purity keratin solution.

The following are several preferred embodiments of the method according to the above:

Embodiment one:

1. Fill 1 kg of feather material (with peduncle / feather shaft, washed and crushed) into the inner tank; 2. Inject water and hydrogen peroxide (softening agent) and mix to form an aqueous solution with a hydrogen peroxide concentration of about 15-20%. It is about 10 times the weight of feathers (such as 6 liters of clear water and 4 liters of 50% hydrogen peroxide), and the temperature reaches 45 ° C to 60 ° C; 3. Drive the inner tank to continuously agitate for two seconds and maintain a softening reaction for 30 minutes ; 4. Add the configured dissolving agent (containing SDS, Urea, βME), and mix into the solution An aqueous solution containing 0.02M SDS, 0.6M Urea, and 0.1M βME (such as about 150g of SDS, 1kg of Urea, and 150g of βME) is heated to 85 ° C; 5. Drive the inner tank to continuously agitate for two seconds, Maintain the dissolution reaction for 30-60 minutes; 6. Drive the inner tank at a speed of about 800 rpm and run for about five minutes to remove the undissolved matter and the keratin-containing aqueous solution after the feathers are dissolved, and collect the angle from the outer tank discharge port Protein solution.

In the first embodiment, the dissolution rate of feathers is 93%, and the average operating time is about 1.5 hours. The obtained product is a keratin solution with a molecular weight of about 4-6 kDa, a pH of 4.7-5.0, and can be applied to washing and skin care products. , Larger molecular weight, the interaction between natural keratin and skin surface keratin to achieve moisturizing effect.

Embodiment two:

1. Fill 1 kg of feather material (with peduncle / feather shaft, washed and pulverized) into the inner tank; 2. Inject water and hydrogen peroxide (softening agent) and mix to form an aqueous solution with a hydrogen peroxide concentration of about 35-50%. It is about 10 times the weight of feathers, and the temperature reaches 60 ° C. 3. Drive the inner tank to continuously agitate for two seconds and maintain for 30 minutes for the softening reaction. 4. Add the configured dissolving agent (containing SDS, Urea, βME ), Mixed into an aqueous solution to form an aqueous solution containing 0.02M SDS, 0.6M Urea and 0.08M βME, and heated to 85 ° C; 5. Drive the inner tank to continuously agitate for two seconds and maintain a dissolution reaction for 30 minutes; 6. Drive the internal The tank is drained at a rotation speed of about 800 rpm for about five minutes, the insoluble matter and the keratin-containing aqueous solution after the feathers are dissolved are separated, and the keratin solution is collected from the outer tank discharge port.

In the second embodiment, the feather dissolution rate is 95%, and the average operating time is about 1 hour. The obtained product is a white keratin solution with a molecular weight of about 1 to 3 kDa, a pH of 4.2 to 4.6, Can be applied to washing, hair care, skin care products, small molecular weight is easier to enter the tissue, such as penetrating into the cortex, giving elastic effect.

Embodiment three:

1. Fill 1kg of feather material (with peduncle / shaft, washed and crushed) into the inner tank; 2. Inject water and NaOH (softening agent) and mix to form an aqueous solution with a NaOH concentration of about 0.5N. The aqueous solution is about The feathers are 10 times heavier and the temperature reaches 60 ° C; 3. Drive the inner tank to continuously agitate for two seconds and maintain the softening reaction for 30 minutes; 4. Add the configured dissolving agent (containing SDS, Urea, βME), Mix into the aqueous solution to form an aqueous solution containing 0.005M SDS, 0.25M Urea and 0.08M βME, and raise the temperature to 85 ° C; 5. Drive the inner tank to stir continuously for two seconds and maintain a dissolution reaction for 60 minutes; 6. Drive the inner tank to The rotation speed is about 800 rpm, and the solution is drained for about five minutes. The insoluble matter and the aqueous solution containing keratin after the feathers are dissolved are separated, and the keratin solution is collected from the outer tank discharge port.

In the third embodiment, the feather dissolution rate is 90%, and the average operating time is about 1.5 hours. The obtained product is a light yellow keratin solution with a molecular weight of about 7-10 kDa, a pH of 7-7.7, and can be applied to hair care products. , Effectively repair the damaged area, adhere to the surface of the hair, prevent the loss of protein from the inside of the hair, and achieve a durable effect.

Embodiment 4:

1. Fill 1 kg of feather material (with peduncle / shaft, washed and crushed) into the inner tank; 2. Inject water and hydrogen peroxide (softening agent) and mix to form an aqueous solution with a hydrogen peroxide concentration of about 25%. The aqueous solution is about The feathers are 10 times heavier and the temperature reaches 60 ° C; 3. Drive the inner tank to continuously agitate in a circle of two seconds and maintain the softening reaction for 30 minutes; 4. Add the configured dissolving agent (containing SDS, Urea, DTT), mix into the aqueous solution to form an aqueous solution containing 0.003M SDS, 0.06M Urea, and 0.02M DTT, and raise the temperature to 85 ° C; 5. Drive the inner tank for two seconds Agitate continuously for 60 minutes for the dissolution reaction; 6. Drive the inner tank to run at a speed of about 800 rpm for about five minutes to remove the undissolved matter and the keratin-containing aqueous solution after the feathers are dissolved, and discharge from the outer tank. The keratin solution was collected in the mouth.

In the fourth embodiment, the dissolution rate of feathers is 95%, and the average operating time is about 1.5 hours. The obtained product is a keratin solution with a molecular weight of about 4-5 kDa, a pH of 4.7-5.0, and a milky white color. The DTT used is safer. βME is much higher. It is mixed with functional polymers and cross-linked to produce a composite film, which can be used in medical materials such as wound dressings and artificial skin in the future.

Embodiment 5:

1. Fill 1 kg of feather material (with peduncle / feather shaft, washed and pulverized) into the inner tank; 2. Inject water and NaOH (softening agent) and mix to form an aqueous solution with a NaOH concentration of about 1N. The aqueous solution is about feathers 10 times the weight and make the temperature reach 60 ℃; 3. Drive the inner tank to continuously agitate for two seconds and maintain the softening reaction for 30 minutes; 4. Add the configured dissolving agent (containing SDS, Urea, thiourea dioxide), Mix into the aqueous solution to form an aqueous solution containing 0.005M SDS, 0.25M Urea and 0.05M thiourea dioxide, and raise the temperature to 85 ° C; 5. Drive the inner tank to stir continuously for two seconds and maintain a dissolution reaction for 30 minutes; 6. Drive the inner tank Run at a speed of about 800 rpm for about five minutes to remove the keratin solution from the undissolved matter and the keratin-containing aqueous solution after the feathers are dissolved, and collect the keratin solution from the outer tank discharge port.

In the fifth embodiment, the dissolution rate of feathers is 93%, and the average operating time is about 1 hour. The product obtained is a keratin solution with a molecular weight of about 5-7 kDa, pH 7-8, and a milky white color. The molecular weight is slightly Larger, more hydrophobic, you can add nail polish, mascara, lip balm and other products to adjust the moisturization of the stratum corneum and effectively make the hair shape more stable.

As in the above examples, the keratin solution after the oxidation (softening, pre-dissolution) and reduction (dissolution) reactions of this method (because the keratin solution has a large molecular weight and long chain shape, and the solution concentration is high, the viscosity is thick, and it is not easy to filter ), The pores in this preferred size range have been measured (20 ~ 50 mesh, the number of holes in each square inch, the pore diameter is equivalent to about 0.3 ~ 0.85mm), and the undissolved matter can be restricted well during centrifugal filtration Particles, and the method has a dissolution rate of more than 90%, and the problem that the pores are blocked and cannot be filtered due to the large number of undissolved particles will not occur.

The keratin products obtained according to the above embodiments of the method have good water solubility, and have hydrophilic and hydrophobic amino acids, which can easily dissolve keratin into hair, skin and nails, and is suitable for use in makeup and hair And skin care products; and the keratin solution obtained has a molecular weight of 1-10kDa, small molecular structure absorption speed, high solubility, suitable for human body absorption; In addition, it can also be combined with functional polymers to make solid or liquid composite materials, in the future Can be applied to medical care and green adsorption materials.

The above-mentioned implementation content is only a description of the preferred embodiments of the present invention. Any extension, modification, simple change, or equivalent replacement made according to the technical means of the present invention should also fall within the scope of the patent application of the present invention.

Claims (9)

A method for obtaining keratin from feathers, comprising: (a) providing a reaction tank including an inner tank and an outer tank, wherein the inner tank is provided with a plurality of fine holes and can be driven to rotate in the outer tank; (b) Put the feather material into the inner tank, and infuse the feather material with an aqueous solution containing a softening agent. After the reaction, the outer keratin is destroyed to soften the feather material, wherein the softening agent may be H ₂ O ₂ or NaOH; (c) the The dissolving agent is added to the aqueous solution and heated to 85 ° C. After the reaction, the feather material is dissolved in the aqueous solution. The dissolving agent includes a reducing agent, a surfactant, and Urea (urea). The reducing agent may be βME ( β -mercapto Ethanol), DTT (dithiothreitol) or thiourea dioxide, the surfactant can be SDS (sodium dodecyl sulfate); (d) the inner tank is driven to spin and centrifuge to separate the aqueous solution, and from the outer tank The discharge port is discharged to obtain an aqueous solution containing keratin. The method according to item 1 of the patent application scope, wherein the feather material is selected from a bird feather having a stalk or a feather shaft. The method according to item 2 of the scope of the patent application, wherein the weight ratio of the feather material to the aqueous solution is 1: 5 to 1:12. The method according to item 1 of the scope of patent application, wherein when step (b) is performed, the inner tank is driven to agitate the feather material and the aqueous solution for 30 minutes to complete the reaction, and the temperature of the aqueous solution is between 45 and 60 ° C . The method according to item 1 of the scope of patent application, wherein when step (c) is performed, the inner tank is driven to agitate the feather material and the aqueous solution, and is maintained for 30 to 60 minutes to complete the reaction. The method according to item 1 of the scope of patent application, wherein in step (d), the inner groove is rotated at 800 to 1000 rpm for about 5 minutes, and the undissolved feather material and the Keratin aqueous solution is isolated. The method according to item 1 of the scope of the patent application, wherein the aqueous solution containing the softening agent is a H ₂ O ₂ solution having a concentration of 15-50% or a NaOH solution having a concentration of 0.5-1N. The method according to item 7 of the scope of the patent application, wherein an aqueous solution containing a dissolved pharmaceutical agent is added, and the Urea (urea) concentration thereof is 0.06 to 0.6M, and the surfactant is contained in an SDS (concentration 0.003 to 0.02M) Sodium lauryl sulfate), and the reducing agents contained are βME ( β -mercaptoethanol) at a concentration of 0.08 to 0.1M, DTT (dithiothreitol) at a concentration of 0.01 to 0.03M, or 0.02 to 0.05M Thiourea dioxide. A method for obtaining keratin from feathers, comprising: (a) immersing feather material in an aqueous solution containing a softening agent at a temperature of 45-60 ° C, and reacting for 30 minutes to destroy the outer keratin and soften the feather material, wherein, the The softening agent may be H ₂ O ₂ or NaOH; (b) adding a dissolving agent to the aqueous solution, heating to 85 ° C., and reacting for 30 to 60 minutes to dissolve the feather material in the aqueous solution, wherein the dissolving agent contains a reducing agent, Surfactant and Urea (urea). The reducing agent may be βME ( β -mercaptoethanol), DTT (dithiothreitol) or thiourea dioxide. The surfactant may be SDS (sodium lauryl sulfate); (c) Centrifugally separate the aqueous solution through a 20-50 mesh pore to obtain an aqueous solution containing keratin.