TWI678966B - Cell dispensing and storage device and method - Google Patents

Abstract

The creative system is a cell dispensing and storage device and a method thereof. The method includes: moving a freezing box containing a test tube and a temporary storage bottle containing a cell specimen to a dispensing area, and transferring the temporary storage bottle and a injection The liquid components are connected, a dispensing area fixture then moves the test tube on the freezer box to a test tube holder on a rotating platform, and then rotates through the rotating platform. The test tube passes through a clamp head and the cover on the test tube is removed. Then, a liquid injection nozzle is used to inject the cell specimen in the temporary storage bottle into the test tube. Finally, the test head is again passed through the gripper and the lid is closed. The test tube is clamped back to the freezing box by the clamp in the dispensing area and passed through. A transfer zone fixture and a freezing zone fixture move the test tubes on the freezer box to a freezer for storage, so that this creation can achieve automatic liquid injection and storage of cell specimens, and avoid human-induced pollution.

Description

Cell dispensing and storage device and method

The present invention relates to a cell preservation device and its process, especially an automated cell injection fluid preservation device.

During the collection, processing, and storage of cell specimens, if bacteria or other dirt are in contact with the cell specimen to be stored, it is easy to cause the cell (sample) to lose its desired or pending characteristics, so Processes such as cell storage or experimental testing are usually performed in a sterile room or in a safe environment.

In the prior art, for the storage of cells, the user first puts the cell liquid stored in the temporary storage bottle into a plurality of test tubes (freezing tubes) in batches, and then covers the test tube containing the cell liquid and puts it in a low temperature. In a liquid nitrogen storage tank.

However, because the cell fluid storage method of the prior art is performed by humans, during the process of cell storage, for example, the process of removing cells from a temporary storage bottle and injecting them into a test tube is likely to be accidentally caused by contact with the outside Contamination with bacteria or other dirt causes the cells in the test tube to lose their preservation value; therefore, the prior art cell specimen storage method has its defects.

In view of the shortcomings and deficiencies of the prior art, the present invention provides a cell dispensing and storage device and a method thereof. By automating the overall process of cell storage, it can save manpower and reduce the infection of cells during storage and make them unusable. risk.

In order to achieve the above-mentioned creation purpose, the technical method adopted in this creation is a cell dispensing and storage device, which includes a feeding area, which has a feeding opening, which is connected to the outside; a transmission area, It is selectively communicated with the feeding area. The transmission area is provided with a transmission area fixture. The transmission area fixture is movably disposed in the transmission area. At least one access opening is further formed on a wall surface of the transmission area. ; A sub-packing area, which is selectively connected with the transmission area, and a sub-packing station is provided in the sub-packing area; a sub-packing base is movably disposed on the sub-packing station, and the sub-packing The base can be moved to the transmission area; a rotating column is rotatably arranged on the dispensing platform, and the rotating column is provided with at least one gripping head which can move up and down relative to the rotating column; a rotation A platform that is rotatably disposed on the dispensing table with respect to the rotating column; a plurality of test tube placing members are arranged on the rotating platform in a circle; the rotation of the rotating platform enables the test tube placing pieces to move around the rotating column; , The at least one gripping head Move to the top of each test tube placement piece; a dispensing area fixture, which is set on the dispensing table and can be moved above the dispensing base and above one of the test tube placement pieces; a liquid injection assembly, It is disposed on the dispensing platform, and the liquid injection assembly includes a nozzle holder, a liquid injection nozzle and a liquid injection line. The nozzle holder is disposed on the dispensing platform, and the liquid injection nozzle is installed on the nozzle holder. Is located above one of the test tube placement pieces, and one end of the liquid injection line is connected to the liquid injection nozzle; A freezing area selectively communicated with the transfer area, and a freezing cabinet and a freezing area fixture are provided in the freezing area; the freezing area fixture is movably disposed in the freezing area and can be moved to the freezing area Inside the cabinet.

In order to achieve the above-mentioned creation purpose, the technical method used in this creation is a cell dispensing and storage method, which includes the following steps: preparation steps: preparing a temporary storage bottle, a freezing box, a freezing box lid, a transfer area, A transfer area fixture, a dispensing area and a freezing area. Cell contents are placed in the temporary storage bottle. A test tube is arranged in the freezing box. The freezing box cover is arranged on the freezing box. The transferring area fixture is provided. In the transfer area, a sub-packing base, a sub-assembly fixture, a rotating column, a rotating platform, a test tube placement piece, and a liquid injection assembly are provided in the sub-packing area; a clamping and pick-up is provided on the rotating column. The liquid injection assembly includes a nozzle holder, a liquid injection nozzle and a liquid injection pipeline; a freezing cabinet and a freezing zone fixture are set in the freezing zone; and the placing steps are: the dispensing base is moved from the dispensing zone to In the transfer area, the freezing box is placed on the dispensing base, the transferring area clamp lifts the freezing box cover from the freezing box, and the dispensing base moves the freezing box from the transferring area to the dispensing base. In the loading area, the loading area fixture moves the test tube in the freezer to In the test tube placement member on the rotating platform, the nozzle holder is disposed in the dispensing area, the liquid injection nozzle is disposed on the nozzle holder, the temporary storage bottle and the liquid injection line are placed in the liquid injection assembly, and The opposite ends of the liquid injection line are respectively connected to the liquid injection nozzle and the temporary storage bottle; the tube opening step: the rotating platform moves the test tube placing piece to the rotation head below the gripping head of the rotating column, and the clip Take the head down and remove a cover of the test tube from the test tube, and then raise it; liquid injection step: the rotating platform moves the test tube placement part to the liquid injection component, the liquid injection nozzle places the temporary storage bottle The inner cell specimen is drawn out and injected into the test tube; the tube closing step: the rotating column and the gripping head rotate together, and the rotating platform moves the test tube below the gripping head, and the gripping head moves down and Install the cover on the test tube; Transfer step: The rotating platform moves the test tube placement piece to a position adjacent to the fixture of the dispensing area, and the dispensing area fixture clamps the test tube from the test piece placement piece into the freezing box; storage step: the dispensing The base moves the freezer box to the transfer area, the transfer area clamp covers the freezer cover above the freezer box, and then removes the freezer from the transfer area from the dispensing base and moves to In the freezer zone, the freezer clamp moves the test tube into the freezer.

The advantage of this creation is that by placing the frozen box containing the test tube into the feeding area from the feeding opening and moving it to the transfer area, at the same time moving the dispensing base from the dispensing area to the transfer area, and the transfer in the transfer area After the freezer lid on the freezer is removed from the freezer, the freezer and the test tube are placed on the dispensing base. Finally, the freezer with the test tube is moved to the dispensing area. Then, the test tube can be taken out of the freezer holder and placed on the test tube holder through the clamping of the clamp in the dispensing area, and the test tube cover of each test tube is opened by the clamping head of the rotating column; When the test tube placement piece rotates, the test tubes will pass through the infusion assembly one by one. At this time, the injection nozzle will inject the cell specimen in the temporary storage bottle into each test tube, and then the test tube placement piece is rotated to separate from the injection injection head, and The rotating column will further cover the originally opened test tube and take it through the clamp of the dispensing area, return the test tube containing the cell specimen to the freezing box, and once again pass the freezing base from the dispensing base The loading area is moved back to the transfer area. After closing the freezer lid, the transfer area clamps Move the freezing box from the transfer area to the trolley in the freezing area, and finally move the freezing box to the freezing area for storage, completing the entire process of dispensing and storing cell specimens; this creation is completely in the process of dispensing cell specimens to each test tube Automation can achieve better bacteria isolation effect in the packaging area, so it can not only avoid the possibility of contaminating the specimen produced by manual operation, but also reduce the human resources required during packaging.

Further, the aforementioned cell dispensing and storage device further includes a loading area trolley, which is disposed in the loading area, and the loading area trolley can be moved to the transfer area; a freezing area trolley, It is set in the freezing zone, and the freezing zone trolley can be moved to the transfer zone.

Further, the aforementioned cell dispensing and storage device further includes a gate assembly, and the gate assembly includes an entrance gate, which is disposed in the feeding area and the transmission area. And the entrance gate selectively closes the communication relationship between the feeding area and the transmission area; an exit gate is provided between the transmission area and the freezing area, and the exit gate selectively closes the exit gate The connection relationship between the transmission area and the freezing area; a feeding gate, which is disposed between the transmission area and the dispensing area and is adjacent to the feeding area, the feeding gate selectively closes the transmission area And the connection relationship between the repacking area; a freezing box gate, which is arranged between the transmission area and the repacking area and is adjacent to the freezing area, the freezing box gate selectively closes the transmission Area and the connection relationship of this sub-area.

Further, in the aforementioned cell dispensing and storage device, each of the at least one gripping head can rotate relative to the rotating column.

Further, in the aforementioned cell dispensing and storage device, a freezing box scanning device is further disposed in the transmission area, which is disposed adjacent to the at least one picking opening.

Further, in the aforementioned cell dispensing and storage method, from the placing step to the tube opening step, the rotating platform rotates the test tube placing member 90 degrees with respect to the rotating column in a rotating direction; from the tube opening step At the time of the liquid injection step, the rotating platform rotates the test tube placing member relative to the rotating column by 90 degrees in the rotation direction; from the liquid injection step to the tube closing step, the rotating platform rotates the test tube placing member relative to the rotating column. Rotate 90 degrees in the direction of rotation; from the step of closing the tube to the step of transferring, the rotating platform rotates the test tube placement piece 90 degrees in the direction of rotation relative to the rotating column.

Further, in the aforementioned cell dispensing and storage method, in the preparation step, an inlet gate of the transfer area is opened, and the freezing box, the liquid injection line, and the temporary storage bottle are moved into the cell from the outside through the inlet gate. Transmission area; in the placing step, the entrance gate is closed, the transmission area is isolated from the outside to form a closed space, a feeding gate and a freezing box gate are opened, so that the transmission area and the packaging area are connected, and the freezing The box enters the packing area from the transmission area through the freezing box gate, the temporary storage bottle and the liquid injection pipeline enter the packing area from the transmission area through the feeding gate, and the feeding gate and the freezing area The box gate is closed again, so that a closed space is formed in the packaging area containing the frozen box and the temporary storage bottle. During the transfer step, the freezer box gate is opened, the freezer box is moved from the repacking area through the freezer box gate to the transfer area, and the freezer box gate is closed again; during the storage step, an exit The gate is opened, the transfer zone clamp and the freezing zone clamp move the freezing box from the transfer zone and move the freezing box through the exit gate, and the exit gate is closed again.

Further, in the aforementioned cell dispensing and storage method, in the preparation step, a feeding area trolley moves the freezing box, the liquid injection line and the temporary storage bottle to the transfer area; in the storage step In the middle, a freezing area trolley moved the freezing box from the vicinity of the transfer area to the freezing cabinet.

10‧‧‧Feeding area

11‧‧‧feed opening

12‧‧‧ Trolley in the loading area

20‧‧‧Transmission area

21‧‧‧Transmission area fixture

22‧‧‧ Take the opening

23‧‧‧Freezing box scanning device

30‧‧‧packing area

31‧‧‧packing station

32‧‧‧packing base

33‧‧‧Rotating column

331‧‧‧Clamp head

34‧‧‧rotating platform

35‧‧‧ Test tube placement

36‧‧‧Fitting zone fixture

40‧‧‧Injection component

41‧‧‧injection nozzle

42‧‧‧injection line

43‧‧‧Filling base

44‧‧‧ Nozzle holder

50‧‧‧freezing zone

51‧‧‧Freezer

52‧‧‧Freezing zone fixture

53‧‧‧Freezer trolley

54‧‧‧Freezing track

60‧‧‧Gate assembly

61‧‧‧Entry gate

62‧‧‧Exit Gate

63‧‧‧feed gate

64‧‧‧Freezer Box Gate

91‧‧‧Freezing Box

92‧‧‧Temporary Bottle

93‧‧‧ test tube

94‧‧‧ cover

Figure 1 is a three-dimensional appearance of this creation.

Figure 2 is a perspective view of a frozen box and a temporary storage bottle.

Figure 3 is a three-dimensional appearance of the frozen box and the temporary storage bottle moved to the transmission area of this creation.

Figures 4 and 5 are exploded views of the components of this creation.

Figures 6 to 13 are the continuous action diagrams of dispensing cells into a test tube.

Fig. 14 is a schematic perspective view of the freezing box moving to the freezer area of the creation.

In the following, with reference to the drawings and the preferred embodiment of this creation, the technical means adopted by this creation to achieve the intended purpose is further explained.

Please refer to FIG. 1 and FIG. 4. The cell dispensing and storage device of the present invention includes a feeding area 10, a transmission area 20, a dispensing area 30, a liquid injection assembly 40, a freezing area 50, and a gate. Component 60.

Please refer to FIG. 3, FIG. 4 and FIG. 5. In this embodiment, the transmission area 20 is a rectangular box, and the four sides of the transmission area 20 sequentially face the feeding area 10, the packing area 30, and the freezing area. 50 and the outside world. In this embodiment, the transmission area 20 and the feeding area 10, the transmission area 20 and the packing area 30, and the transmission area 20 and the freezing area 50 are selectively communicated through the gate assembly 60.

More specifically, the gate assembly 60 includes an entrance gate 61, an exit gate 62, a feeding gate 63, and a freezing box gate 64. The entry gate 61 is disposed between the feeding area 10 and the transmission area 20, and the entrance gate 61 selectively closes the communication relationship between the feed zone 10 and the transfer zone 20; the exit gate 62 is disposed between the transfer zone 20 and the freezing zone 50, and the exit gate 62 selectively closes the transfer zone 20 and the freezing zone 50 The feed gate 63 and the freezer gate 64 are arranged at a distance from each other between the transmission area 20 and the packing area 30, and selectively close the communication relationship between the transmission area 20 and the packing area 30.

When the gates 61, 62, 63, 64 of the gate assembly 60 are in a closed state, the transmission area 20, the packing area 30, and the freezing area 50 each form a closed space, but in other embodiments, the transmission area 20, The space between the packing area 30 and the freezing area 50 can also be a non-closed space. More precisely, even without the gate assembly 60, this creation can also achieve the purpose of "automatic packing", but if these areas 20 The function of sealing between 30, 30, and 50 can further reduce the possibility of cell specimens being contaminated during the packaging process.

In this embodiment, the gate assembly 60 is used to selectively close the communication relationship between the zones, but the user can also achieve the effect of closing the zones by other means.

Please refer to FIG. 1 and FIG. 2. The feeding area 10 has a feeding opening 11 and a feeding area trolley 12. The feeding opening 11 communicates with the outside world, and the user can put objects through the feeding opening 11. Into the loading area 10; the loading area trolley 12 is arranged in the loading area 10, and the loading area trolley 12 can move through and move from the loading area 10 to the transfer area 20. More precisely, in this embodiment, The trolley 12 in the feeding area moves back and forth between the feeding area 10 and the transmission area 20 through the entrance gate 61. In addition, the feeding area 10 can be a biosafety operation cabinet.

Please refer to FIG. 1 to FIG. 4. The transfer area 20 is provided with a transfer area fixture 21, at least one picking opening 22 and a freezing box scanning device 23. The transfer area fixture 21 is movably disposed in the transfer area 20 for more accuracy. In other words, in this embodiment, the transmission area fixture 21 is transmitted through the track (not shown) so that it can move three-dimensionally within the transmission area 20.

At least one picking opening 22 is disposed on the side of the transmission area 20 facing the outside, and in this embodiment, the number of the at least one picking opening 22 is two, and the positions of the two picking openings 22 and the feeding gate are respectively The positions of 63 and the freezing box gate 64 correspond to each other; the freezing box scanning device 23 is disposed in the transfer area 20 and corresponds to the positions of the two picking openings 22.

In the present embodiment, a pair of gloves (not shown) are respectively provided around the two access openings 22, and the gloves abut on the periphery of the access opening 22 and extend into the transmission area 20; in other words, the user can By extending the hand into the glove and inwardly extending into the transmission area 20, the transmission area 20 can also be prevented from communicating with the outside world due to the two access openings 22.

When the user extends his hand from the picking opening 22 into the transfer area 20, the user can pick up a freezer box 91 and move it to the freezer scanning device 23 adjacent to the picking opening 22 for scanning and freezing. The box scanning device 23 can thereby record the data of the frozen box 91.

Please refer to FIG. 6 and FIG. 7. The dispensing area 30 includes a dispensing stand 31, a dispensing base 32, a rotating column 33, a rotating platform 34, a plurality of test tube placement pieces 35, and a dispensing area fixture. 36.

The sub-packing base 32 is arranged on the sub-packing table 31, and the aforementioned freezing box gate 64 corresponds to the position of the sub-packing base 32, and when the freezing box gate 64 is opened, the sub-packing base 32 can be extended to the transmission In the area 20, the transfer area fixture 21 can be moved above the dispensing base 32.

The rotating column 33 is rotatably disposed on the dispensing table 31. At least one clamping head 331 is provided on the rotating column 33, and each of the at least one clamping head 331 can move up and down relative to the rotating column 33. In this embodiment, the The number of the at least one gripping head 331 is two, and the two gripping heads 331 are arranged on the rotating column 33 in an interval, but the number of the gripping heads 331 is not limited thereto.

More precisely, in this embodiment, each of the gripping heads 331 extends horizontally and protrudes out of the rotation post 33, but the relative position of the gripping head 331 and the rotation post 33 is not limited to this.

Please refer to FIG. 7, FIG. 8 and FIG. 9. In addition, in this embodiment, the gripping head 331 is rotatably (rotated) provided on the rotating column 33. The purpose of the gripping head 331 to rotate is because The picking head 331 is used to remove the cover 94 on a test tube 93. The rotating type gripping head 331 can be suitable for screwing the cover 94, but in other embodiments, if the test tube 93 is The lid body 94 is combined with the test tube 93 in a downwardly pressing manner, and the gripping head 331 can also be removed or installed by moving up and down.

The rotation platform 34 is rotatably disposed on the sub-assembly table 31 relative to the rotation column 33. More precisely, in this embodiment, the rotation column 33 extends through the middle of the rotation platform 34 and extends downward and can be connected with the sub-assembly table 31 They are connected in a rotating manner, but the relative position between the rotating column 33 and the rotating platform 34 is not limited to this.

The test tube placing members 35 are arranged on the rotating platform 34 in a circle, and the rotation of the rotating platform 34 enables the test tube placing members 35 to move around the rotating column 33, and the two gripping heads 331 can be moved to each test tube. Above the placement member 35.

The dispensing area fixture 36 is disposed on the dispensing platform 31 and can be moved above the dispensing base 32 and above one of the test tube placement pieces 35. More precisely, in this embodiment, the dispensing area fixture 36 is It is pivotally arranged on the sub-packing table 31, and can be moved from the upper part of the sub-packing base 32 to the top of the test tube placing member 35 closest to the sub-assembly jig 36 by pivoting relative to the sub-packing table 31. The manner in which the zone clamp 36 moves (in this embodiment, pivoting) is not limited to this.

Please refer to FIG. 10 and FIG. 11. The liquid injection assembly 40 is disposed on the sub-assembly table 31. The liquid injection assembly 40 includes a liquid injection nozzle 41, a liquid injection line 42, a liquid injection base 43, and a nozzle holder. 44. The liquid injection base 43 is movably disposed on the sub-packing table 31 relative to the sub-packing table 31. The shower head frame 44 is provided on the liquid injection base 43. The liquid injection head 41 is provided on the shower head frame 44 and located in one of the test tubes Above the placing member 35, more precisely, in this embodiment, the nozzle holder 44 can be rotated relative to the injection base 43, so that the injection nozzle 41 can Move the infusion assembly 40 to selectively move it above one of the test tube placement members 35, but each test tube placement member 35 can also be directly moved below the injection nozzle 41 through the rotation of the rotation platform 34; One end of the liquid line 42 is connected to the liquid injection nozzle 41. In this embodiment, the liquid injection component 40 can attract cells from the temporary storage bottle 92 to the liquid injection nozzle 41 through a peristaltic pump, but the way of attracting cells is not This is the limit.

Further, the position of the aforementioned feed gate 63 corresponds to the position of the liquid injection assembly 40, and the liquid injection base 43 can be moved relative to the dispensing platform 31 from the dispensing area 30 to the transfer via the feed gate 63 Within zone 20.

Please refer to FIG. 6. In this embodiment, the relative positions of the components in the dispensing area 30 are arranged as follows: the rotation column 33 is disposed between the feeding gate 63 and the freezer gate 64, and the dispensing base 32 and The liquid components 40 are respectively disposed on opposite sides of the rotating column 33 and are adjacent to the freezing box gate 64 and the feeding gate 63, respectively, and the dispensing area fixture 36 is disposed adjacent to the dispensing base 32, in other words, when When a test tube placement member 35 is rotated from a position adjacent to the dispensing base 32 to a position adjacent to the liquid injection assembly 40, it is rotated 180 degrees relative to the rotation post 33, but each of the dispensing area 30 The relative position of the components is not limited to this.

Please refer to FIG. 1 and FIG. 4, a freezing cabinet 51, a freezing zone fixture 52, and a freezing zone trolley 53 are provided in the freezing zone 50. The freezing zone fixture 52 is movably disposed in the freezing zone 50 and can be moved to In the freezer cabinet 51, in other words, the position of the freezer clamp 52 and the position of the freezer cabinet 51 selectively correspond.

In this embodiment, the freezing zone fixture 52 is moved through the freezing zone track 54 in the freezing zone 50 and the freezer cabinet 51, but the moving manner of the freezing zone fixture 52 is not limited to this.

In this embodiment, the aforementioned freezing zone trolley 53 can be moved back and forth between the freezing zone 50 and the transfer zone 20 through the exit gate 62, and when the freezing zone trolley 53 moves into the transfer zone 20, the transfer zone The jig 21 can place a freezing box 91 on the freezing zone trolley 53 from the transfer zone 20, and further move the freezing box 91 into the freezing zone 50 by the freezing zone trolley 53.

One end of the freezing zone trolley 53 extends to the connection between the freezing zone 50 and the transfer zone 20. More precisely, in this embodiment, the end of the freezing zone trolley 53 can be moved from the freezing zone 50 into the transfer zone 20 through movement.

In this embodiment, the whole process can be assisted by the trolley 12 in the loading area and the trolley 53 in the freezing area, but it is not limited to this. In other embodiments, the trolleys 12, 53 may not be provided. Instead, the user pushes the freezing box 91 and the temporary storage bottle 92 into the transfer area 20, and then moves the freezing box 91 and the temporary storage bottle 92 together through the transfer area fixture 21 and the freezing area fixture 52.

The following is the use method of the cell dispensing and storage system of this creation, which includes:

Preparation step (S1): Please refer to FIG. 2 and FIG. 6, a freezing box 91, a temporary storage bottle 92, a transfer area 20, a transfer area fixture 21, a dispensing area 30 and a freezing area 50 are prepared. A test tube 93 is placed in the freezing box 91, and a freezing box cover (not shown) is provided above the freezing box 91. A cell specimen (not shown) is provided in the temporary storage bottle 92. In this method, the freezing box 91 There is only a single test tube 93 inside, but the number of test tubes 93 is not limited to this, and it may be a plurality.

The transfer area fixture 21 is disposed in the transfer area 20, and the transfer area 20 is further formed with at least one picking opening 22, and the user can extend into the transfer area 20 through the at least one picking opening 22; inside the dispensing area 30 A dispensing base 32, a rotating column 33, a rotating platform 34, a test tube placement member 35, a dispensing area fixture 36, and a liquid injection assembly 40 are provided. The rotating column 33 is provided with a clamping head 331 for liquid injection The assembly 40 is provided with a liquid injection nozzle 41, a liquid injection line 42, a liquid injection base 43 and a nozzle frame 44. The nozzle frame 44 is disposed on the liquid injection base 43, and the liquid injection nozzle 41 is installed on the nozzle frame 44. One end of the liquid injection line 42 is connected to the liquid injection nozzle 41, and a freezing cabinet 51 and a freezing area jig 52 are provided in the freezing area 50. Because of the sub-packing area 30, the components in the sub-packing area 30, and the transfer area jig 21 And the freezing zone 50 are the same as above, Therefore, its structural features are not repeated here, but it is not limited thereto, and the elements used in this method are not limited to the above-mentioned structures.

Please refer to FIG. 2 and FIG. 3. In the preparation step (S1), first, the freezing box 91, the injection nozzle 41 and the injection line 42 enter the transfer area 20 through an inlet gate 61. More specifically, the freezing box 91 The injection nozzle 41 and the injection line 42 are moved into the transfer area 20 through a feeding area trolley 12. In addition, one end of the injection nozzle 41 and the injection line 42 have been connected to each other in this preparation step, but It is not limited to this.

Placement step (S2): Please refer to FIG. 3, FIG. 4 and FIG. 6. First, the user extends into the transfer area 20 by taking the opening 22, and puts the freezing box 91, the connected liquid injection nozzle 41 and the liquid injection. The pipeline 42 is taken off by the trolley 12 in the feeding area, and after the trolley 12 in the feeding area is moved out of the transmission area 20 again by the entrance gate 61, the entrance gate 61 is closed to isolate the transmission area 20 from the outside to form a closed space.

Then, the feeding gate 63 and the freezing box gate 64 in the transfer area 20 are opened, so that the transferring region 20 and the packing area 30 communicate with each other. The freezing box 91 and the liquid injection line 42 are inserted through the freezing box gate 64 and the feeding gate 63, respectively. Within the packing area 30; more precisely, after the feeding gate 63 and the freezer gate 64 are opened, the packing base 32 and a liquid injection base 43 respectively extend from the packing area 30 to the transfer area 20, and the user After taking the opening 22 into the transfer area 20 to pick up the frozen box 91 and scan and store the data through a frozen box scanning device 23 provided in the transfer area 20, the frozen box 91 is placed on the dispensing base 32. At the same time, the movement of the injection base 43 will move the nozzle holder 44 originally set on the injection base 43 from the dispensing area 30 to the transfer area 20, and then the user will take the opening 22 and extend into the transfer area 20 A liquid injection nozzle 41 connected to the liquid injection line 42 is mounted on the nozzle holder 44.

Next, the transfer area clamp 21 removes the freezing box cover of the freezing box 91, and the dispensing base 32 and the injection base 43 are moved back into the dispensing area 30 again, and enter the dispensing area at the freezing box 91 and the injection line 42 After 30, the feed gate 63 and the freezer gate 64 are closed again; the user then disinfects it through computer control in the transfer area 20 and the packaging area 30. More precisely, this disinfection step is performed by starting the UV-C sterilization lamp. This creation The internal disinfection, but it is not limited to this, users can also perform disinfection through other methods; in addition, the location where the disinfection process is performed includes the feeding area 10, the transmission area 20, and the packing area 30.

After the disinfection is completed, the feeding opening 11 is opened, and the user places the temporary storage bottle 92 sterilized with alcohol on the trolley 12 in the feeding area, and then closes the feeding opening 11; Next, the entrance gate 61 is opened, and the trolley 12 in the feeding area is opened. Then the temporary storage bottle 92 is moved into the transfer area 20. After the user puts the temporary storage bottle 92 into the transfer area 20 by taking the opening 22, the loading area trolley 12 exits the transfer area 20 again through the entrance gate 61 and enters the entrance gate. 61 is closed after the trolley 12 in the loading area is withdrawn.

Next, the feeding gate 63 is opened, and the injection base 43 of the injection assembly 40 moves the interconnected injection nozzle 41 and the injection line 42 together with the nozzle holder 44 into the transfer area 20, and the user reaches into the hand to take The opening 22 connects the other end of the injection line 42 with the temporary storage bottle 92, so that the injection liquid line 42 can suck the cell liquid in the temporary storage bottle 92; then the injection base 43 connects the injection nozzle 41 and the liquid injection line 42 and the temporary storage bottle 92 are moved back to the dispensing area 30 by the feeding gate 63, and then the feeding gate 63 is closed again, so that a closed space is formed in the dispensing area 30 containing the freezing box 91 and the temporary storage bottle 92.

In this method, the moving method of the liquid injection line 42 and the temporary storage bottle 92 from the transfer area 20 to the dispensing area 30 is not limited to the above steps. The liquid injection line 42 and the temporary storage bottle 92 can also be passed through other methods. Moving from the transfer area 20 to the packing area 30.

In addition, in this step, before the freezing box 91 is clamped to the dispensing base 32 by the transfer area clamp 21, the user can also pick up and move the freezing box 91 to the transfer area 20 through the picking opening 22. The freezer scanning device 23 scans and records data of the freezer 91.

Step of opening the tube (S3): Please refer to FIG. 7, FIG. 8 and FIG. 9. The dispensing area fixture 36 moves the test tube 93 placed on the freezing box 91 to the test tube placing member 35 on the rotating platform 34 and rotates the platform. 34 The test tube placing member 35 is circularly moved below the gripping head 331 of the rotating column 33. More precisely, in this embodiment, the rotation angle of the rotating platform 34 in this step is 90 degrees in a counterclockwise rotation direction , But the rotation direction is not limited to this, it can also be clockwise; the rotation platform 34 is rotated 90 degrees and the test tube is placed After 35 moves below the gripping head 331, the gripping head 331 moves down and a cover 94 of the test tube 93 is turned off from the test tube 93. Then, the cover 94 is lifted up and removed from the test tube 93, then The gripping head 331 rises relative to the test tube 93 together with the lid 94.

Liquid injection step (S4): Please refer to FIG. 9, FIG. 10 and FIG. 11, the rotating platform 34 moves the test tube placement member 35 to the injection component 40. More precisely, in this embodiment, the rotating platform 34 is at This step continues to rotate 90 degrees in the counterclockwise direction to move it to the injection nozzle 41. The injection nozzle 41 extracts the cell specimen in the temporary storage bottle 92 and injects it into the test tube 93 without the cover 94. Inside.

Step of closing the tube (S5): Please refer to FIG. 11 and FIG. 12, the rotating column 33 and the gripping head 331 rotate together, and the rotating platform 34 rotates the test tube 93 to the position below the gripping head 331. More precisely, In this step, the rotation column 33 and the gripping head 331 are rotated 180 degrees in the rotation direction, and the rotation platform 34 is rotated 90 degrees in the rotation direction. When the test tube 93 moves below the gripping head 331, the gripping head is 331 moves down and reattaches the lid body 94 to the test tube 93.

Transfer step (S6): Please refer to FIG. 12 and FIG. 13, the rotating platform 34 moves the test tube placing member 35 to be adjacent to the jig 36 in the dispensing area. More precisely, in this step, the rotating platform 34 is along Rotate 90 degrees in the direction of rotation to reach the test tube placement piece 35 adjacent to the filling area fixture 36; then the filling area fixture 36 moves the test tube 93 from the test tube placement piece 35 to the freezing box 91 to complete the liquid filling step; the freezing box gate 64 is opened, the sub-mounting base 32 moves the freezing box 91 into the transfer area 20, and the transferring area clamp 21 covers the freezing box cover back to the freezing box 91, and clamps the freezing box 91 and waits to be placed in the transferring area 20, The dispensing base 32 moves back into the dispensing area 30, and the freezer gate 64 is closed.

Storage step (S7): Please refer to FIG. 14 further, an exit gate 62 is opened to connect the freezing area 50 and the transmission area 20, and then the freezing area trolley 53 enters the transmission area 20 from the exit gate 62, and the transmission area The clamp 21 moves the freezing box 91 from the transfer zone 20 to the freezing zone trolley 53, and then the freezing zone trolley 53 carries the freezing box 91 back to the freezing zone 50, and the exit gate 62 is closed again; the freezing zone trolley 53 further moves the freezing box 91 Move to the place adjacent to the freezer 51, and the freezer clamp 52 holds the freezer 91 It is placed in the freezer 51 to complete the storage purpose. In this step, when the freezer box 91 is moved to the freezer area 50, the exit gate 62 is then closed, so that the freezer area 50 forms a closed space.

Through the above-mentioned structure and method, this creation can achieve the purpose of automated cell aliquoting, which can not only greatly reduce the manpower when aliquoting cell specimens, but also ensure that the entire process of aliquoting cells is in a closed space and has no outside. Interference, so it can effectively prevent bacteria or other impurities from entering the test tube to contaminate the cell specimen, making the cell specimen unusable.

The above description is only the preferred embodiment of the creation, and does not limit the creation in any form. Although the creation has been disclosed as above with the preferred embodiment, it is not intended to limit the creation. Generally, a knowledgeable person can use the technical content disclosed above to make a few changes or modifications as equivalent equivalent changes without departing from the scope of the technical solution of the creation. Anyone who does not depart from the content of the technical solution of the creation, Any simple modifications, equivalent changes, and modifications made to the above embodiments according to the technical essence of this creation still fall within the scope of this creative technical solution.

Claims (9)

A cell dispensing and storage device includes a feeding area, which has a feeding opening, which is connected to the outside; a transmission area, which is selectively connected to the feeding area, and the transmission area. A transmission area fixture is provided, and the transmission area fixture is movably disposed in the transmission area. At least one picking opening is further formed on a wall surface of the transmission area. A dispensing area is selectively connected to the transmission area. A sub-packing station is provided in the sub-packing area; a sub-packing base is movably arranged on the sub-packing station, and the sub-packing base can be moved to the transmission area; a rotating column, It is rotatably arranged on the dispensing platform, and at least one gripping head is provided on the rotating column, which can move up and down relative to the rotating column; a rotating platform is rotatably provided on the separating column relative to the rotating column. Mounted on the table; a plurality of test tube placing pieces are arranged on the rotating platform in a circle, and the rotation of the rotating platform enables the test tube placing pieces to move around the rotating column, and the at least one gripping head can be moved to each of the test tube placing Above the piece; a dispensing area fixture It is arranged on the dispensing platform and can be moved above the dispensing base and above one of the test tube placement pieces; a liquid injection component is disposed on the dispensing platform, and the liquid injection component includes a Nozzle frame, a liquid injection nozzle and a liquid injection line, the nozzle frame is arranged on the dispensing platform, the liquid injection nozzle is installed on the nozzle frame and is located above one of the test tube placement parts, and the liquid injection line One end is connected to the liquid injection nozzle; a freezing area is selectively communicated with the transmission area, and a freezing cabinet and a freezing area fixture are provided in the freezing area; the freezing area fixture is movably disposed in the freezing area Freezing zone and can be moved into the freezer. The cell dispensing and storage device according to claim 1, further comprising a loading area trolley, which is arranged in the loading area, and the loading area trolley can be moved to the transfer area; a freezing area trolley It is set in the freezing zone, and the freezing zone trolley can be moved to the transmission zone. The cell dispensing and storage device according to claim 1 or 2, further comprising a gate assembly, the gate assembly includes an entrance gate, which is arranged between the feeding area and the transmission area, and the entrance gate is selected The connection between the feeding area and the transmission area is closed; an exit gate is arranged between the transmission area and the freezing area, and the exit gate selectively closes the transmission area and the freezing area A feed gate, the feed gate is disposed between the transfer area and the repacking area, and is adjacent to the feed area, and the feed gate selectively closes between the transfer area and the refill area A frozen box gate, which is arranged between the transmission area and the packaging area and is adjacent to the freezing area, and the freezing box gate selectively closes the transmission area and the packaging area Connected relationship. The cell dispensing and storage device according to claim 1 or 2, wherein each of the at least one gripping head is capable of rotating relative to the rotating column. The cell dispensing and storage device according to claim 1 or 2, wherein a freezing box scanning device is further disposed in the transmission area, which is disposed adjacent to the at least one picking opening. A cell dispensing and storage method includes the following steps: a preparation step: preparing a temporary storage bottle, a freezing box, a freezing box lid, a transfer area, a transfer area fixture, a dispensing area, and a freezing area, the A cell specimen is placed in the temporary storage bottle, a test tube is arranged in the freezing box, and the freezing box cover is arranged on the freezing box. The transfer zone fixture is set in the transfer zone, and a sub-zone A mounting base, a sub-assembly area fixture, a rotating column, a rotating platform, a test tube placement piece, and a liquid injection assembly; the rotating column is provided with a gripping head; the liquid injection assembly includes a nozzle holder, a liquid injection Nozzle and a liquid injection line; a freezing cabinet and a freezing zone fixture are provided in the freezing zone; placing steps: the dispensing base is moved from the dispensing zone to the transfer zone, and the freezing box is placed on the dispensing base Above, the transfer area clamp lifts the freezer lid from the freezer, the dispensing base moves the freezer from the transfer area to the dispensing area, and the dispensing area fixture moves the freezer inside The test tube is moved into the test tube placement part on the rotating platform, and the nozzle holder is arranged In the dispensing area, the liquid injection nozzle is arranged on the nozzle holder, the temporary storage bottle and the liquid injection pipeline are placed in the liquid injection module, and the opposite ends of the liquid injection pipeline are respectively connected with the liquid injection nozzle and The temporary storage bottle is connected; the tube opening step: the rotating platform circularly moves the test tube placing piece below the gripping head of the rotating column, the gripping head is moved down and a cover of the test tube is removed from the test tube After the upper part is removed, the liquid injection step: the rotating platform moves the test tube placement part to the liquid injection component, the liquid injection nozzle extracts the cell specimen in the temporary storage bottle and injects the test tube; the tube closing step: The rotating column rotates with the gripping head, and the rotating platform moves the test tube below the gripping head, and the gripping head moves down and the cover is mounted on the test tube; the transmission step: the The rotating platform moves the test tube placement piece to a position adjacent to the fixture of the dispensing area, and the dispensing area fixture clamps the test tube from the test tube placement piece into the freezing box; the storage step: the dispensing base The freezer box is moved to the transfer area, and the transfer area clamp covers the freezer cover The cartridge is provided above freezing, and the freezing cassette followed by the removal of the dispensed base region and the transmission region moves to the freezing, the freezing zone the tube clamp to move the refrigerator cabinet. The cell dispensing and storage method according to claim 6, wherein, from the placing step to the tube opening step, the rotating platform rotates the test tube placing member 90 degrees relative to the rotating column in a rotating direction; From the step to the liquid injection step, the rotary platform rotates the test tube placing member 90 degrees relative to the rotating column in the rotation direction; from the liquid injection step to the tube closing step, the rotating platform rotates the test tube placing member relative to the rotation The column is rotated 90 degrees in the direction of rotation; from the step of closing the tube to the step of transfer, the rotating platform rotates the test tube placement piece 90 degrees in the direction of rotation with respect to the rotating column. The cell dispensing and storage method according to claim 6 or 7, wherein in the preparation step, an entrance gate of the transfer area is opened, and the freezing box, the injection line and the temporary storage bottle pass through the entrance from the outside. The gate is moved into the transmission area; in the placing step, the entrance gate is closed, so that the transmission area is isolated from the outside to form a closed space, a feeding gate and a freezing box gate are opened, so that the transmission area and the packaging area are connected. , The freezing box enters the packing area from the transmission area through the freezing box gate, the temporary storage bottle and the liquid injection pipeline enter the packing area from the transmission area through the feeding gate, and the feeding gate And the freezing box gate is closed again, so that a closed space is formed in the packaging area containing the freezing box and the temporary storage bottle; in the transferring step, the freezing box gate is opened, and the freezing box is filled by the packing The area is moved to the transfer area through the freezing box gate, and the freezing box gate is closed again. In the storage step, an exit gate is opened, and the transfer area fixture and the freezing area fixture move the freezing box out of the transfer area. And move through the exit gate To the frozen area, and the exit gates close again. The cell dispensing and storage method according to claim 6 or 7, wherein in the preparation step, a feeding area trolley moves the freezing box, the liquid injection line and the temporary storage bottle into the transfer area; In the storing step, a freezing area trolley moves the freezing box from the vicinity of the transfer area to the freezing cabinet.