TWI657747B - Peach extract and method for producing the same - Google Patents

Abstract

An object of the present invention is to provide a peach extract having active oxygen scavenging energy derived from peach, and a method for producing the peach extract having excellent safety.

It is a peach raw material obtained from sparse peaches (young peaches), ripe peaches, or sparse peaches or ripe peaches by removing the seeds and fruit juice, and containing fiber-containing peach processing residues, after passing through ethanol or an ethanol / hexane mixture Peach extract with active oxygen scavenging energy obtained by one-stage extraction with hexane / water, and a peach extract containing ceramide (sphingomyelin) and polyphenols , And its manufacturing method. The peach extract prepared by the present invention can be used in food, cosmetics or quasi-drugs, and processed products thereof.

Description

Peach extract and manufacturing method thereof

The present invention relates to a peach extract having active oxygen scavenging ability and containing sphingomyelin (neuraminyl component), and a method for producing the same.

Recently, research on materials derived from natural ingredients in terms of safety has proposed various plant extracts having effective physiological activity.

The inventors of this case are also concerned about vegetable oils and fats, and in particular the usable trace ingredients contained in the by-products produced during the production of edible rice bran oil using rice bran as a raw material. The results of the review have established the extraction and manufacturing method of rice-derived nerve ceramide. It can provide rice-derived ceramide (sphingomyelin) of natural origin.

The rice-derived neuraminamine provided by the present inventors is a neuraminamine derived from natural materials, which has high safety and extremely high purity. Therefore, it has been widely used as food, cosmetics or quasi drugs, and the And other processed materials.

Neuraminamine is a kind of sphingomyelin, which is a long-chain amine. A group of compounds in which ceramide and fatty acids are bonded via amidine. This neuraminamine is known to exist in cell membranes at high concentrations, and in particular, to be the main component of interkeratinocyte lipids of the skin, and to serve as a barrier function to prevent moisture from evaporating from the skin.

However, it has been reported that as age increases, neuraminamine in the keratinocyte lipids is also reduced, which reduces the ability to moisturize, which can cause dry skin, wrinkles, or rough skin.

At the same time, not only the skin is aging, but the main causes of dry skin and rough skin due to atopic dermatitis are reported as the deficiency of neuraminamine. That is, the report points out that the characteristic of dry skin in atopic dermatitis is that the original neuraminamine is less. As the barrier function of the skin is reduced, moisture is easily lost from the skin to the outside, making the skin rough and dry As a result, antigens and chemicals can easily enter the skin, resulting in allergic reactions, showing various symptoms such as itching and inflammation.

Therefore, the content of ceramide in the skin has become one of the indicators of skin health. Generally, it is pointed out that the supplement of inadequate ceramide has the moisturizing effect of the skin, or is effective for whitening, so that ceramide becomes the raw material of cosmetics At the same time, demand has increased, and at the same time, functional foods have been widely used to alleviate the symptoms of atopic skin inflammation.

In addition, Neuramin is also known to have activating immune effects and anti-tumor effects, so its demand will continue to increase in the future.

In addition, various studies have been conducted on neural amine components from plants such as various fruits and vegetables (Patent Document 1). Among them, because peach is said to contain a large amount of ceramides, Technique for taking Neuraminamine (Patent Document 2).

In terms of the ingredients contained in peach, not only the extraction of ceramide, but also the physiological activity of the peach fruit itself. It has also been reported that the polyphenols contained in peach fruit and the active oxygen scavenging energy of peach itself have been reviewed (non- (Patent Documents 1 to 3).

The above-mentioned Patent Document 2 describes the extraction of ceramide from peach. The extraction method uses a chloroform / methanol mixture as the extraction solvent to prepare the glucosylceramide profile. However, chloroform and methanol used as the extraction solvent are considered. In terms of toxicity, it is not ideal from the aspect of safety.

Therefore, it is required to review the method of extracting neural crestamine from peach to ensure safety.

So far, the proposed technology for extracting / manufacturing neural cramine from plant components such as various fruits and vegetables is known. For example, grapes, cherries, wheat, rice, soybeans, and other plant materials are extracted through an alcohol-based solvent (such as ethanol). Method (Patent Document 1); a method in which camellia seed oil residue is degreased / pretreated with hexane and then extracted with ethanol (Patent Document 3); rice bran is selected from the group consisting of: water, ethanol, acetone, and hexane. A method of extracting more than one mixed solvent (Patent Document 4); a method of extracting plant materials such as wheat, soybean, corn, and rice bran with alkaline ethanol solvent, and then extracting the mixed solvent with hexane, acetone, and water (patent) Documents 5 and 6).

The present inventors have attempted to extract peaches by the method of extracting neural crestamine components described in each of the above-mentioned patent documents, but they have not been satisfactory. Therefore, in view of the current situation, we reviewed the efficient and safe extraction of neural amines from peaches. Extraction with alcohol or ethanol / hexane mixed solution, and then extracting the obtained extract with hexane / water Partitioning Extraction, can efficiently obtain active oxygen scavenging energy and sphingomyelin-containing lipid (Nervamine component) peach extract, then completed the present invention.

[Prior technical literature] Patent literature

[Patent Document 1] Patent Publication No. 4108069

[Patent Document 2] Patent Publication No. 4723630

[Patent Document 3] Japanese Patent Laid-Open No. 2007-308424

[Patent Document 4] Japanese Patent Application Laid-Open No. 11-279586

[Patent Document 5] Japanese Patent Laid-Open No. 2002-30093

[Patent Document 6] Japanese Patent Laid-Open No. 2006-232699

Non-patent literature

[Non-Patent Document 1] Yamanashi Industrial Technology Center Research Report No. 22, p. 59 (2008)

[Non-Patent Document 2] Okayama Prefecture Agricultural Research Report No. 2, p. 21 (2011)

[Non-Patent Document 3] Journal of Nutrition Vol.70, No.3, p.207 (2012)

Therefore, the subject of the present invention is to provide a method for efficiently and safely extracting a peach extract having active oxygen scavenging energy from peach, and to provide a method for producing active oxygen scavenging by the manufacturing method. A peach extract containing sphingomyelin (neuraminyl component).

In order to solve this problem, the peach extract of the basic embodiment of the present invention is characterized in that: a peach processing residue containing fiber components after removing seeds and fruit juice from sparse peach, ripe peach, or sparse peach or ripe peach After being extracted with ethanol or a mixed solution of ethanol / hexane, the extract is then extracted with hexane / water in stages to obtain active oxygen scavenging energy and sphingomyelin (neuraminamine component).

More specifically, in the peach extract of the present invention, the above-mentioned active oxygen scavenging energy is DPPH (diphenyl picryl hydrazyl) activity.

To be more specific, the peach extract of the present invention has a DPPH activity of 5% or more and a neural amine content of 0.5% or more.

At the same time, other aspects of the present invention are a method for producing a peach extract, which is a method for producing a peach extract having active oxygen scavenging energy and containing a sphingomyelin (neuraminyl component). Specifically, the method The fruit processing peach, the ripe peach, or the peach or ripe peach is removed from the seeds and fruit juice, containing the fiber component of the peach processing residue, and then extracted with ethanol, or an ethanol / hexane mixture, and the resulting extract is passed through Alkane / water staged extraction.

More specifically, the present invention is a peach extract and a method for producing the same, wherein the volume ratio of the hexane and water used for the staged extraction of the extraction liquid and the extraction liquid of the extraction residue of the peach processing residue is 1: 0.5: 0.5 to 1: 2: 2.

Meanwhile, the present invention is a food, a cosmetic or a quasi-drug containing the above-mentioned peach extract, and processed products thereof.

More specifically, the present invention is a powder, a food, a cosmetic, or a quasi-drug in the form of an aqueous solution obtained by mixing the above-mentioned extract with a cyclodextrin, and processed products thereof.

According to the present invention, a peach extract having active oxygen scavenging energy can be efficiently obtained from a peach raw material by a method with excellent safety and a relatively simple method.

The method for producing a peach extract provided by the present invention is an extraction liquid obtained by using ethanol or an ethanol / hexane mixed liquid on a peach raw material, which is a so-called primary extraction liquid, and then subjected to hexane / water segment extraction. In point of view, this is a special manufacturing method that has not been reviewed so far.

As a result, the produced peach extract can contain ceramide (sphingomyelin), polyphenols, and glycosides, and has active oxygen scavenging ability and high safety. It can be used as food, cosmetics, or quasi drugs, and These processed products have extremely high availability.

As described above, the peach extract of the present invention is characterized in that the peach raw material containing peach processing residues containing fiber components obtained by removing seeds and fruit juice from sparse peach, ripe peach, or sparse peach or ripe peach is subjected to Ethanol, or ethanol / hexane mixed liquid extraction, and then the extract is obtained by hexane / water segmented extraction has active oxygen scavenging energy and contains sphingomyelin lipid (neuraminyl component).

The raw fruit sparse peach refers to the sparse fruit removed in the peach production process Peach (young peach); ripe peach refers to the peach in the peach suitable for shipment to the market.

Moreover, the peach processing residue refers to the processing residue of the peach containing fiber components obtained by removing seeds and fruit juice from sparse peach (young peach) or ripe peach.

The amount of peaches harvested in Japan is about 150,000 tons per year, and the sparse peaches (young peaches) produced during the harvest process are said to be about 15,000 tons of 10% of the peach harvest. Only a very small part of this sparse fruit peach is processed as edible pickles, etc., and most of it is directly spread on the peach farm (peach garden) as a fertilizer without treatment, so there is no effective utilization method.

On the other hand, processing companies such as peach juice (peach juice) can produce by-products of peach processing residues of about 1,500 tons in the middle of the year, but they are not effectively used and discarded.

The present invention is very special because it has the point that the peach fruit or the peach processing residues that have been discarded that cannot be effectively used are made into a peach extract with a special combination of extraction solvents, and this by-product can be effectively used.

As a raw material for peaches, when using sparse peaches (young peaches) or ripe peaches, the peaches containing flesh and peel are removed from the seeds of this type of sparse peaches (young peaches) or ripe peaches. Those that need to be frozen in advance, and each are finely pulverized by a pulverizer or the like, and dried by warm air drying or reduced pressure drying, and used as raw materials for extraction.

At the same time, as a raw material for peaches, when using peach processing residues containing sparse peaches or ripe peaches to remove fiber and seeds, the peach can be processed as a by-product of peach juice and other processing steps. The residue (so-called peach pulp) is dried in the same manner as described above, and then used as a raw material for extraction.

Of course, peach processing residues produced as by-products from processing steps such as peach juice may also be used as raw materials for extraction without drying.

The primary extraction solvent used in the present invention to produce peach extracts from these peach materials is characterized by using ethanol alone or using a mixed solution of ethanol / hexane.

The extraction solvent of peach disclosed in Patent Document 2 is a solvent in which chloroform / methanol is mixed at a volume ratio of 1: 1. Therefore, in the obtained glycoside neural crestamine extract (extract), chloroform or methanol having strong toxicity is included. Residual concerns may not be good.

In contrast, in the present invention, ethanol and hexane used in food processing and fat processing are used, and therefore the obtained extract has extremely high safety.

One-time extraction solvent, in addition to ethanol or ethanol / hexane, methanol, propanol, isopropanol, butanol, isobutanol, and other lower alcohols that can be mixed with water, acetone alone, etc. A mixed liquid between solvents, or a mixed liquid of such solvents and hexane.

The use amount of the extraction solvent with respect to the peach raw material which is the raw material for extraction is not particularly limited, but it is preferably used in a conversion of about 0.1 to 1 L with respect to 100 g of the peach fine powder or the peach processing residue which belongs to the raw material.

At the same time, when extracting with a mixed liquid of ethanol / hexane, the mixing ratio is: 1 to 5 capacity of ethanol is better than 1 to 3 capacity of ethanol relative to 1 capacity of hexane.

The extraction temperature and time in this case are not particularly limited, but it is preferably carried out at a temperature from room temperature to about 80 ° C. for about 0.1 to 24 hours.

As described above, in the extraction step in the method for producing a peach extract of the present invention, one extraction can be performed with ethanol alone or with a mixed solution of ethanol / hexane. When extracting with ethanol alone, aqueous ethanol can also be used.

The method for producing a peach extract according to the present invention is a so-called one-time extraction solution which is extracted with ethanol alone or with a mixed solution of ethanol / hexane, and then subjected to segment extraction through hexane / water to obtain the hexane solution layer. Purpose: Peach extract with active oxygen scavenging ability and sphingomyelin (neuraminyl component).

After review by the inventors, it can be known that the volume ratio of hexane to water used in the extraction liquid in a single extraction and the stepwise extraction of the extraction liquid is preferably 1: 0.5: 0.5 to 1: 2: 2 ( (Refer to the embodiment described later).

Therefore, when the volume of the extraction liquid (ethanol alone or ethanol / hexane mixed liquid) used in one extraction is 1 volume, the hexane and The capacity ratio of water can be appropriately selected within the range of 0.5 to 2 capacity of hexane and 0.5 to 2 capacity of water.

When the extraction ratio and the hexane / water stepwise extraction are performed at this capacity ratio, the separation degree and the content of neural amines in the staged extraction can be improved.

It is generally known that when the organic layer and the water layer are in an emulsified state without being separated, the emulsification can be eliminated by adding salt, heating / cooling, or filtering using an inactive carrier such as diatomaceous earth. Of course, in the present invention, this type of method has been reviewed and adopted, and it has been confirmed that under the mixing ratio in the emulsified state, it is processed through salting out, heating / cooling, or filtration using inactive carriers such as diatomaceous earth. Can eliminate the situation of emulsification.

However, in the process of adding salt and filtering with diatomaceous earth, extraction It will inevitably be mixed with salts and carriers. At the same time, methods such as heating / cooling not only have tedious processing steps such as temperature management and time management, but also often cannot guarantee that they can be separated.

On the other hand, in the solvent ratio of the present invention, after mixing, it can be efficiently separated as long as it is left to stand.

This stepwise extraction with hexane / water with respect to the primary extraction solution, and its solvent ratio are novel findings based on the review results of the present inventors, which is very special.

Furthermore, the hexane solution layer obtained by the hexane / water segmented extraction can also be subjected to, for example, activated carbon treatment and chromatographic treatment, if necessary, to further improve the refined system of the extract.

At the same time, in the extraction technique of the method for producing a peach extract of the present invention, a young crushed peach, a ripe peach, or a young peach or a ripe peach, which has been removed from seeds and juice, is finely pulverized and dried with a fiber-containing peach processing residue. It can also be degreased with hexane in advance, and the lipid content in the peach extract can be reduced by the degreasing treatment. As a result, the content of neural amine can be increased.

At the same time, in the above-mentioned one-time extraction operation, the extraction operation can also be performed under moderate alkaline conditions, but the extraction operation under the alkaline conditions is not necessary.

Furthermore, it is also known that when using an extraction solvent alone for extraction with an ethanol solvent, the polyphenol content in the extract can be made high.

Therefore, the extraction conditions can be appropriately selected according to the use of the ingredients contained in the target extract. From this point, the production of the peach extract of the present invention The method is extremely special.

The extract obtained by the above-mentioned extraction treatment is concentrated under reduced pressure, and dried under reduced pressure to become a solid, thereby preparing the peach extract of the present invention.

The prepared peach extract contains ceramide (sphingomyelin), polyphenols, and glycosides. It contains active oxygen scavenging ability due to its polyphenol and other antioxidant active substances. The content is also high. The so-called "Peach Nervamine" can be effectively used in food, cosmetics or quasi drugs, and processed products of these.

In particular, the peach extract provided by the present invention has an active oxygen scavenging energy DPPH (diphenyl bitter hydrazine) activity of 5% or more, and a neuroniamine content of 0.5% or more.

More specifically, the peach extract can be used, for example, in a powdery state mixed with, for example, cyclodextrin, or dissolved in an aqueous solution to form a solution, and used in food, cosmetics, or quasi-drugs, and the like. Product.

[Example]

Hereinafter, the specific production method of the peach extract of the present invention will be described in more detail with reference to the examples, but the present invention is not limited to these examples, and it does not depart from the technical idea of the present invention. Various changes can be made within the scope.

Test Example 1: Determination of Neuraminamine Content

The measurement of the ceramide content (purity) of the peach extract in the following examples can be performed as follows.

1. The measurement sample is dissolved in chlorine so that the peach extract becomes 20 mg / mL. A sample solution was prepared in a mixture of imitation / methanol (2: 1 v / v).

2. The standard product uses a standard product from American Matreya Company (a refined product from soybeans) so that it becomes 2 mg / mL, and is dissolved in a chloroform / methanol (2: 1 v / v) mixed solution, and produced according to Sequential dilution series to make a standard solution.

3. On a TLC chromatography plate (Analtech, Silica Gel GF, Scored 10 × 20cm, 250 μm), spot each with 1 μL of the sample solution and the standard solution, and then develop the solvent (chloroform: methanol: acetic acid: water = 20: 3.5: 2.3: 0.7 v / v / v / v). After development, the chromatography plate was dried at room temperature, sprayed with 50% sulfuric acid, and heated at 150 ° C for 6 minutes to develop a color.

The Neuramin content (purity) in the peach extract was determined by measuring the color point concentration on the chromatography plate using a photodensitometer (manufactured by Atto Corporation, CS Analyzer), and then calculating using a standard curve prepared from a standard solution.

Test Example 2: Determination of DPPH radical elimination activity

In the following examples, the measurement of the DPPH (diphenylpictrazine) radical elimination activity was performed as follows.

1. The measurement samples were adjusted to 2 mg / mL with ethanol.

2. DPPH was adjusted to 0.4 mM with ethanol.

3. Adjust the measurement sample solution as follows.

(1) Sample group

1mL of DPPH solution and 1mL of ethanol in 2mL of 2mg / mL sample solution

(2) Sample blank group

Mix 2mL of ethanol in 2mL of 2mg / mL sample solution

(3) Control group

Mix 3mL of ethanol with 1mL of DPPH solution

(4) Blank control group

4mL of ethanol

4. After the above measurement sample solution is prepared, it is shielded from light for 20 minutes to react. After the reaction, the absorbance (OD) at 571 nm was measured with a spectrophotometer.

The radical elimination rate (%) is determined by the following formula.

Free radical elimination rate (%) = [1- (OD of sample group-OD of blank sample group) ÷ (OD of control group-OD of blank control group) × 100

Test example 3: determination of total polyphenol content

Meanwhile, the measurement of the total polyphenol content in the following examples was performed as follows.

1. The measurement sample was adjusted to 10 mg / mL with 80% ethanol, and then diluted 10 times with distilled water to 1.0 mg / mL as the measurement sample.

2. The gallic acid of the standard product is prepared as follows. 28ppm, 14ppm, 7ppm, 33.5ppm, 1.75ppm

3. Make a 10% sodium carbonate aqueous solution.

4. After that, make a 5-fold diluted phenol reagent with purified water.

5. To 2 mL of each measurement sample, add 2 mL of a 5-fold diluted phenol reagent and 2 mL of a 10% sodium carbonate aqueous solution, and stir for 1 hour to react.

6. The reaction solution was filtered, and the absorbance at 760 nm was measured.

7. Prepare a gallic acid standard curve and find the total polyphenol (total PP) content (mg / g).

Reference Example 1: Manufacture of an extract from a peach processing residue separately extracted with ethanol

To 10 g of the dried peach processing residue, 20 mL of 95% ethanol was added, and extraction was performed at 42 ° C. with stirring for 30 minutes. The extract was then filtered under reduced pressure to obtain an extract. On the other hand, the extraction residue was subjected to the same extraction treatment again to obtain an extract. The two extracts were combined and concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 1.17 g of an orange solid.

The obtained extract was measured for Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 0.7%

DPPH activity: 88.8%

Total polyphenol content: 19.0mg / g

Reference Example 2: Production of an extract from a peach processing residue separately extracted with hexane

To 60 g of the dried peach processing residue, 240 mL of hexane was added, and the mixture was extracted (defatted) at 60 ° C. with stirring for 2 hours. The extract was then filtered under reduced pressure and discharged with 60 mL of hexane. The obtained filtrate was concentrated under reduced pressure, and the residue was dried under reduced pressure to obtain 0.3 g of a golden yellow syrup.

The obtained extract was measured for Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 0.0%

DPPH activity: 24.6%

Total polyphenol content: 2.7mg / g

Moreover, this extract is almost entirely lipid.

Example 1: Extraction from sparse peach (young peach) <Extraction solvent: Separate extraction with ethanol, hexane / water, and alkali treatment>

Frozen sparse peaches (young peaches) were thawed in a microwave oven for 15 minutes, finely pulverized with a pulverizer, and dried. Subsequently, 300 mL of 95% ethanol (containing 9.6 g of sodium hydroxide) and 37.5 mL of purified water were added to 50 g of the powder, and extracted at 42 ° C. with stirring for 30 minutes. The extract was filtered under reduced pressure, and 300 mL of hexane and 150 mL of purified water were added to the filtrate, stirred, and extracted in stages. Thereafter, the hexane layer was recovered, the solvent was concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 160.7 mg of a green solid.

The obtained extract was measured for Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 1.8%

DPPH activity: 6.5%

Total polyphenol content: 0.4mg / g

Example 2: Extraction from sparse peach (young peach) <Extraction solvent: Separate extraction with ethanol, hexane / water without alkali treatment>

The frozen sparse peach (young peach) was thawed for 15 minutes in a microwave oven, pulverized finely with a pulverizer, and dried. Thereafter, 300 mL of 95% ethanol and 37.5 mL of purified water were added to 50 g of the powder, and extracted at 42 ° C. for 30 minutes with stirring for 30 minutes. Then, the extract was filtered under reduced pressure, and 300 mL of hexane and 150 mL of purified water were added to the filtrate, stirred, and extracted in stages. after that The hexane layer was recovered, the solvent was concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 486.0 mg of a green solid.

The obtained extract was measured for Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 2.2%

DPPH activity: 22.5%

Total polyphenol content: 3.4mg / g

Example 3: Manufacturing extracts from peach processing residues <Extraction solvent after degreasing: Separate extraction with ethanol and hexane / water>

First, the pressed residue (peach processing residue) after the production of the peach juice was dried, and then 800 mL of hexane was added to 200 g of the peach processing residue, and the mixture was stirred at 70 ° C. for 4 hours for degreasing treatment. The peach processing residue after the degreasing treatment was filtered under reduced pressure and recovered. Then, 800 mL of 95% ethanol was added to the obtained peach-processed residue after the degreasing treatment, and extraction was performed at 70 ° C. for 60 minutes, followed by filtering under reduced pressure to obtain an extract. The extraction residue was treated again to obtain an extract.

After that, the two extracts were combined, adjusted to a capacity of 1600 mL with 95% ethanol, and then 1600 mL of hexane was added, followed by stirring at 70 ° C. for 60 minutes. After that, 1600 mL of purified water was added again, and the extraction was performed at 70 ° C. for 60 minutes in stages. Thereafter, the hexane layer was recovered, and the solvent was concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 4.36 g of a brown solid.

The obtained extract was then used to determine the Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, The results are as follows.

Nervamine content (purity): 8.8%

DPPH activity: 72.7%

Total polyphenol content: 7.6mg / g

Example 4: Extraction from Peach Processing Residue <Extraction solvent: Separate extraction with ethanol, hexane / water, and alkali treatment>

200 g of 95% ethanol (containing 6.4 g of sodium hydroxide) and 25 mL of purified water were added to 100 g of undried peach processing residues, and extracted at 42 ° C. with stirring for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, and the extraction residue was treated again to obtain an extract. The two extracts were combined, 400 mL of hexane and 200 mL of purified water were added, and the mixture was stirred for 1 hour and extracted in stages. Then, the hexane layer was recovered, and the solvent was concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 131.3 mg of an orange solid.

The obtained extract was measured for Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 10.7%

DPPH activity: 22.2%

Total polyphenol content: 4.3mg / g

Example 5: Extraction from Peach Processing Residue <Extraction solvent: Separate extraction with ethanol, hexane / water without alkali treatment>

Add 100 mL of 95% ethanol to 100 g of undried peach processing residue And 25 mL of purified water, and extracted at 42 ° C with stirring for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, and the extraction residue was treated again to obtain an extract. The two extracts were combined, 400 mL of hexane and 200 mL of purified water were added, and the mixture was stirred for 1 hour and extracted in stages. Thereafter, the hexane layer was recovered, and the solvent was concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 172.1 mg of an orange solid.

The obtained extract was measured for Neuramin content (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 11.7%

DPPH activity: 61.9%

Total polyphenol content: 4.4mg / g

Example 6: Extraction from Peach Processing Residue <Extraction solvent: ethanol / hexane mixed solution hexane / water staged extraction>

To 100 g of undried peach processing residues, 200 mL of 95% ethanol, 100 mL of hexane, and 25 mL of purified water were added, and extracted at 42 ° C. with stirring for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, and 100 mL of hexane and 100 mL of purified water were added to the filtrate, and the mixture was stirred at 42 ° C. for 1 hour, and then extracted with hexane / water in stages. Then, the hexane layer was recovered, the solvent was concentrated under reduced pressure in a distillation apparatus, and the residue was dried under reduced pressure at 60 ° C to obtain 177.8 mg of an orange solid.

The obtained extract was measured for the content of neural cramine (purity), DPPH activity, and total polyphenol content in accordance with Test Examples 1, 2, and 3, and the results were as follows.

Nervamine content (purity): 10.7%

DPPH activity: 21.4%

Total polyphenol content: 1.4mg / g

Example 7: Extraction from Peach Processing Residue <Extraction solvent: Separate extraction with ethanol, hexane / water>

200 mL of 95% ethanol was added to 50 g of the dried peach processing residue, and extracted at 42 ° C. with stirring for 30 minutes. The extract was filtered under reduced pressure and discharged with 50 mL of 95% ethanol. The residue was extracted again in the same manner, and filtered off under reduced pressure and discharged. Then, 150 mL of hexane was added to the combined filtrate (150 mL), and the mixture was stirred for 30 minutes. Then, 150 mL of distilled water was added, and the mixture was stirred for 30 minutes to perform hexane / water stepwise extraction. Thereafter, the hexane layer was recovered and concentrated under reduced pressure, and the residue was dried under reduced pressure. The obtained extract was 3.85 g of an orange syrupy substance.

With respect to the obtained extract, according to the above-mentioned Test Examples 1, 2 and 3, the Neuramin content (purity), DPPH activity, and total polyphenol content were measured. The results are as follows.

Nervamine content (purity): 2.4%

DPPH activity: 36.5%

Total polyphenol content: 29.2mg / g

Example 8: Review of hexane / water staged extraction conditions of peach raw material extract

Degrease the dried peach processing residues first, and then review the hexane / water segmentation based on the solvent ratio (primary extraction solution: hexane: water) described in Table 1 below for the primary extraction solution extracted with 95% ethanol. Extracted solvent capacity ratio.

First add the extract once to the test tube, then add hexane and shake vigorously for 10 seconds, then add water, and shake vigorously for another 10 seconds, then let it stand and review the separation state of the solution.

At the same time, the content of neuraminine was compared with respect to the hexane layer (supernatant layer) and thin layer chromatography (TLC) in terms of sensitivity.

Then, the content of Neuramin during the extraction treatment with one extraction solution: hexane: water = 1: 1: 1 is 100%, and the amount of Neuramin in each mixing ratio is compared.

The results are shown in Table 1 below.

Note 1: The meaning of resolution is as follows.

：: Separation is possible within 10 minutes, and the separation is extremely good.

○: No separation time is required, and good separation can be achieved within 30 minutes.

△: It takes more than 30 minutes to separate, and the intermediate layer is visible, but it is roughly separable.

×: It is in an emulsified state and cannot be separated.

Judging from the above results, in the present invention, the hexane and water used in the one-stage extraction of the hexane and water in the one-stage extraction of the solvent from the peach raw material followed by the hexane / water stage extraction are used. The capacity ratio can be well understood. When the ratio is 1: 0.5: 0.5 to 1: 2: 2, the resolution and the recovered amount of ceramide are good.

As can be seen from the examples shown above, the present invention can effectively obtain a peach extract having active oxygen scavenging energy by using a special extraction method from a peach raw material.

In addition, the obtained extract not only has active oxygen scavenging energy, but also contains ceramide, and its extraction solvent is also highly safe in the point of using ethanol and hexane. Through the extraction technology corresponding to each purpose, the point that can prepare the extract containing the target peach nerve amine is also very special.

[Industrial availability]

Therefore, according to the present invention, a peach extract containing ceramide (sphingomyelin), glycosides, polyphenols, and active oxygen scavenging ability can be produced from peaches by a safe extraction method.

The peach extract obtained by the present invention can be used to extract various combinations of extraction solvents used to obtain peach extracts that can vary the content of the ingredients contained in the target, and can provide extracts that increase the content of peach neural amine, In addition, when an appropriate combination of purification methods such as activated carbon treatment and chromatography can be used to provide extracts that enhance the purification system, they can be used as food, cosmetics, or quasi-drugs, as well as processed materials.

And, in terms of raw materials, it is the sparse fruit peach produced in the production process of peach (Young peach), or peach processing residues produced in processing steps such as peach juice are also very special in terms of the effective use of by-products.

Claims (15)

A peach extract characterized by the following: a peach processing residue containing fibrous components obtained by removing seeds and fruit juice from sparse peach, ripe peach, or sparse peach or ripe peach, and passing through ethanol or an ethanol / hexane mixture Extraction, and then extracting the extract with hexane / water segment extraction to obtain active oxygen scavenging ability and sphingomyelin lipid (neuraminyl component). The peach extract as described in item 1 of the scope of the patent application, wherein the capacity of the hexane and water used for the extraction of sparse fruit peaches, ripe peaches, or peach processing residues, and the extraction of the extracts in stages The ratio is 1: 0.5: 0.5 to 1: 2: 2. The peach extract according to item 1 or 2 of the scope of the patent application, wherein the active oxygen scavenging energy is DPPH (diphenyl bitter hydrazine) activity. The peach extract according to any one of claims 1 to 3, wherein the DPPH activity is more than 5% and the neural amine content is more than 0.5%. A method for producing a peach extract, which is a method for producing a peach extract having active oxygen scavenging ability and containing a sphingomyelin lipid (neuraminylamine component), which is characterized in that the fruit peach, the ripe peach, or the fruit After the peaches or ripe peaches have been removed from the seeds and fruit juice, the fiber-containing peach processing residue is extracted with ethanol or an ethanol / hexane mixed solution, and the resulting extract is extracted with hexane / water in stages. The method for producing a peach extract as described in item 5 of the scope of patent application, wherein the extraction liquid used for the extraction of sparse peach, ripe peach, or peach processing residue, the hexane used for the staged extraction of the extraction liquid, and The capacity ratio of water is 1: 0.5: 0.5 to 1: 2: 2. A food or a processed product thereof comprising the peach extract according to any one of claims 1 to 4 of the scope of patent application. The food or the processed product thereof according to item 7 of the scope of patent application, wherein the peach extract as described in any of the scope of claims 1 to 4 and the cyclodextrin are mixed to make a powder. The food or the processed product thereof according to item 7 of the scope of patent application is in a state of being an aqueous solution containing the peach extract according to any one of the scope of patent applications 1 to 4. A cosmetic or a processed product thereof, comprising the peach extract according to any one of claims 1 to 4 of the scope of patent application. The cosmetic or processed product thereof according to item 10 of the scope of patent application, wherein the peach extract according to any one of claims 1 to 4 and the cyclodextrin are mixed to form a powder. The cosmetic product or processed product thereof according to item 10 of the scope of patent application is in a state of being an aqueous solution containing the peach extract according to any one of claims 1 to 4 of the scope of patent application. A quasi-drug or a processed product containing the peach extract according to any one of claims 1 to 4 of the scope of patent application. The quasi-drug or its processed product according to item 13 of the scope of patent application, wherein the peach extract as described in any one of the scope of patent applications 1 to 4 is mixed with cyclodextrin to form a powder . The quasi-drug or its processed product as described in item 13 of the scope of patent application is in a state of being an aqueous solution containing the peach extract as described in any one of the scope of patent applications 1 to 4.