JP6358418B2 - Method for producing Lamiaceae plant extract - Google Patents

Description

  The present invention relates to a method for producing a Labiatae plant extract.

  Many Lamiaceae plants are rich in aroma components and antioxidant components and have been used as so-called herbs such as spices and medicinal herbs since ancient times. Various studies have been conducted focusing on various physiological activities brought about by functional components of Lamiaceae plants. For example, an antiallergic agent (Patent Document 1) containing a hyaluronidase inhibitor extracted from water from a Labiatae plant selected from the group consisting of rosemary, thyme and Melissa as an active ingredient (Patent Document 1), Labiatae, Camphoraceae, Myrtaceae and In vivo antioxidant containing at least one selected from the group consisting of Asteraceae plants as an active ingredient (Patent Document 2), or a group consisting of Lamiaceae, Asteraceae, Gingeraceae, Rosaceae, and Apiaceae plants A platelet aggregation inhibitor (Patent Document 3) containing a fraction of an extract of at least one selected plant as an active ingredient is disclosed.

  Luteolin is a flavone contained in plants such as rosemary and horsetail, and has functionality such as anti-inflammatory action (Non-patent Document 1) and anti-cancer action (Non-Patent Document 2). For example, luteolin inhibits the growth of various types of tumor cells in in vitro tests (IC50: 3 to 50 μM), and also effectively inhibits tumor growth in in vivo tests (in foods) When 50 to 200 ppm of luteolin is blended, 0.1 to 0.3 mg / kg (body weight) of luteolin per day is administered intragastrically or 5 to 10 mg / kg (body weight) of luteolin is administered intraperitoneally) Has been reported (Non-Patent Document 3).

  Lamiaceae plants contain many functional components including luteolin in the form of glycosides. However, it has been reported that these functional components are more effective when administered in the form of aglycone than glycosides (Non-patent Document 4). Also, Lamiaceae plants contain components with irritating odors such as carvacrol and thymol, and have a unique strong odor, so there are restrictions on continuous intake of functional components contained in Lamiaceae plants. There is a problem of receiving. For the purpose of reducing such a unique strong odor, for example, a plant containing an antioxidant component is extracted with an aqueous alcohol solution having an alcohol concentration of 20 to 80% by weight, and the alcohol concentration of the obtained plant extract is 10% by weight. An antioxidant production method (Patent Document 4) is disclosed, which is characterized by passing the solution through a synthetic adsorbent after the content is reduced to not more than%.

  Thus, there is still a need for a safe functional material containing luteolin having functionality such as anti-inflammatory action and anti-cancer action.

JP-A-8-333267 JP 2011-236149 A JP 2012-153671 A Japanese Patent Laid-Open No. 2002-3840

Planta Medica, 2007, Vol. 73, No. 3, p. 221-226 Indian Journal of Clinical Biochemistry, 2012, Vol. 27, No. 2, p. 157-163 Molecules, 2008, Vol. 13, p. 2628-2651 The Journal of Pharmacology and Experimental Therapies, 2001, Vol. 296, No. 1, p. 181-187

  An object of the present invention is to provide a method for producing a Labiatae plant extract containing luteolin, which is a functional component, using a Labiatae plant as a raw material. Furthermore, the subject of this invention is providing the manufacturing method of the Labiatae plant extract which contains the functional ingredient luteolin and reduced the characteristic strong smell of the Labiatae plant.

As a result of intensive studies, the present inventors have used a Lamiaceae plant as a raw material, subjecting the raw material to an enzyme treatment using an enzyme having β-glucosidase activity, and after adding oils and fats and stirring treatment, It has been found that the above-mentioned problems can be solved by separating and removing oils and fats, and the present invention has been completed.
In addition, the Labiatae plant in this specification means the plant which belongs to Labiatae, or the plant of Labiatae.

  That is, the present invention is a method for producing a Lamiaceae plant extract containing luteolin, which is a functional component, obtained by subjecting a Lamiaceae plant as a raw material to enzymatic treatment of the raw material with an enzyme having β-glucosidase activity. A method is provided. The present invention further includes luteolin, which is a functional ingredient, obtained by separating and removing the fat after adding and stirring the fat and oil, and has a characteristic strong smell of Lamiaceae The present invention provides a method for producing a Lamiaceae plant extract with reduced content.

The present invention includes the following aspects.
Item (1)
A method for producing a Labiatae plant extract characterized by containing luteolin obtained from a Labiatae plant as a raw material and subjecting the raw material to an enzyme treatment using an enzyme having β-glucosidase activity.
Item (2)
The production method according to item (1), wherein the luteolin is free.
Item (3)
Item (1) or Item (2), wherein the Labiatae plant is at least one selected from the group consisting of thyme, sage, rosemary or marjoram.
Item (4)
The production method according to any one of Items (1) to (3), wherein the raw material is a pulverized product or an extract.
Item (5)
Furthermore, after adding fats and oils and stirring treatment, separating and removing the fats and oils reduces the characteristic strong odor of Lamiaceae plants, any one of the items (1) to (4) The production method according to claim 1.
Item (6)
The stirring treatment performed by adding fats and oils is performed in parallel with the enzyme treatment using an enzyme having β-glucosidase activity or after the enzyme treatment using an enzyme having β-glucosidase activity. Production method.
Item (7)
A Labiatae plant extract obtained by the production method according to any one of Items (1) to (6).
Item (8)
A beverage, food, quasi-drug or feed containing the Labiatae plant extract according to item (7).

According to the present invention, a Labiatae plant extract containing luteolin, which is a functional component, can be provided. Furthermore, according to the present invention, there is provided a Lamiaceae plant extract containing a functional ingredient luteolin and reducing the characteristic strong odor of Lamiaceae plants by components having a stimulating odor such as carvacrol. Can do.
By using the Labiatae plant extract according to the present invention, it is possible to provide a beverage, food, quasi-drug or feed utilizing the functionality of luteolin.

  In the present invention, the Lamiaceae plant as a raw material is generally used for food, and any plant belonging to the family Lamiaceae may be used. For example, thyme, sage, rosemary, marjoram, oregano, savory, basil, lemon balm, mint, sesame and perilla etc. are preferred, preferably thyme, sage, rosemary, marjoram, oregano, more preferably Thyme, sage or rosemary. The Labiatae plant used in the present invention can be used in a raw state or a dried one. Moreover, the part of the Labiatae plant used in the present invention is not particularly limited, and any of leaves, stems, roots, fruits, seeds, and the like may be used. The raw Lamiaceae plant may be used alone or in combination of two or more.

  In the present invention, the raw Lamiaceae plant may be a pulverized product or an extract, and among them, it is preferable to use a Lamiaceae plant extract.

  The pulverized material of the Lamiaceae plant may be a pulverized material obtained by pulverizing the Lamiaceae plant as a raw material alone or by adding a solvent such as water. The method for pulverizing the raw Lamiaceae plant is not particularly limited, and methods generally used for processing foods can be used alone or in combination. Examples of the equipment used for the pulverization include various cutting machines and pulverizers that process using cutting, pulverization, friction, air pressure, water pressure, and the like. As the pulverized product of the Lamiaceae plant, a liquid part obtained by pulverization as it is or after solid-liquid separation can be used. Furthermore, the pulverized product of Lamiaceae can be appropriately mixed with additives such as a pH adjuster.

  The extract of the Labiatae plant should just be the extract which extracted the Labiatae plant of a raw material with water, alcohol, or a water-alcohol mixed solution. The method for extracting the raw Lamiaceae plant is not particularly limited, and known means can be extracted alone or in combination. Examples of extraction methods include room temperature extraction, heat extraction, pressure extraction, stirring extraction, and ultrasonic extraction. The raw Lamiaceae plant may be extracted as it is, or may be used after being shredded or crushed. The extract of the Labiatae plant can use the liquid part obtained after extraction or by solid-liquid separation. Among these, a liquid part obtained by solid-liquid separation after extraction is preferable. Moreover, the extract of the Labiatae plant can use the concentrate. Furthermore, the extract of the Labiatae plant can mix | blend additives, such as a pH adjuster, suitably. Examples of the extract of Lamiaceae plants include thyme extract, sage extract, rosemary extract, marjoram extract, oregano extract, savory extract, basil extract, lemon balm extract, mint extract, egoma extract, perilla extract and the like.

  In the present invention, a Lamiaceae plant is used as a raw material, and the raw material is subjected to an enzyme treatment using an enzyme having β-glucosidase activity. The enzyme having β-glucosidase activity used in the present invention may be any enzyme having β-glucosidase activity that can be used in foods, and may include the enzyme. It can also be used in the form of an enzyme preparation. Examples of the enzyme having β-glucosidase activity include aromase (registered trademark) (manufactured by Amano Enzyme Co., Ltd.), Sumiteam (registered trademark) BGA (manufactured by Shin Nippon Chemical Industry Co., Ltd.), and rapidase (registered). Trademark) AR2000 (manufactured by DSM Japan Ltd.) and the like.

  In the present invention, the temperature for enzyme treatment using an enzyme having β-glucosidase activity is usually 20 to 70 ° C., preferably 30 to 65 ° C., more preferably 40 to 60 ° C. In the present invention, the enzyme treatment time using an enzyme having β-glucosidase activity is usually 30 minutes to 30 hours, preferably 1 to 24 hours, more preferably 2 to 20 hours. In the present invention, the amount of the enzyme having β-glucosidase activity can be appropriately changed depending on the treatment temperature and treatment time. 01 to 5% by weight, preferably 0.05 to 3% by weight, more preferably 0.1 to 2% by weight.

  In the present invention, before the enzyme treatment with an enzyme having β-glucosidase activity, the pH of the raw Lamiaceae plant is preferably adjusted to around the optimum pH of the enzyme having β-glucosidase activity. The pH to be adjusted is usually pH 2-8, preferably pH 3-6. When the pH is adjusted, neutralization may be performed after the enzyme treatment. For pH adjustment and neutralization treatment, those generally used in foods as pH adjusters can be used. The pH adjuster is not particularly limited as long as it is designated as a food additive. Examples of the pH adjuster include hydrochloric acid, citric acid, acetic acid, sodium hydroxide, sodium carbonate, sodium hydrogen carbonate and the like.

In the present invention, the content of luteolin, which is a functional component contained in the obtained Lamiaceae plant extract, may be at least contained, preferably 50 ppm or more per solid, more preferably 200 ppm per solid. As mentioned above, More preferably, it is 500 ppm or more per solid substance, Most preferably, it is 1000 ppm or more per solid substance.
In addition, the luteolin which the Labiatae plant extract contains in this invention means the thing of what is called a free state which exists in the state of an aglycon.

  In the present invention, the enzyme deactivation treatment is performed after the enzyme treatment. The enzyme deactivation treatment may be performed so that the enzyme activity does not remain in the finally obtained Labiatae plant extract, and the conditions for the enzyme deactivation treatment are the conditions under which the enzyme used in the enzyme treatment is deactivated. If there is no particular limitation.

In the present invention, the raw Lamiaceae plant may be agitated by adding fats and oils. In the present invention, an enzyme treatment using an enzyme having β-glucosidase activity and an oil / fat are added and stirred, and then the fat / oil is separated and removed to efficiently contain the functional ingredient luteolin. In addition, it is possible to produce a Labiatae plant extract that effectively reduces the characteristic strong odor of Labiatae plants due to components having an irritating odor such as carvacrol.
When the oil and fat is added and the stirring treatment is performed, the stirring treatment can be performed in parallel with the enzyme treatment using an enzyme having β-glucosidase activity or after the enzyme treatment using an enzyme having β-glucosidase activity.
Moreover, separation / removal of the oil and fat performed after the stirring treatment can be performed after the enzyme treatment using the enzyme having the β-glucosidase activity.

  The fats and oils used in the present invention may be any fats and oils that can generally be used in foods, and may contain the fats and oils. Examples of the fats and oils used in the present invention include rapeseed oil, soybean oil, corn oil, sunflower oil, safflower oil, rice bran oil, cottonseed oil, olive oil, sesame oil, peanut oil, grape seed oil, persimmon oil, perilla oil, linseed oil, walnut Oil, mustard oil, pumpkin seed oil, watermelon seed oil, coconut oil, almond oil, hazelnut oil, macadamia nut oil, cashew nut oil, pistachio oil, wheat germ oil, tea seed oil, avocado oil, papaya oil, palm oil, palm kernel Vegetable oils such as oil, coconut oil, cacao butter; animal oils such as lard, head, chicken oil, fish oil, horse oil, whale oil, seal oil, milk fat; and hydrogenated oils, fractionated oils, etc. .

  In the present invention, the method of adding oil and fat and stirring is not particularly limited, and known means generally used for stirring can be used. As a method of adding fats and oils and stirring, for example, a method of stirring a stirring object with a mechanical or magnetic stirrer using a stirring blade or a stirring bar, a stirring process by blowing a gas such as air or nitrogen into the stirring object And a method of stirring the stirring target by shaking, rotating, or the like a container containing the stirring target.

  In the present invention, the temperature at which the oil and fat is added and stirred may be any temperature as long as the oil or fat to be added has fluidity, but is usually 20 to 100 ° C, preferably 30 to 90 ° C, more preferably 35 to 80. ° C. Moreover, in this invention, the time which adds fats and oils and stir-processes is at least 5 minutes or more, and is normally 10 minutes-30 hours, Preferably it is 20 minutes-24 hours, More preferably, it is 30 minutes-20 hours. Further, in the present invention, the amount of oil and fat to be agitated can be appropriately changed depending on the treatment temperature and the treatment time, but is usually 0 by weight with respect to the solid matter in the treatment liquid to which the oil and fat is added. 2 to 20 times, preferably 0.5 to 15 times, more preferably 1 to 10 times.

  In the present invention, when oil and fat are added and stirring is performed, the used oil and fat is separated and removed. The method for separating and removing the oil and fat is not particularly limited, and can be performed by a known method such as centrifugation, decantation, use of an oil adsorbent or an oil flocculant.

  The Labiatae plant extract obtained by the present invention has good flavor and excellent palatability, so it can be used as it is, but it can also be used as a liquid part obtained by solid-liquid separation. it can. The method for solid-liquid separation is not particularly limited, and can be performed by a known method such as filtration or centrifugation. Further, the Labiatae plant extract obtained by the present invention may be used as a concentrate by treating the liquid part as it is or separated into solid and liquid with a concentrator or the like by a conventional method, or may be used after drying. The drying method is not particularly limited, and the drying can be performed using a known means. As a drying method, for example, a known means such as a spray dryer, a drum dryer, a freeze dryer, or an air dryer can be used. Further, an excipient such as dextrin may be added and dried. Further, the product obtained by drying may be pulverized and used as a powder or the like, and if necessary, it can be made into a granule using a granulator or the like.

  The Labiatae plant extract obtained by the present invention can be used as it is or diluted with water or the like. Furthermore, Labiatae plant extracts obtained by the present invention are various processed foods such as instant foods, dairy products, confectionery, seasonings, tea beverages, vegetable beverages, soy beverages, soy milk beverages and various beverages and foods. It can also be added and blended as appropriate. Moreover, it can also use together with what is used as a raw material or additive of a normal drink or food as needed.

  The Labiatae plant extract obtained by the present invention can be used for foods such as foods for specified health use, functional foods, and dietary supplements, quasi drugs, feeds, and the like. The form can be ampoules, capsules, pills, tablets, powders, granules, solids, liquids, gels, aerosols, etc., and can be blended in various products. In preparing these products, excipients, binders, lubricants and the like can be appropriately blended.

  EXAMPLES Hereinafter, although an Example is shown and this invention is demonstrated concretely, this invention is not limited by the following examples. In this example, the blending ratio, content ratio, and concentration of each raw material and material are all based on parts by weight unless otherwise specified.

[Preparation 1]
By adding 760 g of water to 40 g of thyme powder (manufactured by KIS Co., Ltd.), extracting at 90 ° C. for 30 minutes, and then performing solid-liquid separation by filtering using a filter cloth (nonwoven fabric), 640 g of extract (solid content: 1.64%) (Preparation 1) was obtained. About the obtained time extract, as a result of measuring the content of luteolin, which is a functional component, and carvacrol, which is a stimulating odor component, under high-performance liquid chromatography (hereinafter referred to as “HPLC”) under the following measurement conditions, The content per solid was luteolin: not detected, carvacrol: 1053 ppm.

<HPLC measurement conditions (Luteolin)>
Detector: UV detector (ultraviolet wavelength 254 nm)
Column: Inert Sustain C18 (inner diameter 4.6 mm, length 250 mm)
Mobile phase A: 15% by volume acetonitrile aqueous solution (containing 0.1% by volume acetic acid)
Mobile phase B: 35% by volume acetonitrile aqueous solution (containing 0.1% by volume acetic acid)
Gradient: linear gradient from mobile phase A to mobile phase B (50 minutes)
Flow rate: 1.5 ml / min Column temperature: 40 ° C
Standard: Luteolin (manufactured by Tokyo Chemical Industry Co., Ltd.) was dissolved in 80% acetonitrile aqueous solution to prepare a calibration curve.
Specimen: A sample appropriately diluted with 80% acetonitrile aqueous solution.

<HPLC measurement conditions (carbachlor)>
Detector: UV detector (ultraviolet wavelength 280 nm)
Column: Inert Sustain C18 (inner diameter 4.6 mm, length 250 mm)
Mobile phase A: 20% by volume acetonitrile aqueous solution Mobile phase B: Acetonitrile gradient: Linear gradient from mobile phase A to mobile phase B (40 minutes)
Flow rate: 1.0 ml / min Column temperature: 40 ° C
Standard: Carbacrol (manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved in 80% acetonitrile aqueous solution to prepare a calibration curve.
Specimen: A sample appropriately diluted with 80% acetonitrile aqueous solution.

[Example 1]
160 g of the time extract obtained in Preparation 1 is put in an Erlenmeyer flask, 0.32 g of aromase which is a β-glucosidase preparation is added, and the enzyme treatment is performed at 60 ° C. for 3 hours, followed by enzyme deactivation treatment at 80 ° C. for 30 minutes. went. Subsequently, 140 g (solid content 1.80%) (Example 1) of the Labiatae plant extract of this invention was obtained by carrying out centrifugation (2500xG, 5 minutes) and carrying out solid-liquid separation. About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and the carvacrol content by HPLC in the same manner as in Preparation 1, the contents per solid were: luteolin: 10800 ppm, carvacrol: 885 ppm Met.

[Preparation 2]
By adding 540 g of water to 30 g of sage powder (manufactured by KIS Co., Ltd.), extracting at 90 ° C. for 30 minutes, and performing solid-liquid separation by filtering using a filter cloth (nonwoven fabric), sage 450 g of extract (solid content: 1.43%) (Preparation 2) was obtained. About the obtained sage extract, as a result of measuring the luteolin content and carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was luteolin: not detected, carvacrol: 51 ppm.

[Example 2]
160 g of the sage extract obtained in Preparation 2 is put in an Erlenmeyer flask, 0.32 g of aromase which is a β-glucosidase preparation is added, the enzyme treatment is performed at 60 ° C. for 3 hours, and then the enzyme inactivation treatment is performed at 80 ° C. for 30 minutes. went. Subsequently, 140 g (solid content 1.81%) (Example 2) of the Labiatae plant extract of this invention was obtained by carrying out centrifugation (2500xG, 5 minutes) and carrying out solid-liquid separation. About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was luteolin: 5976 ppm, carvacrol: 34 ppm Met.

[Preparation 3]
By adding 380 g of water to 20 g of rosemary powder (manufactured by KIS Co., Ltd.), extracting at 90 ° C. for 30 minutes, and then performing solid-liquid separation by filtering using a filter cloth (nonwoven fabric), 300 g of rosemary extract (solid content 1.35%) (Preparation 3) was obtained. About the obtained rosemary extract, as a result of measuring the luteolin content and carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was luteolin: not detected, carvacrol: 65 ppm. .

[Example 3]
160 g of rosemary extract obtained in Preparation 3 is placed in an Erlenmeyer flask, 0.32 g of aromase, which is a β-glucosidase preparation, is added, and after 3 hours of enzyme treatment at 60 ° C., the enzyme is inactivated at 80 ° C. for 30 minutes. Went. Subsequently, 140 g (solid content 1.55%) (Example 3) of the Labiatae plant extract of this invention was obtained by carrying out solid-liquid separation by centrifugation (2500xG, 5 minutes). About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was luteolin: 5255 ppm, carvacrol: 43 ppm Met.

[Example 4]
190 g of water was added to 10 g of thyme powder (manufactured by KIS Co., Ltd.), 20 g of soybean oil (manufactured by Kato Oil Co., Ltd.) was added, and the mixture was extracted at 90 ° C. for 30 minutes with stirring. Solid-liquid separation was performed using a cloth (nonwoven fabric). 150 g of the obtained filtrate was put into an Erlenmeyer flask, 0.3 g of aromase, which is a β-glucosidase preparation, was added, and the enzyme treatment was performed at 60 ° C. for 3 hours, followed by enzyme deactivation treatment at 80 ° C. for 30 minutes. Subsequently, the oil phase was separated and removed by centrifugation (2500 × G, 5 minutes) to obtain 140 g of the Labiatae plant extract of the present invention (solid content: 1.93%) (Example 4). About the obtained Labiatae plant extract of the present invention, as a result of measuring the luteolin content and carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was as follows: luteolin: 10600 ppm, carvacrol: 130 ppm Met.

[Example 5]
160 g of the time extract obtained in Preparation 1 is put in an Erlenmeyer flask, 0.32 g of aromase which is a β-glucosidase preparation is added, and 16 g of soybean oil (manufactured by Kato Oil Co., Ltd.) is added and stirred. After enzyme treatment at 60 ° C. for 3 hours, enzyme inactivation treatment was performed at 80 ° C. for 30 minutes. Subsequently, the oil phase was separated and removed by decantation to obtain 140 g of a Labiatae plant extract of the present invention (solid content 1.91%) (Example 5). About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and the carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was as follows: luteolin: 10100 ppm, carvacrol: not It was a detection.

[Example 6]
160 g of the time extract obtained in Preparation 1 is put in an Erlenmeyer flask, 0.32 g of aromase which is a β-glucosidase preparation is added, and the enzyme treatment is performed at 60 ° C. for 3 hours, followed by enzyme deactivation treatment at 80 ° C. for 30 minutes. went. Next, 16 g of soybean oil (manufactured by Kato Oil Co., Ltd.) was added, and after stirring at 60 ° C. for 30 minutes, the oil phase was separated and removed by centrifugation (2500 × G, 5 minutes). 140 g of the Labiatae plant extract of the present invention (solid content 1.94%) (Example 6) was obtained. About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and the carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was as follows: luteolin: 10700 ppm, carvacrol: not It was a detection.

[Sensory evaluation 1]
About the Labiatae plant extract of this invention obtained in Example 4, Example 5, Example 6, and Example 1, the sensory evaluation by 10 panelists was implemented by using as a sample each diluted 5-fold with tap water. . The evaluation was performed by comparing each sample with the score of the thyme extract of Preparation 1 diluted in the same manner as the sample for the characteristic odor of Lamiaceae plants, and the average value was calculated. The results are shown in Table 1.

As shown in Table 1, Example 4, Example 5 and Example 6 Labiatae plant extracts added with fats and oils were stirred, and the characteristic odor of Labiatae plants was enzyme-treated. Compared with the time extract of Preparation 1 which was not performed, it was sufficiently reduced and palatability was improved. On the other hand, when the agitation treatment with addition of fats and oils was not performed (Example 1), it had almost the same odor and flavor as the thyme extract of Preparation 1 not subjected to the enzyme treatment.
Moreover, about the content of carvacrol which is an irritating odor component, the Labiatae plant extracts of Examples 4, 5 and 6 of the present invention in which fats and oils were added and subjected to stirring treatment were not detected or Compared to Preparation 1, the content was low, and Example 1 had a high content approximating that of Preparation 1.

[Example 7]
160 g of the sage extract obtained in Preparation 2 was put in an Erlenmeyer flask, 0.16 g of aromase which is a β-glucosidase preparation was added, and 32 g of lard (manufactured by Ueda Oil Co., Ltd.) was further added, while stirring, After enzyme treatment at 5 ° C. for 5 hours, enzyme inactivation treatment was performed at 90 ° C. for 10 minutes. Subsequently, the oil phase was separated and removed by centrifugation (2500 × G, 5 minutes) to obtain 140 g of the Labiatae plant extract of the present invention (solid content: 1.83%) (Example 7). About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was luteolin: 4946 ppm, carvacrol: not It was a detection.

[Sensory evaluation 2]
About the Labiatae plant extract of this invention obtained in Example 7 and Example 2, the sensory evaluation by 10 panelists was implemented by using as a sample each diluted 5-fold with tap water. The evaluation was performed by comparing each sample with the score of the sage extract of Preparation 2 diluted in the same manner as the sample for the characteristic odor of Lamiaceae plants, and the average value was calculated. The results are shown in Table 2.

As shown in Table 2, the Lamiaceae plant extract of Example 7 of the present invention, which was added with fats and oils and stirred, was a sage extract of Preparation 2 in which the characteristic odor of Lamiaceae plants was not subjected to enzyme treatment Compared with, it was sufficiently reduced and palatability was improved. On the other hand, when the stirring process which added fats and oils was not performed (Example 2), it had the smell and flavor substantially equivalent to the sage extract of the preparation 2 which has not performed the enzyme process.
In addition, with respect to the content of carvacrol which is an irritating odor component, the Labiatae plant extract of the present invention of Example 7 in which fats and oils were added and subjected to a stirring treatment, no carvacrol was detected, and Example 2 was prepared 2 The content was approximately.

[Example 8]
150 g of rosemary extract obtained in Preparation 3 is put in an Erlenmeyer flask, 0.3 g of aromase which is a β-glucosidase preparation is added, and 7.5 g of rapeseed oil (manufactured by Kato Oil Co., Ltd.) is further added and stirred. However, after enzyme treatment at 60 ° C. for 5 hours, enzyme inactivation treatment was performed at 90 ° C. for 10 minutes. Subsequently, 130 g (solid content 1.66%) (Example 8) of the Labiatae plant extract of this invention was obtained by isolate | separating and removing an oil phase by centrifugation (2500xG, 10 minutes). About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and the carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was as follows: luteolin: 4665 ppm, carvacrol: not It was a detection.

[Sensory evaluation 3]
About the Labiatae plant extract of this invention obtained in Example 8 and Example 3, the sensory evaluation by 10 panelists was implemented by using as a sample each diluted 5 times with tap water. Evaluation was scored by comparing each sample with the score of the rosemary extract of Preparation 3 diluted in the same manner as the sample for the characteristic odor of Lamiaceae plants, and the average value was calculated. The results are shown in Table 3.

As shown in Table 3, the Lamiaceae plant extract of Example 8 of the present invention, which was added with fats and oils and stirred, was characterized by the characteristic odor of Lamiaceae plants, but rosemary of Preparation 3 that was not subjected to enzyme treatment. Compared with the extract, it was sufficiently reduced and the palatability was improved. On the other hand, in the case where the stirring treatment with the addition of fats and oils was not carried out (Example 3), it had almost the same odor and flavor as those of the rosemary extract of Preparation 3 which was not subjected to the enzyme treatment.
In addition, with respect to the content of carvacrol, which is an irritating odor component, carbachlor was not detected in the Labiatae plant extract of Example 8 of the present invention, which was added with fats and oils and stirred, and Example 3 was prepared 3 The content was approximately.

[Example 9]
180 g of water was added to 20 g of marjoram powder (manufactured by KIS Co., Ltd.), extracted at 90 ° C. for 30 minutes, and then filtered using a filter cloth (nonwoven fabric) to perform solid-liquid separation. 100 g of the obtained filtrate was put into an Erlenmeyer flask, 1.0 g of aromase as a β-glucosidase preparation was added, and the enzyme treatment was performed at 50 ° C. for 20 hours, followed by enzyme deactivation treatment at 90 ° C. for 30 minutes. Next, 15 g of palm oil (produced by Fuji Oil Co., Ltd.) is added, and after stirring at 80 ° C. for 30 minutes, the oil phase is separated and removed by centrifugation (2500 × G, 5 minutes). Thus, 90 g of the Labiatae plant extract of the present invention (solid content: 4.95%) (Example 9) was obtained. About the obtained Labiatae plant extract of this invention, as a result of measuring the luteolin content and the carvacrol content by HPLC in the same manner as in Preparation 1, the content per solid was luteolin: 6121 ppm, carvacrol: not It was a detection.

Claims (4)

  By using a Labiatae plant as a raw material, the raw material is enzymatically treated with an enzyme having β-glucosidase activity, and after adding and stirring the oil and fat, the fat and oil is separated and removed, whereby the characteristics of the Labiatae plant A method for producing a luteolin-containing Labiatae plant extract, characterized by reducing a strong strong odor.   The production according to claim 1, wherein the stirring treatment performed by adding fats and oils is performed in parallel with the enzyme treatment using an enzyme having β-glucosidase activity or after the enzyme treatment using an enzyme having β-glucosidase activity. Method. A luteolin-containing Lamiaceae plant extract obtained by the production method according to claim 1 or 2, wherein the characteristic strong odor of Lamiaceae is reduced .   A beverage, food, quasi-drug or feed containing the Labiatae plant extract according to claim 3.