TWI650556B - Blood glucose test piece and preparation method thereof - Google Patents

Abstract

The invention relates to a blood glucose test strip, which includes a substrate and a test circuit formed on a surface of the substrate at intervals. Each test circuit includes a support layer, an adhesion layer, and a metal layer. The support layer is formed on the substrate. The adhesion layer is formed on a support layer, and the metal layer is formed on the adhesion layer. The invention also provides a method for making the blood glucose test strip.

Description

Blood glucose test strip and manufacturing method thereof

The invention relates to a blood glucose test piece and a manufacturing method thereof.

In the 20th century, with the advancement of the medical system, the average life expectancy of humans has continued to increase, but various civilized diseases have also increased. Diabetes is one of the important examples. According to the WHO report, about 810,000 people died of diabetes worldwide in 2000, and about 14.95 million people had diabetes. In most developed countries, diabetes is the fourth most common cause of death. It is expected that by 2025, the number of diabetic patients worldwide will increase to 300 million, accounting for 1.67% of the global population. This huge number makes us have to pay attention to diabetes. Monitoring blood glucose levels over time is an important step in controlling diabetes. At present, the commonly used method for detecting and monitoring blood glucose levels is mainly to use blood glucose test strips to test blood. Blood glucose test strips are an advanced glucose biosensor. The blood glucose test strip has a number of conductive strips, each of which has electrodes and detection terminals at both ends, and the electrodes are covered with a reaction layer with glucose oxidase; when blood penetrates into the reaction layer, an electrochemical reaction occurs. An electrical signal is generated, which is transmitted to the detection terminal through the conductive strip, and then the blood glucose test strip is inserted into the reading slot of the blood glucose meter to determine the blood glucose content in the blood.

As a blood glucose testing device that is more commonly used at present, the blood glucose test strip has a large market demand. The manufacturing method of the conventional blood glucose test strip is usually to form multiple Conductive strips of blood glucose test strips, and then cut the motherboard into multiple blood glucose test strips. However, the current cost of blood glucose test strip materials is relatively high.

Aiming at the above-mentioned shortcomings of blood glucose test strips in the conventional technology, the present invention provides a new blood glucose test strip and a manufacturing method thereof, which can increase the output of blood glucose test strips per unit area of the main board, thereby achieving the improvement of material utilization rate and cost reduction. purpose.

A blood glucose test strip includes a substrate and test lines formed on one surface of the substrate. Each test line includes a support layer, an adhesion layer, and a metal layer. The support layer is formed on the substrate, and the adhesion layer is formed. On the support layer, the metal layer is formed on the adhesion layer.

A method for manufacturing a blood glucose test strip includes the steps of: providing a substrate, forming a support layer on one surface of the substrate, the support layer being formed on the substrate at intervals, and patterningly covering a part of the surface of the substrate; An adhesion layer is formed on the surface of the substrate covered by the support layer; a metal layer is formed on the surface of the adhesion layer; a portion of the adhesion layer and the metal layer other than the support layer is removed to obtain a test circuit on the substrate.

Compared with the conventional technology, the blood glucose test strip of the present invention adds a support layer between the adhesion layer and the substrate. Due to the height difference between the support layer and the substrate, a groove is formed between the support layers of adjacent test circuits. The presence of the grooves can increase the area of the metal layer, that is, the enzyme reaction area per unit length of the test line, and thereby increase the output of blood glucose test strips per unit area of the motherboard, thereby achieving the purpose of increasing material utilization and reducing costs.

10, 20, 30, 40, 50‧‧‧ blood glucose test strips

210, 310, 410, 510‧‧‧ substrate

200, 300, 400, 500‧‧‧ test lines

220, 320, 420, 520‧‧‧ support layer

221, 221‧‧‧ side

521‧‧‧Main body

523‧‧‧ raised

230, 330, 430, 530‧‧‧ Adhesive layers

240, 340, 440, 540‧‧‧ metal layers

250, 350, 450, 550‧‧‧ groove

FIG. 1 is a schematic perspective view of a first embodiment of a blood glucose test strip according to the present invention.

FIG. 2 is a schematic cross-sectional view taken along II-II in FIG. 1.

3 is a schematic cross-sectional view of a second embodiment of a blood glucose test strip according to the present invention.

4 is a schematic cross-sectional view of a third embodiment of a blood glucose test strip according to the present invention.

5 is a schematic top view of a fourth embodiment of a blood glucose test strip according to the present invention.

FIG. 6 is a schematic cross-sectional view taken along VI-VI in FIG. 5.

FIG. 7 is a schematic cross-sectional view of FIG. 5 along VII-VII.

8 is a schematic flowchart of a method for preparing a blood glucose test strip according to a preferred embodiment of the present invention.

In order to make the technical content disclosed in this application more detailed and complete, reference may be made to the drawings and the following specific embodiments of the present invention. The same reference numerals in the drawings represent the same or similar components. However, those skilled in the art should understand that the embodiments provided below are not intended to limit the scope covered by the present invention. In addition, the drawings are for illustrative purposes only, and are not drawn to their original dimensions.

The blood glucose test strip of the present invention includes a substrate and mutually spaced test circuits formed on the substrate. The test circuit includes an adhesion layer and a metal layer covering the adhesion layer. A support layer is added between the adhesion layer and the substrate. Due to the height difference between the support layer and the substrate, a trench is formed between adjacent test lines. The presence of the grooves can increase the enzyme reaction area per unit length of the test circuit, which in turn can increase the output of blood glucose test strips per unit area of the motherboard.

Hereinafter, specific embodiments of the present invention will be described in further detail with reference to the accompanying drawings.

As shown in FIGS. 1 and 2, the blood glucose test strip 20 according to the first embodiment of the present invention includes a base 210 and test lines 200 formed on the base 210 at intervals. Each test line 200 includes a support layer 220 and an adhesion layer 230. And a metal layer 240.

The substrate 210 is substantially plate-shaped, and may be made of a plastic material, such as a polyethylene glycol terephthalate (PET) material.

The test circuit 200 may include a detection terminal and an electrode connected to the detection terminal, wherein the detection terminal is used to react with an enzyme. The support layer 220 covers a part of the surface of the substrate 210 in a patterned manner. The material of the support layer 220 may be a resin composition, and specifically may be a photoresist, ink, or the like. In this embodiment, the support layer 220 is formed of a photoresist material.

The cross-sectional pattern of each supporting layer 220 along the II-II direction in FIG. 1 (that is, perpendicular to the surface of the substrate 210 and the extending direction of the supporting layer 220 at the same time) is generally a trapezoidal shape. The included angle θ of the surface is 30 ° or more and 90 ° or less.

The adhesion layer 230 is formed on the support layer 220 and completely covers the support layer 220, and the metal layer 240 is formed on the adhesion layer 230 and completely covers the adhesion layer 230. The material of the adhesion layer 230 is a metal with good electrical conductivity and good adhesion on the resin, and specifically may be copper (Cu) or silver (Ag), and the adhesion layer 230 may also be carbon. The material of the metal layer 240 may be a metal with relatively stable chemical properties, and specifically may be gold or palladium (Au, Pd). The adhesion layer 230 provides a better adhesion surface for the metal layer 240; the surface of the metal layer 240 is an enzyme reaction area, and its material has a good electrical conductivity, which can effectively conduct the test results. At the same time, the metal layer 240 is not easy to react with the enzyme to test results Make an impact.

Due to the height difference between the support layer 220 and the substrate 210, trenches 250 are formed between the support layers 220 of adjacent test lines 200. The number of the trenches 250 is at least two, and the narrowest width of the trenches 250 is greater than 50 μm.

The blood glucose test strip 20 includes a support layer 220 having a substantially trapezoidal cross-section between the adhesion layer 230 and the base 210. Due to the height difference between the support layer 220 and the base 210, a groove 250 is formed between adjacent test lines 200. The presence of the groove 250 can increase the area of the metal layer 240, that is, the enzyme reaction area per unit length test circuit, and thus the output of blood glucose test strips per unit area of the motherboard, thereby achieving the purpose of improving material utilization and reducing costs. .

As shown in FIG. 3, the blood glucose test strip 30 according to the second embodiment of the present invention includes a substrate 310 and test circuits 300 formed on the substrate 310 at intervals. Each test circuit 300 includes a support layer 320, an adhesion layer 330, and a metal. Layer 340.

The substrate 310 is substantially plate-shaped, and may be a plastic material, such as a polyethylene glycol terephthalate (PET) material.

The test circuit 300 may include a detection terminal and an electrode connected to the detection terminal, wherein the detection terminal is used to react with an enzyme. The support layer 320 covers a part of the surface of the substrate 310 in a patterned manner. The material of the support layer 320 may be a resin composition, and may specifically be a photoresist, ink, or the like. In this embodiment, the support layer 320 is formed of a photoresist material.

The cross-sectional pattern of each support layer 320 along the direction perpendicular to the surface of the substrate 310 and the extension direction of the support layer 320 is generally arcuate. The intersection point between the support layer 320 and the surface of the substrate 310 is tangent. The angle θ between the tangent and the surface of the substrate 310 is greater than or equal to 30 °. 90 ° or less.

The adhesion layer 330 is formed on the support layer 320 and completely covers the support layer 320, and the metal layer 340 is formed on the adhesion layer 330 and completely covers the adhesion layer 330. The material of the adhesion layer 330 is a metal with good conductivity and good adhesion on the resin. Specifically, the adhesion layer 330 may be copper (Cu) or silver (Ag), and the adhesion layer 330 may also be carbon. The material of the metal layer 340 may be a metal with relatively stable chemical properties, and specifically may be gold or palladium (Au, Pd). The adhesion layer 330 provides a better adhesion surface for the metal layer 340; the surface of the metal layer 340 is an enzyme reaction area, and its material has a good electrical conductivity, which can effectively conduct the test results. At the same time, the metal layer 340 does not easily react with the enzyme to test the results. Make an impact.

Due to the height difference between the support layer 320 and the substrate 310, trenches 350 are formed between the support layers 320 of adjacent test lines 300. The number of the trenches 350 is at least two, and the width of the narrowest part of the trenches 350 is greater than 50 μm.

The blood glucose test strip 30 includes a support layer 320 having an approximately arcuate cross-section between the adhesion layer 330 and the base 310. Since the support layer 320 has a height difference from the base 310, adjacent test lines A trench 350 is formed between 300. The presence of the grooves 350 can increase the area of the metal layer 340, that is, the enzyme reaction area per unit length test circuit can be increased, and the blood glucose test strip output per unit area of the motherboard can be increased, thereby achieving the purpose of improving material utilization and reducing costs. .

As shown in FIG. 4, the blood glucose test strip 40 according to the third embodiment of the present invention includes a substrate 410 and test circuits 400 formed on the substrate 410 at intervals. Each test circuit 400 includes a support layer 420, an adhesion layer 430, and a metal. Layer 440.

The substrate 410 is substantially plate-shaped, and may be made of a plastic material, such as a polyethylene glycol terephthalate (PET) material.

The test circuit 400 may include a detection terminal and an electrode connected to the detection terminal, wherein the detection terminal is used to react with an enzyme. The support layer 420 covers a part of the surface of the substrate 410 in a patterned manner. The material of the support layer 420 may be a resin composition, and specifically may be a photoresist, ink, or the like. In this embodiment, the supporting layer 420 is formed of a photoresist material.

A cross-sectional pattern of each support layer 420 along a direction perpendicular to the surface of the substrate 410 and the extending direction of the support layer 420 is an irregular polygon.

The adhesion layer 430 is formed on the support layer 420 and completely covers the support layer 420, and the metal layer 440 is formed on the adhesion layer 430 and completely covers the metal layer 440. The material of the adhesion layer 430 may be a metal with good conductivity and good adhesion on the resin, and specifically may be copper (Cu) or silver (Ag), and the adhesion layer 430 may also be carbon. The material of the metal layer 440 may be a metal with relatively stable chemical properties, and specifically may be gold or palladium (Au, Pd). The adhesion layer 430 provides a better adhesion surface for the metal layer 440; the surface of the metal layer 440 is an enzyme reaction area, and its material has a good electrical conductivity, which can effectively conduct the test results. At the same time, the metal layer 440 is not easy to react with the enzyme and test the results. Make an impact.

Due to the height difference between the support layer 420 and the substrate 410, trenches 450 are formed between the support layers 420 of adjacent test lines 400. The number of the trenches 450 is at least two, and the width of the narrowest part of the trenches 450 is greater than 50 μm.

The blood glucose test strip 40 includes a support layer 420 having an irregular polygonal cross section between the adhesion layer 430 and the substrate 410. Due to the height difference between the support layer 420 and the substrate 410, a groove 450 is formed between adjacent test lines 400. The presence of the groove 450 can increase the area of the metal layer 440, that is, the enzyme reaction area per unit length of the test circuit can be increased, thereby increasing the output of blood glucose test strips per unit area of the motherboard, thereby achieving the purpose of improving material utilization and reducing costs .

Please refer to FIG. 5, FIG. 6 and FIG. 7 together. The blood glucose test strip 50 according to the fourth embodiment of the present invention includes a substrate 510 and a test circuit 500 formed on the substrate 510. Each test circuit 500 includes a support layer 520, a The adhesion layer 530 and a metal layer 540.

The substrate 510 is substantially plate-shaped, and may be made of a plastic material, such as a polyethylene glycol terephthalate (PET) material. The support layer 520 covers a part of the surface of the substrate 510 in a patterned manner. The material of the support layer 520 may be a resin composition, and specifically may be a photoresist, ink, etc. In this embodiment, the support layer 520 is formed of a photoresist material. Each support layer 520 includes a main body portion 521. As shown in FIG. 6, the cross-sectional pattern of each support layer 520 along the VI-VI direction in FIG. 5 is substantially a rectangle. As shown in FIG. 7, each body portion 521 protrudes to form a convex portion 523 at both sides of the body portion 521 at a fixed distance. The cross-sectional pattern of the convex portion 523 along the VII-VII direction in FIG. 5 is substantially a triangle. In other embodiments, the cross-section of the protruding portion 523 may be other shapes, such as a fan shape. The adhesion layer 530 is formed on the support layer 520 and completely covers the support layer 520, and the metal layer 540 is formed on the adhesion layer 530 and completely covers the metal layer 540. The material of the adhesion layer 530 may be a metal with good conductivity and good adhesion on the resin, and specifically may be copper (Cu) or silver (Ag), and the adhesion layer 530 may also be carbon. The material of the metal layer 540 may be a metal with relatively stable chemical properties, and specifically may be gold or palladium (Au, pd). The adhesion layer 530 provides a better adhesion surface for the metal layer 540; the surface of the metal layer 540 is an enzyme reaction area, and its material has a good electrical conductivity, which can effectively conduct the test results. At the same time, the metal layer 540 is not easy to react with the enzyme to test the results Make an impact.

Due to the height difference between the support layer 520 and the substrate 510, grooves 550 are formed between the support layers 520 of adjacent test lines 500. The number of the grooves 550 is at least two, and the narrowest width of the grooves 550 is greater than 50 μm.

The blood glucose test strip 50 includes a support layer 520 between the adhesion layer 530 and the substrate 510. Due to the height difference between the support layer 420 and the substrate 410, a groove 450 is formed between adjacent test lines 500. At the same time, a plurality of convex portions 523 protrude from both sides of the rectangular body portion 521 of the support layer 50. The presence of the groove 450 can increase the area of the metal layer 440, and the raised portion 513 further increases the area of the metal layer 440, that is, the enzyme reaction area per unit length test line can be increased, and the blood glucose per unit area of the motherboard can be increased The output of test strips can achieve the purpose of increasing material utilization and reducing costs.

Referring to FIG. 8, a method for preparing a blood glucose test strip according to a preferred embodiment of the present invention includes the following steps:

In step S81, a substrate is provided, and a support layer is formed on one surface of the substrate. The support layer is formed on the substrate at intervals, and partially covers the surface of the substrate in a patterned manner. The material of the substrate may be a plastic material, such as a PET material. The material of the support layer may be a resin composition, and specifically may be a photoresist, ink, or the like. In this embodiment, the support layer is formed of a photoresist material. The method for forming the supporting layer may be: firstly coating or printing a resin composition on the surface of the motherboard, and then patterning the resin composition layer to obtain a supporting layer. Specifically, the method of patterning the resin composition layer may be a conventional exposure development technique. The method for forming the support layer may also be to directly form the patterned support layer covering the substrate by means of physical vapor deposition or chemical vapor deposition through a mask. The specific shape of the supporting layer may be, but is not limited to, the shapes in the first to fourth embodiments.

In step S82, an adhesion layer is formed on the surface of the support layer and the surface of the substrate not covered by the support layer. The material of the adhesion layer is a metal having good conductivity such as copper and silver and having good adhesion on the resin, and may also be carbon. The method for forming the adhesion layer may be physical vapor deposition or chemical vapor deposition, or may be formed by screen printing of silver paste or carbon paste.

In step S83, a metal layer is formed on the surface of the adhesion layer. The material of the metal layer may be a metal with relatively stable chemical properties, and specifically may be gold or palladium (Au, Pd). The method of forming the metal layer may be physical vapor deposition or chemical vapor deposition.

In step S84, a portion of the adhesion layer and the metal layer other than the support layer is removed by laser etching or exposure development to obtain a test circuit on the substrate, thereby obtaining a mother slice of the blood glucose test strip.

In step S85, the mother film is appropriately cut to obtain a plurality of blood glucose test pieces.

The shape of the cross-section pattern of the support layer of the blood glucose test strip of the present invention is not limited to the shapes listed in the above embodiments, and may also be other reasonable polygonal structures.

Hereinabove, specific embodiments of the present invention have been described with reference to the drawings. However, those of ordinary skill in the art can understand that various changes and substitutions can be made to the specific embodiments of the present invention without departing from the spirit and scope of the present invention. These changes and substitutions fall within the scope defined by the claims of the present invention.

Claims (9)

A blood glucose test strip includes a substrate and a test line formed on a surface of the substrate. The improvement is that each test line includes a support layer, an adhesion layer, and a metal layer, and the support layer is formed on the substrate. On the substrate, the adhesion layer is formed on the support layer, the metal layer is formed on the adhesion layer, and grooves are formed between adjacent support layers, and the number of the grooves is at least 2 The width of the narrowest part of the trench is greater than 50 μm. The blood glucose test strip according to claim 1, wherein a material of the support layer is a resin composition. The blood glucose test strip according to claim 2, wherein a material of the support layer is a photoresist material. The blood glucose test strip according to claim 1, wherein a cross-sectional pattern of the support layer along a direction perpendicular to the base surface and the extension direction of the support layer is trapezoidal, arcuate, or irregular polygon. The blood glucose test strip according to claim 4, wherein when the cross-sectional shape of the support layer is trapezoidal or arcuate, an angle θ angle formed between a side surface in which the support layer directly contacts the substrate and a surface of the substrate The range is 30 ° or more and 90 ° or less. The blood glucose test strip according to claim 1, wherein the substrate is made of plastic. The blood glucose test strip according to claim 1, wherein the adhesion layer is made of metal or carbon. The blood glucose test strip according to claim 1, wherein each support layer includes a main body portion and a convex portion formed on the main body portion and protruding from both sides at regular intervals. A method for manufacturing a blood glucose test strip includes the following steps: providing a substrate, forming a supporting layer on one surface of the substrate, the supporting layer being formed on the substrate at intervals, and covering a part of the surface of the substrate patternally; and on the supporting layer The surface of the substrate and the surface of the substrate not covered by the support layer form an adhesion layer; a metal layer is formed on the surface of the adhesion layer; a portion of the adhesion layer and the metal layer except for covering the support layer is removed to A test circuit is obtained on the substrate; wherein grooves are formed between adjacent support layers, the number of the grooves is at least 2, and the width of the narrowest part of the grooves is greater than 50 μm.