TWI605820B - An herbal composition for improving alcohol intoxication and a use of the herbal composition thereof - Google Patents

Description

Herbal compound composition for improving alcoholism and use thereof

The invention relates to a Chinese herbal compound composition, in particular to a Chinese herbal compound composition for improving alcoholism, and the invention relates to the use of the Chinese herbal compound composition for preparing an alcohol poisoning medicament.

Alcohol intoxication, also known as drunkenness, refers to the physiological state caused by alcohol (ethanol) intake. For example, low-dose ethanol intake can lead to more happiness, skin redness and misbehavior. State, high doses of ethanol intake may lead to loss of balance, muscle disorders, impaired judgment, etc., and may even cause coma or death.

Antrodia cinnamomea is a unique and precious medicinal material in Taiwan. It is grown only on the inner wall of hollow core material of Cinnamomum kanehirai , which is rich in triterpenoids. Triterpenoids have inhibitory cancer cells. The functions of growing, repairing the liver and promoting liver function, lowering blood lipids and blood pressure, and improving immunity can also help to alleviate the physiological state caused by alcoholism; however, the price of Antrodia camphorata is not suitable as an active ingredient for improving alcoholism. Development, and therefore still need to develop a herbal compound composition that can improve alcoholism to improve the aforementioned problems.

The present invention provides a Chinese herbal compound composition which can reduce the cost of improving the active ingredient of alcoholism.

The invention further provides a use of a Chinese herbal compound composition as an active ingredient for improving alcoholism.

A herbal composition for improving alcoholism, comprising: 50 to 75% by weight of an extract of Antrodia camphorata and 25 to 50% of one of the licorice liquor extracts.

The herbal composition for improving alcoholism in the present invention, wherein the extract of Antrodia camphorata is extracted from a solid sample of Antrodia camphorata, and is obtained by extracting an aqueous solution of ethanol having a volume percentage of 95%.

The herbal composition for improving alcoholism in the present invention, wherein the extract of the licorice liquor is extracted from a licorice root sample which is subjected to honey baking, and is an ethanol aqueous solution having a volume percent concentration of 95%. Extracted to obtain.

The herbal composition for improving alcoholism in the present invention, wherein the licorice extract is obtained by a method comprising the steps of: mixing the licorice root sample and a condensed honey in a weight ratio of 4:1; Mixing the licorice root sample and the nectar mixture together at 110-130 ° C for 4-5 hours, and frying at 110-130 ° C; and extracting the honey-dried licorice with the aqueous ethanol solution Root sample.

A use of the above-mentioned herbal composition of the above-mentioned herbal medicine is applied to the preparation of a medicament for improving alcoholism, and the herbal composition is administered to a desired individual to improve the alcohol poisoning phenomenon of the desired ascending object.

The use of the herbal composition of the present invention, wherein the Chinese herbal compound composition is administered to the desired individual 20 to 40 minutes before the intake of the desired individual, and the dosage is 10-40 mg per administration. Preferably, the herbal composition is administered to the individual in need thereof 30 minutes prior to the ingestion of the desired individual, and each dose is 30 mg/kg body weight.

The herbal composition for improving alcoholism in the present invention is achieved by reducing the cost of improving the active ingredient of alcoholism by adding the extract of licorice liquor. Improve the cost-effectiveness of active ingredients in alcoholism.

The use of the herbal composition of the present invention is to increase the activity of alcohol dehydrogenase in cells and accelerate the metabolic intake of ethanol by using the composition ratio of the extract of Antrodia camphorata and the extract of Zhigancao wine, thereby improving ethanol intake. The problems of exercise coordination, sleep time and the like are the effects of the present invention.

The use of the herbal composition of the present invention is to increase the activity of antioxidant enzymes in the cells by the composition ratio of the extract of the burdock wine and the extract of the licorice liquor, so that the liver cells can resist the damage of free radicals, and Achieve the effect of improving liver damage caused by ethanol intake.

Figure 1: Bar graph of the relative drop rotation of the B0~B3 mice over time.

Figure 2: A histogram of the relative recovery of Orthonormal Reflex in mice in Groups B0 to B3.

Figure 3: Bar graph of alcohol dehydrogenase activity in relative cells of mice in Groups C0 to C3.

Figure 4: Bar graph of glutathione S transferase activity in the relative serum of mice in groups D0 to D3.

Figure 5: Bar graph of glutathione peroxidase activity in relative cells of mice in groups D0 to D3.

The above and other objects, features and advantages of the present invention will become more <RTIgt; The compound composition comprises: 50% to 75% by weight of an extract of Antrodia camphorata wine and 25~50% of one of the licorice liquor extracts, wherein the extract of the Antrodia camphorata wine and the extract of the licorice liquor The ratio can improve the alcoholism of the individual.

The extraction method of the Antrodia camphorata wine extract and the extract of the Licorice wine is the invention Those having ordinary knowledge in the art can adjust according to requirements, and are not limited herein. In detail, the extract of Antrodia camphorata is extracted from a solid sample of Antrodia camphorata, for example, extracted by a 95% by volume aqueous solution of ethanol; and the extract of the Licorice extract is extracted from a candied fruit. The licorice root sample is obtained, for example, by extracting from a 95% by volume aqueous solution of ethanol, and the candied licorice root sample can be obtained by a method comprising the following steps: for example, a weight ratio of 4:1 Mixing a licorice root sample and a refined honey (heated at 116-119 ° C to a water content of 10 to 13%), and the licorice root sample and the mixture of the nectar are combined at 110~ Stir at 130 ° C for 4 to 5 hours, and finally stir fry at 110 ~ 130 ° C. In this embodiment, the A. angustifolia fruit body sample and the candied licorice root sample (500 g in total) are mixed, and then extracted with 1 L of an aqueous ethanol solution (95% by volume) at 70 to 90 ° C. The Chinese herbal compound composition can be obtained after 10 hours, followed by vacuum filtration, concentration under reduced pressure, and lyophilization.

The herbal composition of the present invention can effectively improve the physiological state caused by ethanol intake, and thus can be used as an active ingredient for improving alcoholism. For example, the Chinese herbal compound composition can be applied to prepare a drug or health food for improving alcoholism. The Chinese herbal compound composition is combined with a pharmaceutically acceptable carrier or excipient to form a pharmaceutical composition, wherein the Chinese herbal compound composition can be prepared into any convenient form such as a tablet, a capsule or a powder. , granules or liquids, etc., or the combination of the Chinese herbal compound and other foods or beverages, for oral administration to the organism in a form suitable for consumption.

In addition, the Chinese herbal compound composition can be administered to a desired individual, for example, 20 to 40 minutes before the desired individual takes in ethanol, and the dose is 10-40 mg per dose. The kilogram of body weight, preferably, can be administered to the desired individual 30 minutes before the ingestion of the desired individual, and the dosage is 30 mg/kg body weight per administration, so that the active ingredient contained in the Chinese herbal compound composition can be obtained. The desired individual acts in the body and can improve the alcoholism of the desired individual.

The following is a preparation of the Chinese herbal compound composition containing the different ratios of the extract of Antrodia camphorata and the extract of Zhigancao wine, and the behavioral test and the physiological activity test are successively confirmed that the Chinese herbal compound composition of the invention has the activity of improving alcoholism:

(A) Preparation of Chinese herbal compound composition

In this test, a sample of the body of A. angustifolia and a root sample of candied licorice (500 g) as shown in Table 1 were taken, and extracted with 1 L of an aqueous ethanol solution (95% by volume) to obtain the first test. A1~A3 group of Chinese herbal compound composition; the licorice root sample was substituted for the root sample of candied licorice in group A1~A3, and the mixture was extracted with ethanol aqueous solution to obtain the first A1'~A3' of the experiment. The herbal composition of the group was extracted; in addition, 500 g of the extract of Antrodia camphorata fruit body was extracted with 1 L of an aqueous ethanol solution (95% by volume) to obtain an extract of Antrodia camphorata as a control group (Group A0).

The follow-up trials used ICR male mice (between 18 and 22 grams) purchased from the National Experimental Animal Center of the National Experimental Research Institute, and Wistar male rats (8 weeks old and above, weighing between 180 and 190 grams). ) as a test animal. The aforementioned test animals were housed in the Animal Center of Daren University of Science and Technology, and maintained an animal room at room temperature of 24 ± 2 ° C (12 hours of light time and dark time), and were free to eat and drink.

(B) Behavioral testing

In this test, the motor coordination of the aforementioned ICR male mice was observed by a rotarod test, and the oral intake of each group of ICR mice was as follows. The herbal composition of the herbal medicine (intake dose of 30 mg/kg) was orally administered with an aqueous solution of ethanol (30% by volume, and the ingested dose was 100 mL/kg) for 30 minutes, and then forced each group of ICR mice to rotate. Reverse motion was performed on rods (rotarod, XZC-4B, 16 rpm) and each group of ICR mice was recorded for latency to fall.

In addition, the drop rotarod of ICR mice which was only taken orally in an aqueous solution of ethanol (30% by volume, and the ingested dose was 100 mL/kg) was used as a positive control group, and only distilled water was orally taken (the ingestion dose was 100 mL). /kg) The ITR mouse's drop rotation rod duration was used as the negative control group, and the relative drop rotation rod duration (%) of each group of ICR mice was calculated as shown in the following figure, and the results are shown in Fig. 1.

(test group - positive control group) / (negative control group - positive control group) × 100% (Formula 1)

Please refer to Fig. 1 for the above-mentioned oral ingestion of the extract of A. serrata and the group A0~A3 of the A1~A3 group of ICR mice. The positive control group of the aqueous ethanol solution, in which the relative drop rotation rods of the B2 and B3 groups of the A2 and B3 groups of the herbal extracts of the A2 and A3 groups were ingested for about 41% and 43%, respectively. Display the combination of herbal extracts in group A2 and group A3 The effect of extracting materials on the improvement of motor coordination abnormalities caused by ethanol intake was the most significant; in addition, compared with the extract of Antrodia camphorata in Group A0, the A1'~A3' group of Chinese herbal medicines extracted from the sample of A. angustifolia and the roots of licorice roots Compound extracts have a poor effect on improving motor coordination abnormalities caused by ethanol uptake, so no further tests are performed.

The sleep time of the aforementioned ICR male mice was observed by the right reflex test, and the ICR mice of each group were orally ingested as in the second table of the herbal compound composition (the ingestion dose was 30 mg/ Kg), continued to take an aqueous solution of ethanol (55% by volume, ingestion dose of 100 mL/kg) for 30 minutes, followed by loss of righting reflex in each group of ICR mice. Group ICR mice were placed face down in the cage and the time to regain of righting reflex was recorded for each group of ICR mice.

In addition, in the case of ICR mice that only received oral ethanol solution (55% by volume, ingestion dose of 100 mL/kg), the time of returning regurgitation was taken as the positive control group, and only distilled water was taken orally (intake dose). The response of the ICR mice to 100 mL/kg) was taken as the negative control group, and the time required to restore the Orthonormal reflex of each group of ICR mice was calculated as the following formula (2). The results are shown in Fig. 2. Show.

(test group - negative control group) / (positive control group - negative control group) × 100% (Formula 2)

Referring to Fig. 2, the response to the anteroposterior reflex of the B0~B3 ICR mice of the A0 yoghurt extract and the A1~A3 medicinal compound composition in advance is shorter than the time only. The positive control group for oral intake of ethanol aqueous solution, in which the anterior reflexes of the B2 and B3 groups of the A2 and B3 groups of the A2 and A3 groups of the herbal extracts of the A2 group and the A3 group can be shortened by about 49%, respectively. 42% showed that the herbal extracts of the A2 and A3 groups had the most significant effect on improving the sleep time caused by ethanol intake.

(C) Determination of physiological activity

After ingesting ethanol, it will enter the liver via blood, and will continue to be oxidized by alcohol dehydrogenase (ADH) to acetaldehyde, and then dehydrogenated by acetaldehyde. The enzyme (acetaldehyde dehydrogenase, ALDH for short) is converted into acetic acid, so the activity of alcohol dehydrogenase in cells can be used as an indicator of ethanol metabolism. This experiment is to make oral intake of each group of Wistar rats. The herbal composition of the herbal medicine (intake dose of 30 mg/kg) was orally administered with an aqueous solution of ethanol (40% by volume, 2 mL/kg of the ingestion dose) for 30 minutes, and then the Wistar rats of each group were measured. Alcohol dehydrogenase activity.

At the same time, the alcohol dehydrogenase activity of Wistar rats, which only received oral ethanol solution (40% by volume, and the ingestion dose was 2 mL/kg), was used as a positive control group, and only distilled water was orally administered (intake dose was 2 mL/ The alcohol dehydrogenase activity of Wistar rats of kg) was used as a negative control group, and the relative alcohol dehydrogenase activity (%) of each group of Wistar rats was calculated by the following formula 3, and the results are shown in Fig. 3.

(test group - positive control group) / (negative control group - positive control group) × 100% (Formula 3)

Please refer to Figure 3 for the alcohol dehydrogenase activity of the C0~C3 Wistar rats in the A0 to C3 group and the A1~A3 group in the A1~A3 group. In the positive control group of the aqueous solution of ethanol, the alcohol dehydrogenase activity of the C2 and C3 groups of the A2 and A3 groups of the herbal extracts of the A2 and A3 groups can be increased by about 64% and 88%, respectively. It shows that the intake of the herbal extracts of the A2 and A3 groups can effectively increase the activity of the alcohol dehydrogenase in the cells, thereby accelerating the metabolism of ethanol in the body.

(D) liver protection effect

In addition, ethanol intake is the cause of liver damage, so each group of Wistar rats was orally ingested, such as the herbal composition of the fourth table (intake dose of 30 mg / kg), continued to take oral ethanol solution (volume) for 30 minutes. The percentage concentration was 40%, the ingestion dose was 2 mL/kg), and then the glutathione S-transferase (GST) activity in the serum of each group of Wistar rats and the glutathion in the cells were measured. Glutathione peroxidase (GSH-PX) activity.

This test is the same as glutathione S-transferase activity (or glutathione peroxidase activity) in Wistar rats with oral ethanol (40% by volume, 2 mL/kg). As a positive control group, glutathione S transferase activity (or glutathione peroxidase activity) of Wistar rats, which only received distilled water (intake dose of 2 mL/kg), was used as a negative control group. The relative glutathione S transferase activity (%) of each group of Wistar rats was calculated by the following formula 4 and the relative glutathione peroxidase activity (%) of each group of Wistar rats was calculated as follows: The results are shown in Figures 4 and 5, respectively.

(test group - negative control group) / (positive control group - negative control group) × 100% (Formula 4)

(test group - positive control group) / (negative control group - positive control group) × 100% (Equation 5)

Glutathione S transferase is an important enzyme for detoxification of the liver. When the liver is damaged, glutathione S transferase is released into the blood, thus in the serum. The increase in glutathione S transferase activity can be used as an indicator of liver damage, so as shown in Fig. 4, the intake of the herbal extract of the A2 and A3 groups can effectively reduce glutathione in serum. The activity of the S-transferase showed that the intake of the herbal extract of the A2 and A3 groups improved the liver damage caused by the ethanol intake.

In addition, as shown in Fig. 5, the intake of the herbal extracts of the A2 and A3 groups can also effectively increase the activity of glutathione peroxidase in the cells, and glutathione peroxidase is An important enzyme that protects organisms from oxidative damage, indicating that the intake of herbal extracts in Groups A2 and A3 may increase the activity of antioxidant enzymes, so that liver cells can resist free radical damage. It can improve liver damage caused by ethanol intake.

In summary, the herbal composition for improving alcoholism in the present invention reduces the cost of improving the active ingredient of alcoholism by adding the extract of licorice liquor, and can achieve the cost performance of improving the active ingredient of alcoholism. efficacy.

Furthermore, the use of the herbal composition of the present invention is to increase the activity of the alcohol dehydrogenase in the cell and accelerate the metabolic intake of ethanol by using the composition ratio of the extract of the burdock wine and the extract of the licorice liquor. Improving the coordination of exercise caused by ethanol intake, sleep time and the like are the effects of the present invention.

In addition, the use of the herbal composition of the present invention is to increase the activity of the antioxidant enzymes in the cells by the composition ratio of the extract of the burdock wine and the extract of the licorice liquor, so that the liver cells can resist the damage of free radicals. The effect of improving liver damage caused by ethanol intake can be achieved.

While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.

Claims (7)

A herbal composition for improving alcoholism, comprising: 50 to 75% by weight of an extract of Antrodia camphorata and 25 to 50% of one of the licorice liquor extracts. The herbal composition for improving alcoholism according to the first aspect of the patent application, wherein the extract of the Antrodia camphorata is extracted from a sample of the body of the Antrodia camphorata, and the volume is 95% by volume. Extracted from an aqueous ethanol solution. The herbal composition for improving alcoholism, as described in claim 1, wherein the extract of the licorice liquor is extracted from a root sample of a candied licorice, and the concentration is 95% by volume. It is obtained by extraction with an aqueous solution of ethanol. The herbal composition for improving alcoholism according to the third aspect of the patent application, wherein the candied licorice root sample is obtained by the method comprising the steps of: mixing a licorice in a weight ratio of 4:1; The root sample and a sweetened honey; and the mixture of the licorice root sample and the sweetened honey together are simmered at 110-130 ° C for 4-5 hours, and the stir-fry is performed at 110-130 ° C. The use of the herbal composition according to any one of claims 1 to 4 for the preparation of a medicament for improving alcoholism, wherein the herbal composition is administered to a desired individual to improve the Alcoholism in the required lifting object. The use of the herbal composition according to claim 5, wherein the Chinese herbal compound composition is administered to the desired individual 20 to 40 minutes before the alcohol intake of the desired individual, and each time the administration is performed. The dose is 10~40 mg/kg body weight. The use of the herbal composition according to claim 6, wherein the herbal composition is administered to the individual required 30 minutes before the alcohol intake of the desired individual, and the dose is administered each time. 30 mg / kg body weight.