TWI582421B - Simplified chemical sensor, its sensing method and carrier for reactants - Google Patents
Simplified chemical sensor, its sensing method and carrier for reactants Download PDFInfo
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本發明係關於一種化學物質檢測裝置及方法，特別係關於一種可供使用者簡易操作的檢測裝置及方法。The present invention relates to a chemical substance detecting device and method, and more particularly to a detecting device and method for easy operation by a user.
隨著消費者意識抬頭且食安問題頻仍，許多消費者非常想了解所購買的食品、保養品是否含有有毒物質或所宣稱的有效成分，這通常需藉由專業的化學物質檢測過程來解答，例如每日食用的蔬果是否有農藥殘留，或經常食用的各種保健食品是否具有有效成分等。這些化學物質的檢測過程通常是在實驗室由專業人員利用精密儀器執行，例如透過能呈色的反應物及精密的光譜儀器來進行檢測。但是，將待測物送至實驗室進行檢驗的成本極高，因此一般消費者不太可能時常送驗；而另一方面，一般民生物品的消費者也不太可能自備專業儀器，且通常不具有調配試劑的能力，因此，利用專業儀器進行化學物質檢測，顯難完全滿足一般消費者的需求。With the rise of consumer awareness and frequent food safety problems, many consumers are keen to know whether the foods and skin care products they purchase contain toxic substances or claimed active ingredients, which are usually answered by a professional chemical detection process. For example, whether the fruits and vegetables that are eaten daily have pesticide residues, or whether various health foods that are frequently consumed have active ingredients. The detection of these chemicals is usually carried out in the laboratory by professionals using precision instruments, such as through color-developable reactants and sophisticated spectroscopic instruments. However, the cost of sending the test object to the laboratory for inspection is extremely high, so it is unlikely that the average consumer will send the test from time to time; on the other hand, consumers of ordinary civilian products are less likely to bring their own professional instruments, and usually It does not have the ability to prepare reagents. Therefore, it is difficult to fully meet the needs of ordinary consumers by using chemical instruments for professional substances.
隨著科技的進步，智慧型手持裝置，例如智慧型手機或平板電腦，幾乎已成為人手一台的日常生活輔助工具，這些智慧型手持裝置具有可運算數據的處理器，通常也搭載了光源及感光元件，因此，如何結合智慧型手持裝置，使消費者能輕易地、隨身地自行執行感興趣的化學物質檢測，實係值得本領域人士思量的。With the advancement of technology, smart handheld devices, such as smart phones or tablets, have almost become a daily life aid for human hands. These smart handheld devices have a processor that can calculate data, usually with a light source. Photosensitive components, therefore, how to combine smart handheld devices, so that consumers can easily and automatically carry out the detection of chemical substances of interest, it is worthy of consideration in the field.
本發明之主要目的係提供一種可供使用者簡易操作的化學物質檢測裝置及方法。The main object of the present invention is to provide a chemical substance detecting device and method which can be easily operated by a user.
為了達成前述目的，本發明提供一種化學物質簡易檢測裝置，用以檢測一待測物中是否含有待測化學物質，該化學物質簡易檢測裝置包括一檢測試劑載體、一光照手段及一智慧型手持裝置，該檢測試劑載體具有一初始狀態及一檢測狀態，該檢測試劑載體係載有一可在檢測狀態時與該待測物接觸且可供與該待測化學物質反應之第一反應物、及一可供與該第一反應物或一第一產物進行呈色反應之第二反應物，該第一產物係該第一反應物與待測化學物質反應後之產物，當該檢測試劑載體處於初始狀態時，該第二反應物不接觸該第一反應物及可能存在的第一產物，當檢測試劑載體處於檢測狀態時，該第二反應物接觸該第一反應物或可能存在的第一產物；其中，該第一、第二反應物接觸後的呈色不同於該第一產物與第二反應物接觸後的呈色；該光照手段是用以在所述檢測狀態時對該檢測試劑載體提供光線；該智慧型手持裝置具有一運算單元及一感光元件電連接於該運算單元，該感光元件係用以感測自該檢測試劑載體反射或折射之光線並發出感光訊號，運算單元根據該感光訊號判斷該待測物是否含有待測化學物質。In order to achieve the foregoing object, the present invention provides a simple chemical detection device for detecting whether a test substance contains a chemical substance to be tested, and the simple detection device for the chemical substance comprises a detection reagent carrier, an illumination means and a smart hand-held device. The detection reagent carrier has an initial state and a detection state, and the detection reagent carrier carries a first reactant which is in contact with the analyte and is reactive with the chemical substance to be tested, and a second reactant for color reaction with the first reactant or a first product, the first product being a product of the first reactant reacted with the chemical to be tested, when the detection reagent carrier is In the initial state, the second reactant does not contact the first reactant and the first product that may be present, and when the detection reagent carrier is in the detection state, the second reactant contacts the first reactant or may be first a product; wherein the color of the first and second reactants after contact is different from the color of the first product after contact with the second reactant; Providing light to the detection reagent carrier in the detection state; the smart handheld device has an operation unit and a photosensitive element electrically connected to the operation unit, the photosensitive element is configured to sense reflection or refraction from the detection reagent carrier The light source emits a light sensing signal, and the arithmetic unit determines whether the object to be tested contains the chemical substance to be tested according to the light sensing signal.
為了達成前述目的，本發明亦提供一種化學物質簡易檢測方法，包含以下步驟：（A）將待測物投入該檢測試劑載體並依序與該第一反應物及該第二反應物接觸；（B）利用該光照手段對該檢測試劑載體提供光線；（C）利用該智慧型手持裝置的感光元件感測自該檢測試劑載體反射或折射之光線並發出感光訊號；以及（D）利用該智慧型手持裝置的運算單元根據該感光訊號判斷該待測物是否含有待測化學物質。In order to achieve the foregoing object, the present invention also provides a simple method for detecting a chemical substance, comprising the steps of: (A) putting a test object into the test reagent carrier and sequentially contacting the first reactant and the second reactant; B) using the illumination means to provide light to the detection reagent carrier; (C) sensing, by the photosensitive element of the smart handheld device, light reflected or refracted from the detection reagent carrier and emitting a light sensing signal; and (D) utilizing the wisdom The arithmetic unit of the handheld device determines whether the object to be tested contains the chemical substance to be tested based on the light sensing signal.
為了達成前述目的，本發明還提供一種檢測試劑載體，用以檢測一待測物中是否含有待測化學物質，該檢測試劑載體具有一初始狀態及一檢測狀態，該檢測試劑載體係載有一可在檢測狀態時與該待測物接觸且可供與該待測化學物質反應之第一反應物、及一可供與該第一反應物或一第一產物進行呈色反應之第二反應物，該第一產物係該第一反應物與待測化學物質反應後之產物，當該檢測試劑載體處於初始狀態時，該第二反應物不接觸該第一反應物及可能存在的第一產物，當檢測試劑載體處於檢測狀態時，該第二反應物接觸該第一反應物或可能存在的第一產物；其中，該第一、第二反應物接觸後的呈色不同於該第一產物與第二反應物接觸後的呈色。In order to achieve the foregoing object, the present invention further provides a detection reagent carrier for detecting whether a test substance contains a chemical substance to be tested, the detection reagent carrier has an initial state and a detection state, and the detection reagent carrier carries a a first reactant that is in contact with the analyte and is reactive with the chemical to be tested, and a second reactant that is reactive with the first reactant or a first product in a state of detection The first product is a product of the reaction of the first reactant with the chemical substance to be tested, and when the detection reagent carrier is in an initial state, the second reactant does not contact the first reactant and the first product that may be present. When the detection reagent carrier is in the detection state, the second reactant contacts the first reactant or the first product that may be present; wherein the color of the first and second reactants after contact is different from the first product Coloration after contact with the second reactant.
為了達成前述目的，本發明更提供一種化學物質簡易檢測裝置，用以檢測一待測物中是否含有待測化學物質，該化學物質簡易檢測裝置包括一檢測試劑載體、一光照手段及一智慧型手持裝置，該檢測試劑載體具有一初始狀態及一檢測狀態，該檢測試劑載體係載有一可在檢測狀態時與該待測物接觸且可供與該待測化學物質反應之第一反應物、一可供與該第一反應物或一第一產物進行反應之第二反應物、及一可供與一第二產物進行呈色反應之第三反應物，該第一產物係該第一反應物與待測化學物質反應後之產物，該第二產物係該第二反應物與第一反應物或第一產物反應後之產物，當該檢測試劑載體處於初始狀態時，該第二反應物不接觸該第一反應物及可能存在的第一產物，當檢測試劑載體處於檢測狀態時，該第二反應物接觸該第一反應物或可能存在的第一產物，且該第三反應物可接觸可能存在的第二產物；其中，該第三反應物未與第二產物接觸的呈色不同於該第三反應物與第二產物接觸後的呈色；該光照手段用以在所述檢測狀態時對該檢測試劑載體提供光線；該智慧型手持裝置具有一運算單元及一感光元件電連接於該運算單元，該感光元件係用以感測自該檢測試劑載體反射或折射之光線並發出感光訊號，運算單元根據該感光訊號判斷該待測物是否含有待測化學物質。In order to achieve the foregoing object, the present invention further provides a simple chemical detection device for detecting whether a test substance contains a chemical substance to be tested, and the simple detection device for the chemical substance comprises a detection reagent carrier, an illumination means and a smart type. The handheld device has an initial state and a detection state, and the detection reagent carrier carries a first reactant that can be in contact with the analyte and can react with the chemical to be tested in a detection state, a second reactant for reacting with the first reactant or a first product, and a third reactant for color reaction with a second product, the first product being the first reaction a product of the reaction with the chemical substance to be tested, the second product being a product of the second reactant reacted with the first reactant or the first product, and when the detection reagent carrier is in an initial state, the second reactant Not contacting the first reactant and the first product that may be present, the second reactant contacting the first reactant or possibly present when the detection reagent carrier is in the detection state a product, and the third reactant is contactable with a second product that may be present; wherein the color of the third reactant that is not in contact with the second product is different from the color of the third reactant after contact with the second product The illumination means is configured to provide light to the detection reagent carrier during the detection state; the smart handheld device has an operation unit and a photosensitive element electrically connected to the operation unit, wherein the photosensitive element is used for sensing The light reflected or refracted by the reagent carrier is detected and a light-sensing signal is emitted, and the operation unit determines whether the object to be tested contains the chemical substance to be tested according to the light-sensing signal.
為了達成前述目的，本發明又提供一檢測試劑載體，具有一初始狀態及一檢測狀態，該檢測試劑載體係載有一可在檢測狀態時與該待測物接觸且可供與該待測化學物質反應之第一反應物、一可供與該第一反應物或一第一產物進行反應之第二反應物、及一可供與一第二產物進行呈色反應之第三反應物，該第一產物係該第一反應物與待測化學物質反應後之產物，該第二產物係該第二反應物與第一反應物或第一產物反應後之產物，當該檢測試劑載體處於初始狀態時，該第二反應物不接觸該第一反應物及可能存在的第一產物，當檢測試劑載體處於檢測狀態時，該第二反應物接觸該第一反應物或可能存在的第一產物，且該第三反應物可接觸可能存在的第二產物；其中，該第三反應物未與第二產物接觸的呈色不同於該第三反應物與第二產物接觸後的呈色。In order to achieve the foregoing object, the present invention further provides a detection reagent carrier having an initial state and a detection state, wherein the detection reagent carrier carries a chemical substance that can be contacted with the analyte and can be used in the detection state. a first reactant of the reaction, a second reactant available for reaction with the first reactant or a first product, and a third reactant for color reaction with a second product, the first reactant a product is a product of the reaction of the first reactant with a chemical substance to be tested, and the second product is a product of the second reactant reacted with the first reactant or the first product, when the detection reagent carrier is in an initial state. While the second reactant is not in contact with the first reactant and the first product that may be present, the second reactant contacts the first reactant or the first product that may be present when the detection reagent carrier is in the detection state, And the third reactant can be contacted with a second product that may be present; wherein the color of the third reactant that is not in contact with the second product is different from the color of the third reactant after contact with the second product.
如此，使用者僅需將待測物加入該檢測試劑載體，並依序接觸第一、第二反應物，或依序接觸第一、第二、第三反應物，再使用該智慧型手持裝置照相或攝影並進行分析，即可完成對該化學物質的檢測，達到供使用者簡易操作的目的。In this way, the user only needs to add the test object to the test reagent carrier, and sequentially contact the first and second reactants, or sequentially contact the first, second, and third reactants, and then use the smart handheld device. Photography or photography and analysis can complete the detection of the chemical substance for the purpose of easy operation by the user.
請見第1-2圖，所繪示者為本發明之化學物質簡易檢測裝置10之第一實施例，該化學物質簡易檢測裝置10包括一檢測試劑載體20、一光照手段30及一智慧型手持裝置40。該檢測試劑載體20可容置一未知是否含有待測化學物質的待測物9，光照手段30提供光線，最後藉由智慧型手持裝置40感測、判斷該待測物9是否含有待測化學物質，待測物9可為但不限於食品或保養品等樣品。1-2 is a first embodiment of the simple chemical detection device 10 of the present invention. The simple detection device 10 includes a detection reagent carrier 20, an illumination means 30, and a smart type. Handheld device 40. The detection reagent carrier 20 can accommodate a test object 9 that does not contain a chemical substance to be tested, and the illumination means 30 provides light. Finally, the smart handheld device 40 senses and determines whether the test object 9 contains the chemical to be tested. The substance, the object to be tested 9 may be, but not limited to, a sample such as a food or a skin care product.
請見第1圖及第2A圖，本實施例中，該檢測試劑載體20為一至少局部透光的容器並具有一容室23，該容室23的頂部具有一蓋子24，蓋子24可選擇性地自容室23頂部分離，當蓋子24蓋住該容室23時，容室23內部可呈水密性。圍構該容室23的面包括一第一表面25及一第二表面26，該第一表面25可為該容室23的底面，該第二表面26則可為該容室23的頂面，亦即該蓋子24的內面。該第一表面25可藉由塗佈、印刷等方式載有一第一反應物21，該第二表面26同樣可依相似方式載有一第二反應物22。在此說明的是，圖式中為了清楚地顯示，第一反應物21、第二反應物22被誇張地加厚，實質上兩者的厚度比例並不以圖式所示者為限。Referring to FIG. 1 and FIG. 2A, in the embodiment, the test reagent carrier 20 is a container that is at least partially transparent and has a chamber 23 having a cover 24 at the top of the chamber 23, and the cover 24 is selectable. The chamber 23 is separated from the top of the chamber 23, and when the lid 24 covers the chamber 23, the interior of the chamber 23 can be watertight. The surface surrounding the chamber 23 includes a first surface 25 and a second surface 26. The first surface 25 can be the bottom surface of the chamber 23, and the second surface 26 can be the top surface of the chamber 23. That is, the inner surface of the cover 24. The first surface 25 can be loaded with a first reactant 21 by coating, printing, etc., and the second surface 26 can also carry a second reactant 22 in a similar manner. Here, in the drawings, in order to clearly show that the first reactant 21 and the second reactant 22 are exaggeratedly thickened, substantially the thickness ratio of the two is not limited to the one shown in the drawings.
其中，第一反應物21是可與待測化學物質反應的物質，兩者可反應生成至少一第一產物；第二反應物22是可與第一反應物21或第一產物進行呈色反應的物質，亦即第二反應物22至少可與第一反應物21及第一產物其中一者進行呈色反應，所述呈色反應包括有色變無色、無色變有色、或是由第一呈色變為第二呈色，且第一、第二反應物21、22接觸後的呈色不同於第一產物與第二反應物22接觸後的呈色，藉由觀察檢測試劑載體20在檢測狀態時的呈色及/或呈色的變化率，即能判斷待測物9是否含有待測化學物質。Wherein, the first reactant 21 is a substance reactive with the chemical substance to be tested, and the two may react to form at least one first product; the second reactant 22 may be colored reaction with the first reactant 21 or the first product. The substance, that is, the second reactant 22, can at least react with the first reactant 21 and the first product in a color reaction including color change, colorlessness, colorless color change, or first The color changes to the second color, and the color of the first and second reactants 21, 22 after contact is different from the color of the first product after contact with the second reactant 22, and the detection reagent carrier 20 is detected by observation. The color change rate and/or the change rate of coloration in the state can determine whether the test object 9 contains the chemical substance to be tested.
檢測試劑載體20具有一初始狀態（如第2A圖）及一檢測狀態（如第2C圖），當檢測試劑載體20處於初始狀態時，第二反應物21不接觸第一反應物22，例如在本實施例中，第一、第二反應物21、22被塗佈於容室23的不同表面，亦即檢測試劑載體20在初始狀態時，第二反應物22不會進行呈色反應；當檢測試劑載體20處於檢測狀態時，第二反應物22接觸第一反應物21或第一產物，所述第一產物是第一反應物21與待測化學物質反應後之產物。第二反應物22在檢測狀態時究係接觸第一反應物21或第一產物，則視待測物9是否含有待測化學物質而定，當待測物9含有實質有效量的待測化學物質時，第一反應物21與待測化學物質反應成第一產物，因此第二反應物22在檢測狀態時會接觸第一產物；另一方面，當待測物9未含有待測化學物質時，不會產生第一產物，因此第二反應物22在檢測狀態時會接觸第一反應物21。The detection reagent carrier 20 has an initial state (such as FIG. 2A) and a detection state (such as FIG. 2C). When the detection reagent carrier 20 is in an initial state, the second reactant 21 does not contact the first reactant 22, for example, In this embodiment, the first and second reactants 21, 22 are coated on different surfaces of the chamber 23, that is, when the reagent carrier 20 is detected in the initial state, the second reactant 22 does not undergo a color reaction; When the detection reagent carrier 20 is in the detection state, the second reactant 22 contacts the first reactant 21 or the first product, which is the product of the reaction of the first reactant 21 with the chemical substance to be tested. When the second reactant 22 contacts the first reactant 21 or the first product in the detection state, depending on whether the analyte 9 contains the chemical to be tested, when the analyte 9 contains a substantial effective amount of the chemical to be tested In the case of a substance, the first reactant 21 reacts with the chemical substance to be tested to form a first product, so that the second reactant 22 contacts the first product in the detection state; on the other hand, when the analyte 9 does not contain the chemical substance to be tested At the time, the first product is not produced, so the second reactant 22 contacts the first reactant 21 in the detection state.
在可能的情況下，檢測試劑載體20還具有一預備狀態（如第2B圖），預備狀態是作為初始狀態與檢測狀態之間的過渡狀態，此時待測物9已與第一反應物21接觸，但兩者均尚未接觸第二反應物，藉此使第一反應物21與待測物9中可能存在的待測化學物質能預先進行反應。When possible, the detection reagent carrier 20 also has a preliminary state (as shown in FIG. 2B), and the preliminary state is a transition state between the initial state and the detection state, at which time the analyte 9 has been reacted with the first reactant 21 Contact, but neither of them has contacted the second reactant, whereby the first reactant 21 and the chemical substance to be tested which may be present in the analyte 9 can be reacted in advance.
第一、第二反應物21、22的選用視待測化學物質而定。The selection of the first and second reactants 21, 22 depends on the chemical to be tested.
例如，若待測化學物質為澱粉酶，第一反應物21為可與澱粉酶反應的澱粉，第二反應物22為三碘陰離子（I3 - ）而可與澱粉進行呈色反應，若待測物中具有澱粉酶，則澱粉酶會將澱粉分解為麥芽糖，麥芽糖不與三碘陰離子進行呈色反應；反之，若待測物中不具有澱粉酶，則澱粉會與三碘陰離子進行呈色反應。For example, if the chemical substance to be tested is amylase, the first reactant 21 is a starch reactive with amylase, and the second reactant 22 is a triiodide anion (I 3 - ), which can react with starch to form a color reaction. If the amylase is detected in the test, the amylase will decompose the starch into maltose, and the maltose will not react with the triiodide; if the sample does not have amylase, the starch will color with the triiodide. reaction.
又例如，若待測化學物質為油脂分解酵素，則第一反應物21為可與油脂分解酵素反應的油脂，第二反應物22為酚紅，若待測物中含有油脂分解酵素，則油脂分解酵素會將油脂分解為甘油及脂肪酸，脂肪酸與酚紅進行呈色反應；反之，若待測物中不含油脂分解酵素，油脂不會分解成脂肪酸，酚紅不會進行呈色反應。For example, if the chemical substance to be tested is a lipolysis enzyme, the first reactant 21 is a fat which can react with a lipolysis enzyme, and the second reactant 22 is a phenol red. If the analyte contains a lipolytic enzyme, the fat is Decomposing enzymes will decompose oil into glycerol and fatty acids, and fatty acids react with phenol red; on the other hand, if the analyte does not contain oil-decomposing enzymes, the oil will not decompose into fatty acids, and phenol red will not undergo a color reaction.
又或者，若待測化學物質為蛋白質分解酵素，則第一反應物21為可與蛋白質分解酵素反應的蛋白質，第二反應物22為考馬斯亮藍，若待測物中含有蛋白質分解酵素，則蛋白質會被蛋白質分解酵素分解；若待測物未含有蛋白質分解酵素，則蛋白質會與考馬斯亮藍進行呈色反應。Alternatively, if the chemical substance to be tested is a proteolytic enzyme, the first reactant 21 is a protein reactive with proteolytic enzymes, and the second reactant 22 is Coomassie brilliant blue, and if the analyte contains a proteolytic enzyme, The protein is broken down by the proteolytic enzyme; if the analyte does not contain a proteolytic enzyme, the protein will react with Coomassie Brilliant Blue.
其他可能的檢測場合例如，對於麩質不耐症或乳糖不耐症的人來說，食品中是否含有麩質或乳糖是這些人想要瞭解的，因此檢測試劑載體的第一反應物可為麩質分解酵素或乳糖分解酵素，第二反應物為可與麩質分解產物或乳糖分解產物進行呈色反應的呈色劑，據此檢測食品中是否含有麩質或乳糖。惟需說明的是，適用於本檢測裝置的待測化學物質並不以上述者為限。Other possible testing occasions For example, for people with gluten intolerance or lactose intolerance, whether the food contains gluten or lactose is what these people want to know, so the first reactant for detecting the reagent carrier can be The gluten-decomposing enzyme or lactose-degrading enzyme, and the second reactant is a coloring agent which can react with gluten decomposition products or lactose decomposition products to determine whether the food contains gluten or lactose. It should be noted that the chemical substances to be tested that are suitable for the detection device are not limited to the above.
該光照手段30是用以對該檢測試劑載體20提供光線，如第1圖及第3圖所示的第一實施例中，光照手段30為設於該智慧型手持裝置40的LED。於其他可能的實施例中，該光照手段30可為其他光源（如第5圖所示），例如日光燈、檯燈、投射燈、手電筒、雷射或日光，這些光源可能是環境光源，或是專為檢測所設置的光源。The illumination means 30 is for providing light to the detection reagent carrier 20. In the first embodiment shown in Figs. 1 and 3, the illumination means 30 is an LED provided in the smart handheld device 40. In other possible embodiments, the illumination means 30 can be other light sources (as shown in FIG. 5), such as fluorescent lamps, table lamps, projection lamps, flashlights, lasers or daylight, which may be ambient light sources or To detect the set light source.
請見第3圖，該智慧型手持裝置40具有一運算單元41及一感光元件42電連接於該運算單元41，如第4圖所示，該感光元件42係用以感測自該檢測試劑載體20反射的光線，特別是特定波長範圍的光線，並發出感光訊號，該感光元件42可為但不限為電荷耦合元件(Charge Coupled Device, CCD)、互補式金屬氧化物半導體(Complementary Metal-Oxide-semiconductor, CMOS)。該運算單元41根據該感光訊號判斷該待測物9是否含有待測化學物質，該運算單元41可為該智慧型手持裝置40的處理器，智慧型手持裝置40更可安裝有專用於進行檢測的應用程式(application, app)來執行檢測判斷。該智慧型手持裝置40的運算單元41可進一步利用該感光元件42來感測是否有特定波長的光線存在或光強度的變化率，據此推估待測化學物質的活性或濃度，並可輸出一判讀結果，例如以該智慧型手持裝置40的螢幕顯示出有害或安全的字樣，俾分別代表該化學物質是否存在或其含量多寡；在其他可能的實施例中，判讀結果可以是量化的數據、圖表或化學物質安全資料表等資訊。As shown in FIG. 3, the smart handheld device 40 has an operation unit 41 and a photosensitive element 42 electrically connected to the operation unit 41. As shown in FIG. 4, the photosensitive element 42 is used to sense the detection reagent. The light reflected by the carrier 20, in particular, the light of a specific wavelength range, and emits a light-sensing signal. The photosensitive element 42 can be, but is not limited to, a charge coupled device (CCD), a complementary metal oxide semiconductor (Complementary Metal- Oxide-semiconductor, CMOS). The computing unit 41 determines whether the object to be tested 9 contains a chemical substance to be tested according to the light sensing signal. The computing unit 41 can be a processor of the smart handheld device 40, and the smart handheld device 40 can be installed with a dedicated device for detecting The application (application, app) to perform the detection and judgment. The operation unit 41 of the smart handheld device 40 can further utilize the photosensitive element 42 to sense whether there is a specific wavelength of light or a rate of change of light intensity, thereby estimating the activity or concentration of the chemical to be tested, and outputting As a result of the interpretation, for example, the screen of the smart handheld device 40 displays a harmful or safe word, which indicates whether the chemical substance exists or its content, respectively; in other possible embodiments, the interpretation result may be quantified data. Information such as charts, chemical safety data sheets, etc.
為了侷限進入該感光元件42的光線的波長，提高該感光元件42的專一性，該化學物質簡易檢測裝置10更可包含有一濾光片50，可供特定波長的光線進入該感光元件42，該濾光片50設於該檢測試劑載體20與該智慧型手持裝置40的感光元件42之間即有功效，如第1圖所示的第一實施例中，該濾光片50設於該智慧型手持裝置40的感光元件42前方，於其他可能的實施例中，該濾光片50可設於該檢測試劑載體20的一側面，或獨立置放。In order to limit the wavelength of the light entering the photosensitive element 42 and improve the specificity of the photosensitive element 42, the chemical simple detecting device 10 further includes a filter 50 for allowing light of a specific wavelength to enter the photosensitive element 42. The filter 50 is disposed between the detection reagent carrier 20 and the photosensitive element 42 of the smart handheld device 40. In the first embodiment shown in FIG. 1, the filter 50 is disposed in the wisdom. In front of the photosensitive element 42 of the handheld device 40, in other possible embodiments, the filter 50 may be disposed on one side of the detection reagent carrier 20 or placed separately.
為了使更多有效光線進入該感光元件42，該化學物質簡易檢測裝置10更可包含有一反光片60，設於該檢測試劑載體20相反於該智慧型手持裝置40之一面。在其他可能的實施例中，智慧型手持裝置40與光照手段30是設於檢測試劑載體20的相對側（例如第5圖所示），此時感光元件42可感測經由檢測試劑載體20折射的光線，反光片60可省略不設。In order to allow more effective light to enter the photosensitive element 42, the chemical simple detecting device 10 further includes a retroreflective sheeting 60 disposed on the side of the detecting reagent carrier 20 opposite to the smart handheld device 40. In other possible embodiments, the smart handheld device 40 and the illumination means 30 are disposed on opposite sides of the detection reagent carrier 20 (for example, as shown in FIG. 5), at which time the photosensitive element 42 can sense refraction via the detection reagent carrier 20. The light, the reflective sheet 60 can be omitted.
利用上述化學物質簡易檢測裝置10，可以下述化學物質簡易檢測方法來進行待測化學物質的檢測，步驟包括：The chemical substance simple detecting device 10 can detect the chemical substance to be tested by the following simple chemical substance detecting method, and the steps include:
（A）將待測物9投入檢測試劑載體20並依序與第一反應物21及第二反應物22接觸；(A) introducing the analyte 9 into the detection reagent carrier 20 and sequentially contacting the first reactant 21 and the second reactant 22;
（B）利用光照手段30對檢測試劑載體20提供光線，例如使智慧型手持裝置40的LED發光，或直接使用環境光源提供所需光線；(B) providing light to the detection reagent carrier 20 by means of illumination 30, such as illuminating the LED of the smart handheld device 40, or directly providing the desired light using an ambient light source;
（C）利用智慧型手持裝置40的感光元件42感測自檢測試劑載體20反射或折射之光線並發出感光訊號，如果待測物9中含有待測化學物質，則檢測試劑載體20內會呈第一呈色，而若待測物9中不含待測化學物質，則檢測試劑載體20將會呈第二呈色，因此待測物9中是否含有待測化學物質，將會表現在感光元件42所發出的感光訊號的差異；以及(C) sensing the light reflected or refracted from the detection reagent carrier 20 by the photosensitive element 42 of the smart handheld device 40 and emitting a light sensing signal. If the analyte 9 contains the chemical substance to be tested, the detection reagent carrier 20 may be present. The first coloration, and if the analyte 9 does not contain the chemical to be tested, the detection reagent carrier 20 will have a second coloration, so whether the analyte 9 contains the chemical to be tested will be displayed in the photosensitive The difference in the sensible signals emitted by component 42;
（D）利用智慧型手持裝置40的運算單元41根據感光訊號判斷待測物9是否含有待測化學物質，例如根據該感光訊號所反應之波長或光強度變化率來進行判斷。(D) The arithmetic unit 41 of the smart handheld device 40 determines whether the object to be tested 9 contains the chemical substance to be tested based on the light sensing signal, for example, based on the wavelength or the rate of change of the light intensity reflected by the light sensing signal.
如此，該化學物質簡易檢測裝置10即可完成對該待測化學物質的檢測，實現可供使用者簡單操作的目的。In this way, the chemical substance simple detecting device 10 can complete the detection of the chemical substance to be tested, thereby achieving the purpose of simple operation by the user.
在第2A-2C圖所示的第一實施例中，為了令待測物9先後接觸第一、第二反應物21、22，第一、第二反應物21、22是塗佈於容室23的相對表面25、26，其中第2A圖所表現的檢測試劑載體20是處於一初始狀態，該第一反應物21不接觸該第二反應物22，且待測物9尚未投入該檢測試劑載體20。如第2B圖所示，此時待測物9投入直立的該檢測試劑載體20，則該檢測試劑載體20處於預備狀態，該第一反應物21與該待測物9接觸，若待測物9含有待測化學物質，則第一反應物21與待測化學物質可進行反應並產生至少一第一產物。如第2C圖所示，此時將該檢測試劑載體20倒立，倒立的過程可伴隨搖晃攪拌，使該第二反應物22接觸待測物9及可能存在的第一反應物21或第一產物，該第二反應物22可供與該第一反應物21或該第一產物進行呈色反應，該第一反應物21、第二反應物22接觸後的呈色不同於該第一產物與第二反應物22接觸後的呈色。In the first embodiment shown in FIG. 2A-2C, in order for the object to be tested 9 to sequentially contact the first and second reactants 21, 22, the first and second reactants 21, 22 are coated in the chamber. The opposite surface 25, 26 of 23, wherein the detection reagent carrier 20 represented by FIG. 2A is in an initial state, the first reactant 21 does not contact the second reactant 22, and the analyte 9 has not been charged with the detection reagent Carrier 20. As shown in FIG. 2B, when the test object 9 is put into the test reagent carrier 20 which is upright, the test reagent carrier 20 is in a preliminary state, and the first reactant 21 is in contact with the test object 9, if the test object is to be tested. 9 containing the chemical to be tested, the first reactant 21 and the chemical to be tested can be reacted to produce at least a first product. As shown in FIG. 2C, at this time, the detection reagent carrier 20 is inverted, and the process of inversion may be accompanied by shaking and stirring to bring the second reactant 22 into contact with the analyte 9 and the first reactant 21 or the first product which may be present. The second reactant 22 is available for color reaction with the first reactant 21 or the first product, and the color of the first reactant 21 and the second reactant 22 after contact is different from the first product and The color of the second reactant 22 after contact.
在第6圖所示的第二實施例中，第一、第二反應物21、22則是塗佈於容室23的相鄰表面，可透過翻轉檢測試劑載體20而使待測物9先後接觸第一、第二反應物21、22，或者透過搖晃檢測試劑載體20，使已接觸第一反應物21的待測物9將第二反應物22溶解。In the second embodiment shown in FIG. 6, the first and second reactants 21, 22 are coated on the adjacent surface of the chamber 23, and the object to be tested 9 can be passed through the inversion detection reagent carrier 20. Contacting the first or second reactants 21, 22, or by shaking the reagent carrier 20, the analyte 9 that has contacted the first reactant 21 dissolves the second reactant 22.
或者，如第7A-7C圖所示的第三實施例中，檢測試劑載體20可設計成具有一階部28，階部28設有一容置槽29而承載第一反應物21，檢測試劑載體20的底部則具有第二反應物22，當待測物滴入容置槽29與第一反應物21接觸一段時間之後，可傾斜檢測試劑載體20，使溶液流至其底部而接觸第二反應物22。Alternatively, as in the third embodiment shown in Figures 7A-7C, the detection reagent carrier 20 can be designed to have a first-order portion 28, the step portion 28 is provided with a receiving groove 29 for carrying the first reactant 21, and the detection reagent carrier The bottom of the bottom portion 20 has a second reactant 22, and after the test object is dropped into the accommodating groove 29 for a period of time with the first reactant 21, the reagent carrier 20 can be tilted to cause the solution to flow to the bottom portion to contact the second reaction. Object 22.
基於本發明的精神，該檢測試劑載體20可為其他變化，例如，如第8A圖所示的第四實施例中，為了比對檢測的結果，該檢測試劑載體20可具有一樣品槽231及一空白槽232，該樣品槽231及空白槽232內均具有第一反應物21及第二反應物22，該樣品槽231係供投入該待測物9，該空白槽232係供投入不含該待測化學物質的空白試劑8；接著，如第8B圖所示，各槽分別投入該待測物9及該空白試劑8使之各與該第一反應物21反應；之後，如第8C圖所示，密封該檢測試劑載體20並加以搖晃，使第二反應物22分別被溶解至待測物9及空白試劑8中，並觀察樣品槽231內是否發生呈色反應，而空白槽232則作為對照組做為參考，之後利用該光照手段30對該檢測試劑載體20提供光線，同時利用該智慧型手持裝置40的感光元件42分別感測自該樣品槽231及該空白槽232折射之光線並發出感光訊號，該智慧型手持裝置40的運算單元41根據自樣品槽231所測得的該感光訊號判斷該待測物9是否含有待測化學物質，並根據自空白槽232所測得的感光訊號進行校正或對照。In the spirit of the present invention, the detection reagent carrier 20 may be other variations. For example, in the fourth embodiment shown in FIG. 8A, the detection reagent carrier 20 may have a sample tank 231 and the result of the comparison detection. a blank slot 232, the sample tank 231 and the blank slot 232 each have a first reactant 21 and a second reactant 22, and the sample tank 231 is used for inputting the object to be tested 9, and the blank slot 232 is provided for input. a blank reagent 8 of the chemical substance to be tested; next, as shown in FIG. 8B, each of the tanks is separately charged with the analyte 9 and the blank reagent 8 to react with the first reactant 21; thereafter, as in the eighth As shown in the figure, the test reagent carrier 20 is sealed and shaken, so that the second reactant 22 is dissolved into the analyte 9 and the blank reagent 8, respectively, and whether a color reaction occurs in the sample tank 231, and the blank groove 232 is observed. Then, the detection reagent carrier 20 is provided with light by the illumination means 30, and the photosensitive element 42 of the smart handheld device 40 is respectively sensed from the sample slot 231 and the blank slot 232. Light and emit a sensation signal, the wisdom The operation unit 41 of the smart handheld device 40 determines whether the test object 9 contains the chemical substance to be tested according to the light sensing signal measured from the sample slot 231, and corrects or compares the light sensing signal measured from the blank slot 232. .
該檢測試劑載體20還可為其他設計，如第9A圖所示的本發明之第五實施例，該檢測試劑載體20的底部塗佈該第一反應物21，並具有被包覆材質291包覆的第二反應物22，藉此讓第一、第二反應物21、22在初始狀態時不直接接觸，該包覆材質291具有延遲的水溶性質，會在水溶液中延遲釋放該第二反應物22，接著，如第9B圖所示，當待測物9滴入該檢測試劑載體20後會先與該第一反應物21反應，且包覆材質291逐漸溶解，最後如第9C圖所示，第二反應物22被釋出，而可與待測物9中可能存在的第一反應物21或第一產物進行反應。於其他可能的實施例中，該包覆材質291可為一光可分解材料，以特定波長的光，例如雷射，照射該等包覆材質291而釋放該第二反應物22。The detection reagent carrier 20 can also be of other design, such as the fifth embodiment of the invention shown in FIG. 9A, the bottom of the detection reagent carrier 20 is coated with the first reactant 21, and has a coated material 291 package. The second reactant 22 is covered, whereby the first and second reactants 21, 22 are not directly contacted in an initial state, and the coating material 291 has a delayed water solubility, and the second reaction is delayed in the aqueous solution. Then, as shown in FIG. 9B, when the analyte 9 is dropped into the detection reagent carrier 20, it will first react with the first reactant 21, and the coating material 291 is gradually dissolved, and finally, as shown in FIG. 9C. It is shown that the second reactant 22 is released and can react with the first reactant 21 or the first product which may be present in the analyte 9. In other possible embodiments, the cladding material 291 can be a photo-decomposable material that emits the second reactant 22 by illuminating the cladding material 291 with a specific wavelength of light, such as a laser.
基於本發明的精神，該檢測試劑載體20更可具有一第三反應物27，該第三反應物27可與一第二產物進行呈色反應，該第二產物為該第二反應物22與該第一反應物21或第一產物進行反應後的產物。In accordance with the spirit of the present invention, the detection reagent carrier 20 may further have a third reactant 27, which may undergo a color reaction with a second product, the second product being the second reactant 22 and The first reactant 21 or the first product is subjected to a reaction product.
在可能的實施例中，第二產物是第二反應物與第一反應物進行反應後的產物，此時若待測物不含待測化學物質時，該第一產物不會被生成，則該第二反應物與該第一反應物反應後生成該第二產物，接著第三反應物也才可能與第二產物進行呈色反應；反之，如果待測物含有待測化學物質時，第一反應物會與待測化學物質反應生成第一產物，此時因第一反應物已被抑制或反應掉，因此該第二反應物22無法與第一反應物進行反應，故第二產物不會生成，第三反應物就不會進行呈色反應。In a possible embodiment, the second product is a product after the second reactant is reacted with the first reactant, and if the analyte is not formed, the first product is not formed. The second reactant reacts with the first reactant to form the second product, and then the third reactant is also likely to undergo a color reaction with the second product; conversely, if the analyte contains the chemical to be tested, A reactant reacts with the chemical substance to be tested to form a first product. At this time, since the first reactant has been inhibited or reacted, the second reactant 22 cannot react with the first reactant, so the second product is not Will generate, the third reactant will not undergo a color reaction.
在另一個可能的實施例中，第二產物是第二反應物與第一產物進行反應後的產物，此時若待測物不含待測化學物質時，第一產物不會被生成，連帶地第二產物也無法被生成，因此第三反應物無法進行呈色反應；反之，如果待測物含有待測化學物質時，第一產物及第二產物可被依序生成，此時第三反應物可與第二產物進行呈色反應。In another possible embodiment, the second product is a product of the second reactant reacted with the first product, and if the analyte is free of the chemical to be tested, the first product is not formed, The second product cannot be formed, so the third reactant cannot undergo a color reaction; conversely, if the analyte contains the chemical to be tested, the first product and the second product can be sequentially generated, and at this time, the third The reactants can be subjected to a color reaction with the second product.
如第10圖所示的第六實施例中，該檢測試劑載體20於初始狀態時，該第三反應物27與該第二反應物22置於該檢測試劑載體20的底部，當待測物9滴入容置槽29與第一反應物21接觸一段時間，產生該第一產物，之後可傾斜檢測試劑載體20，使溶液流至其底部而接觸第二反應物22，第二反應物22可與第一產物接觸產生該第二產物，該第二產物與該第三反應物27接觸進行呈色反應。In the sixth embodiment shown in FIG. 10, when the detection reagent carrier 20 is in an initial state, the third reactant 27 and the second reactant 22 are placed at the bottom of the detection reagent carrier 20, when the analyte is to be tested. 9 drops into the accommodating tank 29 to contact the first reactant 21 for a period of time to produce the first product, after which the reagent carrier 20 can be tilted and the solution is flowed to the bottom to contact the second reactant 22, the second reactant 22 The second product can be produced in contact with the first product, and the second product is contacted with the third reactant 27 for a color reaction.
第一、第二、第三反應物21、22、27的選用視待測化學物質而定。The selection of the first, second, and third reactants 21, 22, 27 depends on the chemical to be tested.
例如，若待測化學物質為可抑制乙醯膽鹼酯酶的農藥，例如有機磷劑或胺基甲酸鹽劑等乙醯膽鹼酯酶抑制劑，則第一反應物21可為乙烯膽鹼酯酶，第二反應物22為硫代乙醯膽鹼，第三反應物27為二硫代二硝基苯甲酸，其中乙醯膽鹼酯酶抑制劑可與乙醯膽鹼酯酶反應並抑制乙醯膽鹼酯酶的活性，而若乙醯膽鹼酯酶的活性並未被抑制，則乙醯膽鹼酯酶可與硫代乙醯膽鹼進行反應而產生硫代膽鹼，即第二產物，第三反應物27（二硫代二硝基苯甲酸）可與第二產物（硫代膽鹼）進行呈色反應而呈黃色。For example, if the chemical substance to be tested is a pesticide capable of inhibiting acetylcholinesterase, such as an acetylcholinesterase inhibitor such as an organophosphorus or an amine formate, the first reactant 21 may be a vinyl cholesteryl ester. Alkaline esterase, the second reactant 22 is thioethionol, and the third reactant 27 is dithiodinitrobenzoic acid, wherein the acetylcholinesterase inhibitor can react with acetylcholinesterase And inhibiting the activity of acetylcholinesterase, and if the activity of acetylcholinesterase is not inhibited, acetylcholinesterase can react with thiocholine to produce thiocholine. That is, the second product, the third reactant 27 (dithiodinitrobenzoic acid) can be colored in yellow with the second product (thiocholine).
又例如，若待測化學物質為油脂分解酵素的抑制劑，則第一反應物21為油脂分解酵素，第二反應物22為油脂，第三反應物27為酚紅，若待測物9含有油脂分解酵素的抑制劑，則油脂分解酵素與油脂分解酵素的抑制劑生成第一產物（去活性的油脂分解酵素），此時油脂無法被進一步分解為脂肪酸。若待測化學物質不含油脂分解酵素的抑制劑，油脂分解酵素可與油脂反應生成脂肪酸（第二產物22），而脂肪酸的存在可使酚紅呈黃色。For example, if the chemical substance to be tested is an inhibitor of oleolysis enzyme, the first reactant 21 is a lipolysis enzyme, the second reactant 22 is a fat, and the third reactant 27 is phenol red, and the analyte 9 contains Inhibitors of oil-decomposing enzymes, the inhibitor of oil-decomposing enzymes and oil-degrading enzymes produce a first product (deactivated oil-decomposing enzyme), at which point the oil cannot be further broken down into fatty acids. If the chemical to be tested does not contain an inhibitor of the oil-degrading enzyme, the oil-degrading enzyme can react with the oil to form a fatty acid (second product 22), and the presence of the fatty acid can make the phenol red yellow.
本創作雖以上揭實施例舉例說明，但不以此為限，其他未超脫本創作精神所作的結構置換或潤飾，仍應屬於本創作意欲保護之範疇。Although the present invention is exemplified by the above embodiments, it is not limited thereto, and other structural replacements or retouchings that are not beyond the spirit of the present invention should still belong to the scope of the creative intention.
8‧‧‧空白試劑
9‧‧‧待測物
10‧‧‧化學物質簡易檢測裝置
20‧‧‧檢測試劑載體
21‧‧‧第一反應物
22‧‧‧第二反應物
23‧‧‧容室
231‧‧‧樣品槽
232‧‧‧空白槽
24‧‧‧蓋子
25‧‧‧第一表面
26‧‧‧第二表面
27‧‧‧第三反應物
28‧‧‧階部
29‧‧‧容置槽
291‧‧‧包覆材質
30‧‧‧光照手段
40‧‧‧智慧型手持裝置
41‧‧‧運算單元
42‧‧‧感光元件
50‧‧‧濾光片
60‧‧‧反光片8‧‧‧ Blank reagent
9‧‧‧Test object
10‧‧‧Chemical substance simple detection device
20‧‧‧Test reagent carrier
21‧‧‧First reactant
22‧‧‧Second reactant
23‧‧‧ Room
231‧‧‧ sample trough
232‧‧‧Blank slot
24‧‧‧ cover
25‧‧‧ first surface
26‧‧‧ second surface
27‧‧‧ Third reactant
28‧‧‧
29‧‧‧ accommodating slots
291‧‧‧Cover material
30‧‧‧Light means
40‧‧‧Smart handheld device
41‧‧‧ arithmetic unit
42‧‧‧Photosensitive elements
50‧‧‧Filter
60‧‧‧Reflecting film
第1圖係本發明化學物質簡易檢測裝置之第一實施例的立體示意圖； 第2A-2C圖係本發明檢測試劑載體之第一實施例的使用狀態示意圖； 第3圖係本發明化學物質簡易檢測裝置之第一實施例中，智慧型手持裝置的示意圖； 第4圖示意本發明化學物質簡易檢測裝置之第一實施例中，智慧型手持裝置感測自該檢測試劑載體反射的光線之示意圖； 第5圖為本發明化學物質簡易檢測裝置之第二實施例的示意圖； 第6圖係本發明檢測試劑載體之第二實施例的示意圖； 第7A-7C圖係本發明檢測試劑載體之第三實施例的使用狀態示意圖； 第8A-8C圖係本發明檢測試劑載體之第四實施例的使用狀態示意圖； 第9A-9C圖係本發明檢測試劑載體之第五實施例的使用狀態示意圖； 第10A-10C圖係本發明檢測試劑載體之第六實施例的使用狀態示意圖。1 is a schematic perspective view of a first embodiment of a simple chemical detection device of the present invention; 2A-2C is a schematic view showing a state of use of the first embodiment of the detection reagent carrier of the present invention; and FIG. 3 is a simplified embodiment of the chemical substance of the present invention; In the first embodiment of the detecting device, a schematic diagram of the smart handheld device; and in the first embodiment of the simple chemical detecting device of the present invention, the intelligent handheld device senses the light reflected from the detecting reagent carrier. 5 is a schematic view of a second embodiment of a simple chemical detection device of the present invention; FIG. 6 is a schematic view showing a second embodiment of the detection reagent carrier of the present invention; and FIGS. 7A-7C are diagrams of the detection reagent carrier of the present invention. 3A-8C is a schematic view showing the state of use of the fourth embodiment of the test reagent carrier of the present invention; and FIG. 9A-9C is a schematic view showing the state of use of the fifth embodiment of the test reagent carrier of the present invention. Fig. 10A-10C is a schematic view showing the state of use of the sixth embodiment of the test reagent carrier of the present invention.
10‧‧‧化學物質簡易檢測裝置 10‧‧‧Chemical substance simple detection device
20‧‧‧檢測試劑載體 20‧‧‧Test reagent carrier
21‧‧‧第一反應物 21‧‧‧First reactant
22‧‧‧第二反應物 22‧‧‧Second reactant
23‧‧‧容室 23‧‧‧ Room
24‧‧‧蓋子 24‧‧‧ cover
25‧‧‧第一表面 25‧‧‧ first surface
26‧‧‧第二表面 26‧‧‧ second surface
30‧‧‧光照手段 30‧‧‧Light means
40‧‧‧智慧型手持裝置 40‧‧‧Smart handheld device
50‧‧‧濾光片 50‧‧‧Filter
60‧‧‧反光片 60‧‧‧Reflecting film
Claims (10)
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| TWM479417U (en) * | 2013-12-23 | 2014-06-01 | Brilliant Sensing Technology | Apparatus for residual pesticide detection |
| TW201440155A (en) * | 2013-02-19 | 2014-10-16 | Advanced Micro Fab Equip Inc | Endpoint detection device and method of plasma treatment process |
| TWM497667U (en) * | 2014-11-14 | 2015-03-21 | Ind Tech Res Inst | Portable real time detection device |
| TWM512707U (en) * | 2015-04-15 | 2015-11-21 | Yuanpei University Of Medical Technology | Simplified chemical sensor and carrier for reactants |
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|---|---|---|---|---|
| TW201440155A (en) * | 2013-02-19 | 2014-10-16 | Advanced Micro Fab Equip Inc | Endpoint detection device and method of plasma treatment process |
| TWM479417U (en) * | 2013-12-23 | 2014-06-01 | Brilliant Sensing Technology | Apparatus for residual pesticide detection |
| TWM497667U (en) * | 2014-11-14 | 2015-03-21 | Ind Tech Res Inst | Portable real time detection device |
| TWM512707U (en) * | 2015-04-15 | 2015-11-21 | Yuanpei University Of Medical Technology | Simplified chemical sensor and carrier for reactants |
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