CN105181681A - Pesticide detection device - Google Patents
Pesticide detection device Download PDFInfo
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- CN105181681A CN105181681A CN201410322517.2A CN201410322517A CN105181681A CN 105181681 A CN105181681 A CN 105181681A CN 201410322517 A CN201410322517 A CN 201410322517A CN 105181681 A CN105181681 A CN 105181681A
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- detection device
- pesticide detection
- pesticide
- microcontroller
- cuvette
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- 239000000575 pesticide Substances 0.000 title claims abstract description 55
- 238000001514 detection method Methods 0.000 title claims abstract description 45
- 239000000447 pesticide residue Substances 0.000 claims abstract 4
- 239000003905 agrochemical Substances 0.000 claims description 29
- 230000003287 optical effect Effects 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 10
- 230000002401 inhibitory effect Effects 0.000 claims description 8
- 108010022752 Acetylcholinesterase Proteins 0.000 claims description 6
- 102000057297 Pepsin A Human genes 0.000 claims description 6
- 108090000284 Pepsin A Proteins 0.000 claims description 6
- 229940111202 pepsin Drugs 0.000 claims description 6
- 238000013500 data storage Methods 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 3
- 102000012440 Acetylcholinesterase Human genes 0.000 claims 1
- 229940022698 acetylcholinesterase Drugs 0.000 claims 1
- 238000012360 testing method Methods 0.000 description 9
- 102100033639 Acetylcholinesterase Human genes 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 230000004075 alteration Effects 0.000 description 3
- 230000003851 biochemical process Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000012055 fruits and vegetables Nutrition 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 208000001490 Dengue Diseases 0.000 description 1
- 206010012310 Dengue fever Diseases 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010029350 Neurotoxicity Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 206010044221 Toxic encephalopathy Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000004657 carbamic acid derivatives Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 208000025729 dengue disease Diseases 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000006386 memory function Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000007135 neurotoxicity Effects 0.000 description 1
- 231100000228 neurotoxicity Toxicity 0.000 description 1
- 239000003987 organophosphate pesticide Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A pesticide detection device comprises an LED light source, a cuvette, a light sensor and a microcontroller. The LED light source can generate a light path with a certain wavelength, wherein the certain wavelength corresponds to at least one pesticide. The cuvette is located on the light path and has a container chamber to hold a solution containing the pesticide. The cuvette is typically transparent, allowing light on the light path to pass through. The light sensor is positioned on the light path and receives the light rays passing through the cuvette to generate a sensing signal. The microcontroller is electrically connected with the light sensor, receives the sensing signal and judges whether pesticide residues exist or not according to the sensing signal.
Description
Technical field
The present invention is about a kind of pesticide detection device; Especially in regard to a kind of optical agricultural chemical pick-up unit utilizing specific wavelength.
Background technology
Detecting Pesticide is divided into chemical method and biochemical process two kinds usually.Health unit control laboratory generally uses chemical method, takes 3 to 5 days, not only time-consuming and analytical instrument is expensive, bulky; And the biochemical genealogy of law utilizes Pepsin color reaction, the quick detection done with being usually applicable to limit, field and production and marketing, but still need spectral instrument to judge, and whether the total amount only surveying organic phosphate agent exceedes, and which kind of agricultural chemicals and content thereof cannot be learnt.Fruit and vegetable market is detected with biochemical process usually, and the fruit and vegetable of fuzzy area scope still can be auctioned away.Within 3-5 days, come out of the stove by chemical method assay afterwards, can only open if defective and penalize.In addition, in actual operation, from sampling, to announce result be often one after thoughtful two months to the examination of the agricultural chemicals of health unit, and problematic dish is already from market access consumption end.No matter be therefore that chemical method or biochemical process all cannot immediately and effectively confirm residues of pesticides, make dangerous vegetables and fruits be full of market, harm consumer health is to huge.
Cannot the problem of Rapid Detection for solving, develop the Pesticides Testing instrument utilizing optical principle gradually.Usually the luminophor of this optical agricultural chemical detector uses tungsten lamp or mercury lamp, and wavelength coverage is 350 to 700nm about.This traditional optical detector has the advantage of full spectrum function, can detect agricultural chemicals or other chemical compositions of various different spectrum.Precisely because price is high, bulky, part easily consumes and complicated operation, therefore always cannot universalness.
Therefore, need badly at present and want a kind of low price and simple and practical pesticide detection device, can for each vegetables and fruits path business even ordinary consumer immediately detect remains of pesticide, for control oneself and the health of other consumers is effectively checked on.
Summary of the invention
The present invention is about a kind of pesticide detection device, and the LED utilizing standing wave long, can effectively and carry out agricultural chemicals interpretation fast as light source, therefore the quite suitable exclusive instrument as Pesticides Testing.
According to one embodiment of the invention, a kind of pesticide detection device comprises LED light source, cuvette (cuvette), OPTICAL SENSORS and microcontroller.LED light source can produce the long light path of standing wave, and wherein this standing wave is long corresponds at least one agricultural chemicals.This cuvette is positioned on this light path, and has a vessel to hold the solution comprising this agricultural chemicals.Cuvette is generally transparent, allows the light on this light path to pass through.OPTICAL SENSORS is positioned on this light path, accepts the light by cuvette, produces sensing signal.Microcontroller is electrically connected this OPTICAL SENSORS, accepts the interpretation whether this sensing signal also has agricultural chemicals defective material according to this.
In one embodiment, this microcontroller is electrically connected this LED light source, with transfer control signal to LED light source.This pesticide detection device separately can comprise the analog-digital converter (analog-to-digitalconverter be connected between microcontroller and OPTICAL SENSORS; ADC), in order to this sensing signal is converted to digital signal, analysis interpretation is carried out for microcontroller.
In one embodiment, this light path be arranged between this LED light source and cuvette can comprise the first focus lamp, with focused ray in this cuvette.This light path be arranged between this cuvette and OPTICAL SENSORS can comprise the second focus lamp, with focused ray in this OPTICAL SENSORS.
In one embodiment, pesticide detection device can separately comprise a data input device, display screen, data storage device etc., to provide the convenience in use.
In one embodiment, this LED light source be wavelength 350 ~ 450nm determine wavelength light source.
Accompanying drawing explanation
Fig. 1 is the schematic appearance of the pesticide detection device of one embodiment of the invention.
Fig. 2 is the circuit box schematic diagram of the pesticide detection device of one embodiment of the invention.
Fig. 3 is the light path schematic diagram of the pesticide detection device of one embodiment of the invention.
Wherein, description of reference numerals is as follows:
10 pesticide detection devices
11 display screens
12 data input devices
13 keyboards
14 switch keys
15 power supply supply orifices
21LED light source
22 cuvettes
23 OPTICAL SENSORS
24 microcontrollers
25 analog-digital converters
26 data storage devices
27 batteries
28 clock generators
29 hummers
30 civil powers
31,32 focus lamps
Embodiment
Make by a definite date can have understanding clear and definite more in detail for the object of the present invention, effect and structural attitude, hereby lift preferred embodiment and coordinate accompanying drawing illustrate as after, only these illustrate to be only for the present invention is described, but not do any restriction to interest field of the present invention.
Fig. 1 is the pesticide detection device schematic diagram of one embodiment of the invention.The outward appearance of one pesticide detection device 10 as hand-held electronic instrument, volume is little, is convenient for carrying use.The surface of pesticide detection device 10 is provided with display screen 11 and data input device 12.Display screen 11 can the information such as display instrument operation, setting, testing process, testing result, data transmission, reads and use for user.Data input device 12 can comprise as keyboard 13 and switch key 14 etc.Keyboard 13 can key in related data and data, and switch key 14 can carry out switching between various pattern or other settings.In one embodiment, the side of pesticide detection device 10 is provided with power supply supply orifice 15, is connected to civil power by power lead, to provide power supply needed for pesticide detection device 10.Fig. 1 is only the outward appearance illustration figure of pesticide detection device 10 of the present invention, other similar outward appearances or structure, uses technology contents of the present invention still by the present invention is contained as long as same.
Fig. 2 is the circuit box schematic diagram of the pesticide detection device of one embodiment of the invention.Pesticide detection device 10 comprises display screen 11, data input device 12, LED light source 21, cuvette 22, OPTICAL SENSORS 23, microcontroller 24, analog-digital converter 25, data storage device 26, battery 27, clock generator 28 and hummer 29.Display screen 11 can be LCDs (LCD), and electrical connection microcontroller 24, in order to receive the display data and the control signal such as LCD screen brightness that microcontroller 24 transmits.LED light source 21 and OPTICAL SENSORS 23 are all electrically connected to microcontroller 24.Microcontroller 24 transfer control signal is to LED light source 21.In one embodiment, LED light source 21 adopt wavelength be 350 ~ 450nm determine wavelength light source, such as 412nm determines wavelength light source.In one embodiment, OPTICAL SENSORS 23 can be photodiodes (photodiode), can convert the light signal received to the anaiog signal such as voltage or electric current.Be provided with analog-digital converter 25 between OPTICAL SENSORS 23 and microcontroller 24, the anaiog signal in order to OPTICAL SENSORS 23 to be produced is converted to microcontroller 24 can the digital signal of interpretation and computing.Microcontroller 24 has the corresponding spectrum of various agricultural chemicals, the digital signal of reception and this spectrum can be compared, whether there to be the interpretation of residues of pesticides.In one embodiment, data input device 12 is connected to microcontroller 24, is available for users to input related data and carries out instrument setting.Pesticide detection device 10 can rely on battery 27 or connect two kinds of power supply methods of supplying such as civil power 30, and therefore microcontroller 24 connects battery 27 or is connected to civil power 30 by the power supply supply orifice 15 as Fig. 1.In fact, also can be designed to select separately a power supply.Data storage device 26 connects microcontroller 24, wherein can be provided with memory body and to give information memory function.Clock generator 28 provides detection time record at that time, to provide instant and the detection data of tool public credibility.Hummer 29 connects microcontroller 24, sends the attention sound in appropriate time.Such as: when the signal that microcontroller 24 receives OPTICAL SENSORS 23 completes detection, can the sound be sent, note to remind user; Or aural warning user is sent when being tested with residues of pesticides.In addition, pesticide detection device 10 of the present invention can be provided with USB joint or wireless module (not shown), or function of prining of can giving information in addition, or uses communication wafer further by data transmission to high in the clouds, to carry out data process and computing.
Fig. 3 is the light path schematic diagram of pesticide detection device 10 of the present invention.LED light source 21 send LED light via focus lamp 31 by burnt for light tool in cuvette 22.Cuvette 22 is generally transparent or semitransparent design, to allow that light passes through.This cuvette 22 has a vessel, can hold the solution comprising this agricultural chemicals.Light by another focus lamp 32 can be utilized after this cuvette 22 by light focusing in OPTICAL SENSORS 23, to improve the precision of sensing signal.The light path of pesticide detection device of the present invention on the same axis.
For the agricultural chemicals containing organophosphorus, Cleaning Principle of the present invention is described below.Organophosphorus thing is current most widely used agricultural chemicals in the world, not only for crop pest control, also can be used as environment medication, particularly the multiplying of anti-blocking dengue fever vector mosquito.Organophosphorus pesticide is easily decomposed in the environment, but has strong neurotoxicity, if having in sprinkling process accidentally, just may cause poisoning.The ultimate principle system of agricultural chemicals identification of the present invention utilizes ferment albumen to form the composition of cuvette solution, and will without agricultural chemicals person as a control group (normal medicament).Utilize the reaction of organophosphorus in Pepsin and agricultural chemicals in test group solution, when light is by after cuvette that sample sets solution (test medicament) be housed, color change is utilized to calculate light percent of pass, such as original light is 100%, if have 20% to be absorbed by the solution in cuvette, namely representative has 80% to pass through.And 100% light should be had to pass through without the normal medicament of agricultural chemicals.In brief, sensing signal is Pepsin solution without agricultural chemicals or the result that has the Pepsin solution of agricultural chemicals to compare, can judge whether residues of pesticides whereby.
Further it, usual solution to be measured comprises ferment albumen, related auxiliaries (such as photoghraphic coupler, by matter and damping fluid etc.) and determinand (as various agricultural chemicals) etc.Because ferment albumen is more unstable, this test first replaces agricultural chemicals to be measured with alcohol and carries out testing as a control group, and its measuring value is colourity, can be used as the index of penetrability, and result as shown in Table 1.The control group display of table one increases in time, and the value of chromatism of interval time (every 10 seconds) remains stable, that is colourity becomes arithmetic series to increase, and it shows instrument to be measured quite stable degree, can test.
[table]
| Second | Measuring value | Aberration |
| 10 | 0.271 | - |
| 20 | 0.353 | 0.082 |
| 30 | 0.436 | 0.083 |
| 40 | 0.519 | 0.083 |
| 50 | 0.601 | 0.082 |
| 60 | 0.683 | 0.082 |
Table two shows the first sample and uses the part agricultural chemicals containing Carbamate Class or organophosphorus Class, is that color method (acetylcholinesterase Acetylcholinesterase, AChE) judges residues of pesticides with AChE.The slope of measuring value represents the active situation of ferment.The larger expression enzyme activity of slope is stronger.Therefore the difference of comparison sample and control group slope can calculate the downtrod inhibiting rate of ferment.Inhibiting rate=[slope of (slope of the slope-sample sets measuring value of control group measuring value)/control group measuring value] × 100%.When the AChE inhibiting rate detected is less than default critical value (such as 40%), namely judge that agricultural chemicals is in acceptability limit, instrument can give a green light or blue lamp represents and upchecks.If AChE inhibiting rate is higher than this critical value, represent that agricultural chemicals exceeds standard, instrument will send out a warning, to warn.The inhibiting rate of table two shownschematically the first sample is about 78.8%, significantly more than the critical value 40% of the present embodiment setting, therefore differentiates there are residues of pesticides.
[table two]
| Second | Measuring value | Aberration |
| 10 | 0.160 | - |
| 20 | 0.177 | 0.018 |
| 30 | 0.195 | 0.018 |
| 40 | 0.213 | 0.018 |
| 50 | 0.231 | 0.018 |
| 60 | 0.249 | 0.018 |
Table three is the second Samples EXAMPLE, wherein detects that the inhibiting rate of sample is about 80%, and same judgement has residues of pesticides.
[table three]
| Second | Measuring value | Aberration |
| 10 | 0.145 | - |
| 20 | 0.163 | 0.017 |
| 30 | 0.179 | 0.016 |
| 40 | 0.196 | 0.017 |
| 50 | 0.213 | 0.017 |
| 60 | 0.230 | 0.017 |
The specific wavelength that pesticide detection device of the present invention collocation agricultural chemicals is corresponding, uses and simply determines the judgement effectively whether waveform LED light source can have residues of pesticides, not only precision is high, volume is little for it, easy to carry, and low price.In addition, the part coefficient of losses of pesticide detection device of the present invention is low, according to required, can carry out parameter and simply set.
The above, be only the bright and accompanying drawing of the Xiang Xi Said of specific embodiments of the invention, only the feature of the present invention is not limited thereto, all scopes of the present invention should be as the criterion with Claims scope, all embodiments of closing spirit change similar with it in the present patent application the scope of the claims, all should be contained in the category of the present invention, any those skilled in the art in the field of the invention, can think easily and change or modify and all can be encompassed in the scope of the claims of following this case.
Claims (13)
1. a pesticide detection device, comprising:
One LED light source, can produce the light path that standing wave is long, and wherein this standing wave is long corresponds at least one agricultural chemicals;
Cuvette, is positioned on this light path, has a vessel, can hold the solution comprising this agricultural chemicals, and allows the light on this light path to pass through;
OPTICAL SENSORS, is positioned on this light path, accepts the light by cuvette, produces sensing signal; And
Microcontroller, is electrically connected this OPTICAL SENSORS, accepts this sensing signal and carries out residues of pesticides interpretation according to this.
2. pesticide detection device according to claim 1, wherein this microcontroller is electrically connected this LED light source, with transfer control signal to LED light source.
3. pesticide detection device according to claim 1, wherein also comprises analog-digital converter, and it is electrically connected in microcontroller and OPTICAL SENSORS, in order to this sensing signal is converted to digital signal, analyzes for microcontroller.
4. pesticide detection device according to claim 1, this light path be wherein arranged between this LED light source and cuvette comprises the first focus lamp, with focused ray in this cuvette.
5. pesticide detection device according to claim 4, this light path be wherein arranged between this cuvette and OPTICAL SENSORS comprises the second focus lamp, with focused ray in this OPTICAL SENSORS.
6. pesticide detection device according to claim 1, it also comprises a data input device, is electrically connected in this microcontroller.
7. pesticide detection device according to claim 1, it also comprises a display screen, is electrically connected in this microcontroller.
8. pesticide detection device according to claim 1, it also comprises a data storage device, is electrically connected in this microcontroller.
9. pesticide detection device according to claim 1, it also comprises a battery, is electrically connected to this microcontroller.
10. pesticide detection device according to claim 1, wherein this LED light source be wavelength 350 ~ 450nm determine wavelength light source.
11. pesticide detection devices according to claim 1, its Pesticide Residues interpretation is control group without the Pepsin solution of agricultural chemicals and the result that has the sample sets of the Pepsin solution of agricultural chemicals to compare.
12. pesticide detection devices according to claim 11, it is color method that its Pesticide Residues interpretation comprises employing acetylcholinesterase.
13. pesticide detection devices according to claim 11, its Pesticide Residues interpretation according to the inhibiting rate of sample sets whether more than a critical value, wherein inhibiting rate=[slope of (slope of the slope-sample sets measuring value of control group measuring value)/control group measuring value] × 100%.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW102131594A TWI519773B (en) | 2013-09-03 | 2013-09-03 | Pesticide detection apparatus |
| TW102216490U TWM470254U (en) | 2013-09-03 | 2013-09-03 | Pesticide detection apparatus |
| TW102131594 | 2013-09-03 | ||
| TW102216490 | 2013-09-03 |
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|---|---|
| CN105181681A true CN105181681A (en) | 2015-12-23 |
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| CN201420375328.7U Active CN204154651U (en) | 2013-09-03 | 2014-07-08 | Pesticide detection device |
| CN201410322517.2A Pending CN105181681A (en) | 2013-09-03 | 2014-07-08 | Pesticide detection device |
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Cited By (3)
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|---|---|---|---|---|
| TWI620930B (en) * | 2016-11-18 | 2018-04-11 | 財團法人工業技術研究院 | Residual toxicant detection system and method thereof |
| CN108072617A (en) * | 2016-11-18 | 2018-05-25 | 财团法人工业技术研究院 | residual poison detection device |
| CN108072621A (en) * | 2016-11-18 | 2018-05-25 | 财团法人工业技术研究院 | Residual poison detection system and method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204154651U (en) * | 2013-09-03 | 2015-02-11 | 驷丞科技有限公司 | Pesticide detection device |
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| CN204154651U (en) | 2015-02-11 |
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Application publication date: 20151223 |