TWI568848B - 使用與CrylAb組合之DIG3殺蟲晶體蛋白管理歐洲玉米螟之抗性 - Google Patents
使用與CrylAb組合之DIG3殺蟲晶體蛋白管理歐洲玉米螟之抗性 Download PDFInfo
- Publication number
- TWI568848B TWI568848B TW101128023A TW101128023A TWI568848B TW I568848 B TWI568848 B TW I568848B TW 101128023 A TW101128023 A TW 101128023A TW 101128023 A TW101128023 A TW 101128023A TW I568848 B TWI568848 B TW I568848B
- Authority
- TW
- Taiwan
- Prior art keywords
- protein
- cry1ab
- toxin
- plant
- plants
- Prior art date
Links
- ZHVOBYWXERUHMN-KVJKMEBSSA-N 3-[(3s,5r,8r,9s,10s,13s,14s,17s)-10,13-dimethyl-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2h-furan-5-one Chemical compound O([C@@H]1C[C@H]2CC[C@@H]3[C@@H]([C@]2(CC1)C)CC[C@]1([C@H]3CC[C@@H]1C=1COC(=O)C=1)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZHVOBYWXERUHMN-KVJKMEBSSA-N 0.000 title claims description 45
- 230000000749 insecticidal effect Effects 0.000 title claims description 39
- 241001147398 Ostrinia nubilalis Species 0.000 title claims description 10
- 101710151559 Crystal protein Proteins 0.000 title description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 186
- 102000004169 proteins and genes Human genes 0.000 claims description 117
- 241000196324 Embryophyta Species 0.000 claims description 76
- 238000000034 method Methods 0.000 claims description 49
- 240000008042 Zea mays Species 0.000 claims description 28
- 230000027455 binding Effects 0.000 claims description 28
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 27
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 26
- 235000005822 corn Nutrition 0.000 claims description 26
- 241000238631 Hexapoda Species 0.000 claims description 25
- 238000012546 transfer Methods 0.000 claims description 17
- 229920000742 Cotton Polymers 0.000 claims description 6
- 108091033319 polynucleotide Proteins 0.000 claims description 5
- 102000040430 polynucleotide Human genes 0.000 claims description 5
- 239000002157 polynucleotide Substances 0.000 claims description 5
- 241001057636 Dracaena deremensis Species 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 238000011161 development Methods 0.000 claims description 2
- 230000002463 transducing effect Effects 0.000 claims 2
- 241000219146 Gossypium Species 0.000 claims 1
- 108700012359 toxins Proteins 0.000 description 117
- 239000003053 toxin Substances 0.000 description 109
- 231100000765 toxin Toxicity 0.000 description 109
- 235000018102 proteins Nutrition 0.000 description 103
- 210000004027 cell Anatomy 0.000 description 39
- 241000724266 Broad bean mottle virus Species 0.000 description 21
- 241000607479 Yersinia pestis Species 0.000 description 21
- 239000002917 insecticide Substances 0.000 description 16
- 238000006467 substitution reaction Methods 0.000 description 15
- 244000005700 microbiome Species 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 12
- 239000000203 mixture Substances 0.000 description 12
- 210000002706 plastid Anatomy 0.000 description 12
- 108020003175 receptors Proteins 0.000 description 12
- 102000005962 receptors Human genes 0.000 description 12
- 239000002243 precursor Substances 0.000 description 11
- 239000000523 sample Substances 0.000 description 11
- 239000013598 vector Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 description 10
- 101150102464 Cry1 gene Proteins 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 9
- 230000009471 action Effects 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 238000009472 formulation Methods 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 238000011282 treatment Methods 0.000 description 9
- 206010034133 Pathogen resistance Diseases 0.000 description 7
- 230000004071 biological effect Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000002285 radioactive effect Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000255777 Lepidoptera Species 0.000 description 5
- 239000011324 bead Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 239000002953 phosphate buffered saline Substances 0.000 description 5
- 230000009261 transgenic effect Effects 0.000 description 5
- 241000346285 Ostrinia furnacalis Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 4
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 4
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000002596 immunotoxin Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000002287 radioligand Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 230000008685 targeting Effects 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 3
- 241000193388 Bacillus thuringiensis Species 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000001412 amines Chemical group 0.000 description 3
- 229940097012 bacillus thuringiensis Drugs 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000011539 homogenization buffer Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000003094 microcapsule Substances 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 231100000654 protein toxin Toxicity 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 239000003440 toxic substance Substances 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 2
- 241000588986 Alcaligenes Species 0.000 description 2
- 241000223651 Aureobasidium Species 0.000 description 2
- 241000589151 Azotobacter Species 0.000 description 2
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 241000879145 Diatraea grandiosella Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 108010042653 IgA receptor Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000235649 Kluyveromyces Species 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 102100034014 Prolyl 3-hydroxylase 3 Human genes 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000589180 Rhizobium Species 0.000 description 2
- 241000190932 Rhodopseudomonas Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 241000256251 Spodoptera frugiperda Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000012148 binding buffer Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000012669 liquid formulation Substances 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 238000000329 molecular dynamics simulation Methods 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 230000000361 pesticidal effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000003322 phosphorimaging Methods 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 244000235858 Acetobacter xylinum Species 0.000 description 1
- 235000002837 Acetobacter xylinum Nutrition 0.000 description 1
- 238000008940 Alkaline Phosphatase assay kit Methods 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 229920002799 BoPET Polymers 0.000 description 1
- 239000011547 Bouin solution Substances 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 239000008001 CAPS buffer Substances 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 241000122106 Diatraea saccharalis Species 0.000 description 1
- 241000353522 Earias insulana Species 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000190714 Gymnosporangium clavipes Species 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 108010060231 Insect Proteins Proteins 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000005041 Mylar™ Substances 0.000 description 1
- 241001443590 Naganishia albida Species 0.000 description 1
- 241000033319 Naganishia diffluens Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001147397 Ostrinia Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000222051 Papiliotrema laurentii Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000269423 Rhinella marina Species 0.000 description 1
- 241000223252 Rhodotorula Species 0.000 description 1
- 241000223253 Rhodotorula glutinis Species 0.000 description 1
- 241000223254 Rhodotorula mucilaginosa Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241001479507 Senecio odorus Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000222068 Sporobolomyces <Sporidiobolaceae> Species 0.000 description 1
- 241000123675 Sporobolomyces roseus Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241001501944 Suricata Species 0.000 description 1
- 244000288561 Torulaspora delbrueckii Species 0.000 description 1
- 235000014681 Torulaspora delbrueckii Nutrition 0.000 description 1
- 241001495125 Torulaspora pretoriensis Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 241000589636 Xanthomonas campestris Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229960005475 antiinfective agent Drugs 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- OCBHHZMJRVXXQK-UHFFFAOYSA-M benzyl-dimethyl-tetradecylazanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 OCBHHZMJRVXXQK-UHFFFAOYSA-M 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229960001927 cetylpyridinium chloride Drugs 0.000 description 1
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 102000054767 gene variant Human genes 0.000 description 1
- 108010067216 glycyl-glycyl-glycine Proteins 0.000 description 1
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 230000002140 halogenating effect Effects 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000003750 lower gastrointestinal tract Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 231100001225 mammalian toxicity Toxicity 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229910052615 phyllosilicate Inorganic materials 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000012536 storage buffer Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Pest Control & Pesticides (AREA)
- Physics & Mathematics (AREA)
- Insects & Arthropods (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161515553P | 2011-08-05 | 2011-08-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201311892A TW201311892A (zh) | 2013-03-16 |
| TWI568848B true TWI568848B (zh) | 2017-02-01 |
Family
ID=47627858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW101128023A TWI568848B (zh) | 2011-08-05 | 2012-08-03 | 使用與CrylAb組合之DIG3殺蟲晶體蛋白管理歐洲玉米螟之抗性 |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20130036520A1 (enExample) |
| EP (1) | EP2739133A4 (enExample) |
| JP (1) | JP2014525748A (enExample) |
| KR (1) | KR20140056323A (enExample) |
| CN (1) | CN103841821A (enExample) |
| AR (1) | AR087456A1 (enExample) |
| AU (1) | AU2012294678B2 (enExample) |
| CA (1) | CA2843642A1 (enExample) |
| CL (1) | CL2014000282A1 (enExample) |
| CO (1) | CO6900119A2 (enExample) |
| MX (1) | MX2014001456A (enExample) |
| PH (1) | PH12014500291A1 (enExample) |
| RU (1) | RU2624031C2 (enExample) |
| TW (1) | TWI568848B (enExample) |
| UY (1) | UY34240A (enExample) |
| WO (1) | WO2013022743A1 (enExample) |
| ZA (1) | ZA201401514B (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5873005B2 (ja) * | 2009-04-17 | 2016-03-01 | ダウ アグロサイエンシィズ エルエルシー | Dig−3殺虫性cryトキシン |
| TW201615095A (zh) | 2014-10-31 | 2016-05-01 | 陶氏農業科學公司 | Dig-303殺蟲cry毒素 |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CN106591352B (zh) * | 2016-11-21 | 2020-05-05 | 北京大北农科技集团股份有限公司 | 杀虫蛋白组合及其管理昆虫抗性的方法 |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CN112438198A (zh) * | 2019-08-30 | 2021-03-05 | 中国农业大学 | 利用杂交不亲和基因在制备抗虫转基因玉米庇护所中的应用 |
| CN111606984B (zh) * | 2020-05-19 | 2021-08-06 | 隆平生物技术(海南)有限公司 | 一种植物抗虫蛋白及其编码基因和应用 |
| CN118742647A (zh) * | 2022-02-15 | 2024-10-01 | 富优基尼以色列股份有限公司 | 对植物保护有用的苏云金芽孢杆菌杀虫蛋白(Bt PP)组合 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070006340A1 (en) * | 2004-03-05 | 2007-01-04 | Lang Bruce A | Combinations of Cry1Ab and Cry1Fa as an insect resistance management tool |
| US20100269223A1 (en) * | 2009-04-17 | 2010-10-21 | Dow Agrosciences Llc | Dig-3 insecticidal cry toxins |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA48104C2 (uk) * | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
| EP0589110A1 (en) * | 1992-08-19 | 1994-03-30 | Plant Genetic Systems N.V. | Control of ostrinia |
| GB9318207D0 (en) * | 1993-09-02 | 1993-10-20 | Sandoz Ltd | Improvements in or relating to organic compounds |
| WO2008080166A2 (en) * | 2006-12-22 | 2008-07-03 | Pioneer Hi-Bred International, Inc. | Resistance management strategies for transgenic crops |
| UA111814C2 (uk) * | 2009-12-16 | 2016-06-24 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | ТРАНСГЕННА РОСЛИНА, ЯКА МІСТИТЬ Cry1Ab ТА Cry2Aa, ДЛЯ РЕГУЛЮВАННЯ ЄВРОПЕЙСЬКОГО КУКУРУДЗЯНОГО МЕТЕЛИКА І СПОСОБИ БОРОТЬБИ ЗІ СТІЙКІСТЮ КОМАХ |
| WO2011084631A1 (en) * | 2009-12-16 | 2011-07-14 | Dow Agrosciences Llc | Use of cry1ab in combination with cry1be for management of resistant insects |
| KR101841296B1 (ko) * | 2009-12-16 | 2018-03-22 | 다우 아그로사이언시즈 엘엘씨 | 곤충 내성 관리를 위한, cry1be와 조합된 cry1da의 용도 |
-
2012
- 2012-08-03 JP JP2014524101A patent/JP2014525748A/ja active Pending
- 2012-08-03 WO PCT/US2012/049491 patent/WO2013022743A1/en not_active Ceased
- 2012-08-03 TW TW101128023A patent/TWI568848B/zh not_active IP Right Cessation
- 2012-08-03 PH PH1/2014/500291A patent/PH12014500291A1/en unknown
- 2012-08-03 RU RU2014108317A patent/RU2624031C2/ru not_active IP Right Cessation
- 2012-08-03 MX MX2014001456A patent/MX2014001456A/es unknown
- 2012-08-03 AU AU2012294678A patent/AU2012294678B2/en not_active Ceased
- 2012-08-03 AR ARP120102851A patent/AR087456A1/es unknown
- 2012-08-03 UY UY0001034240A patent/UY34240A/es not_active Application Discontinuation
- 2012-08-03 KR KR1020147005928A patent/KR20140056323A/ko not_active Ceased
- 2012-08-03 CN CN201280049141.1A patent/CN103841821A/zh active Pending
- 2012-08-03 CA CA2843642A patent/CA2843642A1/en not_active Abandoned
- 2012-08-03 EP EP12822625.5A patent/EP2739133A4/en not_active Withdrawn
- 2012-08-03 US US13/566,277 patent/US20130036520A1/en not_active Abandoned
-
2014
- 2014-02-04 CL CL2014000282A patent/CL2014000282A1/es unknown
- 2014-02-27 ZA ZA2014/01514A patent/ZA201401514B/en unknown
- 2014-03-05 CO CO14047170A patent/CO6900119A2/es unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070006340A1 (en) * | 2004-03-05 | 2007-01-04 | Lang Bruce A | Combinations of Cry1Ab and Cry1Fa as an insect resistance management tool |
| US20100269223A1 (en) * | 2009-04-17 | 2010-10-21 | Dow Agrosciences Llc | Dig-3 insecticidal cry toxins |
Non-Patent Citations (1)
| Title |
|---|
| Bravo A et al., "How to cope with insect resistance to Bt toxins?", TRENDS IN BIOTECHNOLOGY, Vol.26, No.10, Pages 573–579, 2008/08/14 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201311892A (zh) | 2013-03-16 |
| MX2014001456A (es) | 2014-02-27 |
| WO2013022743A1 (en) | 2013-02-14 |
| AR087456A1 (es) | 2014-03-26 |
| RU2014108317A (ru) | 2015-09-10 |
| CL2014000282A1 (es) | 2014-07-11 |
| US20130036520A1 (en) | 2013-02-07 |
| EP2739133A1 (en) | 2014-06-11 |
| KR20140056323A (ko) | 2014-05-09 |
| PH12014500291A1 (en) | 2020-12-18 |
| EP2739133A4 (en) | 2014-12-17 |
| RU2624031C2 (ru) | 2017-06-30 |
| AU2012294678B2 (en) | 2017-10-05 |
| NZ621811A (en) | 2016-03-31 |
| UY34240A (es) | 2013-04-05 |
| JP2014525748A (ja) | 2014-10-02 |
| AU2012294678A1 (en) | 2014-03-20 |
| CA2843642A1 (en) | 2013-02-14 |
| CN103841821A (zh) | 2014-06-04 |
| ZA201401514B (en) | 2015-10-28 |
| CO6900119A2 (es) | 2014-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102753695B (zh) | 组合使用Cry1Ab 与Cry1Be 用于抗性昆虫的管理 | |
| JP5969921B2 (ja) | 抵抗性昆虫の対応のためのCry1Beと組み合わせたCry1Daの使用 | |
| RU2569108C2 (ru) | ПРИМЕНЕНИЕ Cry1Da В СОЧЕТАНИИ С Cry1Ca ДЛЯ УПРАВЛЕНИЯ УСТОЙЧИВЫМИ НАСЕКОМЫМИ | |
| CN102753013B (zh) | 组合使用Vip3Ab与Cry1Ca用于抗性昆虫的管理 | |
| TWI568848B (zh) | 使用與CrylAb組合之DIG3殺蟲晶體蛋白管理歐洲玉米螟之抗性 | |
| WO2011075587A1 (en) | Combined use of cry1da and cry1fa proteins for insect resistance management | |
| EP2513314A1 (en) | Combined use of cry1ca and cry1ab proteins for insect resistance management | |
| CN104902744A (zh) | Cry1Ea在组合中用于管理抗性秋粘虫昆虫的用途 | |
| US10119149B2 (en) | Use of DIG3 insecticidal crystal protein in combination with cry1Ab for management of resistance in european cornborer | |
| JP5913124B2 (ja) | サトウキビでのCry抵抗性のシュガーケーンボーラーの防除および昆虫抵抗性管理のためのCRY1FaおよびCRY1Abタンパク質の併用 | |
| RU2575084C2 (ru) | ПРИМЕНЕНИЕ Vip3Ab В СОЧЕТАНИИ С Cry1Ca ДЛЯ УПРАВЛЕНИЯ УСТОЙЧИВЫМИ НАСЕКОМЫМИ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Annulment or lapse of patent due to non-payment of fees |