TWI535448B - A composition of decreasing the accumulation of fat and use thereof - Google Patents

Description

Composition for inhibiting fat accumulation and use thereof

The present invention provides a composition having reduced body weight gain and reduced fat accumulation, the composition comprising Bacillus subtilis and Forskohlii extract.

Obesity is undoubtedly the most important health issue in modern society and the first in modern civilization. There are many factors that cause obesity, including a decrease in physical activity, malnutrition, excessive intake of starch and sugar, or excessive intake of oil. Among them, excessive intake of oil is one of the most important factors, especially now because people are eating. The change of type and the increase of food consumption lead to excessive intake of unnecessary oils. Even those who choose food carefully can not avoid the existence of invisible oils in various fresh foods and processed foods, plus busy working life and returning to work. After the home is in the most comfortable and relaxed state, this state will cause a large accumulation of body fat in the long run, making weight loss more difficult and putting health in a dangerous situation.

According to research, about 75-80% of the civilized social population will be overweight or obese in their lifetime. In addition to causing defects in appearance, and forming psychological and social obstacles and further affecting work ability, obesity is also prone to many symptoms, including edema, cardiac hypertrophy, fatty liver, bile and urinary calculi, and musculoskeletal pain. Disease, gynecological breast Or uterine tumor, hyperuricemia (gout), hyperlipidemia, angina, diabetes, high blood pressure, stroke, etc. According to the latest statistics of the Department of Health, the proportion of adult males who are overweight or obese in China has reached 51%, and women have reached 36%. The ratio of overweight or obesity in primary school children in Yuguo is 29% for boys and 21.0% for girls. The ratio of overweight and obesity continues to rise. In addition, among the top ten causes of death among Chinese people, malignant tumors, heart disease, cerebrovascular disease, diabetes, chronic lower respiratory disease, hypertension, chronic liver disease, liver cirrhosis, and chronic kidney disease The cause of death is related to obesity, so maintaining a healthy weight is the goal of joint efforts.

At present, the weight-loss methods are dazzling and ridiculous, and they all belong to unhealthy methods of weight loss, and regular exercise and change of diet are the most important methods. However, dieters often lack patience, lack of exercise and resistance to food are the biggest problems they face, so weight loss products are the best choice for dieters. Common weight loss products, such as the phenyl propanolamine approved by the Department of Health, can promote the body's metabolic rate, increase calorie consumption, reduce appetite, etc., and can see the effect of weight loss in a short period of time, but at the same time with heart palpitations Side effects such as vomiting, insomnia, and severe anorexia can cause the user to lose weight, but they also lose health.

Therefore, providing a healthy way to lose weight is an urgent task. The average person loses weight and only focuses on reducing "heavy" rather than "fat". That is to say, the body fat ratio does not necessarily change. However, lowering the body's triglyceride content means that the fat is actually reduced to fat. Improve your body's metabolism and make it easier to achieve your weight loss goals.

In order to solve the above problems, the present invention provides a composition of a plant extract and Bacillus subtilis, which can effectively achieve the purpose of healthy weight loss for a user.

The present invention provides a composition for inhibiting fat accumulation, which comprises an extract of Coleus formosana and Bacillus subtilis BS-139, which is a strain deposited in the No. BCRC 910457 of the Institute of Food Industry Development.

In an embodiment of the invention, the content of the extract of the genus Corydalis is about 0.01 wt% to 0.05 wt%, and the content of the B. subtilis BS 139 is about 0.02 wt% to 0.08 wt%.

In one embodiment of the invention, the composition is effective to reduce the body weight of an animal or a human.

In one embodiment of the invention, the composition is effective to reduce triglycerides in the blood of animals or humans.

In one embodiment of the invention, the composition reduces the uptake of calories in animal or human food.

In an embodiment of the invention, the composition is applicable to pharmaceuticals, health foods, health foods, foods and feeds.

The invention further provides a use of a composition of a plant extract for the preparation of a medicament for treating obesity, wherein the combination of the extract comprises: an effective dose of the extract of Coleus forsythiae, the content is 0.01 wt% to 0.05 wt%; The dose of Bacillus subtilis BS-139, which is stored in the Bioresource Conservation and Research Center of the Republic of China Food Industry Development Institute, is the strain of BCRC 910457, and the content is 0.02wt%~0.08wt%.

Figure 1 shows the change in body weight over days in female rats fed different diets.

Figure 2 shows the changes in weekly weight gain of female rats fed different diets.

Figure 3 shows the changes in feed intake per week for female rats fed different diets.

Figure 4 shows the results of feed efficiency of female rats fed different feeds.

Laboratory animals and breeding environment

The experimental animals used a five-week-old Wistar female rat, a total of 18 rats, each weighing about 160 grams, and were housed in a rodent house of the Institute of Animal Science and Technology of the Institute of Agricultural Science and Technology. The rodent chamber temperature was controlled at about 23 ° C (20-26 ° C) and provided with a 12-hour light photoperiod condition. The experimental rats were housed in a cage that was autoclaved and the drinking water was supplied in an autoclaved water bottle.

Establishment of Wistar female rats of different feed groups

The experimental rats were reared in two cages, and the animal number and the cage number were marked, and the test was carried out for six weeks after the 7-day acclimation period. Rats were randomly assigned to the control group, the refueling group, or the refueling and drug group, with 6 in each group, for a total of 3 cages. The control group was fed a special granular feed (Lab Diet® 5001 Rodent Diet) from imported rats. The refueling group is fed with high-fat feed supplemented with 10% salad oil. The high-fat feed production is pulverized with imported granular feed for rats, and then 10% salad oil is added to form a block, and to avoid the problem of oil oxidation, high The fat feed began to be prepared two days before feeding. The refueling and drug groups were also fed a high-fat diet, and Bacillus subtilis and Forskohlii extract were added. The main nutrients of the feed are listed in Table 1, where the data is an average.

Test analysis

Rats were weighed at the beginning of the trial (ie day 1) and weekly, and the weekly feed consumption of each rat was recorded. Clinical observations were performed daily during the trial to document whether the animals had clinical signs or death. At the end of the experiment (ie day 42), blood was collected from the tail vein and glucose, triglyceride, aspartate transaminase was measured by a blood biochemical analyzer (Kodak Ektachem DT-II System, Rochester, NY, USA). (AST) and alanine transaminase (ALT) content. Subsequently, the rats were sacrificed for gross pathological observation and the tissue weight was recorded.

Example 1: Feed and energy intake

Please refer to Table 2, during the test period, the feed intake of each group changed slightly, but the difference did not reach statistical significance (P>0.05), and the value of Table 2 was an average value.

Compared with the control group for feeding special feed, the refueling group was fed with high-fat feed with 10% salad oil. The feed intake in the first week was 8.6% lower than that of the control group, and the second week was decreased by 27.6%. The intake was higher than the control group and increased by 7-19% between the third and sixth weeks. Feed intake in the first and second weeks of the refueling group appeared to be reduced by the effect of oil addition. However, after two weeks of adaptation, the feed intake for the next four weeks was higher than that of the control group. During the whole experiment period, the total feed intake of the refueling group was similar to that of the control group, but the total energy intake was 15.6% higher than that of the control group, which was consistent with the purpose of increasing the energy intake of rats by applying high fat diet.

The refueling and drug groups were fed with high-fat diet, and then B. subtilis BS-139 and Coleus forsythiae extract were added. The change in intake was not consistent with the refueling group. The feed intake in the first week of refueling and drug group was 17.2% higher than that of the control group and 28.3% higher in the refueling group. The feed intake in the second week was similar to that in the refueling group, but was 27.6% lower than that in the control group. At 28.4% higher, the feed intake in the fourth and sixth weeks was 7% lower than that in the control group. The combination of Bacillus subtilis and Coleus formosanus extraction appears to have a situation in which the rats are prevented from ingesting the feed after one week (i.e., the second, fourth, and sixth weeks). During the whole experiment period, the total feed intake of the refueling and drug groups was similar to that of the control group, while the total energy intake was 13% higher than the control group, but 2.6% lower than the refueling group.

Example 2: Growth curve and weight change

Please refer to Figure 1. At the beginning of the experiment, the average body weight of each group was similar, about 177 grams. As the number of days increased, the body weight of each group increased. Among them, the increased body weight of the control group gradually slowed down with the increase of the number of days, and the growth curve of the fueling group was different from that of the control group. It is worth noting that the weight of the refueling and drug groups was lower than that of the control group and the refueling group on the 21st day, and the gap between the control group and the refueling group also increased with time, and the gap was not increased in an equidistant manner.

Please refer to Table 3, where the data in Table 3 is an average value, and the different superscripts in the same column indicate significant differences. The P values of a and b are less than 0.05, the P values of a and c are less than 0.01, and the P values of a and d are less than 0.001. .

The control group gained more than 22 g in the first and second weeks, 16.5 g in the third and fourth weeks, 7.7 g in the fifth week, and only 3.2 g in the sixth week. In the first week, the weight gain of the refueling group was only 8g, which was significantly lower than that of the control group (P<0.05). On the contrary, the weight gain in the second week of the test was significantly higher than that of the control group, reaching 42.3g (P<0.001), followed by 3 -6 weeks, there was no difference in weight gain between the two groups. The first week of weight gain in the refueling and drug groups did not differ from the other two groups, but the second week was significantly higher than the control group, reaching 35.2 g (P<0.01). At the third week, the refueling and drug groups only gained 5.2 g. Significantly lower than the control group and the refueling group (P<0.01), the subsequent 4-6 weeks weight gain was no different from the other two groups, but both were lower than the other two groups. During the test period, the total weight gain of the control group and the refueling group were 90.2 g and 98.5 g, respectively. In contrast, the total weight gain of the refueling and drug groups was only 74.8 g, which was significantly lower than the other two groups (P<0.05). The drug group began its weight curve from week 3 Below the control and refueling groups, it was demonstrated that feeding the feed containing the composition of B. subtilis and Coleus formosan extraction helped to lose weight.

Please refer to Figures 2 and 3. Rats fed high-fat diets may reduce their intake due to adaptation problems and further reduce weight gain (first week). However, the feed intake in the first week of refueling and drug group was higher than that of the control group by 17%, but the weight gain was 40% lower, indicating that the addition of Bacillus subtilis and Coleus forsythiae extract composition seems to have the effect of controlling weight gain. A similar situation was observed in the third, fourth, fifth, and sixth weeks, except for the second week. Compared with the control group, the third week of refueling and drug group intake was 3.8% higher, but the weight gain was 68.5% lower. In the fifth week, the test group intake was 28.4% higher, but the weight gain was 38.7% lower. Compared with the refueling group, the intake of the test group in the third week was 8.6% lower, but the weight gain was 74.3% lower. In the fifth week, the intake of the test group was 7.4% higher, but the weight gain was 39.7% lower. The results showed that the feed intake of the refueling and the drug group increased, but the body weight did not increase or decrease compared with the control group and the refueling group. It was proved that the feed of the composition containing the extract of Bacillus subtilis and Coleus formosanus can effectively control the increase of body weight.

Example 3: Tissue weight

During the test, all animals were healthy, no deaths, and no clinical symptoms were observed. Please refer to Table 4, where the data in Table 4 is an average value, and the different superscripts on the same line indicate significant differences. The P values of a and b are less than 0.05 and the P values of a and c are less than 0.01. After the animals were sacrificed, there was no difference in heart and kidney weight between the groups. However, the abdominal fat weight of the refueling group was 58.5% higher than that of the control group (P<0.05). The refueling and the drug group were also fed with high-fat diet, but the abdominal fat weight was 44.5% lower than that of the refueling group (P<0.01). 12.1% lower (P>0.05). In the refueling and drug group, the combination of Bacillus subtilis and Coleus formosanus extract inhibited the accumulation of abdominal fat in rats under similar energy intake conditions, and was significantly less than the control group and the refueling group. The composition of the invention is effective in reducing fat absorption and reducing fat accumulation.

Example 4: Blood chemistry changes

Please refer to Table 5, where the data in Table 5 is an average value, and the different superscripts on the same line indicate significant differences, and the P values of a and c are less than 0.01. Compared with the control group, the high-fat diet in the refueling group reduced the blood glucose concentration (P<0.01), but slightly increased the triglyceride concentration (16.5%). The glucose concentration in the refueling and drug groups was also lower than that in the control group (P<0.01), but the increase in triglyceride (5.5%) was 11% lower than that in the refueling group. It can be seen that when the foods and fats eaten are more, the combination of Bacillus subtilis and Coleus formosan extract can reduce triglyceride, and its mechanism may be independent of glucose production, but related to fat metabolism and synthesis. .

In addition, aspartic acid transaminase content and alanine transaminase content are clinically used to assess the degree of hepatocyte damage and acute and chronic classification of liver disease, and are also an important indicator of the effectiveness of liver disease treatment. Alanine transaminase is abundantly present in the liver, while aspartame Acid transaminase is found in the heart and muscles. When cells in these areas are damaged, especially liver cells, the activity of aspartate transaminase and alanine transaminase in serum is significantly increased. In this example, it can be seen that although the content of aspartic acid transaminase in the refueling group is lower than that of the control group, it does not affect high-density cholesterol and alanine transaminase; however, the asparagine transaminase content in the refueling group is slightly increased. However, the test group additions reduced the aspartate transaminase, but the difference between the groups was not significant.

Example 5: Feed efficiency

Please refer to Figure 4 to calculate the efficiency of the conversion of feed into body weight by dividing the total weight gain by the total feed intake. The control group is similar to the fueling group data. The feed efficiency of the refueling and drug groups was nearly 19% lower than that of the other two groups, indicating that rats fed B. subtilis BS-139 and Coleus forsythiae extracts required 19% more feed to achieve similar weight gain. Therefore, the extracting composition of the present invention containing Bacillus subtilis BS-139 and Coleus formosana has the effect of reducing nutrient absorption of the feed and converting into a body composition.

According to the experiment of the present invention, Wistar female rats ingesting high fat diets can increase body weight and abdominal fat accumulation, and the extract composition of Bacillus subtilis BS-139 and Coleus forsythia can control the weight gain induced by high fat diet ( 24.1%), and inhibited fat metabolism (triglyceride) and abdominal fat mass (44.5%), the magnitude of the decrease was greater than the increase in body weight and abdominal fat in the normal diet group, and the high fat was fed after six weeks. The feed and the rats to which the extract composition was added did not have any clinical symptoms (referred to as severe side effects) which were observable by the naked eye. It is apparent that the composition of the present invention inhibits weight gain, reduces fat absorption, and reduces fat accumulation without changing the appetite of the user, and has no side effects, and controls the body weight in a healthy manner.

【Biomaterial Storage】

Domestic registration information [please note according to the registration authority, date, number order]

Food Industry Research Institute

June 27, 103, Republic of China

Deposit number BCRC 910457

Claims (3)

A composition for inhibiting fat accumulation, comprising: about 0.01 wt% to 0.05 wt% of a extract of Coleus forsythia; and about 0.02 wt% to 0.08 wt% of B. subtilis BS139, the Bacillus subtilis is preserved in China The Bioresource Conservation and Research Center of the Research Institute of Food Industry Development of the Republic of China registers the strain number BCRC 910457, wherein the composition can reduce the weight gain of animals or humans by 20% to 40%, and the composition can effectively reduce the abdominal fat weight of animals or humans. %~80%, the composition can effectively reduce the triglyceride in animal or human blood by 5%~12%, and the composition can reduce the caloric intake of animal or human food by 15%~25%. The composition of claim 1 is applicable to pharmaceuticals, health foods, health foods, foods and feeds. A use of a composition of a plant extract for the preparation of a pharmaceutical composition for treating obesity, wherein the combination of the plant extract comprises: a Coleus forsythiae extract having a content of 0.01% by weight to 0.05% by weight; and a subtilis Bacillus sp. BS139, which is a strain stored in the Bioresource Conservation and Research Center of the Republic of China Food Industry Development Institute, registered under the number BCRC 910457, with a content of 0.02 wt% to 0.08 wt%, wherein the composition can reduce the increase of animals or humans. Weighing 20%~40%, the composition can effectively reduce the abdominal fat weight of animals or humans by 10%~80%, and the composition can effectively reduce the triglyceride in animal or human blood by 5%~12%, the composition It can reduce the caloric intake of animal or human food by 15%~25%.