TWI535448B - A composition of decreasing the accumulation of fat and use thereof - Google Patents
A composition of decreasing the accumulation of fat and use thereof Download PDFInfo
- Publication number
- TWI535448B TWI535448B TW103142403A TW103142403A TWI535448B TW I535448 B TWI535448 B TW I535448B TW 103142403 A TW103142403 A TW 103142403A TW 103142403 A TW103142403 A TW 103142403A TW I535448 B TWI535448 B TW I535448B
- Authority
- TW
- Taiwan
- Prior art keywords
- composition
- weight
- group
- refueling
- fat
- Prior art date
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Description
本發明提供一種具減少體重增加和降低脂肪累積的組合物,該組合物包含枯草桿菌BS-139(Bacillus subtilis)及毛喉鞘蕊花萃取(Forskohlii extract)。 The present invention provides a composition having reduced body weight gain and reduced fat accumulation, the composition comprising Bacillus subtilis and Forskohlii extract.
肥胖無疑是現代社會中最重要的健康課題,也成為現代文明病之首。造成肥胖的因素有很多種,包括身體活動量的減少、營養失調、澱粉及糖類過度攝取或是油脂的過度攝取等等,其中過度攝取油脂為最主要的因素之一,尤其是現在人因為飲食型態的改變、外食的增加,導致攝取過多不必要的油脂,即使是慎選食物者,也無法避免存在各種生鮮食品與加工食品中的隱形油脂,再加上忙碌的上班生活,且下班回家後便處於最舒適且放鬆的狀態,此狀態長期下來將造成體脂肪大量的累積,使減肥更加困難,並使健康置於危險狀況。 Obesity is undoubtedly the most important health issue in modern society and the first in modern civilization. There are many factors that cause obesity, including a decrease in physical activity, malnutrition, excessive intake of starch and sugar, or excessive intake of oil. Among them, excessive intake of oil is one of the most important factors, especially now because people are eating. The change of type and the increase of food consumption lead to excessive intake of unnecessary oils. Even those who choose food carefully can not avoid the existence of invisible oils in various fresh foods and processed foods, plus busy working life and returning to work. After the home is in the most comfortable and relaxed state, this state will cause a large accumulation of body fat in the long run, making weight loss more difficult and putting health in a dangerous situation.
根據研究指出,在文明社會人口中約有75-80%的人其一生會有過重或是肥胖的現象。肥胖除了造成外表的缺憾,並形成心理與社交的障礙且進一步影響工作能力之外,在生理上也容易形成許多病徵,包括水腫、心臟肥大、脂肪肝、膽肝泌尿系結石、肌肉骨骼諸痛症、婦科乳房 或子宮腫瘤、高尿酸症(痛風)、高血脂症、狹心症、糖尿病、高血壓、中風等。根據衛生署的最新統計,我國成年男性體重過重或肥胖者已達51%,女性亦達36%,於國小學童體重過重或肥胖之比率,男童為29%、女童為21.0%,可見國人過重及肥胖之比率持續上昇中,另外國人十大死亡原因中,惡性腫瘤、心臟疾病、腦血管疾病、糖尿病、慢性下呼吸道疾病、高血壓、慢性肝病及肝硬化、慢性腎臟病等8項死因皆與肥胖有關,因此維持健康體重成為共同努力的目標。 According to research, about 75-80% of the civilized social population will be overweight or obese in their lifetime. In addition to causing defects in appearance, and forming psychological and social obstacles and further affecting work ability, obesity is also prone to many symptoms, including edema, cardiac hypertrophy, fatty liver, bile and urinary calculi, and musculoskeletal pain. Disease, gynecological breast Or uterine tumor, hyperuricemia (gout), hyperlipidemia, angina, diabetes, high blood pressure, stroke, etc. According to the latest statistics of the Department of Health, the proportion of adult males who are overweight or obese in China has reached 51%, and women have reached 36%. The ratio of overweight or obesity in primary school children in Yuguo is 29% for boys and 21.0% for girls. The ratio of overweight and obesity continues to rise. In addition, among the top ten causes of death among Chinese people, malignant tumors, heart disease, cerebrovascular disease, diabetes, chronic lower respiratory disease, hypertension, chronic liver disease, liver cirrhosis, and chronic kidney disease The cause of death is related to obesity, so maintaining a healthy weight is the goal of joint efforts.
目前減肥方法琳瑯滿目、千奇百怪,且都屬於非健康的減肥方法,且藉由規律的運動以及改變飲食才是最主要的方法。然而,減肥者往往缺乏耐心,無法持續運動以及對美食沒有抵抗力是他們所面臨的最大問題,因此減肥產品便成為減肥者的最佳選擇。常見的減肥產品,例如衛生署核准的減肥藥phenyl propanolamine,係能促進體內的新陳代謝率、增加熱量的消耗、減少食慾等,並且於短時間內即可看到減重的效果,不過同時伴隨心悸、嘔吐、失眠等副作用的現象,嚴重者還會產生厭食症,因此讓使用者雖然成功減重,但也賠上了健康。 At present, the weight-loss methods are dazzling and ridiculous, and they all belong to unhealthy methods of weight loss, and regular exercise and change of diet are the most important methods. However, dieters often lack patience, lack of exercise and resistance to food are the biggest problems they face, so weight loss products are the best choice for dieters. Common weight loss products, such as the phenyl propanolamine approved by the Department of Health, can promote the body's metabolic rate, increase calorie consumption, reduce appetite, etc., and can see the effect of weight loss in a short period of time, but at the same time with heart palpitations Side effects such as vomiting, insomnia, and severe anorexia can cause the user to lose weight, but they also lose health.
因此,提供一種健康的減肥方法實為刻不容緩的一件事情。一般人減肥都只會著重在減「重」而非減「肥」,也就是說體脂肪比率不一定會有改變,然而降低體內三酸甘油脂含量,才表示真正的減到肥肉,且同時提高體內新陳代謝能力,將更容易達到減肥目標。 Therefore, providing a healthy way to lose weight is an urgent task. The average person loses weight and only focuses on reducing "heavy" rather than "fat". That is to say, the body fat ratio does not necessarily change. However, lowering the body's triglyceride content means that the fat is actually reduced to fat. Improve your body's metabolism and make it easier to achieve your weight loss goals.
為解決上述的問題,本發明提供一種植物萃取物和枯草桿菌的組合物,可以有效地讓使用者達到健康減重的目的。 In order to solve the above problems, the present invention provides a composition of a plant extract and Bacillus subtilis, which can effectively achieve the purpose of healthy weight loss for a user.
本發明提供一種抑制脂肪蓄積的組合物,其係包含毛喉鞘蕊花萃取物以及枯草桿菌BS-139,該枯草桿菌係保存於財團法人食品工業發展研究所寄存編號BCRC 910457之菌株。 The present invention provides a composition for inhibiting fat accumulation, which comprises an extract of Coleus formosana and Bacillus subtilis BS-139, which is a strain deposited in the No. BCRC 910457 of the Institute of Food Industry Development.
本發明一實施例中,其中該毛喉鞘蕊花萃取物含量約為0.01wt%~0.05wt%,且該枯草桿菌BS139含量約為0.02wt%~0.08wt%。 In an embodiment of the invention, the content of the extract of the genus Corydalis is about 0.01 wt% to 0.05 wt%, and the content of the B. subtilis BS 139 is about 0.02 wt% to 0.08 wt%.
本發明一實施例中,其中該組合物可有效減少動物或人類的體重。 In one embodiment of the invention, the composition is effective to reduce the body weight of an animal or a human.
本發明一實施例中,其中該組合物可有效降低動物或人類血液中的三酸甘油脂。 In one embodiment of the invention, the composition is effective to reduce triglycerides in the blood of animals or humans.
本發明一實施例中,其中該組合物可以降低動物或人類食物熱量的攝取。 In one embodiment of the invention, the composition reduces the uptake of calories in animal or human food.
本發明一實施例中,其中該組合物可應用於藥品、健康食品、保健食品、食品和飼料。 In an embodiment of the invention, the composition is applicable to pharmaceuticals, health foods, health foods, foods and feeds.
本發明另提供一種植物萃取物的組合物用於製備治療肥胖之用途,其中該萃取物的組合包含:有效劑量的毛喉鞘蕊花萃取物,含量為0.01wt%~0.05wt%;以及有效劑量的枯草桿菌BS-139,該枯草桿菌係保存於中華民國食品工業發展研究所生物資源保存及研究中心寄存編號BCRC 910457之菌株,含量為0.02wt%~0.08wt%。 The invention further provides a use of a composition of a plant extract for the preparation of a medicament for treating obesity, wherein the combination of the extract comprises: an effective dose of the extract of Coleus forsythiae, the content is 0.01 wt% to 0.05 wt%; The dose of Bacillus subtilis BS-139, which is stored in the Bioresource Conservation and Research Center of the Republic of China Food Industry Development Institute, is the strain of BCRC 910457, and the content is 0.02wt%~0.08wt%.
第1圖顯示餵飼不同飼料的雌性大鼠其體重隨天數的變化。 Figure 1 shows the change in body weight over days in female rats fed different diets.
第2圖顯示餵飼不同飼料的雌性大鼠其各週增重的變化。 Figure 2 shows the changes in weekly weight gain of female rats fed different diets.
第3圖顯示餵飼不同飼料的雌性大鼠其各週採食量的變化。 Figure 3 shows the changes in feed intake per week for female rats fed different diets.
第4圖顯示餵飼不同飼料的雌性大鼠其飼料效率的結果。 Figure 4 shows the results of feed efficiency of female rats fed different feeds.
實驗動物與飼養環境 Laboratory animals and breeding environment
實驗動物採用五週齡大的Wistar雌性大鼠,共18隻,每隻約160公克,並飼養在財團法人農業科技研究院動物科技研究所齧齒類動物房。嚙齒類動物房溫度控制在約23℃(20-26℃)及提供12小時光照之光照週期條件,實驗大鼠飼養於經高溫高壓滅菌後的飼養籠,且飲水方式係以高壓滅菌水瓶供應。 The experimental animals used a five-week-old Wistar female rat, a total of 18 rats, each weighing about 160 grams, and were housed in a rodent house of the Institute of Animal Science and Technology of the Institute of Agricultural Science and Technology. The rodent chamber temperature was controlled at about 23 ° C (20-26 ° C) and provided with a 12-hour light photoperiod condition. The experimental rats were housed in a cage that was autoclaved and the drinking water was supplied in an autoclaved water bottle.
建立不同飼料組的Wistar雌性大鼠 Establishment of Wistar female rats of different feed groups
實驗大鼠以2隻一籠方式進行飼養,並標示動物號、籠號,經7天適應期後進行六週試驗。大鼠隨機分配至對照組、加油組或加油與藥組,每組各6隻,共3籠。對照組餵飼進口大鼠專用粒狀飼料(Lab Diet® 5001 Rodent Diet)。加油組餵飼外加10%沙拉油的高油脂飼料,其中高油脂飼料製作係以進口大鼠專用粒狀飼料粉碎後添加10%沙拉油,再塑形成塊狀,且為避免油脂氧化問題,高油脂飼料在餵飼前二天才開始準備。加油與藥組也餵飼高油脂飼料,再添加枯草桿菌BS-139(Bacillus subtilis)與毛喉鞘蕊花萃取物(Forskohlii extract)。飼料主要營養成分列於表一,其中之數據為一平均值。 The experimental rats were reared in two cages, and the animal number and the cage number were marked, and the test was carried out for six weeks after the 7-day acclimation period. Rats were randomly assigned to the control group, the refueling group, or the refueling and drug group, with 6 in each group, for a total of 3 cages. The control group was fed a special granular feed (Lab Diet® 5001 Rodent Diet) from imported rats. The refueling group is fed with high-fat feed supplemented with 10% salad oil. The high-fat feed production is pulverized with imported granular feed for rats, and then 10% salad oil is added to form a block, and to avoid the problem of oil oxidation, high The fat feed began to be prepared two days before feeding. The refueling and drug groups were also fed a high-fat diet, and Bacillus subtilis and Forskohlii extract were added. The main nutrients of the feed are listed in Table 1, where the data is an average.
試驗分析 Test analysis
大鼠分別在試驗開始(即為第1天)及每週秤重,並記錄每隻大鼠各週飼料消耗量。試驗期間每日進行臨床觀察,記錄動物是否有臨床症狀或死亡。試驗結束時(即為第42天),自尾靜脈採血,以血液生化分析儀(Kodak Ektachem DT-II System,Rochester,NY,USA)測葡萄糖、三酸甘油脂、天門冬胺酸轉胺酵素(AST)及丙胺酸轉胺酵素(ALT)的含量。隨後,犧牲大鼠進行肉眼病理觀察,並記錄組織重量。 Rats were weighed at the beginning of the trial (ie day 1) and weekly, and the weekly feed consumption of each rat was recorded. Clinical observations were performed daily during the trial to document whether the animals had clinical signs or death. At the end of the experiment (ie day 42), blood was collected from the tail vein and glucose, triglyceride, aspartate transaminase was measured by a blood biochemical analyzer (Kodak Ektachem DT-II System, Rochester, NY, USA). (AST) and alanine transaminase (ALT) content. Subsequently, the rats were sacrificed for gross pathological observation and the tissue weight was recorded.
實例一:飼料與能量攝取量 Example 1: Feed and energy intake
請參考表二,試驗期間,各組飼料攝取量略有變化,但差異未達統計顯著性(P>0.05),其中表二之數值為一平均值。 Please refer to Table 2, during the test period, the feed intake of each group changed slightly, but the difference did not reach statistical significance (P>0.05), and the value of Table 2 was an average value.
與餵飼專用飼料之對照組比較,加油組係餵飼外加10%沙拉油的高油脂飼料,其第一週的飼料攝取量較對照組低8.6%,第二週更減少27.6%,之後飼料攝取量較對照組高,第三至第六週間增加7-19%。加油組第一與第二週飼料攝取量似乎受到油脂添加的影響而降低,然而經過二週適應,隨後四週的飼料攝取量都比對照組高。在整個試驗期間,加油組的總飼料攝取量與對照組相近,但總能量攝取量較對照組高15.6%,符合應用高油脂飼料增加大鼠能量攝取的目的。 Compared with the control group for feeding special feed, the refueling group was fed with high-fat feed with 10% salad oil. The feed intake in the first week was 8.6% lower than that of the control group, and the second week was decreased by 27.6%. The intake was higher than the control group and increased by 7-19% between the third and sixth weeks. Feed intake in the first and second weeks of the refueling group appeared to be reduced by the effect of oil addition. However, after two weeks of adaptation, the feed intake for the next four weeks was higher than that of the control group. During the whole experiment period, the total feed intake of the refueling group was similar to that of the control group, but the total energy intake was 15.6% higher than that of the control group, which was consistent with the purpose of increasing the energy intake of rats by applying high fat diet.
加油與藥組係餵飼高油脂飼料,再添加枯草桿菌BS-139與毛喉鞘蕊花萃取物,攝取量變化與加油組並不一致。加油與藥組第一週飼料攝取量較對照組高17.2%、加油組高28.3%,第二週飼料攝取量與加油組相似,但比對照組低27.6%,之後,第五週比對照組高28.4%外,第四、六週的飼料攝取量比對照組低7%。添加枯草桿菌與毛喉鞘蕊花萃取的組合物似乎在於隔一週後有抑制大鼠攝取飼料的情形(即為第二、四、六週)。在整個試驗期間,加油與藥組總飼料攝取量與對照組相近,而總能量攝取量較對照組高13%,但比加油組低2.6%。 The refueling and drug groups were fed with high-fat diet, and then B. subtilis BS-139 and Coleus forsythiae extract were added. The change in intake was not consistent with the refueling group. The feed intake in the first week of refueling and drug group was 17.2% higher than that of the control group and 28.3% higher in the refueling group. The feed intake in the second week was similar to that in the refueling group, but was 27.6% lower than that in the control group. At 28.4% higher, the feed intake in the fourth and sixth weeks was 7% lower than that in the control group. The combination of Bacillus subtilis and Coleus formosanus extraction appears to have a situation in which the rats are prevented from ingesting the feed after one week (i.e., the second, fourth, and sixth weeks). During the whole experiment period, the total feed intake of the refueling and drug groups was similar to that of the control group, while the total energy intake was 13% higher than the control group, but 2.6% lower than the refueling group.
實例二:生長曲線與體重變化 Example 2: Growth curve and weight change
請參考第1圖,試驗開始時,各組大鼠平均體重相近似,約177公克,隨著天數的增加,各組大鼠的體重皆增加。其中,對照組增加的體重隨天數增加而逐較趨緩,加油組的生長曲線與對照組不同。值得注意的是,加油與藥組的體重在第21天開始即比對照組與加油組低,與對照組和加油組的差距也隨著時間而增加,且並非以等距離方式增加此差距。 Please refer to Figure 1. At the beginning of the experiment, the average body weight of each group was similar, about 177 grams. As the number of days increased, the body weight of each group increased. Among them, the increased body weight of the control group gradually slowed down with the increase of the number of days, and the growth curve of the fueling group was different from that of the control group. It is worth noting that the weight of the refueling and drug groups was lower than that of the control group and the refueling group on the 21st day, and the gap between the control group and the refueling group also increased with time, and the gap was not increased in an equidistant manner.
請參考表三,其中表三之數據為一平均值,同一列不同上標表示顯著差異,a與b之P值小於0.05,a與c之P值小於0.01以及a與d之P值小於0.001。 Please refer to Table 3, where the data in Table 3 is an average value, and the different superscripts in the same column indicate significant differences. The P values of a and b are less than 0.05, the P values of a and c are less than 0.01, and the P values of a and d are less than 0.001. .
對照組在第一、二週時增重超過22g,第三、四週時為16.5g,第五週降為7.7g,第六週僅3.2g。加油組於第一週增重僅有8g,顯著低於對照組(P<0.05),相反的,試驗第二週增重顯著較對照組高,達42.3g(P<0.001),隨後的3-6週,二組間各週增重並無差異。加油與藥組第一週增重與其他二組無差異,但第二週顯著高於對照組,達35.2g(P<0.01),第三週時,加油與藥組僅增重5.2g,顯著低於對照組與加油組(P<0.01),隨後的4-6週增重與其他二組無差異,但都比其他二組低。於試驗期間,對照組與加油組總增重分別為90.2g和98.5g,相對的,加油與藥組總增重僅74.8g,顯著低於其他二組(P<0.05),結果顯示加油與藥組從第3週開始其體重曲線 低於對照組和加油組,證明餵食含枯草桿菌與毛喉鞘蕊花萃取的組合物之飼料有助於減輕體重。 The control group gained more than 22 g in the first and second weeks, 16.5 g in the third and fourth weeks, 7.7 g in the fifth week, and only 3.2 g in the sixth week. In the first week, the weight gain of the refueling group was only 8g, which was significantly lower than that of the control group (P<0.05). On the contrary, the weight gain in the second week of the test was significantly higher than that of the control group, reaching 42.3g (P<0.001), followed by 3 -6 weeks, there was no difference in weight gain between the two groups. The first week of weight gain in the refueling and drug groups did not differ from the other two groups, but the second week was significantly higher than the control group, reaching 35.2 g (P<0.01). At the third week, the refueling and drug groups only gained 5.2 g. Significantly lower than the control group and the refueling group (P<0.01), the subsequent 4-6 weeks weight gain was no different from the other two groups, but both were lower than the other two groups. During the test period, the total weight gain of the control group and the refueling group were 90.2 g and 98.5 g, respectively. In contrast, the total weight gain of the refueling and drug groups was only 74.8 g, which was significantly lower than the other two groups (P<0.05). The drug group began its weight curve from week 3 Below the control and refueling groups, it was demonstrated that feeding the feed containing the composition of B. subtilis and Coleus formosan extraction helped to lose weight.
請參考第2、3圖,大鼠餵飼高油脂飼料初期可能因適應問題而減少攝取量,進一步降低增重(第一週)。但加油與藥組第一週的飼料攝取量高於對照組17%,但增重卻低40%,表示添加枯草桿菌與毛喉鞘蕊花萃取的組合物,似乎具有控制體重增加的作用。除第二週外,相似的情形在第三、四、五、六週也觀察到。與對照組比較,第三週加油與藥組攝取量高3.8%,但增重低68.5%,第五週試驗組攝取量高28.4%,但增重低38.7%。與加油組比較,第三週試驗組攝取量低8.6%,但增重低74.3%,第五週試驗組攝取量高7.4%,但增重低39.7%。結果顯示加油與藥組採食量上升,但體重較對照組和加油組不增反減,證明大鼠餵食含枯草桿菌與毛喉鞘蕊花萃取的組合物之飼料,能有效控制體重的增加。 Please refer to Figures 2 and 3. Rats fed high-fat diets may reduce their intake due to adaptation problems and further reduce weight gain (first week). However, the feed intake in the first week of refueling and drug group was higher than that of the control group by 17%, but the weight gain was 40% lower, indicating that the addition of Bacillus subtilis and Coleus forsythiae extract composition seems to have the effect of controlling weight gain. A similar situation was observed in the third, fourth, fifth, and sixth weeks, except for the second week. Compared with the control group, the third week of refueling and drug group intake was 3.8% higher, but the weight gain was 68.5% lower. In the fifth week, the test group intake was 28.4% higher, but the weight gain was 38.7% lower. Compared with the refueling group, the intake of the test group in the third week was 8.6% lower, but the weight gain was 74.3% lower. In the fifth week, the intake of the test group was 7.4% higher, but the weight gain was 39.7% lower. The results showed that the feed intake of the refueling and the drug group increased, but the body weight did not increase or decrease compared with the control group and the refueling group. It was proved that the feed of the composition containing the extract of Bacillus subtilis and Coleus formosanus can effectively control the increase of body weight.
實例三:組織重量 Example 3: Tissue weight
試驗期間,所有動物皆健康,無死亡情形,也沒有觀察到任何臨床症狀。請參考表四,其中表四之數據為一平均值,同一行不同上標表示顯著差異,a與b之P值小於0.05以及a與c之P值小於0.01。動物犧牲後,各組間之心臟、腎臟重量沒有差異。然而,加油組腹部脂肪重量較對照組高58.5%(P<0.05),加油與藥組同樣餵飼高油脂飼料,但腹部脂肪重比加油組降低44.5%(P<0.01),也比對照組低12.1%(P>0.05)。加油與藥組中係添加枯草桿菌與毛喉鞘蕊花萃取的組合物,在相似的能量攝取條件下,抑制了大鼠腹部脂肪的蓄積,且明顯比對照組和加油組少,有效證明本發明的組合物有效減少脂肪吸收、降低脂肪累積。 During the test, all animals were healthy, no deaths, and no clinical symptoms were observed. Please refer to Table 4, where the data in Table 4 is an average value, and the different superscripts on the same line indicate significant differences. The P values of a and b are less than 0.05 and the P values of a and c are less than 0.01. After the animals were sacrificed, there was no difference in heart and kidney weight between the groups. However, the abdominal fat weight of the refueling group was 58.5% higher than that of the control group (P<0.05). The refueling and the drug group were also fed with high-fat diet, but the abdominal fat weight was 44.5% lower than that of the refueling group (P<0.01). 12.1% lower (P>0.05). In the refueling and drug group, the combination of Bacillus subtilis and Coleus formosanus extract inhibited the accumulation of abdominal fat in rats under similar energy intake conditions, and was significantly less than the control group and the refueling group. The composition of the invention is effective in reducing fat absorption and reducing fat accumulation.
實例四:血液化學變化 Example 4: Blood chemistry changes
請參考表五,其中表五之數據為一平均值,同一行不同上標表示顯著差異,a與c之P值小於0.01。與對照組比較,加油組高油脂飼料降低血液中葡萄糖濃度(P<0.01),但略為提高三酸甘油酯濃度(16.5%)。加油與藥組的葡萄糖濃度也較對照組低(P<0.01),但其三酸甘油脂增加的幅度(5.5%)較加油組減少11%。由此可知,當吃的食物油脂較多時,餵食添加枯草桿菌與毛喉鞘蕊花萃取的組合物能降低三酸甘油脂,其作用機制可能與葡萄糖生成無關,但與脂肪代謝、合成有關。 Please refer to Table 5, where the data in Table 5 is an average value, and the different superscripts on the same line indicate significant differences, and the P values of a and c are less than 0.01. Compared with the control group, the high-fat diet in the refueling group reduced the blood glucose concentration (P<0.01), but slightly increased the triglyceride concentration (16.5%). The glucose concentration in the refueling and drug groups was also lower than that in the control group (P<0.01), but the increase in triglyceride (5.5%) was 11% lower than that in the refueling group. It can be seen that when the foods and fats eaten are more, the combination of Bacillus subtilis and Coleus formosan extract can reduce triglyceride, and its mechanism may be independent of glucose production, but related to fat metabolism and synthesis. .
此外,天門冬胺酸轉胺酵素含量及丙胺酸轉胺酵素含量係為臨床上常用來評估肝細胞受損程度及肝病的急慢性分類,也是肝病治療成效的重要指標。丙胺酸轉胺酵素大量存在於肝臟中,而天門冬胺 酸轉胺酵素則存於心臟及肌肉中。當這些部位的細胞受損時,特別是肝細胞受損,血清中之天門冬胺酸轉胺酵素及丙胺酸轉胺酵素的活性會明顯升高。本實施例中,可看見加油組之天門冬胺酸轉胺酵素雖然含量較對照組低,但不影響高密度膽固醇與丙胺酸轉胺酵素;但加油組天門冬胺酸轉胺酵素含量略為提高,而試驗組添加物有降低天門冬胺酸轉胺酵素的情形,但各組間差異不顯著。 In addition, aspartic acid transaminase content and alanine transaminase content are clinically used to assess the degree of hepatocyte damage and acute and chronic classification of liver disease, and are also an important indicator of the effectiveness of liver disease treatment. Alanine transaminase is abundantly present in the liver, while aspartame Acid transaminase is found in the heart and muscles. When cells in these areas are damaged, especially liver cells, the activity of aspartate transaminase and alanine transaminase in serum is significantly increased. In this example, it can be seen that although the content of aspartic acid transaminase in the refueling group is lower than that of the control group, it does not affect high-density cholesterol and alanine transaminase; however, the asparagine transaminase content in the refueling group is slightly increased. However, the test group additions reduced the aspartate transaminase, but the difference between the groups was not significant.
實例五:飼料效率 Example 5: Feed efficiency
請參考第4圖,以總增重除以總飼料攝取量,計算飼料轉換成體重的效率,對照組與加油組數據相近似。加油與藥組的飼料效率較其他二組低近19%,表示餵飼枯草桿菌BS-139與毛喉鞘蕊花萃取物的大鼠需多攝取19%的飼料,才能達到相似的體重增加。因此,本發明含枯草桿菌BS-139與毛喉鞘蕊花的萃取組合物有降低飼料營養吸收、轉換成體組成的作用。 Please refer to Figure 4 to calculate the efficiency of the conversion of feed into body weight by dividing the total weight gain by the total feed intake. The control group is similar to the fueling group data. The feed efficiency of the refueling and drug groups was nearly 19% lower than that of the other two groups, indicating that rats fed B. subtilis BS-139 and Coleus forsythiae extracts required 19% more feed to achieve similar weight gain. Therefore, the extracting composition of the present invention containing Bacillus subtilis BS-139 and Coleus formosana has the effect of reducing nutrient absorption of the feed and converting into a body composition.
根據本發明之實驗,攝取高油脂飼料的Wistar雌性大鼠會增加體重及腹部脂肪蓄積,同時攝取枯草桿菌BS-139與毛喉鞘蕊花的萃取組合物能控制高油脂飼料誘發的增重(24.1%),並抑制脂肪代謝(三酸甘油酯)和腹部脂肪量(44.5%),降低的幅度比正常飼料組增加的體重及腹部脂肪量要大,且於六週後在餵飼高油脂飼料和添加萃取組合物的大鼠,沒有任何肉眼可觀察之臨床症狀(指嚴重副作用)。顯然本發明之組合物能抑制體重增加、減少脂肪吸收及降低脂肪蓄積,且不會改變使用者的食慾,亦沒有副作用的產生,係以健康型態來控制體重的效果。 According to the experiment of the present invention, Wistar female rats ingesting high fat diets can increase body weight and abdominal fat accumulation, and the extract composition of Bacillus subtilis BS-139 and Coleus forsythia can control the weight gain induced by high fat diet ( 24.1%), and inhibited fat metabolism (triglyceride) and abdominal fat mass (44.5%), the magnitude of the decrease was greater than the increase in body weight and abdominal fat in the normal diet group, and the high fat was fed after six weeks. The feed and the rats to which the extract composition was added did not have any clinical symptoms (referred to as severe side effects) which were observable by the naked eye. It is apparent that the composition of the present invention inhibits weight gain, reduces fat absorption, and reduces fat accumulation without changing the appetite of the user, and has no side effects, and controls the body weight in a healthy manner.
國內寄存資訊【請依寄存機構、日期、號碼順序註記】 Domestic registration information [please note according to the registration authority, date, number order]
財團法人食品工業研究所 Food Industry Research Institute
民國103年6月27日 June 27, 103, Republic of China
寄存編號BCRC 910457 Deposit number BCRC 910457
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW103142403A TWI535448B (en) | 2014-12-05 | 2014-12-05 | A composition of decreasing the accumulation of fat and use thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW103142403A TWI535448B (en) | 2014-12-05 | 2014-12-05 | A composition of decreasing the accumulation of fat and use thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
TWI535448B true TWI535448B (en) | 2016-06-01 |
TW201620539A TW201620539A (en) | 2016-06-16 |
Family
ID=56755187
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW103142403A TWI535448B (en) | 2014-12-05 | 2014-12-05 | A composition of decreasing the accumulation of fat and use thereof |
Country Status (1)
Country | Link |
---|---|
TW (1) | TWI535448B (en) |
-
2014
- 2014-12-05 TW TW103142403A patent/TWI535448B/en active
Also Published As
Publication number | Publication date |
---|---|
TW201620539A (en) | 2016-06-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104012807B (en) | A kind of livestock and poultry antiviral B B-complex premixed feed preparation and preparation method and application thereof | |
Malyar et al. | Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress | |
Lee et al. | Effect of Chlorella vulgaris on lipid metabolism in Wistar rats fed high fat diet | |
CN102524536B (en) | Application of theaflavin as feed additive and corresponding feed | |
CN101310621B (en) | Weight-reducing food and production method thereof | |
WO2014190935A1 (en) | Multi-functional composition and preparation method and application thereof | |
CN100353979C (en) | Chinese herbal medicinal immune intensifier mode from left-eyed flounder and right-eyed flounder | |
Yang et al. | Effects of chromium-enriched Bacillus subtilis KT260179 supplementation on growth performance, caecal microbiology, tissue chromium level, insulin receptor expression and plasma biochemical profile of mice under heat stress | |
CN102552411A (en) | Chinese herbal medicine immunopotentiator for sturgeon fingerling | |
JP2020147582A (en) | Composition for arthritis, mobility and delay ageing | |
TWI627959B (en) | Use of lactobacillus reuteri gmnl-263 for manufacturing composition for increasing expression of ldl-r and cyp7a1 in liver in high-fat diet individual | |
CN103300250A (en) | Feed capable of reducing cholesterol content of egg and meat products | |
WO2021203661A1 (en) | Seaweed fruit and vegetable formula for assisting in reducing fat and losing weight | |
TWI535448B (en) | A composition of decreasing the accumulation of fat and use thereof | |
KR20120044807A (en) | Composition comprising chlorella for improving liver function or relieving hangover | |
Shen et al. | Effect of echinacea purpurea extract given in drinking water on performance, slaughter variables, and meat quality of broilers | |
KR20150015305A (en) | Pharmaceutical composition comprising the lees extract of korean rice wine as an effective component for prevention or treatment of obesity and health functional food comprising the same | |
CN103491959A (en) | Trpm5 inhibitors support body weight reduction without reducing food intake | |
US20170296608A1 (en) | Composition of decreasing the accumulation of fat and use thereof | |
KR101125185B1 (en) | a-amlyase inhibitor containing the extracts from oceanic life species for anti-obesity | |
JP2011037811A (en) | Fat-decreasing composition | |
Susanto et al. | Efficacy of moringa oleifera leaf powder as nutrigenomic therapy against malnutrition and metabolic perturbation related diseases: a preliminary study of madura islands variety | |
CN107173605A (en) | A kind of feed for being used to slow down summer brood time meat duck heat stress | |
TW201904597A (en) | Purslane processing products, methods for producing processed purslane, supplements, pharmaceuticals, intestinal mucosal protective agents, and intestinal tract preparations | |
CN103976356B (en) | A kind of diet food and preparation method thereof |