TWI513475B - Rhodiola composition with functions of brightening, moisturizing, unwrinkling, compacting, and softening skin - Google Patents

Description

Bright white, moisturizing, smoothing fine lines, firming skin and conditioning skin Rhodiola composition

The present invention relates to a composition, and more particularly to a Rhodiola composition having bright white, moisturizing, smoothing, fine lines, firming and conditioning functions.

Beauty is the nature of human beings. Among them, the skin is white and more popular. Not only women are crazy about pursuing whitening. In recent years, the index of boys’ love for girls has risen steadily. Huamei is no longer just a kanban on the Internet or TV. Characters, but new goals that men practice. In fact, the reason why the skin will turn black is that the skin is directly or indirectly exposed to sunlight every day. In order to protect the skin from the ultraviolet rays in the sun, melanocytes in the skin will synthesize melanin. To defend against the ultraviolet rays in the sun.

Melanocytes, also known as melanin protoplasts or melanine mother cells, are located in the lowest basal layer of the epidermal layer of the skin. The melanocytes in human skin are specialized from nerve cells. Therefore, melanocytes are like starfish in the shape of neurocollagen cells. They often grow branches and are surrounded by other surrounding cells to absorb nutrients. Melanin formed in melanocytes will be coated In a cell called a melanosome, it is sent to the epidermis to form a skin tone. The process of melanin formation is a sustained and balanced response in normal physiology. The melanosome secreted by melanocytes gradually moves up through the renewal and metabolism of the epidermis, and finally the horny layer with the aging is detached.

When the epidermis removes melanin at a rate equal to the rate at which melanin produces melanin and secretes melanin in the basal layer, the appearance of the skin tone maintains a degree of uniformity (ie, the appearance does not continue to darken or gradually whiten). However, under certain special circumstances (such as a large amount of ultraviolet light), the rate of pigmentation is accelerated, thereby destroying the aforementioned equilibrium state, resulting in melanin accumulation. The skin not only continues to darken, but also makes the overall skin tone uneven. In severe cases, spots such as dark spots, freckles, liver spots and age spots are formed on the skin. Although the main purpose of melanin on human skin is to protect against ultraviolet rays in sunlight, excessive hyperplasia of melanin causes aesthetic problems. The desire to have a healthy and fair skin is what many people yearn for, so the development of whitening cosmetics has become an important issue.

Recently, there have been more and more researches on the development and mechanism of action of Chinese herbal medicines. In addition, the use of Chinese herbal medicines in cosmetics has been used to enhance the activity of active ingredients, and its side effects are also less. Therefore, more and more cosmetics and skin care products are used to add Chinese herbal medicines. Extract ingredients to increase skin whitening and anti-oxidant properties. In addition to ancient records, the clinical use of Chinese herbal medicines can also be used to find new active ingredients by extraction, separation and purification combined with screening of activity evaluation.

However, some Chinese herbal medicines have been found to have whitening and anti-oxidation effects, but all have a single effect and limited efficacy, and can not achieve good whitening and anti-oxidation effects. Therefore, finding the best Chinese herbal medicine composition, and the proportion of single Chinese herbal medicine, is an important topic of Chinese herbal health care products.

In view of the above problems of the prior art, the object of the present invention is to provide a Rhodiola composition having bright white, moisturizing, smoothing fine lines, firming skin and conditioning and softening function, and the Rhodiola composition comprises Specific Chinese herbal medicines and various Chinese herbal medicines are combined in a specific ratio to achieve optimal whitening (brightening), moisturizing, anti-oxidation (smoothing fine lines, firming skin), anti-photoaging and antibacterial (conditioning softening) The effect of the skin).

In accordance with the purpose of the present invention, a Rhodiola composition comprising Rhodiola and a Chinese herbal compound is provided. The Chinese herbal compound contains at least red peony, mulberry leaves, alfalfa and licorice. Among them, the weight percentage of Rhodiola is 60~95wt%, and the weight percentage of Chinese herbal compound is 5~40wt%.

Further, the present invention further provides a method for producing a Rhodiola composition comprising the following steps. First, the Rhodiola and Chinese herbal medicines are extracted with organic matter or water to obtain a mixed extract, and the Chinese herbal compound contains at least red peony, mulberry leaves, alfalfa and licorice. Next, the mixed extract is concentrated, and the concentrated mixed extract is dissolved in a solvent to form a Rhodiola composition. Among them, the weight percentage of Rhodiola is 60~95wt%, and the weight percentage of Chinese herbal compound is 5~40wt%.

In view of the above, the Rhodiola composition according to the present invention may have one or more of the following advantages:

(1) The Rhodiola composition of the present invention comprises Rhodiola, Radix Paeoniae, Mulberry Leaf, Lycium and Licorice Herbs, and is composed in a specific ratio, so that the Rhodiola composition of the present invention not only retains the efficacy of the single Chinese herbal medicine It also has the effect of adding various Chinese herbal medicines.

(2) After using the Rhodiola composition of the present invention, the user can suppress melanin synthesis of the skin of the user and also resist the effect of oxidative stress.

S11~S13‧‧‧Steps

Fig. 1 is a flow chart showing the manufacture of the Rhodiola composition of the present invention.

Fig. 2 is a graph showing the results of moisturizing test results of the Rhodiola composition of the present invention.

The invention will be further described in detail by the following preferred embodiments and the accompanying drawings. It should be noted that the experimental data disclosed in the following embodiments are for explaining the technical features of the present invention, and are not intended to limit the manner in which they can be implemented.

Example 1: Composition of the Rhodiola composition of the present invention

The Rhodiola composition of the present invention comprises Rhodiola and a Chinese herbal compound. The Chinese herbal compound contains at least red peony, mulberry leaves, alfalfa and licorice. Among them, the percentage of weight of Rhodiola is 60~95wt%, while that of Chinese herbal medicine is 5~40wt%. Preferably, the weight percentage of Rhodiola may be 75 to 90% by weight, and the weight percentage of the Chinese herbal compound may be 10 to 25 wt%. The ratios of red peony, mulberry leaves, alfalfa and licorice are the same, that is, for example, when the weight percentage of the Chinese herbal compound is 18% by weight, the red peony, mulberry leaves, alfalfa and licorice each account for 4.5% by weight.

Among them, the main component of the Rhodiola composition of the present invention is Rhodiola, which is a kind of herbal medicine, also known as Tibetan god grass. In the "Compendium of Materia Medica" and "Shen Nong's Herbal Classic", there are records of Rhodiola: Days, non-toxic, multi-service, long-term service does not hurt people, for the sake of light body and good fortune, not old age. Rhodiola is the root and rhizome of Sedum, which is sweet, bitter and astringent. It is red because it contains anthocyanidin. The flavonoids in the ingredients can immediately activate the mitochondria and recreate energy. The various phenolic compounds contained in the plant are strong antioxidants, in addition to significantly improving the body's tolerance to hypoxia, and can promote the body's adaptation to anoxic environment and delay the occurrence and development of fatigue.

In addition, licorice alias sweet grass root, dense grass, known as the king of Chinese herbal medicine, is the most widely used medicine in Chinese herbal medicine, has been used as a raw material for whitening cosmetics since ancient times, many well-known whitening makeup The product contains licorice. Licorice has anti-inflammatory, analgesic, detoxifying and antibacterial effects. In addition, the protein in the licorice component and its hydrolyzed amino acid are used in cosmetics, have a moisturizing effect on skin and hair, and have a repairing effect on damaged skin and hair. In general, licorice is a good cosmetic additive, which has a good effect on inhibiting the formation of melanin, inhibiting the activity of tyrosinase and anti-oxidation, and has both whitening and anti-aging effects.

Akasaka is also known as wood red peony, red peony, and red peony. Function to clear heat and cool blood, loose silt and relieve pain. For warming hair spots, red eyes and swelling, menstrual dysmenorrhea, symptoms, abdominal pain, puff injury, swollen sores and other symptoms. Modern research results show that red peony mainly contains peony, peony and other ingredients, and has many pharmacological effects such as sedative, antispasmodic, anti-inflammatory, anti-emergency ulcer, and expansion of coronary vessels.

茯苓 is also known as Yunxiao, Bailu. Modern medical research, polysaccharides can enhance the body's immune function, improve the body's disease resistance, and can significantly increase the phagocytic function of peritoneal macrophages, enhance T cell function, and have a strong anti-tumor effect. In addition, sputum also has the effect of enhancing myocardial contractility, inhibiting the occurrence of gastric ulcer, protecting the liver and inhibiting bacteria.

Mulberry leaves are Moraceae. In Chinese medicine treatment, mulberry leaves are used as medicines for improving diabetes and various other intractable diseases. Mulberry leaves have antitussive, detoxification, treatment of dizziness, elimination of eye fatigue, swelling, clearing blood, curing dysentery, liver and skin. In recent years, studies have confirmed that mulberry leaves are indeed natural and skin care products with good quality and low price, especially for facial acne and brown spots. Mulberry leaves are also rich in copper, which has the effect of preventing hair and skin whitening. Long-term adherence can make white hair turn black, face is rosy, and can prevent the generation of less white hair.

In addition, the above-mentioned Chinese herbal medicine combination may further comprise astragalus, Chuanxiong, Salvia miltiorrhiza or a combination thereof. The Rhodiola composition of the present invention can be formed into a powder form, a granule form, an emulsion form or a liquid form. It may also be added with pharmaceutically acceptable excipients or additives, such as optional free toners, fragrances, preservatives, antioxidants, chelating agents, penetrants, lubricants, lozenge adjuvants, colorants, moisturizers. a group of agents, binders, and pharmaceutically compatible carriers, in the form of capsules, tablets, troches, pastes, and the like, Further for the user to use. Further, the Rhodiola composition of the present invention can be further prepared into an emulsion or a gel, such as a lotion, a lotion, a mask, a gel, or the like, for application or application to the skin.

Example 2: Method for producing Rhodiola composition of the present invention

Please refer to Fig. 1, which is a flow chart for the manufacture of the Rhodiola composition of the present invention. In the figure, in step S11, a Rhodiola and a Chinese herbal compound are extracted by using an organic matter or water to obtain a mixed extract, and the Chinese herbal compound comprises at least red peony, mulberry leaves, alfalfa and licorice. In step S12, the mixed extract is concentrated. In step S13, the concentrated mixed extract is dissolved in a solvent to obtain the Rhodiola composition of the present invention. The weight percentage of Rhodiola is 60-95 wt%, and the compound of Chinese herbal medicine is 5-40 wt%, and the red peony, mulberry leaf, alfalfa and licorice are evenly distributed in the Chinese herbal compound, and the description thereof is as shown in Example 1. Preferably, the weight percentage of Rhodiola may be 75 to 90% by weight, and the weight percentage of the Chinese herbal compound may be 10 to 25 wt%.

In the step S11, the organic substance may be an alcohol (for example, ethanol) or the like, and the solvent described in the step S13 may be a common matrix solvent for cosmetics, such as 1,3-butanediol (not limited thereto). The concentration method may include vacuum concentration, vacuum concentration, infrared concentration, freeze dehumidification concentration, hot air concentration, etc., wherein the effect of concentration under reduced pressure is optimal.

The above-mentioned Chinese herbal medicine combination may further include Astragalus, Chuanxiong, Salvia miltiorrhiza or a combination thereof. The method for producing the Rhodiola composition of the present invention may further comprise adding a pharmaceutically acceptable excipient or an additive to the Rhodiola composition of the present invention to prepare the Rhodiola composition of the present invention. A powder form, a granule form, an emulsion form or a liquid form, for example, a dosage form of a tablet, a capsule, a tablet, a powder, an ointment or a spray, which is applied to the skin by a user. The above excipients and additives have been disclosed in Example 1, and therefore will not be further described herein.

Example 3: Antioxidant and free radical scavenging activity of Rhodiola composition of the present invention

In order to confirm that the Rhodiola composition of the present invention has antioxidant and free radical scavenging activities, the following test was conducted. First, the test samples were divided into four groups, as shown in Table 1 below. It should be noted that The ratios of Rhodiola and Chinese herbal medicines in Experimental Group 1 and Experimental Group 2 were 90%, 80%, 10%, and 20%, respectively, and were not limited to this. The composition ratio of Rhodiola and Chinese herbal compound was also It can be any ratio in the range of ratios disclosed in the present invention.

The ratios of the herbal materials in the above two groups were equal. For example, when the total Chinese herbal medicine was 12 g, the control group 1 was 3 g of red peony, mulberry leaves, alfalfa and licorice, while the control group 2 was Rhodiola, Astragalus, and Chuanxiong. And Danshen is 3g each. In addition, the proportions of the Chinese herbal medicines in the herbal group of the experimental group 1 and the experimental group 2 are all equal, that is, in the 10% Chinese herbal compound compound (experiment group 1), the weight percentages of red peony, mulberry leaves, medlar and licorice are respectively 2.5 wt%; In the 20% Chinese herbal compound (experimental group 2), the weight percentages of red peony, mulberry leaf, astragalus, medlar, licorice, chuanxiong and salvia miltiorrhiza were about 2.86 wt%, respectively. The test method for antioxidants in this test includes the ability to remove 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the removal of 2,2- hydrazine. - 2,2-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid, ABTS) free radical capacity test. The main principle of the DPPH free radical scavenging test is that lipids generate free radicals during oxidation, which leads to rancidity. Common antioxidants can remove lipid peroxides by providing hydrogen. From the base, the DPPH is used to evaluate the hydrogen supply capacity of the antioxidant. The DPPH solution has a strong absorbance at 517 nm. The lower the absorbance value, the stronger the hydrogen supply capacity of the antioxidant.

Another, and ABTS peroxidase (peroxidase) and hydrogen peroxide (H2O2) reaction generates ABTS ⁺ ions, this ABTS ⁺ ions is a relatively stable blue-green substance absorption peak at 734nm, anti-oxidants and with ABTS ⁺ The reaction further suppresses the generation of this color, so the lower the absorbed light value, the better the oxidation resistance. The test group was tested at a concentration of 125 μg/ml, and the test results are shown in Table 2 below.

The results of the above Table 2 show that the free radical scavenging effects of the experimental group 1 and the experimental group 2 are better than those of the control group 1 and the control group 2, so the Rhodiola composition of the present invention is excellent for radical scavenging and anti-oxidation. The effect.

Example 4: Whitening test of Rhodiola composition of the present invention

This example is a whitening test by inhibiting tyrosinase. Table 3 below shows the results of tyrosinase inhibition rate, and the test concentration of each group was 500 μg/ml. The KA group contains only Kojic acid, which is a legal whitening ingredient.

As can be seen from the above Table 3, the inhibition rate of the Rhodiola composition of the present invention to tyrosinase can reach 96 to 98%, which is much higher than that of the control group and the KA group. Therefore, the Rhodiola composition of the present invention was further tested at a concentration of 100 μg/ml as shown in Table 4 below.

From the results of the above Table 4, it was found that the Rhodiola composition of the present invention can inhibit tyrosinase at a concentration of 100 μg/ml, and the inhibition rate is 83%.

Example 5: Anti-photoaging test of Rhodiola composition of the present invention

In this embodiment, fibroblasts are irradiated with ultraviolet rays (UVA, 10 J/cm 2 ), and ultraviolet rays stimulate matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-3 (MMP). -3) Ascending, and the tissue inhibitor of metalloproteinases (TIMP) is decreased, resulting in a decrease in collagen and wrinkles. Therefore, if the test sample has the effect of lowering MMP-1 and MMP-3, or increasing TIMP, it may have the potential to resist photoaging.

This example is tested with CCD-966SK fibroblasts, and is not limited thereto. First, the toxicity of each group to CCD-966SK fibroblasts was analyzed by MTT (3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), and the concentration evaluation for non-toxicity of cells was selected. For the inhibition of MMP-1 and MMP-3.

MTT is a yellow compound, a dye that accepts hydrogen ions, which acts on the respiratory chain in the mitochondria of living cells. Under the action of succinate dehydrogenase (SDH) and cytochrome C, the tetrazolium ring is cracked and generated. Blue formazan crystals, the amount of formazan crystals produced is proportional to the number of living cells (single succinate dehydrogenase disappears in dead cells, MTT cannot be reduced). The ability to reduce MTT (formazan formation) can be determined by measuring the 540 nm O.D. value. This O.D. value represents the activity of the mitochondria, that is, the number of viable cells, so the MTT assay can be used as an indicator of cell viability.

The results showed that all extracts were not significantly toxic to fibroblasts at 100 μg/ml, as shown in Table 5 below.

Further, the inhibition of MMP-1 and MMP-3 by each group was analyzed by an enzyme-linked immunosorbent assay (ELISA) method, and the secretion amount of the irradiated UVA cells was taken as 100%, and the results are shown in Table 6 below.

The results in Table 6 above show that the Rhodiola composition of the present invention can inhibit 20 to 40% of MMP-1 at a concentration of 100 μg/ml, and can also lower MMP-3.

Example 6: Bacteriostatic test of Rhodiola composition of the present invention

The strain used in this example was Staphylococcus epidermidis and tested at concentrations of 50000 ppm and 25000 ppm, and the bacteriostatic action of each group is shown in Table 7 below. Among them, the positive control group was 3 mg/ml Triclosan, and the inhibition zone was 28 mm; the negative control group was phosphate buffer solution (PBS), which was not produced by the inhibition zone.

The results in Table 7 above show that the Rhodiola composition of the present invention has a bacteriostatic action against Staphylococcus epidermidis.

Example 7: Moisturizing test of Rhodiola composition of the present invention

In Example 7, a prototype maintenance cream containing 0.05% of Rhodiola composition was applied to the inside of the subject's arm, and the moisturizing effect was evaluated by a multifunctional skin tester. The multi-functional skin tester is produced in Germany and its model number is CK MPA580. The evaluation procedure of the moisturizing effect of Example 7 is as follows: the inner side of the arm of the ten subjects is divided into two areas, and the young type maintenance cream containing 0.05% of Rhodiola composition is applied twice in one area. In another area, a prototype maintenance cream containing 0.05% Rhodiola composition (ie, a blank control group) was not applied; the above procedure lasted for 11 weeks and was fixed after each week to match the student's test (paired Student's T- Test) for statistical analysis comparison.

The results of the moisturizing test of Example 7 are shown in Fig. 2, and Fig. 2 is a graph showing the results of the moisturizing test of the Rhodiola composition of the present invention. As shown in Fig. 2, compared with the blank control group, the skin moisture of the young skin care cream containing 0.05% Rhodiola composition showed a significant upward trend after one week, and after 11 weeks of continuous application, The skin moisture of the tester is more than 30%. Therefore, the Rhodiola composition of the present invention has good moisturizing properties. In summary, the Rhodiola composition of the present invention is effective for scavenging free radicals and having an antioxidant function. Further, in the whitening test, the Rhodiola composition of the present invention is better than the control group 1 and the control group 2, and the tyrosinase inhibition rate can reach 96 to 98%. In addition, the Rhodiola composition of the present invention also has good anti-photoaging, antibacterial and moisturizing effects.

Therefore, since the present invention has a specific Chinese herbal medicine material and is composed of a specific ratio, the use of the Rhodiola composition of the present invention can inhibit skin melanin synthesis, promote skin fairness, and have antioxidant activity. The effect is to delay the aging caused by free radicals or light.

The above is intended to be illustrative only and not limiting. Any equivalent modifications or alterations to the spirit and scope of the invention are intended to be included in the scope of the appended claims.

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Claims (10)

A Rhodiola composition having the functions of brightening white, moisturizing, smoothing fine lines, firming skin and conditioning skin, the Rhodiola composition comprises: a Rhodiola; and a Chinese herbal compound comprising at least Red peony, mulberry leaf, alfalfa and licorice, wherein the Rhodiola and the Chinese herbal compound are extracted with an organic matter or water to obtain a mixed extract, and the mixed extract is concentrated, and the mixed extract is concentrated. The Rhodiola composition is obtained by dissolving in a solvent; wherein the weight percentage of the Rhodiola is 60 to 95% by weight, and the weight percentage of the Chinese herbal compound is 5 to 40% by weight, and the Chinese herbal compound is red The weight percentages of mulberry leaves, alfalfa and licorice are each 1.25% to 30%. The Rhodiola composition according to claim 1, wherein the Chinese herbal compound further comprises astragalus, Chuanxiong, Salvia miltiorrhiza, or a combination thereof, wherein the weights of Astragalus, Chuanxiong, Salvia, Radix Paeoniae, Mulberry, Lycium, and Licorice The percentages are each 0.5% to 30%. The Rhodiola composition according to claim 1, which is in the form of a powder, a granule, an emulsion or a liquid. The Rhodiola composition as described in claim 1 is in the form of a tablet, a capsule, a tablet, a powder, an ointment or a spray. The Rhodiola composition of claim 1, further comprising one of a pharmaceutically acceptable excipient or an additive. The Rhodiola composition according to claim 5, wherein the excipient or the additive is selected from the group consisting of a toner, a fragrance, a preservative, an antioxidant, and a chelate. A combination of a mixture, an isotonic agent, a lubricant, a lozenge adjuvant, a coloring agent, a moisturizing agent, a binding agent, and a pharmaceutically compatible carrier. A method for manufacturing a Rhodiola composition comprises the steps of: extracting a Rhodiola and a Chinese herbal compound by using an organic matter or water to obtain a mixed extract, the Chinese herbal compound comprising at least red peony, mulberry leaves, alfalfa and licorice Concentrating the mixed extract; and dissolving the concentrated extract in a solvent to form the Rhodiola composition; wherein the percentage of the Rhodiola is 60-95% by weight, the Chinese herbal compound The weight percentage is 5~40wt%, and the weight percentage of red peony, mulberry leaf, alfalfa and licorice in the Chinese herbal compound is 1.25%~30%. The method for producing a Rhodiola composition according to claim 7, which further comprises the step of adding a pharmaceutically acceptable excipient or an additive to the composition. The method for producing a Rhodiola composition according to claim 8, wherein the excipient or the additive is selected from the group consisting of a toner, a fragrance, a preservative, an antioxidant, a chelating agent, and the like. A group of lubricants, lozenge adjuvants, colorants, humectants, binders, and pharmaceutically compatible carriers. The method for producing a Rhodiola composition according to claim 7, wherein the organic substance comprises ethanol.