TWI493032B - A preparation method of dry bath material containing probiotics - Google Patents

A preparation method of dry bath material containing probiotics Download PDF

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TWI493032B
TWI493032B TW102141699A TW102141699A TWI493032B TW I493032 B TWI493032 B TW I493032B TW 102141699 A TW102141699 A TW 102141699A TW 102141699 A TW102141699 A TW 102141699A TW I493032 B TWI493032 B TW I493032B
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dry bath
preparing
nutrient
bacteria
preparation
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TW102141699A
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TW201518501A (en
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Univ Cheng Shiu
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Description

含有益生菌之乾式浴材的製備方法Preparation method of dry bath containing beneficial bacteria

本發明是有關於一種浴材製備方法,特別是指一種含有益生菌之乾式浴材的製備方法。The invention relates to a method for preparing a bath material, in particular to a method for preparing a dry bath material containing probiotic bacteria.

沐浴是消除精神壓力及緩和身心緊張的最佳方式,可於沐浴時洗去身上污垢。一般傳統之沐浴方式皆以水浴為主,然而水浴方式只能夠清除皮膚表面之污垢。若是要以泡澡的方式促進血液循環,需藉助外在的加熱方式保持水溫。而隨著能源成本日益提高的今日,利用外在能源進行水溫之維持,已不符合節能減碳的需求。Bathing is the best way to relieve stress and ease tension, and wash away dirt on the body during bathing. The traditional bathing method is mainly water bath, but the water bath method can only remove the dirt on the skin surface. If you want to promote blood circulation by bathing, you need to maintain the water temperature by external heating. With the increasing cost of energy today, the use of external energy sources to maintain water temperature is no longer in line with the need for energy conservation and carbon reduction.

因此,本發明之目的,即在提供一種可自行產熱的含有益生菌之乾式浴材的製備方法。Accordingly, it is an object of the present invention to provide a process for the preparation of a dry bath comprising probiotic bacteria which is self-heating.

於是,本發明含有益生菌之乾式浴材的製備方法,包含一菌液製備步驟,及一混合步驟。該菌液製備步驟是將多種營養源依預定比例配製成一營養液並進行滅菌,再將至少一種菌種加入該營養液中形成一菌液,該菌種是由乳酸桿菌、乳酸球菌,及齒雙歧桿菌中選擇至少一種。該混合步驟是將該菌液以溫水稀釋,並與至少一種木屑所形成之基質混合均勻,而形成一含有益生菌之乾式浴 材。Therefore, the method for preparing a dry bath material containing probiotics of the present invention comprises a preparation step of a bacterial liquid, and a mixing step. The preparation step of the bacterial liquid is to prepare a nutrient solution by a plurality of nutrient sources according to a predetermined ratio and sterilize, and then adding at least one strain to the nutrient solution to form a bacterial liquid, the strain is composed of Lactobacillus and Lactococcus. At least one of Bifidobacterium dentate is selected. The mixing step is to dilute the bacterial liquid with warm water and mix it with the matrix formed by at least one kind of wood chips to form a dry bath containing probiotic bacteria. material.

本發明之功效在於,該乾式浴材中之益生菌透過該基質提供養份,可持續進行發酵作用而產熱,當使用者被該乾式浴材覆蓋時,即可透過前述發酵所產生的熱使體溫上升,促進血液循環,不需再使用額外的加熱能源,以產生節能減碳之功效。The effect of the invention is that the probiotics in the dry bath material provide nutrients through the substrate, and the fermentation can be continued to generate heat, and when the user is covered by the dry bath material, the heat generated by the fermentation can be transmitted. Increases body temperature, promotes blood circulation, and eliminates the need for additional heating energy to produce energy-saving and carbon-reducing effects.

21‧‧‧菌液製備步驟21‧‧‧Bacterial preparation steps

22‧‧‧混合步驟22‧‧‧Mixed steps

3‧‧‧浴池3‧‧‧ bath

31‧‧‧內層31‧‧‧ inner layer

311‧‧‧容置空間311‧‧‧ accommodating space

32‧‧‧保溫層32‧‧‧Insulation

33‧‧‧外層33‧‧‧ outer layer

34‧‧‧蓋子34‧‧‧ cover

341‧‧‧透氣孔341‧‧‧ venting holes

本發明之其他的特徵及功效,將於參照圖式的實施方式中清楚地呈現,其中:圖1是一流程圖,說明本發明含有益生菌之乾式浴材的製備方法之一較佳實施例;圖2是一俯視圖,說明與該較佳實施例配合使用之一浴池的結構;圖3是一剖視圖,輔助說明圖2,為了便於說明,省略部分元件未繪出;及圖4至6皆是曲線圖,說明本發明選用之菌種進行液相層析的結果。Other features and effects of the present invention will be apparent from the following description of the drawings. FIG. 1 is a flow chart illustrating a preferred embodiment of the method for preparing a dry bath containing probiotics of the present invention. Figure 2 is a top plan view showing the structure of a bath used in conjunction with the preferred embodiment; Figure 3 is a cross-sectional view, with assistance in the description of Figure 2, for the sake of convenience, some of the components are not shown; and Figures 4 to 6 It is a graph showing the results of liquid chromatography of the strain selected in the present invention.

參閱圖1,本發明含有益生菌之乾式浴材的製備方法之一較佳實施例,包含一菌液製備步驟21,及一混合步驟22。Referring to Figure 1, a preferred embodiment of a method for preparing a dry bath comprising a probiotic comprises a bacterial liquid preparation step 21 and a mixing step 22.

該菌液製備步驟21是將多種營養源依預定比例配製成一營養液並進行滅菌,再將至少一種菌種加入該營 養液中形成一菌液。該菌種是由乳酸桿菌、乳酸球菌,及齒雙歧桿菌中選擇至少一種,於本實施例中,是選用L.buchneri、L.lactis、及bifidobacterium longum、Streptococcus thermophilus、L.bulgaricus三者之混合菌株進行試驗,當然也可以選用L.amilophylus、L.acidophilus、L.bulgaricus、L.brevis、、Lactobacillus delbrueckii、L.rhamnosus、Streptococcus sp.、L.helveticus、L.plantarum、L.pentosus,或此等之一組合進行試驗,不以本實施例所揭露者為限。另外,該營養液的配製方式是將400至1200公克的營養源、400至1200公克的糖類、20公克的蛋白凍(peptone)共同加入20公升的水中,攪拌溶解均勻,以121℃滅菌20分鐘,降溫到室溫即可使用。該營養源是由麥芽萃取液(malt extract)或酵母粉(yeast)中擇一,而糖類可由蔗糖、果糖,或葡萄糖中擇一。The bacterial liquid preparation step 21 is to prepare a plurality of nutrient sources into a nutrient solution according to a predetermined ratio and sterilize, and then add at least one strain to the camp. A bacterial liquid is formed in the nutrient solution. The strain is selected from at least one of Lactobacillus, Lactobacillus, and Bifidobacterium dentate. In this embodiment, L. buchneri, L. lactis, and bifidobacterium longum, Streptococcus thermophilus, and L. bulgaricus are selected. Mixed strains are tested, of course, L. amilophylus, L. acidophilus, L. bulgaricus, L. brevis, Lactobacillus delbrueckii, L. rhamnosus, Streptococcus sp., L. helveticus, L. plantarum, L. pentosus, or One of these combinations is tested and is not limited to those disclosed in this embodiment. In addition, the nutrient solution is prepared by adding 400 to 1200 g of a nutrient source, 400 to 1200 g of sugar, and 20 g of peptone to 20 liters of water, stirring and dissolving uniformly, and sterilizing at 121 ° C for 20 minutes. It can be used by cooling to room temperature. The nutrient source is selected from a malt extract or a yeast powder, and the sugar may be selected from sucrose, fructose, or glucose.

該混合步驟22是將該菌液以溫水稀釋,並與一基質混合均勻,而形成一含有益生菌之乾式浴材。其中,該基質包含至少一種木屑、五穀雜糧、玉米、木薯、椰子、蔗糖、甜菜根,及豆類之混合物。其中,該木屑可選自於樟木、檜木,或其他木材。於本實施例中,是以200公斤之木屑、60公斤之麥料或玉米粉、60公斤的砂糖或蔗糖、3公斤之豆粉、15公斤之米糠混合而成,但不以此為限。In the mixing step 22, the bacterial liquid is diluted with warm water and uniformly mixed with a substrate to form a dry bath containing probiotic bacteria. Wherein the matrix comprises at least one mixture of wood chips, whole grains, corn, tapioca, coconut, sucrose, beetroot, and beans. Wherein the wood chips may be selected from the group consisting of eucalyptus, eucalyptus, or other wood. In the present embodiment, 200 kilograms of wood chips, 60 kilograms of wheat or corn flour, 60 kilograms of sugar or sucrose, 3 kilograms of soy flour, and 15 kilograms of rice bran are mixed, but not limited thereto.

先將上述菌液34公升以溫水170公升稀釋備用,並將上述基質先置於一如圖2、3所示浴池3中,該浴池3包含一圍繞出一容置空間311之內層31、一設置於該 內層31外側之保溫層32、一設置於該保溫層32外側之外層33,及多個遮蓋該容置空間311的蓋子34,該保溫層32是夾置於該內、外層31、33之間,而上述基質是置於該容置空間311內。其中,該內層31為不銹鋼材質,該外層33則是以樟木、檜木,或其他木材所製成,以產生木頭之香氣並兼顧外觀質感。34 liters of the above-mentioned bacterial solution is diluted with 170 liters of warm water, and the substrate is first placed in a bath 3 as shown in FIG. 2 and 3. The bath 3 includes an inner layer 31 surrounding an accommodating space 311. One set in the An insulating layer 32 on the outer side of the inner layer 31, an outer layer 33 disposed on the outer side of the heat insulating layer 32, and a plurality of covers 34 covering the accommodating space 311. The heat insulating layer 32 is sandwiched between the inner and outer layers 31 and 33. The substrate is placed in the accommodating space 311. The inner layer 31 is made of stainless steel, and the outer layer 33 is made of eucalyptus, eucalyptus, or other wood to produce aroma of wood and a sense of appearance.

接著,將稀釋之菌液淋灑於該浴池3中之基質上,並以人工或攪拌機充分攪拌均勻而形成該乾式浴材,則所述菌種即可以該基質為養份進行發酵作業並產熱,再將所述蓋子34蓋上,以維持該基質之溫度。所述蓋子34上形成有透氣孔341,使該容置空間311內的空氣流通,提供所述菌種足夠的氧氣。一般而言,該浴池3中可維持45℃至60℃的溫熱狀態。Then, the diluted bacterial liquid is sprayed on the substrate in the bath 3, and is sufficiently stirred by a hand or a mixer to form the dry bath material, and the strain can be fermented by the substrate as a nutrient. Heat, the lid 34 is then capped to maintain the temperature of the substrate. A vent hole 341 is formed in the cover 34 to circulate air in the accommodating space 311 to provide sufficient oxygen for the strain. In general, the bath 3 can maintain a warm state of 45 ° C to 60 ° C.

使用時,使用者或坐或臥地位於該浴池3的容置空間311中,可自行或由旁人協助於身上覆蓋該乾式浴材。該乾式浴材中之益生菌透過該基質提供養份,可持續進行發酵作用而產熱,當使用者被該乾式浴材覆蓋時,即可透過前述發酵所產生的熱使體溫上升,促進血液循環,不需再使用額外的加熱能源。再者,將本實施例所選用之菌株以前述營養液培養後,以液相層析串聯質譜儀(LC/MS/MS)進行乳酸成分之分析,如圖4至圖6所示,各圖式中左側之波峰即為左旋乳酸(L-form Lactic acid),右側之波峰即為右旋乳酸(D-form Lactic acid),而於本實施例所選用之菌株,皆能產生高濃度之左旋乳酸的成分(圖4為L. buchneri、圖5為L.lactis、圖6為bifidobacterium longum、Streptococcus thermophilus、L.bulgaricus三者之混合菌株)。而左旋乳酸已知可達到去除老舊角質層、使皮膚細潤等功效,因此本發明除了使讓使用者的體溫升高,促進血液循環之外,亦能使肌膚達到光滑、提升彈性等效果。When in use, the user can sit or lie in the accommodating space 311 of the bath 3, and can cover the dry bath material by himself or by others. The probiotics in the dry bath material provide nutrients through the substrate, and the fermentation can be continued to generate heat. When the user is covered by the dry bath material, the heat generated by the fermentation can be used to raise the body temperature and promote blood. Cycle, no need to use additional heating energy. Furthermore, after the strain selected in the present embodiment is cultured in the nutrient solution, the lactic acid component is analyzed by liquid chromatography tandem mass spectrometry (LC/MS/MS), as shown in FIG. 4 to FIG. In the formula, the peak on the left side is L-form Lactic acid, and the peak on the right side is D-form Lactic acid, and the strain selected in this embodiment can produce high concentration of left-handed rotation. The composition of lactic acid (Figure 4 is L. Buckneri, Figure 5 is L. lactis, Figure 6 is a mixed strain of bifidobacterium longum, Streptococcus thermophilus, L. bulgaricus). L-lactic acid is known to have the effect of removing the old stratum corneum and making the skin fine. Therefore, the present invention not only allows the user's body temperature to rise, but also promotes blood circulation, and also enables the skin to achieve smoothness and elasticity.

綜上所述,本發明含有益生菌之乾式浴材的製備方法,透過該基質提供益生菌所需養份,可持續進行發酵作用而產熱,當使用者被該乾式浴材覆蓋時,不需再使用額外的加熱能源,即可使體溫上升,促進血液循環,具有節能減碳之功效,且所述益生菌所產生之左旋乳酸,亦有促進角質代謝,而使皮膚細潤、光滑、提升彈性,故確實能達成本發明之目的。In summary, the method for preparing a dry bath material containing probiotic bacteria provides a nutrient for the probiotic bacteria through the substrate, and the fermentation is continued to generate heat, and when the user is covered by the dry bath material, The additional heating energy is needed to increase the body temperature, promote blood circulation, and have the effect of energy saving and carbon reduction, and the L-lactic acid produced by the probiotic bacteria also promotes keratin metabolism, and makes the skin fine, smooth and improved. It is elastic, so it can achieve the object of the present invention.

惟以上所述者,僅為本發明之較佳實施例而已,當不能以此限定本發明實施之範圍,即大凡依本發明申請專利範圍及專利說明書內容所作之簡單的等效變化與修飾,皆仍屬本發明專利涵蓋之範圍內。The above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, that is, the simple equivalent changes and modifications made by the patent application scope and patent specification content of the present invention, All remain within the scope of the invention patent.

21‧‧‧菌液製備步驟21‧‧‧Bacterial preparation steps

22‧‧‧混合步驟22‧‧‧Mixed steps

Claims (3)

一種含有益生菌之乾式浴材的製備方法,包含:一菌液製備步驟,將多種營養源依預定比例配製成一營養液並進行滅菌,再將多種菌種加入該營養液中形成一菌液,該等菌種是乳酸桿菌、乳酸球菌,及齒雙歧桿菌之組合,藉以產生左旋乳酸,其中,該等菌種是選自於L.amilophylus、L.acidophilus、L.bulgaricus、L.brevis、L.buchneri、L.casei、Lactobacillus delbrueckii、L.rhamnosus、L.lactis、Streptococcus sp.、Streptococcus thermophilus、L.helveticus、L.plantarum、L.pentosus、bifidobacterium longum;及一混合步驟,將該菌液以溫水稀釋,並與至少一種木屑所形成之基質混合均勻,而形成一含有益生菌之乾式浴材。 The invention discloses a preparation method of a dry bath material containing probiotics, comprising: a preparation step of preparing a bacterial liquid, preparing a plurality of nutrient sources into a nutrient solution according to a predetermined ratio and sterilizing, and adding a plurality of strains to the nutrient solution to form a bacteria; a liquid, the bacteria are a combination of Lactobacillus, Lactobacillus, and Bifidobacterium breve, thereby producing L-lactic acid, wherein the bacteria are selected from the group consisting of L. amilophylus, L. acidophilus, L. bulgaricus, L. Brevis, L. buchneri, L. casei, Lactobacillus delbrueckii, L. rhamnosus, L. lactis, Streptococcus sp., Streptococcus thermophilus, L. helveticus, L. plantarum, L. pendos, bifidobacterium longum; and a mixing step The bacterial solution is diluted with warm water and uniformly mixed with a matrix formed by at least one kind of wood chips to form a dry bath material containing probiotic bacteria. 如請求項1所述含有益生菌之乾式浴材的製備方法,其中,該營養液的配製方式是將400至1200公克的營養源、400至1200公克的糖類、20公克的蛋白凍共同加入20公升的水中,該營養源是由麥芽萃取液或酵母粉中擇一。 The method for preparing a dry bath containing probiotics according to claim 1, wherein the nutrient solution is prepared by adding 400 to 1200 g of a nutrient source, 400 to 1200 g of sugar, and 20 g of protein jelly together. In liters of water, the nutrient source is selected from malt extract or yeast powder. 如請求項1所述含有益生菌之乾式浴材的製備方法,其中,該混合步驟中之基質還包含五穀雜糧、玉米、木薯、椰子、蔗糖、甜菜根,及豆類之混合物。 The method for preparing a dry bath containing probiotics according to claim 1, wherein the matrix in the mixing step further comprises a mixture of whole grains, corn, cassava, coconut, sucrose, beetroot, and beans.
TW102141699A 2013-11-15 2013-11-15 A preparation method of dry bath material containing probiotics TWI493032B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63179829A (en) * 1987-01-21 1988-07-23 Advance Co Ltd Antibacterial agent for skin
JP2006089474A (en) * 2004-08-27 2006-04-06 Hisakazu Ikeda Herbaceous material-fermenting bath

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63179829A (en) * 1987-01-21 1988-07-23 Advance Co Ltd Antibacterial agent for skin
JP2006089474A (en) * 2004-08-27 2006-04-06 Hisakazu Ikeda Herbaceous material-fermenting bath

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