TWI425207B - Biochemical test strip - Google Patents

Description

Biochemical test strip

The invention relates to a biochemical test piece, relating to a biochemical test piece which utilizes a buffer groove design of a flow channel to increase the detection accuracy.

With the advancement of medicine and the increasing health concept of modern people, in the past only the physical condition test provided by the hospital was available. Nowadays, it has been improved at home and can be self-tested, for example: blood glucose machine, so the operation is simple, the volume is small, and the measurement speed is fast, which is the advantage of the current detection machine.

In order to make the detector reach the requirement of miniaturization of the sampling amount, the volume of the biochemical test piece is also shrinking. When the sample is taken, it is affected by the user's operating habits. Users who are not familiar with the operation process are easy to make the test. The body (for example, blood) does not have a sufficient volume to be spread on the electrode substrate of the biochemical test piece, causing an excessive error in the detection result and reducing the accuracy.

An object of an embodiment of the present invention is to provide a biochemical test piece which can increase the measurement accuracy or reduce the error of the detection result.

It is an object of an embodiment of the present invention to provide a biochemical test strip that allows a sample to have a sufficient volume to be spread over two working electrodes.

An embodiment of the invention provides a biochemical test strip comprising a first-class channel, the flow channel comprising a collection port and a buffer tank. The collection port is used for a sample to flow into the flow channel; the buffer groove is used for the sample to flow in and store the sample.

According to an embodiment of the invention, the flow channel is provided with a buffer tank, and the sample body flows in the flow channel by capillary phenomenon, and a part is stored in the buffer groove of the flow channel, so as to avoid the measurement of the sample with too small volume, and the error is generated. The accuracy of the test.

Please refer to FIG. 1 and FIG. 2 simultaneously. FIG. 1 is a schematic view showing the appearance of an embodiment of the biochemical test piece of the present invention, and FIG. 2 is a detailed view of the biochemical test piece 100 of FIG. The biochemical test strip 100 includes an electrode substrate 101, a flow path plate 102, and a top plate 103.

The biochemical test strip 100 has a first-class track 105, and the flow path 105 has a collection port 10 and an opening 11. The user drops the specimen into the collection port 10 to detect the specimen using the biochemical test strip 100. It should be noted that the position of the collection port 10 can be adjusted according to different needs. In the present embodiment, the position of the collection port 10 is set at the front end of the biochemical test strip 100, and for simplicity of description, the sample is exemplified by blood, but the invention should not be limited thereto, and may also be a human body. Other body fluids, such as saliva or urine.

The flow path plate 102 is disposed between the electrode substrate 101 and the top plate 103, and the electrode substrate 101, the flow path plate 102, and the top plate 103 collectively define the flow path 105 such that blood flows in the flow path 105 due to capillary action. More specifically, the flow channel plate 102 is disposed on the upper layer of the electrode substrate 101 and has a channel 104. The top plate 103 is disposed on the upper layer of the flow channel plate 102. In the present embodiment, the bottom-up order of the biochemical test strips 100 is the electrode substrate 101, the flow channel plate 102, and the top plate 103, wherein the top plate 103 is used to form a flow with the electrode substrate 101 and the flow channel plate 102. The passage 105, more specifically, causes the passage 104 of the flow path plate 102 to be sandwiched between the electrode substrate 101 and the top plate 103 to form the flow path 105.

In order to allow the blood to flow more smoothly, an opening 11 may be formed at the position of the top plate 103 corresponding to the passage 104 of the flow path plate 102 for exhausting. In other words, the biochemical test strip 100 as a whole, the opening 11 is like a vent of the flow path 105 for discharging the air flowing in the flow path 105, thereby increasing the flow of blood in the flow path 105. Sex. Additionally, in an embodiment of the invention, the location of the opening 11 is disposed above the channel 104.

Next, please refer to FIG. 3 and FIG. 4A simultaneously. FIG. 3 shows a schematic diagram of an embodiment of the flow channel plate 102, and FIG. 4A shows a schematic diagram of blood flow in the flow channel 105. The flow path 105 has buffer grooves 12 on both sides for allowing blood to flow in and store. In an embodiment, the buffer groove 12 is triangular in shape and the number is two, but the buffer groove 12 in the present invention is changed in the nature of the specimen, so the buffer groove 12 can be any shape (for example, semicircular, Square). In the present embodiment, two notches can be formed at the channel 104 of the flow channel plate 102, and the flow channel buffer grooves 12 are formed because the two notches are sandwiched between the electrode substrate 101 and the top plate 103.

In the present embodiment, since the extending direction of the buffer groove 12 (the direction D1 in the drawing) is different from the direction of the flow path 105 (the direction D2 in the drawing), an angle ψ is formed between the two directions. Therefore, when blood flows in the flow path 105 and flows through the buffer tank 12, a part of the blood continues to flow in the direction of the flow path 105 (direction D2 in the drawing), and another part of the blood flows into the buffer tank 12. (D1 direction in the figure). Preferably, the structure of the flow passage 105 having the buffer tank 12 enables a smaller portion of the blood to continue to flow along the flow passage 105, and a larger portion of the blood flows into the buffer tank 12. Therefore, in order to make blood flow more easily into the buffer tank 12, the angle ψ is designed to be less than 90 degrees.

In addition, in an embodiment, the flow channel 105 may also be curved. Fig. 4B is an enlarged view of a region in which the blood of Fig. 4A passes through the flow path 105 corresponding to the buffer tank 12. Referring to FIG. 4B, the blood line passes through a portion of the flow path 105 corresponding to the flow channel 105 of the buffer groove 102, such as the S region, in a first direction D2, and the buffer groove 102 is in a second direction from the portion of the flow channel 105. D1 extends, and the angle between the first direction D1 and the second direction D2 is not more than 90 degrees.

Please refer to FIG. 5, which shows a schematic view of the electrode substrate 101 of the present embodiment. The electrode substrate 101 has a plurality of electrodes. In one embodiment, the electrode substrate 101 includes the working electrodes 101a, 101b and the reference electrode 101c, wherein the reference electrode 101c and the working electrode 101a have different voltage polarities; and the reference electrode 101c and the working electrode 101b have different voltage polarities, such that the working electrode 101a and the reference electrode 101c form a voltage difference; the working electrode 101a and the working electrode 101b form another voltage difference. Any conductive material can be the material of the electrode. In this embodiment, graphite can be used, but the invention should not be limited thereto.

Please refer to FIG. 6. FIG. 6 is a perspective view showing the biochemical test strip 100 of the present invention. The working electrodes 101a and 101b and the reference electrode 101c are disposed under the flow channel 105. When the user inserts the biochemical test strip 100 into a detecting machine (not shown), for example, a blood glucose meter, and drops blood into the collecting port 10, the blood first flows through the flow path 105 through the reference electrode 101c, and then flows through The working electrode 101b then flows through the working electrode 101a.

In one embodiment, the working electrode 101a and the reference electrode 101c are used to detect the amount of blood. When the blood flows from the reference electrode 101c to the working electrode 101a, since the voltages of the working electrode 101a and the reference electrode 101c are opposite in polarity, a Loop and start the tester for measurement. Further, since a voltage difference is formed between the working electrodes 101a and 101b, the detecting device measures the measured value of the blood using the voltage difference between the working electrodes 101a and 101b, so that the working electrodes 101a and 101b are used to measure a measured value at this time. Therefore, when the blood first passes through the reference electrode 101c, it is determined that there is blood inflow with the detecting machine, and when the blood flows to the working electrode 101a, the amount of the required measurement is determined with the detecting machine, and then the working electrodes 101a and 101b measure the measurement by using the voltage difference. Value, in this embodiment the measured value is a blood glucose value.

Please refer to FIG. 7A and FIG. 7B at the same time. FIG. 7A and FIG. 7B are schematic diagrams of blood distribution after operation of a conventional technique in the case of insufficient blood. Since there is no buffer tank 12 design, blood has a chance to be distributed on the working electrode 101a. The volume of 101b is too small, but even if the blood volume of the working electrodes 101a and 101b is too small, the working electrode 101a and the reference electrode 101 form a loop, and the detector starts and measures a measured value. At this time, since the blood volume distributed in the working electrodes 101a and 101b is too small, there is a large error value in the measured value at this time.

In general, the amount of blood that can be stored in the flow channel 105 is 0.6μ. Please refer to Figure 7C. Figure 7C is a schematic diagram of blood distribution after operation in the absence of blood. When the user has a small amount of blood dripping into the collection port 10 due to physiological conditions or operational errors (for example, the user provides only 0.3 blood volume) ), part of the blood enters the buffer tank 12, so that the working electrode 101a and the reference electrode 101c cannot form a loop. At this time, the detector cannot measure the measured value by the working electrodes 101a and 101b. In this way, the measurement of the error too much can be further avoided by avoiding insufficient blood volume.

In one embodiment, the volume of the buffer tank 12 can be set to be smaller than the volume of the flow channel 105. However, the present invention should not be limited thereto, and the amount of stored blood of the buffer tank 12 can be adjusted according to the requirements of different detectors.

In summary, the biochemical test strip of the embodiment of the present invention utilizes the design of the buffer tank on the flow channel to store the sample in the buffer tank through capillary phenomenon, so that the electrode of the lower layer of the flow channel has sufficient volume of the sample for detection. To improve the shortcomings of the prior art, so that the detector can provide accurate measurement values and reduce the error rate under sufficient volume of the sample.

100. . . Biochemical test strip

10. . . Collection port

11. . . Opening

12. . . Buffer tank

101. . . Electrode substrate

101a, 101b. . . Working electrode

101c. . . Reference electrode

102. . . Flow channel board

103. . . roof

104. . . aisle

105. . . Runner

Fig. 1 is a view showing the appearance of an embodiment of a biochemical test piece.

Fig. 2 is a view showing a detailed diagram of the biochemical test piece of Fig. 1.

Figure 3 shows a schematic diagram of one embodiment of a flow channel plate.

Figure 4A shows a schematic representation of the flow direction of blood in the flow channel.

Fig. 4B is an enlarged view of a region in which the blood of Fig. 4A passes through the position corresponding to the buffer tank in the flow path.

Fig. 5 is a view showing an electrode substrate of an embodiment.

Figure 6 shows a perspective view of the biochemical test strip.

Figure 7A is a schematic diagram of blood distribution after operation of a conventional technique in the absence of blood.

Figure 7B is a schematic diagram of blood distribution after operation of a conventional technique in the absence of blood.

Fig. 7C is a schematic view showing the distribution of blood after operation in the case of insufficient blood in the present invention.

100. . . Biochemical test strip

10. . . Collection port

11. . . Opening

12. . . Buffer tank

101. . . Electrode substrate

101a, 101b. . . Working electrode

101c. . . Reference electrode

105. . . Runner

Claims (15)

A biochemical test piece comprising: a first-class track, the flow channel comprising: a collection port for a sample to flow into the flow channel; and a buffer groove for the sample to flow into and store the sample; a first working electrode is disposed on the flow channel; and a second working electrode is disposed on the flow channel, such that the sample flows through the first working electrode and then flows through the second working electrode, wherein the buffer groove Located between the collection port and the second working electrode. The biochemical sensing test piece according to claim 1, wherein the volume of the buffer tank is smaller than the volume of the flow channel, and the inspection system passes through a position in the flow channel corresponding to a portion of the buffer groove in a first direction, and the The buffering groove extends from the portion of the flow channel in a second direction, and the angle between the first direction and the second direction is no more than 90 degrees. The biochemical test strip according to claim 1, wherein the buffer tank is located between the first working electrode and the second working electrode. The biochemical sensing test piece according to claim 1, wherein the inspection system passes through a position in the flow path in a first direction corresponding to a portion of the buffer groove, and the buffer groove is from the portion of the flow path. The two directions extend, and the angle between the first direction and the second direction is not more than 90 degrees. The biochemical test piece according to claim 1, wherein the buffer tank has a volume smaller than a volume of the flow channel. The biochemical sensing test piece according to claim 1, wherein the flow channel further comprises a vent, and the second working electrode is located between the collecting port and the vent. The biochemical sensing test piece according to claim 1, wherein the detecting body flows between the first working electrode and the second working electrode, and a detecting machine forms between the first working electrode and the second working electrode. A voltage difference is used to measure a measured value of the sample using the voltage difference. The biochemical test strip according to the first aspect of the invention, wherein the biochemical test strip comprises: an electrode substrate, the first working electrode and the second working electrode; a first-class track plate having a channel; and a a top plate; wherein the flow channel plate is disposed between the top plate and the electrode substrate, and the top plate, the flow channel plate and the electrode substrate define the flow channel according to the channel. The biochemical test strip according to claim 8, wherein the top plate defines an opening corresponding to the passage of the flow passage plate to form a vent of the flow passage. The biochemical sensing test piece according to claim 1, further comprising a reference electrode disposed in the flow channel, wherein the first working electrode is located between the reference electrode and the second working electrode position, so that the sample flow After passing through the reference electrode and the first working electrode, the second working electrode is passed through. The biochemical sensing test piece according to claim 10, wherein the reference electrode and the first working electrode have different voltage polarities; and the reference electrode and the second working electrode have different voltage polarities. The biochemical sensing test piece described in claim 1 is made of a conductive material. The biochemical sensing test pieces described in claim 12, wherein the electrodes are made of graphite. A biochemical test piece according to claim 7, wherein the sample is blood. The biochemical test piece described in claim 14 of the patent application, wherein the measured value is a blood sugar level.