TWI302604B - Electrochemical test strip,electrochemical sensing system,and method of using the same - Google Patents

Description

1302604 IX. Description of the Invention: [Technical Field] The present invention relates to an electrochemical test strip, in particular, to start the instrument, confirm the correct position of the test paper into the instrument, identify the reaction zone of the sample, and properly cover the sample. Electrochemical test of the reaction zone. [Prior Art] The use of electrochemical test strips has been a common technique in medical or biochemical testing. The conventional electrochemical test paper has two electrodes, and after the product is injected into the reaction zone of the test paper, the sample characteristics are measured by the two (four) poles. However, under such a structure, it is impossible to carry out the measurement and solve the above problem, and the US Patent No. 558 is added to the third electrode, which is the farthest from the population of the other two electric regions. After the injection of the sample, the ί ^ ^ reaction area takes the near population and the two farthest from the population. (4) Whether the sample is properly covered by the two electrodes outside the third electrode, into the measuring instrument and established with the instrument. After the electrical connection, however, the square cover disclosed in the U.S. Patent No. 5,582,697, the majority of the slabs, must be enchanted to the reaction zone of the sample to confirm that the sample has entered the reaction zone. And can provide a kind of chemical test paper that can be placed in the instrument state. 4APEX05002TW.doc 1302604 SUMMARY OF THE INVENTION In the prior art, the present invention provides an electrochemical test paper for assisting the placement of the test paper in the correct position of the instrument and for identifying whether the sample enters the reaction zone and enters the reaction zone. Is the sample sufficient?

According to an aspect of the invention, an electrochemical test strip for detecting the function of injecting a sample is provided. The electrochemical test paper comprises an insulating substrate; an electrode system disposed on the insulating substrate, the electrode system comprises an insulating electrode, a reference electrode, a working electrode and a detecting electrode, and the detecting electrode and the reference electrode Connected through a resistor; and an insulating layer placed over the electrode system, wherein a portion of the electrode system not covered by the insulating layer forms a reaction region having a - open end. When the liquid sample is injected into the reaction zone from the open end, the identification electrode, the reference electrode, the working electrode and the detection electrode are sequentially contacted. An additional aspect of the invention provides an electrochemical sensing system comprising the electrochemical test paper described above and a measuring device. Wherein, the quantity = device comprises a switch module integrated into the electrode system for selectively V-passing or disconnecting the electrode system and the measuring device

ϋ ϋ ' engage 5 to analog converter and switch module, and used to control the opening, j,; and: insert the inductor, coupled to the switch module and process the electricity between the electrochemical test strip and the measuring device Sexual connection.

Aspects provide a method of using the above-described electrochemical sample. The yarn contains the following steps in the I friri placement device, by identifying the electrodes and electricity.

Ϊ́ί二考, the bungee and the measuring device form a loop, rf?3 starts the measuring device'(b)l Measure the impedance value between the identification electrode and the reference electrode to judge J 4APEX05002TW.doc 1302604, the sample is in response In (d), before and after adding the liquid secret, identify the corresponding current. The purpose, embodiments, features, and advantages of the preferred embodiment of the fp of the voltage are determined.

Embodiments The present invention discloses an electrochemical test paper and a method of using the same. In order to make the description of the present invention more detailed and complete, reference is made to the following description in conjunction with the drawings of Figures 1 to 4. The apparatus, elements, and program steps described in the following examples are merely illustrative of the invention and are not intended to limit the scope of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a structural exploded view of an electrochemical test strip in accordance with one embodiment of the present invention, wherein the outline of the electrochemical test strip is represented by dashed lines for ease of understanding the relative position between the elements. The electrochemical test paper of the present invention comprises an insulating substrate 10, an electrode system 20, an insulating layer 40, a hydrophilic spacer 60 and an upper spacer 62. The insulating substrate 10 is electrically insulating, and the material thereof may include, but is not limited to, polyvinyl chloride (PVC), glass fiber, polycrystalline silicon, electric wood, polyethylene terephthalate (pET), polycarbonate ( Pc), polypropylene (PP), polyethylene (PE), polystyrene (PS) or ceramics. The electrode system 20 includes an identification electrode 22, a reference electrode 24, a working electrode 26 and a detection electrode 28 which are insulated from each other, wherein the identification electrode 22 and the reference electrode 24 are connected via a resistor 29. The material of the electrode system 20 may be any conductive material such as carbon glue, silver glue, copper glue, carbon silver mixed glue, or the like 4APEX05002TW.doc 7 1302604 » and combinations thereof. The insulating layer 40 covers a portion of the electrode system 20 such that one end of the uncovered electrode system 20 forms a reaction zone 30 for containing a liquid sample, and the other end forms a connection zone 34 for interfacing with the measuring instrument. The reaction zone 30 has an inlet 32 for the injection of a liquid sample. The material of the insulating layer 40 may include, but is not limited to, pVc insulating tape, pet insulating tape, thermal drying type insulating varnish or ultraviolet drying type insulating varnish. The entrance 32 from the reaction zone 30 is directed inwardly by the identification electrode 22, the reference electrode 24, the working electrode 26 and the detecting electrode 28 in the electrode system 2A. The electrochemical test paper of the present invention further comprises a reaction layer 5〇 disposed in the reaction zone 30, which comprises at least an oxidoreductase for chemical reaction with the liquid of the sample, wherein the redox species vary depending on the liquid sample. Different. The reaction layer 5 〇 covers at least the reference electrode 24 and the working electrode 26 in the reaction zone 30, but may also cover the identification electrode 22 or the detecting electrode 28 in the reaction zone 3A. The hydrophilic separator 6〇 and the upper spacer 62 are combined to form a pore 64 for discharging the gas in the reaction zone 3 to enhance the capillary action to accelerate the injection of the liquid sample, and shorten the liquid sample to fill the reaction zone 30. time. The hydrophilic separator 60 directs the flow of the liquid sample and directs the function of the gas. After the electrochemical test paper is placed in a measuring instrument, the connection through the resistor 29 is such that a circuit is formed between the identification electrode 22, the reference electrode 24 and the measuring instrument, and the measuring instrument is started. After the measuring instrument is started, the impedance value of the identification electrode 22 and the reference impurity (5) can be further measured, and the resistance value of the reducing resistor 29 is compared, and it is judged whether the electrochemical test paper is placed in the correct position. After the liquid sample is injected into the reaction zone of the electrochemical test strip 3, the impedance change between the electrode 22 and the reference electrode 24 is caused, so that the impedance change can be checked to determine whether the liquid sample has entered the reaction zone. In the reaction zone 30, since the identification electrode 22, the reference electrode %, the work 4APEX05002TW.doc 8 1302604, the arrangement of the detection electrode 28 is sequentially from the reaction zone entrance 32 to the end of the cutting pole 28 must be completed. The reference impurity μ is used as the electrode 26°, so 'recognize the sample entering the reaction", and then the $=匕= between the identification electrode 22 and the detecting electrode 28 is judged whether the sample reaches the _ electrode 28. The inspection side = reach the fine electrode 28 ' to judge that the liquid sample has been covered

Si ϊϊί, between the identification electrode 22 and the detection electrode 28, and observed ^. How many 疋 疋 合 样品 样品 样品 样品 样品 到达 • • 』 』 』 』 』 』 』 』 』 』 』 』 』 』 』 』 』 实施 实施 实施 实施 实施 品 品 品 品 品 品 品 品 品 品 品 ί 品 品 品28 t itf Qiu I made electrode 26 and _ electrode 28

= Electrically, Wb' can be used to complete the reference cover 24 and the working electrode 26. N After confirming that the sample is properly covered in the reaction zone for 3 ,, it can be electrically measured between the 24 胄 working electrode 26 of the test electrode, which is mainly measured in the reaction zone 30, in the sample and the reaction layer % The change in current produced by the oxidoreductase action. 2A-2C illustrate three different electrode arrangements and reaction zone shapes formed on an electrochemical test strip, wherein the identification electrode 22, the reference electrode working electrode 26, and the detecting electrode 28 are insulated from each other, and the identification electrode 22 and the reference electrode are 24 is connected through a resistor 29. In Fig. 2A, the reaction zone inlet 32 is directly below. In Fig. 2B, the reaction zone inlet 32 is on the left side and in Fig. 2C, the reaction zone inlet 32 is on the lower left side. As shown in Figures 2A-2C, from the inlet 32 of the reaction £30, the identification electrode 4APEX05002TW.doc 1302604 pole 22, the reference electrode 24, the working electrode 26 and the detection electrode 28 are sequentially contacted. In fact, as long as the arrangement of the four electrodes in the reaction zone 30 is as described above, and the electrodes are insulated from each other, the present invention does not limit the arrangement of the electrodes on the test paper and the shape of the reaction zone 30. 3 is a block diagram of a sensing system 1A according to an embodiment of the invention, the system comprising an electrochemical test strip 200 of the present invention and a measuring device 300. The measuring device 300 includes a connector 320 for external connection, a switch module 32, an amplifier 322, an analog-digital converter 323, a test strip insertion sensor 324, and a processor 325. The identification electrode 222, the reference electrode 224, the working electrode 226, and the detecting electrode 228 of the electrochemical test strip 200 are electrically connected to the measuring device 300 through the interface with the connector 320. The switch module 321 is for selectively connecting the electrodes in the chemical test strip 200 to the ground of the measuring device 3 or to the respective circuits in the measuring device 300. The measuring device 3 further includes a display 326 for displaying various measurements, and a power source 330 for providing the required power. In another embodiment, display 326 and power source 330 can be external devices and are not included in measurement device 30. • When the electrochemical strip 200 is connected to the connector 320, the identification electrode 222, the resistor 229 and the reference electrode 224, the connector 320, the switch module 321 and the insertion inductor 324 form a loop, and the processor 325 is started to measure. Device 300. The insertion sensor 324 can be a conventional power-on circuit that is an open circuit before the test strip 200 is connected and forms a loop after the test strip 200 is attached. For example, the insertion inductor 324 may include a diode or a transistor, and after the test strip 200 is inserted, the diode or transistor that is not turned on is turned on, thereby causing voltage of the diode or the transistor terminal. The change, in turn, activates the measuring device 300. However, in some cases, the activation of the measurement device 300 may be caused by other unintended factors 4APEX05002TW.doc 1302604, so after the measurement device 300 is activated, the processor 325 can provide a voltage to the identification electrode. The impedance value is measured between the 222 and the reference electrode 224, and compared with the impedance value of the resistor 229, it is judged whether or not the electrochemical test strip 200 is correctly connected to the connector 320. In the case where the electrochemical test paper 200 is confirmed to be green-green, the sample is injected into the reaction zone of the test paper 200, and the impedance value between the identification electrode 222 and the reference electrode 224 is changed. This change is digitized by amplifier 322 and analog-to-digital converter 323 and passed to processor 325, allowing processor 325 to confirm that the sample is being dropped into the reaction zone. After confirming that the sample enters the reaction zone, the distribution of the sample in the reaction zone can be further detected. According to an embodiment of the present invention, the processor 32 provides a voltage between the identification electrode 222 and the detection electrode 228. Once the sample reaches the detection electrode 228 from the identification electrode 222 to the bottom of the reaction area, the processor 325 can detect To a sufficient current change, confirm that the sample properly covers the anti-amp; area. According to another embodiment of the present invention, the processor 325 provides a voltage between the working electrode 226 and the detecting electrode 228. Once the sample reaches the detecting electrode 228 at the bottom of the reaction zone, the working electrode 226 and the detecting electrode 228 can be interposed. There is enough current to circulate, and it is confirmed that the product properly covers the reaction zone. After confirming that the sample properly covers the reaction zone (ie, the sample completely covers the reference electrode 224 and the working electrode 226), the processor 325 applies a voltage between the reference electrode 224 and the working electrode 226, and then passes through the amplifier 322 and compares the digital conversion. The device 323 measures the change in current between the reference electrode 224 and the working electrode 226 due to the electrochemical action of the sample. . Fig. 4 is a flow chart showing the flow of a liquid sample using the electrochemical test paper of the present invention. First, the electrochemical test paper of the present invention is provided in steps. As mentioned above, this test strip contains 4 electrodes insulated from each other: Identification 4APEX05002TW.doc 11 1302604

The electrode and the detecting electrode, wherein the identifying electrode is connected to the ίί: pole. Next, in step S4H), the meter is activated to activate the measuring instrument. The instrument starts J 'in step S420, measures the impedance value between the identification electrode and the reference electrode, and the right value is combined with the expected value, and then proceeds to step S43〇 to provide the liquid-liquid product in the electrochemical test paper-inverse Ship. Next, in step S440, the change in the impedance value between the identification electrode and the reference electrode is measured, and if it is expected, the secret sample has been positively injected into the anti-ship. After reading the correct response of the liquid sample, it is optional to proceed to step s45 or step S455. In step S450, the electrical changes of the identification electrode and the detection electrode are measured. If the job is combined, it indicates that the secret sample has adequate coverage of the reverse region. In step S455, the electrical conductivity of the working electrode and the die electrode is measured, and if it is expected, it indicates that the liquid sample has sufficiently covered the reaction zone. Next, in step S·, a change in the reference electrode is measured to detect the characteristics of the liquid sample. The above is only the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention; all other equivalent changes or modifications that do not depart from the spirit of the present invention include Within the scope of the ^& patent. θ [Simple Description of the Drawings] FIG. 1 is an exploded view of an electrochemical test paper according to an embodiment of the present invention; m FIGS. 2A-2C illustrate three different electrode arrangements and reaction zone shapes formed on an electrochemical test paper. FIG. 3 is a sensing system side 4APEX05002TW.doc 12 1302604 according to an embodiment of the invention.

The block diagram, and Figure 4, shows a flow chart of a method for sensing a liquid sample using the electrochemical test strip of the present invention. [Description of main component symbols] 10. Substrate 20, electrode system 22, identification electrode 24, reference electrode 26, working electrode 28, detecting electrode 29, resistor 30, reaction zone 32, inlet 34, connection region 50, reaction layer 60, Hydrophilic septum 62, upper septum 64, air vent 100, sensing system 200, electrochemical test strip 300, measuring device 320, connector 321, 321 module 322, amplifier 323, analog to digital converter 324, insertion sensing 325, processor 330, power supply 326, display 4APEX05002TW.doc 13

Claims (1)

1302604 X. Patent application scope: 1. An electrochemical test paper for detecting the injection state of a sample, comprising: · an insulating substrate; , • and square (four), on the top of the set of the Puji County plate, the mystery contains An acquaintance electrode, a reference electrode, an I-electrode, and an electrode, wherein the identification electrode and the reference electrode are connected to a portion of the electrode system through a resistor, and the portion of the electrode system is not covered by the insulation The _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Electrode and the side electrode. 2. The electrochemistry according to claim i, above the edge layer, the upper cover and the right ^, η ( (4) absolutely have a hole for discharging the gas in the reaction zone 3. As described in claim 1 The electrochemical test strip comprises a reaction zone in which the reaction layer comprises an oxygen-containing reaction. Wherein the reaction layer is at least covered by 4APEX05002TW.doc 1302604. 5. An electrochemical sensing system comprising: one of the electrochemical test strips of claim 1; and a measuring device comprising: a switch module, coupled To the electrode system for selectively turning on or off the electrical connection between the electrode system and the measuring device; an analog-to-digital converter coupled to the switch module; a processor coupled to The analog-to-digital converter and the switch module are used to control the switch module; and an insertion sensor is coupled to the switch module and the processor for detecting the electrochemical test strip and the measuring device Electrical connection between the two. 6. The electrochemical sensing system of claim 5, wherein an electrical change between the identification electrode and the reference electrode is caused before and after the liquid sample is injected into the reaction zone of the electrochemical test strip, It is transmitted to the processor through the analog-to-digital converter. The electrochemical sensing system of claim 5, wherein after the liquid sample is injected into the reaction zone of the far electrochemical test strip, the processor applies a voltage between the identification electrode and the detection electrode. A current change between the identification electrode and the detection electrode is detected. , 4APEX05002TW.doc 15 1302604 8 · The electrochemical sensing system, wherein after the liquid sample is applied to the reaction zone, the processor applies a voltage to the electrical electrode to measure the working electrode and the detection 9· In the electrochemical sensing system, wherein after the liquid sample is injected into the liquid and the contact electrode is touched, the processor applies a voltage between the working electrodes and is transmitted through the analog digital conversion. The _-current change of the test electrode and the red electrode. The electrochemical sensing system of the item, wherein the measuring device further comprises a processor-display for displaying 11 in the electrode system, an electrochemical sensing system, comprising: One of the electrochemical test strips; the road; the starter device, through the electrochemical test, forms a back-burst device, the ribs are formed between the secret sample and the reference electrode, the identification electrode and the Detecting: an electrical change between the electrode and the detecting electrode; 'Through the operating voltage to supply the voltage to the reference-measuring device' to measure a reference between the reference electrode and the red electrode 4APEX05002TW. Doc 1302604 Current change. 12. A method of using an electrochemical test strip according to claim 1 to sense a liquid sample, comprising the steps of: (a) placing the electrochemical test paper in a measuring device, by the identification electrode, The resistor and the reference electrode form a loop with the measuring device to activate the measuring device; (b) providing the liquid sample in the reaction zone; and (c) applying a voltage to the reference electrode and the working electrode Between, and measure the current corresponding to the voltage. I3. The method of claim 12, wherein the step A (a) and the step (b) further comprise the following steps: (al) measuring the impedance value between the identification electrode and the reference electrode to determine Whether the electrochemical test paper is correctly placed in the measuring device. The method of claim 12, wherein the step (and the step (4) further comprises the step of: (bl) measuring the electrical property between the identification electrode and the reference electrode caused by the addition of the liquid sample The method of claim 12, wherein the method of claim 12, wherein the step (the step (6) further comprises the following steps: 4APEX05002TW.doc 17 1302604. 16. In the method, the step (b) includes the following steps: ^ (b3) measuring an electrical change between the worker, the electrode and the detecting electrode to confirm that the liquid sample covers the reference electrode and the work The method of claim 12, wherein the loop formed by the step (a) turns on a diode, causing a voltage change at one of the positive ends of the diode, and the voltage change initiates the voltage The method of claim 12, wherein the loop formed by the step (8) turns on a transistor to cause a voltage change of one of the collectors of the transistor, and the amount is activated by the voltage change Measuring device. 4APEX05002T W.doc 18