200944591 九、發明說明： 【發明所屬之技術領域】 本發明係有種式生物❹m㈣改良結構，尤指—種能使每 片生物感測試片多次使關改良結構，以增加每片生物感測則的使用次 數，並減少每次檢測費用》 【先前技術】 ❹ 目前所有市面上的血糖檢戦片都被設計成-以能使用-次、用完 試片。這種-片只能使用—次試片對於開發中或未開發國家的糖尿 f者來說，實在太過昂貴、而負擔不起，因而無法定期依據病情作錄 檢測，甚而危及糖尿病患者生命’等到發現病情嚴重時，往往已經來不及 • 了。他們非常需要""種更便宜、可赌常朗的的血糖檢測試片。 我們前-個專辦請案(美國㈣專利中請案號Να1_4，即首次 提出了在單-試片上能多次使用的電化學試片，但内容與本中請案不同。 在上述這個美國專利申請案號Να 10/704,701 +，離電極接聊最遠的第一 個反應區的工作電極或參考電極到電極接腳的電阻比第二個反應區的工作 Ο 電極或參考電極到電極接腳的電阻大。而且，比第一個反應區更接近電極 接腳的第二個反應區的工作電極或參考電極到電極接腳的電阻比最靠近電 極接腳的第三個反應區的工作電極或參考電極到電極接腳的電阻大。這種 在單一試片上能多次使用的電化學試片在實際使用時，在相同的條件下， 會出現第一個反應區的檢測讀值比第二個反應區的檢測讀值低，且第二個 反應區的檢測讀值比第三個反應區的檢測讀值低。因而，我們隨後在中華 民國台灣申請的發明專利（中華民國發明專利申請案號〇9411〇748)中提出 了在單一試片上能多次使用的電化學試片改良設計，讓第一個反應區的工 作電極或參考電極到電極接腳的電阻與第二個反應區的工作電極或參考電 極到電極接腳的電阻相同，且第二個反應區的工作電極或參考電極到電極 接腳的電阻與最靠近電極接腳的第三個反應區的工作電極或參考電極到電 200944591 極接腳的電阻相同。然而，這種在單一試片上能多次使用的電化學試片在 實際使用時，在相同的條件下’仍然會出現第一個反應區的檢測讀值比第 二個反應區的檢測讀值低’且第二個反應區的檢測讀值比第三個反應區的 檢測讀值低。這是因為在使用這種試片時，我們會先測離電極接腳最遠的 第一個反應區。而此時’第二個反應區及第三個反應區上的氧化酶或去氫 酶酵素配方會吸水，造成橫跨第二個反應區及第三個反應區上的工作電極 及參考電極間會有微小的短路。以我們的設計，在以jk液檢測第一個反應 區時’橫跨第一個反應區上的工作電極及參考電極間的電阻約為2萬〜25 萬Ω，而此時’因為吸水造成微小的短路的緣故，橫跨第二個反應區及第 Q 三個反應區上的工作電極及參考電極間的電阻約為2百萬〜6千萬Ω。第一 個反應區上電化學反應產生的電流會與第二個反應區及第三個反應區上二 個微導通而分流，造成在電極接腳處所測得的電流與實際產生的電流小， * 因而檢測讀值較低。再者，在以血液檢測第二個反應區時，由於第一個反 應區已被折斷不復存在’第二個反應區上電化學反應產生的電流會因第三 個反應區上只有一個微導通而分流，造成在電極接腳處所測得鈞電流舆實 際產生的電流小，因而檢測讀值較低，但仍然比第一個反應區讀值高。最 後’在以jk液檢測第三個反應區時’由於第二個反應區又已被折斷不復存 在’第三個反應區上電化學反應產生的電流不會被分流’所以所測到的讀 值最高。結果，在相同的條件下，仍然會出現第一個反應區的檢測讀值比 ® 第二個反應區的檢測讀值低，且第二個反應區的檢測讀值比第三個反應區 的檢測讀值低。我們之前二種專利設計仍有不盡理想之處。Shir〇 Nankai 等人申請的美囪專利5,120,420號揭示了一種在一片試片上有多條電極導 線，以便同時爲數個檢品檢測其血糖濃度，再取其平均值，以增加準確度。 但是這種方法需要比較多的血液量，對於病患在家自我檢測者而言，並不 可行。同時’這種方法也無助於降低使用者的檢測費用。Davies等人申請 的美國專利US2003/0024,811號雖然揭示了一種在一大片試片板上有很多 獨立的獨立小試片’再將每個獨立的小試片分開，此種方式與本專利揭示 的方法不同，也無法達到每個獨立的試片有多次使用的功能eKhan的美國 專利US6,855, 243號揭示了一種試片’其中包含面對面中間隔了一個絕緣 200944591 板的兩個底板’每個底板上分別有一個工作電極及一個參考電極及導線。 但是’這種設計仍然無法於單一式片上作多次測試使用。另外，2^ang等人 的美國專利US6, 670,115號雖然提出了一種三條電極導線以測試兩種不同 檢趙的設計，但是他們的設計並未提出如本發明所述製作不同電阻的導線 設計。除外’ Wohlstadter的美國專利US6,673,533號、Miyashita的美國 專利 US2004/0040866 號、Heller 的美國專利 US5, 972,199 號及 Matzinger 的美國專利US6, 558, 528號也沒有提出如未提出如本發明所述具多次使用 及製作不同電阻的導線設計。 〇 【發明内容】 本發明提出了數種方法能讓使用者於單_電化學生物感測試片上作多 .次測試’其中包含二條或三條導電電極。本創作之上述及其他目的與優點， I從下述圖中’獲得深人了解。其中，相類似的元件會被賦予相同的元件 符號。 【實施方式】 第圖所示第一圖係本發明人於美國發明專利申請案號 :·」704’7G1中所揭露的單一試片能多次使用的生物感測試片，這個試片 反^ ’其上#兩條電極導線2G能料—做舰4G、第二個 化學“μ 反f區6G上的工作電極21及參考電極22上放置的電 學反應訊號傳遞到或去氮酶Dehydr〇ge_)上的電化 接觸，4 這兩個電極接腳30與生物感測儀器相 ==因:r根據所測得的電化學反應訊號轉換成待測物 腳30距離最遠^ 有單位的讀值。第一個反應區4〇與電極接 到電極接腳30的電阻（長度^應區4〇上的工作電極21及參料極22 離較第-個反應區40近，所以最$ ’第二個反應區5〇舆電極接脚30的距 電極22到電極接腳二電所^第;;個妨反應:^的工作電㈣及參考 刃尾阻（長度）較小；第三個反應區60舆電極接腳 8 200944591 30的距離最近’所以’第三個反應區60上的工作電極21及參考電極没到 電極接腳30的電阻（長度）最小。對於相同的電化學反應電壓而言，電阻 越大則電流越小’以電流做判別標準的讀值就越小。因此，從第一個反應 區40測得的讀值會比第二個反應區50小；而從第二個反應區5〇測得的讀 值會比第三個反應區60小。因此，對於檢測相同的待測物質，三個讀值都 不同會導致誤判。所以，本發明人乃提出中華民國發明專利申請案號 094110748的設計，讓第一個反應區40的工作電極21或參考電極22到電 極接腳30的電阻（長度）與第二個反應區50的工作電極21或參考電極22 到電極接腳30的電阻（長度）相同，且第二個反應區50的工作電極21或 〇 參考電極22到電極接腳30的電阻與最靠近電極接腳的第三個反廡區6〇的 工作電極21或參考電極22'到電極接腳3D的電阻（長度)相同，如第二圖 所示。然而’這種在單一試片上能多次使用的電化學試片在實際使用時， . 在相同的條件下，仍然會出現第一個反應區40的檢測讀值比第二個反應區 50的檢測讀值低，且第二個反應區50的檢測讀值比第三個反應區60的檢 測讀值低。這是因為在使用這種試片時’我們會先測離電極接腳3〇最遠的 第一個反應區40。而此時’第二個反應區50及第三個反應區60上的氧化 酶或去氫酶酵素配方會吸水，造成橫跨第二個反應區50及第三個反應區6〇 上的工作電極21及參考電極22間會有微小的短路。以我們的設計，在以 血液檢測第一個反應區40時’橫跨第一個反應區40上的工作電極21及參 ® 考電極22間的電阻約為2萬〜25萬Ω，而此時，因為吸水造成微小的短路 的緣故’橫跨第二個反應區50及第三個反應區60上的工作電極21及參考 電極22間的電阻約為2百萬〜6千萬Ω。第一個反應區40上電化學反應產 生的電流會因第二個反應區50及第三個反應區60上二個微導通而分流， 造成在電極接腳30處所測得的電流與實際產生的電流小，因而檢測讀值較 低。再者，在以血液檢測第二個反應區50時，由於第一個反應區40已被 折斷不復存在’第二個反應區50上電化學反應產生的電流會因第三個反應 區60上只有一個微導通而分流，造成在電極接腳3〇處所測得的電流與實 際產生的電流小’因而檢測讀值較低，但仍然比第一個反應區40讀值高。 最後，在以血液檢測第三個反應區60時，由於第二個反應區50又已被折 200944591 斷不復存在’第三個反應區60上電化學反應產生的電淹不會被分流，所以 所測到的讀值最高。結果，在相同的條件下，仍然會出現第一個反^區4〇 的檢測讀值比第二個反應區50的檢測讀值低，且第二個反應區5〇的檢測 讀值比第三個反應區60的檢測讀值低。我們之前二種專利設計仍有不盡理 想之處。 ❹ ❹ 因此，和rmn不赞明如第三圖的設計，讓第一個反應區4〇上的】 作電極21及參考電極22到與電極接腳30電阻（長度）最小，第二個反肩 區50上的工作電極21及參料極22到電極接腳3〇的電阻（長度）較第 -個反應區4G上的功電極21及參考電極22 _電極接腳3q電阻^ 度）稍大，第三個反應區60上的工作電極21及參考電極⑵到電極接腳3( 2阻（長度m個反應區4G上的X作電極21及參考電極22到 =電極接腳30電阻與第二個反應區5〇上的工作電極21及極尨 電極接腳30的電阻及第三個反應區6〇上的工作電極21及 極拉 電極接腳30的電阻間的差異大小與電極導線2〇的材質有關。電極導線沉 材質的電阻越高，則此電阻間的差異必須較大. 長度來控淋狀舰與電減腳射㈣電阻::導一= =::==極2_電極接腳二= 反廄★參考_ 22到電極接腳30的電阻小，且第二個 反應£ 5G上心作電極21衫考電極& 一 ^ 反應區60上的工作電極21及參考電=接腳30的電阻比第二個 法也可以_導線2_窄來^=與^ __稍小的方 則其電_、㈣極__ 心電糾㈣越寬， 二個反應㈣上的=極 最小，第 -個反應區40上·作^極2122到電極接腳3G的電阻較第 而第三個反 6Q上紅作電極21^22到與電極接襲電阻務大， 最大。第三圖及第四隨_方^考電極22到電極接腳3〇的電阻 2!及參考電極22到與電極接腳3Q電二第2反應·上的工作電極 敢小’從第一個反應區40上的工作 200944591 電極21及參考電極22理應可以產生比第二個反應區50上的工作電極21 及參考電極22傳導出更多的電流’然而因為一部份的電流被第二個反應區 50及第三個反應區60的微導通消耗掉。所以，在電極接腳3〇從第一個反 應區40測得的電流會與電極接腳3〇從第二個反應區50測得的電流相同； 同理’在電極接腳30從第二個反應區50測得的電流會與電及接腳3〇從第 三個反應區60測得的電流相同。這是本發明得以克服上述微導通，而使得 對相同的待測物質而言，這三個反應區才得以測得相同的讀值。這種設計 機制與本發明人之前的美國發明專利申請案號Να10/7〇4, 701及中華民國 發明專利申請案號094110748明顯不同，也從未被揭露過。 〇 第三圖的單一試片能多次使用的設計是將其兩條電極導線20置於同一 片底板10之上。這兩條兩條電極導線20也可以放置在不同的底板丨〇上， 而這兩個底板10面對面，中間以一個絕緣片24隔開，如第五Α圖所示。 第五B圖則是第五A圖中上層蓋板70翻轉180。過來之導電線路的示意圖。 雖然’Khan的美國專利US6,855,243號也揭示了一種類似的試片，但是Khan 的美國專利US6, 855,243號並無法讓單一試片做多次測試使用 。而且，Khan 的美國專利US6, 855,243號也未揭示如第三囷及第四圖在第一、第二及第 三個反應區與電極接腳30間須有不同電阻的設計。 第六圖是第五圖的橫斷面剖面圖，它包含了上下兩個底板10，中間夹 Ο 著一片隔板24,隔板24與底板10間有電極導線20。第六圖的上視圖極下 視囷的左邊各有一電極接腳30，使得第六圖的生物感測試片所產生的電化 學反應訊號需由上下兩個電極接腳30處讀取。待測檢體如血液會從試片第 一個反應區40右端的虹吸口 11藉虹吸引力吸入第一個反應區40與工作電 極21或參考電極22上放置的電化學反應物質25 (如氧化酶Oxidase或去 氩酶Dehydrogenase)起電化學反應’再將電化學反應訊號傳遞到兩個電極 接腳30 ’由測試儀器讀取。工作電極21與參考電極22面對面隔開，且其 面積相當。當第一個反應區40使用過後，第一個反應區40會由第一個反 應區40及第二個反應區50之間的折斷缺角13及折斷孔32將第一個反應 區40折斷，而露出第二個反應區50。第二次測試可由第二個反應區50右 端的虹吸口 11藉虹吸引力吸入第二個反應區40與工作電極21或參考電極 11 200944591 22上放置的電化學反應物質25 (如氧化酶Oxidase或去氫酶 Dehydrogenase)起電化學反應。然後再將第二個反應區50折斷，以測試 第三個反應區60。第六圖的下試圖上有通氣孔31以利待測檢體如血液吸進 第一、第二及第三反應區時的排氣之用。 第五圖是第三圖的另一種將電極導線20放置在面對面的不同底板1〇 上，而第七A圖是第四圖的另一種將電極導線2〇放置在面對面的不同底板 10上的設計。第七B圖是第七A圖中的上層蓋板翻轉180。過來的示意圖， 以看清楚背面的線路結構。 ^ 每片試片能多次使用的電化學式的生物感測試片其電極導線主要分為 二線式和三终式。二線式的生物感測試片的電極導線2〇在每個反應區都有 工作電極21及參考電極22,上述的第一圖到第七圖全是。而三線式的生物 感測試片的電極導線20在每個反應區都有工作電極21、參考電極22及對 應電極23 ’與上述的設計不同。以下介紹幾種每片試片能多次使用、三線 式的電化學式的生物感測試片。 第八A圖，係本發明的試片以電極導線長短控制三個反應區的電阻讓 三個反應區的所測讀值相同的電極導線之設計示意圖，其中每個反應區有 工作電極21、參考電極22及對應電極23，且其中一個電極與電極接腳30 係經由底板背面的電極導線2〇導通。第八圖中三個電極接腳的中間那個電 © 極接腳30有個圓孔36穿孔到底板1〇的背面，如第八B圖所示，與其上的 電極導線20相連通直到另一邊的三個圓孔36上，又分別接到底板10正面 的三個反應區的其中一個電極上。中間的電極接脚3〇能夠透過圓孔36連 到底板10的背面上的電極導線2〇，再透過另一邊的三個圓孔36又分別接 到底板10正面的三個反應區的其中電極上的原因是圓孔36的内壁全塗佈 導電物質’或圓孔36全部填滿導電物質讓底板1〇的兩面的電極及電極導 線20得以導通。第八C圖則是將底板1〇的背面線路以虛線表示以顯示底 板10上的圓孔36如何透過背面的導電線路傳到其他圓孔36上。重要的是， 所有第一個反應區4〇的電極到電極接腳3〇的電阻必須比第二個反應區50 的電極到電極接腳3〇的電阻小，且第二個反應區50的電極到電極接腳30 12 200944591 的電阻必須比第三個反應區60的電極到電極接腳3〇的電阻小。這樣才得 以克服上述微導通之問題，而使得對相同的待測物質而言這三個反應區 才得以測得相同的讀值。 第九Α圖係本發明以電極導線寬窄控制三個反應區的電阻讓三個反應 區的所測讀值相同的試片電極導線之設計示意圖，其中每個反應區有工作 電極21、參考電極22及對應電極23 ’且其中一個電極與電極接脚30係經 由底板背面的電極導線20導通。第九B圖指出了底板10的背面的導電線 路設計；而第九C圖則是將底板1〇的背面線路以虛線表示以顯示底板1〇 上的圓孔36如何透過背面的導電線路傳到其他圓孔36上。第十圖所示的 〇 設計理念幾乎與第八圖相類似，只不過第十圖是以電極導線的寬窄控制讓 二個反應區的電阻不同，而第八圖是以電極導線的長短控制讓三個反應區 的電阻不同》第十圖中三個電極接腳的中間那個電極接腳30有個圓孔36 穿孔到底板10的背面，與其上的電極導線20相連通直到另一邊的三個圓 孔36上，又分別接到底板1〇正面的三個反應區的其中一個電極上。中間 的電極接腳30能夠透過圓孔36連到底板10的背面上的電極導線2〇，再透 過另一邊的三個圓孔36又分別接到底板1〇正面的三個反應區的其中電極 上的原因是圓孔36的内壁全塗佈導電物質，或圓孔36全部填滿導電物質 讓底板10的兩面的電極及電極導線20得以導通》重要的是，所有第一個 反應區4〇的電極到電極接腳30的電阻必須比第二個反應區5〇的電極到電 極接腳30的電阻小，且第二個反應區50的電極到電極接腳3〇的電阻必須 比第三個反應區60的電極到電極接腳30的電阻小。這樣才得以克服上^ 微導通之問題，而使得對相同的待測物質而言，這三個反應區才得以^ 相同的讀值。 ' 第十圖’係本發明以電極導線20的長短控制三個反應區的電阻讓三個 反應區的所測讀值相同的試片電極導線之設計示意圖，其中每個反應&有 工作電極21、參考電極22及對應電極23，且其中一個電極及其電極&線 20與其他兩個電極及其電極導線20係位於相對面、且中間以一絕緣板24 隔開的不同底板10上。仍然必須強調的是，所有第一個反應區4〇的電極 到電極接腳30的電阻必須比第二個反應區50的電極到電極接腳3〇的電阻 13 200944591 小’且第二個反應區5〇的電極到電極接腳30的電阻必須比第三個反應區 60的電極到電極接腳3〇的電阻小。這樣才得以克服上述微導通之問題，而 使得對相同的待測物質而言，這三個反應區才得以測得相同的讀值。 第十一圖，係本發明以電極導線寬窄控制三個反應區的電阻讓三個反 應區的所測讀值相同的試片電極導線之設計示意圖，其中每個反應區有工 作電極21、參考電極22及對應電極23，且其中一個電極及其電極導線與 其他兩個電極及其電極導線係位於相對面、且中間以一絕緣板24隔開的不 同底板10上。第十一圖所示的設計理念幾乎與第九圖相類似，只不過第十 一圖是以電極導線的寬窄控制讓三個反應區的電阻不同，而第九圖是以電 〇 極導線的長短控制讓三個反應區的電阻不同。仍然必須再強調的是，所有 第一個反應區40的電極到電極接腳30的電阻必須比第二個反應區50的電 極到電極接腳30的電阻小，且第二個反應區50的電極到電極接腳30的電 阻必須比第三個反應區60的電極到電極接腳30的電阻小。這樣才得以克 服上述微導通之問題’而使得對相同的待測物質而言，這三個反應區才得 以測得相同的讀值。 由以上詳細說明，可使熟知本項技藝者明瞭本創作的確可達成前述目 的’實已符合專利法之規定，爰提出專利申請。 惟以上所述者，僅為本創作之較佳實施例而已，當不能以此限定本創 ® 作實施之範圍；故，凡依本創作申請專利範園及創作說明書内容所作之簡 單的等效變化與修飾，皆應仍屬本創作專利涵蓋之範圍内β 【圖式簡單說明】 第一圖，係本發明人於美國發明專利申請案號N〇.l〇/7〇4,7〇l中 所揭露的早一試片能多次使用的生物感測試片 第二圖，係本發明人於中華民國發明專利申請案號〇9411〇748中 所揭露的單一試片能多次使用的生物感測試片 第三圖’係本發_電極導線長短控制三個反顧的電阻讓三個反應 區的所測讀值相同的試片電極導線之設計示意圖，其中工作 200944591 電極與參考電極位於同^—底板上β 第四圖’係本發明以電極導線寬窄控制三個反應區的電阻讓三個反應 區的所測讀值相同的試片電極導線之設計示意圖》 第五Α圖’係本發明以電極導線長短控制三個反應區的電阻讓三個反 應區的所測讀值相同的試片電極導線之設計示意圖，其中工 作電極與參考電極位於相對面的不同底板上。 第五B圖，係第五A圖的上蓋板翻轉1別。後背面的線路示意圖， 第六圖’係第五圖組合後的上視、下視、側視及剖面視圖。 第七A囷’係本發明以電極導線寬窄控制三個反應區的電阻讓三個反 應區的所測讀值相同的試片電極導線之設計示意圖，其中工 作電極與參考電極位於相對面的不同底板占。 第七B圖，係第七A圖的上蓋板翻轉180。後背面的線路示意圓。 第八A圖，係本發明以電極導線長短控制三個反應區的電阻讓三個反 應區的所測讀值相同的試片電極導線之設計示意圖，其中每 個反應區有工作、參考及對應電極，且其中一個電極與電極 接腳係經由底板背面的電極導線導通。 第八B圖，係第八A圖的底板翻轉18〇。後背面的線路示意圖。 第八C圖’係第八A圖組合後兩條電極導線及電極的相對位置圓。 第九A圖，係本發明以電極導線寬窄控制三個反應區的電阻讓三個反 應區的所測讀值相同的試片電極導線之設計示意圖，其中每 個反應區有工作、參考及對應電極，且其中一個電極與電極 接腳係經由底板背面的電極導線導通。 第九B圖，係第九A圖的底板翻轉180。後背面的線路示意圖。 第九C圖’係第九A圖組合後兩條電極導線及電極的相對位置圖。 第十A圖，係本發明以電極導線長短控制三個反應區的電阻讓三個反 應區的所測讀值相同的試片電極導線之設計示意圖，其中每 個反應區有工作、參考及對應電極，且其中一個電極及其電 極導線係與其他兩個電極及其電極導線係位於相對面的不 同底板上β 15 200944591 第十B圖，係第十A圖的底板翻轉180°後背面的線路示意圖。 第十C圖，係第十A圖組合後兩條電極導線及電極的相對位置圖。 第十一 A圖，係本發明以電極導線寬窄控制三個反應區的電阻讓三個 反應區的所測讀值相同的試片電極導線之設計示意两，其中 每個反應區有工作、參考及對應電極，且其中一個電極及其 電極導線係與其他兩個電極及其電極導線係位於相對面的 不同底板上。_ 第十一 B圖，係第十一 A圖的底板翻轉18〇。後背面的線路示意圖。 第十一C圖，係第十一 A圖組合後兩條電極導線及電極的相對位置圖。 〇 【主要元件符號說明】 10底板 11虹吸口 13折斷缺角 20電極導線 21工作電極 22參考電極 23對應電極 24絕緣板 〇 25化學反應物質 30電極接脚 31通氣孔 32折斷孔 33缺孔 36圃孔 40第一反應區 50第二反應區 60第三反應區 70上層蓋板 16200944591 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a modified structure of a biological ❹m (4), in particular, a seed that enables each biosensor test piece to be repeatedly modified to increase the structure of each biosensor. The number of uses, and reduce the cost of each test. [Prior Art] ❹ All commercially available blood glucose test strips are designed to be used - to use the test strips. This kind of film can only be used - the test strip is too expensive for the developing or undeveloped countries, and can not afford it, so it can not be diagnosed according to the condition of the disease, even endangering the lives of diabetic patients. When it is found that the condition is serious, it is often too late. They really need "" a cheaper, gambling blood glucose test strip. We have a special case (the US (4) patent request number Να1_4, which is the first time to propose an electrochemical test piece that can be used multiple times on a single-test piece, but the content is different from the case in this case. Patent application No. Να 10/704,701 +, the resistance of the working electrode or reference electrode to the electrode pin of the first reaction zone farthest from the electrode is higher than the working Ο electrode or reference electrode of the second reaction zone to the electrode The resistance of the foot is large. Moreover, the work resistance of the working electrode or the reference electrode to the electrode pin of the second reaction zone closer to the electrode pin than the first reaction zone works than the third reaction zone closest to the electrode pin. The resistance of the electrode or the reference electrode to the electrode pin is large. In the actual use, the electrochemical test piece which can be used multiple times on a single test piece will have the detection ratio of the first reaction zone under the same conditions. The detection reading of the second reaction zone is low, and the detection reading of the second reaction zone is lower than the detection reading of the third reaction zone. Therefore, we subsequently applied for the invention patent in the Republic of China Taiwan (Republic of China invention patent) Application No. 〇9411〇748) proposes an improved design of an electrochemical test strip that can be used multiple times on a single test piece, allowing the resistance of the working electrode or reference electrode to the electrode pin of the first reaction zone to react with the second reaction. The working electrode or reference electrode of the zone has the same resistance to the electrode pin, and the working electrode of the second reaction zone or the resistance of the reference electrode to the electrode pin and the working electrode or reference of the third reaction zone closest to the electrode pin The resistance of the electrode to the electric pole of 200944591 is the same. However, in the actual use of the electrochemical test piece which can be used multiple times on a single test piece, the detection reaction of the first reaction zone will still occur under the same conditions. The value is lower than the detection reading of the second reaction zone and the detection reading of the second reaction zone is lower than the detection reading of the third reaction zone. This is because when using this test piece, we will first measure The first reaction zone farthest from the electrode pin. At this time, the oxidase or dehydrogenase enzyme formula in the second reaction zone and the third reaction zone will absorb water, causing the second reaction zone to be On the third reaction zone There is a slight short circuit between the working electrode and the reference electrode. In our design, when the first reaction zone is detected with jk liquid, the resistance between the working electrode and the reference electrode across the first reaction zone is about 2 10,000 to 250,000 Ω, and at this time, the resistance between the working electrode and the reference electrode across the second reaction zone and the third reaction zone of Q is about 2 million 〜6 because of the slight short circuit caused by water absorption. Ten million ohms. The current generated by the electrochemical reaction in the first reaction zone will be shunted by the two micro-conductings in the second reaction zone and the third reaction zone, resulting in the current measured at the electrode pins and the actual generation. The current is small, so the detection reading is lower. Furthermore, when the second reaction zone is detected by blood, the first reaction zone has been broken and no longer exists. The current will be shunted by the fact that there is only one micro-conduction in the third reaction zone, resulting in a small current measured at the electrode pin, and the actual current generated is small, so the detection reading is lower, but still reading than the first reaction zone. high. Finally, 'when the third reaction zone is detected with jk liquid', the second reaction zone has been broken and no longer exists. 'The current generated by the electrochemical reaction in the third reaction zone is not shunted', so the measured The reading is the highest. As a result, under the same conditions, the detection reading of the first reaction zone will still be lower than the detection reading of the second reaction zone, and the detection reading of the second reaction zone is lower than that of the third reaction zone. The detection reading is low. Our previous two patent designs still have some unsatisfactory results. Shir〇 Nankai et al., U.S. Patent No. 5,120,420, discloses a plurality of electrode wires on a test piece for simultaneously measuring the blood glucose concentration of several samples and then taking the average value thereof to increase the accuracy. However, this method requires a relatively large amount of blood, which is not feasible for patients who are self-testing at home. At the same time, this method does not help to reduce the user's testing costs. U.S. Patent No. 2003/0024,811, issued to Davies et al., discloses the disclosure of the entire entire entire entire entire entire entire entire entire entire entire entire entire entire entire entire The method disclosed is also different, and it is not possible to achieve the function of using multiple independent test strips. The US Patent No. 6,855,243 discloses a test piece which comprises two bottom plates spaced apart in a face-to-face with an insulating 200944591 plate. 'Each bottom plate has a working electrode and a reference electrode and wire. However, this design still cannot be used for multiple tests on a single chip. In addition, U.S. Patent No. 6,670,115, the disclosure of which is incorporated herein by reference in its entirety in its entirety, the utility of the utility of the utility of the utility of the utility of the utility of the utility of the present invention. Except for U.S. Patent No. 6,673,533 to Wohlstadter, U.S. Patent No. 2004/0040, 866, to Miyashita, U.S. Patent No. 5,972, 199 to Heller, and U.S. Patent No. 6,558,528 to Matzinger, et al. Wire design with multiple uses and different resistances. SUMMARY OF THE INVENTION The present invention proposes several methods for allowing a user to perform multiple tests on a single-electrochemical biosensor test sheet, which includes two or three conductive electrodes. The above and other objects and advantages of the present invention are obtained from the following figures. Among them, similar components will be given the same component symbol. [Embodiment] The first figure shown in the figure is a biosensor test piece which can be used for a plurality of times in a single test piece disclosed in the U.S. Patent Application Serial No.: "704'7G1". 'The upper two wires 2G energy material - the ship 4G, the second chemical "μ anti-f zone 6G on the working electrode 21 and the reference electrode 22 placed on the electrical reaction signal to or deaminase Dehydr〇ge_ Electrochemical contact on the 4, the two electrode pins 30 and the bio-sensing instrument phase == because: r according to the measured electrochemical reaction signal converted to the farthest distance of the object to be tested 30 distance ^ has a unit of reading The resistance of the first reaction zone 4〇 and the electrode to the electrode pin 30 (the length of the working electrode 21 and the reference electrode 22 on the length 4应 are closer to the first reaction zone 40, so the most The two reaction zones 5 〇舆 electrode pin 30 are from the electrode 22 to the electrode pin 2; the reaction is: ^ the working power (4) and the reference blade tail resistance (length) is small; the third reaction Zone 60舆 electrode pin 8 200944591 30 distance closest 'so' the third electrode 60 of the working electrode 21 and the reference electrode did not arrive The resistance (length) of the electrode pin 30 is the smallest. For the same electrochemical reaction voltage, the larger the resistance, the smaller the current. The smaller the reading value is, the smaller the reading value is. Therefore, from the first reaction zone 40 The measured reading will be smaller than the second reaction zone 50; and the reading from the second reaction zone 5〇 will be smaller than the third reaction zone 60. Therefore, for detecting the same substance to be tested, three The fact that the readings are different can lead to misjudgment. Therefore, the inventors have proposed the design of the Republic of China invention patent application No. 094110748 to allow the resistance of the working electrode 21 or the reference electrode 22 of the first reaction zone 40 to the electrode pin 30 ( The length) is the same as the resistance (length) of the working electrode 21 or the reference electrode 22 to the electrode pin 30 of the second reaction zone 50, and the working electrode 21 or the 〇 reference electrode 22 to the electrode pin 30 of the second reaction zone 50 The resistance is the same as the resistance (length) of the working electrode 21 or the reference electrode 22' to the electrode pin 3D of the third 庑 region 6 最 closest to the electrode pin, as shown in the second figure. Electrochemical test that can be used multiple times on a single test piece In actual use, under the same conditions, the detection reading of the first reaction zone 40 will still be lower than the detection reading of the second reaction zone 50, and the detection reading ratio of the second reaction zone 50 The detection reading of the third reaction zone 60 is low. This is because when using this test piece, we will first measure the first reaction zone 40 farthest from the electrode pin 3〇. At this time, the second The oxidase or dehydrogenase enzyme formulation on the reaction zone 50 and the third reaction zone 60 will absorb water, resulting in a gap between the working electrode 21 and the reference electrode 22 across the second reaction zone 50 and the third reaction zone 6〇. There will be a slight short circuit. In our design, when the first reaction zone 40 is detected by blood, the resistance between the working electrode 21 and the reference electrode 22 across the first reaction zone 40 is about 20,000~ 250,000 Ω, at this time, because of the slight short circuit caused by water absorption, the resistance between the working electrode 21 and the reference electrode 22 across the second reaction zone 50 and the third reaction zone 60 is about 2 million~ 60 million Ω. The current generated by the electrochemical reaction on the first reaction zone 40 is shunted by the two microconductions in the second reaction zone 50 and the third reaction zone 60, resulting in the current measured at the electrode pin 30 and the actual generation. The current is small and the detection reading is low. Furthermore, when the second reaction zone 50 is detected by blood, the first reaction zone 40 has been broken and no longer exists. The current generated by the electrochemical reaction on the second reaction zone 50 is due to the third reaction zone 60. Only one micro-conducting is shunted, causing the current measured at the electrode pin 3〇 to be smaller than the actual generated current' thus detecting a lower reading value, but still reading higher than the first reaction zone 40. Finally, when the third reaction zone 60 is detected by blood, since the second reaction zone 50 has been broken by 200944591, the electric flooding generated by the electrochemical reaction in the third reaction zone 60 will not be shunted. Therefore, the measured value is the highest. As a result, under the same conditions, the detection reading of the first inversion region 4〇 is lower than the detection reading value of the second reaction region 50, and the detection reading value of the second reaction region 5〇 is lower than that of the second reaction region. The detection readings of the three reaction zones 60 are low. Our previous two patent designs still have some unreasonable ideas. ❹ ❹ Therefore, and rmn do not like the design of the third figure, so that the resistance (length) of the electrode 21 and the reference electrode 22 to the electrode pin 30 in the first reaction zone 4 is the smallest, the second The resistance (length) of the working electrode 21 and the reference electrode 22 to the electrode pin 3〇 on the shoulder region 50 is slightly lower than the resistance electrode (the working electrode 21 and the reference electrode 22_electrode pin 3q on the first reaction zone 4G). Large, working electrode 21 and reference electrode (2) on the third reaction zone 60 to the electrode pin 3 (2 resistance (the length of the X reaction electrode 4G X electrode 21 and the reference electrode 22 to = electrode pin 30 resistance and The difference between the resistance of the working electrode 21 and the pole electrode pin 30 on the second reaction zone 5〇 and the resistance between the working electrode 21 and the pole pull electrode pin 30 on the third reaction zone 6〇 and the electrode lead Related to the material of 2〇. The higher the resistance of the electrode wire sinking material, the greater the difference between the resistors. The length to control the shower ship and the electric foot reduction (4) Resistance:: lead one ==::== pole 2 _Electrode pin 2 = 廄 廄 ★ reference _ 22 to the electrode pin 30 has a small resistance, and the second reaction £ 5G on the core electrode 21 shirt test electrode & The resistance of the working electrode 21 and the reference electric=pin 30 on the reaction zone 60 may be lower than that of the second method _ wire 2_ narrower ^= and ^ __ is slightly smaller, then the electric _, (four) pole __ The wider the electrocardiogram (4), the smallest on the two reactions (4), the least on the first reaction zone 40, the resistance of the electrode 2122 to the electrode pin 3G, and the third anti-6Q red electrode 21^22 to the electrode with the resistance is large, the largest. The third and fourth with the _ square ^ electrode 22 to the electrode pin 3 〇 resistance 2! and the reference electrode 22 to the electrode pin 3Q electric two 2Reaction·Working electrode is dare to be small 'Working from the first reaction zone 40 200944591 The electrode 21 and the reference electrode 22 are supposed to generate more conduction than the working electrode 21 and the reference electrode 22 on the second reaction zone 50. The current 'but because a portion of the current is consumed by the microconduction of the second reaction zone 50 and the third reaction zone 60. Therefore, the current measured at the electrode pin 3〇 from the first reaction zone 40 will The current measured from the second reaction zone 50 is the same as that of the electrode pin 3〇; similarly, the current measured at the electrode pin 30 from the second reaction zone 50 will be the same as the current. The current measured by the third reaction zone 60 is the same. This is the invention that overcomes the above micro-conduction so that the same reaction can be detected for the same reaction substance. This design mechanism is significantly different from the inventor's prior US invention patent application number Να10/7〇4, 701 and the Republic of China invention patent application number 094110748, and has never been disclosed. The design of the test piece that can be used multiple times is to place the two electrode wires 20 on the same substrate 10. The two electrode wires 20 can also be placed on different bottom plates, and the two substrates 10 Face to face, the middle is separated by an insulating sheet 24, as shown in the fifth figure. The fifth B plan is the upper cover 70 of the fifth A shown in FIG. A schematic diagram of the conductive line coming over. A similar test piece is also disclosed in U.S. Patent No. 6,855,243, the disclosure of which is incorporated herein by reference to U.S. Patent No. 6,855,243 to Khan. Also, U.S. Patent No. 6,855,243 to Khan does not disclose the design of the first and second and third reaction zones and electrode pins 30 having different electrical resistances as in the third and fourth figures. The sixth drawing is a cross-sectional view of the fifth drawing, which includes the upper and lower bottom plates 10 with a partition 24 interposed therebetween, and an electrode lead 20 between the partition plate 24 and the bottom plate 10. The upper view of the sixth figure has an electrode pin 30 on the left side of the view, so that the electrochemical reaction signal generated by the biosensor test piece of the sixth figure needs to be read by the upper and lower electrode pins 30. The sample to be tested, such as blood, is drawn into the first reaction zone 40 from the siphon port 11 at the right end of the first reaction zone 40 of the test strip, and the electrochemical reaction substance 25 placed on the working electrode 21 or the reference electrode 22 (eg, oxidized). The enzyme Oxidase or Dehydrogenase acts as an electrochemical reaction 'and then transmits the electrochemical reaction signal to the two electrode pins 30' to be read by the test instrument. The working electrode 21 is spaced face to face from the reference electrode 22 and has an equivalent area. When the first reaction zone 40 is used, the first reaction zone 40 will break the first reaction zone 40 from the broken corners 13 and the fracture holes 32 between the first reaction zone 40 and the second reaction zone 50. And exposing the second reaction zone 50. The second test can be taken from the siphon port 11 at the right end of the second reaction zone 50 by the x-portion attraction into the second reaction zone 40 and the electrochemical reaction substance 25 placed on the working electrode 21 or the reference electrode 11 200944591 22 (such as oxidase Oxidase or The dehydrogenase dehydrogenase acts as an electrochemical reaction. The second reaction zone 50 is then broken to test the third reaction zone 60. The sixth figure attempts to have a venting opening 31 for the purpose of exhausting the sample to be tested, such as blood, into the first, second and third reaction zones. The fifth figure is another example of the third figure in which the electrode wires 20 are placed on different face plates 1 facing each other, and the seventh A is the other of the fourth figure, the electrode wires 2 are placed on different face plates 10 facing each other. design. Figure 7B is the upper cover flip 180 of Figure 7A. Come over the schematic to see the line structure on the back. ^ Electrochemical biosensor test strips that can be used multiple times per test piece are mainly divided into two-wire and three-final. The electrode lead 2 of the two-line biosensor test piece has a working electrode 21 and a reference electrode 22 in each reaction zone, and the above first to seventh figures are all. The electrode lead 20 of the three-wire biosensor test strip has a working electrode 21, a reference electrode 22, and a counter electrode 23' in each reaction zone different from the above design. The following describes several electrochemical-type biosensor test strips that can be used multiple times for each test piece and three-wire type. Figure 8A is a schematic view showing the design of the electrode wires of the test strips of the present invention in which the resistance of the three reaction zones is controlled by the length of the electrode wires so that the measured readings of the three reaction zones are the same, wherein each reaction zone has a working electrode 21, The reference electrode 22 and the counter electrode 23, and one of the electrodes and the electrode pin 30 are electrically connected via the electrode lead 2 of the back surface of the bottom plate. In the eighth figure, the middle electrode of the three electrode pins has a circular hole 36 which is perforated to the back surface of the bottom plate 1 , as shown in FIG. 8B, and communicates with the electrode wire 20 thereon until the other side The three circular holes 36 are respectively connected to one of the three reaction zones on the front side of the bottom plate 10. The middle electrode pin 3〇 can be connected to the electrode lead 2〇 on the back surface of the bottom plate 10 through the circular hole 36, and then connected to the middle electrode of the three reaction areas on the front surface of the bottom plate 10 through the three round holes 36 on the other side. The reason for this is that the inner wall of the circular hole 36 is entirely coated with the conductive material' or the round hole 36 is completely filled with the conductive material to allow the electrodes on both sides of the bottom plate 1 and the electrode lead 20 to be turned on. The eighth C diagram shows the back line of the bottom plate 1 by dashed lines to show how the circular holes 36 in the bottom plate 10 are transmitted to the other circular holes 36 through the conductive lines on the back side. It is important that the resistance of the electrode to the electrode pin 3 of all the first reaction zones 4 必须 must be smaller than the resistance of the electrode to the electrode pin 3 of the second reaction zone 50, and the second reaction zone 50 The resistance of the electrode to electrode pin 30 12 200944591 must be smaller than the resistance of the electrode to electrode pin 3 of the third reaction zone 60. This is to overcome the above problem of micro-conduction, so that the same reading can be measured for the three reaction zones of the same substance to be tested. The ninth diagram is a schematic diagram of the design of the electrode lead of the test piece in which the resistance of the three reaction zones is controlled by the width of the electrode lead to make the measured reading values of the three reaction zones the same, wherein each reaction zone has a working electrode 21 and a reference electrode. 22 and the corresponding electrode 23' and one of the electrodes and the electrode pin 30 are electrically connected via the electrode lead 20 on the back surface of the bottom plate. The ninth B diagram indicates the conductive line design of the back side of the bottom plate 10; and the ninth C picture shows the back line of the bottom plate 1〇 as a broken line to show how the circular hole 36 on the bottom plate 1 is transmitted through the conductive line on the back side. Other round holes 36. The design concept of 〇 shown in the tenth figure is almost similar to that of the eighth figure, except that the tenth figure shows that the resistance of the two reaction zones is different by the width and narrowness of the electrode wires, and the eighth figure is based on the length of the electrode wires. The resistance of the three reaction zones is different. In the middle of the three electrode pins in the tenth figure, the electrode pin 30 has a circular hole 36 which is perforated to the back surface of the bottom plate 10, and communicates with the electrode wire 20 thereon until three on the other side. The circular holes 36 are respectively connected to one of the three reaction zones on the front side of the bottom plate 1 . The middle electrode pin 30 can be connected to the electrode lead 2〇 on the back surface of the bottom plate 10 through the circular hole 36, and then connected to the middle electrode of the three reaction areas on the front side of the bottom plate 1 through the three round holes 36 on the other side. The reason for this is that the inner wall of the circular hole 36 is entirely coated with a conductive substance, or the round hole 36 is completely filled with the conductive material to allow the electrodes on both sides of the bottom plate 10 and the electrode lead 20 to be turned on. Importantly, all the first reaction zones are 〇 The resistance of the electrode to the electrode pin 30 must be smaller than the resistance of the electrode to the electrode pin 30 of the second reaction zone 5, and the resistance of the electrode of the second reaction zone 50 to the electrode pin 3 must be higher than that of the third The resistance of the electrode to the electrode pin 30 of the reaction zone 60 is small. In this way, the problem of the micro-conduction is overcome, so that the three reaction zones can be read the same for the same substance to be tested. 'Tenth diagram' is a schematic diagram of the design of the electrode lead of the test piece in which the resistance of the three reaction zones is controlled by the length of the electrode lead 20 so that the measured reading values of the three reaction zones are the same, wherein each reaction & has a working electrode 21. Reference electrode 22 and corresponding electrode 23, and one of the electrodes and its electrode & line 20 and the other two electrodes and their electrode leads 20 are located on opposite sides of the substrate 10 separated by an insulating plate 24 . It must still be emphasized that the resistance of the electrode-to-electrode pin 30 of all the first reaction zones must be smaller than the resistance of the electrode-to-electrode pin 3 of the second reaction zone 50, 200914591' and the second reaction The resistance of the electrode 5 to the electrode pin 30 must be smaller than the resistance of the electrode to the electrode pin 3 of the third reaction zone 60. In this way, the above micro-conducting problem can be overcome, so that the same reading value can be measured for the same reaction substance. Eleventh drawing is a schematic diagram of the design of the electrode lead of the test piece in which the resistance of the three reaction zones is controlled by the width of the electrode lead so that the measured reading values of the three reaction zones are the same, wherein each reaction zone has a working electrode 21, reference The electrode 22 and the corresponding electrode 23, and one of the electrodes and the electrode lead thereof are located on opposite sides of the other two electrodes and their electrode leads, and are separated by an insulating plate 24. The design concept shown in Figure 11 is almost similar to the ninth figure, except that the eleventh figure is based on the width and narrowness of the electrode wires to make the resistance of the three reaction zones different, and the ninth figure is the electric dipole wire. The length control makes the resistance of the three reaction zones different. It must still be emphasized that the resistance of the electrode-to-electrode pins 30 of all of the first reaction zones 40 must be less than the resistance of the electrode-to-electrode pins 30 of the second reaction zone 50, and the second reaction zone 50 The resistance of the electrode to the electrode pin 30 must be smaller than the resistance of the electrode to the electrode pin 30 of the third reaction zone 60. In this way, the above problem of microconduction can be overcome, so that the same reaction reading can be measured for the same substance to be tested. From the above detailed description, it will be apparent to those skilled in the art that the present invention can achieve the above-mentioned objectives and that the patent application has been filed. However, the above is only the preferred embodiment of the present invention, and it is not possible to limit the scope of the implementation of this product; therefore, the simple equivalent of the patent application garden and the content of the creation manual according to this creation. Changes and modifications shall remain within the scope of this patent. β [Simple Description] The first figure is the inventor's invention patent application number N〇.l〇/7〇4,7〇l The second picture of the biosensor test piece which can be used repeatedly in the early test piece disclosed in the above is a biological article which can be used multiple times by the single test piece disclosed in the inventor's invention patent application No. 9411〇748. The third test chart of the test piece is based on the design of the electrode wire of the test piece with the same measured reading value of the three reaction zones. The working electrode 200944591 is located at the same time as the reference electrode. ^—β on the bottom plateFig. 4 is a schematic diagram of the design of the electrode wire of the test piece with the same measured reading value of the three reaction zones by the width of the electrode wire to control the width of the electrode wire. Electrode guide Three control resistor length of the reaction zone the reaction zone so that the three readings measured in the same test strip design schematic of the electrode lead, wherein the working electrode and the reference electrode are located on a different circuit board opposite faces. Figure 5B shows the top cover of Figure 5 flipped over. The schematic diagram of the circuit on the back side, the sixth figure is the top view, bottom view, side view and cross-sectional view of the combination of the fifth figure. The seventh embodiment is a schematic diagram of the design of the electrode lead of the test piece in which the resistance of the three reaction zones is controlled by the width of the electrode lead to make the measured reading values of the three reaction zones the same, wherein the working electrode and the reference electrode are located on opposite sides. The bottom plate occupies. Figure 7B is an upper cover flip 180 of Figure 7A. The lines on the back side are rounded. Figure 8A is a schematic view showing the design of the electrode lead of the test piece in which the resistance of the three reaction zones is controlled by the length of the electrode wire so that the measured reading values of the three reaction zones are the same, wherein each reaction zone has a work, reference and corresponding The electrodes, and one of the electrodes and the electrode pins are electrically connected via the electrode wires on the back side of the bottom plate. In the eighth picture B, the bottom plate of Figure 8A is flipped 18 turns. Schematic diagram of the circuit on the back. The eighth C diagram is the relative positional circle of the two electrode wires and the electrodes after the combination of the eighth A picture. Figure IX is a schematic diagram of the design of the electrode lead of the test piece in which the resistance of the three reaction zones is controlled by the width of the electrode lead to make the measured reading values of the three reaction zones the same, wherein each reaction zone has a work, reference and corresponding The electrodes, and one of the electrodes and the electrode pins are electrically connected via the electrode wires on the back side of the bottom plate. Figure IX is a bottom plate flip 180 of Figure IX. Schematic diagram of the circuit on the back. The ninth C diagram is a relative position diagram of the two electrode wires and the electrodes after the combination of the ninth A picture. Figure 10 is a schematic view showing the design of the electrode wires of the test piece in which the resistance of the three reaction zones is controlled by the length of the electrode wire so that the measured reading values of the three reaction zones are the same, wherein each reaction zone has a work, reference and corresponding Electrode, and one of the electrodes and its electrode lead is located on the opposite bottom plate of the other two electrodes and their electrode leads. β 15 200944591 Figure XB, the bottom plate of the tenth A is flipped by 180° schematic diagram. The tenth C diagram is a relative position diagram of the two electrode wires and the electrodes after the combination of the tenth A picture. In the eleventh A, in the present invention, the resistance of the three reaction zones is controlled by the width of the electrode wires, and the design of the electrode wires of the test pieces having the same measured reading values in the three reaction zones is schematically illustrated, wherein each reaction zone has a work and reference. And the corresponding electrode, and one of the electrodes and the electrode lead thereof and the other two electrodes and the electrode lead thereof are located on different bottom plates on opposite sides. _ The eleventh B picture, the bottom plate of the eleventh A picture is flipped 18 〇. Schematic diagram of the circuit on the back. Figure 11C shows the relative position of the two electrode wires and electrodes after the combination of the eleventh A picture. 〇 [Main component symbol description] 10 bottom plate 11 siphon opening 13 broken corner 20 electrode wire 21 working electrode 22 reference electrode 23 corresponding electrode 24 insulating plate 〇 25 chemical reaction material 30 electrode pin 31 vent hole 32 broken hole 33 missing hole 36 Pupil 40 first reaction zone 50 second reaction zone 60 third reaction zone 70 upper cover plate 16