TWI385385B - Bio-sensing device capable of detecting sensing code and short fill - Google Patents

Description

Biosensor device capable of detecting test chip code and insufficient sample filling

The invention relates to a biological sensing test piece and a display processing device thereof, in particular to a detectable test piece code and a sample filling insufficient creature capable of automatically detecting a test piece code and detecting whether the sample filling amount is sufficient. Sensing device.

Due to the rapid development of sensor technology in recent years, various biosensors utilizing enzyme redox reactions have been continuously proposed and successively introduced, among which biosensors in the biomedical field are the most diverse, for example : Blood glucose sensor, which can be used to check the blood sugar level in the blood. Therefore, the blood glucose sensor becomes an important device for self-testing of diabetic patients, which can help diabetic patients self-test the concentration of blood sugar in the body, so that diabetic patients can Always pay attention to and control the blood sugar concentration in the body to maintain the blood sugar concentration within the normal range. Once the patient finds that the blood sugar level of the body is too high, the blood sugar level can be controlled by diet, exercise and insulin injection. However, these controls must be approved by the doctor's supervision and advice.

Please refer to the first and second figures, which are a perspective view of a conventional blood glucose sensing device and an exploded view of a conventional blood glucose sensing test piece. A conventional blood glucose sensing device 1' includes: a blood glucose sensing test piece. 12' and a processing display unit 10', wherein the blood glucose sensing test piece 12' comprises: a strip substrate 122', a reactive layer 124', a spacer 126', and a cover 128'. An electrode portion 1221' is disposed at a front end of the strip substrate 122'. The electrode portion 1221' is sequentially covered with the reaction layer 124', the spacer 126' and the cover plate 128', and the electrode portion An operating electrode 1222' and a corresponding electrode 1224' are disposed on the upper surface of the strip 1212'. The operating electrode 1222' and the corresponding electrode 1224' are respectively connected to a first wire 1226' and a second wire at the tail end of the strip substrate 122'. 1228'. Further, the reaction layer 124' overlying the electrode portion 1221' has an enzyme enzyme to carry out an enzyme redox reaction.

When the diabetic patient uses the above-described blood glucose sensing device 1', the blood glucose sensing test piece 12' is first inserted into the processing display unit 10', and then the patient must first test the blood glucose test piece 12' A strip code is input to the processing display unit 10', and then the blood of the body is taken, and the blood is directly dropped on the reaction layer 124' of the blood glucose sensing test piece 12', and is electrified over a period of time. After the reaction, the processing display unit 10' can calculate the blood glucose concentration in the blood by reading the current change generated by the electrochemical reaction.

The conventional blood glucose sensing device described above is a relatively practical and accurate blood glucose sensing device, however, its main disadvantages are:

1. The test piece code of the blood glucose test piece cannot be automatically detected. When the diabetic patient uses the conventional blood glucose sensing device, the test piece code of the blood glucose test piece must be input before the blood drops into the reaction layer. In order to correct the blood glucose concentration, however, once the patient enters the test piece code error, it will inevitably affect the accuracy of the blood glucose concentration in the blood calculated by the processing display unit. In severe cases, the patient will be mistakenly judged for the condition of diabetes. And there is a regrettable situation.

2. There is no device or mechanism for automatically detecting whether the blood completely covers the reaction layer. If the blood does not completely cover the reaction layer, it indicates that the blood injection amount is insufficient, which is bound to affect the accuracy of the blood glucose concentration calculation by the treatment display unit.

Therefore, the inventors of the present invention have invented the invention in view of the above-mentioned conventional blood glucose sensing device, and finally researched and developed the invention, and finally developed a biosensor for detecting the testable chip code and the insufficient filling of the sample of the present invention. Device.

The main object of the present invention is to provide a bio-sensing device capable of detecting a test chip and a lack of filling of a sample, and adding a sensing code resistor and a third identification film to a biosensor test piece, so that A processing display unit can automatically read one of the biosensor test pieces and automatically detect whether the injection amount of the sample on the biosensor test piece is sufficient, so that the processing display unit can accurately calculate the sample The amount of value.

Another object of the present invention is to provide a bio-sensing device capable of detecting a test chip and a lack of filling of a sample, which is provided with a third identification film on a biosensor test piece and processed in a display unit. Adding a plurality of sensing code resistors, so that the processing display unit can automatically read one of the biosensor test pieces and automatically detect whether the injection amount of the sample on the biosensor test piece is sufficient, so as to process the display. The unit is able to accurately calculate the magnitude of the specimen.

In order to achieve the main object of the present invention, the inventor of the present invention proposes a biosensor device capable of detecting a test chip and a sample filling shortage, comprising: a biosensor test piece and a processing display unit, wherein the biological sense The test piece includes: a first electrode having a first identification film for electrochemically reacting with a sample; and a second electrode having a second identification film for electrochemically reacting with the sample; Measuring resistance, the resistance value of the sensing code resistance can represent one of the biosensor test pieces; a third electrode having a third identification film for electrochemical reaction with the sample; The body flow channel surrounds the first identification film, the second identification film and the third identification film, and when the sample is injected on the biosensor test piece, it can sequentially cover the first flow path along the sample flow path. The identification film, the second identification film and the third identification film; and a siphon air hole, wherein the siphon air hole can utilize a siphon phenomenon to accelerate the sample in the sample flow path, sequentially covering the first identification film and the second identification Membrane and third identification film; the processing display unit package a first reading circuit electrically connected to the sensing code resistor through the third electrode to obtain a resistance value of the sensing code resistance, and when a sufficient amount of the sample 2 is injected into the biosensor test piece, The first reading circuit can detect the electrochemical reaction on the third electrode and output a first voltage signal, and a second reading circuit, when the sample flow path is covered to the third identification film. The second reading circuit can read the electrochemical reaction on the first electrode and output a second voltage signal after the sample is injected into the biosensor test piece.

In order to achieve another object of the present invention, the inventor of the present invention proposes a biosensing device capable of detecting a test chip and a lack of filling of a sample, comprising: a biosensor test piece and a process display unit, wherein The biosensor test piece comprises: a first electrode having a first identification film for electrochemically reacting with a sample; and a second electrode having a second identification film for electrochemically reacting with the sample; a third electrode having a third identification film for electrochemically reacting with the sample; a sample flow path surrounding the first identification film, the second identification film, and the third identification film When the sample is injected onto the biosensor test piece, the first identification film, the second identification film and the third identification film may be sequentially covered along the sample flow path; a siphon hole, the siphon hole may utilize a siphon phenomenon , in order to accelerate the sample in the sample flow channel, sequentially covering the first identification film, the second identification film and the third identification film; and a finger electrode, which is composed of a plurality of single electrodes, wherein Between the adjacent ones of the electrodes, a cutting path is provided The cutting track can separate the adjacent single electrode and break the circuit; the processing display unit comprises: a first reading circuit, when a sufficient amount of the sample is injected into the biosensor test piece, and follows the sample flow path When the third identification film is covered, the first reading circuit can detect the electrochemical reaction on the third electrode and output a first voltage signal; a second reading circuit can be electrically connected to the a first electrode, after the sample is injected into the biosensor test piece, the second read circuit can read the electrochemical reaction on the first electrode, thereby outputting a second voltage signal; and a plurality of sensing code resistors, the plurality The sensing code resistors are connected in series with each other and coupled to the first reading circuit, and the combination of the resistance values of the sensing code resistors may represent one of the sensing chips of the biosensor test piece.

In order to more clearly describe a biometric sensing device of the present invention which is capable of detecting test chip codes and insufficient sample filling, a preferred embodiment of the present invention will be described in detail below with reference to the drawings.

The present invention has two embodiments. Please refer to the third figure, which is a structural diagram of a first embodiment of a biosensor sensing device capable of detecting a test chip and a sample filling insufficiency, and a detectable test piece. The biosensor device 1 with insufficient code and sample filling includes a biosensor test strip 11 and a processing display unit 12.

Please refer to the third figure, and refer to the fourth figure, which is a top view of a first embodiment of a biosensor test piece. In the first embodiment of the present invention, the biosensor test piece 11 includes: a first An electrode 111, a second electrode 112, a sensing code resistor Rs, a third electrode 113, a sample flow path 114, and a siphon air hole 115, wherein the first electrode 111 can be electrically connected to the processing display unit An internal electronic circuit 12, the first electrode 111 has a first recognition membrane, and the 1111 can react with a sample 2, in the embodiment, the sample 2 is blood; the second electrode 112 The second electrode 112 has a second recognition film 1112 for reacting with the sample 2; the sensing code resistor Rs is connected to the second electrode 112 at one end thereof. The resistance value of the code resistance Rs may represent a test piece code of the biosensor test piece 11. wherein the sense code resistor Rs may be replaced by a sense code capacitor or a sense code inductor, and the capacitance value and the inductance value are The same can be used to indicate the test piece code; the third electrode 113 is connected The other end of the sensing code resistor Rs, the third electrode 113 has a third recognition film 1113 that can react with the sample 2, and the third electrode 113 can be connected to the electronic circuit to identify the third The result of the electrochemical reaction between the film 1113 and the sample 2 is transmitted to the electronic circuit. In the embodiment, the first identification film 1111 and the second identification film 1112 are used to verify the main identification film of the sample 2, and the third The identification film 1113 is used to detect whether the sample 2 has completely covered the first identification film 1111 and the second identification film 1112; the first electrode 111 is a working electrode, and the second electrode 112 corresponds to One of the working electrodes is a counter electrode, and the third electrode 113 serves as a sensing electrode to assist the processing display unit 12 to automatically obtain the test piece code, and assists in processing the display unit 12 to detect the third identification film. Whether 1113 has reacted with the sample 2 to determine whether the sample 2 completely covers the first identification film 1111 and the second identification film 1112.

Continuing to refer to the third and fourth figures, the sample flow path 114 is disposed on the first electrode 111, the second electrode 112 and the third electrode 113, and the sample flow path 114 surrounds the first An identification film 1111, the second identification film 1112 and the third identification film 1113, when a sufficient amount of the sample 2 is injected onto the biological sensing test piece 11, it can be sequentially covered along the sample flow path 114. The first identification film 1111, the second identification film 1112 and the third identification film 1113 are electrochemically reacted therewith; the siphon air holes 115 are disposed above the first electrode 111, the second electrode 112 and the third electrode 113, and Close to the third identification film 1113, the siphon air hole 114 can accelerate the sample 2 in the sample flow path 114 by the siphon phenomenon, and sequentially cover the first identification film 1111 to the third identification film 1113.

Please refer to the third and fourth figures, the processing display unit 12 can be inserted into the biosensor test piece 11 to process the electrochemical reaction between the sample 2 and the identification film, in the first embodiment of the present invention. The processing display unit 12 includes a first reading circuit 121, a second reading circuit 122, a micro processing unit 123, and a display 124. The first reading circuit 121 can be electrically connected. The first reading circuit 121 is connected to the sensing code resistor Rs through the third electrode 113 to obtain the sensing code resistance Rs. a resistance value; and when a sufficient amount of the sample 2 is injected into the biosensor test piece 11 and follows the sample flow path 114 sequentially covering the first identification film 1111 to the third identification film 1113, the first reading The circuit 121 can detect the electrochemical reaction on the third electrode 113 and output a first voltage signal.

Continuing to refer to the third and fourth figures at the same time, the second reading circuit 122 of the processing display unit 12 can be electrically connected to the first electrode 111 when the sample 2 has not been injected into the biosensor test piece 11 The second reading circuit 122 cannot read any electrochemical reaction signal, and after the sample 2 is injected into the biological sensing test piece 11, the second reading circuit 122 can read the electrochemical reaction on the first electrode 111. And outputting a second voltage signal; the micro processing unit 123 is coupled to the first reading circuit 121 and the second reading circuit 122. When the sample 2 has not dropped into the biosensor test piece 11, the microprocessor The resistance value of the sensing code resistor Rs is obtained by the third electrode 113, and the test piece code of the biosensor test piece 11 is obtained after the calculation, and the microprocessor 123 can receive the first voltage signal and the The second voltage signal, when the microprocessor 123 receives the second voltage signal, indicates that the sample 2 is electrochemically reacted with the first identification film 1111 or the second identification film 1112, and then, when the microprocessor 123 receives the first voltage signal again, that is, the sample 2 has covered the The first identification film 1113 is electrochemically reacted therewith, which also means that the sample 2 injected into the biosensor test piece 11 is sufficient, so that the microprocessor 123 can process the second voltage signal to calculate a The sample magnitude; however, if the microprocessor 123 receives the second voltage signal and does not receive the first voltage signal, it means that the sample 2 does not cover the third identification film 1113, which also implies The first identification film 1111 and the second identification film 1112 may not be completely covered by the sample 2, and then the second voltage signal received by the microprocessor 123 may not be the complete first identification film 1111 and the second identification. As a result of the electrochemical reaction between the membrane 1112 and the sample 2, at this time, the specimen 2 must be injected onto the biosensor test piece 11, and the microprocessor 123 can convert the accurate sample through the complete electrochemical reaction. The display 124 is coupled to the microprocessor 123. The display 124 can be an LCD display or an LED display that can display the sample magnitude calculated by the microprocessor 123.

Please refer to FIG. 5 again, which is a circuit diagram of a first read circuit. The first read circuit 121 includes a first operational amplifier 1211, a first resistor R1 and a first analog/digital digit. The converter 1212, wherein the first operational amplifier 1211 has a first amplifier positive input terminal 121p, a first amplifier negative input terminal 121n and a first amplifier output terminal 121o, and the first amplifier positive input terminal 121p is coupled. Connected to a reference voltage Vref, the resistance value of the sensing code resistor Rs can be read through the first operational amplifier 121, and the electrochemical reaction on the third electrode 113 can be converted into the first voltage signal; The resistor R1 is coupled between the first amplifier negative input terminal 121n and the first amplifier output terminal 121o, and the first resistor R1 is used as one of the first operational amplifiers 121 to output a gain amplification resistor; the first analog/digital conversion The device 1212 is coupled to the first amplifier output terminal 121o to receive the first voltage signal and digitize it to obtain a first voltage value Vsf.

Please refer to the sixth figure again, which is a circuit diagram of a second read circuit. The second read circuit 122 includes a second operational amplifier 1221, a second resistor R2 and a second analog/digital digit. The converter 1222, wherein the second operational amplifier 1221 has a second amplifier positive input terminal 122p, a second amplifier negative input terminal 122n and a second amplifier output terminal 122o, and the second amplifier positive input terminal 122p is coupled. Connected to the reference voltage Vref, the electrochemical reaction on the first electrode 111 can be converted into the second voltage signal through the second operational amplifier 122; the second resistor R2 is coupled to the second amplifier negative input terminal 122n. The second resistor R2 is used as the output gain amplifying resistor of the second operational amplifier 122. The second analog/digital converter 1222 is coupled to the second amplifier output 122o for receiving. The second voltage signal is digitized to obtain a second voltage value VoutB.

Through the above description, it is possible to clearly understand the constituent elements and functions of the detectable test piece code and the sample filling device 1 which are insufficiently filled with the sample. Please refer to the third and fourth figures, and refer to the same. FIG. 7 is a circuit diagram of a first read circuit and a sense code resistor. In this embodiment, when the biosensor test piece 11 is inserted into the process display unit 12, the sample 2 has not been injected into the biosensor test. The first reading circuit 121 is electrically connected to the sensing code resistor Rs via the third electrode 113. As shown in the seventh figure, the sensing code resistor Rs is connected to the first amplifier. The negative input terminal 121n, therefore, the resistance value of the sensing code resistance Rs can be converted by the formula Vsf=(Vref/Rsc)×R1c+Vref, wherein Vsf is the first voltage value, Vref is the reference voltage, Rsc is the resistance value of the sense code resistor Rs, and R1c is the resistance value of the first resistor R1 (Vsf, Rsc, and R1c are symbols using a symbol instead of a component symbol).

Please refer to the third and fourth figures, and refer to the eighth figure at the same time, which is a circuit diagram of a first read circuit and a sense code resistance and an electrochemical reaction equivalent impedance, wherein when the amount is sufficient The sample 2 is injected into the biosensor test piece 11, and the sample 2 sequentially covers the first identification film 1111, the second identification film 1112 and the third identification film 1113 via a siphon phenomenon, and the second identification film 1112 and the third identification film 1113 will electrochemically react with the sample 2, and the first reading circuit 121 reads the result of the electrochemical reaction through the third electrode 1113, as shown in the eighth figure, at the first In the circuit diagram of the read circuit 121, one of the electrochemical reaction equivalent impedance R23 generated by the electrochemical reaction is connected in parallel with the sense code resistor Rs, and the electrochemical reaction equivalent impedance R23 is connected to the first amplifier. The negative input terminal 121n, therefore, the impedance value R23c of an electrochemical reaction equivalent impedance R23 can be converted by the formula Vsf=[Vref×(R23c+Rsc)]/[(R23c×Rsc)×R1c]+Vref. (Vsf, Rsc, R1c, and R23c use symbols instead of component symbols for the formula).

In addition, the present invention further includes a second embodiment, please refer to the ninth and tenth views at the same time, which is a structural diagram of a second embodiment of a bio-sensing device capable of detecting a test chip and a sample filling deficiency A top view of the second embodiment of the test strip, as shown in the ninth and tenth views, is the same as the first embodiment of the present invention, and in the second embodiment, includes a biosensor test strip 11 and a The display unit 12 is configured to include: a first electrode 111, a second electrode 112, a third electrode 113, a sample flow path 114, and a siphon air hole 115; The unit 12 includes a first reading circuit 121, a second reading circuit 122, a micro processing unit 123, and a display 124.

Continuing to refer to the ninth and tenth embodiments, different from the first embodiment of the present invention, in the second embodiment, the sensing code resistor Rs is not disposed on the biosensor test piece 11, but is added. The yoke electrode 116 is composed of a plurality of single electrodes 1161, and a scribe line is disposed between the adjacent single electrodes 1161. The dicing electrode can separate the adjacent single electrodes 1161. In addition, a plurality of sensing code resistors are added to the processing display unit 12, and the plurality of sensing code resistors are a first sensing code resistor Ra and a second sensing code resistor Rb. a third sensing code resistor Rc, a fourth sensing code resistor Rd, and a fifth sensing code resistor Re are connected in series, and the fifth sensing code resistor Re is connected in series with a grounding resistor Rgnd and grounded. The resistance value of the first sensing code resistance Ra, the second sensing code resistance Rb, the third sensing code resistance Rc, the fourth sensing code resistance Rd, and the fifth sensing code resistance Re The combination of the resistance values thereof may represent the test piece code of the biosensor test piece 11.

Please refer to the tenth figure, and refer to the eleventh and twelfth drawings at the same time, which is a top view of the finger electrode of the bio-sensing device capable of detecting the test piece code and the sample filling insufficient, and the first reading circuit thereof. An equivalent circuit diagram of the second embodiment. In the second embodiment, the cutting path between the adjacent single electrodes 1161 is a first cutting path 116a, a second cutting path 116b, and a third cutting. A track 116c, a fourth scribe line 116d, and a fifth scribe line 116e, wherein the purpose of setting the scribe line is to select an appropriate combination of resistance values to represent the test piece code. As shown in the eleventh and twelfth drawings, when the first scribe line 116a is cut, the separated single electrode 1161 and the body of the finger electrode 116 form an open state. The second sensing code resistor Rb, the third sensing code resistor Rc, the fourth sensing code resistor Rd, and the fifth sensing code resistor Re are directly grounded through the grounding resistor Rgnd; and the first One end of the sensing code resistor Ra is grounded through the body of the finger electrode 116 and the grounding resistor Rgnd. The other end of the first sensing code resistor Ra is coupled to the first resistor R1 and the negative input end 121n of the first amplifier. In this way, the resistance value of the first sensing code resistance Ra can be read out through the first reading circuit 121 to represent the test piece code; when the resistance value of the first sensing code resistance Ra is selected as the test piece code After that, in the second embodiment, in the second embodiment, the first reading circuit 121 can read the electrochemical reaction equivalent impedance R23 and output the first voltage value Vsf, and determine the first Whether or not the voltage value Vsf is output indicates whether the sample 2 is sufficiently filled.

The foregoing has completely and clearly disclosed the first embodiment and the second embodiment of the present invention. In summary, it can be seen that the present invention has the following advantages:

A first embodiment of the present invention, wherein the sensing code resistor is disposed on the biosensor test piece to represent the test piece code of the biosensor test piece, so that when the biosensor test piece is inserted into the process display unit The processing display unit can automatically read the test piece code, so that the user (the diabetic patient) can input the wrong test piece code, thereby causing the processing display unit to calculate the wrong sample size.

2. The first embodiment of the present invention, wherein the third identification film is added to the third electrode on the biosensor test piece, and the first identification film is used in the sample flow path. After sequentially covering the third identification film, the processing display unit can confirm that the inspection system has completely covered the first identification film and the second identification film by detecting the electrochemical reaction on the third electrode, thereby accurately calculating And the sample value of the sample is displayed.

3. The second embodiment of the present invention is characterized in that a finger electrode is added to the biosensor test piece, and the plurality of sensing code resistors are additionally added to the first read circuit, and the user can use the cutting path. Selecting a combination of appropriate sensing code resistors to calculate different test chip codes, and at the same time, the first reading circuit can read the electrochemical reaction equivalent impedance and output the first voltage value to determine the first Whether the voltage value is output or not, it is known whether the sample is filled sufficiently.

However, the detailed description of the present invention is intended to be illustrative of the preferred embodiments of the present invention, and is not intended to limit the scope of the invention. The patent scope of this case.

1. . . Biosensor device capable of detecting test chip code and insufficient sample filling

1'. . . Conventional blood glucose sensing device

10’. . . Processing display unit

11. . . Biological test strip

111. . . First electrode

1111. . . First identification film

1112. . . Second identification film

1113. . . Third identification film

112. . . Second electrode

113. . . Third electrode

114. . . Sample flow path

115. . . Siphon vent

116. . . Finger electrode

1161. . . Single electrode

116a. . . First cutting road

116b. . . Second cutting road

116c. . . Third cutting road

116d. . . Fourth cutting road

116e. . . Fifth cutting road

12. . . Processing display unit

12’. . . Blood sugar test strip

121. . . First read circuit

1211. . . First operational amplifier

1212. . . First analog/digital converter

121n. . . First amplifier negative input

121o. . . First amplifier output

121p. . . First amplifier positive input

122. . . Second read circuit

122’. . . Strip substrate

1221. . . Second operational amplifier

1221’. . . Electrode part

1222. . . Second analog/digital converter

1222’. . . Operating electrode

1224’. . . Corresponding electrode

1226’. . . First wire

1228’. . . Second wire

122n. . . Second amplifier negative input

122o. . . Second amplifier output

122p. . . Second amplifier positive input

123. . . Micro processing unit

124. . . monitor

124’. . . Reaction layer

126’. . . Spacer

128’. . . Cover

2. . . Sample

R1. . . First resistance

R2. . . Second resistance

R23. . . Electrochemical reaction equivalent impedance

Ra. . . First sense code resistance

Rb. . . Second sense code resistance

Rc. . . Third sense code resistance

Rd. . . Fourth sense code resistance

Re. . . Fifth sense code resistance

Rgnd. . . Grounding resistance

Rs. . . Sense code resistance

Vref. . . Reference voltage

VoutB. . . Second voltage value

Vsf. . . First voltage value

The first figure is a perspective view of a conventional blood glucose sensing device;

The second figure is an exploded view of one of the blood glucose sensing test pieces used in the conventional blood glucose sensing device;

The third figure is an architectural diagram of a first embodiment of a biosensing device capable of detecting a test piece code and insufficient filling of a sample according to the present invention;

The fourth figure is a top view of the first embodiment of the biosensor test piece which is one of the biosensing devices capable of detecting the test piece code and the sample filling insufficient;

The fifth figure is a circuit diagram of the first read circuit of one of the bio-sensing devices capable of detecting the test chip code and the sample filling;

The sixth figure is a circuit diagram of the second read circuit of one of the bio-sensing devices capable of detecting the test chip code and the sample filling insufficient;

The seventh figure is a circuit diagram of the first read circuit and a sense code resistor;

The eighth figure is a circuit diagram of the first reading circuit and the sensing code resistance and an electrochemical reaction equivalent impedance;

Figure 9 is a structural diagram of a second embodiment of a biosensing device capable of detecting a test piece code and insufficient filling of a sample;

The tenth figure is a top view of a second embodiment of the biosensor test piece of the biosensing device capable of detecting the test piece code and the sample filling insufficient;

The eleventh figure is a top view of the finger electrode of one of the biosensing devices capable of detecting the test piece code and the sample filling insufficient; and

The twelfth figure is an equivalent circuit diagram of the second embodiment of the first reading circuit of the biosensing device capable of detecting the test piece code and the sample filling insufficient.

11. . . Biological test strip

111. . . First electrode

1111. . . First identification film

1112. . . Second identification film

1113. . . Third identification film

112. . . Second electrode

113. . . Third electrode

114. . . Sample flow path

115. . . Siphon vent

121. . . First read circuit

1211. . . First operational amplifier

1212. . . First analog/digital converter

122. . . Second read circuit

1221. . . Second operational amplifier

1222. . . Second analog/digital converter

R1. . . First resistance

R2. . . Second resistance

Rs. . . Sense code resistance

Vref. . . Reference voltage

Claims (14)

A biosensing device capable of detecting a test chip and a lack of filling of a sample, comprising: a biosensor test piece, comprising: a first electrode electrically connected to an electronic circuit, the first electrode having a The first identification film can be electrochemically reacted with a sample; a second electrode electrically connected to a ground end, the second electrode having a second identification film for electrochemically reacting with the sample; The measuring resistance is connected to the second electrode at one end thereof, and the resistance value of the sensing code resistor can represent one of the biosensor test pieces; and the third electrode is connected to the other end of the sensing code resistor. The third electrode has a third identification film for electrochemically reacting with the sample, and the third electrode is connectable to the electronic circuit to transmit the result of electrochemical reaction between the third identification film and the sample to An electronic circuit; a sample flow channel is disposed on the first electrode, the second electrode and the third electrode, and the sample flow channel surrounds the first identification film, the second identification film and the third identification film When the sample is injected onto the biosensor test piece, it can be followed by the sample. Road sequentially Covering and reacting with the first identification film, the second identification film and the third identification film; and a siphon air hole disposed above the first electrode, the second electrode and the third electrode, and close to the third Identifying the film, the siphon air hole may utilize a siphon phenomenon, and accelerate the sample in the sample flow path to sequentially cover the first identification film, the second identification film and the third identification film; and a processing display unit Inserting a biosensor test piece to process the electrochemical reaction result of the sample and the identification film, the process display unit includes: a first read circuit electrically connected to the third electrode, when the sample has not been injected into the biosensor test In the case of a chip, the first reading circuit is electrically connected to the sensing code resistor through the third electrode to obtain the resistance value of the sensing code resistance, and when a sufficient amount of the sample is injected into the biosensor test piece, When the sample flow path is sequentially covered by the first identification film to the third identification film, the first reading circuit can detect the electrochemical reaction on the third electrode, and output a first voltage signal; and a second Reading circuit electrically connected to the first electrode When the sample has not been injected into a biosensor test sheet, the second reading circuit signal can not read any of the electrochemical reaction, and when the subject is injected biosensor test strip, the second read circuit can read An electrochemical reaction on the first electrode, which in turn outputs a second voltage signal; a microprocessor coupled to the first read circuit and the second read circuit, when the sample has not dripped into the biological sense When the test piece is used, the microprocessor can obtain the resistance value of the sensing code resistance through the first reading circuit, and obtain the test piece code of the biosensor test piece after the calculation, and when a sufficient amount of the sample is dropped After entering the biosensor test piece, the microprocessor can receive the first voltage signal and the second voltage signal. When the microprocessor receives the first voltage signal, it can confirm that the sample body completely covers the first identification film and The second identification film, the microprocessor can convert the sample value of the sample by processing the second voltage signal; and a display coupled to the microprocessor, the display can display the microprocessor The calculated sample size. The biosensing device of the detectable test piece code and the insufficient filling of the sample according to the first aspect of the invention, wherein the first electrode is a working electrode, and the second electrode is corresponding to the working electrode. a counter electrode, the third electrode acts as a sensing electrode to assist the processing display unit to detect the test chip code, and assists the processing display unit in detecting and determining whether the sample completely covers the first identification film and the first Two identification films. The bio-sensing device of the detectable test piece code and the sample filling insufficient according to the first aspect of the invention, wherein the first read circuit further comprises: a first operational amplifier having a first An amplifier input terminal, a first amplifier negative input terminal and a first amplifier output terminal, wherein the first amplifier positive input terminal is coupled to a reference voltage, and the sensing code resistor can be read through the first operational amplifier a resistance value, and the electrochemical reaction on the third electrode is converted into the first voltage signal; a first resistor is coupled between the negative input terminal of the first amplifier and the output end of the first amplifier The first resistor is used as one of the first operational amplifiers to output a gain amplifying resistor; and a first analog/digital converter is coupled to the first amplifier output to receive the first voltage signal and digitize it. The bio-sensing device of the detectable test piece code and the sample filling insufficient according to the first aspect of the invention, wherein the second read circuit further comprises: a second operational amplifier having a second An amplifier input terminal, a second amplifier negative input terminal and a second amplifier output terminal, wherein the second amplifier positive input terminal is coupled to a reference voltage, and the second operational amplifier can be used to transmit the first electrode Converting the electrochemical reaction into the second voltage signal; a second resistor is coupled between the negative input terminal of the second amplifier and the output terminal of the second amplifier, the second resistor is used as the output gain amplification resistor of the second operational amplifier; and a second analog/digital digit The converter is coupled to the output of the second amplifier to receive the second voltage signal and digitize it. A biosensing device capable of detecting a test piece code and a sample filling insufficient as described in claim 1, wherein the sample is blood. The biosensing device of the detectable test chip code and the sample filling insufficient according to claim 1, wherein the sensing code resistor can be replaced by a sensing code capacitor and a sensing code inductor. The capacitance value of the sensing code capacitor and the inductance value of the sensing code inductance can represent the test piece code. The biosensing device of the detectable test piece code and the insufficient filling of the sample according to the first aspect of the invention, wherein the first identification film and the second identification film are used for verifying the main body of the sample. The identification film is used to check whether the sample has completely covered the first identification film and the second identification film. A biosensing device capable of detecting a test chip and a lack of filling of a sample, comprising: a biosensor test piece, comprising: a first electrode electrically connected to an electronic circuit, the first electrode having a The first identification film can be electrochemically reacted with a sample; a second electrode electrically connected to a ground end, the second electrode having a second identification film for electrochemically reacting with the sample; and a third electrode having a third identification film and the sample Conducting an electrochemical reaction, and the third electrode is connectable to the electronic circuit to transmit the result of electrochemical reaction between the third identification film and the sample to the electronic circuit; a sample flow channel is disposed at the first electrode a second electrode and a third electrode, and the sample flow channel surrounds the first identification film, the second identification film and the third identification film, and when the sample is injected onto the biosensor test piece, the The sample flow channel sequentially covers the first identification film, the second identification film and the third identification film, and electrochemically reacts therewith; a siphon air hole is disposed on the first electrode, the second electrode and the third electrode Above, and close to the third identification film, the siphon air hole can utilize the siphon phenomenon to accelerate the sample in the sample flow path, sequentially covering the first identification film, the second identification film and the third identification film; a finger electrode, adjacent to the first electrode and the third electrode, and formed on An end portion of the biosensor test strip, the interdigital electrode is composed of a plurality of single electrode, where the electrodes of the adjacent single There is a cutting path between the adjacent ones for separating the single electrodes to open the circuit; and a processing display unit for inserting the biosensor test piece to process the sample and the identification film As a result of the electrochemical reaction, the processing display unit includes: a first reading circuit electrically connected to the third electrode, and a sufficient amount of the sample is injected into the biosensor test piece, and follows the sample flow path by the first identification When the film sequentially covers the third identification film, the first reading circuit can detect the electrochemical reaction on the third electrode and output a first voltage signal; and the second reading circuit can be electrically connected to the second reading circuit. a first electrode, after the sample is injected into the biosensor test piece, the second reading circuit can read the electrochemical reaction on the first electrode, thereby outputting a second voltage signal; a plurality of sensing code resistors, the plurality of senses The measurement resistances are connected in series with each other, and when the biosensor test piece is inserted into the processing display unit, two ends of each of the sensing code resistors are respectively coupled to one single electrode; and the sensing code resistors connected in series are coupled In the first read circuit, wherein After most of the single electrodes are separated, the first electrode may be read circuit reads the measured value of the combined resistance of the sense resistor code to represent one of a biosensor test strip sensing sensing code; a micro processing unit is coupled to the first reading circuit and the second reading circuit, and the microprocessor can obtain the resistance values of the plurality of sensing code resistors through the first reading circuit, and after the calculation Obtaining the test piece code of the biosensor test piece, and after a sufficient amount of the sample is dropped into the biosensor test piece, the microprocessor can receive the first voltage signal and the second voltage signal, when the microprocessor receives After the first voltage signal is obtained, it can be confirmed that the first identification film and the second identification film are completely covered by the sample, and the microprocessor can convert the sample value of the sample by processing the second voltage signal. And a display coupled to the microprocessor, the display can display the sample magnitude calculated by the microprocessor. The biosensing device of the detectable test piece code and the insufficient filling of the sample according to claim 9, wherein the first electrode is a working electrode, and the second electrode is corresponding to the working electrode. a counter electrode, the third electrode acts as a sensing electrode to assist the processing display unit to detect the test chip code, and assists the processing display unit in detecting and determining whether the sample completely covers the first identification film and the first Two identification films. The bio-sensing device of the detectable test piece code and the insufficient filling of the sample according to claim 9 , wherein the first reading circuit further comprises: A first operational amplifier has a first amplifier positive input terminal, a first amplifier negative input terminal and a first amplifier output terminal, wherein the first amplifier positive input terminal is coupled to a reference voltage through the first An operational amplifier can read the resistance value of the sensing code resistor, and convert the electrochemical reaction on the third electrode into the first voltage signal; a first resistor is coupled to the first amplifier negative Between the input end and the first amplifier output, the first resistor is used as one of the first operational amplifiers to output a gain amplification resistor; and a first analog/digital converter is coupled to the first amplifier output for receiving The first voltage signal is digitized. The bio-sensing device of the detectable test piece code and the sample filling insufficient according to claim 9, wherein the second read circuit further comprises: a second operational amplifier having a second An amplifier input terminal, a second amplifier negative input terminal and a second amplifier output terminal, wherein the second amplifier positive input terminal is coupled to a reference voltage, and the second operational amplifier can be used to transmit the first electrode The electrochemical reaction is converted into the second voltage signal; a second resistor is coupled between the negative input terminal of the second amplifier and the output terminal of the second amplifier, and the second resistor is used as the second operational amplifier Output gain amplification resistor; and A second analog/digital converter is coupled to the second amplifier output to receive the second voltage signal and digitize it. A biological sensing device capable of detecting a test piece code and a sample filling insufficient according to claim 9 of the patent application scope, wherein the sample is blood. The biosensing device of the detectable test chip and the insufficient filling of the sample according to claim 9 , wherein the sensing code resistor can be replaced by a sensing code capacitor and a sensing code inductor. The capacitance value of the sensing code capacitor and the inductance value of the sensing code inductance may represent the test piece code. The biosensing device of the detectable test piece code and the insufficient filling of the sample according to claim 9 , wherein the first identification film and the second identification film are used for verifying the main body of the sample. The identification film is used to check whether the sample has completely covered the first identification film and the second identification film.