TWI386216B - Cyanophyceae spirulina composition - Google Patents

Description

Cyanobacteria spirulina composition

The present invention relates to a spirulina composition having an effect of improving kidney function.

Phycocyanin is one of the photosynthetic pigments contained in most of the cyanobacteria spirulina, and since it is a water-soluble protein, it can be made into an aqueous solution. The aqueous solution of phycocyanin exhibits a bright blue color. The spirulina which is a bright blue color in the form of an aqueous solution is widely known as a main component of a natural coloring matter used for coloring a food such as a chewing gum or a popsicle. Further, since phycocyanin has the same structure as the bile pigment of human or animal, it is also known as a substance deeply related to the living body.

From the past, it has been reported that phycocyanin has a physiologically active action, especially a renal function improving effect. In the case of a phycocyanin-containing agent, a drug for preventing renal diseases such as a spirulina containing phycocyanin-containing spirulina or a cyanobacterial spirulina as an active ingredient has been proposed (see Patent Document 1).

The phycocyanin contained in the water-soluble liquid of the cyanobacteria or cyanobacteria spirulina usually contains about 2 to 4% of phycocyanin, but contains such cyanobacteria or cyanobacteria. As described above, the agent for preventing kidney disease as an active ingredient has a phycocyanin content of about 2 to 4% as described above, so that serum creatinine cannot be sufficiently reduced, and thus renal function cannot be sufficiently improved. The problem.

[Previous Technical Literature]

[Patent Document 1] Japanese Patent Laid-Open No. 1-316326

The present invention is directed to solving the above problems.

That is, the object of the present invention is to provide a cyanobacteria spirulina composition which further enhances the effect of renal function recovery.

In order to achieve the above object, the invention of claim 1 is a cyanobacterial spirulina composition comprising: cyanobacteria spirulina powder and phycocyanin extracted from the cyanobacteria spirulina as a main component Blue pigment of the ingredients.

Further, the invention of claim 2 is the cyanobacteria spirulina composition of claim 1, wherein the cyanobacteria spirulina powder has a content of the blue pigment of 6 to 100 parts by weight. 20 parts by weight.

According to the invention described in the first aspect of the patent application, a cyanobacteria spirulina composition containing a cyanobacteria spirulina powder and a blue pigment containing phycocyanin extracted from the cyanobacteria spirulina as a main component is prepared. Therefore, it is possible to provide a cyanobacterial spirulina composition which further enhances the effect of renal function recovery. Regarding the content of the blue pigment, the cyanobacteria spirulina powder is preferably 6 to 20 parts by weight per 100 parts by weight.

The phycocyanin has a tetrapyrrole ring opened by a ring having a porphyrin ring The structure is called a structure of a water-soluble protein in which a pigment portion of phycocyanobilin and a protein are peptide-bound.

The person who has the greatest influence on the properties of phycocyanin is the part of the protein that occupies most of the molecule. Therefore, phycocyanin has the property as a protein, so phycocyanin has strength and weakness as a protein.

Since phycocyanin absorbs red light, its aqueous solution appears blue. Further, phycocyanin is a fluorescent substance that emits red fluorescence having a maximum absorption wavelength of 618 nm.

The phycocyanin usually forms a trimer or a hexamer. The single system is composed of α and β polypeptides, but in the case of phycocyanin, since α=16,000 and β=20,000, the molecular weight is estimated to be about 110,000 or 220,000. Further, since it is an acidic protein having an isoelectric point of 4.3, its aqueous solution is unstable under alkaline conditions, but is stable at pH 5 to 7.

In spirulina, in addition to phycocyanin, phycoerythrin and allophycocyanin are also included as phycobilin proteins, and the absorption curve actually overlaps these three peaks.

The phycocyanin is rapidly dissolved in cold water or warm water of 40 ° C or lower to form a bright blue aqueous solution. The solubility per 100 g of water is about 10 g. The phycocyanin is insoluble in an organic solvent such as ethanol, acetone or ether. The reason for this is that the protein is precipitated by denaturation of an organic solvent such as ethanol, acetone or ether. However, phycocyanin dissolves in an aqueous solution of 20% or less in ethanol, but is unstable and gradually precipitates.

When phycocyanin is an aqueous solution, its color will rapidly increase above 40 °C. It is darkened, but when it is a powder (water content is 8% or less), it does not change even if it is heated at 110 ° C for 2 hours. Further, when the phycocyanin is an aqueous solution, it is more stable on the acidic side than on the alkaline side, and generally, the fading rate for light is large.

A blue pigment having phycocyanin as a main component is produced in the following steps: (1) a step of immersing spray-dried spirulina in water to obtain a phycocyanin extract by extracting phycocyanin (2) a step of separating and removing the water-insoluble portion of spirulina from the phycocyanin extract using a centrifugal separator; (3) separating and removing the spirulina using an ultrafiltration membrane The phycocyanin extract which is insoluble in water is subjected to molecular weight separation, and the step of separating and removing the water-soluble portion having a molecular weight of 15,000 or less; (4) from the phycocyanin extract from which the water-soluble portion having a molecular weight of 15,000 or less has been separated and removed a step of separating phycocyanin; (5) a step of concentrating the separated phycocyanin as an ultrafiltration membrane; (6) adding an auxiliary material to the concentrated phycocyanin, by such A step of spray-drying to obtain a powdery blue coloring matter having phycocyanin as a main component; and (7) a step of sterilizing the powdery blue coloring matter using a hot air dryer.

The cyanobacteria spirulina composition of the present invention comprises: a cyanobacteria spirulina powder and a phycocyanin extracted from the aforementioned cyanobacteria spirulina as a main component Blue pigment. Regarding the content of the blue pigment, the cyanobacteria spirulina powder is preferably 6 to 20 parts by weight per 100 parts by weight.

As described in Examples 1 to 10 to be described later, the cyanobacteria spirulina composition of the present invention contains a cyanobacteria spirulina powder and a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component. The effect of restoring kidney function. However, when the amount of the blue coloring matter containing the phycocyanin extracted from the spirulina as a main component is less than 6 parts by weight, the amount of the cyanobacteria spirulina powder is less than 6 parts by weight, as will be described later in Examples 9 to 10. It is shown that the normal value of serum creatinine concentration in healthy men cannot be lowered: in the range of 0.60 to 1.20 mg/dl, but compared with 100 parts by weight of spirulina powder of cyanobacteria, the phycocyanin extracted by the aforementioned spirulina is mainly used. When the amount of the blue pigment added to the component is 6 to 20 parts by weight, the serum creatinine concentration (mg/dl) after 7 days of administration of the tablet can be lowered to a healthy male as shown in Examples 1 to 8 to be described later. Normal value of serum creatinine concentration: in the range of 0.60 to 1.20 mg/dl. In addition, the amount of the blue pigment added as the main component of the phycocyanin extracted by the spirulina may be reduced to the serum creatinine of a healthy male even if it is more than 20 parts by weight based on 100 parts by weight of the spirulina powder of the cyanobacteria. The normal value of the concentration is in the range of 0.60 to 1.20 mg/dl, but the blue pigment having the phycocyanin as a main component is expensive and expensive, and the manufacturing cost of the cyanobacterial spirulina composition is increased, which is less preferable. . Therefore, the content of the blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is preferably in the range of 6 to 20 parts by weight based on 100 parts by weight of the spirulina powder of the cyanobacteria.

Thus, when the cyanobacteria spirulina powder is contained in an amount of 100 parts by weight, By using 6 to 20 parts by weight of the blue pigment having phycocyanin extracted from the cyanobacteria as the main component, it is possible to provide a cyanobacterial spirulina composition which can improve the recovery of kidney function without increasing the manufacturing cost.

The cyanobacteria spirulina composition of the present invention is formed into a powder, granule or capsule to form a pharmaceutical product, but the cyanobacteria spirulina composition of the present invention is preferably tableted by a tableting machine to form a tablet. .

(Example)

The embodiments of the present invention are described below, but the present invention is not limited to the embodiments.

(Example 1)

To 100 parts by weight of the cyanobacteria spirulina powder, 6 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 1 below:

Also, the normal value of serum creatinine concentration in healthy men is 0.60. 1.20 mg/dl (if the serum creatinine concentration exceeds 2.50 mg/dl, dialysis is required).

(Example 2)

To 100 parts by weight of the cyanobacteria spirulina powder, 8 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 2 below:

(Example 3)

To 100 parts by weight of the cyanobacteria spirulina powder, 10 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 3 below:

(Example 4)

To 100 parts by weight of the cyanobacteria spirulina powder, 12 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 4 below:

(Example 5)

To a spirulina spirulina powder, 14 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to form a cyanobacteria spirulina composition, and the cyanobacteria is spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 5 below:

(Example 6)

To 100 parts by weight of the cyanobacteria spirulina powder, 16 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 6 below:

(Example 7)

To 100 parts by weight of the cyanobacteria spirulina powder, 18 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component is added to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 7 below:

(Example 8)

20 parts by weight of a blue pigment containing phycocyanin extracted from the cyanobacteria spirulina as a main component is added to 100 parts by weight of the cyanobacteria spirulina powder to prepare a cyanobacteria spirulina composition, and the cyanobacteria is spiraled The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 8 below:

(Example 9)

To 100 parts by weight of the cyanobacteria spirulina powder, 0.1 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component was added to prepare a cyanobacteria spirulina composition, and the cyanobacteria was spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 9 below:

(Embodiment 10)

To 100 parts by weight of the cyanobacteria spirulina powder, 5.0 parts by weight of a blue pigment having phycocyanin extracted from the cyanobacteria spirulina as a main component was added to prepare a cyanobacteria spirulina composition, and the cyanobacteria was spiraled. The algae composition is tableted by a tableting machine to form a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 10 below:

(Comparative Example 1)

A cyanobacterial spirulina composition composed only of cyanobacteria spirulina powder was tableted by a tableting machine to prepare a tablet. Then, the thus-obtained tablet was administered to 10 male patients having a serum creatinine concentration of 2.50 mg/dl for 20 days per day for 20 days (0.2 g/pound x 20 tablets), and the serum creatinine concentration was measured every day. The measurement results are shown in Table 11 below:

The relationship between the "days after the administration of the tablets of the tablet" and the "serum creatinine concentration (mg/dl)" described in Examples 1 to 10 and Comparative Example 1 Shown in Figure 1. In Fig. 1, the horizontal axis represents the number of days (days) after administration of the tablet, and the vertical axis represents serum creatinine concentration (mg/dl). In addition, the relationship between the amount of the blue pigment added (parts by weight) in the tablet and the serum creatinine concentration (mg/dl after 7 days) described in Examples 1 to 10 and Comparative Example 1 is shown in the first section. 2 picture. In Fig. 2, the horizontal axis represents the amount of blue pigment added (parts by weight), and the vertical axis represents the serum creatinine concentration (mg/dl) after 7 days.

In Examples 1 to 8, 6 parts by weight, 8 parts by weight, 10 parts by weight, 12 parts by weight, and 14 parts by weight of the blue coloring matter containing phycocyanin as a main component were added to 100 parts by weight of the cyanobacteria spirulina powder, respectively. Ingots of 16 parts by weight, 18 parts by weight, and 20 parts by weight, and Examples 9 and 10 were prepared by adding 0.1 parts by weight and 5.0 parts by weight of a blue pigment having phycocyanin as a main component. In the comparative example 1, a tablet containing a blue pigment having phycocyanin as a main component was added, but the following matter can be seen from Fig. 1. That is, in Examples 1 to 8, the serum creatinine concentration (mg/dl) was rapidly decreased until 7 days after the administration of the tablet, and in comparison with Examples 9, 10 and Comparative Example 1, serum was used. The creatinine concentration (mg/dl) was only slowly decreased until 7 days after the administration of the tablet. However, Examples 9 and 10 containing a cyanobacteria spirulina powder and a blue pigment containing phycocyanin extracted from the cyanobacteria spirulina as a main component were compared with Comparative Example 1 not containing the blue pigment. The slope of the serum creatinine concentration (mg/dl) is large. Further, as shown in Fig. 2, in Examples 1 to 8, the serum creatinine concentration (mg/dl) after 7 days from the administration of the tablet was lowered to a normal value of serum creatinine concentration in healthy men: 0.60 to 1.20. The range of mg/dl is compared to the blood of healthy men in Examples 9, 10 and Comparative Example 1. The normal value of the clear creatinine concentration is in the range of 0.60 to 1.20 mg/dl.

Fig. 1 is a graph showing the relationship between the days after the administration of the tablets of the tablets obtained in Examples 1 to 10 and Comparative Example 1 and the serum creatinine concentration (mg/dl).

Fig. 2 is a graph showing the relationship between the amount (parts by weight) of the blue dye added to the tablets obtained in Examples 1 to 10 and Comparative Example 1 and the serum creatinine concentration (mg/dl) after 7 days.

Since the picture in this case is experimental data, it is not a representative figure of this case.

Therefore, there is no designated representative map in this case.

Claims (2)

A cyanobacterial spirulina composition comprising: a cyanobacteria spirulina powder and a blue pigment having a phycocyanin extracted from the cyanobacteria spirulina as a main component. The cyanobacterial spirulina composition according to claim 1, wherein the cyanobacteria spirulina powder has a content of the blue pigment of 6 to 20 parts by weight per 100 parts by weight.