TWI333420B - Vapor fraction from seeds of glycine max (l.) merr. and composition thereof - Google Patents

Description

1333420 玖, the invention description: [Technical field to which the invention pertains] The present invention relates to a vapor component obtained from soybean (G/>;e/ne followed by (L) 乂(10)), which has a treatment for skin damage It treats skin diseases, stimulates cell regeneration and stimulates hair growth. [Prior Art] Soybean (wa; c (L.) Merr) contains soybeans and black beans, which are among the world's most important sources of oil and protein. For example, soy can be processed into an edible semi-drying oil which can be used as a salad oil or as a margarine and ghee. Soybean oil is also used in the manufacture of coatings, linoleum, tarpaulins, inks, soaps, insecticides and disinfectants. In addition, lecithin phosphate obtained from by-products of the oil industry can be used as a wetting agent and stabilizer in foods, cosmetics, pharmaceuticals, leathers, paints, plastics, fertilizers and detergents. Soy flour is a protein-rich feed for livestock. In addition, soy protein can be used to make synthetic fiber 'adhesives, fabric sizing, waterproofing, fire fighting foam, and the like. In medical use, soybean has been reported to have an effect on many diseases. Large Dan can be used as a nutrient for regulating the function of the intestines, heart, kidneys, liver and stomach. Since large oil contains a large amount of unsaturated fatty acids, it can be used for paraoxane cholesterol. The medical lecithin obtained from soybean can be used as an anti-fatty liver agent. In addition, it is known that a steroid as a primary anti-hardening factor and a sterol which is a substitute for diosgenin in some antihypertensive drugs can be prepared from soybean. Isoflavones and plant-estrogens found in soybeans have been shown to prevent various cancers including breast, prostate and colon cancers. See "Plants, Hormones and Cancer" (AdlercreuU, Η;

ΟΛ83\83756 DOC 1333420

Phyto-oestrogens and cancer. The Lancet Oncology, 2002 » Vol_ 3 ' p· 364-3 73 ) ? We also found that the consumption of phyto-taste with phyto-male can reduce total plasma cholesterol in middle-aged and elderly patients with hypercholesterolemia And the concentration of LDL-cholesterol, see "Eating a dose of soy phytosterol-added ground beef to reduce serum total cholesterol and LDL cholesterol in male and young hypercholesterolemia patients" (Matvienko, 〇. A., Lewis) , DS, Swanson, M., Aendt, B., Rainwater, DL, Stewart, J., and Aleke1, D. L.; A Single daily dose of soybean phytosterols in ground beef, serum total cholesterol and LDL cholesterol in young, Mildly hypercholesterolemic men. dw A/^r·, 2002, 76, p. 57-64). Certain soy extracts have also been reported to have a pharmaceutical effect. The i,i-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical scavenging activity from the 70% aqueous acetone extract of the brown soybean variety (Akita-Zairai) seed coat is revealed in "the brown soybean seed coat has a high Highly polymerized procyanidins in brown soybean seed coat with high free radical scavenging activity (Takahata. Y., 〇_-Kameyama, M_, Furuta, S·, Takahashi, and Μ., Suda, I.; a high radical-scavenging activity. J. Jgric. F(10)d C/iew·, 2001, 49, p. 5843-5847). It was found that extracts from germ extract, large denier, rice bran, tear grass, sesame, wheat, fragrant oak, green tea, green leaf extract and germinated rice bran were below 6 〇. (: After an electric baking, slowly baking and using Aspergillus oryzae for more than 3 days to fertilize each component into a low molecular weight substance, it can have an antioxidant effect, see "Antioxidant effect of processed cereals" ( Minamiyama, Y., Yoshikawa, O:\83\83756.DOC -6- 1333420 T·, Tanigawa, T·, Takahashi, S·, Naito, Y., Ichikawa, Η.

Kondo, M.; Antioxidative effects of a processed grain food. /·Sb·Sci. i994, 49;·Pan: #6W7).J Carrot, X. __ _ ' . . *· Words of flowers and black beans Beans including red beans, mung beans and soybeans; · · · · Leaf boiling extracts have strong anti-caries promoter and free radical scavenging activity, see "lipid peroxide free radicals and tumor-promoters High correlation between effects: inhibition of tumor proliferation in EBV/B-lymphocyte systems by various plant extracts and removal of alkyl peroxide free radicals" (Maeda.

Correlation.,Katsuki,T·,Akaike,Τ· and Yasutake. R.; High correlation between lipid radical and tumor-promoter effect: suppression of tumor promotion in the Epstein-Barr virus/B-lymphocyte system and scavenging of alkyl peroxide Radicals by various vegetable extracts. Jpn. J. Cancer Res., 1 992, 83, p. 923-928). By measuring serum glutamate-oxalic acid-transaminase (sGOT) and serum proline-pyruvate-transaminase (sGPT) in rats

The active, black bean water extract also showed an effect on liver disease caused by acetaminophen ‘see “Evaluation of hepatoprotective activity against legumes” (Wu, S.-J.,

Wang, J.-S. and Chang C.-H·; Evaluation of hepatoprotective activity of legumes. Phytomedicine, 2001, V〇l. 8(3), p. 2 1 3-2 1 9 ) ° A soybean seed extract obtained by an extract composed of a mixed system of an aliphatic alcohol and a low-boiling ester ester is shown as a beverage preparation (Japanese Patent No. 1 117767). It has been found that certain specific soy extracts can be used in cosmetics or medicines for the treatment of certain skin conditions.

O: \83\83756 DOC 1333420 A soy extract containing a predetermined ratio of sphingomyelin and phospholipids is disclosed for use in the treatment of dry skin cosmetics (U.S. Patent Publication No. US 2002/00095 09 A1). These extracts can be produced by extracting mature whole soybean or oil-free soy flour using an aliphatic alcohol alone or a mixture thereof with water, and then treating with an aliphatic hydrocarbon and an aliphatic ketone. Therefore, the extract is fat-soluble.

It has been found that one contains one or more plant extracts selected from the group consisting of whey, and a cortex extract, and further selected from the group consisting of scutellaria, sage, and soybean (G/less wax (L.) Merrill) The cosmetic composition, the acne medicine, or the blackhead acne production inhibitor of the various extracts is effective for preventing or treating a skin disease such as acne or irritated skin caused by acne (Japanese Patent No. 2001097842). The composition can be used to make regular beverages, foods, and the like.

For example, propylene glycol isoflavones can be used as an epithelial cell proliferation promoting agent (Japanese Patent No. 9〇59166). This glucoside was obtained by the following procedure: #45-65. (: The diced soybean was immersed in a water towel adjusted to pH 7·5 to ρΗ9·0 with a caustic surface for 2.4 hours, and the large water was removed to obtain the water extract as the water extract 'Using the ultrafiltration membrane to remove from the extract The protein is obtained as a filtrate, and the filtrate is contacted with an adsorbent and the sugar bitter is eluted from the adsorbent f by using an aqueous solution of an alcohol or an aqueous solution of an alcohol. The product can also be used as an anti-, lingual sputum group, reagent, food, cosmetics and medicine (°). For example, Japanese Patent No. 4139132

O:\83\83756.DOC 1333420 However, there is no prior art to give or clarify the low-concentration alcohol or alcohol-free vapor fraction of soybeans (a (L.) Merr·) that are easy to prepare for the treatment of skin damage and skin. The use of the disease. SUMMARY OF THE INVENTION The present invention provides a smectic (max (L.) Merr.) seed to obtain a scorpion gas fraction which has a significant effect on treating skin damage, treating skin diseases, stimulating cell regeneration, and stimulating hair growth. The vapor fraction of the present invention is prepared by a process comprising the steps of: (a) providing a crude extract of the seed in an alcohol solution (containing an alcohol having a concentration of less than about i5% by weight) or water; and (b) At atmospheric pressures below about 1 atm and temperatures below about 11 〇. The crude extract is evaporated under the underarm to obtain a vapor fraction. This vapor fraction is different from any known soy (G/L (M) Merr.) seed extract because it is displayed as a unique graphic by high performance liquid chromatography () fingerprinting. Specifically, a Waters Sphers 〇rbTM-〇DS2 column having an inner diameter (丨D) of 4.6 mm, a length (L) of 25 mm, and a particle size of 5 μm was obtained at a wavelength of 20 〇 nanometers. The HPLC chart of the sulphur vapor column includes a deviation coefficient of 8% and a hysteresis time of 2.910, 5.190, 13.190 and 50.815 minutes, wherein the mobile phase is 95% 020/5°/❶CH3CN at 0 to 5 minutes, at 5 The gradient to 30 minutes is 95% H20/5% CH3CN, the gradient from 30 to 80 minutes is 75% H20/25% CH3CN, and the gradient at 80 to 1 minute is 〇% H2〇/l〇〇% CH3CN The gradient from 1 〇〇 to 1 minute is 〇% H20/1〇〇% CH3CN' and 95% H2〇/5% CH3CN at 101 minutes;

ΟΛ83\83756 〇〇C 1333420 and the flow rate is 1 ml/min. The invention also provides a composition comprising the vapor fraction of the invention. In particular, the composition can be used in a pharmaceutical composition, a cosmetic composition or a skin cleansing composition. [Embodiment] According to the present invention, we have unexpectedly discovered soybeans (Likely like (L)

Men·.) The Qiqi Branch has a significant effect on the treatment of skin damage, treatment of skin diseases, stimulation of cell regeneration and stimulation of hair growth. According to the present invention, a seed from soybean (ca-(4); c (L.) Meir.) is known to be produced by a method comprising the following steps: (a) providing a solution in an alcohol solution (which contains a concentration of less than about i5 % w of alcohol or a crude extract in water; and (b) evaporation at atmospheric pressure below about i _ and at a temperature below about

The crude extract is taken to obtain a vapor fraction. ...X We have unexpectedly found that the vapor fraction obtained from the crude extract of the seed in an alcohol solution (which contains an alcohol in a concentration of about % wt) or in water has a dermatological effect. This vapor fraction is easy to prepare. From beans (Cai (8) wgML·) Merr.) Seed preparation medicines or chemical products are very profitable. '(1) Soybeans can be eaten for a long time and are believed to be non-toxic; and (2 soybeans are inexpensive. Soy (_ (L) alcohol The liquid (which contains a concentration lower than that commonly used in the art, can be used to practice the invention. For example, in one embodiment of the invention, Merr.) the crude extract of the seed is used - about 5% wt Obtained by alcohol or water extraction. Any method for obtaining a crude extract of the seed is O:\83\837J6.DOC -10- 1333420 For the sake of 'the crude extract can be mixed by any conventional method, such as grinding' The perturbation, cutting or chopping method divides the seed into pieces and immerses it in an alcohol solution having a concentration lower than 1 sc / 15 wt. In a preferred embodiment, the alcohol/minus is lower than About 5% by weight, more preferably less than about (four) or about wt. According to the invention, the crude extract may also be extracted with water. In a preferred embodiment, the seed pieces are immersed in by weight. 5% to about 10% of the "alcohol or water." This technique is commonly used to separate seeds and extract the divided seeds. Any of the ways can be used to practice the invention. According to this month, the far coarse extract has an atmospheric dust force of less than about 1 atm and a temperature of less than about U 〇 ° C (preferably between about 60. (: to about 11) The underarm is vaporized. The vapor fraction can be collected in liquid form by cooling the vapor. In a preferred embodiment of the invention, the hair is raised at a given atmospheric pressure and /m degrees And collecting the vapor fraction by cooling vapor can be carried out in a rotary evaporator, wherein the vapor is evaporated into a condenser provided with cold water, and then the vapor is cooled through the condenser and collected in liquid form The steam fraction is simple and inexpensive. The vapor fraction according to the present invention can be analyzed and evaluated by high performance liquid chromatography (HPLC). For example, an inner diameter (ID) of 4.6 mm and a length are used. L) The HPLC profile of a 50 μl vapor fraction of a Waters SpherS〇rbTM-〇DS2 column of 250 mm and a particle size of 5 μm at a wavelength of 200 nm includes a deviation factor of 8% and a hysteresis time of 2· 91〇, 5.190, 丨3.19〇 and 5〇, 815 Peak of the peak, where the mobile phase is 95% H20/5% CH3CN for 0 to 5 minutes; 95% H2〇/5% cH3CN for a gradient of 5 to 30 minutes, 75 OV83\83756 for a gradient of 30 to 80 minutes .doc -11- % H2〇/25% CH3CN; the gradient at 80 to 100 minutes is 〇% H2O/100% Ch3CN; the gradient at 100 to 1〇1 minute is 〇% H2O/100% ch3CN, at 101 The minutes are 95% H20/5 % c:H3 (:N, and the mud speed is 1 ml/min. According to the map, the substance in the vapor fraction shows a strong polarity. It differs significantly from the other components disclosed in the prior art. For example, there are four rings in the structure of daidzein and genistein which have a weak polarity. In a preferred embodiment of the invention, several herbs may be added to aid in the treatment of skin lesions, stimulating cell regeneration and stimulating hair growth by treating soybean (max (L.) Men··) seeds. According to the present invention, a gas fraction is self-contained with soybean seeds and contains at least one seed selected from the group consisting of sesame seeds, green p (corpse l·) seeds, and The substances in the group consisting of antibacterial herbs are obtained from raw materials. Preparation of Vapor Fraction from Raw Material and Analysis and Identification The vapor fraction is as described above. Preferably, the antibacterial herb is selected from the group consisting of dried rhizome of rhubarb (L.), dried root of Rhei Rhiz〇ma, dried bark of oyster (), and dried bark of Phellodendd Cortex. Group. On the other hand, the raw material preferably contains from about 40% to about 100% of soybeans ((7) less than ... err) seed, 0 to about 50% of sesame (μ山·c_) seeds, 0 to about 50% of mung beans (corpse six prints / L) seeds and sputum to about 30% of antibacterial herbs. In a more preferred embodiment of the invention, the raw material comprises about 7% of the large denier ((4) Eu (.) Merc.) seeds, about 2% of the sesame seeds.

0\83\83756.DOC -12· 1333420 (Sesamumindicum) seeds, about 5% of Rheumpalmatum L. dried rhizome or rhubarb. (RheiRhizoma) dried rhizome and about 5% of jaundice (Phellodendron amurense Rupr) .) Dry tree: Dry bark of Phellodendri Cortex. This material is in about 2% alcohol. In another preferred embodiment of the present invention, the raw material comprises about 8% of soybeans (L.) Men*.) and about 2% of mung beans (for example, (9) / (10) μ mountain L. )seed. The feedstock is in about 2% alcohol. In still another more preferred embodiment of the present invention, the raw material comprises about 8% by weight of soybean (L.) Meir.) seeds and about 2% by weight of sesame (ζ·„山·cww) seeds. In about 2% of the alcohol. In another preferred embodiment of the invention, the raw material comprises about 90% of soybean (L_) Men··) seeds and about 1% of rhubarb (and office (9) Dry rhizome of rhesus rhubarb or rhubarb (Rhei Rhiz〇ma). The feedstock is in water. In another preferred embodiment of the invention, the feedstock comprises about 8% soy (waML. Merr) seeds, about 1% sesame (Sesam (indicative) (dic)) and dried rhizome of about 1% rhubarb (Rhe_ corpse / aiwm L.) or dried rhizome of rheusia (Rhei Rhiz〇ma). In the preferred embodiment of the present invention, the raw material contains about 53% of large denier ((10)e m(n:(L) Merr) seeds of about 2% by weight of sesame (heart 5_ pre-species, About mung bean (corpse W▲ (10) coffee L.) seeds about 5% of rhubarb (Rheum palmatum L) dry rhizome or rhubarb

ON83\83756.DOC -13- 1333420 (Rhei Rhizoma) dry stem and about 5% of the dried bark of arnwrewe and wpr. or the dry bark of PhellodendriCortex. The feedstock is in about 3% or about 2% alcohol or water. Moreover, the HPLC profile of a 50 μl vapor fraction obtained using a Waters SphersorbTM-ODS2 column having an inner diameter (ID) of 4.6 mm, a length (L) of 250 mm and a particle size of 5 μm at a wavelength of 200 nm includes deviation The coefficient is 8% and the lag time is 2.896, 5. m, 12.944 and 46.603 minutes 'peak' where the mobile phase is 95% H20/5% CH3CN in 〇 to 5 minutes, '* gradient in 5 to 30 minutes is 95 % H20/5% CH3CN, gradient from 30 to 80 minutes, 75% H20/25% CH3CN, gradient from 80 to 100 minutes, 0% H20/100% CH3CN, gradient from 1〇〇 to 1〇1 minute It is 〇% H2O/100% CH3CN, 95% H20/5% CH3CN at 101 minutes, and the flow rate is 1 ml/min.

The invention also provides a composition comprising a vapor fraction according to the invention. Preferably, the composition is selected from the group consisting of - a pharmaceutical composition - a cosmetic composition and a skin cleansing composition. In a preferred embodiment according to the present invention, the composition further comprises an antibacterial preparation, such as borneol. Preferably, the amount of vapor (four) according to the present invention is between about 70% of the total weight of the composition. Between about 80% and a specific embodiment of the invention from %^' the composition further comprises borneol, stearic acid, stearyl alcohol, palmitic acid, ^ Λ 1 ^, cetyl alcohol, beeswax and poly Oxygen extended ethyl sorbitan monostearate r pr^, u 0 曰l Ρ〇Ε) to form an ointment that is easy to apply to the subject. The method comprises administering a sufficient amount of the skin lesion, treating the skin, stimulating cell regeneration and stimulating hair growth, which can be effectively treated for treating 4 skin lesions of the skin or skin diseases 0'83'83756 D〇c • 14-1333420 A composition according to the invention. The vapor fraction according to the present invention can be used to treat skin damage, skin diseases, stimulate cell regeneration, and stimulate hair growth. For example, skin lesions or skin diseases include burns, sunburns, light shots, acute and chronic, erythema, dermatitis, spots, chujin, nodules, blisters, large blisters, pustules, and rubella. Plaque, cyst, scaly, sputum, fissure, ulcer, acne, dry skin, peeling, itching, allergic wet therapy, treatment and hair follicle defects. Preferably, the composition according to the present invention is effective for treating burns, light shots, acute and sexual injuries, dermatitis, gangrene, chronic therapeutic ulceration, abrasions, atopic dermatitis, suture wounds, diabetic wounds and hair follicle defects. . In the animal model described below, it has been demonstrated that the vaporous copper component according to the present invention is effective for treating large areas of skin defects, scars and allergic reactions without granulation. In addition, the hair follicle defect can be treated and the hair can grow again without leaving erythema or burnout. The speed and condition of healing of wounds treated with the vapor sub-sector according to the present invention are better than those of the conventional medical treatments. When the wound is healed according to the present invention <vaporation, the skin color of the wound has been the same as that of the normal skin. Further, in the histological examination, the use of the skin tissue of the wound according to the present invention does not significantly cause the appearance of a large damage and requires complicated reconstruction surgery for fiber denaturation. Further, after the treatment of the wound treated with the vapor fraction of the present invention, no allergic reaction, inflammation, pustule or rash was observed, and the growth of the accessory organs and cells of the wound was the same as that in the normal skin. In contrast, a conventionally treated O:\83\83756.DOC •15-1333420 medically treated wound has a smaller number of accessory organs and cells than normal skin and the wound has a greater fiber width. • This & 疋Vapor fractionation according to the invention is effective in treating radiation injuries. So far, there are no drugs that can effectively treat occupational injuries. We have unexpectedly found that in the treated subjects, the wounds treated with the vapor branch showed lower hair damage and no allergic reactions. Further, inflammation and tissue contracture which are usually observed when the wound is healed are not present in the wound using the vaporous branch according to the present invention; The vapor fraction is effective for treating wounds with damaged skin. Thus, the present invention provides a preferred, convenient and economical means for treating large areas and skin wounds. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention. Example 1: Preparation of Soybean (Glycine max (l.) Merr.) Seed Vapor Fractions Grinding Packings i (G7 less che max (L.) Merr.) seeds, sesame (hAcwm) seeds, mung beans (corpse Aase) 〇& •L.) l.) dried rhizome of seeds, rhubarb (Rose eww pa/waiwm L.) or dried rhizome of rhubarb (Rhei Rhizoma) and dried bark or Phellodendron (Phell〇dendri Cortex.) The raw material of the dried bark is extracted with 8% by weight of alcohol or distilled water to obtain a crude extract. Its content is shown in Table 1. The vapor fraction is evaporated in a rotary evaporator (EYELA N-1000S, 1000 S-W) at a pressure of less than 1 atm and at a temperature of 60 ° C and passed through a condenser provided with cold water. obtain.

〇\83\83756 DOC -16· 1333420 Table 1 Raw material branch number '1 2 3 4 5 6 7 8 Soybean (max (L,) Merr.) Seed (%) 100 70 53 80 0 80 90 80 Chiba!^ (Sesamum indicum) Seed (%) 0 20 20 0 0 20 0 10 (Phaseolus radiatus L·) Seed (%) 0 0 17 20 0 0 0 0 Rhubarb (Rheumpalmatum L·) of dried rhizome or rhubarb (Rhei Rhizoma Dried rhizome (./〇) 0 5 5 0 0 0 10 10 Special: medicine l iPhellodendron amwr hunger dry bark or dried bark of Phellodendri Cortex (%) 0 5 5 0 0 0 0 0 solution distilled water ( %) 98 98 98 98 100 98 100 100" Alcohol (%) 2 2 2 2 0 2 0 0 The HPLC results of 50 μl fraction 1 are shown in Figure 1. The HPLC was carried out using a Waters SphersorbTM-ODS2 column with an inner diameter (ID) of 4·6 mm, a length (L) of 250 mm and a particle size of 5 μm, wherein the mobile phase was 95% H20 at 0 to 5 minutes. /5% CH3CN, 95% H20/5% CH3CN in 5 to 30 minutes, 75% H20/25% CH3CN in 30 to 80 minutes, 0% H20/100 in 80 to 100 minutes % CH3CN, gradient from 1 〇〇 to 1 〇 1 min is 〇% H20/100% CH3CN '95% H20/5% CH3CN at 1〇1 min and the flow rate is 1 ml/min. The HPLC was carried out using a Waters Alliance 2795 HT and the spectrum was taken using a Waters Photo Diode Array 2996 at a wavelength of 190 to 400 nm. As shown by the spectrum obtained at the wavelength of 200 nm, there is a sharp symmetry when the deviation coefficient is 8% and the lag time is 2.910, 5.190, 13.190 and 50.815 minutes. 〇 \83\83756 DOC -17· 1333420 The HPLC results of 50 μl of fraction 3 are shown in Figure 2. The HPLC was carried out using a Waters SphersorbTM-ODS2 column with an inner diameter (ID) of 4.6 mm, a length (L) of 250 mm and a particle size of 5 microns, wherein the mobile phase was 95% H20 for 0 to 5 minutes. /5% CH3CN, 95% H20/5% CH3CN in 5 to 3 minutes, 75% H20/25% CH3CN in 30 to 80 minutes, 〇% H20/ in 80 to 100 minutes 1〇〇% CH3CN, the gradient from 100 to 101 minutes is 〇% H2〇/i〇〇% CH3CN, 95% H20/5% CH3CN at 101 minutes, and the flow rate is 1 ml/min. The HPLC was performed using a Waters Alliance 2795 HT and the spectra were taken using a waters Photo Diode Array 2996 at a wavelength of 190 to 400 nm. As shown by the map at a wavelength of 200 nm, there is a spike at a deviation factor of 8% and hysteresis times of 2.896, 5.111, 12.944, and 46.603 minutes. Example 2: Preparation of ointment The ointment according to the present invention is as exemplified in Table 2: Table 2 Content (wt) Distillate Τ7~%~ Borneol 0.6% _ Stearic acid 5% -_Stearyl alcohol 4% __Brown Acid 4% - hexadecanol 5% Beeswax 2% Oxygen extended ethyl sorbitan monostearate 2.4% The ointment of the present invention may use any of the conventional methods commonly used in the art.

OV83\83756.DOC •18· Method preparation. Example 3. Effect on total cortical injury and mouth of rabbits Treatment of animals: New Zealand adult female white rabbit weighing 2.9 to 3.1 kg was used as animal turmeric. The mother two rabbits were individually kept in the pot and the silver was mixed with feed and water. Rabbits were anesthetized with 25 to 40 mg/kg chloramine hydrochloride. The back of each rabbit was completely sheared and cut off 6 inches; ·] In addition to 6 areas < full cortex (each area is UX1.5 cm ^ 2), where the whole cortex contains the epidermis, dermis and meat film. Dosage and frequency: The wounds of 6 areas per rabbit were treated twice daily with cartilage containing fractions i to 6 twice daily. The ointments are applied topically to the wound in an amount of 0_3 5 g/cm 2 . Clinical Wound Evaluation: The mother-day observation wound was photographed three times a week using a reference standard under a uniform illumination, focal length, and aperture conditions to record the wound healing process. In addition, the size of the wound was also measured and is shown in Table 3. Table 3 The wound area treated with ointment containing the following number of the following items 1 2 3 4 5 6 0 100.0 100.0 100.0 100.0 1000~~~ 100 0 2 105.5 103.1 103.8 105.1 Ϊ209~~~ —117.3 ___130.0 —96.0 55.1 -204 ~~~_ 〇-5 5 108.9 110.2 105.5 100.4 ^ 1344~~~ 8 80.0 72.9 78.3 75.3 9Ϊ0 11 49.4 47.1 49.4 39.2 54Ό 14 17.9 15.1 17.0 16.5 2L0 17 7.7 6.2 9.4 9.1 Ϊ02~~~ ~74 — ~U-- 19 3.6 4.1 4.1 4.4 22 0.5 0.5 0.0 0.5 25 0.0 0.0 0.0 0.0 0?^ The results indicate complete healing of the wound (as shown in Figure 3). According to the present invention, 〇 \S3\83756 DOC -19-1333420 agent (ointment containing fraction 5) hair growth evaluation: ... is effective for treating whole cortical wounds. Measure the length of the new hair. The average length of the hair using all the wounds containing the points of the object is ... centimeters (Fig. 2... The average length of the hair in the untreated area is Gi^2: eight, and the result shows that the vapor fraction according to the present invention The remainder can be repaired: 2. It stimulates hair growth. In addition, the shape according to the invention: long-acting effect. t-enhanced hair Example 4: effect on rat whole cortical wounds Treatment of animals: from National Cheng Kung University of Taiwan Sprague-Dawley adult male rats were purchased from the Experimental Animal Center. Rats weighing 300 to 350 g were anesthetized with 25 to 4 mg/kg ketamine hydrochloride. The back of each rat was thoroughly sheared using an electric shearing machine. The rats were individually housed in cages and fed with mixed feed and water. For each rat, a full skin layer of 2 5 χ 2 5 cm 2 was cut, including the epidermis, dermis and sarcolemma, see Β. Hafemann et al., "Use of a collagen/elastin-membrane for the tissue engineering of dermis. Burns. 1999; 25: 373-384" and H.-J. Wang Waiter "Using a pig dermal template to enhance the growth of thin, self-contained, semi-thick skin of a wide-spread mesh" (Use of a Porcine Dermis)

Template to Enhance Widely Expanded Mesh Autologus Split-Thickness Skin Graft Growth. J Trauma. 1997 Feb;42(2):177-182).

0: 'β3'83756 DOC • 20-1333420 Dose and frequency: Two experimental groups of rats were treated with an ointment containing sub-packages 7 and 8 as shown in Example 2, and each group contained 3 rats. The pharmaceutical composition is applied topically to the wound in an amount of 35 g / cm 2 and a thickness of 0.1 to 0.2 cm. All wounds were wrapped with sterile gauze and elastic bandages, and the kits were replaced daily. The evaluation methods used were similar to those of Example 3, and the wound size was also measured and shown in Table 4. Day _ use the package accounted for the following, the flat number: 7? The sound of the soft sound treatment of the jealousy and guess (%, 7 8 '~~~ 0 100 100 —' --- 2 85.3 102.5 5 64.3 71.4 — 9 24 34.5 12 18.6 25.2 15 9.3 13.4 - 17 3.1 8.4 20 0.4 2.5 23 0.0 0.0 Wound healing treated with an ointment containing fractions 7 and 8.

The wound area treated with the pharmaceutical composition comprising fraction 7 is reduced more rapidly. Example 5: Effect on deep wound healing of deep second-degree burns Treatment of animals: • Purchased from the Experimental Animal Center of Mutual Success University of Taiwan.

Sprague-Dawley adult female rats. Nine rats weighing 230 to 280 g were anesthetized with 25 to 4 mg/kg of salicylamine. Use a electric shearing machine to cut completely in one of the predetermined wound areas in the middle of the back of each rat.

〇AS3'83756 DOC 1333420 毛. Each rat was individually housed in a cage and fed with a mixed feed and water. Use a method such as that described by Walker and Mason to produce burn wounds in rats and rats. See ZR. Gao et al., "Use of pig skin collagen as a wound dressing for skin donor sites and deep second degree burns" (Porcine dermal collagen). 1992; 18(6): 492-496), wherein the surface of the back skin is designed to produce a deep second degree burn of the skin having an area of 4×4 cm 2 . The template was exposed to 75 ° C water for 15 seconds. Dosage and frequency: The experimental group (G3) of the rat was treated with the pharmaceutical composition according to the present invention, which contained a fraction 9 similar to Fraction 3, except that the alcohol content was as shown in Example 2. 3%. The rat control group (G1) was not treated and the rat comparison group (G2) was treated with silver sulfadiazine drug (produced by SWISS PHARMACEUTICAL CO., LTD.). Each group contained 3 rats. The drug was applied topically to the wound in an amount of 0.35 g/cm 2 twice a week until the hair grew. The clinical wounds were evaluated. The evaluation methods used were similar to those of Example 3, and the wound size was measured and shown in Table 5. Table 5 Day G1 G2 G3 Wound area (mm 2 ) Change in initial area (%) Wound area (mm 2 ) Change in initial area (%) Wound area (mm 2 ) Change in initial area (%) 1 1260.00 100.00 1140.67 100.00 1776.33 100.00 5 938.67 74.50 1053.33 92.34 744.00 41.88 11 285.33 22.65 428.00 37.52 154.67 8.71 14 188.00 14.92 234.67 20.57 34.67 1.95 0 V83V83756 DOC -22- -___ G ~7^---------- Day 18 Wound area ( Square mm) ----- 〇〇n Change in initial area (%) VJ^ Wound area (mm 2 ) Initial change in knowledge f%) G. Wound area (mm 2 ) 3 Change in initial area (ΟΑΛ 2Γ 31 0Ό0 ' 2^〇__ 0.00 141.33 "64.67 12.39 5 67 0.00 (/〇) 0.00 〇t ___0.00 _ 0.00 0.00 41.33 32.37 ------1 —Srj U.U0 0.00 0.00 0.00 0.00 0.00 The wound treated by the ointment of the present invention is: no symptoms, and the skin color is the same as the normal skin color. However, (4) the silver drug treatment has a symptom on the wound. In addition, the large silver drug heals at 18 days' It is earlier than them Use the conventional amines = therapist (which still has 2.86% of the wound area on the 37th day). The following == number;: round record. New hair growth fraction erythema; "new hair growth" and has - Some of the scorch and new "new hair growth' have some erythema; "〇" refers to the new hair growth of the wound area of the new hair. The scores of the clip growth are listed in Table 6:

〇: New hair growth of gamma 3756 〇〇C -23- 1333420. No erythema or eschar was found in the wound treated with the pharmaceutical composition according to the present invention. However, no new hair growth was observed when treated with conventional drugs. Histological evaluation of the skin: After the wound was completely healed, the skin tissue of each rat was taken and anesthetized to perform histological examination using an evaluation method as described by B. Hafemann et al. 'See B. Hafemann et al. Use of a collagen/elastin-membrane f〇r the tissue engineering of dermis. Burns. 1999; 25: 373-384). The sample was taken and stored in formaldehyde. Each sample was embedded in a stone soil closure and thin sections were prepared and stained using the hematoxylin-eosin method. The samples were observed under an optical microscope and a transmission electron microscope. The results of histological examination showed that the untreated large oxygen epithelial tissue (as shown in Fig. 5) was uneven in thickness and the granular layer became thick. The fibrous layer can be observed in the deep cortex. The uneven skin epithelial tissue was also shown in the rat group (shown in Figure 6) treated with a conventional amine π-bite silver drug. The fibrous layer can be observed in the deep cortex and the hair follicles are destroyed. On the other hand, the wound treated with the ointment according to the present invention (as shown in Fig. 7) appears to be normal, and a large amount of hair follicles are regenerated. The fibrous layer can be observed in the surface area of the skin. Example 6: Effect on large-area whole cortical wounds in rats Treatment of animals: Sprague-Dawley adult male rats were spoken from the Experimental Animal Center of National Cheng Kung University in Taiwan. Rats weighing 300 to 350 grams were anesthetized with 25 to 40 mg/kg of amiodarone. The back of each rat was O:\83\83756 D0C -24· 1333420 Thoroughly cut with an electric shearing machine. Each rat was individually housed in a cage and fed with mixed feed and water. For each rat, a full skin layer of 4.0 X 4.0 cm2 was cut, wherein the whole skin layer contained the epidermis, dermis, and meat membrane. Dosage and frequency: The experimental group of rats was treated with a soft paste containing fractionated product 9 as shown in Example 5. The present invention also provides a comparative group (G2) of a rat treated with a silver sulfadiazine drug (produced by SWISS PHARMACEUTICAL CO., LTD.). Each group contained 5 rats. The ointment was applied topically to the wound in an amount of 0.75 g/cm 2 and a thickness of 0.1 to 0.2 cm. All wounds were wrapped with sterile gauze and elastic bandages and the dressing was changed daily. The evaluation methods used were similar to those used in Example 3, and the wound size was also measured and shown in Table 7. Table 7 Change of initial area of wound area of ointment α-bite silver drug containing fraction 3; change of initial area of wound area (mm 2 ) (%) (mm 2 ) (%) 1 1977.60 100.00 1957.60 100.00 3 1763.20 89.16 1784.80 91.17 5 1444.80 73.06 1506.40 76.95 7 990.40 50.08 1265.60 64.65 9 556.00 28.11 768.80 39.27 11 429.60 21.72 456.80 23.33 13 368.80 18.65 385.60 19.70 15 283.20 14.32 299.20 15.28 17 208.80 10.56 236.80 12.10 19 166.00 8.39 238.40 12.18 21 131.00 6.62 161.60 8.26 23 119.00 6.02 123.20 6.29 25 87.00 4.40 88.00 4.50 27 45.00 2.28 76.00 3.88 29 40.00 2.02 56.00 2.86 31 35.00 1.77 34.40 1.76 35 18.00 0.91 20.00 1.02 33 16.00 0.81 8.00 0.41 37 0.00 0.00 0.00 0.00 〇\83\83756 DOC -25 · In the process of wound healing Among them, the wound treated with the ointment according to the present invention can be an allergic reaction. However, scars can be formed on wounds treated with silver sulfadiazine. In addition, severe allergic reactions were observed in wounds treated with silver sulfadiazine. The recovery rate (about 25 to 31 days) of the rats treated with the pharmaceutical composition according to the present invention was faster than those treated with the silver sulfadiazine drug (about 37 days). Histological Evaluation of Skin: The method used for histological evaluation was as described in Example 5. The histological examination performed on the 45th day showed that the fibrous layer could be observed in the deep cortex of the wound treated with the conventional sulfadiazine silver drug. Some epithelial cells die and form more. Chronic inflammation and opiate, ''X,'' field penetration can be observed in the skin. Some fibers can also be formed 5 cm from the wound. In the group of rats treated with the ointment according to the present invention, the neonatal epithelium was flat and a large number of hair follicles were produced. Only a small amount of fiber is formed. Histological examinations performed on day 95 indicated that an uneven epithelial layer (as shown in Figure 8) was observed on wounds treated with conventional sulfonamides. The fibrous layer is formed in the deep cortex. A small amount of hair follicles are produced. In the group of rats treated with the ointment according to the present invention, the neonatal epithelium was flat and a large number of hair follicles were produced (as shown in Fig. 9). Example 7: Effect on local electron radiation skin damage Treatment of animals · ptague-Dawley adult female rats were purchased from the Experimental Animal Center of National Cheng Kung University, Taiwan. Rats weighing 25 to 3 grams

〇 \83\83756 D〇C •26- 1333420 Separately in cages and fed with mixed feed and water. Rats were subjected to light shots using methods similar to those described by John E. Moulder and James J. Fischer, see "Light-Effect Reaction of Rat Skin: Effect of Number of Fractions and Total Treatment Time" (RADIATION REACTION OF) RAT SKIN: The role of the Number of

Fractions and the Overall Treatment Time. Cancer June 1976; 37(6): 2762-2767). Rats were anesthetized with 40 to 50 mg/kg pentobarbital sodium salt (TCI. TOKYO) in the rat peritoneum before irradiation. The skin on the gluteal region is completely cut and marked on the right side of the expected 2X2 square centimeter of radiation area and on the left gluteal muscle before irradiation. Irradiation was carried out by applying an electron beam of 6 MeV energy generated by a linear accelerator (manufactured by Varian Associates, Inc., CA, U.S.A.). Test fractionation and single dose irradiation "In fractional irradiation, the right and left gluteal muscle regions of 3 rats were irradiated. The irradiation was performed twice a week for five weeks and the total dose on each area was 37.5 Gy.

Dosage and frequency:

The radiation injury after daily irradiation at a dose of 0.25 g/cm 2 in fractional irradiation was treated with an ointment containing fractionation 9 (G1) as shown in Example 5, and the radiation injury per single dose irradiation was Two treatments were performed on the day. Untreated rats were used as controls (G2). A blank group (G3) of the rats that did not receive the irradiation was used as a blank. Clinical evaluation'· Evaluate and record the skin reaction scores 30 days after irradiation, see Yoshinao Abe and Muneyasu Urano “After multiple daily doses of Ο 83 \83\83756 DOC -27-1333520 part of the mouse Fraction side-dependent Acute Skin Reaction of Mice after Multiple Twice-A-Day Doses. Int. J. Radiation Oncology Biol. Phys. 1990 Feb; 18(2): 359-364) and Yu- Jen Chen et al., "The Effect of Tetrandrine and Extracts of Centella asiatica on Acute Radiation Dermatitis in Rats. Biol. Pharm. Bull. 1999 July; 22(7): 703-706). The skin reaction score is defined as follows: “〇, 'refers to normal skin; “〇· 5 ” means that less than 50% of the irradiated areas have slight hair loss' or hair discoloration; “1.0” means that about 50% of the irradiated areas are depilated. "1.5" means that more than 50% of the irradiated areas are depilated and some of the depilation is accompanied by redness of the skin; "2.0" means that less than 50% of the irradiated areas are completely depilated and dry peeled; "2.5" means more than 50% Dry peeling occurred in the irradiated area; “3.〇” means some wet peeling; “3.5” means wet peeling in most areas. The skin reaction score was evaluated and as shown in Table 8.

O:\83\83756.DOC -28- 1333420 Skin did not show an allergic reaction. This result is similar to those in the fractional exposure test. Histological evaluation of skin: The evaluation methods used were similar to those used in Example 5. In G1 (as shown in Figure 1A), epidermal cells were found to coat multiple layers of epithelial cells. A large number of hair follicles are also regenerated. In G2 (as shown in Figure 11), only a small number of hair follicles were observed. In G3 (as shown in Figure 12), the tissue served as a control. The treated wound tissue is denser, but they are more slack without treatment. Example 8: Effect on dermatitis of rabbit ears & As shown in Fig. 13, it was found that dermatitis of rabbit ears has symptoms of congestion, swelling, inflammation, ulceration and bleeding. The ointment according to the present invention was applied to the wound for 3 days, and it contained a fraction 10' similar to the copper fraction 3, except that the alcohol content was as % as shown in Example 2. On the first day, the ulcer disappeared and the congestion was reduced. Hair follicles appear as shown in Figure 14. On day 2, skin tissue recovered and only a slight redness was observed. The hair follicles are clearly visible, as shown in Figure 15. On the third day, the skin tissue and color were normal. Hair grows on the hair follicles, as shown in Figure 16. Example 9: Effect on Diabetes Trauma A 68-year-old man has diabetes for 20 years. Its left toe has been removed. The wound showed severe gangrene changes, as shown in Figure 17. All the toes of its right foot have

O:\83\83756.DOC -29· 1333420 is shown in Figure 18. Apply to the wound, as shown in Figure 19, without more necrosis. 2 to 3 times. It can be noticed that some meat is removed but the wound is not healed. The ointment containing the fraction 9 will be treated for 2 months (the bud tissue, and the wound begins to heal the left foot for 5 months. r α _ _ . After treatment (as shown in Figure 20), the wound healed completely without leaving a scar. The right foot (as shown in Figure 2) was completely healed without scarring. Example 10: Chronic hoarding The role of the ulcer is shown in Figure 22. One in eight men, the old man and the lower extremities suffer from hyperpigmentation and satiety therapy for 20 years. Apply the ointment containing fraction 9 to the wound, daily 2 Up to 3 times. After 2 weeks of treatment of the foot t (as shown in Figure 23), the wound begins to heal. Foot, work 4 weeks / after σ treatment (as shown in Figure 24), the wound is completely cured and does not leave bubbles Example 11: Effect on Ramadan Female patients have ecchymosis caused by a car accident (as shown in Figure 25). The wound is pre-treated for three days with a long cold compress, and then administered 2 to 3 times a day. The ointment containing the fraction 9. As shown in Fig. 26, the ecchymosis disappears and the wound heals on the third day after the treatment. The car accident 刖 already exists < The marks and colors also flattened and became lighter after 7 days of treatment (as shown in Figure 27). Example 12: Effect on abrasions As shown in Figure 28, - female patients suffered from skin abrasions caused by car accidents. The ointment of 9 is applied to the wound 2 to 3 times a day. The wound heals after 7 days of treatment (as shown in Figure 29). O:\83\83756 DOC -30· 1333420 Example 13: Effect on suture wounds Male patients with severe tear wounds, it is in the middle of the female and female, "Gang 3 Gong split mouth and 20 stitches suture, the nose bridge has a 公 8 male split mouth H count suture and the upper lip has 1 split and 6 needles The suture and 丨5 cm to the A knife mouth and the 8 needle suture. Apply the ointment containing the fraction 9 to the wound, 2 days until the wound is completely healed without leaving scars (as shown in Figure 3). Example 14: Effect on atopic dermatitis A 4.5-year-old girl suffers from chronic atopic dermatitis. As shown in Figure 31, after long-term topical corticosteroid treatment, the skin of the neck and upper chest is itchy, congested, and swollen. , splitting, oozing, crusting and scaly. Up to 3 times., swelling, cracking will contain fractions ίο soft The cream is applied to the wound, after 2 weeks of treatment per day (as shown in Figure 32), pain, congestion, exudation, scarring and scaling are reduced and weakened. Skin damage almost subsides. Skin damage completely heals and follows After 4 weeks of treatment (as shown in Fig. 3), after 6 weeks of treatment (as shown in Fig. 34), there is no rebound effect. Although the specific embodiment I of the present invention has been explained and described, the heat Tf is The artist can still implement various modifications and improvements. From L. g. 7 for a long time (and therefore, the present invention has been embodied for the purpose of illustration only, and is not intended to limit the invention. The invention is not

In the specific form set forth, T, 杳 & A 且 and without contradicting the subject matter and scope of the present invention are modified within the scope defined by the accompanying application. .Simple description of the schema]

Figure 1 shows the sub-brows obtained by the method according to the invention! 〇^83\83756 〇〇C -31 - 1333420 lb. 20 to 4 minutes; Mingzhi ointment treatment of whole cortex HPLC map; Fig. la ·· 0 to 20 minutes; Fig. 1 c · 40 to 60 minutes. Figure 2b: 20 to 4 minutes; Figure 2 shows the HPLC spectrum according to the invention; Figure 2a: 0 to 20 minutes; Figure 2c - 40 to 60 minutes. Fig. 3 shows a photograph of the wound according to the present invention used in Example 3. The treatment of the whole skin to 3 soft Figure 4 is not used in Example 3 using the ointment according to the invention: hair growth photos on the wound; W 4a: containing the remnant: 霄, Figure 4b: containing the sub-district 4 to 6 Ointment. Fig. 5 shows the results of the non-learning test in Example 5. The treatment of the deep second degree burn wound tissue The deep second degree of drug treatment The clear ointment treatment of deep second degree The large area of drug treatment Figure 6 shows the histological examination results of the sulfadiazine silver wound in Example 5. Fig. 7 shows the results of histological examination using the burn wound according to the present invention in Example 5. Figure 8 shows the results of histological examination of the sulfadiazine silver skin wound in Example 6. Cream Treatment Large Area Figure 9 shows the results of histological examination of the soft whole cortical wound according to the present invention in Example 6. The figure shows the results of histological examination using the skin lesion according to the present invention in Example 7. 〜~therapy light shot Figure U shows the results of the untreated transfer test in Example 7. And the histology of stomach injury

O: \83\83756.DOC -32 - 1333420 Figure 12 shows the results of normal tissue in Example 7. ΙΓ shows the smear of the rabbit ear dermatitis before the treatment of τ in Example 8. 4 The photograph of the first day after the dermatitis according to the present invention was used in Example 8. Further ointment treatment ear-free seat Fig. 15 shows a photograph of the second day after the use of the skin inflammation in Example 8. Further treatment of rabbit ears Fig. 16 to 6 shows the photograph of the third day after the use of the hair follicle in Example 8. a &lt;soft poor treatment of rabbit ear seat Figure 17 - ^ Example 9 diabetes wound Figure 1 S - ~ ', H piece. In the case of the diabetes in Example 9, Q + , ..., 芍. A photograph of the use of the wound according to the present invention for 2 months after the use of the present invention is not shown. Λ 敕 治疗 治疗 治疗 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图The human ointment was used to treat diabetes. The figure shows the photograph of the use of the spring wound according to the present invention for 5 months. Human θ treatment of diabetes Fig. 22 shows the prevention of chronic sagittal ulceration in Example 1 (). Fig. 23 shows a photograph of the use of the tablet/creulous ulcer according to the present invention in Example 10 after 2 weeks. <Cholesterol treatment chronic Fig. 24 shows a photograph of the use of the cancer in accordance with the present invention in Example 1 for 4 weeks. Human Bone Treatment Chront Figure 25 shows a photograph of Freckle in Example 11. Fig. 26 shows a photograph of the use of the day according to the present invention in the example. Human cream treatment of Ramadan 3

O: \83\83756 DOC -33 - 1333420 Figure 27 shows a photograph of the treatment of ecchymosis using the ointment according to the present invention in Example 11 for 7 days. Figure 28 shows a photograph of the scratch in Example 12. Figure 29 shows a photograph of the treatment of ecchymosis 7 days after the use of the ointment according to the present invention in Example 12. Figure 30 is a photograph showing the treatment of a suture wound using the ointment according to the present invention in Example 13. Figure 31 shows a photograph of atopic dermatitis in Example 14. Fig. 32 is a photograph showing the treatment of atopic dermatitis 1 week after the use of the ointment according to the present invention in Example 14. Fig. 33 is a photograph showing the treatment of atopic dermatitis 4 weeks after the use of the ointment according to the present invention in Example 14. Figure 34 is a photograph showing the treatment of atopic dermatitis in Example 14 using the ointment according to the present invention for 6 weeks. O:\83\83756 DOC -34-

Claims (1)

1333420 》" Patent Application No. 092122224 Replacement of Chinese Patent Application (May 1994) Pick up and apply for patent garden: 1. A kind of seed obtained from soybean (G/_ychema; c(L.) Merr.) a vapor fraction, which is prepared by a process comprising the steps of: (a) providing a crude extract of the seed in an alcoholic solution containing less than about 15% by weight of alcohol or water; (b) evaporating the crude extract at an atmospheric pressure of less than about 1 atm and a temperature of less than about 110 ° C to obtain the vapor fraction; wherein the inner diameter (ID) is 4.6 mm and the length (L) is 250 mm. And a 50 μl vapor fraction obtained by high performance liquid chromatography (HPLC) at a wavelength of 200 nm, with a particle size of 5 μm, including a shift factor of 8% and a hysteresis time of 2.910. The peaks of 5.190, 13.190 and 50.815 minutes, wherein the mobile phase is 95% H20/5% CH3CN for 0 to 5 minutes, 95% H20/5% CH3CN for 5 to 30 minutes, and a gradient of 30 to 80 minutes. 75% H20/25°/〇CH3CN, with a gradient of 80% to 100 minutes at 0% H20/100% CH3CN, at The gradient from 100 to 101 minutes was 0% H20/100°/〇 CH3CN, 95% H20/5% CH3CNf at 101 minutes, and the flow rate was 1 ml/min. 2. The vapor fraction of claim 1 wherein the vapor fraction is collected in liquid form by cooling vapor. 3. The vapor fraction of claim 1 wherein the concentration of the alcohol is less than about 5% wt. 4. The vapor fraction of claim 1 wherein the crude extract in step (a) is in water. 83756-940530.doc 1333420 5. The vapor fraction of claim 1, wherein the temperature in step (b) is between about 60 ° C and about 110 ° C. 6. For a patented range of g1 g of steam fraction 16, it has the treatment of skin damage, treatment of skin diseases, stimulation of cell regeneration and stimulation of hair growth.炙乍 7. A composition comprising a vapor fraction as described in the patent application. Goshen Patent | The composition of item 7, which is used in the manufacture of a pharmaceutical group, a cosmetic composition or a skin cleansing composition. 9. The composition of claim 7, which is for use in the treatment of skin damage or skin disorders. 10. The composition of claim 9, wherein the skin damage or skin condition is selected from the group consisting of burns, sunburns, radiation injuries, acute and chronic injuries, ecchymoses, dermatitis, spots, papules, nodules, blisters, large Blister, pustule, rubella, plaque, cyst, scaly disease, sputum, fissure, ulcer 'acne, dry skin, peeling, itching, allergic eczema, rash, gangrene' chronic hoarding A group of abrasions, atopic dermatitis, suture wounds, diabetic wounds, and hair follicle defects. 11. The composition of claim 9, wherein the skin damage or skin condition is selected from the group consisting of burns, radiation injuries, acute and chronic injuries, dermatitis, gangrene, chronic hoarding ulcers, abrasions, atopic dermatitis, Suture wounds, diabetic wounds, and hair follicle defects. 12. The composition of claim 7, further comprising an antibacterial agent. 3 'A composition as claimed in claim 7, which further comprises borneol, 83756-940530.doc 1333420 stearic acid, stearyl alcohol, palmitic acid, cetyl alcohol, beeswax and polyoxyethylene Sorbitan monostearate. A pharmaceutical composition for treating skin damage or skin diseases of a patient, comprising a composition effective for treating skin damage, treating skin diseases, stimulating cell regeneration, and stimulating hair growth, as in the composition of claim 7 . 83756-940530.doc