1328424 99年4月27日修正替換 九、發明說明： 【發明所屬之技術領域】 本發明是有關於一種栽培菇類的培養基配方，且特別 是有關於一種以鋪地黍栽培菇類的培養基配方。 【先前技術】 全球菇類產業在近幾十年來蓬勃發展，菇類產量逐年 提升，由於菇類大部分是屬於木生菌，其主要的培養基為 闊葉樹木屑’需要相當的林木資源來滿足菇類產業的需 要，因此菇類產業的發展也衍生出菇類生產與林業生態平 衡的”菌林矛盾”問題’菇類產業也勢必隨著森林面積的逐 漸縮減’而面臨此一產業無法持續發展的困境。 目前改善的方法是以菇類培養廢料部份取代木屑培 養基來栽培其他益類，同時發展菌草取代木屬來栽培益類 的技術。所謂菌草即是經科學實驗證明適合用於栽培食、 藥用菇類的草本植物，目前已經形成另一種產業一菌草 業’菌草業的發展也相對的舒緩了人口、食物、資源、環 境、能源等問題。 側耳屬（尸spp.)是全世界產量第三高的菇類， 此屬的菌絲生長速度快，分解纖維素、木質素能力強。秀 珍兹（P/和金頂側耳（朽⑼ ⑽咖叩心加奶）為側耳屬的菇類，在台灣秀珍菇是產量居 第四大的菌種，金頂側耳則是近年來新興的菌種，因其容 易培養且美味可口，所以頗受栽培業者和消費者的喜愛。 5 1328424 99年4月27日修正替換 過去相關的報告曾指出不僅草本、木本植物均可做為 側耳屬菇類的生長基質，農業廢棄物或其他木質纖維成分 亦可做為生長基質。相較於一般木屑培養基，若以適當的 菇類廢棄生長基質或草本植物部份取代木屑栽種，可達到 提尚其產量的效果。 【發明内容】 因此本發明就是在提供一種以鋪地黍栽培菇類的培 養基配方’用以達到提高側耳屬菇類產量的目的。 根據本發明之實施例，提出一種栽培菇類的培養基配 方。包含35%重量比的乾料部分及65%重量比的水，其中 乾料部分包含30〜60%重量比之鋪地黍草桿、3〇〜60%重量 比之木屑' 9%重量比之米糠以及1%重量比之碳酸約。培 養基配方的整體含水量約為65%左右》 本發明之栽培菇類的培養基配方，利用野生草本植物 鋪地黍部份取代木屑做為培養基配方，在菌絲生長速度和 生物效率皆較傳統僅使用木屑做為培養基之配方提高，可 有效達到增加側耳屬菇類產量的目的。 【實施方式】 本發明實施例以秀珍菇、金頂側耳為例，觀察以鋪地 黍部份取代木屑做為培養基配方的菌絲生長速度、子實體 產量及生物效率。 依照本發明之實施例，試驗菌株（秀珍菇及金頂側耳） 6 1328424 99年4月27日修正替換 係保存於4它冰箱。進行試驗時，將菌絲塊接種到馬鈴薯 葡萄糖洋菜培養基（PDA)培養皿，在25°C培養箱活化，經 再一次活化後的菌株，即可將菌絲塊接種於麥粒培養基。 麥粒培養基之製備係先將小麥以水洗淨，置於水中過夜， • 濾除水分後，加入1%米糠及0.5%碳酸鈣，混合均勻後每 500克裝入塑膠菌種瓶’於高溫高壓蒸氣鋼（12TC，1.5 kg/cm2)滅菌80分鐘後冷卻即可使用。 φ 接種菌絲塊後的麥粒培養基置於25eC培養箱培養，在 培養過程中不定時的搖動以避免麥粒結塊，14天後菌絲長 滿麥粒表面，此即為麥粒菌種。 鋪地黍代料係將野外或田間收集的鋪地黍 π/^似）以粉碎機粉碎，過篩收集長度為2公分以下的草 桿，曬乾後裝袋備用。 將已堆積至少三個月之闊葉樹雜木屑（山黃麻、楠 木、相思木、鳳凰木）與鋪地黍代料混合，製成數種不同混 φ 合比例的培養基配方，培養基的含水量皆調整在65%左 右。將混合均勻之培養基每1100克裝入太空包塑膠袋，經 1.5 kg/cm2、12PC滅菌80分鐘，冷卻後，將麥粒菌種接入 不同配方的培養基。接菌後的培養基置於乃它的培養室， 不照光下進行菌絲培養，相對濕度控制在7〇 75%。 其中，秀珍菇菌絲長滿太空包後，經34天將太空包 移至18°C下刺激2天後，溫度調回25t：，並進行割包， 兹蕾產生後相對濕度控制在9G%以上，使子實體繼續成 長’直至適當大小即可採收。 7 1328424 ---— 99年4月27日修正替換 金頂側耳培養至原基體產生時，進行割包且相對濕度 控制在90%以上’子實體成長至適當大小即可採收。 比較例1 請參照表一’為添加舖地黍代料取代部分木屑之培養基與 木屑培養基栽培秀珍菇之出菇產量（生物效率）之比較。下 述的配方卜3中’均分別包含35%重量比的乾料以及65% 重量比的水°其中，配方1之乾料為含有30%重量比之鋪 地黍代料、60%重量比之木屑、9%重量比之米糠及1%重 量比之破酸釣’混合後之乾料再加水調整至水含量為65% 之培養基；配方2為含有45%重量比之鋪地黍代料、45〇/〇 重量比之木屑、9%重量比之米糠及1%重量比之碳酸鈣， 混合後之乾料再加水調整至水含量為65%之培養基；配方 3為含有60%重量比之鋪地黍代料、3〇%重量比之木屑、 9%重量比之米糠及1%重量比之碳酸鈣，混合後之乾料再 加水調整至水含量為65%之培養基；對照組為含有9〇%重 量比之木屑、9%重量比之米糠及1%重量比之碳酸鈣混 合後之乾料再加水調整至水含量為65%之培養基。生物效 率的計算方式係利用：生物效率（％)=新鮮子實體重量/培養 基乾重X 表一、添加S —1 . 100。 •地黍 生物效率（％、 ^分木肩之培養基 第二潮菇 生物效率（％) 栽培秀珍菇之生马 第三潮菇 生物效率（％) 为效率比較 總生物效率 (%) 配方1 ^.31+1.18 17.54 ± 1.58 8.81 ± 0.05 43.66 ± 0 94~~ 配方2 23.78 ± 0.94 22.74 ± 1.97 10.64 ±0.39 57.16 i 1 1 〇 配方3 15.1〇±1.3〇 -----—_ 18.86 ±2.28 12.77 ± 1.54 46.73 8 1328424 _ 99年4月27日修正替換 對照組 12.13 ± 1.62 16.50 ±0.24 10.92 ±1.14 39.55 ± 1.001328424 Modified on April 27, 1999, IX. Description of the Invention: [Technical Field] The present invention relates to a culture medium for cultivating mushrooms, and in particular to a medium formulation for cultivating mushrooms . [Prior Art] The global mushroom industry has flourished in recent decades, and mushroom production has increased year by year. Since most of the mushrooms belong to woody fungi, the main medium is broadleaf trees. 'Requires considerable forest resources to satisfy mushrooms. The needs of the industry, so the development of the mushroom industry has also led to the problem of "bacterial forest contradiction" between the mushroom production and the forestry ecological balance. 'The mushroom industry is also bound to shrink with the shrinking forest area' and it faces the inability of this industry to continue to develop. Dilemma. The current improvement method is to replace the woody culture medium with the mushroom culture waste to cultivate other benefits, and at the same time develop the technology of replacing the wood genus with the genus. The so-called fungus is a herb that has been proved to be suitable for cultivation of food and medicinal mushrooms by scientific experiments. At present, it has formed another industry, the grass industry, and the development of the fungus industry has relatively eased the population, food, resources, Environmental, energy and other issues. Pleurotus genus (corpse spp.) is the third highest mushroom in the world. The hyphae of this genus grows fast, and it has strong ability to decompose cellulose and lignin. Xiu Jinzi (P/ and Jinding Pleurotus (Zero (9) (10) Curry Heart Plus Milk) is a mushroom of Pleurotus ostreatus. In Taiwan, Pleurotus ostreatus is the fourth largest strain, and Jinding Pleurotus is a new strain in recent years. Because it is easy to cultivate and delicious, it is very popular among growers and consumers. 5 1328424 April 27, 1999 Revision of the past related reports have pointed out that not only herbs, woody plants can be used as Pleurotus ostreatus Growth matrices, agricultural waste or other lignocellulosic components can also be used as growth matrices. Compared to common sawdust culture media, if the appropriate mushroom waste growth substrate or herbaceous plant parts are substituted for wood chips, it can be improved. [Effect of the Invention] Therefore, the present invention provides a medium formula for cultivating mushrooms in the field for the purpose of improving the yield of Pleurotus ostreatus. According to an embodiment of the present invention, a mushroom is proposed. The medium formulation comprises 35% by weight of the dry portion and 65% by weight of the water, wherein the dry portion comprises 30 to 60% by weight of the weeding straw, 3 to 60% by weight The wood chips '9% by weight of rice bran and 1% by weight of carbonic acid. The overall water content of the medium formulation is about 65%." The culture medium formula of the cultivated mushroom of the present invention is replaced by the wild herbaceous plant. The wood chips are used as the medium formula, and the mycelial growth rate and the biological efficiency are improved compared with the traditional formula using only wood chips as the medium, and the purpose of increasing the yield of the Pleurotus ostreatus can be effectively achieved. For example, the Pleurotus ostreatus was observed, and the mycelial growth rate, fruit body yield, and bio-efficiency of the substrate were replaced with wood chips as a medium formula. According to an embodiment of the present invention, the test strains (Pleurotus ostreatus and Pleurotus ostreatus) were tested. 6 1328424 On April 27, 1999, the revised replacement system was stored in 4 refrigerators. When the test was carried out, the mycelial pieces were inoculated into a potato glucosinolate culture medium (PDA) culture dish, activated in a 25 ° C incubator, and activated again. After the strain, the mycelial mass can be inoculated into the wheat grain medium. The preparation of the wheat grain medium is first washed with water and placed in water overnight, • filtered After the separation, add 1% rice bran and 0.5% calcium carbonate, mix well and then inject 500 ml of plastic bacteria bottle into high temperature and high pressure steam steel (12TC, 1.5 kg/cm2) for 80 minutes, then cool and use. The granule culture medium after the mycelium is cultured in a 25eC incubator, and shaken occasionally during the culture to avoid agglomeration of the granules. After 14 days, the mycelium is overgrown with the surface of the granules, which is the bacterium. The mantle substitutes pulverize the puddles collected in the field or in the field by a pulverizer, and sift through the straws with a length of 2 cm or less, and then bag them for drying. Mixing at least three months of broadleaf tree sawdust (Mountain jute, nanmu, acacia, phoenix wood) and paving sorghum substitutes to make several different blends of medium formula, the water content of the medium is Adjusted at around 65%. The well-mixed medium was placed in a space bag plastic bag every 1100 g, sterilized by 1.5 kg/cm2, 12PC for 80 minutes, and after cooling, the granules were inserted into different mediums. The culture medium after inoculation was placed in its culture chamber, and the mycelial culture was carried out without light, and the relative humidity was controlled at 7〇 75%. Among them, after the hyphae of the Pleurotus ostreatus was full of space bags, the space bag was moved to 18 ° C for 3 days after stimulation for 2 days, the temperature was adjusted back to 25t:, and the bag was cut, and the relative humidity was controlled at 9G% after the production of Zlei. Above, the sub-entities continue to grow 'up to the appropriate size to harvest. 7 1328424 ---- Correction and replacement on April 27, 1999 When the golden ear was cultured to the original substrate, the dicing was carried out and the relative humidity was controlled at 90% or more. Comparative Example 1 Referring to Table 1 'Comparison of the yield of the mushrooms (bioefficiency) of the cultivated oyster mushrooms in the medium in which the swarf was replaced by the addition of the slabs. In the following formula 3, 'each contains 35% by weight of dry material and 65% by weight of water respectively. Among them, the dry matter of Formula 1 is 30% by weight of paving substitute, 60% by weight. Wood chips, 9% by weight of rice bran and 1% by weight of acid-salted fish, 'mixed dry material is added with water to adjust the water content to 65% of the medium; Formula 2 is 45% by weight of the paving substitute 45 〇 / 〇 weight ratio of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, the mixed dry material is added with water to adjust the water content of 65% of the medium; Formulation 3 is 60% by weight The paving material, 3〇% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, and the mixed dry material is further adjusted to a medium with a water content of 65%; the control group is The dry matter mixture containing 9% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate was added to water to adjust the medium to a water content of 65%. Bioefficiency is calculated using: Bioefficiency (%) = fresh fruit body weight / culture basis dry weight X Table 1, adding S - 1. 100. • Mantle bio-efficiency (%, ^ divided wood shoulder medium second mushroom bio-efficiency (%) cultivated Pisces mushroom, third horse mushroom bio-efficiency (%) for efficiency comparison total bio-efficiency (%) Formula 1 ^ .31+1.18 17.54 ± 1.58 8.81 ± 0.05 43.66 ± 0 94~~ Recipe 2 23.78 ± 0.94 22.74 ± 1.97 10.64 ±0.39 57.16 i 1 1 〇Formulation 3 15.1〇±1.3〇------_ 18.86 ±2.28 12.77 ± 1.54 46.73 8 1328424 _ April 27, 1999 revised replacement control group 12.13 ± 1.62 16.50 ± 0.24 10.92 ± 1.14 39.55 ± 1.00
由表一可看出，在利用添加鋪地黍代料取代部分木屑 之培養基栽培秀珍菇的出菇生物效率方面，本試驗採收三 潮菇，總計對照組生物效率為39.55%，而以鋪地黍代料取 代木屑之三種不同比例的配方培養基的出菇生物效率均 較對照組高，其中，以配方2 (含有45%重量比之鋪地黍 代料及45%重量比之木屑）栽培的秀珍菇之生物效率較 高，為 57.16%。 比較例2 表二為添加鋪地黍代料取代部分木屑之培養基與木 屑培養基栽培秀珍菇之菌絲生長速率比較。下述的配方 1-3中，均分別包含35%重量比的乾料以及65%重量比的 水。其中，配方1之乾料為含有30%重量比之鋪地黍代料、 60%重量比之木屑、9%重量比之米糠及1 %重量比之碳酸 鈣，混合後之乾料再加水調整至水含量為65%之培養基； 配方2為含有45%重量比之鋪地黍代料、45%重量比之木 屑、9%重量比之米糠及1%重量比之碳酸鈣，混合後之乾 料再加水調整至水含量為65%之培養基；配方3為含有 60%重量比之鋪地黍代料、30%重量比之木屑、9%重量比 之米糠及1%重量比之碳酸鈣，混合後之乾料再加水調整 至水含量為65%之培養基；對照組為含有90%重量比之木 屑、9%重量比之米糠及1%重量比之碳酸鈣，混合後之乾 料再加水調整至水含量為65%之培養基。 9 1328424 _ 99年4月27日修正替換 表二'以鋪地黍代料栽培秀珍菇之菌絲生長速率比較 菌絲密度及外觀型態 菌絲生長速率(mm/day) 配方1 顏色雪白均一 7.4 ±0.8 配方2 顏色雪白均一 8.0 ±0.8 配方3 顏色雪白均一 5.5 ±0.9 對照組 顏色雪白均一 7.2 ±0.5It can be seen from Table 1 that in the experiment, the bio-efficiency of the mushroom cultivation of the cultivar was replaced by the addition of the paving sputum substitute medium, and the bio-efficiency of the control group was 39.55%. The biogas efficiency of the three different proportions of the formula medium replaced by the mantle substitute was higher than that of the control group, and the formula 2 (containing 45% by weight of the paving substitute and 45% by weight of the wood chips) was cultivated. The biological efficiency of Xiuzhen Mushroom is 57.16%. Comparative Example 2 Table 2 compares the mycelial growth rates of the medium in which the wood cuttings are replaced with the wood chips and the wood pulp culture medium. Each of the following Formulas 1-3 contained 35% by weight of dry matter and 65% by weight of water, respectively. Among them, the dry material of Formula 1 is 30% by weight of paving substitute, 60% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, and the mixed dry material is adjusted with water. To a medium with a water content of 65%; Formulation 2 is a 45% by weight of paving substitute, 45% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, mixed and dried The water is adjusted to a medium with a water content of 65%; Formulation 3 is a 60% by weight of the paving substitute, 30% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate. The mixed dry material was adjusted with water to adjust to a medium with a water content of 65%; the control group contained 90% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, and the mixed dry matter was further added with water. Adjust to a medium with a water content of 65%. 9 1328424 _ April 27, 1999 Amendment to Table 2 'The mycelial growth rate of cultivated Pleurotus ostreatus by paving sorghum substitutes. Comparison of hyphal density and appearance of hyphal growth rate (mm/day) Formula 1 Color snow white uniform 7.4 ±0.8 Formula 2 Color Snow White Uniform 8.0 ±0.8 Formula 3 Color Snow White Uniform 5.5 ± 0.9 Control Color Snow White Uniform 7.2 ±0.5
由表二可看出，在利用添加鋪地黍代料取代部分木屑 之培養基栽培秀珍菇的菌絲生長方面，不同培養基的菌絲 生長顏色均為雪白且均一，和以木屑為主的對照組相較並 無差異。而生長速率方面，以配方2(含有45%重量比之 鋪地黍代料及45%重量比之木屑）栽培的秀珍菇生長較快。 比較例3 表三為添加鋪地黍代料取代部分木屑之培養基與木 屑培養基栽培金頂側耳之出菇產量（生物效率）之比較。下 述的配方1-3中，均分別包含35%重量比的乾料以及65% 重量比的水。其中，配方1之乾料為含有30%重量比之鋪 地黍代料、60%重量比之木屑、9%重量比之米糠及1%重 量比之碳酸鈣，混合後之乾料再加水調整至水含量為65% 之培養基；配方2為含有45%重量比之鋪地黍代料、45% 重量比之木屑、9%重量比之米糠及1%重量比之碳酸鈣， 混合後之乾料再加水調整至水含量為65%之培養基；配方 3為含有60%重量比之鋪地黍代料、30%重量比之木屑、 9%重量比之米糠及1%重量比之碳酸鈣，混合後之乾料再 加水調整至水含量為65%之培養基；對照組為含有90%重 10 1328424 _ 99年4月27日修正替換 量比之木屑、9%重量比之米糠及1 %重量比之碳酸妈，混 合後之乾料再加水調整至水含量為65%之培養基。 表三、添加鋪地黍代料取代部分木屑之培養基栽培金頂側耳之生物效率比較 生物效率 配方1 配方2 配方3 對照組 第一潮兹 7.13% ±2.61% 8.42% ± 3.46% 11.21% ±2.84% 7.22% ± 3.01% 第二潮菇 10.15% ±3.84% 13.24% ±4.19% 11.53% ±3.27% 9.27% ± 2.61% 第三潮菇 12.05% ± 4.30% 11.73% ±4.25% 10.33% ± 1.76% 6.10% ±1.87% 第四潮菇 8.36% ± 1.65% 9.25% ±2.31% 7.62% ±2.31% 6.01% ± 1.23% 第五潮菇 7.80% ± 3.20% 7.85% ± 2.84% 9.79% ± 2.34% 6.46% ± 1.72% 第六潮菇 12.27% ±8.83% 14.35% ± 4.52% 13.39% ±8.12% 9.04% ± 6.02% 第六潮菇後 7.13% ±2.61% 8.42% ± 3.46% 11.21% ±2.84% 7.22% ±3.01% 總生物效率 57.75 % 64.84 % 63.88 % 44.09 %It can be seen from Table 2 that the hyphal growth of different culture media is white and uniform in the cultivation of mycelial growth of Pleurotus ostreatus by using the added paving substitute to replace part of the wood chips, and the control group based on sawdust. There is no difference. In terms of growth rate, the cultivated mushrooms cultivated in Formula 2 (containing 45% by weight of the paving substitute and 45% by weight of wood chips) grew faster. Comparative Example 3 Table 3 compares the yield of the mushrooms (the bioefficiency) of the cultured golden stalks of the scutellaria chinensis with the addition of the mulch substitute. In the following Formulations 1-3, each contained 35% by weight of dry matter and 65% by weight of water, respectively. The dry material of the formula 1 is 30% by weight of the paving substitute, 60% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, and the mixed dry material is adjusted with water. To a medium with a water content of 65%; Formulation 2 is a 45% by weight of paving substitute, 45% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate, mixed and dried The feed was adjusted to a medium with a water content of 65%; Formulation 3 was a 60% by weight of paving substitute, 30% by weight of wood chips, 9% by weight of rice bran and 1% by weight of calcium carbonate. The mixed dry material was adjusted with water to adjust to a medium with a water content of 65%; the control group was containing 90% by weight 10 1328424 _ April 27, 1999 corrected replacement ratio of wood chips, 9% by weight of rice bran and 1% by weight Compared with the carbonic acid mother, the mixed dry material was adjusted to a medium with a water content of 65%. Table 3: Bio-efficiency of the cultivation of Pleurotus ostreatus in the medium supplemented with some wood chips. Bio-Efficiency Formula 1 Formulation 2 Formulation 3 The first tide of the control group was 7.13% ± 2.61% 8.42% ± 3.46% 11.21% ± 2.84 % 7.22% ± 3.01% Second mushroom 13.15% ±3.84% 13.24% ±4.19% 11.53% ±3.27% 9.27% ± 2.61% Third mushroom 12.05% ± 4.30% 11.73% ±4.25% 10.33% ± 1.76% 6.10 % ±1.87% The fourth mushroom 8.36% ± 1.65% 9.25% ±2.31% 7.62% ±2.31% 6.01% ± 1.23% The fifth mushroom 7.80% ± 3.20% 7.85% ± 2.84% 9.79% ± 2.34% 6.46% ± 1.72% sixth mushroom 13.27% ±8.83% 14.35% ± 4.52% 13.39% ±8.12% 9.04% ± 6.02% 7.13% after the sixth mushroom 2.63% ±2.61% 8.42% ± 3.46% 11.21% ±2.84% 7.22% ±3.01 % Total bioefficiency 57.75 % 64.84 % 63.88 % 44.09 %
由表三可看出，在利用添加鋪地黍代料取代部分木屑 之培養基栽培金頂側耳的出菇生物效率方面，本試驗採收 六潮菇以上，總計對照組生物效率為44.09%，而以鋪地黍 代料取代木屑之三種不同比例的配方培養基的出菇生物 效率均較對照組高，其中，以配方2 (含有45%重量比之 鋪地黍代料及45%重量比之木屑）栽培的金頂側耳之生物 效率較高，為64.84%。 比較例4 表四為添加鋪地黍代料取代部分木屑之培養基與木 屑培養基栽培金頂側耳之菌絲生長速率比較。下述的配方 1-3中，均分別包含35%重量比的乾料以及65%重量比的 水。其中，配方1之乾料為含有30%重量比之鋪地黍代料、 60%重量比之木屑、9%重量比之米糠及1 %重量比之碳酸 11 1328424 _ 99年4月27日修正替換It can be seen from Table 3 that in the experiment, the bio-efficiency of the mushroom cultivation of Pleurotus ostreatus was replaced by the addition of the paving sputum substitute medium, and the bio-efficiency of the control group was 44.09%. The bioavailability of the three different proportions of the formulating medium in which the wood chips were replaced by the wood substitute was higher than that of the control group, and the formula 2 (containing 45% by weight of the paving substitute and 45% by weight of the wood chips) The bio-efficiency of the cultivated Pleurotus ostreatus was 64.84%. Comparative Example 4 Table 4 compares the mycelial growth rates of the culture medium with the addition of the paving substitute to replace the portion of the wood chips with the wood chip culture medium. Each of the following Formulas 1-3 contained 35% by weight of dry matter and 65% by weight of water, respectively. Among them, the dry material of Formula 1 is 30% by weight of paving substitute, 60% by weight of wood chips, 9% by weight of rice bran and 1% by weight of carbonic acid 11 1328424 _ April 27, 1999 amendment replace
鈣，混合後之乾料再加水調整至水含量為65%之培養基； 配方2為含有45%重量比之鋪地黍代料、45%重量比之木 屑、9%重量比之米糠及1%重量比之碳酸鈣，混合後之乾 料再加水調整至水含量為65%之培養基；配方3為含有 60%重量比之鋪地黍代料、30%重量比之木屑、9%重量比 之米糠及1%重量比之碳酸鈣，混合後之乾料再加水調整 至水含量為65%之培養基；對照組為含有90%重量比之木 屑、9%重量比之米糠及1%重量比之碳酸鈣，混合後之乾 料再加水調整至水含量為65%之培養基。 表四、以鋪地黍代料栽培金頂側耳之菌絲生長速率比較 菌絲密度及外觀型態 菌絲生長速率(mm/day) 配方1 顏色雪白均一 7.1 ±0.4 配方2 顏色雪白均一 7.3 ± 1.0 配方3 顏色雪白均一 6.5 ± 0.9 對照組 顏色雪白均一 7.0 ± 0.6 由表四可看出，在利用添加铺地黍代料取代部分木屑 之培養基栽培金頂側耳的菌絲生長方面，不同培養基的菌 絲生長顏色均為雪白且均一，和以木屑為主的對照組相較 並無差異。而生長速率方面，以配方2 (含有45 %重量比 之鋪地黍代料及45%重量比之木屑）栽培的金頂側耳生長 較快。 根據上述比較例，以30〜60%重量比之鋪地黍作為代 料取代部分木屑所製成之培養基可適用於栽培菇類，特別 是以45%重量比與45%重量比之木屑昆合的培養基，應用 於側耳屬菇類時，更能有效提高菌絲生長速度和出菇效 率。因此，可依實際需求，利用本發明實施例之培養基， 12 1328424 _ • 99年4月27日修正替換 _____________ 配合調整適當的鋪地黍代料比例來增進菌絲生長速度、出 I去效率’進而達到增加菇類產量的目的。 雖然本發明已以數個實施例揭露如上，然其並非用以 限定本發明，任何熟習此技藝者，在不脫離本發明之精神 和範圍内，當可作各種之更動與潤飾，因此本發明之保護 範圍當視後附之申請專利範圍所界定者為準。 【圖式簡單說明】 響 無 【主要元件符號說明】 鉦 *、、、Calcium, the mixed dry material is adjusted to a medium with a water content of 65%; Formulation 2 is a 45% by weight of paving substitute, 45% by weight of wood chips, 9% by weight of rice bran and 1% The weight ratio of calcium carbonate, the mixed dry material is added with water to adjust the medium to a water content of 65%; the formula 3 is 60% by weight of the paving substitute, 30% by weight of wood chips, 9% by weight Rice bran and 1% by weight of calcium carbonate, the mixed dry material was adjusted with water to a medium with a water content of 65%; the control group contained 90% by weight of wood chips, 9% by weight of rice bran and 1% by weight. Calcium carbonate, the mixed dry material was adjusted with water to a medium having a water content of 65%. Table 4: Mycelial growth rate of Pleurotus ostreatus cultivated by paving sorghum substitutes. Comparison of hyphal density and appearance hyphal growth rate (mm/day) Formula 1 Color snow white uniform 7.1 ± 0.4 Formula 2 Color snow white uniform 7.3 ± 1.0 Formula 3 Color Snow White Uniform 6.5 ± 0.9 Control Color Snow White Uniform 7.0 ± 0.6 As can be seen from Table 4, different mediums were used for the cultivation of mycelial growth of Pleurotus ostreatus in a medium that replaced some wood chips with added paving substitutes. The mycelial growth color was white and uniform, and there was no difference between the control group with wood chips. In terms of growth rate, the Pleurotus ostreatus cultivated in Formula 2 (containing 45% by weight of the buckwheat substitute and 45% by weight of the wood chips) grew faster. According to the above comparative example, the medium prepared by substituting a part of the wood chips with a weight ratio of 30 to 60% by weight as a substitute can be applied to the cultivation of the mushroom, especially in the case of 45% by weight and 45% by weight of the wood chips. The medium, when applied to Pleurotus ostreatus, is more effective in increasing mycelial growth rate and mushrooming efficiency. Therefore, according to actual needs, the medium of the embodiment of the present invention can be used, 12 1328424 _ • April 27, 1999, correction and replacement _____________, and adjusting the proportion of appropriate paving materials to improve the mycelial growth rate and the efficiency of the I go. 'To achieve the purpose of increasing the production of mushrooms. While the present invention has been described above in terms of several embodiments, it is not intended to limit the invention, and the invention may be modified and modified without departing from the spirit and scope of the invention. The scope of protection is subject to the definition of the scope of the patent application. [Simple description of the diagram] No No [Description of main component symbols] 钲 *, ,,
1313